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CN106359306A - Method for improving locust egg laying amount and for collecting locust eggs - Google Patents

Method for improving locust egg laying amount and for collecting locust eggs Download PDF

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Publication number
CN106359306A
CN106359306A CN201610721648.7A CN201610721648A CN106359306A CN 106359306 A CN106359306 A CN 106359306A CN 201610721648 A CN201610721648 A CN 201610721648A CN 106359306 A CN106359306 A CN 106359306A
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locust
eggs
quartz sand
egg
locusts
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陈祥盛
刘洋洋
张余杰
母银林
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Guizhou University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/30Rearing or breeding invertebrates

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  • Environmental Sciences (AREA)
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Abstract

本发明公开了一种提高蝗虫产卵量及蝗虫卵收集方法,包括以下步骤:(1)培育水稻苗;(2)营造蝗虫饲养环境;(3)将野外采集的蝗虫成虫放在饲养笼中进行饲养,将水稻苗放入饲养笼中供蝗虫啃食,定时更换水稻苗;(4)待蝗虫产卵布满石英砂后取出盒体,加水将蝗虫卵块淘出,将卵块放入冰箱保,保存温度为4℃。本发明采用石英砂作为产卵基质,极大地提高蝗虫的产卵量;另外,采用石英砂作为产卵基质,在取蝗虫卵时,只需往石英砂中加水后即可将蝗虫卵淘出,不破换蝗虫卵卵块的整体结构,操作简单、方便,有效地提高了蝗虫卵的采集效率。The invention discloses a method for increasing locust egg production and locust egg collection, comprising the following steps: (1) cultivating rice seedlings; (2) building a locust breeding environment; (3) placing locust adults collected in the field in a breeding cage Carry out breeding, put the rice seedlings into the breeding cage for the locusts to eat, and replace the rice seedlings regularly; (4) Take out the box after the locusts have laid eggs and are covered with quartz sand, add water to remove the locust eggs, and put the eggs in the refrigerator for preservation. , and the storage temperature is 4°C. The present invention uses quartz sand as the oviposition substrate, which greatly increases the oviposition rate of locusts; in addition, the quartz sand is used as the oviposition substrate, and when taking locust eggs, just add water to the quartz sand to pan out the locust eggs , does not break the overall structure of locust egg blocks, simple and convenient operation, and effectively improves the collection efficiency of locust eggs.

Description

一种提高蝗虫产卵量及蝗虫卵收集方法A method for increasing locust egg production and locust egg collection

技术领域technical field

本发明涉及一种提高蝗虫产卵量及蝗虫卵收集方法。The invention relates to a method for increasing locust egg production and collecting locust eggs.

背景技术Background technique

芫菁类昆虫隶属鞘翅目Coleoptera、异附节类Heteromera、拟步甲总科Tenebrioidea、芫菁科Meloidae,该昆虫以复变态生活史、幼虫寄生性、成虫群居性、化学防御、耐旱性等有趣的生物学特性而显得较为奇特。其中芫菁部分类群的幼虫属于半寄生性捕食者,寄生于蝗虫卵囊或蜂巢中,取食蝗虫或蜜蜂种科昆虫的幼虫。芫菁成虫能分泌的斑蝥素,其属于萜类物质,有明显的抗癌活性和杀虫活性。芫菁在药理学、兽医学和农业上都表现出重要性。Turtle-like insects belong to Coleoptera, Heteromera, Tenebrioidea, and Meloidae. The insects have multiple metamorphosis life history, larval parasitism, adult gregariousness, chemical defense, drought tolerance, etc. Interesting biological properties make it seem strange. Among them, the larvae of some taxa of Coriander are semi-parasitic predators, parasitic in locust oocysts or hives, and feed on larvae of locusts or bee species. Cantharidin, which can be secreted by adults of Melaleuca japonica, belongs to terpenoids and has obvious anticancer activity and insecticidal activity. Turmeric has shown importance in pharmacology, veterinary medicine and agriculture.

芫菁是我国传统中药材之一,因其体内含有斑蝥素(Cantharidin,C10H12O4)而具有重要的药用价值。斑蝥素有剧毒,外用能蚀死肌、敷疥癣、除恶疮,内服有攻毒、逐瘀散结、抗肿瘤的作用。随着临床上斑蝥类药物对癌症及其他疾病治疗效果的发现,引起了人们对芫菁及其所含斑蝥素的普遍关注和广泛研究,目前芫菁已经被列为我国紧缺的药用动物之一。Turmeric is one of the traditional Chinese medicinal materials in China, which has important medicinal value because it contains Cantharidin (C 10 H 12 O 4 ). Cantharidin is highly toxic, and external use can erode dead muscles, apply scabies, and remove malignant sores, and oral administration has the effects of attacking toxins, dispelling blood stasis and dispelling stagnation, and antitumor. With the clinical discovery of the therapeutic effects of cantharidin drugs on cancer and other diseases, it has aroused widespread concern and extensive research on the cantharidin and its contained cantharidin. one.

近年来,随着中国经济持续、稳定的发展和人均生活水平的提高,关于芫菁的饲养还处于探索阶段,并没有大规模人工饲养的相关报道。所以一般都会密切关注其整个生活史和行为学等方面的情况,然而,芫菁饲养最困难的阶段为幼虫的饲养,主要是因芫菁幼虫以蝗虫卵为食,而蝗虫卵的获取较为困难。In recent years, with the continuous and stable development of China's economy and the improvement of per capita living standards, the breeding of coriander leaves is still in the exploratory stage, and there are no reports about large-scale artificial breeding. Therefore, we generally pay close attention to its entire life history and behavioral conditions. However, the most difficult stage of raising celery is the larvae, mainly because larvae feed on locust eggs, and it is difficult to obtain locust eggs. .

目前,饲养芫菁幼虫的蝗虫卵的获取方法,主要采用野外采集的方式获得,野外采集费时费力,蝗虫卵的获取量少,况且在野外,蝗虫往往将卵产于土层5cm以下,而具有少部分产在水稻根部桩上,进行采集时很难;在田间采集到蝗虫卵,即使从水稻桩上获取到蝗虫卵也是很有限的。总之,田间获取蝗虫卵不仅耗时耗力,而且采集到的蝗虫卵有限且整体结构不完整。At present, the method of obtaining locust eggs for feeding the larvae of B. japonica mainly adopts the method of field collection, which is time-consuming and laborious, and the amount of locust eggs obtained is small. Moreover, in the wild, locusts often lay their eggs below the soil layer 5cm, which has the advantages of A small part is produced on rice root piles, which is difficult to collect; locust eggs are collected in the field, even if locust eggs are obtained from rice piles, it is very limited. In short, obtaining locust eggs in the field is not only time-consuming and labor-intensive, but also the collected locust eggs are limited and the overall structure is incomplete.

发明内容Contents of the invention

本发明要解决的技术问题是:提供一种提高蝗虫产卵量及蝗虫卵收集方法,提高蝗虫的产卵量,同时蝗虫卵收集简单方便,省时省力,为芫箐幼虫的高效饲养打下基础。The technical problem to be solved by the present invention is to provide a method for increasing the egg production of locusts and collecting locust eggs, increasing the egg production of locusts, and at the same time collecting the eggs of locusts is simple and convenient, saving time and effort, and laying the foundation for the efficient feeding of genus larvae .

本发明的目的是通过以下技术方案实现的:The purpose of the present invention is achieved through the following technical solutions:

一种提高蝗虫产卵量及蝗虫卵收集方法包括以下步骤:A method for improving locust egg production and locust ovum collection comprises the following steps:

(1)培育水稻苗:将水稻种子浸种至露白,将已露白的水稻种子撒在有吸水纸的育苗盘上,放入人工气候箱内,温度设置为30~35℃,湿度为70%,光周期为14L:10D,进行催芽,出芽后,放入装有田间土样的塑料盆中育种且人工气候箱设置温度29℃,湿度为70%,光周期为14L:10D,适量加水,待水稻长至20-25cm,得到水稻苗,备用;(1) Cultivate rice seedlings: Soak the rice seeds until they are white, sprinkle the white rice seeds on a seedling tray with absorbent paper, put them in an artificial climate box, set the temperature at 30~35°C, and the humidity at 70%. The photoperiod is 14L:10D, and germination is carried out. After germination, it is put into a plastic pot with field soil samples for breeding, and the temperature of the artificial climate box is set at 29°C, the humidity is 70%, the photoperiod is 14L:10D, and an appropriate amount of water is added. Paddy rice grows to 20-25cm, and rice seedling is obtained, and is set aside;

(2)营造蝗虫饲养环境:将蝗虫饲养笼放置于人工气候箱内,其温度设置为28℃,湿度为70%,光周期为14L:10D,并在蝗虫饲养笼内放置敞口状的盒体,盒体内装有供蝗虫产卵用的石英砂,且保持石英砂的湿度为30%;(2) Create a locust breeding environment: place the locust breeding cage in an artificial climate box, set the temperature at 28°C, the humidity at 70%, and the photoperiod of 14L:10D, and place an open box in the locust breeding cage body, the box body is equipped with quartz sand for locusts to lay eggs, and the humidity of keeping the quartz sand is 30%;

(3)饲养蝗虫:将野外采集的蝗虫成虫放在饲养笼中进行饲养,同时将步骤(1)得到的水稻苗放入饲养笼中供蝗虫啃食,定时更换水稻苗;(3) Raising locusts: Put the adult locusts collected in the field in a breeding cage for feeding, and at the same time put the rice seedlings obtained in step (1) into the raising cages for the locusts to eat, and replace the rice seedlings regularly;

(4)收集蝗虫卵:蝗虫将卵产在石英砂中,待卵块布满石英砂后取出盒体,向盒体中加水,将蝗虫卵块淘出,淘出后的卵块放入冰箱保存即可,保存温度为4℃。(4) Collect locust eggs: locusts lay eggs in quartz sand, take out the box after the eggs are covered with quartz sand, add water to the box, pan out the locust eggs, and put the eggs in the refrigerator for storage , and the storage temperature is 4°C.

步骤(1)中,塑料盆为长40cm、宽30cm、高为12cm。In step (1), the plastic basin has a length of 40 cm, a width of 30 cm, and a height of 12 cm.

步骤(2)中,饲养笼的尺寸为长宽均为45cm、高60cm。In step (2), the size of the breeding cage is 45 cm in length and width, and 60 cm in height.

步骤(2)中,盒体为长25cm、宽14cm、高为5cm的塑料盒。In step (2), the box body is a plastic box with a length of 25 cm, a width of 14 cm, and a height of 5 cm.

与现有技术相比,本发明的有益效果如下:Compared with the prior art, the beneficial effects of the present invention are as follows:

1、采用石英砂作为产卵基质,极大地提高蝗虫的产卵量;另外,采用石英砂作为产卵基质,在取蝗虫卵时,只需往石英砂中加水后即可将蝗虫卵淘出,不破换蝗虫卵卵块的整体结构,操作简单、方便,有效地提高了蝗虫卵的采集效率。1. Using quartz sand as the oviposition substrate greatly increases the oviposition of locusts; in addition, using quartz sand as the oviposition substrate, when taking locust eggs, you only need to add water to the quartz sand to pan out the locust eggs , does not break the overall structure of locust egg blocks, simple and convenient operation, and effectively improves the collection efficiency of locust eggs.

2、本发明所述方法便捷高效的解决了芫菁幼虫食物的来源,省时省力省钱,所采集到的蝗虫卵质量也提供了保障,为芫箐幼虫的高效饲养打下基础。2. The method of the present invention conveniently and efficiently solves the source of food for the larvae of Coriander japonica, saves time, labor and money, and the quality of the collected locust eggs is also guaranteed, laying a foundation for the efficient feeding of larvae of cilantro japonica.

具体实施方式detailed description

下面结合具体的实施例对发明进行进一步介绍:The invention is further described below in conjunction with specific embodiments:

实施例:Example:

一种提高蝗虫产卵量及蝗虫卵收集方法,包括以下步骤:A method for improving locust egg production and locust egg collection, comprising the following steps:

(1)培育水稻苗:将水稻种子浸种至露白,将已露白的水稻种子撒在有吸水纸的育苗盘上,放入人工气候箱内,温度设置为30~35℃,湿度为70%,光周期为14L:10D,进行催芽;出芽后,放入装有田间土样的塑料盆中育种且人工气候箱设置温度29℃,湿度为70%,光周期为14L:10D,适量加水,待水稻长至20-25cm,得到水稻苗,备用;所采用的塑料盆为长40cm、宽30cm、高为12cm。(1) Cultivate rice seedlings: Soak the rice seeds until they are white, sprinkle the white rice seeds on a seedling tray with absorbent paper, put them in an artificial climate box, set the temperature at 30~35°C, and the humidity at 70%. The photoperiod is 14L:10D for accelerating germination; after germination, put it into a plastic pot with field soil samples for breeding and set the temperature in the artificial climate box at 29°C, the humidity is 70%, and the photoperiod is 14L:10D. Paddy rice grows to 20-25cm, obtains rice seedling, for subsequent use; The plastic basin that adopts is long 40cm, wide 30cm, height is 12cm.

(2)营造蝗虫饲养环境:将蝗虫饲养笼放置于人工气候箱内,人工气候箱的温度为28℃,湿度为70%,光周期为14L:10D,并在蝗虫饲养笼内放置敞口状的盒体,盒体内装有供蝗虫产卵用的石英砂,且保持石英砂的湿度为30%,所采用的饲养笼长宽均为45cm、髙为60cm,所采用的盒体为长25cm、宽14cm、高为5cm的塑料盒;(2) Create a locust breeding environment: place locust breeding cages in an artificial climate box with a temperature of 28°C, a humidity of 70%, and a photoperiod of 14L:10D, and place an open-faced The box body is equipped with quartz sand for locusts to lay eggs, and the humidity of the quartz sand is kept at 30%. The length and width of the adopted cage are 45cm, and the height is 60cm. , a plastic box with a width of 14cm and a height of 5cm;

(3)饲养蝗虫:将野外采集的蝗虫放在饲养笼中进行饲养,同时将步骤(1)得到的水稻苗放入饲养笼中供蝗虫啃食,定时更换水稻苗;(3) Raising locusts: put the locusts collected in the field in breeding cages for raising, and at the same time put the rice seedlings obtained in step (1) into the raising cages for the locusts to eat, and replace the rice seedlings regularly;

(4)收集蝗虫卵:蝗虫将卵产在石英砂中,待卵块布满石英砂后取出盒体,向盒体中加水,将蝗虫卵块淘出,淘出后的卵块放入冰箱保存即可,保存温度为4℃。(4) Collect locust eggs: locusts lay eggs in quartz sand, take out the box after the eggs are covered with quartz sand, add water to the box, pan out the locust eggs, and put the eggs in the refrigerator for storage , and the storage temperature is 4°C.

在按照上述步骤,在蝗虫饲养时,设置六个处理组,即设置六个蝗虫饲养笼,在每个饲养笼中放入5龄的蝗虫25对,最后分别采集每个饲养笼中蝗虫在产卵基质石英砂上的蝗虫卵,并分别统计每个处理组的产卵量,得到试验组数据(见表1)。According to the above-mentioned steps, when locusts were raised, six treatment groups were set, that is, six locust raising cages were set, and 25 pairs of locusts of the 5th age were put into each raising cage, and finally the locusts in each raising cage were collected respectively when they were born. Grasshopper eggs on the egg matrix quartz sand, and the egg production of each treatment group were counted separately to obtain the data of the test group (see Table 1).

对照例:Comparative example:

在营造蝗虫饲养环境,将饲养笼中盒体内供蝗虫产卵的石英砂替换为野外采集的湿度为30%的田间土样,其余条件与实施例相同,同样设置六个处理组,即设置六个蝗虫饲养笼,在每个饲养笼中放入5龄的蝗虫25对,最后分别采集每个饲养笼中蝗虫在产卵基质田间土样上的蝗虫卵,并分别统计每个处理组的产卵量,得到对照组数据(见表1)In building the locust breeding environment, the quartz sand for the locusts to lay eggs in the box body of the raising cage is replaced by a field soil sample with a humidity of 30% collected in the field. 2 locust rearing cages, put 25 pairs of locusts of the 5th instar in each raising cage, collect the locust ovum of locusts in each raising cage respectively on the field soil sample of the oviposition substrate respectively at last, and count the production of each treatment group respectively The amount of eggs was obtained from the data of the control group (see Table 1)

结果表明:经过试验组和对照组对比可知,证明蝗虫喜欢在石英砂作为产卵基质中产卵,且产卵量比在田间土样中提高了39.4%。而且,蝗虫卵产于田间土样中,既不方便寻找,又不方便取卵;相反,蝗虫产卵于普通石英砂中,卵很方便取得,只需要在取卵时,在石英砂中加入一定量的水,就可以把蝗虫卵淘出。The results show that: after comparing the test group and the control group, it can be seen that locusts like to lay eggs in quartz sand as the oviposition substrate, and the amount of eggs laid is 39.4% higher than that in field soil samples. Moreover, locust eggs are laid in soil samples in the field, which is inconvenient to find and take eggs. On the contrary, locusts lay eggs in ordinary quartz sand, and eggs are easy to get. You only need to add A certain amount of water can wash out the locust eggs.

以上内容是结合具体的优选实施方式对本发明所作的进一步详细说明,不能认定本发明的具体实施只局限于这些说明。对于本发明所属技术领域的普通技术人员来说,在不脱离本发明构思的前提下,还可以做出若干简单推演或替换,都应当视为属于本发明的保护范围。The above content is a further detailed description of the present invention in conjunction with specific preferred embodiments, and it cannot be assumed that the specific implementation of the present invention is limited to these descriptions. For those of ordinary skill in the technical field of the present invention, without departing from the concept of the present invention, some simple deduction or replacement can be made, which should be regarded as belonging to the protection scope of the present invention.

Claims (4)

1. a kind of locust egg laying amount and Grasshopper Eggs collection method of improving is it is characterised in that comprise the following steps:
(1) rice seedlings are cultivated: rice paddy seed is soaked seed to showing money or valuables one carries unintentionally, the rice paddy seed having showed money or valuables one carries unintentionally is sprinkling upon the seedlings nursing plate of absorbent paper On, put in growth cabinet, temperature setting is 30 ~ 35 DEG C, humidity is 70%, and the photoperiod is 14l:10d, carries out accelerating germination;Sprout Afterwards, put into equipped with breeding in the plastic tub of field soil sample and growth cabinet 29 DEG C of temperature of setting, humidity is 70%, and the photoperiod is 14l:10d, adds water in right amount, treats that Oryza sativa L. length, to 20-25cm, obtains rice seedlings, standby;
(2) build locust feeding environment: locust rearging cage is positioned in growth cabinet, the temperature setting of growth cabinet is 28 DEG C, humidity is 70%, and the photoperiod is 14l:10d, and places the box body of open-like shape in locust rearging cage, and box body is built with confession The quartz sand of locust spawning, and the humidity of holding quartz sand is 30%;
(3) raise locust: the locust adult of field acquisition is placed in rearging cage and is raised, step (1) is obtained simultaneously Rice seedlings are put in rearging cage and are gnawed for locust, and rice seedlings are changed in timing;
(4) Grasshopper Eggs are collected: locust produces ovum in quartz sand, is covered with taking-up box body after quartz sand after pieces of an egg, adds in box body Water, locust pieces of an egg are washed in a pan out, and the pieces of an egg after washing in a pan out put into Refrigerator store, and storage temperature is 4 DEG C.
2. according to claim 1 a kind of improve locust egg laying amount and Grasshopper Eggs collection method it is characterised in that: step (1) in, plastic tub is long 40cm, wide 30cm, a height of 12cm.
3. according to claim 1 a kind of improve locust egg laying amount and Grasshopper Eggs collection method it is characterised in that: step (2), in, the size of rearging cage is 45cm, high 60cm for length and width.
4. according to claim 1 a kind of improve locust egg laying amount and Grasshopper Eggs collection method it is characterised in that: step (2) in, box body is long 25cm, wide 14cm, the plastic casing of a height of 5cm.
CN201610721648.7A 2016-08-25 2016-08-25 Method for improving locust egg laying amount and for collecting locust eggs Pending CN106359306A (en)

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Cited By (10)

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US10638788B2 (en) 2016-05-04 2020-05-05 Aspire Food Group USA Inc. Method and system for spray drying insects
US11026439B2 (en) 2016-05-04 2021-06-08 Aspire Food Group Ltd Method and system for spray drying insects
US11395474B2 (en) 2016-05-10 2022-07-26 Aspire Food Group Ltd Harvesting and incubating systems for cultivation of insects
USD841898S1 (en) 2017-04-13 2019-02-26 Aspire Food Group USA Inc. Insect habitat stacker
CN107136025A (en) * 2017-04-27 2017-09-08 马山县誉原生态蝗虫养殖专业合作社 Locust feeder
CN107318788A (en) * 2017-08-21 2017-11-07 安龙县海子镇祥飞农业综合开发有限公司 A kind of cultural method of locust
CN107897130A (en) * 2017-12-11 2018-04-13 四川省农业科学院植物保护研究所 A kind of locust artificial feeding and the collection method of Grasshopper Eggs
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CN110800697A (en) * 2019-11-25 2020-02-18 山东农业大学 A method for increasing the number of eggs laid by a single female of East Asian locust
CN111493029A (en) * 2020-04-28 2020-08-07 浙江农林大学 A kind of breeding method of yellow-spine bamboo locust

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