CN106011045A - Method for preparing Sb-polluted site efficient ecological restoration flora by utilizing biogas residues - Google Patents

Abstract

The invention provides a method for using biogas residues to prepare efficient ecological restoration flora of Sb(V) polluted sites. By adding carbon source and sulfate to biogas residues, the sulfate-reducing flora in biogas residues can grow rapidly and produce sulfide at the same time. Subsequent addition of antimonate consumes the sulfide that inhibits the growth of sulfate-reducing bacteria, and the presence of sulfide also greatly reduces the toxicity of antimonate to sulfate-reducing bacteria, and at the same time makes the cultured sulfate-reducing bacteria resistant to Sb performance improved.

Description

A method of using biogas residues to prepare efficient ecological restoration flora of Sb-contaminated sites

technical field

The invention provides a method for using biogas residues to prepare highly efficient ecological restoration flora of Sb-contaminated sites. By adding carbon sources and sulfates to biogas residues, the sulfate-reducing flora in biogas residues can grow rapidly and produce sulfide at the same time; Adding antimonate consumes sulfide that inhibits the growth of sulfate-reducing bacteria, and the presence of sulfide also greatly reduces the toxicity of antimonate to sulfate-reducing bacteria, and at the same time improves the Sb resistance of the cultured sulfate-reducing bacteria , which belongs to the field of environmental protection.

Background technique

Due to the random discharge of mining and metallurgical wastewater, a large amount of soil is polluted by Sb. Current methods of treating mercury-containing soils are flawed. The high temperature melting method is costly and consumes a lot of energy. The biological method has a long reaction cycle and is likely to cause secondary pollution by foreign biological agents. The effect of the curing method is unstable, and the curing agent is likely to cause secondary pollution to the soil. Patent No. 201510056406.6 introduces a solidification method for soil containing heavy metals, but this method uses a large amount of materials, and the cost is high. On the other hand, the solidification effect is unstable. For example, the added calcium oxide is prone to carbonation under natural conditions. This reduces the effect of alkaline curing.

Contents of the invention

Aiming at the deficiencies of the prior art, the present invention provides a method of using biogas residues to prepare efficient ecological restoration flora of Sb(V) polluted sites, by adding carbon source and sulfate to biogas residues, so that sulfate-reducing bacteria in biogas residues The group grows rapidly and produces sulfide at the same time; then adding antimonate consumes the sulfide that inhibits the growth of sulfate-reducing bacteria, and the presence of sulfide also greatly reduces the toxicity of antimonate to sulfate-reducing bacteria, and at the same time makes the cultured The Sb-resistant performance of sulfate-reducing bacteria was improved.

The operation steps are as follows:

(1) Mix biogas residue with a solution containing a certain amount of carbon source, add a certain amount of sulfate, and put it into a reactor for biochemical reaction. Control the content of dry biogas residue in the mixed solution to 0.1-20%, and the solid content to 0.5- 25%, the organic carbon content is 0.5-20%, the mass ratio of organic carbon/sulfate (calculated as SO4) is (1-50):1, and then reacted for 0.1-5 days;

(2) in the solution after step (1) reaction, add a certain amount of antimonate, control the mol ratio (1-50) of sulfate/antimonate: 1, the mass ratio of organic carbon/antimony (10- 100): 1, followed by 0-5 days of reaction;

(3) Repeatedly add antimonate to the solution 0-4 times, each time controlling the molar ratio of sulfate/antimonate (1-50):1, the mass ratio of organic carbon/antimony (10-100): 1. The reaction time is 0-5 days, and then high-efficiency ecological restoration flora can be obtained.

Compared with traditional antimony-containing soil treatment methods, this method has the following advantages:

1. The cultivated sulfate-reducing bacteria are indigenous flora, with strong natural tolerance, easy to reproduce on a large scale in the field, effectively reducing antimonate, and will not cause ecological problems;

2. The cultivation and acclimation cycle is greatly shortened, and it can be cultivated within 1 day at the fastest, and the economy is greatly improved;

3. While cultivating sulfate-reducing bacteria, the metabolite sulfide is produced, which greatly reduces the toxicity of the subsequently added antimonate to sulfate-reducing bacteria. At the same time, because the metabolite sulfide itself can inhibit sulfate-reducing bacteria, so This method not only rapidly cultivates sulfate-reducing bacteria, but also enhances its resistance to antimony;

4. Sb and sulfate-reducing bacteria metabolite sulfide form sulfide Sb precipitation, making Sb more stable in soil.

The specific implementation examples are as follows:

Example 1:

(1) Mix biogas residue with a solution containing a certain amount of carbon source, add a certain amount of sulfate, put it into a reactor for biochemical reaction, control the content of dry biogas residue in the mixed solution to 0.5%, and the solid content to 10%, The organic carbon content is 12%, and the mass ratio of organic carbon/sulfate is 5:1, followed by reaction for 1 day;

(2) in the solution after step (1) reaction, add a certain amount of antimonate, control the mol ratio (2-3) of vitriol/antimonate: 1, the mass ratio of organic carbon/antimony (10- 30): 1, followed by reaction for 5 days;

(3) Repeatedly adding antimonate 2 times in the solution, each time controlling the mol ratio (2-3) of sulfate/antimonate: 1, the mass ratio of organic carbon/antimony (10-30): 1, The reaction time was 5 days, and then the highly efficient ecological restoration flora was obtained.

Example 2:

(1) Mix biogas residue with a solution containing a certain amount of carbon source, add a certain amount of sulfate, and put it into a reactor for biochemical reaction. Control the content of dry biogas residue in the mixed solution to be 8-10%, and the solid content to be 10- 15%,, the organic carbon content is 10-15%, the mass ratio of organic carbon/sulfate is 20:1, and then reacted for 0.1-1 day;

(2) in the solution after step (1) reaction, add a certain amount of antimonate, control the mol ratio (1-5) of vitriol/antimonate: 1, the mass ratio of organic carbon/antimony (30- 80): 1, followed by reaction for 0-1 days;

(3) Repeatedly add antimonate to the solution once, control the molar ratio of sulfate/antimonate (1-5):1, the mass ratio of organic carbon/antimony (30-80):1, and the reaction time is 1 days, and then obtain highly efficient ecological restoration flora.

The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art can still understand the foregoing embodiments. Modifications are made to the technical solutions described, or equivalent replacements are made to some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions claimed in the present invention.

Claims (4)

1. A method utilizing biogas residues to prepare efficient ecological restoration flora of Sb polluted sites is characterized in that, by adding carbon source and sulfate in biogas residues, sulfate-reducing flora in biogas residues is grown rapidly, and sulfide is produced simultaneously Subsequent addition of antimonate consumes the sulfide that inhibits the growth of sulfate-reducing bacteria, and the presence of sulfide also greatly reduces the toxicity of antimonate to sulfate-reducing bacteria, and at the same time makes the cultured sulfate-reducing bacteria resistant to Sb performance improvement, which includes the following steps: (1) Mix biogas residue with a solution containing a certain amount of carbon source, add a certain amount of sulfate, and put it into a reactor for biochemical reaction. Control the content of dry biogas residue in the mixed solution to 0.1-20%, and the solid content to 0.5- 25%, the organic carbon content is 0.5-20%, the mass ratio of organic carbon/sulfate (calculated as SO4) is (1-50):1, and then reacted for 0.1-5 days; (2) in the solution after step (1) reaction, add a certain amount of antimonate, control the mol ratio (1-50) of sulfate/antimonate: 1, the mass ratio of organic carbon/antimony (10- 100): 1, followed by 0-5 days of reaction; (3) Repeatedly add antimonate to the solution 0-4 times, each time controlling the molar ratio of sulfate/antimonate (1-50):1, the mass ratio of organic carbon/antimony (10-100): 1. The reaction time is 0-5 days, and then high-efficiency ecological restoration flora can be obtained. 2. a kind of method utilizing biogas residue to prepare Sb polluted site efficient ecological restoration flora as claimed in claim 1, is characterized in that, described biogas residue is agriculture, food industry organic waste carries out dry type, semi-dry type and Solid residues from wet anaerobic fermentation processes. 3. a kind of method utilizing biogas residues to prepare efficient ecological restoration flora of Sb polluted site as claimed in claim 1, is characterized in that, described carbon source solution can be that contains the organic matters such as alcohol, carbohydrate, protein, starch The solution may also be a solution containing organic solid waste such as industrial organic waste, kitchen waste, or industrial and municipal organic waste water. 4. a kind of method utilizing biogas residue to prepare efficient ecological restoration flora of Sb polluted site as claimed in claim 1, is characterized in that, described sulfate-containing salt is sodium sulfate, potassium sulfate, ferrous sulfate, ferric sulfate etc. Salts containing sulfate; the antimonate is a salt containing antimonate.