CN105836899A - Method for environment-friendly efficient restoration against marine oil spill - Google Patents
Method for environment-friendly efficient restoration against marine oil spill Download PDFInfo
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- CN105836899A CN105836899A CN201610370629.4A CN201610370629A CN105836899A CN 105836899 A CN105836899 A CN 105836899A CN 201610370629 A CN201610370629 A CN 201610370629A CN 105836899 A CN105836899 A CN 105836899A
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- 238000000034 method Methods 0.000 title claims abstract description 43
- 239000003305 oil spill Substances 0.000 title claims abstract description 28
- 238000000855 fermentation Methods 0.000 claims abstract description 59
- 230000004151 fermentation Effects 0.000 claims abstract description 59
- 230000015556 catabolic process Effects 0.000 claims abstract description 49
- 238000006731 degradation reaction Methods 0.000 claims abstract description 49
- 239000007788 liquid Substances 0.000 claims abstract description 49
- 239000000463 material Substances 0.000 claims abstract description 40
- 239000000203 mixture Substances 0.000 claims abstract description 29
- 229950008882 polysorbate Drugs 0.000 claims abstract description 21
- 229920000136 polysorbate Polymers 0.000 claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- 235000021307 Triticum Nutrition 0.000 claims abstract description 16
- 235000013339 cereals Nutrition 0.000 claims abstract description 16
- 238000002156 mixing Methods 0.000 claims abstract description 16
- 239000000843 powder Substances 0.000 claims abstract description 16
- 239000010902 straw Substances 0.000 claims abstract description 16
- 239000002023 wood Substances 0.000 claims abstract description 16
- 239000010779 crude oil Substances 0.000 claims abstract description 15
- 239000013535 sea water Substances 0.000 claims abstract description 14
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 13
- 238000001914 filtration Methods 0.000 claims abstract description 11
- 244000294611 Punica granatum Species 0.000 claims abstract description 4
- 235000014360 Punica granatum Nutrition 0.000 claims abstract description 4
- 239000003921 oil Substances 0.000 claims description 102
- 241000894006 Bacteria Species 0.000 claims description 39
- 125000000217 alkyl group Chemical group 0.000 claims description 28
- 239000011521 glass Substances 0.000 claims description 24
- 239000002689 soil Substances 0.000 claims description 17
- 238000005303 weighing Methods 0.000 claims description 16
- 241000251468 Actinopterygii Species 0.000 claims description 15
- 241001506047 Tremella Species 0.000 claims description 15
- 241000209140 Triticum Species 0.000 claims description 15
- 235000009392 Vitis Nutrition 0.000 claims description 15
- 241000219095 Vitis Species 0.000 claims description 15
- 239000012634 fragment Substances 0.000 claims description 15
- 210000000582 semen Anatomy 0.000 claims description 15
- 239000008213 purified water Substances 0.000 claims description 13
- 238000003756 stirring Methods 0.000 claims description 8
- 239000004575 stone Substances 0.000 claims description 5
- 230000000844 anti-bacterial effect Effects 0.000 claims description 4
- 239000000835 fiber Substances 0.000 claims 1
- -1 alkyl glycoside Chemical class 0.000 abstract description 2
- 230000008901 benefit Effects 0.000 abstract description 2
- 229930182470 glycoside Natural products 0.000 abstract description 2
- 241000283690 Bos taurus Species 0.000 abstract 1
- 244000207740 Lemna minor Species 0.000 abstract 1
- 235000006439 Lemna minor Nutrition 0.000 abstract 1
- 235000009811 Momordica charantia Nutrition 0.000 abstract 1
- 240000000249 Morus alba Species 0.000 abstract 1
- 235000008708 Morus alba Nutrition 0.000 abstract 1
- 235000001855 Portulaca oleracea Nutrition 0.000 abstract 1
- 241000908178 Tremella fuciformis Species 0.000 abstract 1
- 244000078912 Trichosanthes cucumerina Species 0.000 abstract 1
- 235000008322 Trichosanthes cucumerina Nutrition 0.000 abstract 1
- 244000098338 Triticum aestivum Species 0.000 abstract 1
- 235000009754 Vitis X bourquina Nutrition 0.000 abstract 1
- 235000012333 Vitis X labruscana Nutrition 0.000 abstract 1
- 240000006365 Vitis vinifera Species 0.000 abstract 1
- 235000014787 Vitis vinifera Nutrition 0.000 abstract 1
- 230000000593 degrading effect Effects 0.000 abstract 1
- 239000003208 petroleum Substances 0.000 abstract 1
- 229910052902 vermiculite Inorganic materials 0.000 abstract 1
- 235000019354 vermiculite Nutrition 0.000 abstract 1
- 239000010455 vermiculite Substances 0.000 abstract 1
- 230000007613 environmental effect Effects 0.000 description 13
- 230000000694 effects Effects 0.000 description 9
- 231100000252 nontoxic Toxicity 0.000 description 6
- 230000003000 nontoxic effect Effects 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 238000012360 testing method Methods 0.000 description 5
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- 238000006065 biodegradation reaction Methods 0.000 description 3
- 239000003599 detergent Substances 0.000 description 3
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- 241000186361 Actinobacteria <class> Species 0.000 description 2
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- 241000186063 Arthrobacter Species 0.000 description 1
- 241000186074 Arthrobacter globiformis Species 0.000 description 1
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- 238000010521 absorption reaction Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
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- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
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- 150000008131 glucosides Chemical class 0.000 description 1
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- 238000011084 recovery Methods 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/344—Biological treatment of water, waste water, or sewage characterised by the microorganisms used for digestion of mineral oil
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/08—Seawater, e.g. for desalination
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- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Organic Chemistry (AREA)
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Abstract
The invention relates to a method for environment-friendly efficient restoration against marine oil spill. The method comprises the following steps: a step (1) of taking, by mass parts, 20-27 parts of bran, 20-27 parts of wheat straw, 19-25 parts of wood shavings, 16-22 parts of duckweed, 12-17 parts of grape seeds, 10-17 parts of apple peel, 9-16 parts of pomegranate rind, 9-16 parts of scale, 7-14 parts of bitter gourd seedlings, 7-14 parts of mulberry leaves, 5-9 parts of tremella fuciformis, 3-4 parts of vermiculite, 6-10 parts of east wall earth, 4-5 parts of distillers' dried grains and 3-5 parts of dry yeast powder; a step (2) of adding purifier water into the mixed material in the step (1), performing fermentation in the absence of light, filtering the mixed materials, and taking obtained fermentation liquid; a step (3) of evenly mixing the fermentation liquid, alkyl glycoside and polysorbate; a step (4) of placing petroleum degrading strains and the mixture obtained in the step (3) into seawater polluted by crude oil. The method has the advantage of being high in oil spill degradation efficiency.
Description
Technical field
The invention belongs to marine oil spill and pollute process field, particularly relate to the environmental protection life that a kind of marine oil spill pollutes
State restorative procedure.
Background technology
Along with the whole world is to oil product demand and the increase of oil sea transport, oil transportation at sea and marine stone
Oil yield increases sharply, and marine oil overflow accident is also on the increase, and ocean and coastal land-based area in serious threat
Ecological environment.In recent years, the most serious marine oil spill pollute cause countries in the world marine management department and
The concern of ecologist.Oil spilling mostly occurs at sea, not only causes along with stormy waves drifts to the oil product in coastline
Heavy environmental pollution, and owing to genotoxic potential and the biological accumulation effect of petroleum hydrocarbon class pollutant can cause closely
Bank marine environment quality and biological species diversity indices degradation, marine ecosystems subject to severe risks of damage,
Also aquatic products industry and tourist industry can be brought about great losses.
At present, the main method processing marine oil spill pollution has Physical, chemical method, bioanalysis and burning method.
Physical is mainly included in the oil spilling waters laying oil fence and prevents oil spilling from spreading, and organizes shipping work of removing contamination to adopt
Take some physical methods such as adsorbing material absorption such as machinery recovery, asphalt felt such as oil skimmer by Treatment.Cloth
If the oil fence can also process in conjunction with burning method.Method of chemical treatment be mainly in water spray detergent,
The collection chemical agent such as oil preparation and firming agent, processes after oil spilling dispersion or solidification again.Biological restoration
Degradation mainly by microorganism removes oil spilling, including adding nutrient substance and adding strain two kinds
Mode.But, these Treatment methods all have some limitations.Use traditional Physical efficiency
Relatively low, labor intensity is big, and is difficult to Treatment is thorough.Ocean is held by most detergents and collection oil preparation
It is easily generated secondary pollution.The advantage using bioanalysis reparation to repair crude oil pollution sea water is safety non-toxic, can give birth to
Thing is degraded, and environment does not the most bring secondary pollution, but deposits when using microorganism remediation crude oil pollution sea water
In problems with: degradation efficiency is low, utilization rate is low, it is not suitable for large area marine oil spill reparation.
Summary of the invention
It is an object of the invention to overcome the deficiencies in the prior art, it is provided that it is environmental and ecological that a kind of marine oil spill pollutes
Restorative procedure.
In order to solve above-mentioned technical problem, the present invention by the following technical solutions: a kind of marine oil spill pollute
Environmental and ecological restorative procedure, comprises the following steps:
(1) each component of following mass fraction is weighed according to following proportioning: Testa oryzae 20-27 part, wheat straw 20-27
Part, wood fragments bits 19-25 part, Herba Spirodelae 16-22 part, Semen Vitis viniferae 12-17 part, Pericarpium Mali pumilae 10-17 part, stone
Pomegranate skin 9-16 part, fish scale 9-16 part, Fructus Momordicae charantiae seedling 7-14 part, Folium Mori 7-14 part, Tremella 5-9 part, trematodiasis
Stone 3-4 part, east wall soil 6-10 part, distiller's dried grain 4-5 part, dried yeast powder 3-5 part, by above-mentioned load weighted
Each component is pulverized respectively and crosses 80 mesh sieves；
(2) by each component mix homogeneously in step (1), obtain mixed material, add in mixed material
Weight is the purified water of 0.9-1.2 times of mixed material, and stirring and evenly mixing puts into glass container, seal glass
Container, puts into glass container lucifuge in the water-bath of 25-30 DEG C and ferments 42-50 hour, obtain tunning,
By gained tunning 100 eye mesh screens, take the fermentation liquid filtering gained；
(3) weighing quality is fermentation liquid quality 0.9～the alkyl polyglucoside of 1.1 times, and weighing quality is fermentation liquid
Quality 0.6～the Polysorbate of 0.9 times, mix load weighted alkyl polyglucoside, Polysorbate all with fermentation liquid
Even；
(4) in the sea water of crude oil pollution, throw in oil degradation bacteria and the mixture of step (3) gained, throw
The quality of the oil degradation bacteria put is the 4%～6% of oil spillage, the matter of the mixture of step (3) gained of input
Amount is the 15%～20% of oil spillage, makes oil spilling region keep turbulent fluctuation state by turbine type agitator.
In the present invention, Polysorbate is faint yellow to orange-yellow thick liquid, is nonionic surfactant,
Nantural non-toxic, can be as food emulsifying agent；Alkyl polyglucoside is nonionic surfactant, and alkyl polyglucoside is white
Color solid, alkyl polyglucoside not only environment-protecting asepsis is non-stimulated, and biodegradation is thorough rapidly, cheap,
Surface tension is low, and soil release performance is good；Testa oryzae, wheat straw, wood fragments bits, Herba Spirodelae, Semen Vitis viniferae, Pericarpium Mali pumilae, Punica granatum L.
Skin, fish scale, Fructus Momordicae charantiae seedling, Folium Mori, Tremella, Vermiculitum and east wall soil are with low cost, itself and distiller's dried grain and dry ferment
The product of female powder 42-50 hour gained of fermentation itself does not have a significant oil degradation effect, but fermentation liquid with
Alkyl polyglucoside, poly-Pyrusussuriensis and oil degradation bacteria are thrown to oil spilling marine site after mixing with said ratio can be to oil
Produce good degradation effect, substantially increase Treatment efficiency, the fermentation liquid of step (2) gained is described
And alkyl polyglucoside, between poly-Pyrusussuriensis and oil degradation bacteria, can there is synergism.Repetition test shows,
In step (2), in mixed material add weight be mixed material 0.9-1.2 times purified water and mixing can
To improve fermentation efficiency, shorten fermentation time (only needing 42-50 hour to complete fermentation), improve mixing
The utilization rate of material.Additionally, the equal environmental protection of each component of the present invention is nontoxic, environment will not be brought secondary
Polluting, biodegradability is good, wherein, in step (2), " by gained tunning 100 eye mesh screens,
Take the fermentation liquid filtering gained " purpose be that the residue reduced in tunning enters oil spilling marine site, it is to avoid right
Environment brings secondary pollution.
Further, described oil degradation bacteria is spherical node antibacterial, and cell concentration is 109～1010cfu/g.Institute
The spherical node antibacterial Classification system Arthrobacter globiformis stated, belongs to antibacterial circle Bacteria,
Actinomycetes door Actinobacteria, Actinomycetes Actinobacteria, Actinomycetal
Actinomycetales, micrococcaceae Micrococcaceae, Arthrobacter Arthrobacter.
Preferably, the mass fraction of each component weighed in step (1) is as follows: 25 parts of Testa oryzae, wheat straw 25
Part, 22 parts of wood fragments bits, 18 parts of Herba Spirodelae, Semen Vitis viniferae 15 parts, Pericarpium Mali pumilae 14 parts, Pericarpium Granati 12 parts, fish
12 parts of squama, Fructus Momordicae charantiae seedling 10 parts, 10 parts of Folium Mori, 7 parts of Tremella, Vermiculitum 3 parts, east wall soil 8 parts, dry wine
Grain 5 parts, dried yeast powder 4 parts.
Preferably, the mass fraction of each component weighed in step (1) is as follows: 24 parts of Testa oryzae, wheat straw 24
Part, 23 parts of wood fragments bits, 19 parts of Herba Spirodelae, Semen Vitis viniferae 15 parts, Pericarpium Mali pumilae 13 parts, Pericarpium Granati 13 parts, fish
13 parts of squama, Fructus Momordicae charantiae seedling 11 parts, 11 parts of Folium Mori, 8 parts of Tremella, Vermiculitum 4 parts, east wall soil 8 parts, dry wine
Grain 4 parts, dried yeast powder 4 parts.
Preferably, in step (2), purified water weight is mixed material 1.1 added in mixed material
Times.
Preferably, in step (3), alkyl polyglucoside quality is fermentation liquid quality 1.0 times weighed.
Preferably, in step (3), Polysorbate quality is fermentation liquid quality 0.8 times weighed.
Preferably, in step (4), the quality of the oil degradation bacteria thrown in the sea water of crude oil pollution is excessive
The 5% of oil mass, quality is oil spillage the 16% of the mixture of step (3) gained of input.
Further, in step (4), throw in oil spilling district before oil degradation bacteria, first oil degradation bacteria is filled
Enter in gunnysack.Having fold on the inner surface of described gunnysack, the density of the fold at gunnysack limit and angle is more than gunnysack
The density of its elsewhere fold.The inner surface of gunnysack arranges fold and can improve the performance of gunnysack water suction water storage,
When preventing ebb tide, degradation bacteria is dead because being excessively dried, and fold is set on the inner surface of gunnysack can be effective
The oil degradation bacteria contained in preventing gunnysack is washed away by current.
Compared with prior art, the invention has the beneficial effects as follows: in the present invention, Polysorbate be faint yellow extremely
Orange-yellow thick liquid, is nonionic surfactant, and Nantural non-toxic can be as food emulsifying agent；Alkane
Base glucosides is nonionic surfactant, and alkyl polyglucoside is white solid, alkyl polyglucoside not only environment-protecting asepsis without
Stimulating, and biodegradation is thorough rapidly, cheap, surface tension is low, and soil release performance is good；Testa oryzae, wheat
Straw, wood fragments bits, Herba Spirodelae, Semen Vitis viniferae, Pericarpium Mali pumilae, Pericarpium Granati, fish scale, Fructus Momordicae charantiae seedling, Folium Mori, Tremella,
Vermiculitum and east wall soil are with low cost, and the product of itself and distiller's dried grain and dried yeast powder 42-50 hour gained of fermentation is originally
Body does not have a significant oil degradation effect, but fermentation liquid and alkyl polyglucoside, poly-Pyrusussuriensis and oil degradation bacteria
It is thrown to oil spilling marine site after mixing with said ratio and oil can be produced good degradation effect, substantially increase
Treatment efficiency, illustrates that the fermentation liquid of step (2) gained can be with alkyl polyglucoside, poly-Pyrusussuriensis and oil
There is between degradation bacteria synergism.Repetition test shows, in step (2), adds weight in mixed material
Purified water mixing that amount is mixed material 0.9-1.2 times can improve fermentation efficiency, (only shorten fermentation time
Need 42-50 hour to complete fermentation), improve the utilization rate of mixed material.Additionally, each group of the present invention
Dividing equal environmental protection nontoxic, environment will not bring secondary pollution, biodegradability is good, wherein, and step
(2), in, the purpose of " by gained tunning 100 eye mesh screens, take the fermentation liquid filtering gained " is to subtract
Residue in few tunning enters oil spilling marine site, it is to avoid environment is brought secondary pollution.
Detailed description of the invention
Below example facilitates a better understanding of the present invention, but does not limit the present invention.In following embodiment
Experimental technique, if no special instructions, be conventional method.
Embodiment 1
The environmental and ecological restorative procedure that a kind of marine oil spill pollutes, comprises the following steps:
(1) weigh each component of following mass fraction according to following proportioning: 21 parts of Testa oryzae, wheat straw 21 parts,
20 parts of wood fragments bits, 16 parts of Herba Spirodelae, Semen Vitis viniferae 14 parts, Pericarpium Mali pumilae 10 parts, Pericarpium Granati 9 parts, fish scale 9
Part, Fructus Momordicae charantiae seedling 7 parts, 9 parts of Folium Mori, 5 parts of Tremella, Vermiculitum 3 parts, east wall soil 6 parts, distiller's dried grain 4 parts,
Dried yeast powder 3 parts, pulverizes above-mentioned load weighted each component respectively and crosses 80 mesh sieves；
(2) by each component mix homogeneously in step (1), obtain mixed material, add in mixed material
Weight is the purified water of 1.2 times of mixed material, stirring and evenly mixing, puts into glass container, sealed glass container,
Glass container is put into lucifuge in the water-bath of 25-30 DEG C ferment 45 hours, obtain tunning, gained is sent out
Ferment product 100 eye mesh screens, take the fermentation liquid filtering gained；
(3) weighing the alkyl polyglucoside that quality is fermentation liquid quality 1.0 times, weighing quality is fermentation liquid quality 0.9
Polysorbate again, mixs homogeneously load weighted alkyl polyglucoside, Polysorbate with fermentation liquid；
(4) in the sea water of crude oil pollution, throw in oil degradation bacteria and the mixture of step (3) gained, throw
The quality of the oil degradation bacteria put is the 4% of oil spillage, and the quality of the mixture of step (3) gained of input is
The 15% of oil spillage, makes oil spilling region keep turbulent fluctuation state by turbine type agitator.
Embodiment 2
The environmental and ecological restorative procedure that a kind of marine oil spill pollutes, comprises the following steps:
(1) weigh each component of following mass fraction according to following proportioning: 20 parts of Testa oryzae, wheat straw 20 parts,
19 parts of wood fragments bits, 19 parts of Herba Spirodelae, Semen Vitis viniferae 12 parts, Pericarpium Mali pumilae 10 parts, Pericarpium Granati 10 parts, fish scale 9
Part, Fructus Momordicae charantiae seedling 9 parts, 7 parts of Folium Mori, 6 parts of Tremella, Vermiculitum 4 parts, east wall soil 6 parts, distiller's dried grain 4 parts,
Dried yeast powder 4 parts, pulverizes above-mentioned load weighted each component respectively and crosses 80 mesh sieves；
(2) by each component mix homogeneously in step (1), obtain mixed material, add in mixed material
Weight is the purified water of 0.9 times of mixed material, stirring and evenly mixing, puts into glass container, sealed glass container,
Glass container is put into lucifuge in the water-bath of 25-30 DEG C ferment 44 hours, obtain tunning, gained is sent out
Ferment product 100 eye mesh screens, take the fermentation liquid filtering gained；
(3) weighing the alkyl polyglucoside that quality is fermentation liquid quality 1.1 times, weighing quality is fermentation liquid quality 0.6
Polysorbate again, mixs homogeneously load weighted alkyl polyglucoside, Polysorbate with fermentation liquid；
(4) in the sea water of crude oil pollution, throw in oil degradation bacteria and the mixture of step (3) gained, throw
The quality of the oil degradation bacteria put is the 4% of oil spillage, and the quality of the mixture of step (3) gained of input is
The 20% of oil spillage, makes oil spilling region keep turbulent fluctuation state by turbine type agitator.
Embodiment 3
The environmental and ecological restorative procedure that a kind of marine oil spill pollutes, comprises the following steps:
(1) weigh each component of following mass fraction according to following proportioning: 25 parts of Testa oryzae, wheat straw 25 parts,
24 parts of wood fragments bits, 22 parts of Herba Spirodelae, Semen Vitis viniferae 17 parts, Pericarpium Mali pumilae 17 parts, Pericarpium Granati 16 parts, fish scale 16
Part, Fructus Momordicae charantiae seedling 14 parts, 14 parts of Folium Mori, 8 parts of Tremella, Vermiculitum 4 parts, east wall soil 8 parts, distiller's dried grain 5
Part, dried yeast powder 5 parts, pulverize above-mentioned load weighted each component respectively and cross 80 mesh sieves；
(2) by each component mix homogeneously in step (1), obtain mixed material, add in mixed material
Weight is the purified water of 1.0 times of mixed material, stirring and evenly mixing, puts into glass container, sealed glass container,
Glass container is put into lucifuge in the water-bath of 25-30 DEG C ferment 46 hours, obtain tunning, gained is sent out
Ferment product 100 eye mesh screens, take the fermentation liquid filtering gained；
(3) weighing the alkyl polyglucoside that quality is fermentation liquid quality 1.1 times, weighing quality is fermentation liquid quality 0.8
Polysorbate again, mixs homogeneously load weighted alkyl polyglucoside, Polysorbate with fermentation liquid；
(4) in the sea water of crude oil pollution, throw in oil degradation bacteria and the mixture of step (3) gained, throw
The quality of the oil degradation bacteria put is the 6% of oil spillage, and the quality of the mixture of step (3) gained of input is
The 18% of oil spillage, makes oil spilling region keep turbulent fluctuation state by turbine type agitator.
Embodiment 4
The environmental and ecological restorative procedure that a kind of marine oil spill pollutes, comprises the following steps:
(1) weigh each component of following mass fraction according to following proportioning: 27 parts of Testa oryzae, wheat straw 27 parts,
25 parts of wood fragments bits, 21 parts of Herba Spirodelae, Semen Vitis viniferae 16 parts, Pericarpium Mali pumilae 16 parts, Pericarpium Granati 15 parts, fish scale 15
Part, Fructus Momordicae charantiae seedling 14 parts, 14 parts of Folium Mori, 9 parts of Tremella, Vermiculitum 3 parts, east wall soil 10 parts, distiller's dried grain 5
Part, dried yeast powder 5 parts, pulverize above-mentioned load weighted each component respectively and cross 80 mesh sieves；
(2) by each component mix homogeneously in step (1), obtain mixed material, add in mixed material
Weight is the purified water of 1.2 times of mixed material, stirring and evenly mixing, puts into glass container, sealed glass container,
Glass container is put into lucifuge in the water-bath of 25-30 DEG C ferment 50 hours, obtain tunning, gained is sent out
Ferment product 100 eye mesh screens, take the fermentation liquid filtering gained；
(3) weighing the alkyl polyglucoside that quality is fermentation liquid quality 0.9 times, weighing quality is fermentation liquid quality 0.9
Polysorbate again, mixs homogeneously load weighted alkyl polyglucoside, Polysorbate with fermentation liquid；
(4) in the sea water of crude oil pollution, oil degradation bacteria and the mixture of step (3) gained are thrown in, to
Before oil degradation bacteria is thrown in oil spilling district, first oil degradation bacteria is loaded in gunnysack；On the inner surface of described gunnysack
Having fold, the density of the fold at gunnysack limit and angle is more than the density of its elsewhere fold of gunnysack；The oil thrown in
The quality of degradation bacteria is the 6% of oil spillage, and the quality of the mixture of step (3) gained of input is oil spillage
20%, make oil spilling region keep turbulent fluctuation state by turbine type agitator.
Embodiment 5
The environmental and ecological restorative procedure that a kind of marine oil spill pollutes, comprises the following steps:
(1) weigh each component of following mass fraction according to following proportioning: 25 parts of Testa oryzae, wheat straw 25 parts,
22 parts of wood fragments bits, 18 parts of Herba Spirodelae, Semen Vitis viniferae 15 parts, Pericarpium Mali pumilae 14 parts, Pericarpium Granati 12 parts, fish scale 12
Part, Fructus Momordicae charantiae seedling 10 parts, 10 parts of Folium Mori, 7 parts of Tremella, Vermiculitum 3 parts, east wall soil 8 parts, distiller's dried grain 5
Part, dried yeast powder 4 parts, pulverize above-mentioned load weighted each component respectively and cross 80 mesh sieves；
(2) by each component mix homogeneously in step (1), obtain mixed material, add in mixed material
Weight is the purified water of 1.0 times of mixed material, stirring and evenly mixing, puts into glass container, sealed glass container,
Glass container is put into lucifuge in the water-bath of 25-30 DEG C ferment 44 hours, obtain tunning, gained is sent out
Ferment product 100 eye mesh screens, take the fermentation liquid filtering gained；
(3) weighing the alkyl polyglucoside that quality is fermentation liquid quality 1.0 times, weighing quality is fermentation liquid quality 0.8
Polysorbate again, mixs homogeneously load weighted alkyl polyglucoside, Polysorbate with fermentation liquid；
(4) in the sea water of crude oil pollution, oil degradation bacteria and the mixture of step (3) gained are thrown in, to
Before oil degradation bacteria is thrown in oil spilling district, first oil degradation bacteria is loaded in gunnysack；On the inner surface of described gunnysack
Having fold, the density of the fold at gunnysack limit and angle is more than the density of its elsewhere fold of gunnysack；The oil thrown in
The quality of degradation bacteria is the 5% of oil spillage, and the quality of the mixture of step (3) gained of input is oil spillage
18%, make oil spilling region keep turbulent fluctuation state by turbine type agitator.
Embodiment 6
The environmental and ecological restorative procedure that a kind of marine oil spill pollutes, comprises the following steps:
(1) weigh each component of following mass fraction according to following proportioning: 24 parts of Testa oryzae, wheat straw 24 parts,
23 parts of wood fragments bits, 19 parts of Herba Spirodelae, Semen Vitis viniferae 15 parts, Pericarpium Mali pumilae 13 parts, Pericarpium Granati 13 parts, fish scale 13
Part, Fructus Momordicae charantiae seedling 11 parts, 11 parts of Folium Mori, 8 parts of Tremella, Vermiculitum 4 parts, east wall soil 8 parts, distiller's dried grain 4
Part, dried yeast powder 4 parts, pulverize above-mentioned load weighted each component respectively and cross 80 mesh sieves；
(2) by each component mix homogeneously in step (1), obtain mixed material, add in mixed material
Weight is the purified water of 1.1 times of mixed material, stirring and evenly mixing, puts into glass container, sealed glass container,
Glass container is put into lucifuge in the water-bath of 25-30 DEG C ferment 42 hours, obtain tunning, gained is sent out
Ferment product 100 eye mesh screens, take the fermentation liquid filtering gained；
(3) weighing the alkyl polyglucoside that quality is fermentation liquid quality 1.0 times, weighing quality is fermentation liquid quality 0.8
Polysorbate again, mixs homogeneously load weighted alkyl polyglucoside, Polysorbate with fermentation liquid；
(4) in the sea water of crude oil pollution, oil degradation bacteria and the mixture of step (3) gained are thrown in, to
Before oil degradation bacteria is thrown in oil spilling district, first oil degradation bacteria is loaded in gunnysack；On the inner surface of described gunnysack
Having fold, the density of the fold at gunnysack limit and angle is more than the density of its elsewhere fold of gunnysack；The oil thrown in
The quality of degradation bacteria is the 5% of oil spillage, and the quality of the mixture of step (3) gained of input is oil spillage
16%, make oil spilling region keep turbulent fluctuation state by turbine type agitator.
Experimental result
Choose 18 identical ponds of size specification (pond specification is 7m × 3m × 1.5m), and it is numbered
For No. 1-18, injecting 25L sea water, and add 500g crude oil in each pond, oil selects Shengli crude,
The basic physical property of selected crude oil is as follows: density 0.8695g/cm3(testing standard
GB/T1884-2000), 33 DEG C, rate of shear 20 time viscosity 230mPa.s.1st, 7, No. 13 ponds are by this
The method of inventive embodiments 1 processes, at the 2nd, 8, No. 14 methods by the embodiment of the present invention 2
Reason, the 3rd, 9, No. 15 are processed by the method for the embodiment of the present invention 3, press this for the 4th, 10, No. 16
The method of bright embodiment 4 processes, at the 5th, 11, No. 17 methods by the embodiment of the present invention 5
Reason, the 6th, 12, No. 18 are processed by the method for the embodiment of the present invention 6, throw respectively in each process group
After putting the detergent 12h of its step (4) gained, measure the weight of unrecovered oil respectively, according to being initially added
Oil mass conversion obtains Treatment effect, measurement result such as table 1:
The Treatment effect of table 1 different disposal group
Test result indicate that, oil degradation is imitated by the environmental and ecological restorative procedure that the marine oil spill of the present invention pollutes
Rate is high.In the present invention, Testa oryzae, wheat straw, wood fragments bits, Herba Spirodelae, Semen Vitis viniferae, Pericarpium Mali pumilae, Pericarpium Granati, fish
Squama, Fructus Momordicae charantiae seedling, Folium Mori, Tremella, Vermiculitum and east wall soil are with low cost, and it is sent out with distiller's dried grain and dried yeast powder
The product of 42-50 hour gained of ferment itself does not have significant oil degradation effect, but fermentation liquid is sugared with alkyl
Glycosides, poly-Pyrusussuriensis and oil degradation bacteria are thrown to oil spilling marine site and can produce good to oil after mixing with said ratio
Good degradation effect, substantially increases Treatment efficiency, illustrates that the fermentation liquid of step (2) gained can be with
Alkyl polyglucoside, between poly-Pyrusussuriensis and oil degradation bacteria, there is synergism.Repetition test shows, step (2)
In, in mixed material add weight be mixed material 0.9-1.2 times purified water and mixing can improve fermentation
Efficiency, shortens fermentation time (only needing 42-50 hour to complete fermentation), improves the utilization rate of mixed material.
Additionally, the equal environmental protection of each component of the present invention is nontoxic, environment will not be brought secondary pollution, biodegradation
Performance is good, wherein, in step (2), " by gained tunning 100 eye mesh screens, takes and filters gained
Fermentation liquid " purpose be that the residue reduced in tunning enters oil spilling marine site, it is to avoid environment is brought secondary
Pollute.
The above is only the preferred embodiment of the present invention, it should be pointed out that: common for the art
For technical staff, under the premise without departing from the principles of the invention, it is also possible to make some improvement, these change
Enter to should be regarded as protection scope of the present invention.
Claims (10)
1. the environment-friendly high-efficiency restorative procedure that a marine oil spill pollutes, it is characterised in that: comprise the following steps:
(1) each component of following mass fraction is weighed according to following proportioning: Testa oryzae 20-27 part, wheat straw 20-27
Part, wood fragments bits 19-25 part, Herba Spirodelae 16-22 part, Semen Vitis viniferae 12-17 part, Pericarpium Mali pumilae 10-17 part, stone
Pomegranate skin 9-16 part, fish scale 9-16 part, Fructus Momordicae charantiae seedling 7-14 part, Folium Mori 7-14 part, Tremella 5-9 part, trematodiasis
Stone 3-4 part, east wall soil 6-10 part, distiller's dried grain 4-5 part, dried yeast powder 3-5 part, by above-mentioned load weighted
Each component is pulverized respectively and crosses 80 mesh sieves；
(2) by each component mix homogeneously in step (1), obtain mixed material, add in mixed material
Weight is the purified water of 0.9-1.2 times of mixed material, and stirring and evenly mixing puts into glass container, seal glass
Container, puts into glass container lucifuge in the water-bath of 25-30 DEG C and ferments 42-50 hour, obtain tunning,
By gained tunning 100 eye mesh screens, take the fermentation liquid filtering gained；
(3) weighing quality is fermentation liquid quality 0.9～the alkyl polyglucoside of 1.1 times, and weighing quality is fermentation liquid
Quality 0.6～the Polysorbate of 0.9 times, mix load weighted alkyl polyglucoside, Polysorbate all with fermentation liquid
Even；
(4) in the sea water of crude oil pollution, throw in oil degradation bacteria and the mixture of step (3) gained, throw
The quality of the oil degradation bacteria put is the 4%～6% of oil spillage, the matter of the mixture of step (3) gained of input
Amount is the 15%～20% of oil spillage, makes oil spilling region keep turbulent fluctuation state by turbine type agitator.
The environment-friendly high-efficiency restorative procedure that marine oil spill the most according to claim 1 pollutes, it is characterised in that:
The mass fraction of each component weighed in step (1) is as follows: 25 parts of Testa oryzae, wheat straw 25 parts, wood fragments bits 22
Part, 18 parts of Herba Spirodelae, Semen Vitis viniferae 15 parts, Pericarpium Mali pumilae 14 parts, Pericarpium Granati 12 parts, 12 parts of fish scale, Fructus Momordicae charantiae
Seedling 10 parts, 10 parts of Folium Mori, 7 parts of Tremella, Vermiculitum 3 parts, 8 parts of east wall soil, distiller's dried grain 5 parts, dry ferment
Female 4 parts of powder.
The environment-friendly high-efficiency restorative procedure that marine oil spill the most according to claim 1 pollutes, it is characterised in that:
The mass fraction of each component weighed in step (1) is as follows: 24 parts of Testa oryzae, wheat straw 24 parts, wood fragments bits 23
Part, 19 parts of Herba Spirodelae, Semen Vitis viniferae 15 parts, Pericarpium Mali pumilae 13 parts, Pericarpium Granati 13 parts, 13 parts of fish scale, Fructus Momordicae charantiae
Seedling 11 parts, 11 parts of Folium Mori, 8 parts of Tremella, Vermiculitum 4 parts, 8 parts of east wall soil, distiller's dried grain 4 parts, dry ferment
Female 4 parts of powder.
The environment-friendly high-efficiency restorative procedure that marine oil spill the most according to claim 1 pollutes, it is characterised in that:
In step (2), purified water weight is mixed material 1.1 times added in mixed material.
The environment-friendly high-efficiency restorative procedure that marine oil spill the most according to claim 1 pollutes, it is characterised in that:
In step (3), alkyl polyglucoside quality is fermentation liquid quality 1.0 times weighed.
The environment-friendly high-efficiency restorative procedure that marine oil spill the most according to claim 1 pollutes, it is characterised in that:
In step (3), Polysorbate quality is fermentation liquid quality 0.8 times weighed.
The environment-friendly high-efficiency restorative procedure that marine oil spill the most according to claim 1 pollutes, it is characterised in that:
In step (4), quality is oil spillage the 5% of the oil degradation bacteria thrown in the sea water of crude oil pollution, throw
Quality is oil spillage the 16% of the mixture of step (3) gained put.
The environment-friendly high-efficiency restorative procedure that marine oil spill the most according to claim 1 pollutes, it is characterised in that:
Described oil degradation bacteria is spherical node antibacterial, and cell concentration is 109～1010cfu/g。
9. the environment-friendly high-efficiency restorative procedure polluted according to the marine oil spill according to any one of claim 1-8, its
It is characterised by: in step (4), throws in oil spilling district before oil degradation bacteria, first oil degradation bacteria is loaded fiber crops
In Dai.
The environment-friendly high-efficiency restorative procedure that marine oil spill the most according to claim 1 pollutes, it is characterised in that:
Having fold on the inner surface of described gunnysack, the density of the fold at gunnysack limit and angle is more than its elsewhere fold of gunnysack
Density.
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