CN1064082C - Bioenzyme used for dismounting pictures and the method therefor - Google Patents

Abstract

The unmounting solution made of biological enzyme and the method for unmounting and decontamination thereof. The mounting fluid includes two kinds of enzyme solutions used in conjunction with each other; the lysozyme aqueous solution prepared by the ratio of 120-220u activity in every 100ml solution by powder lysozyme and water, and the powder α-amylase and water by Each 100ml solution contains 30-90u activity ratio to prepare α-amylase aqueous solution. The unmounting method is divided into dry method and wet method. The dry method is brushing-constant temperature method, and the wet method is soaking method. The decontamination of "archive bricks" damaged by microbial secretions, dust, adhesives and other dirt has the advantages of fast, convenient, safe and economical.

Description

Bioenzyme unmounting liquid and its unmounting method

The invention relates to a mounting solution made of biozyme and a method for decontaminating the mounting.

In the archives of ancient books, most of the carrier materials are handmade paper, and the main component of handmade paper is cellulose. However, with the passage of time, archival classics are inevitably damaged in many ways in the process of preservation and use. In addition to light, harmful gases, and temperature and humidity changes, it is also damaged by serious dirt, which includes: microbial secretions, oil spots, stains, dust, and stale viscous agents. Among them, microbial secretions, dust, adhesives, etc. are more serious damage to archives. Under humid conditions, the adhesives and secretions of archives and classics are easy to react with dust and harmful gases. Under the pressure of itself and the outside world, the pages of the paper will be tightly bonded together, making the archives book They are hard and brittle, like bricks, so they are called "archive bricks" or "documentary bricks". Traditional exposing methods include dry method, wet method and high-humidity fumigation method. The dry method is to insert a thin bamboo piece into the gap between the pages of historical documents that are not seriously bonded, let the bamboo piece stick to the bottom page, move left and right to separate the adhesion between the pages of the document, and separate them . This method is suitable for files that are not seriously bonded. The wet method is to use hot water or boiling water to wash the dirt inside and outside the "archive brick". This method is suitable for mud spots and lighter adhesive soiling in archival documents. The high-temperature fumigation method is to put the hard and strong "archive bricks" in the pot, and use high-temperature steam to fumigate the dirt in the "archive bricks". However, for "archive bricks" with serious microbial contamination, the dirt has strong chemical stability, is insoluble or insoluble in water, and is not sensitive to high temperatures. The paper of precious archival documents becomes very brittle and difficult to preserve. To sum up, the traditional unmounting method has the following disadvantages: due to manual operation, it is very troublesome, and the unmounting time is slow, which is far from meeting the needs of piles of calligraphy and paintings waiting to be repaired; or due to manual operation, only personal experience can be used. , It is inconvenient to master, and sometimes improper operation may damage precious cultural relics. Therefore it is necessary to find a fast, convenient, safe and economical method to replace this slow, cumbersome, dangerous and uneconomical traditional decontamination method.

The purpose of the present invention is to realize a kind of demounting liquid that biological enzyme element is made and the method for decontaminating and decontaminating thereof, be to make demounting liquid with biological enzyme element, use this demounting liquid to remove mounting, decontamination, have Fast, convenient, safe and economical advantages.

The present invention is achieved in the following manner: the enzyme-containing aqueous solution made of biological enzymes is used as the mounting solution for "archive bricks" and "document bricks", which can be used for "archive bricks" or "document bricks" damaged by dirt. "Carry out exposing and decontaminating.

Due to the different types of "archive bricks" or "document bricks" damaged by dirt, such as ancient calligraphy and paintings, imperial lists, or files, memorials, and ancient books; different storage locations; different paper properties , so in fact, the damage of dirt to ancient borrowings, archives, and documents is very complicated. However, from the perspective of the dirt that destroys "archive bricks" or "document bricks", the dirt can be divided into three types: (1) pure starch binder dirt, (2) secretions of pure microorganisms, ( 3) The joint action of starch binder, dust and microorganisms. The use of enzyme liquid to decontaminate is the mechanism of using enzymes to react with dirt. For example, the use of enzymes to hydrolyze starch paste dirt can treat pure starch binder dirt, and different properties Enzymes have specificity in response to different dirt, so the bioenzyme mounting solution of the present invention is prepared according to the different situations and degrees of destruction of "archive bricks" or "document bricks" damaged by dirt. Three kinds of mounting solutions containing different enzymes were prepared.

Bioenzyme Mounting Solution No. 1 is suitable for "archive bricks" or "documentary bricks" damaged by stale starch binders, dust and other dirt. The bioenzyme mounting solution No. 1 includes the following two enzyme solutions used in conjunction with each other:

(1) The aqueous solution of lysozyme prepared by mixing powdered lysozyme and water according to the ratio of 120-220u activity per 100ml solution, the pH of the solution is 5.8-6.8, and the storage temperature is 0°C-10°C ℃;

(2) Prepare the a-amylase aqueous solution by mixing powder a-amylase and water according to the ratio of 30-90u activity per 100ml solution, the pH of the solution is 7-7.8, and the storage temperature is 0°C- 10°C.

Bioenzyme Mounting Solution No. 2 is suitable for "archive bricks" or "documentary bricks" damaged by microorganisms, pest secretions, dust and other dirt. Biozyme Mounting Solution No. 2 includes the following two kinds of mutual Compound enzyme solution used:

(1) The composite aqueous solution of lysozyme and glucoamylase is a lysozyme aqueous solution prepared by powder lysozyme and water according to the ratio of 150-250u activity per 100ml solution, and the pH of the solution is 5.8-6.8 , and then the solution of glucoamylase and water according to the ratio of 30-90u activity in each 100ml solution to prepare the glucoamylase aqueous solution, the pH of the solution is 6.0-6.7, and finally the above two enzyme solutions are mixed by 1 : 1 is prepared by mixing equal volumes, and the storage temperature of the complex enzyme solution is 0°C-10°C;

(2) The composite aqueous solution of trypsin and bacillus subtilis neutral protease is the trypsin aqueous solution prepared by powder trypsin and water according to the ratio of 100-200u activity in every 100ml solution, and the pH of this solution is 7.0- 7.7, then powder bacillus subtilis neutral protease and water are prepared by the ratio of 120-220u activity in every 100ml solution to prepare bacillus subtilis neutral protease aqueous solution, the pH of this solution is 7.0-7.8, finally It is prepared by mixing the above two enzyme solutions in an equal volume ratio of 1:1, and the storage temperature of the composite enzyme solution is 0°C-10°C.

Bioenzyme Mounting Solution No. 3 is suitable for "archive bricks" or "documentary bricks" damaged by starch binders, secretions of microorganisms, pests, and dust. The Bioenzyme Mounting Solution No. 3 includes The following two complex enzyme solutions are used in conjunction with each other:

(1) The composite aqueous solution of lysozyme and glucoamylase is a lysozyme aqueous solution prepared by mixing powdered lysozyme and water according to the ratio of 120-220u activity per 100ml solution, and the pH of the solution is 6.0-6.7 , and then the solution of glucoamylase and water according to the ratio of 30-90u activity in each 100ml solution to prepare the glucoamylase aqueous solution. The pH of the solution is 5.8-6.8, and finally the above two enzyme solutions : 1 is prepared by mixing equal volumes, and the storage temperature of the complex enzyme solution is 0°C-10°C;

(2) α-amylase, trypsin, the composite aqueous solution of bacillus subtilis neutral protease, is that powder α-amylase and water contain the α-amylase aqueous solution of 30-90u activity in every 100ml solution, the pH of this solution If the pH of the solution is 7.0-7.5, the trypsin aqueous solution prepared by mixing powder trypsin and water according to the ratio of 120-220u activity per 100ml solution, the pH of the solution is 7.0-7.8, and then the powder Bacillus subtilis neutral protease and water contain the bacillus subtilis neutral protease aqueous solution prepared by the proportioning ratio of 120-220u activity in 100ml solution, the pH of this solution is 7.0-7.6, finally the above three kinds of enzyme solutions are pressed by 1 : 1:1 mixed with equal volumes, the storage temperature of the complex enzyme solution is 0°C-10°C.

The above-mentioned bioenzyme unmounting solution is generally stored in a yellow glass bottle at a temperature of 0°C-10°C, and the validity period is within 10 months.

The mounting methods of Biozyme Mounting Solution No. 1, No. 2, and No. 3 can be divided into dry method and wet method.

Dry method: each label of bioenzyme unmounting solution is carried out in the following way, according to the order of the serial numbers (1) and (2) of the enzyme solution, use the serial number (1) enzyme Solution, serial number (2) Enzyme solution carries out brushing-constant temperature operation to "archives and document bricks" successively, the brushing-constant temperature operation process is: first use the enzyme solution to brush on "archives and document bricks" , then wrap it with pure plant fiber cloth, then put it into a 35°C-40°C constant temperature box for 10-30 minutes, and the number of brushing-constant temperature operations carried out by the enzyme solution of the serial number (1) is 1-2 times, the number of operations of the brushing-constant temperature carried out by the enzyme solution of the serial number (2) is 1-2 times. When the amount is 30-40%, it can be unmounted, and each page of paper after unmounted is sprayed with an aqueous solution containing bromogeramine antifungal agent. The aqueous solution is prepared from bromogeramine antifungal agent and water, and the aqueous solution contains 0.1% (percentage by weight) of the bromogeramine antifungal agent. The pure plant fiber cloth used in the brushing-constant temperature operation is the cloth made of plant fibers such as cotton, wool and hemp. This dry method is suitable for unmounting paper with poor strength of "archive bricks" and "document bricks".

Wet method: each type of bioenzyme demounting solution is demounted in the following way, according to the sequence of the serial numbers (1) and (2) of the enzyme solution, the "archives and literature bricks" are sorted Soak in the enzyme solution of serial number (1) and enzyme solution of serial number (2) each time, the soaking temperature is 35°C-40°C, soak for 15-25 minutes each time, rinse with water after each soaking Clean, and finally after the tide is removed, when the moisture content of the "archive and document bricks" is 30-40%, it can be unmounted. This wet method is suitable for unmounting of paper with better strength for "archives and literature bricks".

Further illustrate with embodiment below again.

Example 1:

Prepare Bioenzyme Mounting Solution No. 1, which is suitable for "archives and literature bricks" damaged by starch binders, dust and other dirt. Prepare powdered lysozyme and water according to the ratio of 200u activity per 100ml solution to make lysozyme aqueous solution, pH is 6.3; prepare powder a-amylase and water according to the ratio of 50u activity per 100ml solution into a-amylase aqueous solution, pH 7.3.

Use the dry method to unmount the "archive and document bricks" with poor paper strength. Paint the lysozyme aqueous solution on the "archives and literature bricks", wrap the "archives and literature bricks" soaked in the enzyme solution with pure plant fiber cloth, and then put them in a constant temperature box at 37°C. After 20 minutes Take it out, remove the pure plant fiber cloth used for wrapping, and then brush it again with lysozyme aqueous solution, wrap it with pure plant fiber cloth, put it in the incubator at the above temperature again, take it out after 10 minutes, and remove it for wrapping Pure plant fiber cloth, then brush it again with a-amylase aqueous solution, wrap it with a clean pure plant fiber cloth, put it in a 37°C constant humidity box, take it out after 20 minutes, and remove the pure plant fiber for wrapping cloth, then brush it again with a-amylase aqueous solution, wrap it with pure plant fiber cloth, put it in the incubator at 37°C again, take it out after 20 minutes, remove the pure plant fiber cloth for wrapping, and Withdraw the tide, and when the moisture content of the "archives and literature bricks" is 30-40%, it can be unmounted. As long as a little pulling force is applied, the pages of the "archives and literature bricks" can be completely sealed. separate. In order to prevent mildew on the paper of "archives and literature bricks", an aqueous solution containing bromogeramine antifungal agent can be sprayed on the uncovered paper pages. % (percentage by weight) of the bromogermic antifungal agent.

Use the wet method to unmount the "archive and document bricks" with good paper strength. Immerse the "archives and literature bricks" in the lysozyme aqueous solution at a temperature of 37°C, take them out after 20 minutes, rinse them with clean water, and then immerse the rinsed "archives and literature bricks" in the a-amylase aqueous solution, soak The temperature is 37°C, take it out after 20 minutes, rinse with clean water, and remove the moisture. When the moisture content of the "archive and document brick" is 30-40%, it can be unmounted.

Example 2:

Prepare bioenzyme unmounting solution No. 2, which is suitable for "archives and literature bricks" damaged by microorganisms, pest secretions, dust and other dirt. Prepare powdered lysozyme and water according to the ratio of 200u activity per 100ml solution to make lysozyme aqueous solution, pH is 6.2; then prepare liquid glucoamylase and water according to the ratio of 50u activity per 100ml solution into an aqueous solution of glucoamylase with a pH of 6.2. And above-mentioned two kinds of enzyme solutions are mixed by 1: 1 equivolume and make compound enzyme solution (1); The trypsin aqueous solution prepared by the proportioning ratio that contains 160u activity in every 100ml solution with powder trypsin and water, pH is 7 .3, then powder Bacillus subtilis neutral protease and water according to the ratio of 160u activity in each 100ml solution to prepare the Bacillus subtilis neutral protease aqueous solution, the pH is 7.3, and the above two enzyme solutions are mixed at a ratio of 1:1, etc. Volume mixing to make complex enzyme solution (2).

Use the dry method to unmount the "archive and document bricks" with poor paper strength. Its operating process is basically the same as the dry process in Example 1, except that the temperature control of the incubator is different, which is 38° C., and other conditions are the same. That is, the number of brushing-constant temperature operations carried out by the compound enzyme solution of serial number (1) is 2 times, the time of constant temperature for the first time is 20 minutes, the time of constant temperature for the second time is 10 minutes, and then the time of serial number (2) The number of brushing-constant temperature operations performed by the compound enzyme solution is also 2 times, and the time for each constant temperature is 20 minutes. The temperature of the constant temperature box used in the above constant temperature process is 38°C. Finally, when the moisture content of "archives and literature bricks" is 30-40%, unmounting is carried out, and each page of paper after unmounting is sprayed with an aqueous solution containing bromogeramine antifungal agent, which contains 0.1% (percentage by weight) The new geramine antifungal agent.

Use the wet method to unmount the "archive and document bricks" with good paper strength. Its operating process is basically the same as the wet operating process in Example 1, except that the soaking temperature control is different, which is 38° C., and other conditions are all the same. That is to say, the "archives and literature bricks" are immersed in the compound enzyme solution of serial number (1) and the compound enzyme solution of serial number (2) one at a time. Rinse with clear water after the immersion process is completed, and finally remove the moisture, and when the moisture content of the "archive and document bricks" is 30-40%, it can be unmounted.

Example 3

Prepare Bioenzyme Mounting Solution No. 3, which is suitable for "archives and literature bricks" damaged by starch binders, secretions of microorganisms, pests, and dust. Prepare powdered lysozyme and water according to the ratio of 200u activity per 100ml solution to prepare lysozyme aqueous solution, pH is 6.3; then prepare liquid glucoamylase and water according to the ratio of 50u activity per 100ml solution into an aqueous solution of glucoamylase with a pH of 6.3. And above-mentioned two kinds of enzyme solutions are mixed by 1: 1 equivolume and make compound enzyme solution (1); Powder a-amylase and water contain the proportioning preparation a-amylase aqueous solution of 50u activity in every 100ml solution, The pH is 7.4, the powder trypsin and water are formulated into a trypsin aqueous solution according to the ratio of 160u activity per 100ml solution, and the pH is 7.4, then the powder Bacillus subtilisin neutral protease and water are mixed with 100ml solution containing The ratio of 160u activity is prepared into an aqueous solution of Bacillus subtilis neutral protease, with a pH of 7.4, and finally the above three enzyme solutions are mixed in equal volumes of 1:1:1 to prepare a complex enzyme solution (2).

Use the dry method to unmount the "archive and document bricks" with poor paper strength. Its operating process is the same as the dry process in Example 2, the number of brushing-constant temperature operations, the constant temperature time, and the temperature of the incubator are all the same.

Use the wet method to unmount the "archive and document bricks" with good paper strength. Its operating process is the same as the wet operating process in Example 2, and the dipping times, soaking time, and soaking temperature are all the same.

Using bio-enzyme method to hydrolyze the dirt of "archives and literature bricks", the effect of exposing and mounting is extremely obvious. The method of unmounting with biological enzymes not only avoids the damage to the archive paper caused by traditional high-temperature fumigation, but also has no side effects on historical documents after treatment. Because the enzyme in the mounting solution is non-toxic, tasteless and non-corrosive. The screening and purification of enzymes are completely natural, and the pH value of the enzyme solution is weakly alkaline. Experiments have shown that residual weak alkaline components in archival paper are beneficial and harmless to the life of archival paper, because it can be continuously neutralized. And the acidic components that regenerate in the archives after dropping. It is beneficial to the security of files. In addition, the mounting liquid of the present invention has almost no influence on the writing materials of carbon components.

The biological enzyme method is used to treat historical archives or historical documents bonded into bricks. Due to the analysis of the dirt components and properties in the "archives and document bricks", it is very easy to remove the sticky dirt by selecting a suitable enzyme solution. Thorough, with complete separation of archival paper pages from page to page.

It is a scientific and economical method to repair and salvage historical archives and documents using the biological enzyme method. It can save labor and time and achieve the best effect.

my country is an ancient civilization country, and there are many historical documents and files to be repaired and rescued. Therefore, the mounting fluid and mounting method of the present invention have a wide range of applications and have extensive social applicability.

Claims (3)

1, a kind of biological enzyme element unmounting liquid, it is characterized in that comprising the enzyme solution that following two kinds cooperate with each other: (1) The aqueous solution of lysozyme prepared by mixing powdered lysozyme and water according to the ratio of 120-220u activity per 100ml solution, the pH of the solution is 5.8-6.8, and the storage temperature is 0°C-10°C ℃; (2) Prepare the a-amylase aqueous solution by mixing powder a-amylase and water according to the ratio of 30-90u activity per 100ml solution, the pH of the solution is 7-7.8, and the storage temperature is 0°C- 10°C. 2, the method for unmounting of the described bioenzyme unmounting solution of claim 1, it is characterized in that according to the sequence number (1), (2) of enzyme liquid wherein, use described serial number (1) enzyme solution, serial number (2) Enzyme solution carries out brushing-constant temperature operation to "archives, literature bricks" successively, and this brushing-constant temperature operation process is: first brush on "archives, literature bricks" with described enzyme solution, and then use Wrap it with pure plant fiber cloth, then put it into a 35°C-40°C incubator to keep the temperature constant for 10-30 minutes, and the number of times of brushing-constant temperature operation carried out by the enzyme solution of the serial number (1) is 1-2 times. The operation times of brushing-constant temperature carried out by the enzyme solution of the serial number (2) is 1-2 times. When the temperature is -40%, it can be unmounted, and each page after unmounted is sprayed with an aqueous solution containing bromogeril antifungal agent. 3. The method for unmounting of the bio-enzyme unmounting solution according to claim 1 is characterized in that according to the order of the serial numbers (1) and (2) of the enzyme solution, the 'archives and literature bricks' are soaked once in succession In the enzyme solution of the serial number (1) and the enzyme solution of the serial number (2), the soaking temperature is 35°C-40°C, soaking for 15-25 minutes each time, rinsing with clear water after each soaking, and finally After the tide is withdrawn, when the moisture content of the "archive and document bricks" is 30-40%, the mounting can be carried out.