CN106566857A - Process for producing yeast protein from peel residues generated in whole potato flour production process - Google Patents
Process for producing yeast protein from peel residues generated in whole potato flour production process Download PDFInfo
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- CN106566857A CN106566857A CN201610936499.6A CN201610936499A CN106566857A CN 106566857 A CN106566857 A CN 106566857A CN 201610936499 A CN201610936499 A CN 201610936499A CN 106566857 A CN106566857 A CN 106566857A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
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Abstract
The invention discloses a process for producing a yeast protein from peel residues generated in a whole potato flour production process. The process comprises the steps of degrading amylose and amylopectin in the potato peel residues into dextrin and various low molecular sugars under the condition of proper temperature, pH and oxygen content by adopting mixed bacteria; degrading cellulose and hemicellulose in the potato peel residues into glucose; and further converting the glucose, and a nitrogen source and inorganic salt externally added to a culture medium into the yeast protein. The yeast protein is produced through microbial fermentation; the yeast protein is rich in nutritional components; the protein content is 45-55%; and the yeast protein is complete in amino acid components, contains various vitamins, minerals, organic acids and bio-active substances, can be widely used in food processing and feed, and has a wide development prospect.
Description
Technical field
The present invention relates to a kind of method of Rhizoma Solani tuber osi yeast protein, using producing in potato full-powder production process
Skin slag produces the process of yeast protein.
Background technology
Potato full-powder is liked by people rich in multivitamin and mineral etc., city because of its low fat, low sugar point
Field is also increasing to the demand of full powder, thus Rhizoma Solani tuber osi is defined as the 4th staple food grain by our countries.Peel of Rhizoma Solani tuber osi slag is
Main By product in full powder production process, main component is starch, cellulose, hemicellulose, pectin and a small amount of protein,
Its water content is big, rich in nutrition, is difficult to store, transports, easily putrid and deteriorated, stench, forms the wasting of resources and environmental pollution.How
The economic benefit that economy, low power consuming process peel of Rhizoma Solani tuber osi slag and produced is that potato full-powder produces faced a great class
Topic, is the major issue for being related to national economy.
The content of the invention
The present invention using mixed bacteria under conditions of suitable temperature, pH, oxygen content by the straight chain in peel of Rhizoma Solani tuber osi slag,
Amylopectin is degraded to dextrin and various low molecule saccharides, while by the cellulose in peel of Rhizoma Solani tuber osi slag, hemicellulose degradation being
Glucose, is further together converted into yeast protein with nitrogen source additional in culture medium, inorganic salt.
The technical scheme is that what is be achieved in that：The skin slag production that a kind of utilization potato full-powder production process is produced
The technique of yeast protein, carries out micronizing process to peel of Rhizoma Solani tuber osi slag, adds the fermentation tank enzymolysis after sterilizing, reason be due to
Yeast can not directly by the use of the starch in skin slag as carbon source, it is therefore desirable to the starch in potato slag is carried out enzymolysis and is converted into Fructus Vitis viniferae
Sugar.As in peel of Rhizoma Solani tuber osi slag, itself nitrogen substance is less, crude fiber content is higher, it is impossible to fully meet needed for the growth of yeast
Must also additional other nutritional labelings, add carbamide and potassium dihydrogen phosphate after enzymolysis and supplement yeast desired nutritional composition.Then adjust
PH to 6.0-6.5, adds aspergillus oryzae and yeast, inoculum concentration less than or equal to fermentation dry matter content 0.2%, during fermentation culture
Temperature 30-32 DEG C, fermenter base are passed through filtrated air, ventilating ratio 0.5:1-1:1, when in fermentation liquid, residual sugar has consumed, fermentation knot
Beam, the product dehydration fermented, drying obtain yeast protein.After yeast fermentation, in skin slag, the water of combined state is by microorganism
The water of free state is decomposed into, material component layer state, upper strata are thalline yeast, and lower floor is water, is that heat disappears when reducing drying
Consumption is first to drying after material centrifugal dehydration treatment again.
Further, first aspergillus oryzae is accessed in fermentation tank and is cultivated, cultivation temperature 29-31 DEG C, ventilating ratio 0.5:1, fermentation
Yeast is accessed into fermentation tank, cultivation temperature again when aspergillus oryzae amount reproduction enters exponential phase in fermentation tank to during 24h
For 29-32 DEG C, ventilating ratio 1:1, it is further cultured for 24h.
Further, there is the reasons such as the big, poor fluidity of block and cause in sweat at microorganism in peel of Rhizoma Solani tuber osi slag
Reason is difficult, and conversion ratio is low, while material physicochemical property levies shakiness brings inconvenience to detection, it is necessary to peel of Rhizoma Solani tuber osi slag elder generation
Carry out Mechanical Crushing.Carry out micronizing process after pulverization process again, particulate matter diameter is less than 1 ㎜ after micronizing process, makes
Potato cell wall construction is degraded, and skin slag Hydrophilic Fiber strengthens, and the viscosity of system, uniformity are significantly improved.
Further, the charge weight of peel of Rhizoma Solani tuber osi slag is less than the 70% of fermenter volume.
Further, the aspergillus oryzae and yeast are suspensions, and its access amount is the 1% of fermented product volume.
Further, the amount of addition carbamide and potassium dihydrogen phosphate is the 0.05-1.0% of fermentation dry matter content
Beneficial effects of the present invention are：Can be led to as the substrate of micro-organisms by the use of the nutritional labeling that potato residue is rich in
Cross fermentable production yeast protein, yeast protein rich in nutrition content, protein content between 45%～55%, aminoacid group
Divide complete, containing multivitamin, mineral, organic acid, bioactive substance, can be widely applied to food processing and feedstuff
In, with vast potential for future development.
Specific embodiment
Explanation is further explained to the present invention with reference to specific embodiment.
1st, the preparation of seed, sterilizing, inoculation, fermentation
The first class seed pot that peel of Rhizoma Solani tuber osi slag is entered after sterilizing after colloid mill micronizing, charge weight for volume 70%,
Then skin slag is digested, after enzymolysis, adds carbamide and potassium dihydrogen phosphate, proportion is respectively 0.5%, 0.05%, then uses high concentration
Alkali liquor adjust pH to 6.5, then material carry out it is real disappear, 121-123 DEG C of the temperature that disappears in fact is maintained 30 minutes, and being cooled to 32 DEG C will
Aspergillus oryzae accesses first class seed pot culture, inoculum concentration for fermented product volume 1%, 29～31 DEG C of cultivation temperature, fermentation tank in culture
Bottom is passed through filtrated air, and tank pressure maintains 0.05MPa, ventilating ratio 0.5:1,120 r/min of speed of agitator, cultivate to during 24h and work as
Again yeast is intervened in the seed tank when in fermentation tank, aspergillus oryzae amount reproduction enters exponential phase, cultivation temperature 29～32
DEG C, tank pressure maintains 0.05MPa, ventilating ratio 1:Material is all accessed after being further cultured for about 24h real by 1,150 r/min of speed of agitator
Eliminate the secondary seed tank after bacterium, coefficient 70%, ventilating ratio 1:1, other conditions are constant, by thing after continuing to cultivate 24 hours
The whole airlift fermentors accessed after eliminating bacterium in fact of material, coefficient 70%, condition of culture is constant, and fermentation is extremely fermented for 24 hours
Liquid residual sugar has consumed, and fermentation ends put tank.
2nd, the centrifugation of product, drying
Product to having fermented first passes through high speed centrifuge processed, material high temperature is dried using roller dryer after process
It is dry, then it is grinding to obtain finished product yeast protein.
Yeast produces the impact of single cell protein to peel of Rhizoma Solani tuber osi slag（It is shown in Table one）, yeast protein product percentage composition is
70.23%, illustrate to cause thalline Repiration to consume substantial amounts of nutrient substance due to fermentation, so as to obtain dry relatively
Low, the reducing sugar in fermentation liquid causes to consume aggravation so as to content is reduced due to the increase of thalline quantity, and protein content is substantially carried
Height, reaches 46.12%.
One conversion of saccharomycetes potato residue of table produces the impact of single cell protein
| Strain | Mass percent（%） | Solution reduction sugar（OD) | With respect to thalline quantity (%) | Protein content (%) |
| Yeast | 70.23 | 0.047 | 68.34 | 46.12 |
| Blank | 100 | 0.593 | 16 | 4.6 |
The present invention effectively degrades the cellulose in peel of Rhizoma Solani tuber osi slag, and protein content, wherein amino acid composition greatly improved
It is complete, it is necessary to which that amino acid content is higher, while being rich in organic acid, vitamin, inorganic salt, bioactie agent, biological value
Better than vegetable protein, can be widely applied in food processing and feedstuff.And show that animal growth state is good by zoopery
And obtain obvious gaining effect.Rhizoma Solani tuber osi Yeast protein feed is added to into replacement part bean cake in rat feed（Yeast egg
White group）, with peel of Rhizoma Solani tuber osi slag oleo stock（Oleo stock group）With the feedstuff for being not added with yeast protein（Matched group）For control, Rhizoma Solani tuber osi is investigated
Impact of the yeast protein to Growth in Rats, experiment show the rat weightening of the forage feed for adding yeast protein apparently higher than not adding
Plus the rat that the feedstuff and oleo stock of yeast protein is fed（It is shown in Table two）.
Two Yeast protein feed results of animal of table is compareed
| Group | Head number | Head starting weight（Gram） | Head end weight（Gram） | Head increases weight（Gram） | Average daily gain（Gram） |
| Matched group | 8 | 56.06±3.02 | 216.22±8.07 | 160.16±8.78 | 5.72±0.32 |
| Oleo stock group | 8 | 56.09±2.88 | 209.46±10.12 | 153.37±6.04 | 5.47±0.24 |
| Yeast protein group | 8 | 56.14±4.10 | 228.67±12.23 | 172.53±10.22 | 6.16±0.38 |
The above, the only present invention preferably specific embodiment, but protection scope of the present invention is not limited thereto, any
Those familiar with the art in the technical scope of present disclosure, technology according to the present invention scheme and its invention
Design in addition equivalent or change, should all be included within the scope of the present invention.
Claims (6)
1. the technique that the skin slag that a kind of utilization potato full-powder production process is produced produces yeast protein, it is characterised in that to horse
Bell potato skin slag carries out micronizing process, adds the fermentation tank enzymolysis after sterilizing, adds carbamide and potassium dihydrogen phosphate, adjust after enzymolysis
Section pH to 6.0-6.5, adds aspergillus oryzae and yeast, and temperature 30-32 DEG C during fermentation culture, fermenter base are passed through aseptic sky
Gas, ventilating ratio 0.5:1-1:1, when in fermentation liquid, residual sugar has consumed, fermentation ends, the product dehydration fermented, drying obtain yeast
Albumen.
2. the skin slag that utilization potato full-powder production process according to claim 1 is produced produces the technique of yeast protein,
Characterized in that, first aspergillus oryzae is accessed in fermentation tank cultivating, cultivation temperature 29-31 DEG C, ventilating ratio 0.5:1, when fermenting to 24h
Yeast is accessed into fermentation tank again when aspergillus oryzae amount reproduction enters exponential phase in fermentation tank, cultivation temperature is 29-32
DEG C, ventilating ratio 1:1, it is further cultured for 24h.
3. the skin slag that utilization potato full-powder production process according to claim 1 is produced produces the technique of yeast protein,
Characterized in that, first mechanical activation comminution is carried out to Rhizoma Solani tuber osi, then carry out micronizing process, the particle diameter after micronizing process is little
In equal to 1 ㎜.
4. the skin slag that utilization potato full-powder production process according to claim 1 is produced produces the technique of yeast protein,
Characterized in that, the charge weight of peel of Rhizoma Solani tuber osi slag is less than the 70% of fermenter volume.
5. the skin slag that utilization potato full-powder production process according to claim 1 and 2 is produced produces the work of yeast protein
Skill, it is characterised in that the aspergillus oryzae and yeast are high-concentration bacterial suspensions, its access amount is the 1% of fermented product volume.
6. the skin slag that utilization potato full-powder production process according to claim 1 is produced produces the technique of yeast protein,
Characterized in that, the amount of addition carbamide and potassium dihydrogen phosphate is the 0.05-1.0% of fermentation dry matter content.
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| CN201610936499.6A CN106566857A (en) | 2016-11-01 | 2016-11-01 | Process for producing yeast protein from peel residues generated in whole potato flour production process |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110892986A (en) * | 2019-12-26 | 2020-03-20 | 福建农林大学 | High-vitamin C potato enzyme powder |
| CN114072007A (en) * | 2019-05-24 | 2022-02-18 | 艾维贝皇家合作公司 | Proteins from peeled tubers |
Citations (5)
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| CN101283730A (en) * | 2008-04-30 | 2008-10-15 | 杨谦 | Technological process for producing the single-cell protein by liquid state fermentation of potato marc |
| CN102687796A (en) * | 2012-06-06 | 2012-09-26 | 新疆维吾尔自治区畜牧科学院饲料研究所 | Method for producing bioprotein feed using tomato pomace |
| CN103156051A (en) * | 2013-01-16 | 2013-06-19 | 上海清美绿色食品有限公司 | Method using composite bacterium to ferment bean dregs to manufacture protein feed |
| CN103652328A (en) * | 2013-12-13 | 2014-03-26 | 广西科技大学 | Method for producing high-quality high-protein feed through mixed strain solid-state fermentation of rice dregs and manioc waste |
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2016
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Patent Citations (5)
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| CN101283730A (en) * | 2008-04-30 | 2008-10-15 | 杨谦 | Technological process for producing the single-cell protein by liquid state fermentation of potato marc |
| CN102687796A (en) * | 2012-06-06 | 2012-09-26 | 新疆维吾尔自治区畜牧科学院饲料研究所 | Method for producing bioprotein feed using tomato pomace |
| CN103156051A (en) * | 2013-01-16 | 2013-06-19 | 上海清美绿色食品有限公司 | Method using composite bacterium to ferment bean dregs to manufacture protein feed |
| CN103652328A (en) * | 2013-12-13 | 2014-03-26 | 广西科技大学 | Method for producing high-quality high-protein feed through mixed strain solid-state fermentation of rice dregs and manioc waste |
| CN104920806A (en) * | 2015-07-06 | 2015-09-23 | 青岛嘉瑞生物技术有限公司 | Bran protein feed preparing method by using mixed bacteria multi-step fermentation |
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| 余顺火主编: "《食品（生物）生产实习教程》", 31 July 2016 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114072007A (en) * | 2019-05-24 | 2022-02-18 | 艾维贝皇家合作公司 | Proteins from peeled tubers |
| CN110892986A (en) * | 2019-12-26 | 2020-03-20 | 福建农林大学 | High-vitamin C potato enzyme powder |
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