CN105381159B - A compound aloe vera polysaccharide external gel for treating burns - Google Patents

Abstract

The invention discloses a compound aloe vera polysaccharide external gel for treating burns, which is prepared by mixing the following raw material components in parts by weight. 3-50 parts, 10-90 parts of moisturizing agent, 1-5 parts of triethanolamine, and phosphate buffer to make up to 1,000 parts; the main drug is aloe vera extract, or aloe vera curacao extract One or a combination of nano-silver powder and nano-zinc powder. The invention has the advantages of green and natural, safe and harmless, obvious effect on treating burns, fast absorption, stable and lasting drug effect, convenient carrying and transportation, etc., which not only improves the utilization rate of Chinese medicinal material resources, satisfies the medical needs, but also achieves the expansion of its purpose of clinical application.

Description

A compound aloe vera polysaccharide external gel for treating burns

technical field

The invention relates to a medical gel, in particular to a compound aloe vera polysaccharide external gel for treating burns.

Background technique

Burn generally refers to the damage to the body surface tissue, mainly skin damage, caused by heat such as boiling liquid (water, oil, soup), hot metal (liquid or solid), flame, steam and high temperature gas. In severe cases, subcutaneous tissue, muscles, bones, joints, nerves, blood vessels, and even internal organs can be injured. In the treatment of burns, the wound treatment is the main part of the treatment of burns, and local antibiotics are an important method to avoid invasive infection of the wounds. The topical antibacterial drug should have the characteristics of convenient use, broad antibacterial spectrum, few side effects, and difficulty in producing drug resistance.

The most commonly used drugs in the current treatment process are western medicines, and antibiotics are currently used more. Since the wound is the entry point for invasive infection and the local blood vessels of the burn wound are embolized, it is difficult for systemic antibiotics to reach the local area. At the same time, the widespread use of antibiotics in recent years has made the common bacteria of burns and scald infections continue to change. In addition to the differences in the use of drugs in different regions, the sensitivity of pathogenic bacteria to drugs is also different. Therefore, it is urgent to find and develop new and efficient burn drugs. question.

Silver sulfadiazine ointment is a traditional medication for treating burn wounds, with antibacterial and growth-promoting effects. However, silver sulfadiazine can cause embolism of small blood vessels and early capillaries in the surrounding tissues of the burn, which aggravates the severity of the wound to a certain extent, and has certain irritation to the burn wound.

Aloe vera is a perennial evergreen and succulent herb of the Liliaceae family. It has the characteristics of strong vitality and high medicinal value. It is an important economic plant that integrates medicine, food, beauty and ornamental. It is cold in nature and bitter in taste. It belongs to the liver, stomach and large intestine meridians. It has the functions of laxative, clearing the liver and purging fire, and killing insects to treat chancre. . Aloe vera has gradually attracted worldwide attention for its unique efficacy and wide range of uses.

There are more than 70 components in aloe vera, among which aloe vera polysaccharide is the main biologically active component of aloe vera gel. Modern medical research shows that aloe vera has anti-infection and anti-inflammatory, improving immunity, anti-tumor, anti-stomach ulcer, protecting liver, protecting myocardial ischemia, reducing blood lipid and blood sugar and other effects. Aloe vera has bacteriostatic effect. Xue Chunlan et al. have studied the bacteriostatic effect of compound aloe vera film. The bacteriostatic effect of cocci, Proteus vulgaris and Pseudomonas aeruginosa was not obvious.

Gels refer to semi-solid or thick liquid preparations of extracts and suitable bases with gel properties. According to different substrates, gels can be divided into water-based gels and oil-based gels. The aqueous gel base is generally composed of water, glycerol or propylene glycol and cellulose derivatives, carbomer and alginate, tragacanth, gelatin, starch, etc.; the oily gel base is composed of liquid paraffin and polyoxyethylene or fatty oil It is composed of colloidal silicon or aluminum soap and zinc soap. Additives such as moisturizing agents, preservatives, antioxidants, and skin penetration enhancers may be added when necessary.

SUMMARY OF THE INVENTION

The object of the present invention is to provide a compound aloe vera polysaccharide external gel for treating burns, which has the advantages of being green and natural, safe and harmless, having remarkable effect on treating burns, fast absorption, stable and lasting drug effect, convenient carrying and transportation, etc. , which not only improves the utilization rate of Chinese herbal medicine resources, meets the medical needs, but also achieves the purpose of expanding its clinical application.

The technical scheme adopted by the present invention to solve its technical problems is:

A compound aloe vera polysaccharide external gel for treating burns is prepared by mixing the following raw material components in parts by weight, and 1000 parts by weight of the raw materials are composed of: 20-80 parts of main medicine and 3-50 parts of matrix , 10-90 parts of moisturizing agent, 1-5 parts of triethanolamine, phosphate buffer to make up to 1000 parts; the main medicine is aloe vera extract, or aloe vera curacao extract and nano silver powder , one or a combination of nano-zinc powder.

The gelling agent of the present invention is a macromolecular network system with semi-solid properties, which can closely adhere to the action site for a long time, has good biocompatibility, is simple in preparation and easy to operate. The hydrated gel layer formed after the gel absorbs water and swells has a certain controlled release effect on the drug. Using the gel to treat burns can prolong the action time of the drug and the skin, and control the release rate, thereby reducing the number of administrations. , increase patient compliance. Therefore, the gel is a very suitable external preparation for burn wounds. By adopting the above formula of the present invention, the treatment effect of burns is remarkable, the absorption is fast, and the drug effect is stable and lasting. Nano-silver powder is antibacterial, and nano-zinc powder can promote wound healing.

Preferably, the matrix is selected from carbomer-940, carbomer-934, carbomer-980, hydroxypropyl methylcellulose-K4M, hydroxypropyl methylcellulose-K15M, hydroxypropyl methylcellulose Cellulose-K100M, polyethylene glycol-4000, sodium carboxymethyl cellulose, methyl cellulose, tragacanth, gelatin, polyvinylpyrrolidone, polycarbophil, alginate, sodium polyacrylate, starch , one or more of chitosan.

Preferably, it is prepared by mixing the following raw material components in parts by weight, and 1000 parts by weight of raw materials are composed of: 20 parts of aloe vera extract, 10 parts of nano silver powder, 10 parts of nano zinc powder, 5 parts of carbomer-980 parts, 50 parts of humectant, 2 parts of triethanolamine, and phosphate buffer to make up to 1000 parts. Preferably, the Aloe vera extract is Aloe vera polysaccharide, or a mixture of Aloe vera polysaccharide and Aloe vera alcohol extract in a weight ratio of 20-30:1.

Aloe vera extract is a single polysaccharide of aloe vera, which also has a good effect on the repair of burn wounds. More preferably, Aloe vera polysaccharide and Aloe vera alcohol extract are combined in a specific proportion to exert their effects, and the alcohol extract of Aloe vera contains aloe vera, aloe-emodin and other components, which are effective in the repair of burn wounds. It has a very good effect. It is used in combination with Aloe vera polysaccharide, and it works synergistically, and the effect is obviously better than that of single Aloe vera polysaccharide.

As preferably, the polysaccharide of Aloe vera is prepared by the following method:

(1) Crude polysaccharide extraction: fresh six-year-old Aloe vera, washed, de-thorn, soaked in deionized water to remove the yellow juice exuded from the epidermis, peeled, scraped the inner gel, weighed 1000g of the gel, Squeeze the juice to obtain a gel liquid, centrifuge the gel liquid, collect the supernatant, concentrate under reduced pressure, add absolute ethanol to a final ethanol concentration of 80%, stand at 4°C for 12 hours, collect the precipitate, and wash the precipitate with absolute ethanol, Vacuum drying at 60°C, and pulverizing to obtain crude polysaccharide;

(2) Deproteinization: the crude polysaccharide obtained in step (1) is dissolved in deionized water to obtain a crude polysaccharide extract. The crude polysaccharide extract and the deproteinized solution are mixed in a volume ratio of 1:0.2-0.3, centrifuged, and the supernatant is collected. liquid, repeat the above-mentioned operation 3 times, the obtained supernatant is concentrated under reduced pressure to remove the organic solvent, and after freeze-drying, aloe vera polysaccharide coarse powder is obtained;

(3) Ultrafiltration: Aloe vera polysaccharide coarse powder is dissolved in deionized water, centrifuged, and the supernatant is diluted with deionized water to obtain a solution of aloe vera polysaccharide with a concentration of 0.5 mg/mL. The solution is intercepted by an ultrafiltration membrane, and a product with a molecular weight greater than 100,000 is obtained by intercepting, and the aloe vera polysaccharide is obtained after vacuum drying.

The aloe vera polysaccharide obtained by the method of the invention, especially the product with a molecular weight greater than 100,000, has high purity and good effect on treating burns.

Preferably, the deproteinized solution is obtained by mixing chloroform and n-butanol in a volume ratio of 3:1.

Preferably, the alcohol extract of Aloe vera is prepared by the following method: freeze-drying Aloe vera, and then pulverize it at a low temperature of 2-5°C in a low-temperature ultrafine pulverizer to obtain a fineness of 100-150 Objective Aloe vera low-temperature powder, aloe vera low-temperature powder and 95% mass concentration ethanol solution are mixed uniformly according to the material-liquid ratio of 1g: 10-15ml, ultrasonic extraction is performed under the ultrasonic power of 450-500w for 30-50min, and then heated to 50-60 ℃ , extracted for 1-2h, suction filtered, evaporated and concentrated to a viscous state on a rotary evaporator, and freeze-dried. The alcohol extract of Aloe vera obtained by the specific extraction method of the present invention contains aloe vera, aloe-emodin and other components, and has a good effect on the repair of burn wounds.

Preferably, the moisturizing agent is selected from one or more of glycerin, propylene glycol, sorbitol and polyethylene glycol.

Preferably, the pH of the phosphate buffer is 6.86.

The beneficial effects of the invention are as follows: it has the advantages of being green and natural, safe and harmless, obvious in treating burns, fast in absorption, stable and lasting in drug effect, convenient in carrying and transportation, etc., which not only improves the utilization rate of Chinese medicinal material resources, but also meets the medical needs. It has achieved the purpose of expanding its clinical application and has broad application prospects.

Detailed ways

The technical solutions of the present invention will be further described in detail below through specific examples.

In the present invention, unless otherwise specified, the raw materials and equipment used can be purchased from the market or commonly used in the field. The methods in the following examples, unless otherwise specified, are conventional methods in the art.

The pH of the phosphate buffer solution used in the present invention is 6.86, and is configured with reference to the method of Chinese Pharmacopoeia (2010 Edition) Part II.

Example 1:

A compound aloe vera polysaccharide external gel for treating burns is prepared by mixing the following raw material components in parts by weight: 20 parts of aloe vera polysaccharide, 50 parts of sodium carboxymethyl cellulose, and 10 parts of glycerin , 1 part of triethanolamine, and phosphate buffer to make up to 1000 parts.

Example 2:

A compound aloe vera polysaccharide external gel for treating burns is prepared by mixing the following raw material components in parts by weight: 80 parts of aloe vera polysaccharide, 5 parts of carbomer-940, sorbitol, polysaccharide 90 parts of ethylene glycol, 5 parts of triethanolamine, and phosphate buffer to make up to 1000 parts.

Example 3:

A compound aloe vera polysaccharide external gel for treating burns is prepared by mixing the following raw material components in parts by weight: 20 parts of aloe vera polysaccharide, 10 parts of nano silver powder (commercially available), methyl fiber 20 parts of vegetarian, 20 parts of glycerol, 2 parts of triethanolamine, and phosphate buffer to make up to 1000 parts.

Example 4:

A compound aloe vera polysaccharide external gel for treating burns is prepared by mixing the following raw material components in parts by weight: 20 parts of aloe vera extract (aloe vera polysaccharide and aloe vera alcohol) The mixture of extracts according to the weight ratio of 20:1), 10 parts of nano zinc powder (commercially available), 30 parts of hydroxypropyl methylcellulose-K4M, 25 parts of glycerol, 2 parts of triethanolamine, and phosphate buffer to make up to 1000 copies.

Example 5:

A compound aloe vera polysaccharide external gel for treating burns is prepared by mixing the following raw material components in parts by weight: 20 parts of aloe vera polysaccharide, 10 parts of nano silver powder, 10 parts of nano zinc powder, 5 parts of Bohm-980, 50 parts of glycerol, 2 parts of triethanolamine, and phosphate buffer to make up to 1000 parts.

Example 6:

A compound aloe vera polysaccharide external gel for treating burns is prepared by mixing the following raw material components in parts by weight: 20 parts of aloe vera extract (aloe vera polysaccharide and aloe vera alcohol) The extract is a mixture in a weight ratio of 25:1), 10 parts of nano silver powder, 10 parts of nano zinc powder, 8 parts of carbomer-980, 50 parts of glycerol, 2 parts of triethanolamine, and phosphate buffer to make up to 1000 parts.

Example 7:

A compound aloe vera polysaccharide external gel for treating burns is prepared by mixing the following raw material components in parts by weight: 25 parts of aloe vera extract (aloe vera polysaccharide and aloe vera alcohol) The extract is a mixture in a weight ratio of 30:1), 7 parts of nano silver powder, 8 parts of nano zinc powder, 4 parts of carbomer-980, 40 parts of glycerol, 4 parts of triethanolamine, and phosphate buffer to make up to 1000 parts.

Aloe vera polysaccharide in the present invention is prepared by the following method:

(1) Crude polysaccharide extraction: fresh six-year-old Aloe vera, washed, de-thorn, soaked in deionized water to remove the yellow juice exuded from the epidermis, peeled, scraped the inner gel, weighed 1000g of the gel, Squeeze the juice for 2 min to obtain a gel liquid, centrifuge the gel liquid (8000 r/min, 15 min), collect the supernatant, concentrate under reduced pressure (the concentration ratio is 1:2), add absolute ethanol to the final concentration of 80% ethanol, Let stand at 4°C for 12h, collect the precipitate, wash the precipitate with absolute ethanol, vacuum dry at 60°C, and pulverize to obtain crude polysaccharide;

(2) Deproteinization: the crude polysaccharide obtained in step (1) is dissolved in deionized water to obtain a crude polysaccharide extract (concentration 1 mg/mL), the crude polysaccharide extract and the deproteinized solution (chloroform and n-butanol in a ratio of 3:1) Volume ratio mixing) according to the volume ratio of 1:0.2-0.3, mix well, centrifuge, collect the supernatant, repeat the above operation 3 times, the obtained supernatant is concentrated in vacuo to remove the organic solvent, freeze-drying to obtain Curacao Aloe vera polysaccharide meal;

(3) Ultrafiltration: Aloe vera polysaccharide coarse powder is dissolved in deionized water, centrifuged, and the supernatant is diluted with deionized water to obtain a solution of aloe vera polysaccharide with a concentration of 0.5 mg/mL. The solution is intercepted by an ultrafiltration membrane, and a product with a molecular weight greater than 100,000 is obtained by intercepting, and the aloe vera polysaccharide is obtained after vacuum drying.

The alcohol extract of Aloe vera of the present invention is prepared by the following method: freeze-drying Aloe vera, and then pulverize it at a low temperature of 2-5 DEG C in a low-temperature ultrafine pulverizer to obtain a low-temperature aloe vera with a fineness of 100-150 meshes. Powder, mix the aloe vera low-temperature powder and the ethanol solution with a mass concentration of 95% according to the material-liquid ratio of 1g: 10-15ml, and ultrasonically extract it for 30-50min under the ultrasonic power of 450-500w, then heat it to 50-60 ℃, extract 1 -2h, filtered with suction, evaporated and concentrated on a rotary evaporator to a viscous state, and freeze-dried. The preparation method takes Example 5 as an example:

First weigh the formula amount of Aloe vera polysaccharide and dissolve it in phosphate buffer;

Weigh the formula amount of matrix and fully swell;

Weigh the amount of nano-silver, nano-zinc and glycerin in the formula and grind them evenly;

Mix the above three, add phosphate buffer to make up, stir at 15-45°C for 1.5-2.5h, and finally add triethanolamine to adjust the pH to about 7.0.

Pharmacodynamic study of compound curacao polysaccharide gel

1. Experimental method

1.1 Preparation of rat second-degree burn model

The rats were anesthetized by intraperitoneal injection with 10% chloral hydrate at 0.3ml/100g body weight, and the rats were depilated at the same position on the back, and the depilation area was cleaned with normal saline. Using 100 ℃ boiling water and cotton gauze pad (24 layers) method, a rat model of back skin scald was created, which was confirmed to be deep second-degree burns by pathological sections. This experiment intends to burn 4cm × 4cm body surface area.

1.2 Animal grouping and handling

80 SD female rats were adaptively fed for one week after repurchase, grouped after weighing, and divided into blank control group (n=10): normal feeding; model control group (n=10): normal feeding after successful modeling; Positive control group (n=10): After successful modeling, silver sulfadiazine cream was applied to the wound area daily at a dose of 1.0 g/wound/day (containing Ag ⁺ 10 mg).

Aloe vera polysaccharide gel group (n=10, 20 parts of Aloe vera polysaccharide + 9805 parts of carbomer + 50 parts of glycerol + 2 parts of triethanolamine, phosphate buffer to make up to 1000 parts): modeling After success, the wound area was coated with polysaccharide gel every day at a dose of 1.0 g/wound/day (containing 20 mg of aloe vera polysaccharide).

Zinc-silver gel group (n=10, 10 parts of nano-silver powder + 10 parts of nano-zinc powder + 9805 parts of carbomer + 50 parts of glycerol + 2 parts of triethanolamine + phosphate buffer to make up to 1000 parts): successful modeling After that, the wound area was coated with zinc-silver compound gel every day at a dose of 1.0 g/wound/day (containing 10 mg of Ag and Zn each).

Compound gel low-dose group (n=10): After successful modeling, the gel was applied to the wound area every day at a dose of 0.5 g/wound/day (containing 5 mg of Ag and Zn, 5 mg of Aloe vera polysaccharide). 10 mg).

Compound gel medium-dose group (n=10): After successful modeling, the gel was applied to the wound area every day at a dose of 1.0 g/wound/day (containing 10 mg each of Ag and Zn, and Aloe vera polysaccharide). 20mg).

Compound gel high-dose group (n=10): After successful modeling, the gel was applied to the wound area every day at a dose of 2.0 g/wound/day (containing 20 mg of Ag, Zn, and Aloe vera polysaccharide). 40mg).

Compound gel medium dose plus alcohol extract group (n=10): After successful modeling, the gel was applied to the wound area every day at a dose of 1.0 g/wound/day (containing 10 mg each of Ag and Zn, Aloe vera polysaccharide 19.23mg + Aloe vera alcohol extract 0.77mg).

A total of 18d was administered.

2. Experimental results

2.1 Effects of drugs on wound healing in burn rats

2.2 Rat wound epithelization rate (ER)

On the 1st day after burn, the wound ER of the rats in each group was similar, and on the 6th, 12th and 18th days, the ER of the rats in each administration group was significantly higher than that in the model group, with statistical significance (P<0.05, n=10); The ER of the aloe polysaccharide gel group, silver zinc gel group and compound gel low-dose group was lower than that of the positive control group, with statistical significance (P<0.05, n=10). The effect of drugs on the ER of the rats in the high-dose gel group was basically the same as that in the positive control group; the effect of the compound gel medium dose plus alcohol extract group on the ER of the rats was higher than that in the positive control group; the low-dose compound gel group had a higher effect on the ER of the rats There was almost no difference with the curacao aloe polysaccharide gel group; the ER of the rats in the compound gel medium dose group, the compound gel high dose group, and the compound gel medium dose plus alcohol extract group was significantly higher than that in curacao aloe polysaccharide gel. group, with statistical significance (P<0.05, n=10); the ER of the compound gel low-dose group was lower than that of the silver-zinc gel group; the ER of the compound gel medium-dose group was slightly higher than that of the silver-zinc gel group ; The ER of the rats in the high-dose compound gel group was significantly higher than that in the silver-zinc gel group, and the ER of the rats in the compound gel medium-dose plus alcohol extract group was higher than that in the silver-zinc gel group, with statistical significance (P<0.05, n=10) The results are shown in Table 1.

Table 1 The effect of compound aloe vera polysaccharide gel on rat ER ( n=10)

Note: *P<0.05, the comparison between the administration group and the model group, ▲P<0.05, the comparison between the administration group and the positive control group; △P<0.05, the comparison between the compound gel group and the polysaccharide gel group; ●P<0.05, Comparison between compound gel group and silver-zinc gel group

2.3 Detection of hydroxyproline (Hyp) protein level in rat wound tissue

From the 6th day to the 18th day after the burn, the Hyp content in the wounds of the burned rats continued to increase, and the Hyp content in each administration group was significantly higher than that in the model group, with statistical significance (P<0.05, n=10). The Hyp content of the wound promotes skin growth and healing; on the 6th and 12th days, the Hyp content of the polysaccharide group was significantly lower than that of the positive control group, with statistical significance (P<0.05, n=10), and on the 18th day, it was significantly higher than that of the positive control group, There was statistical significance (P<0.05, n=10); the content of Hyp in the silver-zinc gel group was significantly lower than that in the positive control group (P<0.01, n=10); the content of Hyp in the low-dose compound gel group lower than that of the positive control group, with statistical significance (P<0.01, n=10); the content of Hyp in the middle-dose compound gel group, the compound gel high-dose group, and the compound gel medium-dose plus alcohol extract group increased at 18d after burn In the positive control group, it was statistically significant (P<0.01, n=10); the experimental results indicated that the compound aloe vera gel could effectively increase the Hyp content of the wound and promote wound healing in the later stage of burn treatment. The results are shown in Table 2.

Table 2 The effect of compound curacao polysaccharide gel on the content of Hyp in rat wounds ( n=10)

Note: ☆P<0.05, ☆☆P<0.01, the model group was compared with the blank group; *P<0.05, **P<0.01, the treatment group was compared with the model group; ▲P<0.05, ▲▲P<0.01, Compared with the positive control group; △P<0.05, △△P<0.01, the compound gel group was compared with the polysaccharide gel group; ●P<0.05, ●●P<0.01, the compound gel group was compared with the silver zinc gel group Glue group comparison

2.4 Detection of epidermal growth factor (EGF) protein levels in rat wound tissue

From the 6th day to the 18th day after burn, the content of EGF in the wound of the rats in the positive control group, the high-dose compound gel group, and the compound gel medium-dose plus alcohol extract group was significantly higher than that in the model group, with statistical significance (P<0.01, n=10). ), there was no significant difference between the compound gel high-dose group and the positive control group, and the compound gel medium-dose plus alcohol extract group was significantly different from the positive control group. Both with silver sulfadiazine cream can rapidly and significantly increase the EGF content of wounds, and the therapeutic effects of the two are equivalent; the therapeutic effect of compound aloe vera polysaccharide plus alcohol extract gel at a medium dose is better than that of silver sulfadiazine cream; other The EGF content of each administration group was significantly higher than that of the model group at 18 days, with statistical significance (P<0.01, n=10). There was no significant difference in the zinc group. The EGF content of the compound gel high-dose group was higher than that of the polysaccharide gel group and the silver-zinc gel group. Gel group. The results are shown in Table 3.

Table 3 Effects of compound curacao polysaccharide gel on EGF content in rat wounds ( n=10)

Note: ☆P<0.05, ☆☆P<0.01, the model group was compared with the blank group; *P<0.05, **P<0.01, the treatment group was compared with the model group; ▲P<0.05, ▲▲P<0.01, Compared with the positive control group; △P<0.05, △△P<0.01, the compound gel group was compared with the polysaccharide gel group; ●P<0.05, ●●P<0.01, the compound gel group was compared with the silver zinc gel group Glue group comparison.

The above-mentioned embodiment is only a preferred solution of the present invention, and does not limit the present invention in any form, and there are other variations and modifications under the premise of not exceeding the technical solution recorded in the claims.

Claims (6)

1. it is a kind of for treat burn compound aloe barbadensis Miller polysaccharide external-use gel, it is characterised in that: by weight by with Lower raw material components are mixed, the raw material composition of 1000 parts by weight are as follows: and 20-80 parts of main ingredient, 3-50 parts of matrix, moisturizer 10-90 Part, 1-5 parts of triethanolamine, phosphate buffer complements to 1000 parts；The main ingredient is aloe vera extract or Curacao The combination of one or both of aloe extract and nano-silver powder, nano zinc powder；The aloe vera extract is library drawing Rope aloe polysaccharide and aloe barbadensis Miller alcohol extract according to 20-30: 1 weight ratio mixture；

The aloe barbadensis Miller polysaccharide is prepared by following methods:

(1) Thick many candies extract: fresh sexennial aloe barbadensis Miller, cleaning, deburring, are impregnated in deionized water to remove epidermis exudation Yellow sap, peeling, scrape internal layer gel, weigh gel 1000g, coagulant liquid of squeezing the juice to obtain, coagulant liquid centrifugation, collect supernatant After reduced pressure, dehydrated alcohol is added to ethyl alcohol final concentration of 80% in liquid, and 4 DEG C of standing 12h collect precipitating, precipitate anhydrous second Alcohol washing, 60 DEG C of vacuum drying, obtains Thick many candies after crushing；

(2) take off albumen: adding deionized water dissolving to obtain Thick many candies extracting solution Thick many candies obtained by step (1), Thick many candies extracting solution with De- protein liquid is mixed according to 1: 0.2-0.3 volume ratio, and supernatant is collected in centrifugation, is repeated aforesaid operations 3 times, gained supernatant Vacuum-concentrcted removes organic solvent, and aloe barbadensis Miller polysaccharide coarse powder is obtained after freeze-drying；

(3) ultrafiltration: aloe barbadensis Miller polysaccharide coarse powder adds deionized water dissolving, and centrifugation takes supernatant that deionized water is added to dilute dense Degree is the aloe barbadensis Miller polysaccharide solution of 0.5mg/mL, and aloe barbadensis Miller polysaccharide solution is retained with ultrafiltration membrane, and retention obtains molecule Amount is greater than 100,000 product, and aloe barbadensis Miller polysaccharide is obtained after vacuum drying；

The aloe barbadensis Miller alcohol extract is prepared by following methods: aloe barbadensis Miller is freeze-dried, it is then super in low temperature Low-temperature grinding is handled at 2-5 DEG C in atomizer, the aloe low temperature powder of fineness 100-150 mesh is obtained, by aloe low temperature powder and quality The ethanol solution of concentration 95% is uniformly mixed according to 1g: 10-15ml solid-liquid ratio, and ultrasonic power is ultrasonic extraction under 450-500w 30-50min is then heated to 50-60 DEG C, extracts 1-2h, filters, and is concentrated by evaporation on a rotary evaporator to sticky shape, freezing It is dry.

2. compound aloe barbadensis Miller polysaccharide external-use gel according to claim 1, it is characterised in that: the matrix is selected from card Bo Mu -940, carbomer -934, carbomer -980, hydroxypropyl methyl cellulose-K4M, hydroxypropyl methyl cellulose-K15M, hydroxyl Propyl methocel-K100M, polyethylene glycol-4000, sodium carboxymethylcellulose, methylcellulose, tragacanth, gelatin, gather One or more of vinylpyrrolidone, Polycarbophil, alginate, Sodium Polyacrylate, starch, chitosan.

3. compound aloe barbadensis Miller polysaccharide external-use gel according to claim 2, it is characterised in that: by weight by with Lower raw material components are mixed, the raw material composition of 1000 parts by weight are as follows: and 20 parts of aloe vera extract, 10 parts of nano-silver powder, 10 parts of nano zinc powder, -9805 parts of carbomer, 50 parts of moisturizer, 2 parts of triethanolamine, phosphate buffer complements to 1000 parts.

4. compound aloe barbadensis Miller polysaccharide external-use gel according to claim 1, it is characterised in that: the de- protein liquid is Chloroform and n-butanol are obtained by mixing according to 3: 1 volume ratio.

5. compound aloe barbadensis Miller polysaccharide external-use gel according to claim 1 or 2 or 3, it is characterised in that: the moisturizing Agent is selected from one or more of glycerol, propylene glycol, sorbierite, polyethylene glycol.

6. compound aloe barbadensis Miller polysaccharide external-use gel according to claim 1 or 2 or 3, it is characterised in that: the phosphoric acid The pH of salt buffer is 6.86.