CN106035407B - A kind of vegetalitas culicifuge, preparation and its application of repellent aedes albopictus - Google Patents
A kind of vegetalitas culicifuge, preparation and its application of repellent aedes albopictus Download PDFInfo
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Abstract
Description
技术领域technical field
本发明涉及一种驱避白纹伊蚊的植物性驱蚊剂及其应用，属于植物源农药技术领域。The invention relates to a plant-based mosquito repellent for repelling Aedes albopictus and an application thereof, belonging to the technical field of plant-derived pesticides.
背景技术Background technique
白纹伊蚊（Aedes albopictus Skuse）又称亚洲虎蚊，是东南亚和中国的常见蚊种，一般在户外活动，其雌蚊吸食人和动物的血，是非常活跃和凶猛的吸血者，严重骚扰人类的正常生活，同时也是一种重要的病毒媒介，它可以传播多种疾病，如乙型脑炎、登革热等蚊媒传染病。 Aedes albopictus Skuse, also known as the Asian tiger mosquito, is a common species of mosquito in Southeast Asia and China. It generally lives outdoors. Its female mosquito sucks the blood of humans and animals. It is a very active and ferocious blood sucker, which seriously harasses people. The normal life of human beings is also an important virus vector, which can transmit a variety of diseases, such as Japanese encephalitis, dengue fever and other mosquito-borne infectious diseases.
人们在野外活动时，常使用各种驱蚊剂防止白纹伊蚊的骚扰、控制蚊媒疾病传播。目前普遍使用的驱蚊剂中驱蚊成分一般是以避蚊胺（DEET）为主，避蚊胺驱蚊虽然效果好，但是长期使用会造成对环境的污染，其毒副作用也不断有报道, 如出现神经系统症状、皮肤损害、引起儿童脑病、引起荨麻疹综合症、血压低和心跳降低等。而某些植物的次生代谢产物对白纹伊蚊蚊虫具有较好的驱避作用，利用其研制的驱蚊剂具有高效低毒或无毒、易降解对环境友好的特点，这些绿色环保的植物性驱蚊剂对人们在野外躲避蚊虫的叮咬避免蚊媒病的传播具有重要的实用价值。When people are active in the wild, they often use various mosquito repellents to prevent the harassment of Aedes albopictus and control the spread of mosquito-borne diseases. At present, the mosquito repellent ingredients in commonly used mosquito repellants are generally DEET. Although DEET is effective in repelling mosquitoes, long-term use will cause pollution to the environment, and its toxic and side effects are constantly reported. Such as nervous system symptoms, skin lesions, encephalopathy in children, urticarial syndrome, low blood pressure, and low heartbeat. And the secondary metabolites of some plants have good repellent effect on Aedes albopictus mosquitoes, and the mosquito repellent developed by using them has the characteristics of high efficiency, low toxicity or non-toxicity, easy degradation and environmental friendliness. These green and environmentally friendly plants Sexual mosquito repellents have important practical value for people to avoid mosquito bites and avoid the transmission of mosquito-borne diseases in the wild.
植物精油对蚊虫的驱避作用具有高效性和专一性，同一种植物精油对不同的蚊虫的驱避效果差异较大，因此，针对不同的防控对象筛选植物精油是开发植物性驱蚊产品的关键。The repelling effect of plant essential oils on mosquitoes is highly efficient and specific. The repelling effect of the same plant essential oil on different mosquitoes is quite different. Therefore, screening plant essential oils for different prevention and control objects is the key to developing plant-based mosquito repellent products. key.
发明内容Contents of the invention
本发明的目的是提供一种对人类无毒副作用对环境无污染的植物性驱蚊剂，用于驱避白纹伊蚊，具有显著的驱避效果。The object of the present invention is to provide a plant-based mosquito repellent which has no toxic side effects on human beings and no pollution to the environment, which is used for repelling Aedes albopictus and has a significant repelling effect.
本发明的技术方案为：一种驱避白纹伊蚊的植物性驱蚊剂，该驱蚊剂的主要活性物为：含有紫花香薷精油、椿叶花椒精油和一年蓬精油的组合物，该组合物中各组分的质量份含量为紫花香薷精油40~65份、椿叶花椒精油20~35份和一年蓬精油15~30份。The technical solution of the present invention is: a plant-based mosquito repellent for repelling Aedes albopictus, the main active substance of which is: a composition containing essential oils of A. The mass parts content of each component in the composition is 40-65 parts of the essential oil of the cypress essential oil, 20-35 parts of the essential oil of the toon leaf prickly ash, and 15-30 parts of the essential oil of the annual prickly ash.
所述紫花香薷精油、椿叶花椒精油和一年蓬精油是通过水蒸汽蒸馏法分别从紫花香薷全草、椿叶花椒果实和一年蓬花中提取得到。The essential oil of Arthia sativa, the essential oil of Zanthoxylum chinensis and the essential oil of Zanthoxylum chinensis are respectively extracted from the whole herb of Zanthoxylum sativa, the fruit of Zanthoxylum chinensis and the flowers of Annus chinensis through a steam distillation method.
所述组合物还包括溶剂，溶剂占所述组合物总重量的5~10%。The composition also includes a solvent, and the solvent accounts for 5-10% of the total weight of the composition.
在一个具体实施方式中，该驱蚊剂为霜剂，所述组合物占该霜剂的质量百分比含量为60~80%。驱蚊霜还包括乳化剂1～4%、滋润剂10～25%、保湿剂1～6%、抗刺激剂0.1～1%、pH调节剂0.1～0.5%、防腐剂0.01～0.06%、赋香剂0.1～0.6%，其余为蒸馏水，合计100%，以占该霜剂的质量百分比计。In a specific embodiment, the mosquito repellent is a cream, and the composition accounts for 60-80% by mass of the cream. Mosquito repellent cream also includes 1-4% emulsifier, 10-25% moisturizer, 1-6% moisturizer, 0.1-1% anti-irritant, 0.1-0.5% pH regulator, 0.01-0.06% preservative, Fragrance 0.1-0.6%, the rest is distilled water, the total is 100%, calculated by the mass percentage of the cream.
在一个具体实施方式中，该驱蚊剂为喷雾剂，所述组合物占该喷雾剂的质量百分比含量为50～65%。喷雾剂还包括乳化剂1～5%、滋润剂1～4%、保湿剂1～10%、抗刺激剂0.1～1%、pH调节剂0.1～0.5%、防腐剂0.01～0.1%、赋香剂0.1～0.6%、其余为蒸馏水，合计100%，以占该喷雾剂的质量百分比计。In a specific embodiment, the mosquito repellent is a spray, and the composition accounts for 50-65% by mass of the spray. The spray also includes 1-5% emulsifier, 1-4% moisturizer, 1-10% moisturizer, 0.1-1% anti-irritant, 0.1-0.5% pH regulator, 0.01-0.1% preservative, fragrance 0.1-0.6% of the spray, and the rest is distilled water, the total is 100%, calculated as the mass percentage of the spray.
所述乳化剂为聚氧乙烯醚类、羧酸盐、硫酸盐、聚氧丙烯醚类和磺酸盐中的一种或以上，所述滋润剂为异构十二烷、高级醇、动植物油和合成油脂中的一种或以上，保湿剂为多元醇、透明质酸和氨基酸中的一种或以上，所述抗刺激剂为α-红没药醇，所述pH调节剂为三乙醇胺或柠檬酸。The emulsifier is one or more of polyoxyethylene ethers, carboxylates, sulfates, polyoxypropylene ethers and sulfonates, and the wetting agent is isomerized dodecane, higher alcohols, animal and vegetable oils and one or more of synthetic oils, the moisturizing agent is one or more of polyols, hyaluronic acid and amino acids, the anti-irritant is α-bisabolol, and the pH regulator is triethanolamine or citric acid.
上述植物性驱蚊剂的制备方法，是将各原料混合均匀、乳化、均质制成。The preparation method of the above-mentioned plant-based mosquito repellent is prepared by uniformly mixing, emulsifying and homogenizing all raw materials.
本发明的植物性驱避剂，由紫花香薷精油、椿叶花椒精油和一年蓬精油组成的组合物，配入助剂配制成驱蚊霜、驱蚊喷雾剂，在室外对白纹伊蚊具有显著的驱避效果，且对人畜安全无毒害，对环境不造成污染。至今为止尚未见由紫花香薷精油、椿叶花椒精油和一年蓬精油组合制成的植物性驱蚊剂。The botanical repellent of the present invention is a composition composed of Aurantia japonica essential oil, Chinese prickly ash essential oil and annual prickly ash essential oil, mixed with auxiliary agents to make mosquito repellent cream and mosquito repellent spray, which has the effect on Aedes albopictus outdoors Significant repelling effect, safe and non-toxic to humans and animals, and does not cause pollution to the environment. So far, there is no plant-based mosquito repellent made from the combination of Aurantia chinensis essential oil, Toon leaf Zanthoxylum essential oil and annual plant essential oil.
具体实施方式Detailed ways
本发明的植物性驱蚊剂主要活性物为：含有紫花香薷精油、椿叶花椒精油和一年蓬精油的组合物（下述的“植物性驱蚊剂组合物”），该组合物中各组分的质量份含量为紫花香薷精油40~65份、椿叶花椒精油20~35份和一年蓬精油15~30份。制备时用无水乙醇分别对紫花香薷精油、椿叶花椒精油和一年蓬精油溶解，然后混合即得，其中无水乙醇占驱蚊剂组合物重量的5~10%。The main active substance of the plant-based mosquito repellent of the present invention is: a composition containing the essential oil of Aurantia japonica, essential oil of Chinese toon leaf Zanthoxylum bungeanum, and essential oil of Annus chinensis (hereinafter referred to as "plant-based mosquito repellent composition"), in which each The parts by mass of the components are 40-65 parts of the essential oil of Aurantia chinensis, 20-35 parts of the essential oil of Zanthoxylum bungeanum, and 15-30 parts of the essential oil of P. annua. During preparation, anhydrous ethanol is used to respectively dissolve the essential oils of Aurantia japonica, essential oil of Chinese prickly ash and annua essential oil, and then mix them to obtain the product, wherein the absolute ethanol accounts for 5-10% of the weight of the mosquito repellent composition.
所述的紫花香薷精油由唇形科（Labiatae）紫花香薷（Elsholtzia argyi）全草经水蒸汽蒸馏而得。所述的椿叶花椒精油由芸香科（Rutaceae）椿叶花椒（Zanthoxylum ailanthoides）果实用水蒸汽蒸馏而得。所述的一年蓬精油由菊科（Erigeron）植物（Erigeron annuus）花序用水蒸汽蒸馏而得。所述水蒸汽蒸馏法采用常规的共水蒸馏。The essential oil of Elsholtzia argyi is obtained by steam distillation from the whole herb of Elsholtzia argyi of Labiatae. The Zanthoxylum essential oil is obtained by steam distillation from the fruit of Zanthoxylum ailanthoides of Rutaceae. The annuncia essential oil is distilled from inflorescences of Erigeron annuus ( Erigeron annuus ) with water steam. The steam distillation method adopts conventional co-water distillation.
根据需要，可以将植物性驱蚊剂制成霜剂或喷雾剂等形式。Plant-based repellents are available in the form of creams or sprays as needed.
在一个具体实施方式中，驱蚊霜包括下述组分（下述质量百分比以驱蚊霜整体为基准）：植物性驱蚊剂组合物60~80%、乳化剂1～4%、滋润剂10～25%、保湿剂1～6%、抗刺激剂0.1～1%、pH调节剂0.1～0.5%、防腐剂0.01～0.06%、赋香剂0.1～0.6%。In a specific embodiment, the mosquito repellent cream includes the following components (the following mass percentages are based on the whole mosquito repellent cream): plant-based mosquito repellent composition 60-80%, emulsifier 1-4%, moisturizer 10-25%, humectant 1-6%, anti-irritant 0.1-1%, pH regulator 0.1-0.5%, preservative 0.01-0.06%, flavoring agent 0.1-0.6%.
制备时将上述原料按比例混合均匀，以蒸馏水加至100%，进行乳化，然后均质5～8分钟，即制备成驱蚊霜。然后取样对制剂的稳定性与外观进行检测，合格后包装，即得成品。During preparation, the above-mentioned raw materials are mixed evenly in proportion, added to 100% with distilled water, emulsified, and then homogenized for 5-8 minutes to prepare the mosquito repellent cream. Then take a sample to test the stability and appearance of the preparation, and pack it after passing the test to obtain the finished product.
在一个具体实施方式中，驱蚊喷雾剂包括下述组分（下述质量百分比以喷雾剂整体为基准）：植物性驱蚊剂组合物50～65%、乳化剂1～5%、滋润剂1～4%、保湿剂1～10%、抗刺激剂0.1～1%、PH调节剂0.1～0.5%、防腐剂0.01～0.1%、赋香剂0.1～0.6%、其余为蒸馏水。In a specific embodiment, the mosquito repellent spray includes the following components (the following mass percentages are based on the spray as a whole): plant-based mosquito repellent composition 50-65%, emulsifier 1-5%, moisturizer 1-4%, moisturizer 1-10%, anti-irritant 0.1-1%, PH regulator 0.1-0.5%, preservative 0.01-0.1%, flavoring agent 0.1-0.6%, and the rest is distilled water.
制备时将上述原料按比例混合均匀，以蒸馏水加至100%，进行乳化，然后均质3～5min，即制备成驱蚊喷雾剂。然后取样对制剂的稳定性与外观进行检测，合格后包装，即得成品。During preparation, the above raw materials are mixed uniformly in proportion, added to 100% with distilled water, emulsified, and then homogenized for 3-5 minutes to prepare the mosquito repellent spray. Then take a sample to test the stability and appearance of the preparation, and pack it after passing the test to obtain the finished product.
以下实施例对本发明做进一步的说明，而不是限制本发明的保护范围。The following examples further illustrate the present invention, but do not limit the protection scope of the present invention.
实施例1、驱蚊霜的制备及室外驱避白纹伊蚊实验Example 1, Preparation of Mosquito Repellent Cream and Experiment of Repelling Aedes Albopictus Outdoors
驱蚊霜由以下方法制得：取本发明植物性驱蚊剂组合物质量百分比为75%、乳化剂2%、滋润剂15%、保湿剂6%、抗刺激剂0.5%、pH调节剂0.25%、防腐剂0.05%、赋香剂0.2%。将上述原料按比例混合均匀，以蒸馏水加至100%，抽入乳化锅进行乳化，然后均质5～8 分钟，即制备成驱蚊霜，然后取样对制剂的稳定性与外观进行检测，合格后包装，即得成品。Mosquito repellent cream is prepared by the following method: take the plant-based mosquito repellent composition of the present invention as 75% by mass, 2% emulsifier, 15% moisturizer, 6% moisturizer, 0.5% anti-irritant, and 0.25% pH regulator. %, preservative 0.05%, flavoring agent 0.2%. Mix the above raw materials evenly in proportion, add distilled water to 100%, pump into the emulsification pot for emulsification, and then homogenize for 5 to 8 minutes to prepare mosquito repellent cream, then take samples to test the stability and appearance of the preparation, and it is qualified After packaging, the finished product is obtained.
以上所述本发明植物性驱蚊剂组合物由紫花香薷精油质量百分比50%、椿叶花椒精油25%、一年蓬精油20%，加入5%的乙醇溶解混合而成。以上所述乳化剂为聚氧乙烯醚类，所述滋润剂为合成油脂，所述氨基酸，所述抗刺激剂为α-红没药醇，所述pH调节剂为柠檬酸。The plant-based mosquito repellent composition of the present invention described above is composed of 50% by mass percentage of the essential oil of Aurantia chinensis, 25% of the essential oil of Prickly ash, and 20% of the essential oil of Annua, dissolved and mixed by adding 5% ethanol. The above emulsifier is polyoxyethylene ether, the moisturizer is synthetic oil, the amino acid, the anti-irritant is α-bisabolol, and the pH regulator is citric acid.
实验地点在中南林业科技大学竹林，此地段白纹伊蚊数量多攻击力强；实验时间为7月中下旬每天日落前的3个小时，此时白纹伊蚊的攻击性最强，试验设3次重复；每次实验由4位志愿者完成，使用驱蚊霜前，测得每5 min内蚊虫对4位志愿者的攻击只数平均为4只。将配制好的驱蚊霜均匀涂抹于四位志愿者的胳膊及腿的裸露部位，剂量为刚好覆盖裸露皮肤为止。之后志愿者可在实验点范围内正常活动或静坐，测得驱蚊霜对志愿者的100%完全保护时间平均值为5.65 ± 0. 19 h。The experimental site is in the bamboo forest of Central South University of Forestry and Technology, where the number of Aedes albopictus is large and the attack power is strong; the experimental time is 3 hours before sunset every day in the middle and late July, when the attack of Aedes albopictus is the strongest. Repeated 3 times; each experiment was completed by 4 volunteers. Before using the mosquito repellent cream, the average number of mosquitoes attacking the 4 volunteers every 5 minutes was 4. The prepared mosquito repellent cream was evenly applied to the bare parts of the arms and legs of four volunteers, and the dose was just enough to cover the bare skin. Afterwards, the volunteers could move normally or sit quietly within the range of the experimental point, and the average time of 100% complete protection of the volunteers by the mosquito repellent cream was measured to be 5.65 ± 0.19 h.
实施例2、驱蚊喷雾剂的制备及室外驱避实验Embodiment 2, preparation of mosquito repellent spray and outdoor repellent experiment
实验用的驱蚊喷雾剂由以下方法制得：取本发明植物性驱蚊剂组合物质量百分比为60%、乳化剂4%、滋润剂2%、保湿剂4%、抗刺激剂0.5%、pH调节剂0.35%、防腐剂0.05%、赋香剂0.1%、其余为蒸馏水。制造时将上述原料按比例混合均匀，以蒸馏水加至100%，抽入乳化锅进行乳化，然后均质3～5 min，即制备成驱蚊喷雾剂。然后取样对制剂的稳定性与外观进行检测，合格后包装，即得成品。The mosquito repellent spray used in the experiment was prepared by the following method: take the plant mosquito repellent composition mass percentage of the present invention as 60%, emulsifier 4%, moisturizer 2%, moisturizing agent 4%, anti-irritant 0.5%, 0.35% pH adjuster, 0.05% preservative, 0.1% flavoring agent, and the rest is distilled water. When manufacturing, the above raw materials are mixed evenly in proportion, added to 100% with distilled water, pumped into the emulsification pot for emulsification, and then homogenized for 3 to 5 minutes to prepare the mosquito repellent spray. Then take a sample to test the stability and appearance of the preparation, and pack it after passing the test to obtain the finished product.
以上所述本发明植物性驱蚊剂组合物由紫花香薷精油质量百分比50%、椿叶花椒精油25%、一年蓬精油20%，加入5%的乙醇溶解混合而成。以上所述乳化剂为聚氧乙烯醚类，所述滋润剂为合成油脂，所述氨基酸，所述抗刺激剂为α-红没药醇，所述pH调节剂为柠檬酸。The plant-based mosquito repellent composition of the present invention described above is composed of 50% by mass percentage of the essential oil of Aurantia chinensis, 25% of the essential oil of Prickly ash, and 20% of the essential oil of Annua, dissolved and mixed by adding 5% ethanol. The above emulsifier is polyoxyethylene ether, the moisturizer is synthetic oil, the amino acid, the anti-irritant is α-bisabolol, and the pH regulator is citric acid.
实验地点在中南林业科技大学竹林，此地段白纹伊蚊数量多攻击力强；实验时间为7月中下旬每天日落前的3个小时，此时白纹伊蚊的攻击性最强，试验设3次重复；每次实验由4位志愿者完成，使用驱蚊喷雾剂前，测得每5 min内蚊虫对4位志愿者的攻击只数平均为4只。将配制好的驱蚊喷雾剂均匀喷洒于志愿者四肢的裸露皮肤，剂量为刚好覆盖裸露皮肤为止。之后志愿者可在实验点范围内正常活动或静坐，测得驱蚊喷雾剂对志愿者的100%完全保护时间平均值为4.36± 0.21 h。The experimental site is in the bamboo forest of Central South University of Forestry and Technology, where the number of Aedes albopictus is large and the attack power is strong; the experimental time is 3 hours before sunset every day in the middle and late July, when the attack of Aedes albopictus is the strongest. Repeated 3 times; each experiment was completed by 4 volunteers. Before using the mosquito repellent spray, the average number of mosquitoes attacking the 4 volunteers every 5 minutes was 4. The prepared mosquito repellent spray was evenly sprayed on the bare skin of the volunteers' limbs, and the dose was just enough to cover the bare skin. Afterwards, the volunteers could move normally or sit quietly within the range of the experimental point, and the average time of 100% complete protection of the volunteers by the mosquito repellent spray was measured to be 4.36±0.21 h.
实施例3Example 3
按实施例1的方法制备驱蚊霜，并测试驱避效果。所不同的是各组分含量：植物性驱蚊剂组合物质量百分比为60%、乳化剂3%、滋润剂10%、保湿剂4%、抗刺激剂0.3%、pH调节剂0.4%、防腐剂0.05%、赋香剂0.3%。植物性驱蚊剂组合物：紫花香薷精油质量百分比40%、椿叶花椒精油30%、一年蓬精油25%、乙醇5%。The mosquito repellent cream was prepared according to the method of Example 1, and the repellent effect was tested. The difference is the content of each component: the mass percentage of the plant-based mosquito repellent composition is 60%, emulsifier 3%, moisturizer 10%, moisturizer 4%, anti-irritant 0.3%, pH regulator 0.4%, antiseptic Agent 0.05%, flavoring agent 0.3%. The plant-based mosquito repellent composition: the mass percentage of the essential oil of Aurantia chinensis 40%, the essential oil of Chinese toon leaf prickly ash 30%, the essential oil of annual perch 25%, and 5% ethanol.
测试结果：对志愿者的100%完全保护时间平均值为4.13 ± 0.22 h。Test results: The average time of 100% complete protection for volunteers is 4.13 ± 0.22 h.
按本实施例的方法，分别用紫花香薷、椿叶花椒、一年蓬精油取代本实施例配方中的植物性驱蚊剂组合物，制备出三种单剂精油驱蚊霜，并测试驱蚊效果。得到的测试结果是：紫花香薷、椿叶花椒、一年蓬精油对志愿者的100%完全保护时间平均值分别为3.26 ±0.13 h、2.13 ± 0.17 h、1.98 ± 0.23 h。According to the method of this example, three kinds of single-dose essential oil mosquito repellent creams were prepared and tested for mosquito repellent by replacing the botanical mosquito repellent composition in the formula of this example with the essential oils of Aurantia chinensis, Toon leaf Zanthoxylum bungeanum, and Annus chinensis. Effect. The test results obtained are: the average time for 100% complete protection of the volunteers by the essential oils of Zihuazi, Zanthoxylum bungeanum, and Nianneng are 3.26 ± 0.13 h, 2.13 ± 0.17 h, and 1.98 ± 0.23 h, respectively.
测试结果表明，由本发明植物性驱蚊剂组合物制备的驱蚊霜比分别使用组合物中任何单一精油制备的驱蚊霜，驱避效果明显增强。The test results show that the repellent effect of the mosquito repellent cream prepared from the botanical mosquito repellent composition of the present invention is significantly stronger than that of the mosquito repellent cream prepared by using any single essential oil in the composition.
实施例4Example 4
按实施例2的方法制备驱蚊喷雾剂，并测试驱避效果。所不同的是各组分含量：植物性驱蚊剂组合物质量百分比为50%、乳化剂2%、滋润剂3%、保湿剂6%、抗刺激剂0.3%、pH调节剂0.2%、防腐剂0.05%、赋香剂0.2%。植物性驱蚊剂组合物：紫花香薷精油质量百分比60%、椿叶花椒精油20%、一年蓬精油15%、乙醇5%。The mosquito repellent spray was prepared according to the method of Example 2, and the repellent effect was tested. The difference is the content of each component: the mass percentage of the plant-based mosquito repellent composition is 50%, emulsifier 2%, moisturizer 3%, moisturizer 6%, anti-irritant 0.3%, pH regulator 0.2%, antiseptic Agent 0.05%, flavoring agent 0.2%. The plant-based mosquito repellent composition: the mass percentage of the essential oil of Aurantia chinensis 60%, the essential oil of Chinese toon leaf prickly ash 20%, the essential oil of annenzae essential oil 15%, and 5% ethanol.
测试结果：对志愿者的100%完全保护时间平均值为3.25 ± 0.08h。Test results: The average time of 100% complete protection for volunteers is 3.25 ± 0.08h.
按本实施例的方法，分别用紫花香薷、椿叶花椒、一年蓬精油取代本实施例配方中的植物性驱蚊剂组合物，制备出三种单剂精油驱蚊喷雾剂，并测试驱蚊效果。得到的测试结果是：由紫花香薷、椿叶花椒、一年蓬精油制备的驱蚊喷雾剂对志愿者的100%完全保护时间平均值分别为2.89 ± 0.10 h、2.01 ± 0.17 h、1.78 ± 0.23 h。According to the method of this example, three kinds of single-dose essential oil mosquito repellent sprays were prepared, and tested to repel Mosquito effect. The test results obtained are: the average 100% complete protection time of the mosquito repellent spray prepared from the essential oils of Zihuazi, Toon leaf Zanthoxylum, and Annua is 2.89 ± 0.10 h, 2.01 ± 0.17 h, 1.78 ± 0.23 h, respectively. h.
测试结果表明，由本发明植物性驱蚊剂组合物制备的驱蚊喷雾剂比分别使用组合物中任何单一精油制备的驱蚊喷雾剂，对白纹伊蚊的驱避效果明显增强。The test results show that the mosquito repellent spray prepared by the plant-based mosquito repellent composition of the present invention has significantly stronger repelling effect on Aedes albopictus than the mosquito repellent spray prepared by using any single essential oil in the composition.
总结本发明：To summarize the invention:
经过发明人的反复试验，发现将紫花香薷精油、椿叶花椒精油和一年蓬精油按一定的比例复配得到的组合物，对白纹伊蚊具有显著的驱避作用。本发明选择的三种植物精油中各自存在多种类型的驱避化合物，如紫花香薷精油中含有芳樟醇、柠檬醛、苧烯、桉叶油醇等，椿叶花椒中含有蒎烯、桧烯、柠檬烯、2 壬酮 、蒈烯、萜烯醇、2 十一酮、癸酸乙烯等；一年蓬精油中含有β-榄香烯、大根香叶烯D、β-蛇床烯、金合欢烯等活性物质。三种植物精油混配后，活性成分之间药理上相互促进。植物精油化学成分复杂，并以不同的作用机制发挥作用，害虫难以产生抗药性，同时还具有易降解无残留、对人畜及其它非靶标生物安全等许多优势，同时本发明采用的助剂都为环保型助剂，对人体安全无毒对环境友好，且制备方法简单，实施简便。After repeated tests by the inventor, it is found that the composition obtained by compounding the essential oil of Aurantia japonica, the essential oil of Zanthoxylum bungeanum leaf and the essential oil of Annua in a certain proportion has a significant repelling effect on Aedes albopictus. There are multiple types of repelling compounds in the three kinds of plant essential oils selected by the present invention, such as linalool, citral, limonene, eucalyptol, etc. ene, limonene, 2-nonanone, carene, terpene alcohol, 2-undecone, vinyl caprate, etc.; the essential oil of anennea contains β-elemene, germanene D, β-osthole, gold Farnesene and other active substances. After the three plant essential oils are mixed, the active ingredients promote each other pharmacologically. The chemical composition of plant essential oil is complex, and it plays a role with different mechanisms of action. It is difficult for pests to develop drug resistance. At the same time, it also has many advantages such as easy degradation without residue, safety to humans, animals and other non-target organisms. At the same time, the additives used in the present invention are all The environment-friendly additive is safe, non-toxic and friendly to the environment, and has a simple preparation method and simple implementation.
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