CN105816853A - Modified antibacterial peptide and preparation of gel - Google Patents
Modified antibacterial peptide and preparation of gel Download PDFInfo
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- CN105816853A CN105816853A CN201510001466.8A CN201510001466A CN105816853A CN 105816853 A CN105816853 A CN 105816853A CN 201510001466 A CN201510001466 A CN 201510001466A CN 105816853 A CN105816853 A CN 105816853A
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Abstract
The invention belongs to the field of preparation of gel and specifically relates to modified antibacterial peptide HC-17 gel of Hydrophis cyanocinctus-derived antibacterial peptide Hc-CATH and a preparation method of the modified antibacterial peptide HC-17 gel. The preparation method comprises the following specific steps: (1) weighing a gel matrix according to mass percentage of raw materials, dispersing the gel matrix in a proper amount of purified water, immersing for 24h, and uniformly mixing to obtain a solution A; (2) weighing antibacterial peptide HC-17 and adding the antibacterial peptide HC-17 into a proper amount of purified water, and carrying out ultrasonic treatment to obtain a solution B; (3) uniformly mixing a skin penetration enhancer and a humectants and adding the mixture into the solution A, and uniformly mixing to obtain a solution C; (4) adding the solution B into the solution C, and complementing rest purified water; and (5) slowly adding a pH regulator, and uniformly stirring to obtain the antibacterial peptide gel. The product is safe and environmentally friendly, is nonirritating and is easy to coat and clean up. The technology of the invention is simple. The antibacterial peptide gel is a product with application potential.
Description
Technical field
The present invention relates to field of medicaments, variant antibacterial peptide HC-17 gel being specifically related to a kind of Hydrophis cyanocinceus antibacterial peptide Hc-CATH and preparation method thereof.
Background technology
Cathelicidins is an important antibacterial peptide family in vertebrates body, has the antimicrobial acivity of broad-spectrum high efficacy.The cathelicidins family antibacterial peptide having now been found that all has extremely strong activity to gram-positive bacterium, gram negative bacteria, fungus, mycete, mycoplasma, chlamydia, protozoon and part envelope virus.
Cathelicidins family antibacterial peptide has bactericidal action rapidly and the feature such as mechanism of action uniqueness, by acting on microbial cell plasma membrane, plasma membrane causes pore space structure, cause plasma membrane depolarization, the change of intraor extracellular osmotic pressure, cellular content to leak and finally kill microbial cell, typically can kill antibacterial within a few minutes.
Research shows that Hydrophis cyanocinceus cathelicidins family antibacterial peptide Hc-CATH has that molecular weight is little, simple in construction, broad-spectrum high efficacy, mechanism of action unique, is difficult to cause the advantages such as microorganism drug resistance, than other antibacterial peptide, there is bigger exploitation application potential, be the good template of design novel polypeptide class antimicrobial agents.Carrying out molecular modification research with Hc-CATH for template, the antibacterial peptide HC-17 obtained has the feature of cathelicidins family antibacterial peptide, and production cost is low, specify that the variant antibacterial peptide HC-17 feasibility as novel polypeptide class antimicrobial agents.
As can be seen here, the variant antibacterial peptide HC-17 of Hydrophis cyanocinceus antibacterial peptide Hc-CATH has good application prospect, but not yet has any report about antibacterial peptide HC-17 gel series products at present.Therefore it provides a kind of efficient antibacterial peptide HC-17 gel and preparation method thereof has significance widely.
Summary of the invention
Variant antibacterial peptide HC-17 gel that it is an object of the invention to provide a kind of Hydrophis cyanocinceus antibacterial peptide Hc-CATH and preparation method thereof so that this gel has that preparation technology is simple, the effect of stable in properties.
To achieve these goals, the present invention adopts the following technical scheme that
Gel preparation method of the present invention, concrete processing step is as follows: (1) weighs gel-type vehicle by the mass percent of constitutive material, is scattered in appropriate purified water, soaks 24h, mixing, obtains solution A；(2) weighing antibacterial peptide HC-17 and join in appropriate purified water, excusing from death processes, and obtains solution B；(3) join in solution A after Percutaneous absorption enhancer and wetting agent being mixed, mixing, obtain solution C；(4) solution B is joined in solution C, supply residue purified water；(5) it is slowly added to PH regulator, stirs, i.e. obtain antibacterial peptide gel.
Gel of the present invention, its each component raw material is prepared from by following mass percent:
Each component raw material is prepared from by following mass percent:
Gel of the present invention, antibacterial peptide is variant HC-17 of Hydrophis cyanocinceus antibacterial peptide Hc-CATH.
Gel of the present invention, one or more in carbomer, poloxamer, Polyethylene Glycol, chitosan, sodium carboxymethyl cellulose, Polyethylene Glycol of gel-type vehicle.
Gel of the present invention, gel-type vehicle is Acritamer 940.
Gel of the present invention, Percutaneous absorption enhancer one in azone, Oleum menthae, Oleum Terebinthinae, Flos Caryophylli volatile oil, Borneolum Syntheticum, linoleic acid and carbamide.
Gel of the present invention, wetting agent one in propylene glycol and glycerol.
Gel of the present invention, PH regulator one in triethanolamine, sodium hydroxide, disodium hydrogen phosphate, sodium bicarbonate, ethylenediamine and lauryl amine.
In gel processing step (5) of the present invention, its pH value regulation to 5-7.
The present invention is with the variant antibacterial peptide HC-17 of Hydrophis cyanocinceus antibacterial peptide Hc-CATH as principal agent, and its antibacterial effect is to be played a role by directly sterilization, rather than bacteria growing inhibiting.Antibacterial peptide HC-17 can be by electrostatic interaction selectively acting in bacterial cell membrane, film is formed the ion channel of cross-film, destroy the integrity of film, cellular content is caused to leak, thus kill cell, there is broad spectrum antibiotic activity, and have sterilization speed fast, to thermally-stabilised, be difficult to cause the features such as antibacterial ruggedness.
The present invention is using Carbomer-940 as medicine carrying substrate, and this substrate has certain toleration to acid, alkali, alcohol etc.；It is resistant to low temperature storage and high pressure moist heat sterilization, safety non-toxic, nonirritant, there is good water solublity, the gel appearance transparent prepared, exquisiteness, easily it is coated with, the advantages such as adhesive force is strong, are a kind of preferably gel-type vehicles.
From above technical scheme, the present invention has filled up the current domestic blank of research in terms of antibacterial peptide gel, selects suitable adjuvant and simple preparation technology, is suitable for large batch of production, having great application prospect, the generation for like product provides reference.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described further, and the test method used in embodiment if no special instructions, is conventional method；The raw material that used, reagent etc., if no special instructions, the most commercially buy.
Embodiment 1, a kind of antibacterial peptide gel, its each component percentages is respectively as follows:
Above raw material is prepared as follows:
(1) weigh Acritamer 940 powder by the mass percent of constitutive material, be scattered in appropriate purified water, soak 24h, mixing, obtain solution A；
(2) weighing antibacterial peptide HC-17 powder and join in appropriate purified water, excusing from death processes, and fully mixes, obtains solution B；
(3) join in solution A after azone and glycerol being mixed, mixing, obtain solution C；
(4) solution B is joined in solution C, supply residue purified water, stirring and evenly mixing；
(5) being slowly added to triethanolamine, stir, regulate PH to 6, solution becomes gluey, i.e. obtains antibacterial peptide gel.
Embodiment 2, a kind of antibacterial peptide gel, its each component percentages is respectively as follows:
Above raw material is prepared as follows:
(1) weigh Acritamer 940 powder by the mass percent of constitutive material, be scattered in appropriate purified water, soak 24h, mixing, obtain solution A；
(2) weighing antibacterial peptide HC-17 powder and join in appropriate purified water, excusing from death processes, and fully mixes, obtains solution B；
(3) join in solution A after azone and propylene glycol being mixed, mixing, obtain solution C；
(4) solution B is joined in solution C, supply residue purified water, stirring and evenly mixing；
(5) being slowly added to sodium hydroxide, stir, regulate PH to 6, solution becomes gluey, i.e. obtains antibacterial peptide gel.
Embodiment 3, a kind of antibacterial peptide gel, its each component percentages is respectively as follows:
Above raw material is prepared as follows:
(1) weigh Acritamer 940 powder by the mass percent of constitutive material, be scattered in appropriate purified water, soak 24h, mixing, obtain solution A；
(2) weighing antibacterial peptide HC-17 powder and join in appropriate purified water, excusing from death processes, and fully mixes, obtains solution B；
(3) join in solution A after azone and propylene glycol being mixed, mixing, obtain solution C；
(4) solution B is joined in solution C, supply residue purified water, stirring and evenly mixing；
(5) being slowly added to triethanolamine, stir, regulate PH to 6, solution becomes gluey, i.e. obtains antibacterial peptide gel.
Embodiment 4, a kind of antibacterial peptide gel, its each component percentages is respectively as follows:
Preparation method and condition are with embodiment 3.
Embodiment 5, a kind of antibacterial peptide gel, its each component percentages is respectively as follows:
Preparation method and condition are with embodiment 3.
The antibacterial peptide HC-17 gel prescription of above-described embodiment is summarized in table 1, and outward appearance, pH value, viscosity, stretchability, one-tenth colloidality and the content of this gel carries out testing result such as table 2:
Table 1: gel prescription is summed up
Table 2: gel indices testing result
2 groups of gels are prepared according to the ratio described in embodiment 4, wetting agent selects propylene glycol or glycerol, often parallel 3 times of group test, the gel prepared is uniformly coated on microscope slide of the same area, under the same terms, room temperature is placed, in the different time points average percent weight loss result such as table 3 below of calculating:
3: two groups of gel average percent weight loss results of table
By above-mentioned experimental result it can be seen that with outward appearance, pH value, viscosity, stretchability, one-tenth colloidality, content and moistening effect as evaluation index, wherein embodiment 4 is most preferred embodiment.
The primary stability method that the present invention relates to medicine is as follows:
Gel prepared by Example 4 method, preserves 12h at 50 DEG C respectively by totally 3 groups, and 4 DEG C preserve 12h, and 3500 leave heart 15min, check medicine indices.
Table 4: gel primary stability testing result
As shown in Table 4, the antibacterial peptide HC-17 gel of embodiment 4 has good stability, and after every factor detects, its outward appearance, pH value, viscosity and content all do not occur significantly to change, and show this prescription and stable preparation process, can meet the needs of large-scale production.
Embodiment above describes preparation process and the implementation result of the present invention; embodiment is only used for the purpose of illustration; do not limit the scope of the invention; for those skilled in the art; under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be regarded as protection scope of the present invention.
Claims (10)
1. the preparation method of an antibacterial peptide gel, it is characterised in that concrete processing step is as follows: (1) weighs gel-type vehicle by the mass percent of constitutive material, is scattered in appropriate purified water, soaks 24h, mixing, obtains solution A；(2) weighing antibacterial peptide HC-17 and join in appropriate purified water, excusing from death processes, and obtains solution B；(3) join in solution A after Percutaneous absorption enhancer and wetting agent being mixed, mixing, obtain solution C；(4) solution B is joined in solution C, supply residue purified water；(5) it is slowly added to PH regulator, stirs, i.e. obtain antibacterial peptide gel.
2. according to the method preparing antibacterial peptide gel described in right 1, it is characterised in that each component raw material is prepared from by following mass percent:
3. according to the preparation method described in right 2, it is characterised in that each component raw material is prepared from by following mass percent:
Preparation method the most according to claim 2, it is characterised in that: antibacterial peptide is the variant antibacterial peptide HC-17 of Hydrophis cyanocinceus antibacterial peptide Hc-CATH.
Preparation method the most according to claim 2, it is characterised in that: one or more in carbomer, poloxamer, Polyethylene Glycol, chitosan, sodium carboxymethyl cellulose, Polyethylene Glycol of gel-type vehicle.
Preparation method the most according to claim 2, it is characterised in that: gel-type vehicle is Acritamer 940.
Preparation method the most according to claim 2, it is characterised in that: Percutaneous absorption enhancer one in azone, Oleum menthae, Oleum Terebinthinae, Flos Caryophylli volatile oil, Borneolum Syntheticum, linoleic acid and carbamide.
Preparation method the most according to claim 2, it is characterised in that: wetting agent one in propylene glycol and glycerol.
Preparation method the most according to claim 2, it is characterised in that: PH regulator one in triethanolamine, sodium hydroxide, disodium hydrogen phosphate, sodium bicarbonate, ethylenediamine and lauryl amine.
The most according to claim 1 prepare antibacterial peptide gel method, it is characterised in that: in step (5), pH value regulation to 5-7.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510001466.8A CN105816853A (en) | 2015-01-04 | 2015-01-04 | Modified antibacterial peptide and preparation of gel |
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| CN201510001466.8A CN105816853A (en) | 2015-01-04 | 2015-01-04 | Modified antibacterial peptide and preparation of gel |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115006513A (en) * | 2022-06-13 | 2022-09-06 | 刘瑶 | A kind of antibacterial peptide hydrogel liniment and preparation method and application thereof |
Citations (4)
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|---|---|---|---|---|
| CN1583165A (en) * | 2004-05-25 | 2005-02-23 | 吉林大学 | Gel gent of Chiense forest frog bactericidal peptide |
| CN103156804A (en) * | 2011-12-13 | 2013-06-19 | 重庆医药工业研究院有限责任公司 | Oxybutynin transdermal gel and preparation method thereof |
| CN103665111A (en) * | 2012-09-07 | 2014-03-26 | 山东国际生物科技园发展有限公司 | Transformed body HC-15 of antimicrobial peptide Hc-CATH (cathelicidins) of hydrophis cyanocinctus and preparation method and application of transformed body |
| WO2014093297A1 (en) * | 2012-12-10 | 2014-06-19 | The Regents Of The University Of California | Cathelicidin as novel inflammatory bowel disease marker and therapy for colitis-associated intestinal fibrosis |
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2015
- 2015-01-04 CN CN201510001466.8A patent/CN105816853A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1583165A (en) * | 2004-05-25 | 2005-02-23 | 吉林大学 | Gel gent of Chiense forest frog bactericidal peptide |
| CN103156804A (en) * | 2011-12-13 | 2013-06-19 | 重庆医药工业研究院有限责任公司 | Oxybutynin transdermal gel and preparation method thereof |
| CN103665111A (en) * | 2012-09-07 | 2014-03-26 | 山东国际生物科技园发展有限公司 | Transformed body HC-15 of antimicrobial peptide Hc-CATH (cathelicidins) of hydrophis cyanocinctus and preparation method and application of transformed body |
| WO2014093297A1 (en) * | 2012-12-10 | 2014-06-19 | The Regents Of The University Of California | Cathelicidin as novel inflammatory bowel disease marker and therapy for colitis-associated intestinal fibrosis |
Non-Patent Citations (2)
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| 周广芬等: "甘草酸单铵凝胶剂的制备研究", 《中药材》 * |
| 苏华等: "均匀设计法优化复方熊果苷凝胶的基质处方", 《中国药师》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115006513A (en) * | 2022-06-13 | 2022-09-06 | 刘瑶 | A kind of antibacterial peptide hydrogel liniment and preparation method and application thereof |
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Effective date of registration: 20161201 Address after: 264670 high tech Zone, Shandong, Yantai science and technology Avenue, No. 39 Applicant after: Shandong International Biotechnology Park Development Co., Ltd. Applicant after: Lvye Investment Group Co., Ltd. Address before: 264670 high tech Zone, Shandong, Yantai science and technology Avenue, No. 39 Applicant before: Shandong International Biotechnology Park Development Co., Ltd. |
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Application publication date: 20160803 |
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