CN1060909C - Artificial seed and preparation method thereof - Google Patents
Artificial seed and preparation method thereof Download PDFInfo
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- CN1060909C CN1060909C CN96103269A CN96103269A CN1060909C CN 1060909 C CN1060909 C CN 1060909C CN 96103269 A CN96103269 A CN 96103269A CN 96103269 A CN96103269 A CN 96103269A CN 1060909 C CN1060909 C CN 1060909C
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- endosperm
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Abstract
本发明属于无性繁殖植株领域；特别是属于人工合成种子技术。把人工种子胚乳分成含有不同营养成份或植物生长调节剂的两部分，其两部分的结合部为藻酸的金属络合物层，这样的人工种子由于胚乳中含有强度较弱的结合面，不仅有利于不定胚芽的萌发和生长，排除了根和芽生长调节剂相混而分别对芽和根的抑制作用，可以减少生长调节剂的用量，而且避免了一般人工种子制备技术所存在的不含胚体或含多个胚体的人工种子的形成，以保证每个人工种子都只含一个胚体，且设备简单，降低成本。The invention belongs to the field of asexually propagated plants; in particular, it belongs to the artificially synthesized seed technology. The endosperm of the artificial seed is divided into two parts containing different nutrients or plant growth regulators, and the joint part of the two parts is a metal complex layer of alginic acid. Such an artificial seed has a weaker joint surface in the endosperm, not only It is beneficial to the germination and growth of adventitious germs, eliminates the inhibition of root and shoot growth regulators on buds and roots, can reduce the amount of growth regulators, and avoids the common artificial seed preparation technology that does not contain Embryo bodies or artificial seeds containing multiple embryo bodies are formed to ensure that each artificial seed contains only one embryo body, and the equipment is simple and the cost is reduced.
Description
The invention belongs to vegetative propagation plant field, particularly belong to synthetic seed technology.
Artificial seed is formed with suitable medium parcel back by adventitious embryo of plant or indefinite bud, as to sprout and be grown to normal plant synthetic seed.Parcel adventitious embryo or the used dielectric material of indefinite bud generally are spawns, and it includes for idiosome and blastogenesis long necessary nutrient, moisture and growth regulator.Because such medium has played and has been similar to endosperm role in the natural seed, therefore in artificial seed, be called artificial endosperm.Artificial endosperm also must have suitable intensity except that need contain certain constituent, idiosome and bud in can both guaranteeing to be comprised in are not mechanically damaged, and can not hinder the sprouting and the growth of idiosome and bud again.Because idiosome and the bud of plant are all very tender and lovely, both thermo-labile and cold-resistant, the not stimulation of chemically-resistant material again, therefore for as the dielectric material of artificial endosperm and the condition when wrapping up idiosome or bud the requirement and the restriction of strictness are all arranged.The alginates natural polysaecharides material that to be classes extract from marine alga (brown alga), the alginates of monovalence metal easily are dissolved in water, but with the polyvalent metal complexing after it can become hydrogel soon.Idiosome of alginates hydrogel and plant and bud have fabulous compatibility, so alginates are current general in the world artificial endosperm host materials, form calcium alginate gel by the mosanom aqueous solution is splashed into calcium chloride solution.
Current, the preparation method of artificial seed has two kinds basically.Clear and 60-214811 and clear and 63-5009111 have reported elder generation adventitious embryo or the indefinite bud of plant have been sneaked into solution of sodium alginate, and then this solution of sodium alginate that is mixed with adventitious embryo or indefinite bud is splashed into calcium chloride solution, make its gelation.Another kind is that solution of sodium alginate and plant idiosome (or bud) are transported from two pipelines respectively, at pipeline exit the two is mixed and also together splashes into the calcium chloride solution gelation.Their common drawback all is that employed growth regulator to root and bud mixes, and can play inhibitory action respectively to the growth of bud and root; In addition, because adventitious embryo (bud) is wrapped in the spawn of one deck performance homogeneous, the sprouting and the growth of adventitious embryo (bud) there is certain inhibition.
The objective of the invention is to solve the growth regulator to root and bud that current artificial seed exists mixes, the bud of adventitious embryo (bud) and the growth of root are caused inhibitory action on the contrary, and the inhibition that the endosperm structure of whole homogeneous is sprouted adventitious embryo (bud) and growth is risen.
Preparation method proposed by the invention has the artificial endosperm structure that two hemisphere are formed, owing to have the weak interface of the whole other parts of a strength ratio between two hemisphere, therefore can help the sprouting of artificial seed.The long conditioning agent of short root growth and short blastogenesis is added in utilization respectively in 2 half parts of artificial endosperm, can promote the growth of root, bud respectively, produce thereby the conditioning agent of avoiding general root, bud mixes respectively to the inhibitory action of bud, root growth, thereby can shorten the sprouting cycle.
The present invention prepares the technology of artificial seed, and not only equipment is simple, and is with low cost, but also can avoid the existing existing formation that does not contain idiosome or contain the artificial seed of a plurality of idiosomes of artificial seed technology of preparing, all only contains an idiosome to guarantee each artificial seed.When the preparation artificial seed, carry out according to the following steps:
The first step adopts one to contain a hole or porous cave, pore size appropriate template by the preparation demand, and injecting one in the hole contains the endosperm matrix solution A that required growth regulator is grown in the root or the blastogenesis of adventitious embryo or indefinite bud;
In second step,, reacted 1-5 minute dripping the metal chloride solutions that concentration is 1-10% on the liquid level of endosperm matrix solution A;
In the 3rd step, on every endosperm matrix solution A, splash into an adventitious embryo or indefinite bud;
In the 4th step, on endosperm matrix solution A and adventitious embryo or indefinite bud, drip again and contain the bud of adventitious embryo or indefinite bud or the endosperm matrix solution B of the required growth regulator of root growth;
The 5th step was the CaCl of 2-5% dripping the foraminous die plate immersion concentration that endosperm matrix and adventitious embryo or indefinite bud are arranged
2In the solution, react and pull out after 10-30 minute, use MS medium rinsing 2 minutes again, finally obtain endosperm and be made up of the two parts that contain Different Nutrition composition or plant growth regulator respectively, two-part joint portion is the artificial seed of the metal complex layer of alginic acid.
The artificial seed that the present invention prepares, the interface portion of its endosperm two hemispheres part are the complex compound of the metal complex layer, particularly calcium of the above alginic acid of divalent beyond the demagging or divalent.It is that the metal chloride solutions of 1-10% and the reaction of solution of sodium alginate that concentration is 1-10% form by concentration expressed in percentage by weight.The composition of endosperm matrix solution is: H
2O, mosanom, plant growth regulator, biological sterilization agent, nutrition, porous aggregate or other modifier etc.Endosperm matrix A is meant; If contain the growth regulator of root is not just contained growth regulator to bud, and this moment, endosperm matrix B only contained growth regulator to bud; Otherwise endosperm matrix A contains the growth regulator to bud, and endosperm matrix B only contains the growth regulator to root simultaneously.
Using the present invention to prepare in the process of artificial seed,, can above foraminous die plate, install and plumule distribution plate position consistency in order to keep the mechanization of preparation work, but one of mutual alternative group or several groups of water droppers again, respectively in order to drip the endosperm matrix solution.The pore size of selected foraminous die plate and plumule distribution plate can be decided by the size of various indefinite plumules.Regulate and distribute plate to the distance between foraminous die plate to guarantee that under the freely falling body condition selected indefinite plumule can both reach foraminous die plate with same direction upside down.
The artificial seed that adopts the present invention to prepare, owing to have the artificial endosperm structure of being made up of two hemisphere, the bond strength at interface is lower between two hemisphere, so help the sprouting and the growth of adventitious embryo (bud).When the preparation artificial seed, can be regulated respectively according to the root of different adventitious embryos (bud) and the required growth regulator of bud and nutrition, so not only can get rid of the long conditioning agent of root and blastogenesis respectively to the inhibitory action of bud and root growth, can save plant growth regulator, and can be owing to containing the more weak combination interface of intensity in the endosperm, the growth of adventitious embryo (bud) sprouted reduce resistance.
Embodiment:
Get each 2.5 gram of mosanom that contain 0.1 milligram of root and the long conditioning agent of blastogenesis respectively, little porous calcium alginate filler 0.5 of handling through Biodegradable high molecular restrains, biological sterilization agent 0.01 gram, under high-speed stirred, inject the container that contains 100 gram MS medium respectively, stirred 3 minutes, and made artificial endosperm matrix solution A and B.Logical tawny daylily stem segment is as the parcel object.
In the hole of giving the foraminous die plate for preparing earlier, splash into an endosperm matrix solution A, and then to drip concentration be 3% CaCl
2Solution after 2 minutes, splashes into logical tawny daylily stem segment, drips endosperm matrix solution B again; To drip the foraminous die plate that endosperm matrix and stem segment are arranged subsequently, to immerse concentration be 3% CaCl
2In the solution, react and pull out after 10 minutes, use MS medium rinsing 2 minutes again, wipe dry gets the logical tawny daylily artificial seed of the present invention's preparation.
Claims (3)
1, a kind of artificial seed, comprise adventitious embryo or indefinite bud and endosperm, it is characterized in that endosperm is divided into contains short root growth conditioning agent and contains short long conditioning agent two parts of blastogenesis, and two-part joint portion is the calcium complex layer that calcium chloride solution and solution of sodium alginate are reacted the alginic acid that forms.
2, a kind of artificial seed according to claim 1 is characterized in that described calcium chloride solution concentration is 1-10%.
3, the preparation method of a kind of artificial seed according to claim 1 is characterized in that:
(1) in the template hole of containing a hole or porous cave, splash into contain short root or contain the plant growth regulator endosperm matrix solution of short bud after, on its liquid level, drip last 1 calcium chloride solution that concentration is 1-10%, react after 1-5 minute, on liquid level, splash into 1 adventitious embryo or indefinite bud again
(2) on the above-mentioned endosperm matrix solution liquid level that contains adventitious embryo or indefinite bud, drip 1 plant growth regulator endosperm matrix solution that contains short root or contain short bud again,
(3) will drip a hole or porous cave template immersion concentration that endosperm matrix and adventitious embryo or indefinite bud are arranged is in the calcium chloride solution of 2-5%, reacts and pulls out after 10-30 minute, uses the rinsing of MS medium again, makes artificial seed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96103269A CN1060909C (en) | 1996-03-15 | 1996-03-15 | Artificial seed and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96103269A CN1060909C (en) | 1996-03-15 | 1996-03-15 | Artificial seed and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1148462A CN1148462A (en) | 1997-04-30 |
| CN1060909C true CN1060909C (en) | 2001-01-24 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN96103269A Expired - Fee Related CN1060909C (en) | 1996-03-15 | 1996-03-15 | Artificial seed and preparation method thereof |
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| Country | Link |
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| CN (1) | CN1060909C (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100403883C (en) * | 2006-05-11 | 2008-07-23 | 南京大学 | Method for making artificial seeds of clematis |
| CN101120655B (en) * | 2007-09-18 | 2010-11-17 | 华中师范大学 | Method for making artificial seeds of Smilax |
| CN101773073B (en) * | 2010-02-26 | 2012-05-30 | 肇庆学院 | Preparation method of artificial seeds of geranium purple back |
| CN108207534A (en) * | 2018-01-31 | 2018-06-29 | 上海应用技术大学 | The processing of state's orchid artificial endosperm seed with capsule and method for culturing seedlings |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH034706A (en) * | 1989-05-31 | 1991-01-10 | Kubota Corp | Preparation of artificial seed |
| JPH03127920A (en) * | 1989-10-11 | 1991-05-31 | Kubota Corp | How to make artificial seeds |
| JPH03206825A (en) * | 1990-01-08 | 1991-09-10 | Kubota Corp | Artificial seeds and their manufacturing method |
| JPH03285624A (en) * | 1990-03-30 | 1991-12-16 | Pentel Kk | Cultivation of plant tissue and artificial seed |
| WO1993003069A1 (en) * | 1991-08-07 | 1993-02-18 | Henkel Kommanditgesellschaft Auf Aktien | Polyacrylates and their use as levelling agents for powder coatings |
| EP0605721A1 (en) * | 1991-08-20 | 1994-07-13 | Kirin Beer Kabushiki Kaisha | Self-dehiscent artificial seed |
-
1996
- 1996-03-15 CN CN96103269A patent/CN1060909C/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH034706A (en) * | 1989-05-31 | 1991-01-10 | Kubota Corp | Preparation of artificial seed |
| JPH03127920A (en) * | 1989-10-11 | 1991-05-31 | Kubota Corp | How to make artificial seeds |
| JPH03206825A (en) * | 1990-01-08 | 1991-09-10 | Kubota Corp | Artificial seeds and their manufacturing method |
| JPH03285624A (en) * | 1990-03-30 | 1991-12-16 | Pentel Kk | Cultivation of plant tissue and artificial seed |
| WO1993003069A1 (en) * | 1991-08-07 | 1993-02-18 | Henkel Kommanditgesellschaft Auf Aktien | Polyacrylates and their use as levelling agents for powder coatings |
| EP0605721A1 (en) * | 1991-08-20 | 1994-07-13 | Kirin Beer Kabushiki Kaisha | Self-dehiscent artificial seed |
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| Publication number | Publication date |
|---|---|
| CN1148462A (en) | 1997-04-30 |
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