CN106698646A - Comprehensive polluted surface water body remediation device and method based on immobilized cell technology - Google Patents

Abstract

The invention provides a comprehensive polluted surface water body remediation device and method based on an immobilized cell technology. A control box is installed in the middle of a fixing frame, float groups are symmetrically installed in the portions, at the periphery of the control box, the portion, on the top of each float group, of the fixing frame is provided with a solar cell panel, the portion, at the bottom of each float group, of the fixing frame is provided with a biological box, and each biological box is uniformly provided with multiple small side wall holes; a photovoltaic controller is installed in the control box and connects the solar cell panels with a storage battery, the storage battery supplies power to an aeration mechanism, and the aeration mechanism achieves an oxygen supply function for microorganisms. According to the device, installation and operation are easy, in-situ bioremediation can be conducted on a water body, the immobilized cell technology can be assisted, therefore, the microorganisms cannot be free to be dispersed, and the effects of a dominant bacterial community are embodied and exerted. More microorganisms can be enriched by an artificial aquatic mat through the large specific surface area advantage of the artificial aquatic mat, and therefore the remediation effect of the device on the polluted water body is enhanced.

Description

Device and method for comprehensive restoration of polluted surface water based on immobilized cell technology

technical field

The invention belongs to the field of water quality purification, and relates to equipment and methods for repairing and purifying polluted surface water bodies, in particular to a comprehensive repairing device and method for polluted surface water bodies based on immobilized cell technology.

Background technique

Due to the rapid development of industry and agriculture, limited surface water resources are subject to varying degrees of pollution, the ecological environment of water bodies is deteriorating day by day, and biodiversity is severely damaged, which affects human life and restricts economic development. Therefore, the remediation of polluted surface water has become a realistic and urgent environmental protection problem. Polluted surface water has the characteristics of wide pollution channels, high fluidity, strong seasonality, and low pollution concentration, and bioremediation is commonly used. The traditional bioremediation method uses microorganisms to degrade pollutants in water to purify water bodies, but the lack of carriers for microorganisms leads to their free dispersion, making it difficult to form dominant bacterial groups, unable to efficiently treat water bodies, and there is also the problem of insufficient dissolved oxygen. At present, artificial wetlands and ecological floating island methods are widely used in China, but such technologies are greatly affected by plants and climate. Aeration and oxygenation are commonly used in the treatment of polluted surface water. Aeration is used to increase dissolved oxygen in water to improve water quality, but aeration equipment consumes a lot of energy and investment costs are high.

The problem of surface water pollution is the product of the rapid development of modern industry and agriculture, especially the widespread use of chemical fertilizers has led to the accumulation of a large number of nitrogen-containing compounds in the natural environment, resulting in serious eutrophication of surface water bodies. The massive reproduction of algae and other plankton leads to a rapid decline in dissolved oxygen, causing other aquatic organisms to die due to hypoxia and suffocation, directly destroying the aquatic ecosystem and affecting economic benefits, and the phenomenon of water quality-type water shortage due to pollution is gradually intensified. Therefore, the treatment and restoration of polluted surface water has become a realistic and urgent environmental protection issue.

In most eutrophic water bodies, due to the low carbon/nitrogen ratio, it is difficult to effectively remove nitrogen and phosphorus in the water. At present, many countries are widely using biological treatment measures to treat sewage, and polluted surface water has the characteristics of wide pollution channels, high fluidity, strong seasonality, and low pollution concentration, and its components are becoming more complex. Traditional bioremediation technology shows many Defects, such as insufficient microbial biomass and insufficient oxygenation.

Contents of the invention

In view of the deficiencies in the prior art, the object of the present invention is to provide a comprehensive restoration device for polluted surface water, which solves the deficiencies of the traditional bioremediation method and the defects of a single technology.

Another object of the present invention is to provide a method for comprehensive restoration of polluted surface water based on immobilized cell technology, and cooperate with the above-mentioned comprehensive restoration device for polluted surface water to better solve the problem of bioremediation of polluted water.

In order to solve the above technical problems, the present invention adopts the following technical solutions to achieve:

A comprehensive restoration device for polluted surface water, comprising a fixed frame, a control box is installed in the middle of the fixed frame, buoy groups are symmetrically installed in the fixed frame around the control box, and solar cells are installed on the fixed frame at the top of each buoy group A biological box is installed on the fixed frame at the bottom of each buoy group, and a plurality of side wall small holes are evenly processed on the biological box;

A photovoltaic controller is installed in the control box, and the photovoltaic controller connects the solar panel and the storage battery, and the storage battery supplies power to the aeration mechanism.

The present invention also has the following distinguishing technical features:

Further, the bottom of the biological box is provided with a suspension frame, and artificial aquatic plants are suspended on the suspension frame.

Specifically, the aeration mechanism includes an air inlet processed on the side wall of the control box, and also includes a motor connected to the battery installed in the control box. The motor is connected with one end of the hollow stirring shaft to drive the hollow stirring shaft to rotate, and the control box The hollow stirring shaft inside is processed with a vent hole, which communicates with the hollow cavity of the hollow stirring shaft. The other end of the hollow stirring shaft protrudes from the bottom of the control box and is connected with a negative pressure chamber at the end. A stirring paddle capable of generating negative pressure in the negative pressure chamber.

Preferably, the hollow stirring shaft has a segmented structure, and each segment is connected by a coupling.

Preferably, the negative pressure chamber is trumpet-shaped, and the side wall of the negative pressure chamber is provided with small holes.

Preferably, the buoy groups are four groups symmetrically arranged in a matrix.

The present invention also provides a method for comprehensive restoration of polluted surface water based on immobilized cell technology. The method adopts the above-mentioned comprehensive restoration device for polluted surface water to restore polluted surface water.

Specifically, the method specifically includes the following steps:

Step 1, preparation of matrix solution:

Configure a matrix solution, the COD in the matrix solution is 500mg/L, the ammonia nitrogen concentration is 20mg/L, and the phosphate concentration is 10mg/L;

Step 2, preparation of bacterial suspension:

Take the sludge mixture from the local sewage plant to treat the polluted surface water. In this example, the suspended solids concentration of the mixture is 3000 mg/L, and the sludge mixture is inoculated on the matrix prepared in step 1 at a volume ratio of 30%. In the fermentation tank of the solution, add nutrients so that the concentration of the yeast extract in the fermentation tank is 50mg/L, and the trace inorganic salts in the fermentation tank are (NH ₄ ) ₂ SO ₄ , KH ₂ PO ₄ , MgSO ₄ , FeSO ₄ and _CaCl2 ;

Aeration and acclimatization at a constant temperature at 30°C, changing the water every 2 to 3 days, and detecting the contents of COD, ammonia nitrogen and total phosphorus, when the degradability of the domesticated strains to COD, ammonia nitrogen and total phosphorus reaches more than 90%, Carry out expanded culture, insert the strain into LB medium (yeast extract 5g/L, NaCl 10g/L, peptone 10g/L), culture in a fermenter with constant temperature and aeration at 30°C for 2 days, and let the cultured bacterial liquid stand Precipitate for 24 hours, discard the supernatant, wash with phosphate buffer, discard the supernatant after the same static precipitation, and suspend the bacteria in the phosphate buffer to form a bacterial suspension. The microbial content in the bacterial suspension is 20×10 ¹¹ to 30×10 ¹¹ pieces.mL ^-1 ;

Step 3, embedding and immobilization of microorganisms:

A sodium alginate solution with a concentration of 4% was prepared as an embedding material, and after it was completely dissolved, it was uniformly mixed with the bacterial suspension prepared in step 2 in equal volume. The mixed solution was poured into the loofah with a length of about 30 cm, and then put into a 4% CaCl ₂ solution for cross-linking for 18 hours. After the cross-linking is completed, the loofah pulp is washed with sterile water to obtain the loofah pulp adsorbed with immobilized microorganisms;

Step four, fix:

Fill the biological box with the loofah pulp with immobilized microorganisms adsorbed therein obtained in step 3, then put the comprehensive restoration device for polluted surface water into the polluted surface water for suspension, and turn on the aeration mechanism for restoration.

Preferably, 4 artificial aquatic plants are hung below each biological box, and the diameter of the artificial aquatic plants is 5 cm and the length is 1.5 m.

Preferably, the aeration mechanism keeps the dissolved oxygen content in the water around the device above 4mg/L.

Compared with the prior art, the present invention has the following technical effects:

(I) The device of the present invention is easy to install and operate, and can perform in-situ bioremediation of water bodies. The device can assist immobilized cell technology, so that microorganisms will not be free and dispersed, and the role of dominant flora can be reflected and played. In addition, the use of aeration equipment to provide sufficient oxygen optimizes the underwater treatment environment. The application of solar panels highlights the characteristics of zero energy consumption and green environmental protection of the device. For the surface water body with low carbon/nitrogen ratio and serious eutrophication, the device is highly comprehensive and has a remarkable restoration effect.

(II) Artificial aquatic plants are a kind of biofilm carrier technology, which is rising day by day and has a good development prospect. In this device, it is fixed underwater and works together with immobilized microorganisms at different depths underwater. While improving the ecological environment of the water body, it can also increase the activity of microorganisms and enrich the growth of dominant bacteria. The dominant flora flowing out from the small holes on the side wall of the biological box can be enriched on the artificial aquatic plants, which can further strengthen the restoration effect on the water body. Artificial aquatic plants have a wide range of applications, good purification effects, low investment, simple operation and management, and quick results. Moreover, this technology is not affected by the growth time and climate, does not require special care, and is easy to operate and manage.

(Ⅲ) The microorganisms are immobilized by using a gel-embedded immobilization method. The method is simple to operate, has little influence on cell activity, and the immobilized cell spheres produced have high strength. Utilizing the limitation of oxygen diffusion, there are aerobic regions and anoxic regions in the immobilized cell particles, which meet the different requirements of nitrification and denitrification reactions on environmental conditions. Pollutants are degraded and eliminated in situ without secondary pollution or transfer of pollutants. In addition, the repair cost is less, only 30% to 50% of chemical repair and physical repair.

(Ⅳ) Choose loofah pulp as the adsorption carrier, and fix the cultured microorganisms in the loofah pulp after being embedded in the embedding agent. The small adsorption force leads to a large loss of immobilized bacteria. In addition, this microcapsule structure makes the carrier itself have a larger specific surface area, which further enhances the ability of bacteria to adsorb pollutants. Loofah seeds are economical, environmentally friendly and easy to obtain, and are excellent adsorption carriers.

(Ⅴ) The present invention can make a large amount of microorganisms attach and grow on the loofah carrier, and easily form a dominant flora; artificial aquatic plants use their own large specific surface area as a biofilm carrier; underwater aeration increases the oxygen content in water and promotes the growth of aerobic microorganisms Reproduction, enhance the effect of artificial aquatic plants hanging film. The combination of the three can effectively remove organic matter, nitrogen, phosphorus and other elements in water at different depths underwater, and the treatment effect is better and faster.

(Ⅵ) A single sewage treatment technology can no longer meet people's requirements for water quality. In order to overcome the shortcomings of a single technology, the present invention combines the immobilized cell technology, artificial aquatic plants and aeration and oxygenation, and fully utilizes each technology. Features, while achieving a good treatment effect, but also reduce investment.

Description of drawings

Fig. 1 is a schematic diagram of the overall structure of the present invention.

Fig. 2 is a schematic diagram of the cross-sectional structure along line A-A of Fig. 1 .

Fig. 3 is a schematic diagram of the B-B sectional structure of Fig. 1 .

Fig. 4 is a left view structural diagram of Fig. 1 .

The meanings of each label in the figure are: 1-fixed frame, 2-control box, 3-pontoon group, 4-solar panel, 5-biological box, 6-small hole on the side wall, 7-photovoltaic controller, 8-battery , 9-aeration mechanism, 10-hanging frame, 11-artificial aquatic plants, 12-immobilized microorganisms, 13-gas;

(9-1)-air inlet, (9-2)-motor, (9-3)-hollow stirring shaft, (9-4)-air vent, (9-5)-negative pressure chamber, (9-6 )-stirring paddle, (9-7)-coupling.

The specific content of the present invention will be further explained in detail below in conjunction with the examples.

detailed description

Immobilized cell technology refers to a basic technology that uses physical or chemical means to locate intact cells in a limited space area and keep them active and reusable. The immobilized environment created by immobilized cell technology protects microbial cells, avoids the damage of other bacteria and toxic substances to cells, and artificially destroys the activity of biological enzymes, strengthens the stability of biochemical reactions, and the repair effect is obviously better than free bacteria. The key is to screen suitable carrier materials and determine optimized immobilization process conditions.

Artificial water grass is a new type of artificial synthetic material contact oxidation technology. It is a bionic water grass material designed by imitating natural aquatic plants with materials that are highly resistant to pollution, corrosion resistance, elasticity, toughness and flexibility. It is a carrier with a large specific surface area. . Through the succession of microorganisms themselves, a three-dimensional microbial ecosystem is formed on the surface of the carrier. A large number of microbial species and a complete food web on the surface make the metabolic efficiency of the microbial community for organic pollutants higher, and the effect of nitrogen and phosphorus removal is obvious.

Aeration Oxygenation Oxygenates the water body through aeration equipment to accelerate the reproduction of indigenous aerobic microorganisms and enhance the self-purification ability of the water body. However, microorganisms lack carriers in flowing water, and the loss is serious, which makes the repair effect of aeration and oxygenation poor.

The present invention combines the three technologies by designing a comprehensive device, and while overcoming the defects of the traditional repair method, fully exerts the performance of each process, highlights and optimizes the effect of the combination, and the processing method is more comprehensive.

Specific embodiments of the present invention are provided below, and it should be noted that the present invention is not limited to the following specific embodiments, and all equivalent transformations done on the basis of the technical solutions of the present application all fall within the scope of protection of the present invention.

Example 1:

According to the above technical scheme, as shown in Figures 1 to 4, this embodiment provides a comprehensive restoration device for polluted surface water, including a fixed frame 1, a control box 2 is installed in the middle of the fixed frame 1, and the surrounding area of the control box 2 is The buoy group 3 is symmetrically installed in the fixed frame 1 of each buoy group 3, and the solar cell panel 4 is installed on the fixed frame 1 at the top of each buoy group 3. A plurality of side wall small holes 6 are uniformly processed on the top;

A photovoltaic controller 7 is installed in the control box 2, and the photovoltaic controller 7 connects the solar panel 4 and the storage battery 8, and the storage battery 8 supplies power for the aeration mechanism 9.

The size of the solar panel 4 is 1200×1000×50 mm, and the installation angle is 34.30 degrees.

The photovoltaic controller 7 is an automatic control device used in a solar power generation system to control the multi-channel solar battery array to charge the storage battery and the storage battery to supply power to the solar inverter load. The photovoltaic controller 7 may be a photovoltaic controller commonly used in the field.

The buoy group 3 adopts buoys to form, and the buoys are high-density solid core cylindrical foam buoys, and the buoyancy of each buoy can reach 120kg, and three buoys are installed below each solar panel 4 . The weight of the whole device is about 240kg, and the total weight is much smaller than the buoyancy of the buoy.

In this embodiment, as a preferred situation, the buoy groups 3 are four groups arranged symmetrically in a matrix, and four corresponding solar panels 4 are installed in total. The solar panel 4 is on the top of the entire device, and by absorbing sunlight, The solar radiant energy is converted into electric energy, thereby driving the aeration mechanism 9 to operate, so as to increase the dissolved oxygen in the water.

The immobilized microorganism 12 is placed in the biological box 5, and the casing of the biological box 5 is made of light material, and the side cover of the biological box 5 can be opened, which is convenient for placing the immobilized microorganism 12. Through the small hole 6 on the side wall, the water body can freely enter and exit the biological tank 5, and the carrier of the immobilized microorganisms 12 cannot pass through, so that the microorganisms can effectively degrade pollutants and restore the water body.

The size of the biological box 5 is 600×600×300mm. As a preference, the number of small holes 6 on the side walls of each biological box 5 is 36.

The immobilized microorganisms 12 are fixed by cell embedding and allowed to survive on specific biological carriers.

The bottom of the biological box 5 is provided with a suspension frame 10 on which artificial aquatic plants 11 are suspended. The artificial aquatic plants 11 are braided artificial aquatic plants, and the tubular diameter is variable during oxygenation without blockage. After being tied, it can be used for more than three years without special care. The number and length of the artificial aquatic plants can be selected according to the specific situation. The microorganisms escaping from the biological box 5 can be enriched by the artificial aquatic plants 11, thereby strengthening the effect of the dominant flora.

Due to the effect of aeration, the artificial aquatic plants 11 can better form a film in an oxygenated environment, forming a bionic system to purify the water body. As a preference of this embodiment, the specific surface area of the artificial aquatic plants 11 is above 2000m ² /m ³ , and four artificial aquatic plants 11 are hung below each biological box.

The aeration mechanism 9 includes an air inlet 9-1 processed on the side wall of the control box 2, and also includes a motor 9-2 installed in the control box 2 and connected to the storage battery 8, and the connection between the motor 9-2 and the hollow stirring shaft 9-3 One end is connected to drive the hollow stirring shaft 9-3 to rotate, and the hollow stirring shaft 9-3 in the control box 2 is processed with a vent hole 9-4, and the vent hole 9-4 communicates with the hollow cavity of the hollow stirring shaft 9-3, and the hollow stirring shaft 9-3 The other end of the shaft 9-3 stretches out the bottom of the control box 2 and communicates with the negative pressure chamber 9-5 with the bottom end open at the end, and the hollow stirring shaft 9-3 is also equipped with a device that can make the negative pressure chamber 9-5 generate negative pressure. Pressed stirring paddle 9-6.

The hollow stirring shaft 9-3 is a segmented structure, and each segment is connected by a shaft coupling 9-7.

The negative pressure chamber 9-5 is trumpet-shaped, which facilitates the formation of a negative pressure environment and the discharge of air.

When the device of the present invention is in use, the immobilized microorganisms 12 are packed into the biological box 5, and the whole device is floated in the water. The solar panel 4 converts solar energy into electric energy and stores it in the storage battery 8, and the storage battery 8 drives the hollow stirring shaft 9-3. Rotation, the subsequent rotation of the stirring paddle 9-6 makes the water body below the stirring paddle 9-6 form a negative pressure, and then forms a negative pressure in the negative pressure chamber 9-5, and the air flows from the air inlet 9-1 under the action of the negative pressure. Inhale in the hollow chamber of hollow stirring shaft 9-3, and overflow from negative pressure chamber 9-5, to increase the oxygen content in water. The immobilized microorganisms 12 grow and reproduce in the biological box 5. As the amount of reproduction increases, some microorganisms will escape from the small holes 6 on the side wall and accumulate on the artificial aquatic plants 11, thereby strengthening the effect of the dominant flora and improving The remediation effect of microorganisms on polluted water bodies.

Aiming at the characteristics of polluted surface water and the deficiencies of traditional bioremediation methods, the present invention combines aeration technology, immobilized cell technology and artificial aquatic plant technology, uses solar panels to supply power for aeration, and designs a comprehensive repair device for polluted surface water , effectively solve the problems of insufficient microbial biomass, difficult survival and difficult management of aquatic plants, overcome the defects of single bioremediation, strong adaptability, environmental protection and no energy consumption, and the repair effect is remarkable.

The tests in the following examples and comparative examples are all carried out simultaneously in a certain polluted lake water in Xi'an. The lake water pH=7.4, the initial dissolved oxygen concentration is 0.7mg/L, and the total nitrogen concentration is 2.4mg/L. Class V level, COD is 35mg/L, and total phosphorus concentration is 0.13mg/L, which is at the surface class V level.

Example 2:

This embodiment provides a method for comprehensive restoration of polluted surface water based on immobilized cell technology. The method uses the comprehensive restoration device for polluted surface water as claimed in claim 1 to repair polluted surface water. The method specifically includes the following steps:

Step 1, preparation of substrate solution:

Configuration substrate solution, COD (chemical oxygen demand) in the substrate solution is 500mg/L, ammonia nitrogen concentration is 20mg/L, and phosphate concentration is 10mg/L; The deployment of COD in the substrate solution is to deploy by conventional organic matter Yes, as long as the COD in the matrix solution meets the required concentration.

Step 2, preparation of bacterial suspension:

Take the sludge mixture from the local sewage plant to treat the polluted surface water. In this example, the suspended solids concentration of the mixture is 3000 mg/L, and the sludge mixture is inoculated on the matrix prepared in step 1 at a volume ratio of 30%. In the fermenter of the solution, add nutrients so that the concentration of the yeast extract in the fermenter is 50mg/L, and the trace inorganic salts in the fermenter are (NH ₄ ) ₂ SO4, KH ₂ PO4, MgSO4, FeSO ₄ and CaCl ₂ ;

Aeration and acclimatization at a constant temperature at 30°C, changing the water every 2 to 3 days, and detecting the contents of COD, ammonia nitrogen and total phosphorus, when the degradability of the domesticated strains to COD, ammonia nitrogen and total phosphorus reaches more than 90%, Carry out expanded culture, insert the strain into LB medium (yeast extract 5g/L, NaCl 10g/L, peptone 10g/L), culture in a fermenter with constant temperature and aeration at 30°C for 2 days, and let the cultured bacterial liquid stand Precipitate for 24 hours, discard the supernatant, wash with 0.1mol/L phosphate buffer, discard the supernatant after the same static precipitation, and suspend the bacteria in the phosphate buffer to form a bacterial suspension. The microbial content in the medium is 20×10 ¹¹ to 30× ¹⁰ 11.mL ^-1 ;

Step 3, embedding and immobilization of microorganisms:

A sodium alginate solution with a concentration of 4% was prepared as an embedding material, and after it was completely dissolved, it was uniformly mixed with the bacterial suspension prepared in step 2 in equal volume. The mixed solution was poured into the loofah with a length of about 30 cm, and then put into a 4% CaCl ₂ solution for cross-linking for 18 hours. After the cross-linking is completed, the loofah pulp is washed with sterile water to obtain the loofah pulp adsorbed with immobilized microorganisms;

Step four, fix:

Fill the biological box with the sponge gourd pulp obtained in step 3 with immobilized microorganisms adsorbed, and hang 4 artificial aquatic plants below each biological box. The comprehensive restoration device is suspended in the polluted surface water, and the aeration mechanism is turned on. The aeration mechanism keeps the dissolved oxygen content in the water around the device above 4mg/L, and the speed is generally controlled at 1500rpm for restoration.

After 1 month of restoration, the water quality at 6m below the bio-box was tested. The concentration of dissolved oxygen rose to 4mg/L, the concentration of total nitrogen was 1.5mg/L, reaching the standard of Class IV on the surface, the COD was 20mg/L, and the concentration of total phosphorus It is 0.05mg/L, reaching the standard of Class III on the surface.

Example 3:

This example provides a method for comprehensive restoration of polluted surface water based on immobilized cell technology. The process steps of this method are the same as those in Example 1, the only difference being that artificial aquatic plants are not suspended at the bottom of the bio-box.

After 1 month of restoration, the water quality at 6m below the bio-box was tested, and the dissolved oxygen concentration was 4.1mg/L, the total nitrogen concentration was 1.3mg/L, the COD was 28mg/L, and the total phosphorus concentration was 0.07mg/L. Compared with the original water quality, it has been greatly improved, but the improvement effect is not as good as that of Example 2, indicating that the artificial aquatic plants have also promoted the water quality restoration effect.

Comparative example 1:

This comparative example provides a kind of remediation method of polluted surface water body, and other steps of this method are identical with embodiment 2, and difference is, there is not the content of step 1 to step 3 in embodiment 2 in this comparative example, only the same amount of loofah pulp Put it into the biological box, hang 4 artificial aquatic plants under each biological box, the diameter of the artificial aquatic plants is 5cm, and the length is 1.5m, then put the comprehensive restoration device of the polluted surface water body into the polluted surface water body and suspend it. repair.

After 1 month of restoration, the water quality at 6m below the bio-box was tested, and the dissolved oxygen concentration rose to 3.7mg/L, the total nitrogen concentration was 1.8mg/L, the COD was 25mg/L, and the total phosphorus concentration was 0.08mg/L , Compared with the original water quality, it has been greatly improved, but the improvement effect is not as good as the examples, indicating that the immobilized microorganisms have a strong promotion effect on water quality restoration.

Comparative example 2:

This comparative example provides a method for remediating polluted surface water bodies. The other steps of this method are the same as in Example 2, the difference is that there is no content from step 1 to step 3 in Example 2 in this comparative example. Put the loofah pulp, and do not hang artificial aquatic plants under the biological box, and then put the comprehensive restoration device for polluted surface water into the polluted surface water for suspension and repair.

After 1 month of restoration, the water quality at 6m below the bio-box was tested, and the dissolved oxygen concentration was 3.8mg/L, the total nitrogen concentration was 1.3mg/L, the COD was 30mg/L, and the total phosphorus concentration was 0.11mg/L. Compared with the original water quality, the water quality has been improved, but the improvement is not as good as in Examples 2 and 3, indicating that aeration can restore water quality to a certain extent, and the combination of immobilized microorganisms and artificial aquatic plants has a significant effect on improving water quality.

Comparative example 3:

This comparative example provides a kind of remediation method of polluted surface water body, and other steps of this method are identical with embodiment 2, and difference is, there is not the content of step 1 to step 3 in embodiment 2 in this comparative example, only the same amount of loofah pulp Put it into the biological box, and do not hang artificial aquatic plants under the biological box, and then put the comprehensive restoration device for the polluted surface water body into the polluted surface water body and suspend it for restoration.

After 1 month of restoration, the water quality at 6m below the bio-box was tested, and the dissolved oxygen concentration rose to 4mg/L, the total nitrogen concentration was 1.2mg/L, the COD was 28mg/L, and the total phosphorus concentration was 0.09mg/L. Compared with Comparative Example 2, the water quality has improved, indicating that the adsorption properties of loofah pulp can improve water quality, but the improvement is small.

Comparative example 4:

This comparative example provides a method for remediating polluted surface water bodies. The other steps of this method are the same as in Example 2, the difference is that there is no content from step 1 to step 3 in Example 2 in this comparative example. Put the loofah pulp, and only hang the artificial aquatic plants below the biological box. The artificial aquatic plants have a diameter of 5cm and a length of 1.5m.

After a month of restoration, the water quality at 6m below the bio-box was tested, and the concentration of dissolved oxygen rose to 4.1mg/L, the concentration of total nitrogen was 1.1mg/L, the concentration of COD was 24mg/L, and the concentration of total phosphorus was 0.08mg/L . Compared with Comparative Example 2, the water quality has been improved, but not as good as that of Example 2, which shows that the artificial aquatic plants can promote the restoration of water quality, and also shows that the immobilized microorganisms have an obvious effect on water purification.

Comparative example 5:

This comparative example provides a kind of restoration method of polluted surface water body, and other steps of this method are the same as embodiment 2, and difference is that in this comparative example, the stirring paddle is turned off, aeration is not carried out in the water body, and the oxygen in the water is not additionally increased. content.

After 1 month of restoration, it was found that the carrier in the biological box had been completely disintegrated. The water quality at 6m from the device was tested, and the dissolved oxygen concentration was 1.3mg/L, the total nitrogen concentration was 1.2mg/L, and the COD was 31mg/L. Phosphorus concentration is 0.08mg/L, which is improved compared with the original water quality, indicating that immobilized microorganisms and artificial aquatic plants can improve water quality, but aeration plays a strong role in promoting it.

Claims (10)

1. a kind of polluted surface water body comprehensive repair device, including fixed mount (1), it is characterised in that in described fixed mount (1) Portion is provided with control cabinet (2), is symmetrically installed with floating drum group (3) in the fixed mount (1) of control cabinet (2) surrounding, each floating drum group (3) Solar panel (4) is installed on the fixed mount (1) at top, life is installed on the fixed mount (1) of each floating drum group (3) bottom Thing case (5), is uniformly machined with multiple side wall apertures (6) on biological case (5)；

Photovoltaic controller (7) is installed, photovoltaic controller (7) is by solar panel (4) and electric power storage in described control cabinet (2) Pond (8) links together, and battery (8) is aeration mechanism (9) power supply.

2. polluted surface water body comprehensive repair device as claimed in claim 1, it is characterised in that described biological case (5) bottom Portion is provided with hanger bracket (10), and artificial aquatic weed (11) is hung with hanger bracket (10).

3. polluted surface water body comprehensive repair device as claimed in claim 1, it is characterised in that described aeration mechanism (9) Including processing the air intake (9-1) on the wall of control cabinet (2) side, also including in the control cabinet (2) with battery (8) phase Motor (9-2) even, motor (9-2) is connected with one end of hollow stirrer axle (9-3) and drives hollow stirrer axle (9-3) to rotate, control Passage (9-4), passage (9-4) and hollow stirrer axle (9-3) are machined with hollow stirrer axle (9-3) in case (2) processed Middle cavity connection, the other end of hollow stirrer axle (9-3) stretches out control cabinet (2) bottom and is communicated with bottom opening in end Negative pressure cavity (9-5), is also equipped with the agitating paddle (9- for enabling to negative pressure cavity (9-5) to produce negative pressure on hollow stirrer axle (9-3) 6)。

4. polluted surface water body comprehensive repair device as claimed in claim 3, it is characterised in that described hollow stirrer axle (9-3) is segmentation structure, is connected by shaft coupling (9-7) between each section.

5. polluted surface water body comprehensive repair device as claimed in claim 3, it is characterised in that described negative pressure cavity (9-5) For horn-like, negative pressure cavity (9-5) side wall is provided with aperture.

6. polluted surface water body comprehensive repair device as claimed in claim 1, it is characterised in that described floating drum group (3) is Four groups of matrix form symmetric arrays.

7. a kind of polluted surface water body method for comprehensive repairing based on immobilized cell technology, it is characterised in that the method is used Polluted surface water body comprehensive repair device as described in claim 1,3,4,5 or 6 is repaired to polluted surface water body.

8. the polluted surface water body method for comprehensive repairing of immobilized cell technology is based on as claimed in claim 7, and its feature exists In the method specifically includes following steps：

Step one, the preparation of matrix solution：

Configuration matrix solution, COD is 500mg/L in described matrix solution, and ammonia nitrogen concentration is 20mg/L, and phosphate concn is 10mg/L；

Step 2, the preparation of bacteria suspension：

The mud mixed liquid of pending polluted surface water locality Sewage Plant is taken, mixed genetic-neural network is taken in this example is 3000mg/L, by the fermentation tank of mud mixed liquid matrix solution as obtained in 30% volume ratio is inoculated in containing step one In, adding nutriment so that the concentration of the yeast extract in fermentation tank is 50mg/L, the small-scale inorganic salt in fermentation tank is (NH₄)₂SO₄、KH₂PO₄、MgSO₄、FeSO₄And CaCl₂；

The constant temperature aeration domestication at 30 DEG C, changes a water, detection COD, ammonia nitrogen and total phosphorus content, when the bacterium tamed out for every 2~3 days Plant after reaching more than 90% to the degradation capability of COD, ammonia nitrogen and total phosphorus, be enlarged culture, strain is accessed into LB culture mediums, 30 DEG C of constant temperature aeration culture 2d, 24h is staticly settled by the bacterium solution after culture in fermentation tank, and abandoning supernatant is slow with phosphate Fliud flushing is washed, and equally staticly settles rear abandoning supernatant, thalline is suspended in phosphate buffer, forms bacteria suspension, and bacterium is hanged Content of microorganisms in liquid is 20 × 10¹¹~30 × 10¹¹Individual .mL^-1；

Step 3, the embedding and fixation of microorganism：

Compound concentration is 4% sodium alginate soln, as embedded material, after outstanding with bacterium obtained in step 2 after its thoroughly dissolving Liquid uniformly mixes in equal volume.Mixed liquor is filled into inside the length about chalina of 30cm, it is 4% then to put it into concentration CaCl₂18h is crosslinked in solution.After crosslinking terminates, aseptic water washing chalina is used, obtain being adsorbed with the sponge gourd of immobilized microorganism Flesh；

Step 4, repairs：

The chalina for being adsorbed with immobilized microorganism that step 3 is obtained is filled into biological case, then by polluted surface water body synthesis Prosthetic device suspends in being put into polluted surface water body, opens aeration mechanism, is repaired.

9. the polluted surface water body method for comprehensive repairing of immobilized cell technology is based on as claimed in claim 8, and its feature exists 4 artificial aquatic weeds are hung in the lower section of, each biological case, a diameter of 5cm of described artificial aquatic weed, length is 1.5m.

10. the polluted surface water body method for comprehensive repairing of immobilized cell technology is based on as claimed in claim 8, and its feature exists In dissolved oxygen content maintains more than 4mg/L during described aeration mechanism causes device surrounding water.