CN106509902A - Lycopene-loaded nano-emulsion and preparation method thereof - Google Patents

Abstract

The invention belongs to the technical field of preparation technology of functional nutritious food, and in particular relates to a nanoemulsion for alleviating the oxidative decomposition of lycopene and a preparation method thereof. The present invention is composed of an oil phase and a water phase, the mass ratio of the oil and water phases is 1:11-1:7, the macromolecular emulsifier is dissolved in water as the water phase, lycopene is dissolved in the oily substance as the oil phase, and the oil and water phases are mixed The loaded lycopene nanoemulsion was prepared after emulsification and dispersion. The nanoemulsion loaded lycopene proposed by the present invention has the characteristics of high safety performance and high loading rate, mild action conditions, and lycopene is a sensitive active substance, which can improve the loading efficiency of lycopene and simultaneously load lycopene The characteristics of the absorption, metabolism, distribution and excretion of the nanoemulsion particles in the human body can improve its bioavailability, and have a great market prospect.

Description

A kind of loaded lycopene nanoemulsion and preparation method thereof

technical field

The invention belongs to the technical field of preparation technology of functional nutritious food, and in particular relates to a nanoemulsion that relieves and protects lycopene from oxidative decomposition and a preparation method thereof.

Background technique

Lycopene has the health care function of improving human body function and health, but it is difficult to add directly to food or be absorbed by the human body due to its insolubility in water, low bioavailability, and sensitivity to the environment, processing flow, and gastrointestinal tract conditions. Absorbs effectively.

At present, the technology that can effectively protect lycopene from being oxidized and decomposed is mainly microencapsulation technology, that is, the target substance is embedded by selecting a suitable film-forming material to play a protective role. For example, using sucrose plus gelatin or some other composite wall materials to prepare lycopene microcapsules by spray-drying method, there are also good results achieved by constructing lycopene water inclusions, liposomes, microemulsion systems and nanodispersion systems , but these technologies mainly focus on the micron level.

Nano-carrier technology is an in-depth development of microcapsule technology. Compared with ordinary emulsion (1-100 μm), nano-emulsion technology has many advantages that can be used in food and beverage product systems. First of all, the small size effect and surface effect unique to nanoemulsions, in addition to the protection of nutrients, nanocarriers often show higher stability and more excellent in vivo absorption, controlled release and targeting functions , thereby improving the bioavailability of lycopene; secondly, the nano-scale particle size can better stabilize the particles and prevent emulsion separation caused by aggregation or gravity. Moreover, due to the small particle size, the light scattering wave of the particles contained in the nanoemulsion is weak, so the emulsion is transparent or only slightly turbid, which is beneficial to its addition in various food and beverage systems.

Even if the technology of some products on the market reaches the nanoscale level, the synthetic or semi-synthetic small molecule emulsifiers used in the products are potentially toxic and added in large amounts, which inevitably brings hidden dangers to food safety.

Contents of the invention

The purpose of the present invention is to overcome the deficiencies and defects of the existing microcapsule technology and other nano-carrier technologies, provide a nanoemulsion that can effectively slow down the oxidative decomposition of lycopene, improve the retention rate of lycopene, and the technical conditions are mild. The technological process is simple and the level of mechanization is high, which provides the basis for actual production and application.

The purpose of the present invention is achieved through the following technical solutions:

A lycopene-loaded nanoemulsion is composed of an oil phase and a water phase, and the mass ratio of the oil-water phase is 1:11-1:7.

Further, the oil phase solution is one or more of medium-chain triglycerides (MCT), long-chain triglycerides (LCT), olive oil, peppermint oil, and lycopene.

Further, the aqueous phase solution is composed of a macromolecular emulsifier and deionized water.

Further, the macromolecular emulsifier is one or more of modified starch, sodium caseinate, and β-lactoglobulin.

Further, the mass fraction of the macromolecular emulsifier in the water phase is 20%-30%.

Another object of the present invention is to provide a kind of preparation method of loaded lycopene nanoemulsion, concrete steps are as follows:

S1: mix macromolecular emulsifier and deionized water evenly, as aqueous phase solution;

S2: mix the lycopene and the oil phase substance evenly, and ultrasonically dissolve it at normal temperature as the oil phase solution;

S3: Mix the oil phase and the water phase according to the mass ratio, and stir and emulsify at 30~70°C;

S4: After the S3 mixed solution is dispersed at high speed, it is homogenized under a high-pressure homogenizer to obtain a lycopene-loaded nanoemulsion.

Preferably, the macromolecular emulsifier in step S1 is stirred and dissolved at 30-70°C, and then stirred at room temperature to fully hydrate it.

Preferably, the mass fraction of lycopene in step S2 is 0.1-0.5%.

Preferably, the oil phase in step S2 is completely dissolved and then filtered.

Preferably, the oil-water mixture in step S3 is emulsified at 30°C.

More preferably, in step S3, the oil-water ratio is 1:11 and emulsified at 50-70°C.

Preferably, the mixed liquid described in step S4 is homogeneously prepared at 90-120 Mpa after high-speed dispersion to prepare the loaded lycopene nanoemulsion.

The present invention has the following beneficial effects:

The nanoemulsion loading lycopene proposed by the present invention has the characteristics of high safety performance and high loading rate. Compared with other technologies, the nanoemulsion technology has mild action conditions, and lycopene is a sensitive active substance, which can improve the lycopene. At the same time, the characteristics of absorption, metabolism, distribution and excretion of lycopene-loaded nanoemulsion particles in the human body can improve its bioavailability, which has a great market prospect.

Description of drawings

Figure 1 is the effect of different oil-water ratios on the retention rate of lycopene.

Figure 2 is the effect of different emulsification temperatures on the retention rate of lycopene.

Figure 3 is the effect of different treatment methods on the retention rate of lycopene.

detailed description

The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments, but the embodiments do not limit the present invention in any form. Unless otherwise specified, the methods and equipment used in the present invention are conventional reagents, methods and equipment in the technical field. Unless otherwise specified, the materials used in the following examples are commercially available.

Example 1 Investigating the Effects of Different Oil-Water Ratio on the Retention Rate of Lycopene

Group 1: a loaded lycopene nanoemulsion, consisting of an oil phase and a water phase, the mass ratio of the oil to water phase is 1:11, the oil phase is composed of MCT and lycopene, and the water phase is composed of modified starch and detoxified starch Composition of ionized water.

Group 2: a loaded lycopene nanoemulsion, consisting of an oil phase and a water phase, the mass ratio of the oil to water phase is 1:9, the oil phase is composed of MCT and lycopene, and the water phase is composed of modified starch and detoxified starch Composition of ionized water.

Group 3: a loaded lycopene nanoemulsion, consisting of an oil phase and a water phase, the mass ratio of the oil to water phase is 1:7, the oil phase is composed of MCT and lycopene, and the water phase is composed of modified starch and detoxified starch Composition of ionized water.

A preparation method for loading lycopene nanoemulsion, the specific steps are as follows:

S1. Configure a modified starch solution with a mass concentration of 20-30%, stir and dissolve it at 30-70 °C, and then put it at room temperature and stir to make it fully hydrated as the water phase;

S2. Configure lycopene oil with a mass concentration of 0.1-0.5% (w/w), dissolve it ultrasonically at room temperature, and filter it after it is completely dissolved, as the oil phase;

S3. Mix the oil phase and the water phase according to the mass ratio, and stir and emulsify at 30~70°C;

S4. After the mixed liquid is dispersed at high speed, it is homogenized three times at 110 Mpa to obtain the final loaded lycopene nanoemulsion.

Prepared according to the above method, after storage for one month, the retention rate of three groups of lycopene was measured by ultraviolet light absorption method, and the test results are shown in Fig. 1 . Experimental data showed that when the mass ratio of oil to water was 1:11, the retention rate of lycopene was the highest.

Example 2 Investigating the effects of different emulsification temperatures on the retention rate of lycopene

A loaded lycopene nanoemulsion, composed of an oil phase and a water liquid, the mass ratio of the oil to water phase is 1:11, the oil phase is composed of MCT and lycopene, and the water phase is composed of modified starch and deionized water .

Group 4: a preparation method of loaded lycopene nanoemulsion, the specific steps are as follows:

S1. Configure a modified starch solution with a mass concentration of 20-30%, stir and dissolve it at 30-70 °C, and then put it at room temperature and stir to make it fully hydrated as the water phase;

S2. Configure lycopene oil with a mass concentration of 0.1-0.5%, ultrasonically dissolve it at room temperature, and filter it after it is completely dissolved, as the oil phase;

S3. Mix the oil phase and the water phase at a mass ratio of 1:11, and stir and emulsify at 30°C;

S4. After the mixed liquid is dispersed at high speed, it is homogenized three times at 110 Mpa to obtain the final loaded lycopene nanoemulsion.

Group 5: The preparation method is the same as that of Group 4, except that the emulsification temperature in step S3 is 50°C.

Group 6: The preparation method is the same as that of Group 4, except that the emulsification temperature in step S3 is 70°C.

Prepared according to the above method, after one month of storage, the retention rate of the three groups of lycopene was measured by the ultraviolet absorbance method, and the results are shown in Figure 2. The test data shows that with the increase of temperature, the retention rate of lycopene in the emulsion has a process of improvement, and the change is not obvious after 50°C.

Example 3 Investigate the effects of different treatment methods on the retention rate of lycopene

Group 7: A nanoemulsion loaded with lycopene, consisting of an oil phase solution and a water phase solution, the mass ratio of the oil and water phases is 1:11, the oil phase is composed of MCT and lycopene, and the water phase is composed of modified starch and deionized water.

A preparation method for loading lycopene nanoemulsion, the specific steps are as follows:

S1. Configure a modified starch solution with a mass concentration of 20-30%, stir and dissolve it at 30-70 °C, and then put it at room temperature and stir to make it fully hydrated as the water phase;

S2. Configure lycopene oil with a mass concentration of 0.1-0.5%, ultrasonically dissolve it at room temperature, and filter it after it is completely dissolved, as the oil phase;

S3. Mix the oil phase and the water phase according to the mass ratio, and stir and emulsify at 50°C;

S4. After the mixed solution is dispersed at high speed, homogenize it for 3 times under 110Mpa to obtain the final loaded lycopene nanoemulsion.

The loaded lycopene nanoemulsion was prepared according to the above method, placed at room temperature, and the lycopene retention rate was measured by the ultraviolet light absorbance method every other week.

Control group 1: prepare lycopene oil with a mass concentration of 0.1%, ultrasonically dissolve it at room temperature, filter it and place it at room temperature, and measure its absorbance every one week. The oil phase substance used is MCT.

Result is as shown in accompanying drawing 3, and test data shows, group 7 still has 75.6% retention rate through nanoemulsion loaded lycopene after one month of storage, and the lycopene content directly soluble in oil phase of comparison group 1 is in storage It is already 0 in the 2nd week. The experimental results show that the lycopene supported by the nanoemulsion has good stability, can effectively alleviate the oxidative decomposition of lycopene, and has a longer retention time and a higher retention rate than the lycopene directly soluble in the oil phase.

The above-mentioned embodiments only express several implementation modes of the present invention, and the description thereof is relatively specific and detailed, but should not be construed as limiting the patent scope of the present invention. It should be pointed out that those skilled in the art can make several modifications and improvements without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent for the present invention should be based on the appended claims.

Claims (10)

1. a kind of to load lycopene nanoemulsions, it is characterised in that by oil phase and water phase composition, profit phase mass ratio is 1: 11-1:7。

2. lycopene nanoemulsions are loaded according to claim 1, it is characterised in that the oil-phase solution is medium chain triglyceride Three sour fat（MCT）, long-chain triglycerides (LCT), olive oil, one or more and lycopene in Oleum menthae.

3. lycopene nanoemulsions are loaded according to claim 1, it is characterised in that the aqueous phase solution is by macromole breast Agent and deionized water composition.

4. lycopene nanoemulsions are loaded according to claim 3, it is characterised in that the macromole emulsifying agent is degeneration One or more in starch, sodium caseinate, beta lactoglobulin.

5. lycopene nanoemulsions are loaded according to claim 4, it is characterised in that macromole emulsifying agent quality in water phase Fraction is 20% ~ 30 %.

6. it is a kind of load lycopene nanoemulsions preparation method, it is characterised in that step is as follows：

S1:By macromole emulsifying agent and deionized water mix homogeneously, as water phase；

S2:By lycopene and oil phase substance mix homogeneously, ultrasonic dissolution under room temperature, used as oil phase；

S3:Oil phase and water are mutually mixed in mass ratio, and the stirring and emulsifying at 30 ~ 70 DEG C；

S4:After S3 mixed liquors are disperseed at a high speed, under high pressure homogenizer, homogenizing obtains loading lycopene nanoemulsions.

7. the preparation method of lycopene nanoemulsions is loaded according to claim 6, it is characterised in that macromole emulsifying agent Stirring and dissolving is wanted, is put to being stirred at room temperature afterwards so as to abundant aquation.

8. the preparation method of lycopene nanoemulsions is loaded according to claim 6, it is characterised in that the lycopene Mass fraction be 0.1-0.5%.

9. the preparation method of lycopene nanoemulsions is loaded according to claim 6, it is characterised in that oil-water ratio 1:11 Mixed liquor is emulsified at 50 ~ 70 DEG C.

10. the preparation method of lycopene nanoemulsions is loaded according to any one of claim 6-9, it is characterised in that mixed Close liquid and homogenize load lycopene nanoemulsions Jing after high speed is disperseed under 90 ~ 120 Mpa.