CN106511236A - Skin regeneration promotion and ageing prevention Dendrobium huoshanense preparation - Google Patents
Skin regeneration promotion and ageing prevention Dendrobium huoshanense preparation Download PDFInfo
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- CN106511236A CN106511236A CN201611057062.1A CN201611057062A CN106511236A CN 106511236 A CN106511236 A CN 106511236A CN 201611057062 A CN201611057062 A CN 201611057062A CN 106511236 A CN106511236 A CN 106511236A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 18
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- 241001678082 Dendrobium huoshanense Species 0.000 title abstract 6
- 230000002265 prevention Effects 0.000 title abstract 4
- 239000000284 extract Substances 0.000 claims abstract description 46
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- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 claims abstract description 6
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/36—Carboxylic acids; Salts or anhydrides thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Dermatology (AREA)
- Emergency Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a skin regeneration promotion and ageing prevention Dendrobium huoshanense preparation. The skin regeneration promotion and ageing prevention Dendrobium huoshanense preparation is prepared from, by weight, 10-15 parts of Dendrobium huoshanense freeze-dried powder, 10-20 parts of olive fruit extract, 10-15 parts of Helix Aspersa Muller secretion, 10-20 parts of wall-broken pine pollen, 5-8 parts of Folium Ginkgo extract, 15-25 parts of Salvia miltiorrhiza micro-powder, 3-5 parts of Binchotan charcoal micro-powder, 2-3 parts of epidermal growth factor, 3-5 parts of Fructus Foeniculi powder, 1-3 parts of mandelic acid, 1-3 parts of beta-glucan, 5-9 parts of Lycium ruthenicum Murr extract, 10-15 parts of green plum yeast powder, 5-10 parts of ginseng and 5-10 parts of Radix Astragali. The skin regeneration promotion and ageing prevention Dendrobium huoshanense preparation prepared by adopting the self-made Dendrobium huoshanense freeze-dried powder and olive fruit extract as main components and adding the Folium Ginkgo extract, wall-broken pine pollen and the green plum yeast powder has very substantial skin regeneration promoting and cell ageing delaying effects.
Description
Technical field
The present invention relates to field of health care products, and in particular to a kind of Herba Dendrobii promotes skin regeneration combination anti-senile preparation.
Background technology
Nowadays what modern faced is not only the pressure of life, and operating pressure is also increasing.Skin is to be covered in
The maximum organ of human body surface, due to directly being contacted with external environment, is aoxidized, a variety of tension-causing factors such as dry and ultraviolet
Stimulation, therefore be highly susceptible to damage.Meanwhile, and because the impact of the internal factor such as age, diet and living habit, causes
The decline of skin function and hereditary abnormalization, so that skin is more prone to aging.As skin is the outermost in human body,
U.S. of human body is demonstrated by a glance.So, anti-aging cosmetics into vast female consumer urgent needss product it
One.
Anti-aging cosmetics have accounted for one of more than the 50% of Cosmetic Market, topmost factor of skin aging, are skins
The skin photoage that skin is caused by ultraviolet radioactive for a long time.The photoaging of skin is that free radical can as in skin, free radical increases
Damage biological film, promotes elastin fibers to increase and amorphization, and collagen fabric is substantially reduced and Fructus Vitis viniferae ammonia
The skin ageing phenomenons such as polysaccharide increase.Many women in order to firm skin, it is anti-ageing wait for a long time, do not stint the urgent cause pin in beauty parlor, but
It is that the situation of skin but go fromes bad to worse, or even produces insoluble severe allergy phenomenon.Therefore a kind of safety of exploitation is needed to have
The cosmetics of the firm skin defying age of effect.
Herba Dendrobii, also known as rice dry measure used in former times, is the superfine product in Herba Dendrobii, is the endangered rare medical material of China national.Its drying
Stem (Huo Fengdou) and fresh dry measure used in former times can be used as medicine.
《Compendium of Materia Medica》Record：Herba Dendrobii is sweet flat nontoxic.Damaging the spleen and stomach, the eliminating impediment therapeutic method to keep the adverse QI flowing downwards are cured mainly, tonifying five ZANG-organses asthenia wins thin, reinforcing YIN-essence benefit
Essence, long term usage thickness the intestines and stomach, never sufficient in benefit, flat stomach gas, long muscle, by skin pathogenic heat miliaria gas, it is weak that foot knee joint aches cold numbness.Sedate removes frightened,
Make light of one's life by commiting suicide macrobiosis, QI invigorating heat extraction, it is weak to control man's waist foot, is good for sun.By skin migratory arthralgia, cold long in bone, the kidney invigorating benefit power, strengthening bone and muscle are warmed up
Water is dirty, Fructus Alpiniae Oxyphyllae clearing heat in QI system, controls plug in heating spontaneous perspiration, carbuncle evacuation of pus.
Dry measure used in former times is rich in more than ten kinds of alkaloids such as polysaccharide, aminoacid and dendrobine, Herba Dendrobii amine bases suddenly.Jing Chinese Academy of Medical Sciences medicine
Identified with Institute of Zoology：Polyoses content is 18.974%, and total alkaloid content is 0.030%.Immunity of organism can be effectively improved
Function, to human body eye, pharynx, lung.The disease such as the organs such as stomach, intestinal, kidney and blood, cardiovascular has special efficacy.Energy anti-cataract, prolongs
Slow aging, mutation, antitumor.
The content of the invention
To solve the above problems, the invention provides a kind of Herba Dendrobii promotes skin regeneration combination anti-senile preparation, with
Composition, collaboration Catalpa leaf extract, breaking cellular wall Pollen Pini, green grass or young crops are wanted based on homemade Herba Dendrobii lyophilized powder and Fructus Canarii albi berry extract
The addition of prunus mume (sieb.) sieb.et zucc. yeast powder etc., promotion skin regeneration and the effect for delaying cell senescence of highly significant.
For achieving the above object, the technical scheme taken of the present invention is：
A kind of Herba Dendrobii promotes skin regeneration combination anti-senile preparation, is prepared from by the raw material of following weight portion：
Herba Dendrobii lyophilized powder 10-15 parts, Fructus Canarii albi berry extract 10-20 parts, spiral Limax juice 10-15 parts, breaking cellular wall
Spiral shell Pollen Pini 10-20 parts, Catalpa leaf extract 5-8 parts, Rhizoma Seu Herba Bergeniae micropowder 15-25 parts, Binchotan micropowder 3-5 parts, epidermal growth
Factor 2-3 part, fennel powder 3-5 part, mandelic acid 1-3 parts, beta glucan 1-3 parts, Lycium ruthenicum Murr. extract 5-9 parts, Armeniaca mume Sieb. yeast powder
10-15 parts, Radix Ginseng 5-10 parts, Radix Astragali 5-10 parts.
Preferably, the Herba Dendrobii lyophilized powder is as obtained by prepared by following steps：
S1, the Herba Dendrobii that drains will be cleaned it is placed in steam-explosion jar, it is 0.6- to be first passed through nitrogen to steam explosion pressure inside the tank
1.4MPa, explosion treatment 8-25min；Then it is 1.4-1.8MPa to be passed through rapidly steam to steam explosion pressure inside the tank, at steam explosion
After reason 0.5-2.5min, room temperature is concentrated into relative density 1.10-1.20, is spray-dried concentrated solution, obtains powder body；
S2, in powder body add 3-8 times of water, by mass percentage be 2-3% ratio add neutral protease, by quality
Percentage ratio is that 1-2% adds flavor protease, digests 1-2h, filter, obtain enzymolysis solution at 30-50 DEG C；
S3, by naturally to thaw again after the fermentation liquid quick freeze of gained, make age of starch precipitation in extract；Collect supernatant
Liquid, remainder centrifugation are collected centrifugal liquid and are incorporated in supernatant；
S4, by supernatant with the flow velocity of 3-7BV/h by macroporous resin column, after dynamic adsorption saturation, deionized water with
The above-mentioned macroporous resin column of flow velocity drip washing of 5-8BV/h is colourless to effluent, then with the organic solvent that volume fraction is 50%-70%
Eluting is carried out with the flow velocity of 8-12BV/h, eluent is collected, lyophilization obtains Herba Dendrobii lyophilized powder.
Preferably, the Fructus Canarii albi berry extract is as obtained by prepared by following steps：
The Fructus Canarii albi berry extract cleaned is placed in into 90% methanol or ethanol solution that flash extracter adds 6 times of volumes, place
Reason 1-3 minutes, after solid-liquid separation, reclaim methanol or ethanol solution, concentration so that extract is in paste, then with water saturation just
Butanol is extracted 3 times to extract, after recycling design, by drying extract concentrated in vacuo, obtains Fructus Canarii albi berry extract.
Preferably, the Catalpa leaf extract is as obtained by prepared by following steps：
S1, by clean Folium Ginkgo be placed in flash extracter process after, by mass percentage be 3-5% add complex enzyme hydrolysis
Liquid, digests 1-2 hours by 20-30 DEG C, and per half an hour, once, control pH value is between 3.5-6.2 for stirring, oxygenation；
Enzymolysis solution is warming up to 85-100 DEG C after terminating by S2, enzymolysis, enzyme denaturing 30-40 minutes, is filtered, and get Sheng Mali Jis are extracted
Liquid.
Preferably, the Armeniaca mume Sieb. yeast powder is as obtained by prepared by following steps：
Take the Armeniaca mume Sieb. cleaned after draining to be placed in steam-explosion jar, it is 0.7-1.5MPa to be first passed through nitrogen to steam explosion pressure inside the tank,
Explosion treatment 7-23min；Then it is 1.5-2.1MPa, Steam explosion treatment 0.8- to be passed through rapidly steam to steam explosion pressure inside the tank
After 2.8min, according to the ratio microbe inoculation leaven of inoculum concentration 1.5-1.7%, keeping temperature 25-35 DEG C, ferment 48-54
After hour, high temperature sterilize obtains Armeniaca mume Sieb. yeast powder.
Preferably, the microbe leaven is by lactobacilluss, Bacillus licheniformis, Corynebacterium glutamicum in mass ratio 3:
2:1 mixing gained.
Preferably, component of the complex enzyme hydrolysis liquid comprising following weight portion：Neutral protease 3-5 parts, flavor protease
2-5 parts.
The invention has the advantages that：
Composition is wanted based on homemade Herba Dendrobii lyophilized powder and Fructus Canarii albi berry extract, Catalpa leaf extract is cooperateed with, is broken
The addition of wall Pollen Pini, Armeniaca mume Sieb. yeast powder etc., promotion skin regeneration and the effect for delaying cell senescence of highly significant.
Specific embodiment
In order that objects and advantages of the present invention become more apparent, with reference to embodiments the present invention is carried out further
Describe in detail.It should be appreciated that specific embodiment described herein is not used to limit this only to explain the present invention
It is bright.
Following examples, the Herba Dendrobii lyophilized powder for being used is as obtained by prepared by following steps：
S1, the Herba Dendrobii that drains will be cleaned it is placed in steam-explosion jar, it is 0.6- to be first passed through nitrogen to steam explosion pressure inside the tank
1.4MPa, explosion treatment 8-25min；Then it is 1.4-1.8MPa to be passed through rapidly steam to steam explosion pressure inside the tank, at steam explosion
After reason 0.5-2.5min, room temperature is concentrated into relative density 1.10-1.20, is spray-dried concentrated solution, obtains powder body；
S2, in powder body add 3-8 times of water, by mass percentage be 2-3% ratio add neutral protease, by quality
Percentage ratio is that 1-2% adds flavor protease, digests 1-2h, filter, obtain enzymolysis solution at 30-50 DEG C；
S3, by naturally to thaw again after the fermentation liquid quick freeze of gained, make age of starch precipitation in extract；Collect supernatant
Liquid, remainder centrifugation are collected centrifugal liquid and are incorporated in supernatant；
S4, by supernatant with the flow velocity of 3-7BV/h by macroporous resin column, after dynamic adsorption saturation, deionized water with
The above-mentioned macroporous resin column of flow velocity drip washing of 5-8BV/h is colourless to effluent, then with the organic solvent that volume fraction is 50%-70%
Eluting is carried out with the flow velocity of 8-12BV/h, eluent is collected, lyophilization obtains Herba Dendrobii lyophilized powder.
The Fructus Canarii albi berry extract for being used is as obtained by prepared by following steps：
The Fructus Canarii albi berry extract cleaned is placed in into 90% methanol or ethanol solution that flash extracter adds 6 times of volumes, place
Reason 1-3 minutes, after solid-liquid separation, reclaim methanol or ethanol solution, concentration so that extract is in paste, then with water saturation just
Butanol is extracted 3 times to extract, after recycling design, by drying extract concentrated in vacuo, obtains Fructus Canarii albi berry extract.
The Catalpa leaf extract for being used is as obtained by prepared by following steps：
S1, by clean Folium Ginkgo be placed in flash extracter process after, by mass percentage be 3-5% add complex enzyme hydrolysis
Liquid, digests 1-2 hours by 20-30 DEG C, and per half an hour, once, control pH value is between 3.5-6.2 for stirring, oxygenation；
Enzymolysis solution is warming up to 85-100 DEG C after terminating by S2, enzymolysis, enzyme denaturing 30-40 minutes, is filtered, and get Sheng Mali Jis are extracted
Liquid.
The Armeniaca mume Sieb. yeast powder for being used is as obtained by prepared by following steps：
Take the Armeniaca mume Sieb. cleaned after draining to be placed in steam-explosion jar, it is 0.7-1.5MPa to be first passed through nitrogen to steam explosion pressure inside the tank,
Explosion treatment 7-23min；Then it is 1.5-2.1MPa, Steam explosion treatment 0.8- to be passed through rapidly steam to steam explosion pressure inside the tank
After 2.8min, according to the ratio microbe inoculation leaven of inoculum concentration 1.5-1.7%, keeping temperature 25-35 DEG C, ferment 48-54
After hour, high temperature sterilize obtains Armeniaca mume Sieb. yeast powder, and the microbe leaven is by lactobacilluss, Bacillus licheniformis, glutamic acid rod
Bacillus in mass ratio 3:2:1 mixing gained, component of the complex enzyme hydrolysis liquid comprising following weight portion：Neutral protease 3-5 parts,
Flavor protease 2-5 parts.
The Rhizoma Seu Herba Bergeniae micropowder is that, after Rhizoma Seu Herba Bergeniae is cleaned obtained by micronizing, Binchotan micropowder is obtained by Binchotan micronizing；
Turpentine derivatives of the organic solvent for natural origin.Isomery, disproportionation products of the turpentine derivatives for pinene, its
Its raw material is commercially available.
Embodiment 1
A kind of Herba Dendrobii promotes skin regeneration combination anti-senile preparation, is prepared from by the raw material of following weight portion：
10 parts of Herba Dendrobii lyophilized powder, 10 parts of Fructus Canarii albi berry extract, 10 parts of spiral Limax juice, breaking cellular wall spiral shell Pollen Pini 10
Part, 5 parts of Catalpa leaf extract, 15 parts of Rhizoma Seu Herba Bergeniae micropowder, 3 parts of Binchotan micropowder, 2 parts of epidermal growth factor, 3 parts of fennel powder,
1 part of mandelic acid, 1 part of beta glucan, 5 parts of Lycium ruthenicum Murr. extract, 10 parts of Armeniaca mume Sieb. yeast powder, 5 parts of Radix Ginseng, 5 parts of the Radix Astragali.
Embodiment 2
A kind of Herba Dendrobii promotes skin regeneration combination anti-senile preparation, is prepared from by the raw material of following weight portion：
15 parts of Herba Dendrobii lyophilized powder, 20 parts of Fructus Canarii albi berry extract, 15 parts of spiral Limax juice, breaking cellular wall spiral shell Pollen Pini 20
Part, 8 parts of Catalpa leaf extract, 25 parts of Rhizoma Seu Herba Bergeniae micropowder, 5 parts of Binchotan micropowder, 3 parts of epidermal growth factor, 5 parts of fennel powder,
3 parts of mandelic acid, 3 parts of beta glucan, 9 parts of Lycium ruthenicum Murr. extract, 15 parts of Armeniaca mume Sieb. yeast powder, 10 parts of Radix Ginseng, 10 parts of the Radix Astragali.
Embodiment 3
A kind of Herba Dendrobii promotes skin regeneration combination anti-senile preparation, is prepared from by the raw material of following weight portion：
12.5 parts of Herba Dendrobii lyophilized powder, 15 parts of Fructus Canarii albi berry extract, 12.5 parts of spiral Limax juice, breaking cellular wall spiral shell Flos pini
15 parts of powder, 6.5 parts of Catalpa leaf extract, 20 parts of Rhizoma Seu Herba Bergeniae micropowder, 4 parts of Binchotan micropowder, 2.5 parts of epidermal growth factor, Fructus Foeniculi
4 parts of XIANGFEN, 2 parts of mandelic acid, 2 parts of beta glucan, 7 parts of Lycium ruthenicum Murr. extract, 12.5 parts of Armeniaca mume Sieb. yeast powder, 7.5 parts of Radix Ginseng, the Radix Astragali
7.5 part.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (7)
1. a kind of Herba Dendrobii promotes skin regeneration combination anti-senile preparation, it is characterised in that by the raw material system of following weight portion
It is standby to form：
Herba Dendrobii lyophilized powder 10-15 parts, Fructus Canarii albi berry extract 10-20 parts, spiral Limax juice 10-15 parts, breaking cellular wall spiral shell pine
Pollen 10-20 parts, Catalpa leaf extract 5-8 parts, Rhizoma Seu Herba Bergeniae micropowder 15-25 parts, Binchotan micropowder 3-5 parts, epidermal growth factor
2-3 parts, fennel powder 3-5 part, mandelic acid 1-3 parts, beta glucan 1-3 parts, Lycium ruthenicum Murr. extract 5-9 parts, Armeniaca mume Sieb. yeast powder 10-
15 parts, Radix Ginseng 5-10 parts, Radix Astragali 5-10 parts.
2. a kind of Herba Dendrobii as claimed in claim 1 promotes skin regeneration combination anti-senile preparation, it is characterised in that described
Herba Dendrobii lyophilized powder is as obtained by prepared by following steps：
S1, the Herba Dendrobii that drains will be cleaned it is placed in steam-explosion jar, it is 0.6-1.4MPa to be first passed through nitrogen to steam explosion pressure inside the tank,
Explosion treatment 8-25min；Then it is 1.4-1.8MPa, Steam explosion treatment 0.5- to be passed through rapidly steam to steam explosion pressure inside the tank
After 2.5min, room temperature is concentrated into relative density 1.10-1.20, is spray-dried concentrated solution, obtains powder body；
S2, in powder body add 3-8 times of water, by mass percentage be 2-3% ratio add neutral protease, by quality percentage
Than flavor protease being added for 1-2%, digest 1-2h, filter, obtain enzymolysis solution at 30-50 DEG C；
S3, by naturally to thaw again after the fermentation liquid quick freeze of gained, make age of starch precipitation in extract；Collect supernatant,
Remainder is centrifuged, and collects centrifugal liquid and is incorporated in supernatant；
S4, by supernatant with the flow velocity of 3-7BV/h by macroporous resin column, after dynamic adsorption saturation, deionized water is with 5-
The above-mentioned macroporous resin column of flow velocity drip washing of 8BV/h is colourless to effluent, then with the organic solvent that volume fraction is 50%-70% with
The flow velocity of 8-12BV/h carries out eluting, collects eluent, and lyophilization obtains Herba Dendrobii lyophilized powder.
3. a kind of Herba Dendrobii as claimed in claim 1 promotes skin regeneration combination anti-senile preparation, it is characterised in that described
Fructus Canarii albi berry extract is as obtained by prepared by following steps：
The Fructus Canarii albi berry extract cleaned is placed in into 90% methanol or ethanol solution that flash extracter adds 6 times of volumes, 1-3 is processed
Minute, after solid-liquid separation, reclaim methanol or ethanol solution, concentration so that extract is in paste, then with water-saturated n-butanol pair
Extract is extracted 3 times, after recycling design, by drying extract concentrated in vacuo, obtains Fructus Canarii albi berry extract.
4. a kind of Herba Dendrobii as claimed in claim 1 promotes skin regeneration combination anti-senile preparation, it is characterised in that described
Catalpa leaf extract is as obtained by prepared by following steps：
S1, by clean Folium Ginkgo be placed in flash extracter process after, by mass percentage be 3-5% add complex enzyme hydrolysis liquid,
20-30 DEG C, 1-2 hours are digested, once, control pH value is between 3.5-6.2 for stirring, oxygenation per half an hour；
Enzymolysis solution is warming up to 85-100 DEG C after terminating by S2, enzymolysis, enzyme denaturing 30-40 minutes, is filtered, get Sheng Mali Ji extracting solution.
5. a kind of Herba Dendrobii as claimed in claim 1 promotes skin regeneration combination anti-senile preparation, it is characterised in that described
Armeniaca mume Sieb. yeast powder is as obtained by prepared by following steps：
Take clean drain after Armeniaca mume Sieb. be placed in steam-explosion jar, be first passed through nitrogen to steam explosion pressure inside the tank be 0.7-1.5MPa, explosion
Process 7-23min；Then it is 1.5-2.1MPa, Steam explosion treatment 0.8-2.8min to be passed through rapidly steam to steam explosion pressure inside the tank
Afterwards, according to the ratio microbe inoculation leaven of inoculum concentration 1.5-1.7%, keeping temperature 25-35 DEG C, after fermentation 48-54 hours,
High temperature sterilize, obtains Armeniaca mume Sieb. yeast powder.
6. a kind of Herba Dendrobii as claimed in claim 5 promotes skin regeneration combination anti-senile preparation, it is characterised in that described
Microbe leaven is by lactobacilluss, Bacillus licheniformis, Corynebacterium glutamicum in mass ratio 3:2:1 mixing gained.
7. a kind of Herba Dendrobii as claimed in claim 4 promotes skin regeneration combination anti-senile preparation, it is characterised in that described
Component of the complex enzyme hydrolysis liquid comprising following weight portion：Neutral protease 3-5 parts, flavor protease 2-5 parts.
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| CN109806190A (en) * | 2019-04-04 | 2019-05-28 | 烟台新时代健康产业日化有限公司 | A kind of fermenting extraction process of pollen pini |
| CN109806204A (en) * | 2019-04-04 | 2019-05-28 | 烟台新时代健康产业日化有限公司 | A kind of fermenting extraction process of green plum-blossom |
| CN115144491A (en) * | 2022-06-21 | 2022-10-04 | 安徽农业大学 | Method for purifying and detecting 6-hydroxydendrobine in fresh dendrobium huoshanense stems |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109806190A (en) * | 2019-04-04 | 2019-05-28 | 烟台新时代健康产业日化有限公司 | A kind of fermenting extraction process of pollen pini |
| CN109806204A (en) * | 2019-04-04 | 2019-05-28 | 烟台新时代健康产业日化有限公司 | A kind of fermenting extraction process of green plum-blossom |
| CN115144491A (en) * | 2022-06-21 | 2022-10-04 | 安徽农业大学 | Method for purifying and detecting 6-hydroxydendrobine in fresh dendrobium huoshanense stems |
| US11885777B2 (en) | 2022-06-21 | 2024-01-30 | Anhui Agricultural University | Method for purifying and detecting 6-hydroxynobilonine in fresh stems of dendrobium huoshanense |
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