CN105567699A - Albumin variants - Google Patents
Albumin variants Download PDFInfo
- Publication number
- CN105567699A CN105567699A CN201510731661.6A CN201510731661A CN105567699A CN 105567699 A CN105567699 A CN 105567699A CN 201510731661 A CN201510731661 A CN 201510731661A CN 105567699 A CN105567699 A CN 105567699A
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- Prior art keywords
- albumin
- fragment
- variant
- fusion polypeptide
- hsa
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/76—Albumins
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- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/31—Fusion polypeptide fusions, other than Fc, for prolonged plasma life, e.g. albumin
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Abstract
The invention relates to albumin variants. The present invention relates to variants of a parent albumin having altered plasma half-life compared with the parent albumin. The present invention also relates to fusion polypeptides and conjugates comprising said variant albumin.
Description
The application is the divisional application that the denomination of invention submitted on November 1st, 2010 is " albumin variants ", application number is the application for a patent for invention of " 201080060322.5 ".
Relate to sequence table
The application contains the sequence table of computer-reader form, and it is incorporated to herein by carrying stating.
Background of invention
Technical field
The present invention relates to compared to albumin, its fragment or the fusion polypeptide comprising albumin or its fragment, there is albumin variants or its fragment of half life change, or comprise the fusion polypeptide of variant albumin or its fragment.
Background technology
Albumin is the natural albumen seen in mammalian plasma, and in blood plasma, it is the abundantest albumen.It is maintaining in the desirable osmotic pressure of blood, and transports in blood flow in many kinds of substance and play a significant role.
Comprise people, pig, mouse, rat, rabbit and goat from multiple species and characterize albumin, and they share high degree of sequence and structural homology.
Albumin in vivo with its acceptor, namely neonatal Fc receptor (FcRn) " Brambell " combines, and this interaction known is important for albuminous plasma half-time.FcRn is embrane-associated protein, expresses in various kinds of cell and organization type.Find that FcRn saves albumin and degrades (RoopenianD.C. and Akilesh, S. (2007), Nat.Rev.Immunol7,715-725.) from born of the same parents.FcRn participates in Mammals as maintained high-caliber IgG and albuminous bi-functional molecule in serum human.
Although characterized FcRn immunoglobulin (Ig) (IgG) in prior art to interact, still insufficient to the interactional sign of FcRn-albumin.Main FcRn binding site is positioned at DIII (381-585).Andersen etc. (2010) .ClinicalBiochemistry43,367 – 372.Data show that IgG and albumin miscoordination are incorporated into different loci (Andersen etc. (2006), Eur.J.Immunol36,3044-3051 on FcRn; Chaudhury etc. (2006), Biochemistry45,4983-4990).
Known mouse FcRn combines the IgG from mouse and the mankind, and people FcRn shows more fastidious (Ober etc. (2001) Int.Immunol13,1551-1559).Andersen etc. (2010) .JournalofBiologicalChemistry285 (7): 4826-36 describes people and mouse FcRn respectively to the avidity of mouse and human albumin (all possible combination).Do not observe albumin from arbitrary species to any combination of arbitrary acceptor in physiological pH.At acid pH, observe binding affinity and there are 100 times of differences.In all cases, be additive properties from the albumin of arbitrary species and the combination of IgG to two kinds of acceptors.
Human serum albumin (HSA) is fully characterized by 585 amino acid whose polypeptide, its sequence is found in Peters, T., Jr. (1996) AllaboutAlbumin:Biohemistry, GeneticsandMedical, Applicationspp10, AcademicPress, Inc., Orlando (ISBN0-12-552110-3).It has and combines the characteristic of its acceptor FcRn, and wherein it combines at pH6.0, but does not combine at pH7.4.
Find that the plasma half-time of HSA is about 19 days.Identified the natural variant (Peach, R.J. and Brennan, S.0., (1991) BiochimBiophysActa.1097:49-54) with lower plasma half-time, it has replacement D494N.This is substituted in this variant and generates N-glycosylation site, and this site is not present in Wild-type albumin.Do not know whether this glycosylation or amino acid change cause the change of plasma half-time.
Albumin has long plasma half-time, and because this characteristic, has proposed to use it for drug delivery.Albumin is puted together in pharmaceutically useful compound (WO2000/69902A), and found that this conjugate keeps albuminous long plasma half-time.The conjugate plasma half-time generally remarkable plasma half-time being longer than useful therapeutic compound itself of gained.
In addition, by the peptide (WO2001/79271A and WO2003/59934A) that Albumin fusion is useful in treatment, this has caused fusions to have the activity of peptide useful in described treatment and the plasma half-time significantly longer relative to the plasma half-time of peptide useful in described treatment itself usually.
Otagiri etc. (2009), Biol.Pharm, Bull.32 (4), 527-534 discloses known 77 albumin variants, and wherein 25 see territory III.Show and lacked the half life (Andersen etc. (2010), ClinicalBiohemistry43,367-372) that last 175 the amino acid whose natural variant of carboxyl terminal have minimizing.Iwao etc. (2007) use mouse model to have studied the half life of the human albumin variant of natural appearance, and find that K541E and K560E has the half life of minimizing, E501K and E570K has the half life of increase, and K573E to half life almost without effect (Iwao etc. (2007) B.B.A.ProteinsandProteomics1774,1582-1590).
Galliano etc. (1993) Biochim.Biophys.Acta1225,27-32 disclose natural variant E505K.Minchiotti etc. (1990) disclose natural variant K536E.Minchiotti etc. (1987) Biochim.Biophys.Acta916,411-418 disclose natural variant K574N.Takahashi etc. (1987) Proc.Natl.Acad.Sci.USA84,4413-4417 disclose natural variant D550G.Carlson etc. (1992) .Proc.Nat.Acad.Sci.USA89,8225-8229 disclose natural variant D550A.
Albumin has the ability in conjunction with multiple ligands, and these parts and albumin associate (associated complex) mutually.This characteristic has been utilized to have Non-covalent binding in the plasma half-time of the medicine of albuminous ability to extend.This also realizes by tool hardly or the pharmaceutically useful compound of albumin bound characteristic of not having being combined with the module with albumin bound characteristic.See Review literature Kratz (2008) .JournalofControlledRelease132,171-183 and reference wherein.
Albumin is for the preparation of pharmaceutically useful compound, and wherein this kind of prepared product can such as but not limited to albuminous nano particle or micron particle.In these examples, pharmaceutically useful compound or sending of the mixture of compound can benefit from the change of albumin to its receptor affinity, wherein for delivery means, have shown described beneficial compound and have associated mutually with albumin.
It is unclear that formation associated complex (such as, but not limited to
the Biochem.J.1995 such as KurtzhalsP; 312:725-731), what the plasma half-time of conjugate or fusion polypeptide determined by, but it is like be albumin and the result of the combination of the pharmaceutically useful compound/polypeptide selected.In order to certain drug can be designed according to the feature of the indication of intention treatment, expect the plasma half-time that can control given albumin conjugate, associated complex or Albumin fused polypeptide, thus make to obtain plasma half-time longer or shorter compared with the plasma half-time given by the component of described associated complex, conjugate or fusions.
Known albumin accumulates concurrent raw katabolism in tumour, also shows it and accumulates in the joint of the inflammation of patient with rheumatoid arthritis.See Review literature and the reference wherein of Kratz (2008) JournalofControlledRelease132,171-183.Estimate that the HSA variant increased for FcRn avidity can be favourable for sending of pharmaceutically useful compound.
Even can expect obtain to FcRn almost without or without combination albumin variants, for providing shorter half life or controlled serum pharmacokinetic, as (2009) J.Nucl.Med. such as Kenanova; 50 (Supplement2): 1582).
Summary of the invention
The invention provides the albuminous variant of parent of the characteristic compared with its parent with improvement.Specifically, the invention provides the albuminous variant of parent of the plasma half-time compared with its parent with change.
The present invention relates to albumin variants or its fragment of the albuminous separation of parent, or comprise the fusion polypeptide of variant albumin or its fragment, it is in the position 417 of the mature polypeptide corresponding to SEQIDNO:2, 440, 464, 490, 492, 493, 494, 495, 496, 499, 500, 501, 503, 504, 505, 506, 510, 535, 536, 537, 538, 540, 541, 542, 550, 573, 574, 575, 577, 578, 579, 580, 581, one or more (several) position of 582 and 584 comprises change, wherein said variant is not by having replacement D494N, E501K, K541E, D550G, A, the variant of the SEQIDNO:2 composition of K573E or K574N.
Change in one or more position independently selected from replacement, insertion and disappearance, wherein preferably can replace.
The invention still further relates to the polynucleotide of the separation of described variant of encoding; Relate to the nucleic acid construct, carrier and the host cell that comprise described polynucleotide; And relate to the method producing described variant.
The invention still further relates to the conjugate or associated complex that comprise variant albumin of the present invention or its fragment, and useful treatment module, or relate to the fusion polypeptide comprising variant albumin of the present invention or its fragment and fusion partner polypeptide (fusionpartnerpolypeptide).
The invention further relates to composition, it comprises variant albumin, its fragment, comprises the fusion polypeptide of variant albumin or its fragment, or comprise the conjugate of variant albumin of the present invention or its fragment, or comprise the associated complex of variant albumin of the present invention or its fragment.Described composition is preferably pharmaceutical compositions.
The invention further relates to pharmaceutical compositions, it comprises variant albumin, its fragment, comprise the fusion polypeptide of variant albumin or its fragment, or comprise the conjugate of described variant albumin or its fragment, or comprise the associated complex of described variant albumin or its fragment, wherein said variant albumin, its fragment, comprise the fusion polypeptide of variant albumin or its fragment, or comprise the conjugate of described variant albumin or its fragment, or comprise the associated complex of variant albumin or its fragment and HSA or its fragment, comprise the fusion polypeptide of HSA or its fragment, or the corresponding plasma half-time comprising the HSA of HSA or its fragment or the conjugate of its fragment or associated complex is compared, there is the plasma half-time of change.
Accompanying drawing is sketched
The restriction figure of Fig. 1 Explicit Expression plasmid pDB4082.
The restriction figure of Fig. 2 Explicit Expression plasmid pDB2305.
The restriction figure of Fig. 3 Explicit Expression plasmid pDB4005.
10 μMs of albumin are injected SPR induction spectrum (sensorgram) of gained by Fig. 4 display for shFcRn, the HSA (A3782) of the not fatty acids of HSA (JTA)=obtain from Sigma-Aldrich, HSA (Novozymes)=commercial recombination human serum albumin (RECOMBUMIN).
Fig. 5 shows shFcRn-GST and combines (100-0.045 μ g/ml) the ELISA of human serum albumin (HSA) variant.PH6.0 and pH7.4 that be combined in of WT, D494N, D494Q and D494A carries out.PH6.0 and pH7.4 that be combined in of WT, D494N, D494N/T496A and T496A carries out.PH6.0 and pH7.4 that be combined in of WT, E495Q and E495A carries out.
Fig. 6 shows the HSA variant of 0.2 μM to the representativeness induction spectrum of the combination of immobilized shFcRn (~ 4600RU).WT, D494N, D494Q, D494A, D494N/T496A and T496A.
Fig. 7 shows the HSA variant of 1 μM to the representativeness induction spectrum of the combination of immobilized shFcRn (~ 1400RU).WT, D494N, D494Q, D494A, D494N/T496A and T496A.
Fig. 8 display is based on being such as shown in the twice independently SPR experiment of (A) Fig. 6 with (B) Fig. 7, the relative combination of HSA variant compared with WT.
Fig. 9 shows ELISA:(A) at pH6.0, shFcRn to the albuminous combination from people, donkey, ox, sheep, goat and rabbit.(B) at pH6.0, shFcRn to the albuminous combination from cavy, hamster, rat and chicken.(C) at pH7.4, shFcRn to the albuminous combination from people, donkey, ox, sheep, goat and rabbit.(D) at pH7.4, shFcRn to the albuminous combination from cavy, hamster, rat and chicken.(E) different albuminous relative combination.The relative combination of human albumin to shFcRn is defined as 1.0.The representative of ELISA value repeats the mean value of testing.
Figure 10 shows SPR: at pH6.0 and pH7.4, shFcRn-GST to the albuminous combination from several species.The display of representative induction spectrum is to 5.0 μMs of albuminous combinations from different plant species: (A) people, (B) donkey, (C) ox, (D) goat, (E) sheep, (F) rabbit, (G) dog, (H) cavy, (I) hamster, (J) rat, (K) mouse and (L) chicken.The shFcRn (~ 2100RU) that described albumin variants marks for immobilized GST injects.Be infused in 25 DEG C to carry out with the speed of 40 μ l/min.
Figure 11 shows the SPR induction spectrum of selected HSA mutant and comparing of wild-type HSA.By (A) WT and P499A of 20 μMs, (B) WT and K500A, (C) WT and K536A, (D) WT and P537A, and (E) WT and K538A, and (F) WT and K537A injects at pH6.0 (~ 1500RU) for immobilized shFcRn.
Figure 12 shows the SPR induction spectrum of HSA mutant and comparing of WTHSA.By (A) WT and K573A of 10 μMs, (B) WT and K573C, (C) WT and K573F, (D) WT and K573G, and (E) WT and K573L, and (F) WT and K573M, (G) WT and K573Q, (H) WT and K573R, and (I) WT and K573T, and (J) WT and K573V injects at pH5.5 and pH7.4 for immobilized shFcRn.Be infused in 25 DEG C to carry out with the flow velocity of 80 μ l/min.
Figure 13 shows the SPR induction spectrum of HSA mutant and comparing of wild-type HSA.By (A) WT and K573D of 10 μMs, (B) WT and K573E, (C) WT and K573H, (D) WT and K573I, and (E) WT and K573N, and (F) WT and K573P, (G) WT and K573S, (H) WT and K573*, and (I) WT and K573W, and (J) WT and K573Y injects at pH5.5 and pH7.4 for immobilized shFcRn.Be infused in 25 DEG C to carry out with the flow velocity of 80 μ l/min.
Figure 14 shows the SPR induction spectrum of HSA mutant and comparing of wild-type HSA.By (A) WT and E492G+K538H+K541N+E542D of 20 μMs, (B) WT and E492T+N503K+K541A, (C) WT and E492P+N503K+K541G+E542P, (D) WT and E492H+E501P+N503H+E505D+T506S+T540S+K541E, and (E) WT and A490D+E492T+V493L+E501P+N503D+A504E+E505K+T506F+K541D, and (F) WT and E492G+V493P+K538H+K541N+E542D injects for immobilized shFcRn at pH6.0.Be infused in 25 DEG C to carry out with the flow velocity of 80 μ l/min.
Figure 15 shows the SPR induction spectrum of HSA mutant and comparing of wild-type HSA.(A) WT of 20 μMs, (B) H440Q, (C) H464Q, (D) H510Q and (E) H535Q are injected at pH6.0 for immobilized shFcRn.Be infused in 25 DEG C to carry out with the flow velocity of 80 μ l/min.
Figure 16 shows the SPR induction spectrum of HSA mutant K500E and comparing of wild-type HSA.The HSA mutant K500E of 10 μMs is injected at pH5.75 for immobilized shFcRn.Be infused in 25 DEG C to carry out with the flow velocity of 30 μ l/min.
The restriction figure of Figure 17 Explicit Expression plasmid pDB3017.
The restriction figure of Figure 18 Explicit Expression plasmid pDB3021.
The restriction figure of Figure 19 Explicit Expression plasmid pDB3056.
The restriction figure of Figure 20 Explicit Expression plasmid pDB3165.
The restriction figure of Figure 21 Explicit Expression plasmid pDB4172.
The restriction figure of Figure 22 Explicit Expression plasmid pDB4267.
The restriction figure of Figure 23 Explicit Expression plasmid pDB4285.
Figure 24 shows the GP-HPLC chromatogram that WTHSA and mutant K573PHRP conjugate are analyzed for shFcRn.On TSKG3000SWXL post (TosohBioscience), the injection of 25 μ L is carried out as described in materials and methods.
Figure 25 show SDSPAGE be separated continue with fluorescein put together albuminous naked eyes (A) and ultraviolet (B) detection.HSA::F5M (swimming lane 1), K573P::F5M (swimming lane 2) and rHA standard (swimming lane 3).
Figure 26 shows the shFcRn binding characteristic of HSA variant.By WTrHA and E492T of 10 μMs (A), WTrHA and D494N/E495Q/T496A (B), WTrHA and N503D (C), WTrHA and N503K (D), WTrHA and E492T/N503D (E), WTrHA and E495Q/T496A (F), WTrHA and K538H (G), WTrHA and E492D (H) injects for immobilized shFcRn at pH5.5.
Figure 27 shows the shFcRn binding characteristic of HSA variant.WTrHA and K541A of 10 μMs (I) and WTrHA and K541N (J) are injected for immobilized shFcRn at pH5.5.
Figure 28 shows the competitive binding of K573A and K573P, by shFcRn (100nM) to be injected separately for immobilized HSA (~ 2500RU) at pH6.0 or with different HSAK573A and K573P preincubation of measuring after inject and measure.
Figure 29 shows the competitive binding of HSA-FLAG variant, measures by for immobilized HSA (~ 2500RU) at pH6.0 shFcRn (100nM) injecting separately or together injected from the HSA-FLAG variant of different amount.
Figure 30 shows the competitive binding of HSA-IL1Ra variant, measures by for immobilized HSA (~ 2500RU) at pH6.0 shFcRn (100nM) injecting separately or together injected from the HSA-IL1Ra variant of different amount.
Figure 31 shows the competitive binding of HSA variant that scFv merges, and measures by be injected separately by shFcRn (100nM) for immobilized HSA (~ 2500RU) at pH6.0 or together inject from (A) scFv-HSA-FLAG variant of different amount or (B) HSA-scFv-FLAG variant.
Figure 32 shows HSA, and single, dual and triple mutant variant is to the combination of shFcRn.10 of often kind of HSA variant μMs of samples are injected for immobilized shFcRn at pH5.5 or pH7.4.
Detailed Description Of The Invention
The present invention relates to albumin variants or its fragment of the albuminous separation of parent, or comprise the fusion polypeptide of variant albumin or its fragment, it is in the position 417 of the mature polypeptide corresponding to SEQIDNO:2, 440, 464, 490, 492, 493, 494, 495, 496, 499, 500, 501, 503, 504, 505, 506, 510, 535, 536, 537, 538, 540, 541, 542, 550, 573, 574, 575, 577, 578, 579, 580, 581, one or more (several) position of 582 and 584 comprises change, wherein said variant is not by having replacement D494N, E501K, K541E, D550G, A, the variant of the SEQIDNO:2 composition of K573E or K574N.
Change in one or more position independently selected from replacement, insertion and disappearance, wherein preferably can replace.
Definition
Variant: term " variant " means by the one or more changes in one or more (several) position, the polypeptide namely replacing, insert and/or lack and derive from parent's albumin.Replace and mean to substitute with different aminoacids the amino acid occupying certain position; Disappearance means to remove the amino acid occupying certain position; And it is one or more to insert the aminoacid addition meaning and then to occupy certain position, a preferred 1-3 amino acid.
Mutant: term " mutant " means the polynucleotide of encode variant.
Wild-type albumin: term " wild-type " (WT) albumin means to have the albumin with the natural identical aminoacid sequence seen in animal or human.
Parent or parent's albumin: term " parent " or " parent's albumin " mean to cause through manpower to change with the albumin producing albumin variants of the present invention.Described parent can be (wild-type) polypeptide or its allelotrope (allele) of natural appearance, or even its variant.
FcRn and shFcRn: term " FcRn " means people's neonatal Fc receptor (FcRn).ShFcRn is the Soluble recombinant versions of FcRn.
SmFcRn: term " smFcRn " is the Soluble recombinant versions of mouse neonatal Fc acceptor.
The variant be separated: term " variant of separation " means to modify also wholly or in part from the variant of at least one component separating of appearance natural with it through manpower.In one aspect, determining that this variant is at least 1% pure according to SDS-PAGE or GP-HPLC, such as, is at least 5% pure, being at least 10% pure, is at least 20% pure, is at least 40% pure, being at least 60% pure, is at least 80% pure, and is at least 90% pure.
Substantially the variant that (substantially) is pure: term " substantially pure variant " means a kind of prepared product, it comprises at the most 10%, at the most 8%, at the most 6%, at the most 5%, at the most 4%, at the most 3%, at the most 2%, at the most 1%, and other peptide materials of combining of 0.5% or restructuring natural with it by weight at the most.Preferably, described variant is that the total peptide material weighing scale at least 92% be present in this prepared product is pure, and such as at least 94% is pure, and at least 95% is pure, at least 96% is pure, and at least 97% is pure, and at least 98% is pure, at least 99% is pure, and at least 99.5% is pure, and 100% is pure.Variant of the present invention is preferably substantially pure form.Its by, such as, prepare this variant with known recombination method and purification process and reach.
Mature polypeptide: term " mature polypeptide " means through translation and any posttranslational modification, as the polypeptide of the final form after N-terminal processing, C-terminal brachymemma, glycosylation, phosphorylation etc.In one aspect, described mature polypeptide is the amino acid/11 to 585 of SEQIDNO:2, comprises any posttranslational modification.
Mature polypeptide encoded sequence: term " mature polypeptide encoded sequence " means the polynucleotide of encoding mature albumin polypeptide.In one aspect, described mature polypeptide encoded sequence is the Nucleotide 1 to 1758 of SEQIDNO:1.
Sequence iden: the dependency between two aminoacid sequences or between two nucleotide sequences is by parameter " sequence iden " described by.
For the present invention, degree of sequence identity between two aminoacid sequences uses Needleman-Wunsch algorithm (Needleman and Wunsch, 1970, J.Mol.Biol.48:443-453) determine, as EMBOSS software package (EMBOSS: European Molecular Biology Open software group (TheEuropeanMolecularBiologyOpenSoftwareSuite), Rice etc., 2000, TrendsGenet.16:276-277) Needle program, is preferably and performs in 3.0.0 version or version afterwards.Optional parameter used is gap open penalty (gapopenpenalty) 10, gap extension penalty (gapextensionpenalty) 0.5, and EBLOSUM62 (the EMBOSS version of BLOSUM62) substitution matrix.Applying marking is that the Needle of " the longest identity " exports (use-nobrief option obtain) as percentage identities and to calculate as follows:
(identical residue × 100)/(in comparison length-comparison breach sum)
For the present invention, degree of sequence identity between two deoxyribonucleotide sequence uses Needleman-Wunsch algorithm (Needleman and Wunsch, 1970, on seeing) determine, as EMBOSS software package (EMBOSS: European Molecular Biology Open software group (TheEuropeanMolecularBiologyOpenSoftwareSuite), Rice etc., 2000, on seeing) Needle program, be preferably and perform in 3.0.0 version or version afterwards.Optional parameter used is gap open penalty 10, gap extension penalty 0.5, and EDNAFULL (the EMBOSS version of NCBINUC4.4) substitution matrix.Applying marking is " the longest identity " Needle export (use-nobrief option obtain) as percentage identities and to calculate as follows:
(identical deoxyribonucleotide × 100)/(in comparison Chang Du – comparison breach sum)
Fragment: term " fragment " means from albuminous amino and/or carboxyl terminal, and/or albuminous inner area has one or more (several) aminoacid deletion, and retain the polypeptide being incorporated into the ability of FcRn.Fragment origin can come from the sequence composition that of HSA do not interrupt, or it can comprise the sequence that two or more derive from HSA.Fragment of the present invention has the size exceeding about 20 amino-acid residues, preferably greater than 30 amino-acid residues, more preferably above 40 amino-acid residues, more preferably above 50 amino-acid residues, more preferably above 75 amino-acid residues, more preferably above 100 amino-acid residues, more preferably above 200 amino-acid residues, more preferably above 300 amino-acid residues, even more preferably above 400 amino-acid residues, and most preferably more than the size of 500 amino-acid residues.
Allelic variant (allelicvariant): term " allelic variant " means any two or more Alternative Form of the gene occupying phase syntenic genes seat.Allelic variation is occurred natively by sudden change, and can cause the polymorphism in population.Transgenation can be the polypeptide of reticent (unchanged in the polypeptide of the coding) aminoacid sequence with change of maybe can encoding.The allelic variant of polypeptide is the polypeptide of being encoded by the allelic variant of gene.
Encoding sequence: term " encoding sequence " means directly to specify it to translate the polynucleotide of the aminoacid sequence of the polypeptide product obtained.The border of encoding sequence is determined by opening frame usually, described in open frame and usually start with ATG initiator codon or alternative initiator codon such as GTG and TTG, and with terminator codon as TAA, TAG and TGA terminate.Encoding sequence can be DNA, cDNA, synthesis or restructuring polynucleotide.
CDNA: term " cDNA " mean can by reverse transcription from derive from eukaryotic maturation, DNA molecular prepared by the mRNA molecule of montage.CDNA lacks the intron sequences that can be present in corresponding gene group DNA.Initial (initial), elementary rna transcription thing are the precursors of mRNA, and it processes (comprising montage) then as the mRNA appearance of ripe montage by a series of step.
Nucleic acid construct: term " nucleic acid construct " means the nucleic acid molecule of strand or double-strand, described nucleic acid molecule is separated from naturally occurring gene, or to be modified in the mode originally not being present in (nototherwiseexist) occurring in nature with the section containing nucleic acid or described nucleic acid molecule by described nucleic acid molecule be synthesize.When described nucleic acid construct contains the regulating and controlling sequence of expressing needed for encoding sequence of the present invention, term nucleic acid construct and term " expression cassette " synonym.
Regulating and controlling sequence (controlsequence): the polynucleotide that term " regulating and controlling sequence " means code book invention variant express necessary all components.Each regulating and controlling sequence can be natural or external source for the polynucleotide of the described variant of coding, or each regulating and controlling sequence is for can be natural or external source each other.These regulating and controlling sequences include but not limited to leader sequence, polyadenylation se-quence, propeptide sequence, promotor, signal peptide sequence and transcription terminator.Minimum situation, the termination signal that regulating and controlling sequence comprises promotor and transcribes and translate.Regulating and controlling sequence can provide together with the joint for introducing specific restriction sites, and described specific restriction sites promotes the connection of regulating and controlling sequence in the polynucleotide encoding district of encode variant.
Be operably connected: term " is operably connected " and means such configuration, wherein regulating and controlling sequence is placed in the appropriate location of the encoding sequence relative to polynucleotide, make regulating and controlling sequence instruct the expression of encoding sequence.
Express: term " expressions " comprises any step relating to variant generation, it include but not limited to transcribe, post transcriptional modificaiton, translation, posttranslational modification and secretion.
Expression vector: term " expression vector " means DNA molecular that is linear or ring-type, and it comprises the polynucleotide of encode variant, and be operably connected with for being used for its additional nucleotides of expressing.
Host cell: term " host cell " means for being susceptible (susceptible) any cell type with the conversion, transfection, transduction etc. of the nucleic acid construct or expression vector that comprise polynucleotide of the present invention.The spawn of the parental cell incomplete same with parental cell due to the sudden change occurred in a replication process contained in term " host cell ".
Plasma half-time: ideally, plasma half-time should be determined in vivo in suitable individual.But, due to such elapsed time, cost intensive, and inevitably exist the ethics problem brought is tested to animal and human, therefore expect to use external test to determine whether plasma half-time extends or shorten.Known albumin is important to the combination of its acceptor FcRn for plasma half-time, and the association between receptors bind and plasma half-time is the plasma half-time that the more high-affinity of albumin to its acceptor causes more growing.Therefore for the present invention, the higher avidity of albumin to FcRn is regarded as indicating the plasma half-time increased, and albumin is to the plasma half-time being regarded as instruction minimizing compared with low-affinity of its acceptor.
In the application and claims, be combined the term avidity and statement " stronger " or " more weak " of albumin to its acceptor FcRn describe.Therefore, be understood that to have to be considered the molecule of FcRn higher affinity compared with HSA be incorporated into FcRn strongly compared with HSA, and have to be considered compared with the molecule of low-affinity FcRn compared with HSA be weaker incorporated into FcRn compared with HSA.
Term " longer plasma half-time " or " shorter plasma half-time " and similar statement are interpreted as relative to corresponding parent's albumin molecule.Therefore, for albumin variants of the present invention, longer plasma half-time means described variant and has identical sequence but be not contained in corresponding to 417,440,464,490,492,493,494,495,496,499,500,501,503,504,505,506,510,535,536,537,538,540,541,542,550,573,574,575,577,578,579,580,581,582 to compare with the corresponding albumin of change of the position of 584 and have longer plasma half-time in SEQIDNO:2.
Variant naming rule:
For the present invention, the mature polypeptide be disclosed in SEQIDNO:2 is used for determine amino-acid residue corresponding in other albumin.Other albuminous aminoacid sequences and the mature polypeptide be disclosed in SEQIDNO:2 are compared, and based on this comparison, use as EMBOSS wraps (EMBOSS:TheEuropeanMolecularBiologyOpenSoftwareSuite, Rice etc., 2000, TrendsGenet.16:276-277) Needleman-Wunsch algorithm (Needleman and Wunsch performed in Needle program (preferred version 3.0.0 or renewal), 1970, J.Mol.Biol.48:443-453) amino acid position number that in the mature polypeptide disclosed in SEQIDNO:2, any amino-acid residue is corresponding is determined.
In other albumin, identify that corresponding amino-acid residue confirms by using the multiple peptide sequence of " ClustalW " (Larkin etc., 2007, Bioinformatics23:2947-2948) comparison.
(Lindahl and Elofsson when other polypeptide (or albumen) cannot detect the degree of its relation from the mature polypeptide difference of SEQIDNO:2 to traditional comparison based on sequence, 2000, J.Mol.Biol.295:613-615), other can be used by sequence comparison algorithm.Higher susceptibility can use the search utility adopting the probability of peptide family performance (probabilisticrepresentation) (sequence type) to carry out search database to obtain in based on the search of sequence.For example, PSI-BLAST program produces sequence type by (iterative) database search process of iteration, and can detect homologue (Atschul etc., 1997, NucleicAcidsRes.25:3389-3402) far away.When the family of polypeptide or superfamily have one or more representative in Protein structure databases, even higher susceptibility can be reached.Program is as GenTHREADER (Jones1999, J.Mol.Biol.287:797-815; McGuffin and Jones, 2003, Bioinformatics19:874-881) use information from multiple source (PSI-BLAST, secondary structure prediction (secondarystructureprediction), structure alignment sequence type (structuralalignmentprofile) and dissolve gesture (solvationpotential)) as the input of the neural network of the structure folding (structuralfold) to predicted query sequence (querysequence).Similarly, the method for Gough etc., 2000, J.Mol.Biol.313:903-919 can be used for the sequence of unknown structure to compare being present in the superfamily model in SCOP database.These comparisons can be used in generating the Homology model about polypeptide then, and above-mentioned model can use the multiple instrument developed for this purpose to be evaluated with regard to accuracy.
For the protein of known structure, several instruments available and resource are for giving (retrieve) and generating structure comparison for change.For example, the protein of SCOP superfamily has been carried out structure alignment, and these comparisons can to obtain and Downloadable.Two or more protein structures can use many algorithms, as distance comparison matrix (distancealignmentmatrix) (Holm and Sander, 1998, Proteins33:88-96) or combination extend (combinatorialextension) (Shindyalov and Bourne, 1998, ProteinEngineering11:739-747) compare, and the execution of these algorithms can in addition for inquiring about the structural database with object construction, to find possible structural homologue (such as Holm and Park, 2000, Bioinformatics16:566-567).
When describing albumin variants of the present invention, in order to reference conveniently, have employed following nomenclature.Use general IUPAC single-letter or three letter amino acid abbreviation.
Replace.For aminoacid replacement, use following nomenclature: initial, position, substituted amino acid.Correspondingly, such as Threonine called after " Thr226Ala " or " T226A " is replaced at 226 with L-Ala.Multiple mutation plus sige ("+") separates, such as, " Gly205Arg+Ser411Phe " or " G205R+S411F ", representative uses arginine (R) substituted glycinic acid (G) respectively at 205 and 411, and replaces Serine (S) with phenylalanine (F).Accompanying drawing also uses ("/"), such as " E492T/N503D ", and this should regard as and can exchange with ("+").
disappearance.For aminoacid deletion, employ following nomenclature: initial, position *.Correspondingly, glycine called after " Gly195* " or " G195* " is lacked in position 195.Multiple disappearance is separated by plus sige ("+"), such as " Gly195*+Ser411* " or " G195*+S411* ".
insert.For aminoacid insertion, employ following nomenclature: initial, position, initial, the amino acid of insertion.Therefore, after the glycine of position 195, Methionin called after " Gly195GlyLys " or " G195GK " is inserted.Multiple amino acid whose insertion called after [initial, position, initial, the amino acid #1 of insertion, the amino acid #2 of insertion; Etc.].Such as, after the glycine of position 195, Methionin is inserted and L-Ala is designated as " Gly195GlyLysAla " or " G195GKA ".
In the case, add lowercase by the location number of the amino-acid residue before the amino-acid residue inserted and the amino-acid residue inserted is numbered.Therefore, in a upper example, sequence is:
| Parent: | Variant: |
| 195 | 195 195a 195b |
| G | G- K- A |
multiple change.Comprise the variant of multiple change by plus sige ("+") separately, such as " Arg170Tyr+Gly195Glu " or " R170Y+G195E " representative replaces arginine and glycine with tyrosine and L-glutamic acid respectively in position 170 and 195.
different replacements.When can import different replacement a position, different replacements is separated by comma, and such as " Arg170Tyr, Glu " representative replaces arginine in position 170 with tyrosine or L-glutamic acid.Therefore, " Tyr167Gly, Ala+Arg170Gly, Ala " refers to following variant:
" Tyr167Gly+Arg170Gly ", " Tyr167Gly+Arg170Ala ", " Tyr167Ala+Arg170Gly " and " Tyr167Ala+Arg170Ala ".
Parent's albumin
Albumin is albumen, and forms albumen the abundantest in blood plasma in Mammals, and characterizes by biochemical method and/or by sequence information from multiple mammiferous albumin.Several albumin, such as human serum albumin (HSA), is characterized by crystallographic methods, and its structure is determined.
HSA is the preferred albumin of the present invention, and for being made up of 585 amino-acid residues and there is the albumen of the molecular weight of 67kDa.At its natural form, it is without glycosylation.The aminoacid sequence of HSA is shown in SEQIDNO:2.It will be understood by those skilled in the art that to exist, with HSA, there is substantially the same characteristic, but there is the Natural allelic of one or more amino acid change compared with SEQIDNO:2, and the present inventor also contains this type of Natural allelic of use as parent's albumin of the present invention.
Albumin generally has the long plasma half-time of about 20 days or more of a specified duration, and such as, HSA has the plasma half-time of 19 days.The long plasma half-time of known HSA is by mediating with the interaction of its acceptor FcRn, but, for HSA long half life precise mechanism behind understanding or knowledge not essential to the present invention.
According to the present invention, term " albumin " means to have identical or very similar three-dimensional structure with HSA, and has the albumen of long plasma half-time.As albuminous example of the present invention, human serum albumin can be mentioned, primates serum albumin is (as chimpanzee serum albumin, gorilla serum albumin), rodents serum albumin is (as hamster sera albumin, guinea pig serum albumin, Mouse albumin and rat serum albumin), bovine serum albumin, horse serum albumin, donkey serum albumin, albumin rabbit serum, lowlenthal serum albumin, sheep serum albumin, dog serum albumin, chicken serum albumin and porcine hemoglobin.The allelotrope of HSA and any natural appearance thereof disclosed in SEQIDNO:2 is the preferred albumin of the present invention.
Parent's albumin of the present invention, its fragment, or the Albumin in Partial sequence that the is general and HSA shown in SEQIDNO:2 of the fusion polypeptide comprising albumin or its fragment has at least 60%, preferred at least 70%, preferred at least 80%, preferred at least 85%, preferred at least 86%, preferred at least 87%, preferred at least 88%, preferred at least 89%, preferred at least 90%, preferred at least 91%, preferred at least 92%, preferred at least 93%, preferred at least 94%, preferred at least 95%, more preferably at least 96%, more preferably at least 97%, more preferably at least 98%, and the most preferably sequence iden of at least 99%.
Described parent preferably comprises or consists of the aminoacid sequence of SEQIDNO:2.Described parent comprises or consists of the mature polypeptide of SEQIDNO:2 in yet another aspect.
In another embodiment, described parent is the allelic variant of the mature polypeptide of SEQIDNO:2.
In second, described parent is by polynucleotide encoding, described polynucleotide are at unusual low stringency condition, low stringency condition, medium stringency condition, in-Gao stringent condition, with following hybridization under high stringent condition or very high stringent condition: the mature polypeptide encoded sequence of (i) SEQIDNO:1; (ii) the mature polypeptide encoded sequence of SEQIDNO:1; Or total length complementary strand (J.Sambrook, E.F.Fritsch, the andT.Maniatis of (iii) (i) or (ii), 1989, MolecularCloning, ALaboratoryManual, 2dedition, ColdSpringHarbor, NewYork).
The polynucleotide of SEQIDNO:1 or its subsequence, and the aminoacid sequence of SEQIDNO:2 or its fragment, can be used for designing nucleic acid probe, with the DNA of the identification of strains never belonging to together according to method well known in the art and plant and clones coding parent.Specifically, according to the Southern immunoblot method of standard, these probes can be used for hybridizing with the genome of interested genus or kind or cDNA, with identify with from being wherein separated corresponding gene.These probes can be significantly shorter than complete sequence, but length should be at least 14, such as at least 25, at least 35, or at least 70 Nucleotide.Preferably, described nucleic acid probe is at least 100 length of nucleotides, such as, at least 200 Nucleotide, at least 300 Nucleotide, at least 400 Nucleotide, at least 500 Nucleotide, at least 600 Nucleotide, at least 700 Nucleotide, at least 800 Nucleotide, or the length of at least 900 Nucleotide.Both DNA and rna probe all can use.Usually probe mark (such as, is used to detect corresponding gene
32p,
3h,
35s, vitamin H or avidin (avidin) mark).This type of probe is contained in the present invention.
Can screen from the genomic dna prepared by these other organisms or cDNA library with above-mentioned probe hybridization and the DNA of the parent that encodes.Can agarose or polyacrylamide gel electrophoresis be passed through, or be separated from the genome of these other organisms or other DNA by other isolation technique.Can will be transferred to soluble cotton (nitrocellulose) or other suitable solid support material from the DNA in library or the DNA of separation and be fixed thereon.In order to identify and the clone of SEQIDNO:1 or its subsequence homology or DNA, described solid support material is used in Sounthern trace.
For the present invention, hybridization represents that polynucleotide hybridizes with the nucleotide probe of mark being low to moderate very much under very high stringent condition, described nucleotide probe corresponding to the polynucleotide shown in SEQIDNO:1, its complementary strand, or its subsequence.Such as X-ray sheet (X-rayfilm) or any other detection means as known in the art can be used to detect the molecule with described probe hybridization.
In one aspect, described nucleic acid probe is the mature polypeptide encoded sequence of SEQIDNO:1.In yet another aspect, described nucleic acid probe is the Nucleotide 1 to 1785 of SEQIDNO:1.In yet another aspect, described nucleic acid probe is the coding polypeptide of SEQIDNO:2 or the polynucleotide of its fragment.In yet another aspect, described nucleic acid probe is SEQIDNO:1.
For the long probe of length at least 100 Nucleotide, be defined as at 42 DEG C by being low to moderate very much very high stringent condition, to shear and in the salmon sperm DNA of sex change at 5XSSPE, 0.3%SDS, 200 μ g/ml, and for very low and low severity be 25% methane amide, be the methane amide of 35% for-high severity in neutralization or be the methane amide of 50% for high and very high severity, carry out prehybridization and best 12 to 24 hours of hybridization according to the Southern blotting of standard.Use 2XSSC, 0.2%SDS 45 DEG C (very low severity), 50 DEG C (low severity), 55 DEG C (middle severity), 60 DEG C (in-high severity), 65 DEG C (high severity), or solid support material is finally washed three times, each 15 minutes by 70 DEG C (very high severity).
For about 15 Nucleotide of length to the short probe of about 70 Nucleotide, stringent condition is defined as at the T calculated according to the computing method (1962, Proc.Natl.Acad.Sci.USA48:1390) of Bolton and McCarthy than use
mlow about 5 DEG C to about 10 DEG C, at 0.9MNaCl, 0.09MTris-HClpH7.6,6mMEDTA, 0.5%NP-40,1 × Denhardt solution, 1mM trisodium phosphate (sodiumpyrophosphate), 1mM SODIUM PHOSPHATE, MONOBASIC (sodiummonobasicphosphate), in the yeast rna of the every ml of 0.1mMATP and 0.2mg, carries out prehybridization according to the Southern blot procedure of standard and hybridizes best 12 to 24 hours.Described solid support material is added final in 0.1%SDS washing one time 15 minutes at 6 × SSC, and with 6 × SSC at the T than calculating
mthe temperature washes twice of low 5 DEG C to 10 DEG C, each 15 minutes.
In the third aspect, described parent has at least 60% by the mature polypeptide encoded sequence had with SEQIDNO:1, such as, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, the polynucleotide encoding of the sequence of at least 99% or 100% sequence iden, described polynucleotide encoding can play the polypeptide of albumin function.In one embodiment, described parent is by the polynucleotide encoding comprising or consist of SEQIDNO:1.
The preparation of variant
Further, the present invention relates to for the preparation of variant albumin, its fragment, or comprise the method for fusion polypeptide of variant albumin or its fragment, it comprises following step:
A. in albumin or its fragment, or comprise in the Albumin in Partial of fusion polypeptide of albumin or its fragment, identify for the important one or more amino acid residue positions of albumin bound FcRn;
B. provide coding described albumin, its fragment, or comprise the nucleic acid of Albumin in Partial of fusion polypeptide of albumin or its fragment;
C., the nucleic acid provided in b is provided, one or more (several) amino-acid residue being located at the position identified in a is lacked or replaces with different aminoacids or insert.
D. in suitable host cell, express the nucleic acid of modification; With
E. described variant albumin is reclaimed, its fragment or comprise the fusion polypeptide of variant albumin or its fragment.
In albumin, its fragment, or in the Albumin in Partial of fusion polypeptide, identify that the one or more amino acid residue positions for albumin bound FcRn is important can carry out with several means, include but not limited to that random mutagenesis continues to analyze the mutant of generation, and compare with the parent molecules do not suddenlyd change, and the qualification that structure based is considered, optionally continue to generate the variant with the change identified, and compared with the parent molecules do not suddenlyd change.
For the identification of one or more amino acid residue positions to be changed, to prepare to have compared with natural HSA, the preferred method of the variant HSA that the change of FcRn combines is comprised the steps:
I) the non-human albumin had the different binding characteristic of FcRn is identified;
Ii) identifier's serum albumin and the interactional amino-acid residue of FcRn;
Iii) for step I i) in the one-level of the non-human albumin that goes out of the amino-acid residue Identification that identifies and human serum albumin and/or tertiary structure, and different amino-acid residue between described non-human albumin and human serum albumin is accredited as cause viewed in conjunction with difference; With
Iv) optionally, preparation HSA is at step I ii) in the variant of position of qualification, and confirm that the variant of preparation has the combination to FcRn of change compared with HSA.
Above-mentioned steps i) hereinafter described SPR assay method can be used to implement.But, it will be understood by those skilled in the art that additive method can be used for identifying to have the non-human albumin of the different binding characteristics to FcRn compared with HSA, and how described method the non-human albumin described in not relying on the different binding characteristics to FcRn identify.
In a preferred embodiment, the non-human albumin identified, compared with HSA, has the stronger combination to FcRn.The inhuman albuminous example compared with HSA with the stronger combination to FcRn comprises donkey serum albumin, albumin rabbit serum, dog serum albumin, hamster sera albumin, guinea pig serum albumin, mice serum albumin and rat serum albumin.Step I i) by considering that FcRn, HSA and the structure in conjunction with mixture between the two realize.When lack obtainable in conjunction with composite structure, the model of HSA Structure anchor in the structure of FcRn structure can be used, and to identify in HSA the interactional amino-acid residue with FcRn thus.
In a further preferred embodiment, the non-human albumin identified has the more weak combination to FcRn compared with HSA.The inhuman albuminous example compared with HSA with the more weak combination to FcRn comprises bovine serum albumin, lowlenthal serum albumin, sheep serum albumin and chicken serum albumin.Step I i) by considering that FcRn, HSA and the structure in conjunction with mixture between the two realize.When lack obtainable in conjunction with composite structure, the model of HSA Structure anchor in the structure of FcRn structure can be used, and to identify in HSA the interactional amino-acid residue with FcRn thus.
In the present invention and claims, in HSA, interactional amino-acid residue is considered as any certain amino acid being arranged in FcRn in HSA with FcRn
within amino-acid residue, or anyly to relate to and certain amino acid being arranged in FcRn
within the hydrogen bond of amino-acid residue, salt bridge or polarity or apolar interaction amino-acid residue.Preferably, the amino acid in HSA residue is arranged in the amino acid whose of FcRn
within, amino acid whose more preferably in FcRn
within, and amino acid whose most preferably in FcRn
within.
Step I ii) and iv) can use and well known to a person skilled in the art technology implementation.
The invention still further relates to and obtain variant albumin or its fragment, or comprise the fusion polypeptide of described variant albumin or its fragment, or the method for the associated complex of variant albumin or its fragment, comprise: (a) is by the position 417 at the mature polypeptide corresponding to SEQIDNO:2, 440, 464, 490, 492, 493, 494, 495, 496, 499, 500, 501, 503, 504, 505, 506, 510, 535, 536, 537, 538, 540, 541, 542, 550, 573, 574, 575, 577, 578, 579, 580, 581, the change of one or more (several) position of 582 and 584 imports parent's albumin or its fragment, or comprise the fusion polypeptide of described parent's albumin or its fragment, (b) reclaim described variant albumin or its fragment, or comprise the fusion polypeptide of described variant albumin or its fragment.
Described variant can use any known mutagenesis steps in this area by those skilled in the art, and as site-directed mutagenesis, synthetic gene builds, semi-synthetic gene constructed, random mutagenesis, prepared by reorganization etc.
Site-directed mutagenesis is that one or more in the polynucleotide of coding parent determine that site creates one or more (several) technology of suddenling change.
Site-directed mutagenesis can be reached by PCR in vitro, relates to the Oligonucleolide primers used containing desired sudden change.Site-directed mutagenesis also can be undertaken by expression cassette mutagenesis in vitro, relates to and cutting with the site of restriction enzyme in the plasmid of polynucleotide comprising coding parent, be then connected in described polynucleotide by the oligonucleotide containing sudden change.Usually the restriction enzyme carrying out digesting on plasmid and on oligonucleotide is identical, and described plasmid and inset are connected to each other.See, such as, Scherer and Davis, 1979, Proc.Natl.Acad.Sci.USA76:4949-4955; And Barton etc., 1990, NucleicAcidsResearch18:7349-4966.
Site-directed mutagenesis also can be reached by means known in the art in vivo.See, such as, U.S. Patent Application Publication No. 2004/0171154; Storici etc., 2001, NatureBiotechnol.19:773-776; Kren etc., 1998, Nat.Med.4:285-290; And Calissano and Macino, 1996, FungalGenet.Newslett.43:15-16.
Any site-directed mutagenesis method all can be used for the present invention.Many available commercial reagents boxes are had to can be used for preparing variant.
It is the polynucleotide molecule of encoding target polypeptide that synthetic gene structure relates to compounding design in vitro.Gene chemical synthesis can use multiple technologies to carry out, as by Tian etc. (2004, Nature432:1050-1054) the described technology based on multichannel microchip (multiplexmicrochip-based), and similar technology, wherein synthetic oligonucleotide in the upper assembling of the micro flow chip (microfluidicchip) of available light programming (photo-programmable).
Known mutagenesis, restructuring and/or Shuffling Method can be used, then carry out the screening process of being correlated with, as by Reidhaar-Olson and Sauer, 1988, Science241:53-57; Bowie and Sauer, 1989, Proc.Natl.Acad.Sci.USA86:2152-2156; WO95/17413; Or those disclosed in WO95/22625, carry out one or more aminoacid replacement, disappearance and/or insert and tested.Other spendable methods comprise fallibility PCR, phage display (such as Lowman etc., 1991, Biochemistry.30:10832-10837; U.S. Patent number 5,223,409; And regiondirected mutagenesis (region-directedmutagenesis) (Derbyshire etc., 1986, Gene46:145 WO92/06204); Ner etc., 1988, DNA7:127).
Mutagenesis/Shuffling Method can with high-throughput, auto-screening method combine with detect by host cell expression through cloning, the activity (Ness etc., 1999, NatureBiotechnology17:893-896) of the polypeptide of mutagenesis.The DNA molecular through mutagenesis of encode active polypeptides can reclaim from host cell and use this area standard method to check order rapidly.These methods make to determine that the importance of single amino acids residue in polypeptide becomes possibility fast.
Semi-synthetic gene constructed gene constructed by combinatorial compound and/or site-directed mutagenesis and/or random mutagenesis and/or reorganization feature is reached.Semi-synthetic structure is generally the method using the polynucleotide passage of synthesis and round pcr to combine.Thus the determination region of gene can start anew to synthesize, and other regions can use site-directed mutagenesis primer amplification, also can carry out fallibility PCR or non-fallibility PCR (non-errorpronePCR) amplification to other other regions.Then polynucleotide subfragment can be reorganized.
Variant
Present invention also offers the albuminous variant albumin of parent or its fragment, or comprise the fusion polypeptide of described variant albumin or its fragment, it is corresponding to position 417 in SEQIDNO:2, 440, 464, 490, 492, 493, 494, 495, 496, 499, 500, 501, 503, 504, 505, 506, 510, 535, 536, 537, 538, 540, 541, 542, 550, 573, 574, 575, 577, 578, 579, 580, 581, one or more (several) position of 582 and 584 comprises change, wherein each change is independently for replacing, insert or disappearance, its prerequisite is that described variant not has replacement D494N, E501K, K541E, D550G, A, the SEQIDNO:2 of K573E or K574N.
Variant albumin of the present invention, its fragment, or the Albumin in Partial of the fusion polypeptide comprising variant albumin or its fragment usually has at least 60% with the sequence of the HSA shown in SEQIDNO:2, preferably at least 70%, preferred at least 80%, preferably at least 85%, preferably at least 90%, more preferably at least 95%, more preferably at least 96%, more preferably at least 97%, more preferably at least 98%, and most preferably at least 99% sequence iden.
In one aspect, the change number in variant of the present invention is 1-20, such as 1-10 and 1-5, as 1,2,3,4,5,6,7,8,9 or 10 change.
Described variant albumin, its fragment, or comprise the fusion polypeptide of described variant albumin or its fragment and corresponding parent's albumin, its fragment, or the fusion polypeptide comprising described variant albumin or its fragment compares the plasma half-time with change.
In an especially preferred embodiment, described parent's albumin is HSA, and described variant albumin, its fragment, or comprise fusion polypeptide and the HSA of described variant albumin or its fragment, the fragment of its correspondence, or the fusion polypeptide comprising HSA or its fragment compares the plasma half-time with change.
Based on the allelotrope HSAD494N of natural appearance, the present inventor recognizes that albumin is to the combination of its acceptor and associating between plasma half-time.Contriver analyzes this allelotrope, and finds that it has lower avidity to its acceptor FcRn.
In addition, disclosed the transgenic mice that its native mouse FcRn employment FcRn substitutes and there is higher serum albumin levels compared with normal mouse, see JExpMed. (2003) 197 (3): 315-22.The present inventor finder FcRn to the albuminous avidity of mice serum and mouse FcRn to higher compared with the albuminous avidity of mice serum, therefore, the sero-abluminous increase observed in transgenic mice is consistent with higher affinity between serum albumin and its acceptor, confirms albumin to the combination of FcRn and associating between plasma half-time.In addition, shown in mouse model to FcRn almost without or without the albuminous variant combined, there is the half life of minimizing, Kenanova etc. (2009) J.Nucl.Med.; 50 (Supplement2): 1582).
A kind ofly determine that whether variant albumin is use surface plasma resonance as described below to measure (SurfacePlasmonResonanceassay, SPR) higher or lower than the albuminous mode of parent to the avidity of FcRn.It will be understood by those skilled in the art that and whether can use additive method determination variant albumin to the avidity of FcRn higher or lower than the avidity of parent's albumin to FcRn, such as, determine and compare binding constant KD.Therefore, the variant albumin according to the present invention with the KD of the KD lower than natural HSA is considered as having plasma half-time higher compared with HSA, and the variant albumin with the KD of the KD higher than natural HSA is considered as having plasma half-time lower compared with HSA.
Albuminous variant or its fragment, or comprise the fusion polypeptide of albumin or its fragment, one or more change is being comprised, as replaced, lack or inserting: 417,440,464,490,492,493,494,495,496,499,500,501,503,504,505,506,510,535,536,537,538,540,541,542,550,573,574,575,577,578,579,580,581,582 and 584 corresponding to one or more (several) position being selected from the position of lower group in HSA.Described replacement can be any replacement, the amino acid wherein in native albumin sequence by be selected from other in 19 the amino acid whose different aminoacids of natural appearance replaced.
In one aspect, one or more (several) position that variant is corresponding to the position 417,440,464,490,492,493,494,495,496,499,500,501,503,504,505,506,510,535,536,537,538,540,541,542,550,573,574,575,577,578,579,580,581,582 and 584 in SEQIDNO:2 comprises change.In yet another aspect, two positions that variant is corresponding in SEQIDNO:2 417,440,464,490,492,493,494,495,496,499,500,501,503,504,505,506,510,535,536,537,538,540,541,542,550,573,574,575,577,578,579,580,581,582 and 584 comprise change.In yet another aspect, three positions that variant is corresponding to the position 417,440,464,490,492,493,494,495,496,499,500,501,503,504,505,506,510,535,536,537,538,540,541,542,550,573,574,575,577,578,579,580,581,582 and 584 in SEQIDNO:2 comprise change.In yet another aspect, each position that variant is corresponding to the position 417,440,464,490,492,493,494,495,496,499,500,501,503,504,505,506,510,535,536,537,538,540,541,542,550,573,574,575,577,578,579,580,581,582 and 584 in SEQIDNO:2 comprises change.
In yet another aspect, described variant comprises the replacement Q417A of the mature polypeptide of SEQIDNO:2, H.In yet another aspect, described variant comprises the replacement H440Q of the mature polypeptide of SEQIDNO:2.In yet another aspect, described variant comprises the replacement H464Q of the mature polypeptide of SEQIDNO:2.In yet another aspect, described variant comprises the replacement A490D of the mature polypeptide of SEQIDNO:2.In yet another aspect, described variant comprises the replacement E492G of the mature polypeptide of SEQIDNO:2, T, P, H.In yet another aspect, described variant comprises the replacement V493P of the mature polypeptide of SEQIDNO:2, L.In yet another aspect, described variant comprises the replacement D494N of the mature polypeptide of SEQIDNO:2, Q, A, E, P.In yet another aspect, described variant comprises the replacement E495Q of the mature polypeptide of SEQIDNO:2, A.In yet another aspect, described variant comprises the replacement T496A of the mature polypeptide of SEQIDNO:2.In yet another aspect, described variant comprises the replacement P499A of the mature polypeptide of SEQIDNO:2.In yet another aspect, described variant comprises the replacement K500E of the mature polypeptide of SEQIDNO:2, G, D, A, S, C, P, H, F, N, W, T, M, Y, V, Q, L, I, R.In yet another aspect, described variant comprises the replacement E501A of the mature polypeptide of SEQIDNO:2, P, Q.In yet another aspect, described variant comprises the replacement N503K of the mature polypeptide of SEQIDNO:2, D, H.In yet another aspect, described variant comprises the replacement A504E of the mature polypeptide of SEQIDNO:2.In yet another aspect, described variant comprises the replacement E505K of the mature polypeptide of SEQIDNO:2, D.In yet another aspect, described variant comprises the replacement T506F of the mature polypeptide of SEQIDNO:2, S.In yet another aspect, described variant comprises the replacement H510Q of the mature polypeptide of SEQIDNO:2.In yet another aspect, described variant comprises the replacement H535Q of the mature polypeptide of SEQIDNO:2.In yet another aspect, described variant comprises the replacement K536A of the mature polypeptide of SEQIDNO:2.In yet another aspect, described variant comprises the replacement P537A of the mature polypeptide of SEQIDNO:2.In yet another aspect, described variant comprises the replacement K538A of the mature polypeptide of SEQIDNO:2, H.In yet another aspect, described variant comprises the replacement T540S of the mature polypeptide of SEQIDNO:2.In yet another aspect, described variant comprises the replacement K541A of the mature polypeptide of SEQIDNO:2, D, G, N, E.In yet another aspect, described variant comprises the replacement E542P of the mature polypeptide of SEQIDNO:2, D.In yet another aspect, described variant comprises the replacement D550N of the mature polypeptide of SEQIDNO:2.In yet another aspect, described variant comprises the replacement K573Y of the mature polypeptide of SEQIDNO:2, W, P, H, F, V, I, T, N, S, G, M, C, A, E, Q, R, L, D.In yet another aspect, described variant comprises the replacement K574N of the mature polypeptide of SEQIDNO:2.In yet another aspect, described variant comprises the replacement Q580K of the mature polypeptide of SEQIDNO:2.In yet another aspect, described variant comprises the replacement L575F of the mature polypeptide of SEQIDNO:2.In yet another aspect, described variant comprises the replacement A577T of the mature polypeptide of SEQIDNO:2, E.In yet another aspect, described variant comprises the replacement A578R of the mature polypeptide of SEQIDNO:2, S.In yet another aspect, described variant comprises the replacement S579C of the mature polypeptide of SEQIDNO:2, T.In yet another aspect, described variant comprises the replacement Q580K of the mature polypeptide of SEQIDNO:2.In yet another aspect, described variant comprises the replacement A581D of the mature polypeptide of SEQIDNO:2.In yet another aspect, described variant comprises the replacement A582T of the mature polypeptide of SEQIDNO:2.In yet another aspect, described variant comprises the replacement G584A of the mature polypeptide of SEQIDNO:2.
In one aspect, the position that described variant is corresponding to position 417 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 417, or Val, preferred Ala or His.In yet another aspect, described variant comprises the replacement Q417A of the mature polypeptide of SEQIDNO:2, H.
In yet another aspect, the position that described variant is corresponding to position 440 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 440, or Val, preferred Ala.In yet another aspect, described variant comprises the replacement H440Q of the mature polypeptide of SEQIDNO:2.
In yet another aspect, the position that described variant is corresponding to position 464 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 464, or Val, preferred Ala.In yet another aspect, described variant comprises the replacement H464Q of the mature polypeptide of SEQIDNO:2.
In yet another aspect, the position that described variant is corresponding to position 490 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 490, or Val.In yet another aspect, described variant comprises the replacement A490G of the mature polypeptide of SEQIDNO:2.
In yet another aspect, the position that described variant is corresponding to position 492 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 492, or Val, preferred Gly.In yet another aspect, described variant comprises the replacement E492G of the mature polypeptide of SEQIDNO:2.
In yet another aspect, the position that described variant is corresponding to position 493 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 493, or Val, preferred Pro.In yet another aspect, described variant comprises the replacement V493P of the mature polypeptide of SEQIDNO:2.
In yet another aspect, the position that described variant is corresponding to position 494 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 494, or Val, preferred Asn, Gln or Ala.In yet another aspect, described variant comprises the replacement D494N of the mature polypeptide of SEQIDNO:2, Q, A.
In yet another aspect, the position that described variant is corresponding to position 495 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 495, or Val, preferred Gln or Ala.In yet another aspect, described variant comprises replacement E495Q or A of the mature polypeptide of SEQIDNO:2.
In yet another aspect, the position that described variant is corresponding to position 496 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 496, or Val, preferred Ala.In yet another aspect, described variant comprises the replacement T496A of the mature polypeptide of SEQIDNO:2.
In yet another aspect, the position that described variant is corresponding to position 499 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 499, or Val, preferred Ala.In yet another aspect, described variant comprises the replacement P499A of the mature polypeptide of SEQIDNO:2.
In yet another aspect, the position that described variant is corresponding to position 500 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 500, or Val, preferred Ala.In yet another aspect, described variant comprises the replacement K500E of the mature polypeptide of SEQIDNO:2, G, D, A, S, C, P, H, F, N, W, T, M, Y, V, Q, L, I, R.
In yet another aspect, the position that described variant is corresponding to position 501 comprises change.In yet another aspect, be Ala, Arg, Asn at the aminoacid replacement of position corresponding to position 501, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferred Ala or Gln (to reduce avidity) and Pro (to increase avidity).In yet another aspect, described variant comprises the E501A of the mature polypeptide replacing SEQIDNO:2, Q, P.
In yet another aspect, the position that described variant is corresponding to position 503 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 503, or Val, preferred Asp or Lys or His.In yet another aspect, described variant comprises the replacement N503D of the mature polypeptide of SEQIDNO:2, K, H.
In yet another aspect, the position that described variant is corresponding to position 504 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 504, or Val.In yet another aspect, described variant comprises the replacement A504 of the mature polypeptide of SEQIDNO:2.
In yet another aspect, the position that described variant is corresponding to position 505 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 505, or Val.In yet another aspect, described variant comprises the replacement E505D of the mature polypeptide of SEQIDNO:2.
In yet another aspect, the position that described variant is corresponding to position 506 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 506, or Val.In yet another aspect, described variant comprises the replacement T506S of the mature polypeptide of SEQIDNO:2, F.
In yet another aspect, the position that described variant is corresponding to position 510 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 510, or Val, preferred Gln.In yet another aspect, described variant comprises the replacement H510Q of the mature polypeptide of SEQIDNO:2.
In yet another aspect, the position that described variant is corresponding to position 535 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 535, or Val, preferred Gln.In yet another aspect, described variant comprises the replacement H535Q of the mature polypeptide of SEQIDNO:2.
In yet another aspect, the position that described variant is corresponding to position 536 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 536, or Val, preferred Ala.In yet another aspect, described variant comprises the replacement K536A of the mature polypeptide of SEQIDNO:2.
In yet another aspect, the position that described variant is corresponding to position 537 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 537, or Val, preferred Ala.In yet another aspect, described variant comprises the replacement P537A of the mature polypeptide of SEQIDNO:2.
In yet another aspect, the position that described variant is corresponding to position 538 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 538, or Val, preferred Ala.In yet another aspect, described variant comprises the replacement K538H of the mature polypeptide of SEQIDNO:2, A.
In yet another aspect, the position that described variant is corresponding to position 540 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 540, or Val.In yet another aspect, described variant comprises the replacement T540S of the mature polypeptide of SEQIDNO:2.
In yet another aspect, the position that described variant is corresponding to position 541 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 541, or Val, preferred Gly, Asp or Ala.In yet another aspect, described variant comprises the replacement K541G of the mature polypeptide of SEQIDNO:2, DA, N.
In yet another aspect, the position that described variant is corresponding to position 542 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 542, or Val, preferred Asp or Pro.In yet another aspect, described variant comprises the E542D of the mature polypeptide replacing SEQIDNO:2, P.
In yet another aspect, the position that described variant is corresponding to position 550 comprises change.In yet another aspect, be Ala, Arg, Asn at the aminoacid replacement of position corresponding to position 550, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferred Asn (to reduce avidity), preferred Glu (to increase avidity).
In yet another aspect, the position that described variant is corresponding to position 573 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 573, or Val, preferred Tyr, Trp, Pro, His.Phe，Val，Ile，Thr，Asn，Ser，Gly，Met，Cys，Ala，Glu，Gln，Arg，Leu，Asp。In yet another aspect, described variant comprises the replacement K573Y of the mature polypeptide of SEQIDNO:2, W, P, H, F, V, I, T, N, S, G, M, C, A, E, Q, R, L, D.
In yet another aspect, the position that described variant is corresponding to position 574 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 574, or Val, preferred Asn.In yet another aspect, described variant comprises the replacement K574N of the mature polypeptide of SEQIDNO:2.
In yet another aspect, the position that described variant is corresponding to position 575 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 575, or Val, preferred Phe.In yet another aspect, described variant comprises the replacement L575F of the mature polypeptide of SEQIDNO:2.
In yet another aspect, the position that described variant is corresponding to position 577 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 577, or Val, preferred Thr or Glu.In yet another aspect, described variant comprises the replacement A577TE of the mature polypeptide of SEQIDNO:2.
In yet another aspect, the position that described variant is corresponding to position 578 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 578, or Val, preferred Arg or Ser.In yet another aspect, described variant comprises the replacement A578R of the mature polypeptide of SEQIDNO:2, S.
In yet another aspect, the position that described variant is corresponding to position 579 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 579, or Val, preferred Cys or Thr.In yet another aspect, described variant comprises the replacement S579C of the mature polypeptide of SEQIDNO:2, T.
In yet another aspect, the position that described variant is corresponding to position 580 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 580, or Val, preferred Lys.In yet another aspect, described variant comprises the replacement Q580K of the mature polypeptide of SEQIDNO:2.
In yet another aspect, the position that described variant is corresponding to position 581 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 581, or Val, preferred Asp.In yet another aspect, described variant comprises the replacement A581D of the mature polypeptide of SEQIDNO:2.
In yet another aspect, the position that described variant is corresponding to position 582 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 582, or Val, preferred Thr.In yet another aspect, described variant comprises the replacement A582T of the mature polypeptide of SEQIDNO:2.
In yet another aspect, the position that described variant is corresponding to position 584 comprises change.In yet another aspect, be Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr at the aminoacid replacement of the position corresponding to position 584, or Val, preferred Ala.In yet another aspect, described variant comprises the replacement G584A of the mature polypeptide of SEQIDNO:2.
In yet another aspect, described variant is comprising change corresponding to the position of position 494 and 496 in SEQIDNO:2, as above-mentioned those.
In yet another aspect, described variant is comprising change corresponding to the position of position 492 and 493 in SEQIDNO:2, as above-mentioned those.
In yet another aspect, described variant is comprising change corresponding to the position of position 494 and 417 in SEQIDNO:2, as above-mentioned those.
In yet another aspect, described variant is comprising change corresponding to the position of position 492 and 503 in SEQIDNO:2, as above-mentioned those.
In yet another aspect, described variant is comprising change corresponding to the position of position 492 and 573 in SEQIDNO:2, as above-mentioned those.
In yet another aspect, described variant is comprising change corresponding to the position of position 492,503 and 573 in SEQIDNO:2, as above-mentioned those.
In one embodiment, variant albumin of the present invention or its fragment, or comprise the fusion polypeptide of described variant albumin or its fragment, replacement: 417 in a SEQIDNO:2 is being contained corresponding to the position being selected from the position of lower group in HSA, 440, 464, 490, 492, 493, 494, 495, 496, 499, 500, 501, 503, 504, 505, 506, 510, 535, 536, 537, 538, 540, 541, 542, 550, 573, 574, 575, 577, 578, 579, 580, 581, 582 and 584, prerequisite is that described variant albumin is not by having replacement D494N, E501K, K541E, D550G, A, the variant of the SEQIDNO:2 composition of K573E or K574N.Variant albumin of the present invention, its fragment, or the fusion polypeptide comprising variant albumin or its fragment can comprise other replacements, insert or disappearance in one or more (several) position corresponding to other positions in HSA.
In another embodiment, variant albumin of the present invention or its fragment, or comprise the fusion polypeptide of described variant albumin or its fragment, two are being contained corresponding to the position being selected from the position of lower group in HSA, three, four, five, six, seven, eight, nine, ten, 11, 12, 13, 14, 15, 16, 17, 18, nineteen, 20 or even more replacement: 417 of SEQIDNO:2, 440, 464, 490, 492, 493, 494, 495, 496, 499, 500, 501, 503, 504, 505, 506, 510, 535, 536, 537, 538, 540, 541, 542, 550, 573, 574, 575, 577, 578, 579, 580, 581, 582 and 584.Variant albumin of the present invention or its fragment, or the fusion polypeptide comprising variant albumin or its fragment can comprise other replacements, insert or disappearance in the position corresponding to other positions in HSA.
In further embodiment, variant albumin of the present invention or its fragment, or comprise the fusion polypeptide of described variant albumin or its fragment, with parent's albumin, its fragment, or the plasma half-time comprising the fusion polypeptide of described parent's albumin or its fragment is compared, there is longer plasma half-time.Comprise variant albumin or its fragment of replacement according to the example of this embodiment position be included in corresponding to 492,503,542,550,573,574,580,581,582 or 584 in SEQIDNO:2, or comprise the fusion polypeptide of variant albumin or its fragment.According to the preferred replacement of the embodiment of the present invention be included in corresponding in SEQIDNO:2 492 the replacement with G residue of amino-acid residue of position, corresponding to the replacement with H or K residue of amino-acid residue of position of in SEQIDNO:2 503, corresponding to the replacement with E residue of amino-acid residue of position of in SEQIDNO:2 550, correspond in SEQIDNO:2 573 position amino-acid residue with Y, W, P, H, F, V, I, T, N, S, G, M, C, A, E, Q, R, the replacement of L or D, corresponding to the replacement with N residue of amino-acid residue of position of in SEQIDNO:2 574, or corresponding to the replacement with K residue of amino-acid residue of position of in SEQIDNO:2 580.Other preferred variants have the replacement at the position G residue corresponding in SEQIDNO:2 492, and have and corresponding to the position A of 573 or the replacement of P residue in SEQIDNO:2.Other preferred variants have in the multiple replacement in position corresponding to position 492 in SEQIDNO:2, and the position of 503 is H residue in SEQIDNO:2.
Other preferred variants have the replacement at the position G residue corresponding in SEQIDNO:2 492, and correspond to the replacement of H or K in the position corresponding to position 503 in SEQIDNO:2, and position 573 replacement of A or P residue in SEQIDNO:2.
In further embodiment, variant albumin of the present invention or its fragment, or comprise the fusion polypeptide of described variant albumin or its fragment, with parent's albumin, its fragment, or the fusion polypeptide comprising described parent's albumin or its fragment is compared, and has shorter plasma half-time.Be included in corresponding to 417 in SEQIDNO:2 according to the example of this embodiment, 440,494,495,496,499,500,501,536,537,538, the position of 541,494+496 or 492+493 comprises variant albumin or its fragment of replacement, or comprises the fusion polypeptide of variant albumin or its fragment.Preferred replacement comprises corresponding to Q417A, H440Q, D494E+Q417H, D494N, Q, A in SEQIDNO:2, E495Q, A, T496A, D494N+T496A or P499A, K500A, E501A, the replacement of E501Q, K536A, P537A, K538A, K541G, K541AK541D or D550N.
In another embodiment of the invention, variant albumin of the present invention or its fragment, or the fusion polypeptide comprising described variant albumin or its fragment loses its ability in conjunction with FcRn.In this association, if the resonance units recorded variant in SPR as described below measures is less than 10% of the resonance units that parent's albumin or its fragment for correspondence record, then albumin variants or its fragment, or the fusion polypeptide comprising albumin variants or its fragment is regarded as losing the ability in conjunction with FcRn.According to the example of this embodiment be included in corresponding in SEQIDNO:2 464,500,510 or 535 position comprise variant albumin or its fragment of replacement, or comprise the fusion polypeptide of described variant albumin or its fragment.Preferred replacement comprises the replacement corresponding to H464Q, K500A, P, C, S, A, D.GH510Q or H535Q in SEQIDNO:2.
Except corresponding to position 417,464,490,492,493,494 in SEQIDNO:2,495,496,499,500,501,503,504,505,506,510,535,536,537,538,540,541,542,550,573,574, outside one or more replacements of one or more positions of 580,581,582 and 584, variant albumin of the present invention or its fragment, or the fusion polypeptide comprising described variant albumin or its fragment can contain in other positions of molecule that other replace, disappearance or insert.These type of other replacement, disappearance or insertion can be used for other characteristics changing molecule, as but be not limited to change glycosylation, import surface reaction group as thiol group, remove/generate carbamylation site etc.
The reactive residue provided on surface can be provided, and advantageously can be applied to residue of the present invention and be disclosed in undocumented patent application WO2010/092135 (being incorporated to by carrying stating).Particularly preferred residue comprises the position corresponding to the position in SEQIDNO:2.
As carrying out providing the example of the change of reactive thiol group to comprise change corresponding to changing in following SEQIDNO:2 on surface by correspondence position in SEQIDNO:2 or in other albumin: L585C, D1C, A2C, D562C, A364C, A504C, E505C, T79C, E86C, D129C, D549C, A581C, D121C, E82C, S270C, A578C, L595LC, D1DC, A2AC, D562DC, A364AC, A504AC, E505EC, T79TC, E86EC, D129DC, D549DC, A581AC, A581AC, D121DC, E82EC, S270SC, A579AC, C360*, C316*, C75*, C168*, C558*, C361*, C91*, C124*, C169* and C567*.Or, cysteine residues can be added at albuminous N or C end.
Polynucleotide
The invention still further relates to the polynucleotide that code book invents the separation of any variant.
Nucleic acid construct
The invention still further relates to the nucleic acid construct of the polynucleotide comprising variant of the present invention of encoding, described polynucleotide are operably connected with one or more (several) regulating and controlling sequence, and described regulating and controlling sequence instructs the expression of encoding sequence in suitable host cell under the condition compatible with this regulating and controlling sequence.
Can with being permitted multi-mode operation polynucleotide to provide the expression of variant.Depend on expression vector, it may be desirable or required for operating on it before by polynucleotide insertion vector.The technology using recombinant DNA method to modify polynucleotide is well known in the art.
Regulating and controlling sequence can be promoter sequence, its by host cell identification for expression polynucleotide.Promoter sequence contains the transcription regulating nucleotide sequence of the expression of mediation variant.Promotor can be any nucleotide sequence showing transcriptional activity in host cell, comprise sudden change, brachymemma with the promotor of heterozygosis, and can to obtain by the gene of polypeptide from the born of the same parents of coding and host cell homology or allos or in born of the same parents.
In yeast host, useful promotor obtains from the gene of following enzyme: yeast saccharomyces cerevisiae (Saccharomycescerevisiae) Hydratase, phosphoenolpyruvate (ENO-1), Saccharomyces cerevisiae protein enzyme A (PRA1), Saccharomyces cerevisiae protein enzyme B (PRB1), yeast saccharomyces cerevisiae translation elongation factor (TEF1), yeast saccharomyces cerevisiae translation elongation factor (TEF2), yeast saccharomyces cerevisiae galactokinase (GAL1), yeast saccharomyces cerevisiae alcoholdehydrogenase/glyceraldehyde-3-phosphate dehydrogenase (ADH1, ADH2/GAP), yeast saccharomyces cerevisiae triose-phosphate isomerase (TPI), brewing yeast metallothionein (CUP1) and yeast saccharomyces cerevisiae glycerol 3-phosphate acid kinase.For useful other promotor of yeast host cell by Romanos etc., 1992, Yeast8:423-488 describes.
Regulating and controlling sequence also can be suitable transcription terminator sequences, and it is transcribed with termination by host cell identification.Described terminator sequence is operably connected with 3 ' end of the polynucleotide of the described variant of coding.Any terminator having function can be used in host cell.
The preferred terminator of yeast host cell is obtained from the gene of following enzyme: yeast saccharomyces cerevisiae Hydratase, phosphoenolpyruvate, S. cerevisiae cytochrome C (CYC1), yeast saccharomyces cerevisiae alcoholdehydrogenase (ADH1) and S. cerevisiae glyceraldehyde-3-phosphate dehydrogenase.For useful other terminator of yeast host cell by Romanos etc., 1992, see upper description.
Regulating and controlling sequence can also be suitable leader sequence, and it is for the important mRNA non-translational region of the translation of host cell.Leader sequence is operably connected to 5 ' end of the polynucleotide of encode variant.Any leader sequence having function can be used in host cell.
The leader sequence suitable for yeast host cell obtains from the gene of following enzyme: yeast saccharomyces cerevisiae Hydratase, phosphoenolpyruvate (ENO-1), yeast saccharomyces cerevisiae glycerol 3-phosphate acid kinase, cerevisiae alpha-factor and yeast saccharomyces cerevisiae alcoholdehydrogenase/glyceraldehyde-3-phosphate dehydrogenase (ADH2/GAP).
Regulating and controlling sequence also can be polyadenylation se-quence, and it is the sequence be operably connected with 3 ' end of variant coding sequences, and when transcribing, host cell is identified as the signal adding poly-adenosine residue to the mRNA transcribed.Any polyadenylation se-quence having function can be used in host cell.
For the useful polyadenylation se-quence of yeast host cell by Guo and Sherman, 1995, Mol.CellularBiol.15:5983-5990 describes.
Regulating and controlling sequence can also be signal peptide coding region, and its coding holds with the N of variant the signal peptide be connected, and instructs variant to enter emiocytosis approach.Encoding sequence 5 ' the end of polynucleotide can comprise signal peptide coding region inherently, and this signal peptide coding region is connected to natively and translates in reading frame together with the section of the coding region of encode variant.Or it is the signal peptide coding region of external source that encoding sequence 5 ' end can contain for described coding region.Exogenous signals peptide-coding region is unrequired containing can be during signal peptide coding region natively at encoding sequence.Or exogenous signals peptide-coding region can replace natural signals peptide-coding region simply to strengthen the secretion of variant.But, any signal peptide coding region of the Secretory Pathway of host cell can be entered by the instruction variant of expressing.
The signal peptide useful for yeast host cell obtains from the gene of cerevisiae alpha-factor and Saccharomyces cerevisiae invertase.Other useful signal coding sequence is described in Romanos etc., 1992, on seeing.
When both signal peptide and propetide district all appear at the N end of variant, make the position in propetide district and then (nextto) variant N hold, and the N in and then propetide district, the position making signal peptide district holds.
Production method
Variant of the present invention can use and well known to a person skilled in the art prepared by technology.Method is by clones coding parent albumin or its fragment or the nucleic acid of fusion polypeptide comprising albumin or its fragment easily, modifies described nucleic acid to correspond to position 417 in SEQIDNO:2, 464, 490, 492, 493, 494, 495, 496, 499, 500, 501, 503, 504, 505, 506, 510, 535, 536, 537, 538, 540, 541, 542, 550, 573, the replacement needed for the importing of one or more (several) position of 574 and 580, wherein said variant is not by having replacement D494N, E501K, K541E, D550G, A, the variant of the SEQIDNO:2 composition of K573E or K574N, prepare suitable gene construct, wherein modified nucleic acid and suitable adjustment genetic elements are as promotor, terminator, activate site, ribosome bind sites etc. are operably connected, and described gene construct is imported suitable host living beings, cultivate the host living beings through transforming, and reclaim described variant under the condition causing described variant to be expressed.All these technology are well known in the art, and those skilled in the art have the ability of the suitable method of design for preparation concrete variant of the present invention.
Also variant polypeptide of the present invention can be connected to signal sequence and in the host living beings culturing process through transforming, be secreted into growth medium to make described variant polypeptide.Variant polypeptide is made to be secreted into growth medium so that recovery and purifying are generally favourable.
Technology for the preparation of variant polypeptide is also disclosed in WO2009019314 (being incorporated to by carrying stating), and these technology are also applicable to the present invention.
Albumin successfully comprises fungi as recombinant protein multiple host and (includes but not limited to Aspergillus (Aspergillus) (WO06066595), genus kluyveromyces (Kluyveromyces) (Fleer1991, Bio/technology9, 968-975), Pichia (Pichia) (Kobayashi1998TherapeuticApheresis2, 257-262) with yeast belong (Saccharomyces) (Sleep1990, Bio/technology8, 42-46)), bacterium (Pandjaitab2000, J.AllergyClin.Immunol.105, 279-285)), animal (Barash1993, TransgenicResearch2, 266-276) (include but not limited to potato and tobacco (Sijmons1990 with plant, Bio/technology8, 217andFarran2002, TransgenicResearch11, expressed 337-346).Variant polypeptide of the present invention is preferably produced by restructuring in suitable host cell.In principle, any host cell that can produce polypeptide with appropriate amount all can use, and those skilled in the art have the ability selecting Suitable host cells according to the present invention.Preferred host living beings is yeast, is preferably selected from yeast belong (Saccharomycacae), is more preferably yeast saccharomyces cerevisiae.
Known isolation technique can be used as filtered, the combination of centrifugal, chromatography and Human serum protein technology etc. reclaims and purifying variant polypeptide of the present invention from growth medium.Those skilled in the art have use this type of known separating step particular combination purifying described in the ability of variant.As the example of purification technique that can be applicable to variant of the present invention, the instruction of WO0044772 can be mentioned.
Variant polypeptide of the present invention can be used for upper for treatment useful compound delivery to there being the animals or humans of these needs individual.The upper useful compound of this type for the treatment of includes but not limited to for diagnosing mark as multiple developing technique and the compound that easily detects; Pharmaceutically active compound is as medicine, or specific binding module is as antibody.Variant of the present invention even can be connected to compound useful in two or more different treatments, such as a kind of antibody and a kind of medicine, this gives combination molecule specific binding in the ability of required target, and thus this particular target is provided to the medicine connected of high density.
Fusion polypeptide
Albumin variants of the present invention and fragment thereof also can merge with non-albumin polypeptide fusion partner mutually.Described fusion partner can be any polypeptide in principle, but general preferred described fusion partner is the polypeptide with treatment or diagnostic feature.The fusion polypeptide comprising albumin or its fragment is well known in the art.Find that the fusion polypeptide that this kind comprises albumin or its fragment and fusion partner polypeptide has longer plasma half-time compared with the fusion partner polypeptide do not merged.According to the present invention, the plasma half-time of fusion polypeptide of the present invention relative to the corresponding fusion polypeptide of prior art can be changed.
One or more can be treated peptide fusion to hold in albuminous N, C holds, and inserts the ring (loop) in albumin structure, or its arbitrary combination.It can comprise or not comprise joint sequence fusion polypeptide various ingredients separated.
The instruction relating to the fusion of albumin or its fragment is known in the art, and one skilled in the art will recognize that this type of instruction is also applicable to the present invention.WO2001/79271A and WO2003/59934A also comprises the example of the treatment polypeptide that can be blended in albumin or its fragment, and these examples are also applicable to the present invention.
Conjugate
Albumin variants of the present invention or its fragment can use technology as known in the art to put together in the second molecule.Described second molecule can comprise diagnostic module, and in this embodiment described in put together the diagnostic tool that can be used as in development, or described second molecule can be therapeutic compound, and described conjugate can be used for therapeutic purpose in this embodiment, wherein said conjugate has the treatment characteristic of described therapeutic compound and albuminous long plasma half-time.The conjugate of albumin and treatment molecule is well known in the art, and has verified as this, and this type of conjugate has longer plasma half-time compared with unconjugated free treatment molecule.The free sulphur group (amino-acid residue 34 of ripe HSA) that described conjugate can exist on the surface conveniently by HSA uses known chemical process to connect.
In particularly preferred at one, put together described variant albumin and fragment thereof in useful therapeutic compound, and this conjugate is used for the treatment of the symptom had in the patient of these needs, wherein said symptom responds this therapeutic compound selected especially.For by this kind of therapeutic compound, the technology of puting together in variant albumin or its fragment is well known in the art.WO2009/019314 discloses the example being suitable for therapeutic compound being puted together the technology in polypeptide, and this technology is also applicable to the present invention.In addition, WO2009/019314 discloses the compound of Transferrins,iron complexes and the example of module that can put together in replacing, and these examples also can be applicable to the present invention.The instruction of WO2009/019314 is incorporated to herein by carrying stating.
HSA contains a free thiol group with its natural form, and it can be advantageously used in puting together.As the specific embodiments in this aspect, described variant albumin or its fragment can comprise the further modification be provided at its other free thiol group of Surface Creation.This has following benefit: the useful load of described variant albumin or its fragment is increased, thus the variant albumin therapeutic compound more than a molecule can puted together in per molecule or its fragment, maybe two or more different therapeutic compound can be puted together the variant albumin in per molecule or its fragment, such as, by the compound with target characteristic as to the such as specific antibody of tumour tool; And cytotoxic drug is puted together in variant albumin or its fragment, build the high degree of specificity medicine for tumour thus.Also be found in co-pending patent application WO2010/092135 to modifying with the instruction of the specific residue providing more free thiol group on surface, it is incorporated to herein by carrying stating.
Associated complex
Described albuminous variant or its fragment can use with the form of " associated complex " further.In the case, term " associated complex " is intended to mean to comprise albumin variants or its fragment and another kind and is connected by Non-covalent binding or associate in the compound of the another kind of compound of described variant albumin or its fragment.As the example of this kind of associated complex, can mention and to associate in the associated complex of albuminous lipid by variant albumin with by hydrophobic interaction.This type of associated complex is known in the art, and it can use known technology to prepare.As the example of the preferred associated complex of the present invention, the associated complex comprising variant albumin and taxol can be mentioned.
Other purposes
Variant albumin of the present invention or its fragment, or the fusion polypeptide comprising variant albumin or its fragment has and parent's albumin or its fragment, or the fusion polypeptide comprising parent's albumin or its fragment is compared, the benefit that its plasma half-time changes.Its benefit is of the present inventionly comprise the conjugate of variant albumin or its fragment or comprise the fusion polypeptide of variant albumin or its fragment, or the plasma half-time of the associated complex comprising variant albumin or its fragment can be selected according to concrete therapeutic purpose.
For example, for the conjugate for object of developing in animals or humans, associated complex or fusion polypeptide, when visualization module has very short half life, and comprise the conjugate of HSA or fusion polypeptide when having far beyond longer plasma half-time needed for this development object, use variant albumin of the present invention or its fragment compared with parent's albumin or its fragment with shorter plasma half-time, with provide have grow to for development object enough, but the conjugate being as short as the fusion polypeptide of the plasma half-time enough removed in its particular patient used is favourable.
At the conjugate of the therapeutic compound about the very pathology comprised in the patient of effectively treatment or this kind for the treatment of of alleviation needs, in another example of associated complex or fusion polypeptide, use and there is the variant albumin of longer plasma half-time or its fragment compared with parent's albumin or its fragment to provide the associated complex or conjugate with longer plasma half-time or fusion polypeptide can be favourable, this can have following benefit: associated complex of the present invention or conjugate or fusion polypeptide use the dosage not needing frequently to use or only need to reduce, thus with when use compared with the situation of parent's albumin or its associated complex or its fragment, there is less side effect.
In further at one, the present invention relates to and comprise variant albumin of the present invention, its associated complex or its fragment, variant albumin fragment or its associated complex, or comprise the composition of fusion polypeptide of variant albumin or its fragment.Described composition is preferably pharmaceutical compositions.Described composition can use technology known in the art to prepare, technology disclosed in handbook generally acknowledged in pharmaceutical field.
In a specific embodiment, described composition comprises variant albumin of the present invention or its fragment and comprises the compound of pharmaceutically useful module and albumin bound territory (ABD).According to the present invention, ABD means to be incorporated in vivo the albuminous site of circulation, module or territory, and make thus described ABD and any be incorporated into described ABD compound or module transport in the circulating cycle.ABD is well known in the art, and has shown it and be closely incorporated into albumin, therefore comprises the compound being incorporated into albuminous ABD and can show to a certain extent and to obtain picture individual molecule.The present inventor has recognized by variant albumin of the present invention or its fragment together being used with the compound comprising pharmaceutically useful module and ABD, this patient needed can be injected with wherein said compound like this, or situation about using in the preparation comprising native albumin or its fragment is compared, and changes the plasma half-time that this comprises the compound of pharmaceutically useful module and ABD.
Variant albumin of the present invention or its fragment, comprise the conjugate of variant albumin or its fragment, or comprise the fusion polypeptide of variant albumin or its fragment, or the associated complex comprising variant albumin or its fragment also can use techniques well known in the art group to enter nanometer or micron particle.Preferred method for the preparation of the nanometer or micron particle that are applicable to variant albumin of the present invention or its fragment is disclosed in WO2004/071536, and it is incorporated to herein by carrying stating.
Composition
The present invention also relates to albumin variants or its fragment or comprises the fusion polypeptide of variant albumin or its fragment, or comprise the conjugate of albumin variants or its fragment, or the associated complex comprising albumin variants or its fragment is preparing the purposes in pharmaceutical compositions, wherein said albumin variants or its fragment or comprise the fusion polypeptide of variant albumin or its fragment, or comprise the conjugate of albumin variants or its fragment, or comprise the associated complex of albumin variants or its fragment and HSA or its respective segments, or comprise the fusion polypeptide of HSA or its fragment, or the conjugate comprising HSA is compared, there is the plasma half-time of change.
In the case, the respective segments of described HSA is intended to the albuminous fragment of variant that means to compare with it to it and has an amino acid whose HSA fragment of equal amts.Similarly, the fusion polypeptide comprising HSA accordingly or the conjugate comprising HSA are intended to mean and comprise the molecule as the fusion polypeptide of the albuminous conjugate of the variant compared with formed objects and aminoacid sequence.
Preferably, described albumin variants or its fragment, or comprise the fusion polypeptide of variant albumin or its fragment, its fragment, the conjugate comprising albumin variants or its fragment and HSA or its respective segments or comprise HSA or its fragment fusion polypeptide plasma half-time compared with, there is higher plasma half-time.
Or, this can be expressed as: described albumin variants or its fragment, or comprise the fusion polypeptide of variant albumin or its fragment, its fragment, or the conjugate comprising albumin variants or its fragment for FcRn KD lower than to HSA or its respective segments or the corresponding KD of fusion polypeptide comprising HSA or its fragment.Preferably, for described albumin variants or its fragment, or comprise the fusion polypeptide of variant albumin or its fragment, its fragment, or the conjugate comprising albumin variants or its fragment, its KD is lower than the 0.9XKD of HSA, more preferably less than the 0.5XKD of HSA, more preferably less than the 0.1XKD of HSA, even more preferably less than the 0.05XKD of HSA, even more preferably less than the 0.02XKD of HSA, and most preferably lower than the 0.01XKD of HSA.
Described albumin variants or its fragment, or comprise the fusion polypeptide of variant albumin or its fragment, its fragment, or the conjugate comprising albumin variants or its fragment is preferably albumin variants of the present invention or its fragment, or comprise the fusion polypeptide of variant albumin or its fragment, its fragment, or the conjugate comprising albumin variants or its fragment.
The present invention is further described by following embodiment, and it should not be considered as limitation of the scope of the invention.
Embodiment
Materials and methods
ELISA:
Hole is used in the wild-type HSA or variant bag quilt that are diluted to given concentration in phosphate buffered saline (PBS) (PBS), is incubated overnight at 4 DEG C, then use 4% skimming milk (Acumedia) to close 1 hour in room temperature.Then by hole PBS/0.005%
20 (PBS/T) pH6.0 washs four times, then will be diluted in 4% skimming milk PBS/0.005% with the polyclone anti-GST antibody preincubation of the horseradish peroxidase (HRP)-put together from goat
the FEBSJ.2008Aug of 20 (PBS/T) pH6.0; () shFcRn (0.5 μ g/ml) of glutathione-S-transferase (GST) described in 275 (16): 4097-110-fusion is added into each hole, and at incubation at room temperature 1.5h, then wash four times with PBS/TpH6.0.Substrate tetramethyl benzidine (TMB) (Calbiochem) of 100 μ l is added into each hole, and incubation 45 minutes, then add the 0.25MHCl of 100 μ l.SunriseTECAN spectrophotometer (TECAN, Maennedorf, Switzerland) is used to measure absorbancy at 450nm.
Same ELISA is repeated with PBS/TpH7.4.
Surface plasmon resonance (SPR):
SPR experiment uses Biacore3000 device (GEHealthcare) to carry out.The amine conjugation chemistry described in the experimental program use manufacturer to provide the flow chamber (flowcell) of CM5 sensing chip and shFcRn-GST (~ 1400-5000RU) coupling.Coupling is undertaken by the albumen of 10 μ g/ml is injected 10mM sodium acetate pH5.0 (GEhealthcare).Use phosphate buffered saline buffer (67mM phosphate buffered saline buffer, 0.15MNaCl, 0.005% of pH6.0
20) as running buffer and dilution buffer.Surface regeneration uses the HBS-EP damping fluid (0.01MHEPES, 0.15MNaCl, 3mMEDTA, 0.005% tensio-active agent P20) injecting pH7.4 to carry out.In order to be incorporated into immobilized shFcRn-GST, often kind of HSA variant of 1.0-0.5 μM is injected with constant flow rate (40 μ l/ml) at 25 DEG C for surface.In all experiments, data are corrected (zeroadjust) by zeroing, and deduct with reference to number of chambers certificate.Data are weighed and are used BIAevaluation4.1 software (BIAcoreAB) to carry out.
Repeat same SPR by HBS-EP pH of buffer 7.4 to measure.
With regard to the object of this patent, unless stated otherwise, HSA, WTHSA, rHA refer to register the commercially available recombination human serum albumin of trade name RECOMBUMIN (can obtain from NovozymesBiopharmaUKLtd, NottinghamUK), and it is for embodiment.
Serum albumin from other species: these albumin, when mentioning, are restructuring, and the sequence using the obtainable database of the public to provide produces.Or it is what buy from commercial supplier.
FcRn: the expression and purification of soluble human (shFcRn) and mouse (smFcRn) FcRn:
For generating shFcRn and smFcRn expression plasmid, the method for each allodimer of expression and purification is found in (2005) J.Immunol.Methods298:93-104 such as Berntzen.Or shFcRnFcRn allodimer is produced by GeneArtAG (Germany).Sequence for two subunits of described allodimer is found in SEQIDNO:3 and SEQIDNO:4.Soluble receptors is expressed in HEK293 cell, and uses Ni-HiTrap column chromatography from culture supernatant.
Embodiment 1: the preparation of variant
The preparation of specific HSA mutain expression plasmid
Method for expressing HSA mutant variants and HSA fusion variant uses few techniques to produce.The Protocols in Molecular Biology of standard is adopted, as Sambrook, J.andD.W.Russell in whole process, 2001.MolecularCloning:alaboratorymanual, described in 3rded.ColdSpringHarborLaboratoryPress, ColdSpringHarbor, N.Y.
method 1: the aminoacid replacement in the HSA described in detail in Table 1
Synthetic DNA NcoI/SacI fragment (859bp) is by based gene assembly (GeneArtAG, Germany) generate, it includes point mutation to import required aminoacid replacement in the albumen of translation at HSA encoding gene (SEQIDNO:1).Table 2 details the codon for aminoacid replacement being imported HSA encoding gene.To encode the nucleotide sequence of synthesis fragment of unchanged amino acid (i.e. wild-type) and identical (being described in WO00/44772) in pDB2243.This synthetic nucleosides acid fragment is connected into the pDB2243 of NcoI/SacI digestion to produce plasmid pDB3876 – pDB3886 (table 1).For the generation of expression plasmid, by each for pDB3876 – pDB3886 (see table 1) personal NotI and PvuI digestion, DNA fragmentation is separated by 0.7% (w/v) TAE gel, and use QiagenGelExtractionKit to follow the instruction of manufacturer from agarose gel purification 2992bp fragment (' NotI box ', comprise PRB1 promotor, the DNA of encode fusion leading (FL) sequence (being disclosed in WO2010/092135), the nucleotide sequence of coding HSA, with ADH1 terminator, see Fig. 1)." disintegration (disintegration) " the plasmid pSAC35 ' NotI box ' being connected into NotI/ShrimpAlkalinePhosphatase (Roche)-process (is disclosed in EP-A-286424, and by Sleep, D., Deng (1991) Bio/Technology9, described in 183 – 187).Use and connect mixture conversion chemistry competence bacillus coli DH 5 alpha.Expression plasmid pDB3887 – pDB3897, the pSAC35 derivative containing " NotI box " uses standard technique qualification.Use disintegration plasmid pDB3887 – pDB3897 and pDB2244 (for expressing wild-type HSA, being described in WO00/44772) (table 1) transformed saccharomyces cerevisiae BXP10cir
0(as being described in WO/2001/079480 before, as mentioned below).
Table 1: plasmid, imports the aminoacid replacement of HSA
N/a=is inapplicable.PDB3876-pDB3886 is subclone plasmid.
Table 2: for aminoacid replacement being imported the codon of HSA
the generation of method 2:HSA modification D 494N+E495Q+T496A and E495Q+T496A
Adopt PCR-based, use the method for QuickChangeLightening test kit (Statagene) that point mutation is imported HSA.Oligonucleotide is used to generate two HSA variants (being respectively D494N+E495Q+T496A and E495Q+T496A) to xAP094 (SEQIDNO:5)/xAP095 (SEQIDNO:6) and xAP096 (SEQIDNO:7)/xAP097 (SEQIDNO:8).Use plasmid pDB3927 (being disclosed in WO2010/092135) as template DNA, and follow the method for kit manufacturer recommendation.By plasmid called after pDB3995 and pDB3996 (respectively containing HSAD494N+E495Q+T496A and E495Q+T496A expression cassette) of gained.PDB3995 and pDB3996 BstEII/BsrBI is digested, and the DNA molecular of straight chain is used standard technique purifying.The BstEII/BsrBI of each 100 ng is digested, uses QiagenPCR-Purification test kit to follow the DNA of the instruction purifying of manufacturer, the pDB3936 (being disclosed in WO2010/092135) digested with 100ngAcc65I/BamHI-respectively mixes, and for using the direct transformed saccharomyces cerevisiae BXP10cir of SigmaYeastTransformation test kit hereinafter described
0.
method 3: the aminoacid replacement in the HSA described in detail in table 3
Plasmid pDB3927 (being disclosed in WO2010/092135) (nucleotide sequence containing the coding HSA identical with pDB2243) is operated, makes, in ripe HSA albumen, aminoacid replacement occurs.Generate the DNA fragmentation (GeneArtAG of synthesis, Germany or DNA2.0Inc, USA) (NcoI/Bsu36I, AvrII/SphI or SacI/SphI fragment), includes point mutation required aminoacid replacement to be imported the protein sequence of translation at HSA encoding gene.Table 2 details the codon for aminoacid replacement being imported HSA encoding gene.The nucleotide sequence of synthesis fragment of unchanged amino acid (i.e. wild-type) of encoding is identical with those in pDB3927.Synthetic DNA fragment subclone is entered the pDB3927 (being described in PCT11527.204-WO) of NcoI/Bsu36I, AvrII/SphI-, SacI/Sph-digestion to generate pDB4006-pDB4010, pDB4083-pDB4101 and pDB4103-pDB4111 and pDB4194, pDB4200, pDB4202 (see table 3).
Similarly, BamHI/SalI fragment containing point mutation in the nucleotide sequence of coding HSA synthesizes (DNA2.0Inc by based gene assembly, USA), and connect into BamHI/SalI-digestion pDB3964 (being described in WO2010/092135) to produce plasmid pDB3986-pDB3989 (table 3).
Be those in rhesus monkey (PKFVAASQAALA) (SEQIDNO:10), mouse (PNLVTRCKDALA) (SEQIDNO:11), rabbit (PKLVESSKATLG) (SEQIDNO:12) and sheep (PKLVASTQAALA) (SEQIDNO:13) serum albumin by a string amino acid mutation of C end position 573-585 (KKLVAASQAALGL) (SEQIDNO:9) in HSA.Codon for importing each aminoacid replacement provides in table 2.Synthetic DNA fragment (SacI/SphI) is by based gene assembly synthesis (DNA2.0Inc, USA) (nucleotide sequence of synthesis fragment of unchanged amino acid (i.e. wild-type) of encoding and identical in pDB3927), and subclone enters the pDB3927 of SacI/SphI-digestion to generate plasmid pDB4114-4117 (table 3).
By plasmid pDB3883 (table 1), pDB4094 and pDB4095 (table 3) digests with NcoI/SacI, and by the 857bp fragment purification from each digestion, then connect into pDB4006 or pDB4110 (8.688kb) (table 3) of NcoI/SacI-digestion to produce pDB4156-pDB4161.
Generate expression plasmid in vivo and (namely in yeast saccharomyces cerevisiae, pass through homologous recombination, this technology is called breach reparation or vivo clone, see Orr-Weaver & Szostak.1983.Proc.Natl.Acad.Sci.USA.80:4417-4421).The plasmid BstEII/BsrBI listing in the modification of table 3 is digested, and uses the DNA molecular of standard technique purifying straight chain.The pDB3936 (being disclosed in WO2010/092135) that DNA that digested by the BstEII/BsrBI of each 100 ng, that use QiagenPCR-Purification test kit to follow the instruction purifying of manufacturer digests with 100ngAcc65I/BamHI-respectively mixes, and for using the direct transformed saccharomyces cerevisiae BXP10cir of SigmaYeastTransformation test kit hereinafter described
0.
Table 3.
Table 4:K500 primer and plasmid
Table 5:K573 primer and plasmid
method 4:HSAK500 and K573 arranges library
Use PCR to produce two arrangement libraries, wherein codon change (sudden change) of the amino acid 500 or 573 of encoding mature HSA is other non-wild-type amino acids and terminator codon (K5XXSTOP).Devise mutagenic oligonucleotide (table 4 and table 5) to increase HSA coding DNA group enters required change.That is, for the change at position 500 place, use pDB4082 (Fig. 1) as template DNA.PDB4082 is the derivative (being disclosed in EP1788084) of pDB2305, and generation as described below.By pDB2305 (Fig. 2) NsiI/SpeI digestion, and by the 8.779kbNsiI fragment produced from connecting to produce pDB4005 (Fig. 3).Synthetic DNA fragment (BsaI/SphI) generates (DNA2.0Inc by based gene assembly, USA) (SEQIDNO:1) (3rd ' district containing PRB1 promotor, the fusion leader modified, the nucleotide sequence of coding HSA, with the 5th ' district of ADH1 terminator modified), and the pDB4005 (Fig. 3) connecting into HindIII/SphI-digestion is to produce pDB4082.Remarks: removed the HindIII site in PRB1 promotor site and imported SacII site in the nucleotide sequence of coding HSA.
For the arrangement library of HSA position 500, the oligonucleotide listed in NewEnglandBiolabsPhusion test kit (table 6) and table 4 is used to generate the nucleotide sequence (see plasmid map pDB4082, Fig. 1) of the coding HSA corresponded between SalI/HindIII site.Table 7 describes the PCR method of employing.
In HSA, the arrangement library at amino acid position 573 place uses pDB3927 to generate as template DNA, and the oligonucleotide amplification relating to detailed description in use table 5 corresponds to the albumin coding DNA between NcoI and Bsu36I site.
Table 6:PCR composition
Table 7:PCR condition
For the albumin variants of position-based 500 and 573, each PCR primer is used QiagenPCR-scavenger reagent box (instruction according to manufacturer) purifying, with SalI/HindIII (library, position 500) or NcoI/Bsu36I (library, position 573) digestion.Then the DNA of digestion is used QiagenPCR-scavenger reagent box purifying, and connect into pDB4082 or pDB3927 of SalI/HindIII-or NcoI/Bsu36I-digestion respectively, substitute native sequences of equal value.Connector is transformed into bacillus coli DH 5 alpha, then uses Qiagen Miniprep Kit (instruction according to manufacturer) to be separated from transformant plasmid, and identify correct construct by restriction analysis.This generates plasmid set pDB4204 – pDB4222 (library, position 500) pDB4173 to pDB4190 (library, position 573), containing the albumin gene (being respectively table 4 and 5) that the sequence only corresponding to the amino acid whose codon at position 500 or 573 place at it there are differences.Concrete change in each plasmid is confirmed by order-checking.
The plasmid of gained is used to generate expression plasmid and the raw yeast of Albumin fusion produce by vivo clone as above.That is, use SigmaYeastTransformation test kit (as described below), use 100ng to contain the mixture transformed saccharomyces cerevisiae of the plasmid of the HSA variant of BstEII/BsrBI-digestion and the pDB3936 of 100ngAcc65I/BamHI digestion.
The conversion of yeast saccharomyces cerevisiae
By yeast saccharomyces cerevisiae BXP10cir
0(as being described in WO/2001/079480 before) or strains A cir
0(being described in WO/2005/061718) lines YEPD flat board (1% (w/v) yeast extract, 2% (w/v) Bactopeptone, 2% (w/v) glucose, 1.5% agar) on, and allow before conversion 30 DEG C of growths 4 days.By the full plasmid (i.e. cyclic plasmid) of a μ g, or for breach reparation, 100ng contains the HSA variant of BstEII/BsrBI-or NsiI/PvuI-digestion or the plasmid of HSA variant fusion thing, with lithium acetate method (the Sigmayeasttransformation test kit of pDB3936 for using SigmaYeastTransformation test kit to use amendment of 100ngAcc65I/BamHI digestion, YEAST-1, scheme 2; Ito etc. (1983) J.Bacteriol., 153,16; Elble, (1992) Biotechniques, 13,18) transformed saccharomyces cerevisiae.Described scheme is changed slightly, before heat shock, by conversion product incubation at room temperature 4 hours.After heat shock, cell is centrifugal momently, be then resuspended in 200 μ l1M sorbyl alcohols, be then plated on BMMD agar plate, the composition of BMMD is described in Sleep etc., (2001), Yeast, 18,403.By flat board 30 DEG C of incubations 4 days, then that single bacterium colony sticking patch is dull and stereotyped to fresh BMMD.Yeast strain number is specified in table 1.
As described below for each yeast strain preparation deposit: the BMMD nutrient solution each yeast sticking patch of one large ring is inoculated, and at 30 DEG C of growth 24h, accompany by the set pattern vibration of 200rpm.Cell, by within centrifugal 5 minutes, gathering in the crops with 1900xg in SorvalRT600 whizzer, removes 15mL supernatant, and substitutes with trehalose 40% (w/v).Cell is resuspended and be transferred to cryovial (1mL) at-80 DEG C of storages.
The Shake flask grown of yeast saccharomyces cerevisiae
By BMMD, (formula: 0.17% (w/v) does not contain the yeast nitrogen base (Difco) of amino acid and ammonium sulfate, 37.8mM ammonium sulfate, 29mM citric acid, 142mM dehydration Phosphoric acid disodium dodecahydrate pH6.5,2% (w/v) glucose) the every primary yeast inoculation of substratum (10mL), and at 30 DEG C of growth 12h, accompany by the vibration of 200rpm set pattern.By the aliquots containig (4mL) of each initial cultivation for inoculating 2x200mLBMMD substratum, and at 30 DEG C of growth 36h, accompany by the vibration of 200rpm set pattern.Cell by gathering in the crops via 0.2 μm of vacuum filtration film (Stericup, Millipore) (comprising GF-D prefilter) filtration, and retains supernatant for purifying.
Elementary concentrated
TangentialFlowFiltration is used to use the PallFiltronLV system of configuration Omega10KD (0.093sq.m2) filter (LVCentramateTMcassette, PallFiltron) with the transmembrane pressure of 20psi and 180mL.min the culture supernatant retained
-1recirculation rate concentrate.
Fermentation
Fed-batch fermentation is carried out in the 10LSartoriusBiostatC fermentation container of 30 DEG C; Monitor pH, and by suitably adding ammonia or sulfuric acid adjusts pH.Described ammonia also provide for the nitrogenous source of culture.Monitor the level of dissolved oxygen, and itself and agitator speed are associated, to maintain the saturated level of >20%.Inoculum is growth in the minimum medium (formula) of buffering in flask.For batch phase, culture is inoculated into the fermentation container medium (about 50% fermentation container volume) containing 2% (w/v) sucrose.Feed phase is triggered automatically by the sharply rising of dissolved oxygen levels.By feed rate being controlled to the nominal growth rate of setting, sucrose is maintained growth limitation concentration.Feed supplement is made up of the fermentation media containing 50% (w/v) sucrose, all substantially, (Collins as described in Collins, S.H., (1990) Productionofsecretedproteinsinyeast, in:T.J.R.Harris (Ed.) Proteinproductionbybiotechnology, Elsevier, London, pp.61-77).
GP-HPLC is quantitative
By GP-HPLC and the albumin variants quantitatively analyzing purifying, fusions and conjugate as described below.25 μ L are injected for the long TSKG3000SWXL post (TosohBioscience) of 7.8mm internal diameter (id) x300mm with 6.0mm internal diameter (id) x40mm long TSKSW guard column (TosohBioscience).By sample at 25mM sodium phosphate, 100mM sodium phosphate, 0.05% (w/v) sodiumazide, carried out chromatography with 1mL/ minute in pH7.0.Sample by detecting to be come quantitatively by peak area with the rHA standard specimen relative to concentration known (10mg/mL) at the UV of 280nm, and is corrected with regard to its relative extinction coefficient.
From shaking flask purification of albumin variant
Albumin variants uses single chromatographic step to use albumin affinity matrix (AlbuPure from shaking flask (culture supernatant or concentrated culture supernatant)
tM-ProMeticBioSciences, Inc.) purifying.Chromatography carries out with the constant linear speed of 240cm/h all along.Culture supernatant is imposed on the 2.0mL packed bed of the 6cm height of bed with 50mM sodium acetate pH5.3 pre-equilibration.After loading, with the level pad of 10 column volumes (CV), then use 50mM ammonium acetate pH8.0 (10CV) washing column.Product 50mM ammonium acetate 10mM sad pH8.0,50mM ammonium acetate 30mM Sodium octoate 200mM sodium-chlor pH7.0 or 200mM potassium sulfocyanate wash-out.Post is cleared up with 0.5MNaOH (3cv) and 20mMNaOH (3.5cv).The concentrated eluate fraction from each albumin variants, and carry out diafiltration (diafilter) for the 50mM sodium-chlor of 10 volumes and (there is the Vivaspin2010 of optionally diafiltration cup (diafiltrationcup), 000MWCOPES, Sartorius).The albumin variants of purifying is quantitative by GP-HPLC as above.
From shaking flask purification of albumin fusions variant
Albumin fusion variant uses single chromatographic step to use albumin affinity matrix (AlbuPure from shake-flask culture supernatant
tM-ProMeticBioSciences, Inc.) purifying.Chromatography carries out with the constant linear speed of 240cm/h from start to finish.Culture supernatant or concentrated culture supernatant are imposed on the 2.0mL packed bed of the 6cm height of bed with 50mM sodium acetate pH5.3 pre-equilibration.After loading, with the level pad of 10 column volumes (cv), then 50mM ammonium acetate pH8.0 (10cv) washing column.Product 50mM ammonium acetate 10mM sad pH8.0,50mM ammonium acetate 30mM Sodium octoate 200mM sodium-chlor pH7.0,50mM ammonium acetate 100mM Sodium octoate pH9.0 or 200mM potassium sulfocyanate carry out wash-out.Post is cleared up with 0.5MNaOH (3cv) and 20mMNaOH (3.5cv).Elutriated fraction from each albumin variants fusions to be concentrated and for the 25mMTris of 10 volumes, 150mMNaCl, 2mMKCl, pH7.4 diafiltration (there is the Vivaspin2010 of optionally diafiltration cup, 000MWCOPES, Sartorius).The Albumin fusion thing variant of purifying is quantitative by GP-HPLC as above.
From fermentation purification of albumin variant
By use SorvallRC3C whizzer (DuPont) centrifugal be separated after, from high-cell density fed-batch fermentation supernatant purification of albumin variant.As mentioned above by culture supernatant via the synthesis albumin affinity matrix (AlbuPure with customization
tM-ProMeticBioSciences, Inc.) the 11cm height of bed post 8.6mL packed bed chromatography of filling.Used by product elution buffer as above with the flow velocity wash-out of 120cm/h.By GP-HPLC (see on) and reduction SDS-PAGE with regard to purity check elutriated fraction, and if desired, carry out concentrating (Vivaspin2010,000MWCOPES) and impose on Superdex75 fill 2.4x96cm post, and with the flow velocity of 39cm/h at 25mMTris, run in 150mMNaCl, 2mMKCl, pH7.4.By peak classification, measured by GP-HPLC, and collect, to generate the monomeric protein of target.By the fraction concentrated (Vivaspin2010,000MWCOPES, Sartorius) collected.
All acceptor to be determined (FcRn) binding characteristics and/or other albumen analyzed as described above by GP-HPLC quantitatively, and correct with regard to its relative extinction coefficient.
Embodiment 2: HSA and the albuminous acceptor of recombinant human (shFcRn) binding characteristic of determining blood sources
By the HSA (Sigma-Aldrich) that is substantially free of lipid acid further by such as (2010) .J.Biol.Chem.285 such as Andersen, (7), the size exclusion chromatography described in 4826 – 4836 carrys out purifying.Use SPR described above analyzes monomer HSA and rHA of ten μMs, and data are shown in Fig. 4.
Showing for two kind samples with recombinant human albumin (Recombumin) in directly comparing of same concentrations (10 μMs) (Fig. 4 A and 4B) by HSA (blood sources), is reversible and pH interdependence to the combination of immobilized shFcRn (being respectively pH6.0 and pH7.4).In addition, Bosse etc. (2005) ..J.Clin.Pharmacol.45; Albuminous to HSA and recombinant human comparison is shown it have identical half life in mankind's In vivo study by 57-67.
Embodiment 3: acceptor (shFcRn) binding characteristic determining albumin variants
Use the FcRn combination mensuration that two kinds generally acknowledged: ELISA and SPR.Have obvious difference between these assay methods: in ELISA system, HSA direct coated is on hole, and shFcRn-GST adds in the solution, and in SPR measures, shFcRn-GST is immobilized onto CM5 chip, and HSA injects in the solution.Between two kinds of systems, pH can there are differences.
ELISA is used to analyze variant at pH6.0 and pH7.4.Result is disclosed in Fig. 5.ELISA value represents the mean value of replication.
Use SPR to analyze and analyze variant at pH6.0 and pH7.4.For the variant of representative number, result is disclosed in Fig. 6 (using the variant concentration of 0.2 μM) and Fig. 7 (using the variant concentration of 1 μM).
The SPR data normalization (normalize) of Fig. 6 and Fig. 7 will be disclosed in, and variant is shown in Fig. 8 A and B in the relative combination of each concentration.
The conclusion of this analysis is, the variant of all tests has the integrate features to acceptor at pH6.0, but does not have combination at pH7.4.Modification D 494N, Q, A, E495Q, A, T496A and D494N+T496A and HSA compare the combination of display to the minimizing of acceptor.
Embodiment 4: the determination of albumin variants acceptor (shFcRn/smFcRn) binding characteristic
Use following SPR analytical procedure, people and mouse FcRn calculating association constant Ka, dissociation constant Kd and binding constant KD (table 8) are incorporated into HSA and mice serum albumin (MSA).
SPR analyzes-SPR analysis and carries out at the upper CM5 chip that uses of BIAcore3000 device (GEHealthcare), and the immobilization of smFcRn-GST and shFcRn-GST variant or smFcRn uses amine coupling reagent kit (GEHealthcare) to carry out.Completely as described in manufacturer, protein sample (10 μ g/ml) is injected the 10mM sodium acetate (GEHealthcare) of pH4.5.Unreacted module 1M thanomin on surface is closed.For all experiments, use phosphate buffered saline buffer (67mM phosphate buffered saline buffer, 0.15MNaCl, 0.005% at pH6.0 or pH7.4
20) or at pH7.4 use HBS-P damping fluid (0.01MHEPES, 0.15MNaCl, 0.005% tensio-active agent P20) as running buffer or dilution buffer.Kinetic measurement uses low density immobilization surface (100-200 resonance units (RU)) to carry out.By hIgG1 (2000.0-31.2nM), the serial dilution of mIgG1 (1000.0-15.6nM), MSA (20.0-0.3 μM) and HSA (200.0-3.1 μM) injects with the flow velocity of 50 μ l/ minutes at 25 DEG C at pH6.0 or pH7.4.Extra injected HSA (10 μM) with 20 μ l/ minutes for immobilized shFcRn (~ 600RU) or smFcRn (~ 600RU) at pH6.0 in combination with at 25 DEG C, MSA (5 μMs), one of hIgG1 (100nM) or mIgG1 (100nM) or two records.Competitive binding passes through shFcRn (50nM) or smFcRn (100nM) is independent or together measures for immobilized HSA (~ 2600RU) or MSA (~ 2000RU) from HSA or MSA (10.0-0.05 μM) of different amount.In all cases, in order to correct non-specific binding and a large amount of shock absorption (bulkbuffereffect), the response obtained from contrast surface and blank injection is deducted from each Curves of Interaction.Use model (Langmuir1:1 Ligand Model, heterologous ligand model and stable state avidity model) the computational dynamics rate value of determining in advance that BIAevaluation4.1 software provides.In all avidity estimations, by the statistical value χ representing root mean square value
2, the degree of closeness of the matching of description is lower than 2.0.
Table 8:HSA and MSA is to the binding constant of shFcRn and smFcRn
KD uses BIAevaluation4.1 Software Create.Use Langmuir1:1 Ligand Model from start to finish.Dynamics represents three mean values repeated.ND means not determine.NA means not obtain.
Embodiment 5: from the albumin of other species to the combination of people FcRn
By commercially available animal white albumen (Sigma-Aldrich or Calbiochem) further as (2010) .J.Biol.Chem.285 such as Andersen, (7), carry out purifying described in 4826 – 4836.Donkey serum albumin, bovine serum albumin, lowlenthal serum albumin, sheep serum albumin, albumin rabbit serum, dog serum albumin, hamster sera albumin, guinea pig serum albumin, rat serum albumin and the chicken serum albumin technology described in materials and methods that is combined to shFcRn is determined.ELISA result is disclosed in Fig. 9 A-D, and relatively combines and be summarized in Fig. 9 E.
SPR result is shown in Figure 10, which show the combination of often kind of albumin species at pH6.0 and pH7.4.Figure 10 shows the outline of the relative combination response using ELISA with SPR to record:
Table 10: between species, albumin-FcRn combines
Relative combination response classifies as the most by force (++++) to the most weak (+) with without combination.
A: do not determine (ND).
As follows from the strongest ranking compositor to most weak binding: cavy=/> rabbit > hamster/dog > rat/mouse > donkey > people > ox > goat/sheep > chicken.This data presentation animal white albumen has different avidity to shFcRn.
Embodiment 6:HSA variant is to the kinetics of shFcRn
The binding constant of variant of the present invention is determined according to the method described in materials and methods.
Table 11:HSA variant is to the binding constant of shFcRn
KD uses BIAevaluation4.1 Software Create.Use Langmuir1:1 Ligand Model from start to finish.Dynamics represents three mean values repeated.ND means not determine.
Result corresponds to the conclusion drawn based on SPR and ELISA data in embodiment 3, but also shows E492G has increase avidity to its acceptor.
The competitive analysis of embodiment 7:HSA variant
In embodiment 1, the HSA variant of preparation and the competitive analysis of WTHSA use the method described in embodiment 4 to carry out.Result is shown in Figure 15.
Result display variant E492G and HSA compares shFcRn and has stronger combination, unlike E492H, E492P and E492G+V493P.
The analysis that embodiment 8:Q417 replaces
Use the method for embodiment 1, construct the variant with the HSA replacing Q417A and D494E+Q417H.Use the method in materials and methods to test the dynamics of these variants, and be shown in table 12.
Table 12:HSA variant is to the binding constant of shFcRn
The diluting needle of a:HSA variant is injected immobilized shFcRn (~ 1500RU).
B: Kinetics Rate Constants By Using uses simple one-level (1:1) bio-molecular interaction model to obtain.
C: balance (Req) combination model that stable state affinity costant uses BIAevaluation4.1 software to provide obtains.Dynamics represents three mean values repeated.
D: do not determine (ND).
Data presentation variant Q417A with D494E+Q417H is compared with wild-type HSA, more weak with the combination of acceptor.
Embodiment 9: in the analysis of the HSA variant at position 499,500,536,537,538 and 573 place
Use the method for embodiment 1, construct the HSA variant having and replace P499A, K500A, K536A, P537A, K538A and K573A.The receptor-binding characteristic of these variants is tested as described in materials and methods.Result is shown in Figure 11.
Data show that variant P499A, K536A, P537A and K538A have the binding affinity of minimizing to shFcRn relative to HSA.Relative to HSA, variant K500A almost completely loses its ability in conjunction with shFcRn, and K573A has the binding affinity of increase to shFcRn.
The analysis of the variant of embodiment 10:HSA position 501
Use the method for embodiment 1, construct the HSA variant having and replace E501A and E501Q.The dynamics of these variants is tested as described in materials and methods.
Table 13:HSA variant is to the binding constant of shFcR
A: the diluting needle of HSA variant is injected immobilized shFcRn (~ 1500RU).
B: Kinetics Rate Constants By Using uses simple one-level (1:1) bio-molecular interaction model to obtain.
C: balance (Req) combination model that stable state affinity costant uses BIAevaluation4.1 software to provide obtains.Dynamics represents three mean values repeated.
D: do not determine (ND).
Data presentation variant E501A and E501Q has the binding affinity slightly reduced to shFcRn relative to HSA.
Embodiment 11: the analysis of the HSA variant of position 573
Use the method for embodiment 1, construct the variant in position 573 with the HSA of replacement.Generate all variants at position 573 place, and as described in materials and methods, test the receptor-binding characteristic of these variants, but SPR analysis is carried out at pH5.5.Result is shown in following table 14 and Figure 12 and 13.
The kinetics of table 14:HSAK573 single-point mutants
A: the diluting needle of HSA variant is injected immobilized shFcRn (~ 1500RU).
B: Kinetics Rate Constants By Using uses simple one-level (1:1) bio-molecular interaction model to obtain.
C: balance (Req) combination model that stable state affinity costant uses BIAevaluation4.1 software to provide obtains.Dynamics represents the mean value of twice repetition.
D: do not determine (ND).
The all variants in position 573 with replacement of result display have the combination of improvement compared with WTHSA.Specifically, variant K573F, K573H, K573P, K573W and K573Y and parent HSA compares shFcRn and has the KD being low to moderate and being less than 1/10.Variant K573STOP is the albumin in position 573 with the brachymemma of terminator codon.To the induction spectrum combination that display significantly reduces compared with WTHSA of K573STOP variant, and generate higher KD.The avidity that the variant K573E (natural variant characterized by Otagiri (2009) .Biol.Pharm.Bull.32 (4) 527-534) of our display increases is estimated to add its half life in vivo.
Embodiment 12: to the analysis of further HSA variant
Use the method for embodiment 1, construct and there is replacement E492G, E492G+N503H, N503H, D550E, E492G+N503K, E542P, H440Q, K541G, K541D, D550NE492G+K538H+K541N+E542D, E492T+N503K+K541A, E492P+N503K+K541G+E542P, the HSA variant of E492H+E501P+N503H+E505D+T506S+T540S+K541E, A490D+E492T+V493L+E501P+N503D+A504E+E505K+T506F+K541D, E492G+V493P+K538H+K541N+E542D.As described in materials and methods, test the receptor-binding characteristic of these variants, and show the result in table 15 and Figure 14.
Table 15:HSA variant is to the binding constant of shFcR
A: the diluting needle of HSA variant is injected immobilized shFcRn (~ 1500RU).
B: Kinetics Rate Constants By Using uses simple one-level (1:1) bio-molecular interaction model to obtain.
C: balance (Req) combination model that stable state affinity costant uses BIAevaluation4.1 software to provide obtains.Dynamics represents three mean values repeated.
D: do not determine (ND).
Result display, for position 550, is substituted by the avidity that E causes increasing shFcRn at pH6.0, and is substituted by N and decreases avidity.But, when repeating this analysis at pH5.5 to D550E replacement, do not find that observable avidity increases.Be substituted by acidic amino acid (E) keep and improve combination.But, be substituted by not charged amidoamino acid and decrease combination at pH6.0.Based on this observation, we can predict the further minimizing being substituted by basic aminoacids (H, K and R) and can causing combining for this position.
Sudden change in embodiment 13:His residue
The method described in embodiment 1 of use generates following variant: H440Q, H464Q, H510Q and H535Q.Figure 15 shows these variants, and as described in materials and methods, interactional SPR responds to and composes with shFcRn.
Find that variant H440Q is combined with the avidity similar with HSA.In contrast, H464Q, H510Q and H535Q have the avidity significantly reduced to shFcRn.This is disclosed observation before supporting, namely the mutagenesis of these histidine residues significantly reduces the combination (Wu etc. (2010) .PEDS, 23 (10) 789 –s 798) of HSA to shFcRn.Wu etc. show the minimizing for the half life of Diabody in mouse (diabody) fusion rotein (scFv-DIII) 2, according to arriving the order removed the soonest the most slowly: Db-DIIIWT>H535A>H510AGreatT. GreaT.GTH464A>Db.Based on the avidity to shFcRn, and when compared with smFcRn (embodiment 5), we can predict removing order in the mankind is (replacing for glutamine (Q)): WT>H440Q>H510QGreatT.GreaT.G TH464Q>H535Q.
Embodiment 14: further variant
The method described in embodiment 1 of use generates following variant: K574N and Q580K in HSA.Use the SPR described in materials and methods to measure test variant to the combination of FcRn, and result is shown in table 16.
Result display variant K574N and Q580K combines stronger to shFcRn.
Table 16: following dynamics data is found to these variants:
| Albumin variants | ka | kd | KD |
| (10 3/Ms) | (10 -3/s) | (μM) | |
| WT | 9.7±0.0 | 30.0±0.1 | 3.1 |
| K574N | 4.9±01 | 8.4±0.1 | 1.7 |
| Q580K | 6.0±0.0 | 9.3±0.0 | 1.5 |
Embodiment 15: in the analysis of the HSA variant of position 500.
The method of embodiment 1 is used to construct the HSA at position 500 place with replacement.Generate all variants at position 500 place, and test the receptor-binding characteristic of these variants.Use BiacoreX, BiacoreX100 and SensorChipCM5 for all analyses, it provides by GEHealthcare.The shFcRn produced by GeneArtAG (Germany) (being diluted to 10 μ g/mL in 10mM sodium acetate pH5.0 (GEHealthcare)) is immobilized onto on flow chamber 1 (FC1) to 1600-2200 response units (RU) via standard amine coupling according to the instruction (GEhealthcare) of manufacturers.Flow chamber 1 (FC1) carries out blank immobilization to serve as with reference to room to it.In order to stable mensuration, first only use running buffer (67mM phosphate buffered saline buffer, 0.15MNaCl, 0.005%Tween20, in pH5.75 ± 0.25) to run 3-5 initial cycles, then regenerate.WTrHA and K500 library variant 90s is injected with constant flow rate (30 μ l/min) with multiple concentration (1 μ M – 150 μMs) at 25 DEG C, then HBS-EP pH of buffer 7.4 (GEHealthcare) regenerating surface is used, until recovered roughly initial baseline RU (usual 12s pulse is namely enough).
Result is shown in table 17 and Figure 16.
The kinetics of table 17:HSAK500 single-point mutants
The mean value of a:4 value.
B: Kinetics Rate Constants By Using uses simple one-level (1:1) bio-molecular interaction model to obtain.
C: balance (Req) combination model that stable state affinity costant uses BIAevaluation4.1 software to provide obtains.
Result shows, and variant K500R compares with K500I and WTHSA, that have increase respectively to shFcRn with similar avidity.Variant K500E combines closely in immobilized shFcRn, but still the interactional feature pH interdependence of display FcRn.This mixture is highly stable, thus dynamic analysis cannot be carried out (Figure 16).Every other variant and wtrHA compare the combination that shFcRn has minimizing.
All variants are incorporated into shFcRn (to a certain extent) at pH5.5.Cannot detect that K500 library variant is to the combination of shFcRn at pH7.4.
Embodiment 16: fusion polypeptide
The generation of the Albumin fusion thing containing albumin mutain
Modify and (be described in Evans etc. in HSA for the plasmid of expression cassette producing scFv (vHvL) containing in N end or C end or two ends gene fusion, 2010.ProteinExpressionandPurification.73,113 – 124) to allow to use vivo clone (as described above) to produce Albumin fusion thing.By pDB3017 (Figure 17), pDB3021 (Figure 18), pDB3056 (Figure 19) NsiI/SpeI digestion, and use standard technique purifying to correspond respectively to 9.511kb, the NsiI fragment of 9.569kb and 8.795kb.By the NsiI fragment of purifying from connecting, and use it for conversion chemistry competence bacillus coli DH 5 alpha to produce pDB4168 respectively, pDB4169 and pDB4170 (table 18).
Similarly, pDB3165 (containing bivalent fusion thing) (Figure 20) is digested with NotI, and purifying expression cassette (4.506kb fragment), then connect in NotI-digestion pDB3927 to produce pDB4172 (Figure 21, table 18).
By based gene assembly (GeneArtAG, Germany) generate the SalI/Bsu36IDNA fragment (269bp) of synthesis, it includes point mutation the aminoacid replacement corresponding to K500A or D550N or K573P to be imported the albumin protein sequence of translation at albumin coding nucleotide sequence.SalI/Bsu36I fragment is connected to separately pDB4168-pDB4170 and pDB4172 of SalI/Bsu36I-digestion, and uses its conversion chemistry competence bacillus coli DH 5 alpha of standard technique to generate plasmid pDB4265-pDB4276 (table 18).
Table 18: albumin variants fusions
Similarly, generate DNA fragmentation by PCR (use standard technique), replace to import K573A in the albumin protein sequence of translation.PCR uses NewEnglandBiolabsPhusion test kit to use pDB4267 (Figure 22) to carry out as template DNA and oligonucleotide xAP238 (SEQIDNO:53) and xAP239 (SEQIDNO:54):
Table 19 describes PCR circulation.
Table 19:PCR circulation
By PCR primer purifying, with SalI/Bsu36I digestion, and the fragment (269bp) be separated is connected into pDB4168-pDB4170 and pDB4172 of SalI/Bsu36I-digestion, and use it for conversion chemistry competence bacillus coli DH 5 alpha.The plasmid (pDB4277-pDB4280) of gained lists in table 18.
The nucleotide sequence of coding FLAG mark is removed from plasmid pDB4168 and pDB4268-4270 (be respectively used to express N end and be blended in HSA and HSA mutain K500A, the plasmid of the scFv of D550N and K573P).PDB4168 and pDB4268-4270 (table 18) is comprised the product of the 231bp in HSA encoding gene 3 ' district with removal with Bsu36I/SphI digestion, the nucleotide sequence of coding FLAG mark and 5th ' district of ADH1 terminator.Standard technique is used to be connected to pDB4168 and pDB4268-pDB4270 of Bsu36I/SphI-digestion the Bsu36I/SphI fragment (207bp) comprised from 3rd ' district of the HSA encoding gene of pDB4181 and the 5' district of mADH1 terminator (SEQID1).Use and connect mixture use standard technique conversion chemistry competence bacillus coli DH 5 alpha to generate plasmid pDB4281-pDB4284 (table 18).
PDB4265-pDB4284 BstEII/BsrBI is digested, and uses the DNA molecular of standard technique purifying straight chain.100 ngBstEII/BsrBIDNA samples are mixed with the pDB3936 that 100ngAcc65I/BamHI-digests, and uses hereinafter described SigmaYeastTransformation test kit its transformed saccharomyces cerevisiae BXP10cir
0.In each case, in yeast, expression plasmid is generated by the homologous recombination (vivo clone) between the Albumin fusion thing containing plasmid (pDB4265-pDB4280) (table 18) and pDB3936.
By plasmid pDB3017, pDB3021, pDB3056 and pDB3165 is (by Evans etc., 2010.ProteinExpressionandPurification.73, the wild-type HSA fusions described in 113 – 124) for using SigmaYeastTransformation test kit transformed saccharomyces cerevisiae strains A cir as described below
0(being described in WO/2005/061718).
The nucleotide sequence of encoding human IL-1RA (interleukin-1 receptor antagonist) (accession number: CAA59087) is generated synthetically by based gene assembly.The nucleotide sequence (Bsu36I/SphI fragment) that 708bp synthesizes fragment provides in SEQIDNO:55, and comprise 3rd ' district of the gene of coding HSA, the nucleotide sequence of coding GS joint, the nucleotide sequence (glycoylation motif that N84Q connects to eliminate N occurs) of encoding human IL-1RA and 5th ' district of ADH1 terminator.Synthetic DNA fragment can be connected into the pDB3927 of Bsu36I/SphI-digestion to produce pDB2588.
Preparation as described below contains for generation of gene fusion in HSA and HSA variant K500A, the plasmid of the expression cassette of the IL-1RA of the C end of D550N, K573A and K573P.PDB2588 Bsu36I/SphI is digested, and use standard technique purifying to contain the 705bp fragment in 3rd ' district of HSA encoding gene, the nucleotide sequence of coding GS joint, the nucleotide sequence of encoding human IL1-RA (N84Q), with the 705bp fragment in 5th ' district of the yeast saccharomyces cerevisiae ADH1 terminator (SEQID3) modified, then the pDB4006 (containing HSAK573A expression cassette) into Bsu36I/SphI-digestion is connected, pDB4010 (containing HSAD550N expression cassette), pDB4086 (containing HSAK500A expression cassette), pDB4110 (containing HSAK573P expression cassette) is to generate pDB4287 respectively, pDB4286, pDB4285 and pDB4288 (such as, see Figure 23).PDB4285-pDB4288 NsiI/PvuI is digested, and uses the DNA molecular of standard technique purifying straight chain.(namely the pDB3936 (9721bp) that the DNA sample of 100 ngNsiI/PvuI-digestion digests with 100ngAcc65I/BamHI-is mixed, vivo clone), and for using SigmaYeastTransformation test kit transformed saccharomyces cerevisiae (that is, passing through vivo clone) as described below.
The preparation that expressing gene is blended in the Wine brewing yeast strain of the wild-type HSA (see table 18) of GS joint and IL1-RA (N84Q) also can follow method as above generation.
Use SPR method as above with regard to its binding analysis fusion polypeptide to FcRn, and obtain following result:
Table 20:HSA merges the kinetics of variant
A: the diluting needle of HSA variant is injected immobilized shFcRn (~ 1500RU).
B: Kinetics Rate Constants By Using uses simple one-level (1:1) bio-molecular interaction model to obtain.Dynamics represents the mean value of twice repetition.
C: do not determine due to weak binding (ND).
In embodiment 8, display K500A variant also indistinctively in conjunction with shFcRn, in embodiment 10, shows K573P with K573A variant more strongly in conjunction with shFcRn compared with HSA, and in embodiment 11, shows D550N variant compared with HSA weaker in conjunction with FcRn.
In the present embodiment, the difference showing the binding characteristic that these are observed also is reflected in the fusion polypeptide of different configuration: hold fusions (HSA-FLAG) with the C of little module, holds fusions (HSA-IL1RA) with the C of larger polypeptide; Fusions (scFv-HSA) is held with the N of polypeptide; N and C holds fusions (scFv-HSA-FLAG and scFv-HSA-scFv-FLAG).
Embodiment 17: horseradish peroxidase albumen is puted together albumin and K573P variant
For puting together analysis, use commercially available recombinant albumin (Recombumin
tM) with comparing molecule.For this embodiment, pass through the albumin K573P variant of the present invention of the final 200mg/mL of means purifying described in materials and methods from fed-batch fermentation.Carry out two-step purifying.
The post (the about 400mL of bed volume, height of bed 11cm) that the first step use AlbuPureTM matrix (ProMetic) fills.Used 50mM sodium acetate, pH5.3 balances, and is loaded on about 20mg/mL matrix by clean culture supernatant at about pH5.5-6.5.Then the 50mM sodium acetate pH5.3 of approximately each 5 column volumes is used respectively, 50mM sodium phosphate pH6.0,50mM sodium phosphate pH7.0 and 50mM sodium acetate pH8.0 washing column.Use the 50mM sodium acetate of about two column volumes, the albumen of the sad pH7.0 elution of bound of 10mM.The flow velocity of whole purifying is 154mL/ minute.
For second step, by the about twice of eluate dilute with water from the first step to obtain the specific conductivity of 2.5 ± 0.5mS/cm after being adjusted to pH5.5 ± 0.3 with acetic acid.Loaded on and use 80mM sodium acetate, 5mM is sad, DEAE-SepharoseFastFlow (GEHealthcare) post (the about 400mL of bed volume, height of bed 11cm) of pH5.5 balance.Be loaded as about 30mg albumen/mL matrix.With the 80mM sodium acetate of about 5 column volumes, the sad pH5.5 washing column of 5mM.Continue with the 15.7mM potassium tetraborate pH9.2 of about 10 column volumes.Use the 110mM potassium tetraborate of about pH9.0 of two column volumes, the albumen of 200mM sodium-chlor elution of bound.In loading and washing step process, flow velocity is 183mL/ minute, and in elution step process, be 169mL/ minute.
Eluate is concentrated, and uses PallCentramateOmega10,000NominalMWCO film for 145mMNaCl diafiltration, to obtain the final protein concentration of about 200mg/mL.
The phosphate buffered saline (PBS) (PBS) that albuminous for rHA and K573P variant 200mg/mL liquid storage uses pH to be adjusted to pH6.5-6.7 is diluted to 5mg/mL.This guarantees
under the maleimide reactive group of MaleimideActivatedHorseradishPeroxidase (ThermoScientific) is in favourable pH environment, with free sulfhydryl radical reaction, to form stable thioether bond.By 2mg's
maleimideActivatedHorseradishPeroxidase (HRP) mixes with 5mg/mLrHA or the K573P variant albumin of 1mL.This mixture guarantees albumin or the albuminous about twice molar excess of K573P variant.By this mixture 4 DEG C of incubations at least 24 hours.Then GP-HPLC is used just to put together inspection reaction mixture.
In order to be separated unconjugated species (rHA from puting together species accordingly, or albumin variants K573P and unreacted HRP), first by sample concentration (Vivaspin20,10,000MWCOPES, Sartorius), then TricornSuperdex is imposed on respectively with the flow velocity of 45cm/hr in PBS
tM200,10/300GL post (GEHealthcare).By elution peak classification, and carry out GP-HPLC analysis.Fraction containing the species puted together is collected, concentrated and for 50mMNaCl diafiltration, and analyzed by GP-HPLC and carry out presenting (Figure 24).
Then use described Biacore method to measure these samples (table 21) herein.This embodiment display K573P keeps the avidity that it increases for shFcRn compared with WTHSA.
Embodiment 18: fluorescein is puted together in albumin and K573P variant
For the identical albumin sample of two in embodiment 17, be also similarly the parent material of the present embodiment.That is, about 200mg/mLrHA or K573P albumin variants.
By fluorescein-5-maleimide, ThermoScientific (F5M) is dissolved in dimethyl formamide, to obtain the final concentration of 25mg/mL.Then it is diluted further the PBS into 18ml, pH is adjusted to about pH6.5.The 200mg/mLK573P variant of 200mg/mLrHA or 1mL of 1ml is added to this solution.This provide the F5M of about 20 times of final molar excess.These samples of incubation, and allow it to put together 4 DEG C of lucifuges to spend the night, to allow the maleimide base group on F5M and to be present in two kinds of albumin species the sulfhedryl being essentially free and to react.
After Overnight incubation, by the aliquots containig of reaction mixture for the thorough diafiltration of 50mMNaCl to remove unconjugated F5M (Vivaspin20,10,000MWCOPES, Sartorius).Put together and to be developed the fluorescein puted together by ultraviolet after the SDS-PAGE of standard:: albumin confirms (Figure 25).
Then use described Biacore method to measure these samples (table 21) through diafiltration herein.Small molecules is puted together by the explanation of this embodiment does not affect the trend of the binding affinity to shFcRn in rHA or variant such as K573P.
Table 21: when being incorporated into immobilized shFcRn, the representative Biacore of the rHA puted together or variant (K573P) measures KD value.
| Analyte | KD(μM) |
| rHA::HRP | 3.6 |
| K573P::HRP | 0.02 |
| rHA::F5M | 7.3 |
| K573P::F5M | 2.5 |
Embodiment 19: further albumin variants
The method described in embodiment 1 of use generates following variant: E492T, N503D, E492T+N503D, K538H, E542D, D494N+E495Q+T496A, E495Q+T496A, N403K, K541A and K541N.SPR analyzes and carries out as described in example 15 above, and the results are shown in Figure 26 and Figure 27.
Figure 30 A and 30B shows the effect that albumin variants combines shFcRn.
Replacement N503D in HSA, D494N+E495Q+T496A, E492T+N503D, E495Q+T496A have negative effect in the combination of pH5.5 couple and shFcRn.
Embodiment 20: at the albumin variants of C end
The method described in embodiment 1 of use generates following variant.The combination of shFcRn is determined as described in materials and methods, and the results are shown in table 22.
The variant that table 22:HSAC end exchanges and the interactional kinetics of shFcRn
A: the diluting needle of HSA variant is injected immobilized shFcRn (~ 1500RU).
B: Kinetics Rate Constants By Using uses simple one-level (1:1) bio-molecular interaction model to obtain.
C: from the data of table 2
D: from the data of table 3
Do not determine due to weak binding (ND)
This embodiment shows holding with the C of human albumin of all tests is exchanged to (swap) and observed and increase relative to the albuminous combination of donor.All remarkable combinations increased of donor sequences display replaced containing K573P, but be less than K573P itself (table 20).
Embodiment 21: the competitive binding analysis of variant Albumin fusion thing
Variant Albumin fusion thing and the albuminous competitive binding research of the selected variant prepared as described in example 1 above is used to carry out as described in example 4 above.Result is shown in Figure 28-31.
Hold the ranking compositor of competitive binding ranking compositor and the single HSA variant of scFvHSA-FLAG (do not merge with merge) affinity data identical for variant fusion thing HSA-FLAG with N+C.For IL1Ra variant K573P, K573A with K500A is identical with prediction, but D550N seems to merge than WT more effectively to suppress.
Embodiment 22: further HSA variant
The method described in embodiment 1 of use generates following variant: HSAE492G+K573A, HSAE492G+N503K+K573A, HSAE492G+N503H+K573A, HSAE492G+K573P, HSAE492G+N503K+K573P, HSAE492G+N503H+K573P.SPR analysis is carried out as described in materials and methods.It is stronger relative to the combination of wild-type HSA to shFcRn that result (Figure 32) is presented at pH5.5 all HSA variant.Do not observe combination at pH7.4.
HSAE492G+K573A, HSAE492G+N503K+K573A almost do not improve combination (hadmarginallyimprovedbindingbeyondthatofHASK573A), unlike HSAE492G+N503H+K573A relative to HSAK573A.Combinatory variants containing K573P does not show the combination of improvement relative to the single variant of K573P.
Claims (31)
1., for the preparation of albumin variants, its fragment or the method for fusion polypeptide comprising described variant albumin or its fragment, comprise the steps:
A. coding and SEQIDNO:2 is provided to have the albuminous nucleic acid of parent of at least 80% sequence iden;
B. the sequence of modification step a has the variant albumin of one or more replacement, its fragment with coding or comprises the fusion polypeptide of described variant albumin or its fragment, and described replacement corresponds in SEQIDNO:2 and is selected from following replacement:
Q417A,C,D,E,F,G,H,I,K,L,M,N,P,R,S,T,V,W,Y；
H440A,C,D,E,F,G,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
A490C,D,E,F,G,H,I,K,L,M,N,P,R,S,T,V,W,Y；
E492A,C,D,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
V493A,C,D,E,F,G,H,I,K,L,M,N,P,Q,R,S,T.W,Y:
D494A,C,E,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
E495A,C,D,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
T496A,C,D,E,F,G,H,I,K,L,M,N,P,Q,R,S,V,W,Y；
P499A,C,D,E,F,G,H,I,K,L,M,N,Q,R,S,T,V,W,Y；
K500A,C,D,E,F,G,H,I,,L,M,N,Q,R,S,T,V,W,Y；
E501A,C,D,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
N503A,C,D,E,F,G,H,I,K,L,M,P,Q,R,S,T,V,W,Y；
A504C,D,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
E505A,C,D,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
T506A,C,D,F,G,H,I,K,L,M,N,P,Q,R,S,V,W,Y；
H510A,C,D,F,G,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
H535A,C,D,F,G,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
K536A,C,D,E,F,G,H,I,L,M,N,P,Q,R,S,T,V,W,Y；
P537A,C,D,E,F,G,H,I,K,L,M,N,Q,R,S,T,V,W,Y；
K538A,C,D,E,F,G,H,I,L,M,N,P,Q,R,S,T,V,W,Y；
T540A,C,D,E,F,G,H,I,L,M,N,P,Q,R,S,,V,W,Y；
K541A,C,D,E,F,G,H,I,L,M,N,P,Q,R,S,T,V,W,Y；
E542A,C,D,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
D550A,C,E,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
K574A,C,D,E,F,G,H,I,L,M,N,P,Q,R,S,T,V,W,Y；
Q580A,C,D,E,F,G,H,I,K,L,M,N,P,R,S,T,V,W,Y；
A581C,D,E,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
A582C,D,E,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
G584A,C,D,E,F,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
H464A,C,D,E,F,G,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
L575A,C,D,E,F,G,H,I,K,M,N,P,Q,R,S,T,V,W,Y；
A577C,D,E,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
A578C, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, Y; Or
S579A,C,D,E,F,G,H,I,K,L,M,N,P,Q,R,T,V,W,Y；
C. the modification sequence in step b is imported suitable host cell;
D. allow described cell in suitable growth medium cause described albumin variants, its fragment or comprise described variant albumin or its fragment fusion polypeptide express condition under grow; And
E. reclaim described albumin variants, its fragment from described growth medium or comprise the fusion polypeptide of described variant albumin or its fragment,
Wherein said albumin variants, its fragment or the fusion polypeptide that comprises described variant albumin or its fragment and described parent's albumin, its fragment or comprise described variant albumin or its fragment fusion polypeptide compared with there is the plasma half-time of change.
2. method according to claim 1, wherein said albumin variants, its fragment or the fusion polypeptide comprising described variant albumin or its fragment have longer plasma half-time compared with described parent's albumin.
3. method according to claim 2, wherein albumin variants according to claim 3, its fragment or the fusion polypeptide that comprises described variant albumin or its fragment comprise the one or more replacements corresponding to replacement following in SEQIDNO:2:
K573P+K574N+A577T+A578R+S579C+Q580K+A581D+G584A，K573P+A577E+A578S+Q580K+A582T，K573P+A578S+S579T+G584A，K573P+L575F+G584A，E492G，N503K，K500R，N503H，D550E，K574N，Q580K，E492G+N503K，E492G+N503H，E492H+E501P+N503H+E505D+T506S+T540S+K541E，E492G+K573A，E492G+N503K+K573A，E492G+N503H，E492G+K573P，E492G+N503K+K573P，E492G+N503H+K573P。
4. method according to claim 1, wherein albumin variants according to claim 2, its fragment or the fusion polypeptide that comprises described variant albumin or its fragment have shorter plasma half-time compared with described parent's albumin.
5. method according to claim 4, wherein albumin variants according to claim 5, its fragment or the fusion polypeptide that comprises described variant albumin or its fragment comprise the one or more replacements corresponding to being selected from following replacement in SEQIDNO:2:
K500E,G,D,A,S,C,P,H,F,N,W,T,M,Y,V,Q,L,I,R，D550N，H464Q，H510Q，H535Q，D494N,Q,A，E495Q,A，T496A，E492G+V493P，K541G,D，D494N+E495Q+T496A，E495Q+T496A，E492G+V493P+K538H+K541N+E542D，N503K,DP499A，K541A,N，D494E+Q417H，Q417A，E492T+N503D，A490D+E492T+V493L+E501P+N503D+A504E+E505K+T506F+K541D，K536A，P537A，K538A，E501A,Q，E492G+K538H+K541N+E542D。
6. the method according to any one of claim 1-4, wherein comprises one or more further change at its Surface Creation methylthio group according to albumin variants in any one of the preceding claims wherein, its fragment or the fusion polypeptide that comprises described variant albumin or its fragment.
7. the method according to any one of claim 1-6, the sequence iden of the albumin variants wherein according to aforementioned claim, its fragment or the fusion polypeptide and SEQIDNO:2 that comprise described variant albumin or its fragment is greater than 80%, be preferably greater than 90%, more preferably greater than 95%, more preferably greater than 96%, even more preferably greater than 97%, be most preferably greater than 99% more preferably greater than 98%.
8. the method according to any one of claim 1-7, albumin variants wherein according to aforementioned claim, its fragment or the fusion polypeptide comprising described variant albumin or its fragment are albuminous Variants Fragments or the fusion polypeptide comprising albuminous Variants Fragments, wherein said fragment is at least 20 amino acid, preferably at least 50 amino acid, preferably at least 100 amino acid, more preferably at least 200 amino acid, more preferably at least 300 amino acid, more preferably at least 400 amino acid and most preferably at least 500 amino acid.
9. an albumin variants, its fragment or comprise the fusion polypeptide of described variant albumin or its fragment, itself and parent's albumin, its fragment or the fusion polypeptide comprising described parent's albumin or its fragment compare the plasma half-time with change, it is comprising one or more replacement corresponding to the position being selected from following position in SEQIDNO:2: 500, 550, 492, 580, 574, 417, 440, 464, 490, 493, 494, 495, 496, 499, 501, 503, 504, 505, 506, 510, 535, 536, 537, 538, 540, 541, 542, 575, 577, 578, 579, 581, 582 and 584, wherein said variant is not by having replacement D494N, E501K, K541E, D550G, the variant of the SEQIDNO:2 composition of A or K574N.
10. albumin variants according to claim 9, its fragment or comprise the fusion polypeptide of described variant albumin or its fragment, it has longer plasma half-time compared with described parent's albumin.
11. albumin variants according to claim 10, its fragment or comprise the fusion polypeptide of described variant albumin or its fragment, it comprises the one or more replacements corresponding to replacement following in SEQIDNO:2: K573P+K574N+A577T+A578R+S579C+Q580K+A581D+G584A, K573P+A577E+A578S+Q580K+A582T, K573P+A578S+S579T+G584A, K573P+L575F+G584A, E492G, N503K, K500R, N503H, D550E, K574N, Q580K, E492G+N503K, E492G+N503H, E492H+E501P+N503H+E505D+T506S+T540S+K541E, E492G+K573A, E492G+N503K+K573A, E492G+N503H, E492G+K573P, E492G+N503K+K573P, E492G+N503H+K573P.
12. albumin variants according to claim 9, its fragment or comprise the fusion polypeptide of described variant albumin or its fragment, it has shorter plasma half-time compared with described parent's albumin.
13. albumin variants according to claim 12, its fragment or comprise the fusion polypeptide of described variant albumin or its fragment, it comprises the one or more replacements corresponding to being selected from following replacement in SEQIDNO:2: K500E, G, D, A, S, C, P, H, F, N, W, T, M, Y, V, Q, L, I, R, D550N, H464Q, H510Q, H535Q, D494N, Q, A, E495Q, A, T496A, E492G+V493P, K541G, D, D494N+E495Q+T496A, E495Q+T496A, E492G+V493P+K538H+K541N+E542D, N503K, DP499A, K541A, N, D494E+Q417H, Q417A, E492T+N503D, A490D+E492T+V493L+E501P+N503D+A504E+E505K+T506F+K541D, K536A, P537A, K538A, E501A, Q, E492G+K538H+K541N+E542D.
14. albumin variants according to claim 9, its fragment or comprise the fusion polypeptide of described variant albumin or its fragment, it has the one or more replacements corresponding to being selected from following replacement in SEQIDNO:2:
Q417A,C,D,E,F,G,H,I,K,L,M,N,P,R,S,T,V,W,Y；
H440A,C,D,E,F,G,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
A490C,D,E,F,G,H,I,K,L,M,N,P,R,S,T,V,W,Y；
E492A,C,D,F,G,H,I,K,L,M,P,Q,R,S,T,V,W,Y；
V493A,C,D,E,F,G,H,I,K,L,M,N,P,Q,R,S,T,W,Y；
D494A,C,E,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
E495A,C,D,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
T496A,C,D,E,F,G,H,I,K,L,M,N,P,Q,R,S,V,W,Y；
P499A,C,D,E,F,G,H,I,K,L,M,N,Q,R,S,T,V,W,Y；
K500A,C,D,E,F,G,H,I,,L,M,N,Q,R,S,T,V,W,Y；
E501A,C,D,F,G,H,I,L,M,N,P,Q,R,S,T,V,W,Y；
N503A,C,D,E,F,G,H,I,K,L,M,P,Q,R,S,T,V,W,Y；
A504C,D,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
E505A,C,D,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
T506A,C,D,F,G,H,I,K,L,M,N,P,Q,R,S,V,W,Y；
H510A,C,D,F,G,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
H535A,C,D,F,G,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
K536A,C,D,E,F,G,H,I,L,M,N,P,Q,R,S,T,V,W,Y；
P537A,C,D,E,F,G,H,I,K,L,M,N,Q,R,S,T,V,W,Y；
K538A,C,D,E,F,G,H,I,L,M,N,P,Q,R,S,T,V,W,Y；
T540A,C,D,E,F,G,H,I,L,M,N,P,Q,R,S,,V,W,Y；
K541A,C,D,F,G,H,I,L,M,N,P,Q,R,S,T,V,W,Y；
E542A,C,D,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
D550C,E,F,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
K574A,C,D,E,F,G,H,I,L,M,P,Q,R,S,T,V,W,Y；
Q580A,C,D,E,F,G,H,I,K,L,M,N,P,R,S,T,V,W,Y；
A581C,D,E,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
A582C,D,E,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
G584A,C,D,E,F,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
H464A,C,D,E,F,G,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
L575A,C,D,E,F,G,H,I,K,M,N,P,Q,R,S,T,V,W,Y；
A577C,D,E,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y；
A578C, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, V, W, Y; Or
S579A,C,D,E,F,G,H,I,K,L,M,N,P,Q,R,T,V,W,Y；
15. the albumin variants according to any one of claim 9-14, its fragment or comprise the fusion polypeptide of described variant albumin or its fragment, it comprises one or more further change at its Surface Creation methylthio group.
16. the albumin variants according to any one of claim 9-15, its fragment or comprise the fusion polypeptide of described variant albumin or its fragment, the sequence iden of wherein said albumin variants, its fragment or the fusion polypeptide and SEQIDNO:2 that comprise described variant albumin or its fragment is greater than 80%, be preferably greater than 90%, more preferably greater than 95%, more preferably greater than 96%, even more preferably greater than 97%, be most preferably greater than 99% more preferably greater than 98%.
17. the albumin variants according to any one of claim 9-16, its fragment or comprise the fusion polypeptide of described variant albumin or its fragment, it is albuminous Variants Fragments or the fusion polypeptide comprising albuminous Variants Fragments, wherein said fragment is at least 20 amino acid, preferably at least 50 amino acid, preferably at least 100 amino acid, more preferably at least 200 amino acid, more preferably at least 300 amino acid, more preferably at least 400 amino acid and most preferably at least 500 amino acid.
18. a nucleic acid, the albumin variants of its coding according to any one of claim 9-17, its fragment or comprise the fusion polypeptide of described variant albumin or its fragment.
19. a conjugate, it comprises variant albumin according to any one of claim 9-18 or its fragment and useful treatment module.
20. an associated complex, it comprises variant albumin according to any one of claim 9-19 or its fragment and useful treatment module.
21. a fusion polypeptide, it comprises variant albumin according to any one of claim 9-10 or its fragment and fusion partner polypeptide.
22. 1 kinds of compositions, it comprises albumin variants, its fragment or comprises the fusion polypeptide of described variant albumin or its fragment or comprise the albuminous conjugate of described variant, wherein with corresponding albumin or its fragment or comprise the fusion polypeptide of described albumin or its fragment or comprise compared with described albuminous conjugate, stronger to the combination of FcRn.
23. compositions according to claim 22, wherein its to the combination of FcRn and HSA to strong compared with the combination of FcRn.
24. compositions according to claim 23, wherein said albumin variants, its fragment or comprise the fusion polypeptide of described variant albumin or its fragment or comprise the 0.9XKD that the attachment coefficient of the albuminous conjugate of described variant to FcRn be less than HSA, more preferably less than the 0.5XKD of HSA, more preferably less than the 0.1XKD of HSA, even more preferably less than the 0.05XKD of HSA, even more preferably less than HSA 0.02XKD and be most preferably less than the 0.01XKD of HSA.
25. 1 kinds of compositions, it comprises albumin variants, its fragment or comprises the fusion polypeptide of described variant albumin or its fragment or comprise the albuminous conjugate of described variant, wherein with corresponding albumin or its fragment or comprise the fusion polypeptide of described albumin or its fragment or comprise compared with described albuminous conjugate, more weak to the combination of FcRn.
26. compositions according to claim 25, wherein its to the combination of FcRn and HSA to weak compared with the combination of FcRn.
27. compositions according to any one of claim 22-26, it comprises variant albumin according to any one of claim 9-17 or its fragment, conjugate according to claim 19, associated complex according to claim 20 or fusion polypeptide according to claim 21.
28. compositions according to any one of claim 22-27, it comprises variant albumin according to any one of claim 9-17 or its fragment, also inclusion compound, described compound comprises albumin bound territory (ABD) and the upper useful module for the treatment of.
29. compositions according to any one of claim 22-29, it is pharmaceutical compositions.
30. variant albumin, fragment, fusion polypeptide or conjugates according to any one of claim 9-30, associated complex or the composition comprising described Variants Fragments or fusion polypeptide are used as the purposes of medicine.
31. variant albumin, fragment, fusion polypeptide or conjugate according to any one of claim 9-30, associated complex or the composition comprising described Variants Fragments or fusion polypeptide are manufacturing the purposes in pharmaceutical compositions.
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