CN106665436A - Method of transplanting healthy fish intestinal feces and treating ill fishes - Google Patents

Abstract

The invention relates to a method of transplanting healthy fish intestinal feces and treating ill fishes. The method comprises the steps of S1, collecting healthy fish intestinal feces, adding the collected feces into stroke-physiological saline solution for dissolution, stirring the solution evenly, filtering the solution, placing the filtered healthy feces bacteria fluid into a vessel, S2, adding filter-feeding fishes in the vessel, wherein the mass of each fish is about 50 g, the adding quantity is 10-20 fishes per cubic meter of water body; supplementing oxygen into the water to keep the filter-feeding fishes alive, breeding the filter-feeding fishes in the vessel for 10-20 days continuously, S3, adding fishes inflicted with intestinal bacterial diseases into the vessel based on the quantity of 5-10 fishes per cubic meter of water body, and breeding for 10-20 days. According to the method of transplanting healthy fish intestinal feces and treating ill fishes, the transplantation of health fish intestinal bacteria can improve intestinal microbiome of the ill fishes, the survival rate of the ill fishes are obviously increased, and therefore the method is applicable for large scale application.

Description

A method for transplanting gut feces from healthy fish to treat diseased fish

technical field

The invention relates to the field of fish farming, in particular to a method for treating diseased fish by transplanting intestinal feces of healthy fish.

Background technique

In recent years, research on human gut microbes has become a hot spot, and many studies have found that human gut microbes are closely related to human diseases, health and growth. The Human Gut Microbiome Project is considered the second genome project. Gut microbes in other animals have been less studied than those in humans.

The research on fish gut microbes is still in its infancy, and there are few studies on the correlation between the composition and species of gut microbes and the growth, nutrition, and immunity of fish itself. At present, researchers have promoted the growth of fish and improved the immunity of fish by adding nutrients, immune enhancers, and Chinese herbal medicines to fish feed. However, there is no way to improve the survival rate and treat fish with bacterial diseases by using the intestinal flora of other healthy fish to change its own intestinal flora. Because the traditional method of using intestinal bacteria is solid drug administration, and fish live in water, fish feed is put into the water, which is obviously different from the existing method, solid drug administration is not suitable for fish, and currently There is no record of liquid administration. Therefore, there is an urgent need for a method to change the intestinal flora structure of diseased fish by transplanting the intestinal feces (flora) of healthy fish into the intestinal tract of diseased fish, thereby improving the survival rate of fish and increasing its body immunity. method of force.

Contents of the invention

The purpose of the present invention is to solve the problem in the prior art that there is no method for treating diseased fish through intestinal bacteria of healthy fish, and to provide a method for treating diseased fish by transplanting intestinal feces of healthy fish .

Technical scheme of the present invention is:

The present invention has the following beneficial effects: the method for treating diseased fish of the present invention is realized by transplanting intestinal bacteria of healthy fish. Fish suffering from bacterial diseases, such as carp, grass carp, crucian carp, etc., are cultured in an environment where healthy fish and intestinal bacteria are added at the same time. After about 10-20 days, the mortality rate of the diseased fish can be found Greatly reduced. This shows that healthy fish and their intestinal bacteria fluid have played a decisive role in the recovery of diseased fish, which has improved the intestinal microbial composition of diseased fish and significantly increased their survival rate. It greatly reduces the difficulty of treating diseased fish. Moreover, the method has low cost and is suitable for large-scale popularization and application.

detailed description

A method of transplanting healthy fish intestinal feces to treat diseased fish of the present invention mainly includes the following steps:

Step 1. Collect intestinal feces from healthy fish, add them to sterile saline to dissolve, stir evenly, filter, and put the filtered healthy fecal bacteria liquid into a container;

Step 2. Add filter-feeding fish into the container, the weight of which is 50g, and the quantity is 10-20 tails/m ³ water body, oxygenate to keep the filter-feeding fish healthy and alive, and the filter-feeding fish is in the container Continue to breed for 10-20 days;

Step 3: Put the fishes suffering from intestinal bacterial diseases into the container according to the quantity of 5-10 fish/m ³ water body, and culture them for 10-20 days.

The operations in the above step 1 were all carried out in a sterile environment; the intestinal feces of healthy fish were collected by siphoning, and the ratio of fish intestinal feces to sterile saline was 1:10.

In the above-mentioned step 2, the filter-feeding fish is healthy silver carp or silver carp, the addition ratio of fish is 10-20 fish/m ³ water body, and the fish are continuously cultured in the container for about 10-20 days. The best breeding time is 15 days.

In step 3, the fishes suffering from bacterial diseases are carp, grass carp, crucian carp, etc., which are placed in the container according to 5-10 fish/ ^m3 of water body, and cultured for 10-20 days. The ratio of filter-feeding fish to fish with intestinal bacterial diseases was 2:1.

Add healthy filter-feeding fish in step 2, the microorganisms (beneficial bacteria) in the healthy fecal bacteria liquid are swallowed by the filter-feeding fish and enter the intestinal tract, continue to multiply in the intestinal tract of the healthy filter-feeding fish, and then excreted into the water body of the container , thereby increasing the number of beneficial bacteria in the water body and ensuring the activity of beneficial bacteria. The excrement of healthy filter-feeding fish also provides the nutrients needed for the survival and reproduction of beneficial bacteria in the water body, further increasing the number, concentration and vitality of beneficial bacteria in the water body. The present invention will be further described below in conjunction with specific embodiment:

Specific implementation mode one

The method of the present invention comprises the following steps: step 1, utilize the method for siphon, fish excrement is collected in the aseptic container, add aseptic physiological saline to dissolve it, the ratio of fish intestinal excrement and sterile water is 1: 10. Filter the dissolved fish intestinal feces mixture through sterile ordinary qualitative filter paper to filter out solid residues. The filtered liquid is placed in a pre-sterilized open square tank. The square tank depends on the specific fish. The size setting; the amount of bacterial solution added to the square tank depends on the size of the fish that needs to be put in, and it is enough to cover the fish;

Step 2: Add filter-feeding silver carp or silver carp into the square tank at a ratio of 10-20 fish/ ^m3 of water body, oxygenate to keep the silver carp healthy and alive, and keep the filter-feeding fish in the container for 10-20 sky;

Step 3: Carp, grass carp, crucian carp, etc. suffering from bacterial diseases are placed in a square tank according to 5-10 fish/m ³ water body, and cultured for 10-20 days.

Specific implementation mode two

The implementation method includes the following steps: Step 1, which is the same as the specific embodiment 1;

Step 2, placing carp, grass carp and crucian carp suffering from bacterial diseases in a square tank according to 5-10 fish/m ³ water body, and cultivating them for 10-20 days.

Specific implementation mode three

The implementation method comprises the following steps: step 1, select a square tank with the same size as that in the specific embodiment one, add filter-feeding silver carp or silver carp, the addition ratio is 10～20 tails/m ³ water body, oxygenate to keep silver carp The fish is healthy and alive, and the filter-feeding fish is continuously cultured in the container for 10-20 days;

Step 2, placing carp, grass carp and crucian carp suffering from bacterial diseases in a square tank according to 5-10 fish/m ³ water body, and cultivating them for 10-20 days.

Specific implementation mode four

The implementation method includes the following steps: select a square tank with the same size as that in the specific embodiment one, add carp, grass carp, and crucian carp suffering from bacterial diseases into the square tank according to 5-10 tails/ ^m3 water body, and breed 10-20 sky.

Experimental effect

The present invention adopts following experimental verification effect:

Select 480 diseased carps, divide them into 4 groups, operate according to the method of the above-mentioned specific embodiment 1 to 4 respectively, make 3 parallel control groups for each group, and place 40 fish in each control group. See Table 1 below for specific groupings.

Table 1

After about 15 days of breeding, it can be found that the diseased fish in group 1 died the least, and the diseased fish began to recover. The effect of adding healthy bacteria to group 2 was similar to that of adding silver carp to group 3, but it was significantly lower than that of group 3. 1, while in group 4 without supplementation, silver carp died in large numbers.

This shows that in the same breeding environment, adding healthy fish and their intestinal bacteria at the same time played a decisive role in the recovery of diseased fish. If only the bacterial solution of healthy fish is added, the concentration of the bacterial solution will gradually decrease as time increases, and the therapeutic effect will also decrease. If only healthy fish is added, the concentration of its excreted feces in the water is always low, and a good therapeutic effect cannot be achieved. In the present invention, the two are mixed and added into water, and on the basis of the added bacterial liquid concentration, the concentration of the bacterial liquid is kept at a relatively high level through the continuous defecation of healthy fish, and will not decrease with time, so that the sick The intestinal microbial composition of the fish has been fundamentally improved, its survival rate has been significantly improved, and the difficulty of treating diseased fish has been greatly reduced.

The above content is only a preferred embodiment of the present invention, and is not intended to limit the implementation of the present invention. Those of ordinary skill in the art can easily make corresponding modifications or modifications according to the main concept and spirit of the present invention. Therefore, the present invention The scope of protection should be subject to the scope of protection required by the claims.

Claims (9)

1. it is a kind of to transplant healthy method of the fish enteron stool to treat ill fish, it is characterised in that to comprise the steps：

Step one, the healthy fish intestines feces of collection, dissolve in being added to physiological saline solution, filter after stirring, will filter Healthy feces bacterium solution afterwards is placed in container；

Step 2, filter-feeding fish is added in the above-described container, addition quantity is 10~20 tails/m³Water body, oxygenation are eaten with keeping filter Property healthy fish survival, filter-feeding fish in the above-described container persistently cultivate 10~20 days；

Step 3, will suffer from intestinal bacteriosis Fish according to 5~10 tails/m³Quantity be placed in the container, cultivation 15 ~20 days.

2. it is according to claim 1 to transplant healthy method of the fish enteron stool to treat ill fish, it is characterised in that described In step one, filter-feeding fish is spotted silver carp or Hypophthalmichthys molitrix.

3. it is according to claim 1 to transplant healthy method of the fish enteron stool to treat ill fish, it is characterised in that step The ratio of one Mesichthyes enteron stool and physiological saline solution is 1:10.

4. it is according to claim 1 to transplant healthy method of the fish enteron stool to treat ill fish, it is characterised in that step Method of the healthy fish intestines feces using siphon is collected in one.

5. it is according to claim 1 to transplant healthy method of the fish enteron stool to treat ill fish, it is characterised in that step Operation in one is carried out in an aseptic environment.

6. it is according to claim 1 to transplant healthy method of the fish enteron stool to treat ill fish, it is characterised in that step In two, the additional proportion of filter-feeding fish is 10~20 tails/m³Water body, filter-feeding fish persistently cultivate 10~20 in a reservoir My god.

7. it is according to claim 1 to transplant healthy method of the fish enteron stool to treat ill fish, it is characterised in that step Ill Fish in three should be the Cyprinus carpio with bacterial disease, Ctenopharyngodon idellus or Carassius auratuss.

8. it is according to claim 1 to transplant healthy method of the fish enteron stool to treat ill fish, it is characterised in that step The Fish with bacterial disease in three are according to 5~10 tails/m³Water body is placed in the container, is cultivated 10~20 days.

9. it is according to claim 1 to transplant healthy method of the fish enteron stool to treat ill fish, it is characterised in that filter food Property fish to throw in mantissa ratio with the fish with intestinal bacteriosis be 2:1.