CN105534724A - Resveratrol-coated nano solid lipid carrier and preparation method thereof - Google Patents

Abstract

The invention discloses a nanometer solid lipid carrier coated with resveratrol and a preparation method thereof. The nanometer solid lipid carrier coated with resveratrol consists of 0.1 to 15% resveratrol, 0.5% ～5% phospholipid, 3～30% solid emulsifier, 2～15% solid lipid, 3～18% liquid lipid, 5～20% polyol, 0.02～1% inorganic salt, 0.01～0.8% preservative and The remainder consisted of deionized water. Its preparation method is to melt and disperse the oil phase composed of resveratrol, phospholipid, solid emulsifier, solid lipid and liquid lipid evenly, and simultaneously heat the water phase composed of polyhydric alcohol, inorganic salt and deionized water, and stir The water phase is added to the oil phase, mixed, ultrasonicated, then stirred and cooled, and preservatives are added to form a nano-solid lipid carrier coated with resveratrol. Its particle size is 50-200nm, which can effectively penetrate into the stratum corneum.

Description

A nano-solid lipid carrier coated with resveratrol and its preparation method

technical field

The invention relates to a nanometer solid lipid carrier coated with resveratrol and a preparation method thereof, belonging to the field of chemical industry.

Background technique

The natural functional ingredient resveratrol, also known as stilbene triphenol, is a colorless needle-like crystal, insoluble in water, but easily soluble in organic solvents such as ethanol and acetone. Its melting point is 253-255°C, and it sublimates at 261°C. Its structural formula is As a non-flavonoid polyphenol compound, it not only has some common characteristics of plant polyphenols, but also has its unique biological activity. Studies have found that most polyphenols have significant antioxidant and anti-free radical effects.

Studies have shown that resveratrol has good whitening and anti-freckle effects when used in cosmetics. But resveratrol is insoluble in water, only easily soluble in organic solvents such as ethanol, acetone, acetone can not be directly used in cosmetics, ethanol can easily cause skin allergies. At the same time, it has poor biocompatibility and is not easy to penetrate through the skin; on the other hand, resveratrol is unstable to light and heat, and is only stable in a neutral pH environment under dark conditions. These factors limit the application of resveratrol in cosmetics.

Nano solid lipid carrier (NLC) is a solid particle formed by solidification and crystallization of solid oil during cooling or phase separation. As a new type of carrier, nano-solid lipid carrier has the advantages of good physiological compatibility, biodegradability, strong drug-loading capacity, stable physical and chemical storage, low cost, and large-scale production. Acting in cosmetics, it can enhance the permeability of active ingredients, improve the closure effect and hydration, and at the same time, as a coating carrier, it can improve the stability of active ingredients.

However, the preparation of nano-solid lipid carriers coated with resveratrol is more researched on the high-pressure homogenization method and the common emulsification method. The advantage of the high-pressure homogenization method is that the prepared nanoparticles have a small particle size, but the disadvantage is that the operation is complicated and the structure is easily damaged. The ordinary emulsification method is simple to operate, but the prepared samples will be unevenly dispersed and have large particle sizes, which is not conducive to the penetration of active ingredients.

Patent CN102614091A (published on August 1, 2012), discloses a nanostructured lipid carrier coated with resveratrol. Since the emulsifiers are mainly Tween and fatty alcohol polyoxyethylene ether series, it belongs to polyoxygen-containing The nonionic surfactant of ethylene unit, the nano-structure lipid carrier of coating resveratrol that obtains like this can bring dioxane into cosmetics because of the nonionic surfactant containing polyoxyethylene unit, and then Penetrates the skin and causes unsafe conditions.

Further, the nanoliposomes coated with resveratrol disclosed in patent CN103040754A (published on April 17, 2013), since the solvents used in the preparation process are ethanol, methanol, chloroform, ether, petroleum ether, etc., therefore, Since the resveratrol-coated nanoliposomes also contain solvents in the system, these solvents are banned and restricted raw materials in cosmetics, and the application of this coating technology in cosmetics will also be unsafe.

Patent CN101874763A (published on November 3, 2011) mainly obtains the resveratrol liposome system by thin film evaporation method and adding organic solvents. This technology also has residual solvents and cannot be directly used as a coating carrier for cosmetic raw materials. In the cosmetic product system.

In summary, the nanostructured lipid carriers coated with resveratrol in the prior art all have safety issues when directly applied to cosmetics. And the present invention just proposes in order to solve this technical problem.

Contents of the invention

One of the purposes of the present invention is to provide a nano-solid coated resveratrol with phospholipid as one of the lipid raw materials in order to solve the technical problems of above-mentioned resveratrol instability, poor permeability and low utilization rate Lipid carrier, in the nanometer solid lipid carrier coated with resveratrol, resveratrol is stable, has good permeability, and improves its utilization rate at the same time.

The second object of the present invention is to provide a method for preparing the above-mentioned nano solid lipid carrier coated with resveratrol.

Technical scheme of the present invention

A nanometer solid lipid carrier coated with resveratrol, the particle size is 50-200nm, calculated by weight percentage, its raw material composition and content are as follows:

The balance is deionized water;

Wherein, the phospholipid is one or a combination of two or more of soybean lecithin, egg yolk lecithin, and hydrogenated lecithin with a phosphatidylcholine (hereinafter referred to as PC) content of 50% to 95%; preferably phosphatidylcholine Soybean lecithin with a choline content of 50%-90%, egg yolk lecithin with a phosphatidylcholine content of 70%, or hydrogenated lecithin with a phosphatidylcholine content of 95%;

Described solid emulsifier is cetearethers, PEG esters, glucosides, polyglycerol-3 methylglucose distearate, sodium stearyl glutamate, Gemini star emulsifier or polyglyceryl-6 distearate;

Wherein the stearyl ethers are ceteareth-25, ceteareth-6, ceteareth-12, ceteareth-20, ceteareth Steareth-30, Steareth-2, Steareth-21, or Ciceteareth-4 phosphoric acid;

PEG esters are PEG-20 glyceryl tribehenate, PEG-8 dioleate, PEG-8 distearate, PEG-4 laurate, PEG-8 oleate, PEG-8 stearate Esters, PEG-8 beeswax, PEG-20 stearate, PEG-32 stearate, PEG-40 hydrogenated castor oil, PEG-20 stearyl ether, PEG-15 cetostearyl ether, PEG- 2 cetyl alcohol ether, PEG-10 cetyl alcohol ether or PEG-4 laurate;

Glucosides are Cetearyl Glucoside, Hydroxystearyl Alcohol and Hydroxystearyl Glucoside, Sucrose Esters, Arachidyl Alcohol Glucoside, Behenyl Alcohol Glucoside, Cetearyl Glucoside, Cetearyl Alcohol Glucoside Glucoside, coco glucoside, PEG-20 methyl glucoside sesquistearate or methyl glucoside sesquistearate;

The solid lipids are saturated fatty acids, steroids, glycerides, waxes, alcohols, hydrogenated castor oil, cetearyl alcohol, stearyl alcohol or pentaerythritol distearate;

Wherein the saturated fatty acid is stearic acid, palmitic acid, capric acid or behenic acid;

Steroids are cholesterol;

The glyceryl esters are glyceryl stearate, cetearyl caprylate, glyceryl monostearate, cetyl palmitate, glyceryl tristearate or glyceryl trilaurate;

Waxes are pentaerythritol tetrabehenate, pentaerythritol tetracaprylate/caprate, pentaerythritol tetraoleate, pentaerythritol tetrastearate, myristyl myristate, cetyl lactate, cetyl ricinoleate esters, myristyl myristate, cetyl palmitate, stearyl stearyl, cetyl palmitate or dipentaerythritol hexacaprylic/capric acid;

The alcohols are cetyl alcohol, stearyl alcohol, behenyl alcohol or cetostearyl alcohol;

The liquid lipid is one or a combination of two or more of vegetable oil, mineral oil and synthetic oil;

The vegetable oil is jojoba oil, macadamia nut oil, olive oil, sweet almond oil, grapeseed oil, avocado oil, rice bran oil, safflower oil, coconut oil, castor oil, sunflower seed oil, wheat germ oil or lavender oil Wait;

Mineral grease is white oil;

Synthetic oils are isopropyl myristate, isopropyl isostearate, tridecyl stearate, glyceryl stearate, isononyl isononanoate, iso-hexadecane, isooctyl palmitate, Isopropyl palmitate, caprylic/capric triglycerides, myristyl myristate, squalane, ethyl oleate, tridecyl stearate or isooctyl isostearate;

The polyhydric alcohol is one or a combination of two or more of glycerin, sorbitol, propylene glycol and 1,3-butanediol;

The inorganic salt is one or a mixture of two or more of sodium chloride, potassium chloride or calcium chloride;

The preservatives are benzoic acids, sorbic acids, paraben esters, genmas, carsons, dimethylol hydantoin, 1,2-benzisothiazolin-3-one, benzoic acid Methyl formate, methylisothiazolinone, trichlorodiphenyl urea, 2-bromo-2-nitro-1.3-propanediol (Bropol), glyceryl monolaurate, diazolidinyl urea, propylene glycol -5-Bromo-5-nitro-1,3-dioxane, dehydroacetic acid, propylene glycol-5-bromo-5-nitro-1,3-dioxane, propylene glycol-bis-imidazolidinyl urea, bis-imidazolidine One or a combination of two or more of urea/methylparaben/propylene glycol, iodopropynyl butyl carbamate;

Described parabens are preferably methyl parabens;

Gemmas are imidazolidinyl urea and bis-imidazolidinyl urea.

The above-mentioned preparation method of a nano-solid lipid carrier coated with resveratrol comprises the following steps:

(1) Add phospholipids, resveratrol, solid lipids, liquid lipids, and solid emulsifiers into the container, control the temperature to melt at 85-100°C, and disperse evenly to obtain an oil phase;

(2) Add polyols, inorganic salts and deionized water into another container, heat to 85-100°C, and homogenize at 800rpm to obtain a water phase;

(3) Add the oil phase obtained in step (1) into the homogeneous water phase in step (2), and continue homogenizing at a rate of 800 rpm for 3 to 6 minutes to obtain an emulsified system;

(4) Ultrasonicate the emulsified system obtained in step (3) for 4-20min with a power of 80-800W, then cool while stirring at a speed of 200-800r/min, when the temperature drops to 40-60°C, add a preservative and Stir evenly, and then cool to room temperature to obtain a nanometer solid lipid carrier coated with resveratrol.

Beneficial effects of the present invention:

A kind of nano-solid lipid carrier coated with resveratrol of the present invention, because resveratrol is coated in the nano-solid lipid carrier structure, has avoided the direct irradiation of light, therefore has improved the resveratrol stability;

Further, a kind of nano-solid lipid carrier coated resveratrol of the present invention, owing to contain phospholipid in this nano-solid lipid carrier structure, the similarity of phospholipid structure and skin lipid structure has improved coating resveratrol Adhesion of the resveratrol nano-solid lipid carrier structure on the skin, thus, promoting the permeability of resveratrol;

Further, because resveratrol is coated in the nano-solid lipid carrier structure to form an aqueous suspension, it is beneficial to disperse in the aqueous medium, and resveratrol exists in the carrier structure with molecular structure; at the same time, the carrier structure promotes Penetration of active ingredients and therefore high availability of resveratrol.

Further, a kind of nano-solid lipid carrier coated with resveratrol of the present invention, owing to adopted liposome main raw material phospholipid to be applied in solid lipid carrier structure, on the basis of solid lipid carrier structure advantage, increase It not only improves the characteristics of good affinity between liposome and skin, but also avoids the disadvantages of single structure of liposome and solid lipid carrier, such as liposome structure is easily damaged by surfactant, and solid lipid particle carrier is With the deficiencies in aspects such as insufficient skin affinity, in combination with the preparation method of the nano-solid lipid carrier coated with resveratrol of the present invention, the particle diameter of the nano-solid liposome coated with resveratrol can be effectively controlled. 50-200nm, which is more conducive to its adhesion to the stratum corneum, and promotes the penetration of the active ingredient resveratrol on the stratum corneum, thereby improving the utilization rate of resveratrol.

Description of drawings

Fig. 1, the scanning electron micrograph of the nano-solid lipid carrier coated with resveratrol obtained in Example 6.

detailed description

The present invention will be further described below in conjunction with specific examples, but the protection scope of the present invention is not limited thereto.

The raw materials used in each embodiment of the present invention, such as solid lipids, liquid lipids, resveratrol, phospholipids, solid emulsifiers, polyols, inorganic salts, etc., are common raw materials in cosmetics unless otherwise specified. , is commercially available.

The nano-solid lipid carrier coated with resveratrol containing phospholipid obtained in Example 3 of the present invention is carried out with the obtained nano-solid lipid carrier coated resveratrol without phospholipid obtained in the comparative example of embodiment 3 Resveratrol permeability data.

The determination method is as follows:

1. Instruments used: Agilent1260 high performance liquid chromatography, HS-02 horizontal transdermal diffusion and penetration instrument.

2. Operation steps:

(1) Prepare receiving solution: 40% PBS buffer solution + 60% absolute ethanol.

(2) In vitro transdermal diffusion test

①Use a 2mL pipette to pipette 2mL of the phospholipid-containing resveratrol-coated nano-solid lipid carrier obtained in Example 3 to be tested into a 10mL volumetric flask, dilute to 10mL with deionized water, shake evenly, Dilute the original sample to 0.8% resveratrol nano-lipid carrier, stock solution 1; simultaneously pipette 2mL of the obtained phospholipid-free nano-solid lipid carrier coated with resveratrol in the comparative example of Example 3 , do the same treatment, dilute to 10mL with deionized water, shake well, spare solution 2;

②Put a stirrer in the receiving tank and fill it with receiving solution, fix the artificial skin between the supply tank and the receiving tank of the diffusion tank (the bright side faces the supply tank, and the dark side faces the receiving tank), so that the skin is immersed in the solution;

③ Fill the supply tank with the reserve solution 1 in (1) as the supply solution, and fill the other supply tank with the reserve solution 2 as the supply solution, and compare the permeability of the two samples at the same time;

④Keep the temperature of the circulating water at 37°C, draw 1mL of the receiving solution with a 1mL medical sterile syringe at a certain speed at 1h, 2h, 4h, 6h, 8h, 10h, 12h, 24h, and 36h, and replenish the same volume of fresh receiver solution. After the sample was filtered through a 0.22 μm filter membrane, the concentration of resveratrol in the filtrate was measured by high performance liquid chromatography.

$\mathrm{<span\; class="notranslate">\; Q</span>}<span\; class="notranslate">\; =</span><span\; class="notranslate">\; \&lsqb;</span>{\mathrm{<span\; class="notranslate">\; C</span>}}_{\mathrm{<span\; class="notranslate">\; no</span>}}<span\; class="notranslate">\; \&times;</span>\mathrm{<span\; class="notranslate">\; V</span>}<span\; class="notranslate">\; +</span>\underset{\mathrm{<span\; class="notranslate">\; i</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{\overset{\mathrm{<span\; class="notranslate">\; no</span>}<span\; class="notranslate">\; -</span>\mathrm{<span\; class="notranslate">\; 1</span>}}{<span\; class="notranslate">\; \&Sigma;</span>}}{\mathrm{<span\; class="notranslate">\; C</span>}}_{\mathrm{<span\; class="notranslate">\; i</span>}}<span\; class="notranslate">\; \&times;</span>\mathrm{<span\; class="notranslate">\; 1.0</span>}<span\; class="notranslate">\; \&rsqb;</span><span\; class="notranslate">\; /</span>\mathrm{<span\; class="notranslate">\; S</span>}$

Experimental results:

Where C _n : drug concentration in the receiving solution at the nth sampling, μg/mL;

V: the volume of receiving liquid in the receiving chamber, 7mL;

C _i : drug concentration in the receiving solution at the i-th sampling, μg/mL;

1.0: Each sampling volume, mL;

S: effective permeable area, 1cm ² ;

Q: Permeability, μg/cm ² .

Example 1

A nano-solid lipid carrier coated with resveratrol, calculated according to the total amount of 100g, its raw material composition and content are as follows:

The balance is deionized water;

The phospholipid is soybean lecithin with a PC content of 50%, a food-grade product produced by Beijing Meas Phospholipid Technology Co., Ltd.;

Described solid emulsifier is ceteareth-20;

Described solid lipid is glyceryl monostearate;

The liquid lipid is macadamia nut oil;

Described polyhydric alcohol is propylene glycol;

Described inorganic salt is potassium chloride;

The preservative is diazolidinyl urea.

The nano-solid lipid carrier coated with resveratrol is characterized in that it specifically includes the following steps:

(1) Add phospholipids, resveratrol, solid lipids, liquid lipids, and solid emulsifiers into the container, control the temperature to melt at 85-100°C, and disperse evenly to obtain an oil phase;

(2) Add polyols, inorganic salts and deionized water into another container, heat to 85-100°C, and homogenize at 800rpm to obtain a water phase;

(3) Add the oil phase obtained in step (1) into the homogeneous water phase in step (2), and continue homogenizing at a rate of 800 rpm for 3 to 6 minutes to obtain an emulsified system;

(4) Ultrasonicate the emulsified system obtained in step (3) for 4-20min with a power of 80-800W, then cool while stirring at a speed of 200-800r/min, when the temperature drops to 40-60°C, add a preservative and Stir evenly, and then cool to room temperature to obtain a nanometer solid lipid carrier coated with resveratrol.

Example 2

A nano-solid lipid carrier coated with resveratrol, calculated according to the total amount of 100g, its raw material composition and content are as follows:

The balance is deionized water;

The phospholipid is egg yolk lecithin with a PC content of 70%, a food-grade product produced by Beijing Meas Phospholipid Technology Co., Ltd.;

Described solid emulsifier is PEG-8 dioleate;

The solid lipid is cetyl alcohol;

Described liquid lipid is tridecyl stearate;

Described polyhydric alcohol is glycerol;

Described inorganic salt is sodium chloride;

Described preservative is methylparaben.

The preparation method of the nano-solid lipid carrier coated with resveratrol is the same as that in Example 1.

Example 3

A nano-solid lipid carrier coated with resveratrol, that is, a nano-solid lipid carrier coated with resveratrol containing phospholipids, calculated according to the total amount of 100g, its raw material composition and content are as follows:

The balance is deionized water;

The phospholipid is soybean lecithin with a PC content of 70%, a food-grade product produced by Shanxi Senfu Natural Products Co., Ltd.;

Described solid emulsifier is cetearyl glucoside;

Described solid lipid is hydrogenated castor oil;

Described liquid lipid is isopropyl palmitate;

Described polyhydric alcohol is 1,3-butanediol;

Described inorganic salt is calcium chloride;

The preservative is propylene glycol-bis-imidazolidinyl urea.

The preparation method of the resveratrol-coated nano-solid lipid carrier, that is, the phospholipid-coated resveratrol-coated nano-solid lipid carrier is the same as in Example 1.

Comparative example of embodiment 3

A nano-solid lipid carrier coated with resveratrol, calculated according to the total amount of 100g, its raw material composition and content are as follows:

The balance is deionized water;

Described solid emulsifier is cetearyl glucoside;

Described solid lipid is hydrogenated castor oil;

Described liquid lipid is isopropyl palmitate;

Described polyhydric alcohol is 1,3-butanediol;

Described inorganic salt is calcium chloride;

The preservative is propylene glycol-bis-imidazolidinyl urea.

The nano-solid lipid carrier coated with resveratrol specifically includes the following steps:

(1) Add resveratrol, solid lipid, liquid lipid, and solid emulsifier into the container, control the temperature to melt at 85-100°C, and disperse evenly to obtain an oil phase;

(2) Add polyols, inorganic salts and deionized water into another container, heat to 85-100°C, and homogenize at 800rpm to obtain a water phase;

(3) Add the oil phase obtained in step (1) into the homogeneous water phase in step (2), and continue homogenizing at a rate of 800 rpm for 3 to 6 minutes to obtain an emulsified system;

(4) Ultrasonicate the emulsified system obtained in step (3) for 4-20min with a power of 80-800W, then cool while stirring at a speed of 200-800r/min, when the temperature drops to 40-60°C, add a preservative and Stir evenly, and then cool to room temperature to obtain a resveratrol-coated nanometer solid lipid carrier without phospholipids.

In the nano-solid lipid carrier of the coated resveratrol containing phospholipid obtained in the above-mentioned embodiment 3 and the obtained nano-solid lipid carrier of the coated resveratrol not containing phospholipid obtained in the comparative example of embodiment 3 Resveratrol permeability data determination, the results are shown in the following table:

It can be seen from the above table that the adhesion amount of the resveratrol coated in the phospholipid-containing nano-solid lipid carrier structure coated with resveratrol obtained in Example 3 on the artificial skin is greater than that of the comparison of Example 3 The adhesion amount of resveratrol coated in the solid lipid carrier structure without phospholipid obtained in the embodiment, the high adhesion amount is conducive to promoting the penetration of resveratrol in the stratum corneum, thus indicating that the content of The resveratrol encapsulated in the solid lipid carrier structure of phospholipids is more conducive to the penetration on the skin.

Example 4

A nano-solid lipid carrier coated with resveratrol, calculated according to the total amount of 100g, its raw material composition and content are as follows:

The balance is deionized water;

The phospholipid is soybean lecithin with a PC content of 80%, a food-grade product produced by Shanxi Senfu Natural Products Co., Ltd.;

Described solid emulsifier is polyglyceryl-6 distearate;

The solid lipid is behenic acid;

Described liquid lipid is wheat germ oil;

Described polyhydric alcohol is propylene glycol;

Described inorganic salt is potassium chloride;

The preservative is 2-bromo-2-nitro-1.3-propanediol.

The preparation method of the above-mentioned nano-solid lipid carrier coated with resveratrol is the same as that in Example 1.

Example 5

A nano-solid lipid carrier coated with resveratrol, calculated according to the total amount of 100g, its raw material composition and content are as follows:

The balance is deionized water

The phospholipid is soybean lecithin with a PC content of 90%, a pharmaceutical grade product produced by Shanxi Senfu Natural Products Co., Ltd.;

Described solid emulsifier is sucrose ester;

The solid lipid is palmitic acid;

Described liquid lipid is isopropyl myristate;

Described polyhydric alcohol is sorbitol;

Described inorganic salt is potassium chloride;

Described preservative is methylparaben.

The preparation method of the above-mentioned nano-solid lipid carrier coated with resveratrol is the same as that in Example 1.

Example 6

A nano-solid lipid carrier coated with resveratrol, calculated according to the total amount of 100g, its raw material composition and content are as follows:

The balance is deionized water;

The phospholipid is a hydrogenated lecithin with a PC content of 95%, a pharmaceutical-grade product provided by Shanghai Yiji Biotechnology Co., Ltd.;

Described solid emulsifier is Gemini emulsifier;

The solid lipid is dipentaerythritol hexacaprylic acid/caprate;

The liquid lipid is caprylic acid/capric triglyceride;

Described polyhydric alcohol is 1,3-butanediol;

Described inorganic salt is sodium chloride;

The preservative is diazolidinyl urea.

The preparation method of the above-mentioned nano-solid lipid carrier coated with resveratrol is the same as that in Example 1.

Using a Quanta200F scanning electron microscope (FEIcompany, Quanta200FEG, Holland, accelerating voltage 5 ~ 30kv) to scan the appearance of the nano-solid lipid carrier coated with resveratrol obtained above, the resulting microscopic appearance is shown in Figure 1 Show. It can be seen from Figure 1 that the nano-solid lipid particles are closely arranged and uniform, and have a good coating effect on resveratrol.

In summary, a nano-solid lipid carrier coated with resveratrol of the present invention improves the adhesion of the carrier structure on the skin due to the introduction of phospholipids into the carrier structure, thereby benefiting the resveratrol Penetration on the skin.

The foregoing is only an example of the embodiment of the present invention. It should be pointed out that for those of ordinary skill in the art, some improvements and modifications can be made without departing from the technical principles of the present invention. These improvements and Modifications should also be regarded as the scope of protection of the present invention.

Claims (9)

1. a nano-solid lipid carrier coated with resveratrol, characterized in that the nano-solid lipid carrier coated with resveratrol has a particle diameter of 50 to 200nm, calculated by weight percentage, its The composition and content of raw materials are as follows: The phospholipid is one or more combinations of soybean lecithin, egg yolk lecithin and hydrogenated lecithin with a phosphatidylcholine content of 50% to 95%; Described solid emulsifier is cetearethers, PEG esters, glucosides, polyglycerol-3 methylglucose distearate, sodium stearyl glutamate, Gemini star emulsifier or polyglycerol 6-distearate; Wherein the stearyl ethers are ceteareth-25, ceteareth-6, ceteareth-12, ceteareth-20, ceteareth Steareth-30, Steareth-2, Steareth-21, or Ciceteareth-4 phosphoric acid; PEG esters are PEG-20 glyceryl tribehenate, PEG-8 dioleate, PEG-8 distearate, PEG-4 laurate, PEG-8 oleate, PEG-8 stearate Esters, PEG-8 beeswax, PEG-20 stearate, PEG-32 stearate, PEG-40 hydrogenated castor oil, PEG-20 stearyl ether, PEG-15 cetostearyl ether, PEG- 2 cetyl alcohol ether, PEG-10 cetyl alcohol ether or PEG-4 laurate; Glucosides are Cetearyl Glucoside, Hydroxystearyl Alcohol and Hydroxystearyl Glucoside, Sucrose Esters, Arachidyl Alcohol Glucoside, Behenyl Alcohol Glucoside, Cetearyl Glucoside, Cetearyl Alcohol Glucoside Glucoside, coco glucoside, PEG-20 methyl glucoside sesquistearate or methyl glucoside sesquistearate; The solid lipids are saturated fatty acids, steroids, glycerides, waxes, alcohols, hydrogenated castor oil, cetearyl alcohol, stearyl alcohol or pentaerythritol distearate; Wherein the saturated fatty acid is stearic acid, palmitic acid, capric acid or behenic acid; Steroids are cholesterol; The glyceryl esters are glyceryl stearate, cetearyl caprylate, glyceryl monostearate, cetyl palmitate, glyceryl tristearate or glyceryl trilaurate; Waxes are pentaerythritol tetrabehenate, pentaerythritol tetracaprylate/caprate, pentaerythritol tetraoleate, pentaerythritol tetrastearate, myristyl myristate, cetyl lactate, cetyl ricinoleate esters, myristyl myristate, cetyl palmitate, stearyl stearyl, cetyl palmitate or dipentaerythritol hexacaprylic/capric acid; The alcohols are cetyl alcohol, stearyl alcohol, behenyl alcohol or cetostearyl alcohol; The liquid lipid is one or a combination of two or more of vegetable oil, mineral oil and synthetic oil; The vegetable oil is jojoba oil, macadamia nut oil, olive oil, sweet almond oil, grapeseed oil, avocado oil, rice bran oil, safflower oil, coconut oil, castor oil, sunflower seed oil, wheat germ oil or lavender oil ; Mineral grease is white oil; Synthetic oils are isopropyl myristate, isopropyl isostearate, tridecyl stearate, glyceryl stearate, isononyl isononanoate, iso-hexadecane, isooctyl palmitate, Isopropyl palmitate, caprylic/capric triglycerides, myristyl myristate, squalane, ethyl oleate, tridecyl stearate or isooctyl isostearate; The polyhydric alcohol is one or a combination of two or more of glycerin, sorbitol, propylene glycol and 1,3-butanediol; The inorganic salt is one or a mixture of two or more of sodium chloride, potassium chloride and calcium chloride; The preservatives are benzoic acids, sorbic acids, paraben esters, genmas, carsons, dimethylol hydantoin, 1,2-benzisothiazolin-3-one, benzoic acid Methyl formate, methylisothiazolinone, trichlorodiphenyl urea, 2-bromo-2-nitro-1.3-propanediol (Bropol), glyceryl monolaurate, diazolidinyl urea, propylene glycol -5-Bromo-5-nitro-1,3-dioxane, dehydroacetic acid, propylene glycol-5-bromo-5-nitro-1,3-dioxane, propylene glycol-bis-imidazolidinyl urea, bis-imidazolidine One or a combination of two or more of urea/methylparaben/propylene glycol, iodopropynyl butyl carbamate; Wherein the parabens are methyl parabens; Gemmas are imidazolidinyl urea or bis-imidazolidinyl urea. 2. a kind of nanometer solid lipid carrier of coated resveratrol as claimed in claim 1, is characterized in that: The phospholipids are soybean lecithin with a phosphatidylcholine content of 50%-90%, egg yolk lecithin with a phosphatidylcholine content of 70%, or hydrogenated lecithin with a phosphatidylcholine content of 95%; Described solid emulsifier is cetearethers, PEG esters, glucosides, Gemini emulsifier or polyglyceryl-6 distearate; Wherein the stearyl ethers are ceteareth-20; PEG esters are PEG-8 dioleate; Glucosides are cetearyl glucoside or sucrose esters; The solid lipids are saturated fatty acids, glycerides, waxes, alcohols or hydrogenated castor oil; Among them, the saturated fatty acids are palmitic acid or behenic acid; Glycerides are glyceryl monostearate; The waxy type is dipentaerythritol hexacaprylic acid/caprate; The alcohol is cetyl alcohol; Described liquid lipid is vegetable fat or synthetic fat; Wherein the vegetable oil is macadamia nut oil or wheat germ oil; Synthetic oils are isopropyl myristate, tridecyl stearate, isopropyl palmitate or caprylic/capric triglycerides; The preservatives are parabens, genmas, 2-bromo-2-nitro-1.3-propanediol, diazolidinyl urea or propylene glycol-bis-imidazolidinyl urea; Wherein the parabens are methyl parabens; Gemmas are diazolidinyl ureas. 3. a kind of nanometer solid lipid carrier of coated resveratrol as claimed in claim 2 is characterized in that by weight percentage, its raw material composition and content are as follows: The phospholipid is soybean lecithin whose phosphatidylcholine content is 50%; Described solid emulsifier is ceteareth-20; Described solid lipid is glyceryl monostearate; The liquid lipid is macadamia nut oil; Described polyhydric alcohol is propylene glycol; Described inorganic salt is potassium chloride; The preservative is diazolidinyl urea. 4. a kind of nanometer solid lipid carrier of coated resveratrol as claimed in claim 2 is characterized in that by weight percentage, its raw material composition and content are as follows: The phospholipid is egg yolk lecithin with a phosphatidylcholine content of 70%; Described solid emulsifier is PEG-8 dioleate; The solid lipid is cetyl alcohol; Described liquid lipid is tridecyl stearate; Described polyhydric alcohol is glycerol; Described inorganic salt is sodium chloride; Described preservative is methylparaben. 5. a kind of nanometer solid lipid carrier of coated resveratrol as claimed in claim 2 is characterized in that by weight percentage, its raw material composition and content are as follows: The phospholipid is soybean lecithin whose phosphatidylcholine content is 70%; Described solid emulsifier is cetearyl glucoside; Described solid lipid is hydrogenated castor oil; Described liquid lipid is isopropyl palmitate; Described polyhydric alcohol is 1,3-butanediol; Described inorganic salt is calcium chloride; The preservative is propylene glycol-bis-imidazolidinyl urea. 6. a kind of nanometer solid lipid carrier of coating resveratrol as claimed in claim 2 is characterized in that by weight percentage, its raw material composition and content are as follows: The phospholipid is soybean lecithin whose phosphatidylcholine content is 80%; Described solid emulsifier is polyglyceryl-6 distearate; The solid lipid is behenic acid; Described liquid lipid is wheat germ oil; Described polyhydric alcohol is propylene glycol; Described inorganic salt is potassium chloride; The preservative is 2-bromo-2-nitro-1.3-propanediol. 7. a kind of nanometer solid lipid carrier of coated resveratrol as claimed in claim 2 is characterized in that by weight percentage, its raw material composition and content are as follows: The phospholipid is soybean lecithin whose phosphatidylcholine content is 90%; Described solid emulsifier is sucrose ester; The solid lipid is palmitic acid; Described liquid lipid is isopropyl myristate; Described polyhydric alcohol is sorbitol; Described inorganic salt is potassium chloride; Described preservative is methylparaben. 8. a kind of nanometer solid lipid carrier of coated resveratrol as claimed in claim 2 is characterized in that by weight percentage, its raw material composition and content are as follows: The phospholipid is hydrogenated lecithin with a phosphatidylcholine content of 95%; Described solid emulsifier is Gemini emulsifier; The solid lipid is dipentaerythritol hexacaprylic acid/caprate; The liquid lipid is caprylic acid/capric triglyceride; Described polyhydric alcohol is 1,3-butanediol; Described inorganic salt is sodium chloride; The preservative is diazolidinyl urea. 9. a kind of preparation method of the nano-solid lipid carrier of coating resveratrol as described in any one of claim 1-8, it is characterized in that specifically comprising the steps: (1) Add phospholipids, resveratrol, solid lipids, liquid lipids, and solid emulsifiers into the container, control the temperature to melt at 85-100°C, and disperse evenly to obtain an oil phase; (2) Add polyols, inorganic salts and deionized water into another container, heat to 85-100°C, and homogenize at 800rpm to obtain a water phase; (3) Add the oil phase obtained in step (1) into the homogeneous water phase in step (2), and continue homogenizing at a rate of 800 rpm for 3 to 6 minutes to obtain an emulsified system; (4) Ultrasonicate the emulsified system obtained in step (3) for 4-20min with a power of 80-800W, then cool while stirring at a speed of 200-800r/min, when the temperature drops to 40-60°C, add a preservative and Stir evenly, and then cool to room temperature to obtain a nanometer solid lipid carrier coated with resveratrol.