CN105380601A - Device for extracting stromal vascular fractions from autologous fat tissue - Google Patents

Abstract

The invention discloses a device for extracting stromal vascular components from autologous adipose tissue, which is used for extracting and purifying stromal vascular components. The device comprises a fat container tube, the upper end of which is open and the lower end is narrow The fat output port of the device, the device also includes an upper filter bracket sealed at the opening of the upper end of the fat container tube, and a lower filter bracket positioned at the bottom of the fat container tube, the upper filter bracket is provided with a fat injection port and a cleaning solution injection port. An upper filter membrane is arranged between the inlet, the fat injection port and the bottom surface of the upper filter holder, and a lower filter membrane is arranged between the lower filter holder and the fat outlet. The device has the advantages of exquisite structure, simple operation, good applicability, safety and reliability, and provides a new device for extracting and purifying stromal blood vessel components from autologous adipose tissue in the field of biomedical engineering.

Description

A device for extracting stromal vascular components from autologous adipose tissue

technical field

The patent of the invention belongs to the field of biomedical engineering and relates to a device for extracting stromal blood vessel components from autologous adipose tissue.

Background technique

Individualized treatment is a symbolic progress of modern medicine. Cell therapy including stem cells embodies the core of translational medicine and individualized treatment. Stromal Vascular Fraction (SVF) refers to the adipose tissue obtained after removing mature adipocytes. Stromal cells with stem cell properties are a group of mixed cell populations. The stromal vascular components include: adipose precondition cells, mesenchymal stem cells, hematopoietic stem (progenitor) cells, endothelial progenitor cells, lymphocytes (including regulatory T cells), and macrophages. Clinically, it has obvious curative effect on diabetes, systemic lupus erythematosus and lupus nephritis. In the beauty industry, the stromal blood vessel components are reinfused into the patient's body for cosmetic operations such as wrinkle removal and breast augmentation, which have the advantages of less immune rejection, long-lasting retention time, and good cosmetic effects.

However, there is currently a lack of simple and efficient methods for obtaining stromal vascular fractions from adipose tissue.

Contents of the invention

The purpose of the present invention is to provide a device for extracting stromal blood vessel components from autologous adipose tissue, and obtain the stromal blood vessel components by impacting with hand-operated marbles, thereby providing a set of simple and feasible devices for clinical treatment.

In order to achieve the above object, the technical scheme of the present invention is as follows:

A device for extracting stromal vascular components from autologous adipose tissue to obtain stromal vascular components, the device includes a fat container tube, the upper end of the fat container tube is an opening, and the lower end is a narrowed fat output port, the device It also includes an upper filter bracket sealed at the opening of the upper end of the fat container tube, and a lower filter bracket positioned at the bottom of the fat container tube, the upper filter bracket is provided with a fat injection port and a cleaning solution injection port, and the fat injection port is connected to the fat container tube. An upper filter membrane is arranged between the bottom surfaces of the upper filter holder, and a lower filter membrane is arranged between the lower filter holder and the fat outlet.

Further, a waste storage cavity is provided between the upper filter membrane and the fat injection port for storing filtered impurities.

Further, steel balls are placed inside the fat container tube, the bottom surface of the upper filter bracket is set as a mesh upper annular hole corresponding to the position of the upper filter membrane, and the position of the lower filter bracket corresponding to the lower filter membrane is set as a mesh Lower annular hole. There are 3-7 stainless steel marbles placed inside the fat container tube, the purpose is to hit each other during the shaking process, crush the fat particles and release the component cells, which is more conducive to the homogenization of fat.

Further, an upper sealing ring is arranged between the upper filter bracket and the inner wall of the fat container tube, and a lower sealing ring is arranged between the lower filter bracket and the inner wall of the fat container tube.

The edge of the upper opening of the fat container tube of the present invention extends outwards, the upper part of the upper filter bracket correspondingly extends outwards to adapt to the edge of the upper opening of the fat container tube, and the lower part of the upper filter bracket adapts to the diameter of the fat container tube. During installation, the lower part of the upper filter bracket extends into the inside of the fat container tube, and the upper part of the upper filter bracket is sealed on the edge of the fat container tube.

Further, the upper filter bracket is also provided with an air outlet, so that free air can be exchanged between the inside and outside of the device, keeping the fat in a normal pH environment, and at the same time allowing the extraction of waste liquid and stromal blood vessel components.

Further, the fat injection port, the cleaning liquid injection port and the air outlet are also provided with one-way valves. Prevent the reverse flow of liquid and air.

The invention proposes a device for extracting stromal blood vessel components from autologous adipose tissue, which cleans and purifies the autologous fat in the fat container tube through the injection and discharge of cleaning liquid, and quickly shakes the fat container up and down manually Use a tube to filter out useless connective tissue, put a number of steel balls in the fat container tube, and use the collision of the steel balls to achieve the effect of crushing and homogenizing the fat in the container tube. The device of the present invention facilitates the extraction, purification and homogenization of cells in various components of human adipose tissue in the field of biomedical engineering, and provides a new method for obtaining stromal blood vessel component cells with high purification degree and rich in various growth factors. s method. It is of great significance to obtain high-quality autologous reinfused stromal vascular components, improve the therapeutic effect, shorten the time of cosmetic surgery, improve the quality of cosmetic surgery, and achieve good postoperative results.

Description of drawings

Fig. 1 is the outline drawing of the device for extracting stromal blood vessel components of the present invention;

Fig. 2 is a cross-sectional view of the device for extracting stromal blood vessel components according to the present invention.

The reference signs in the figure are explained as follows:

1. Fat container tube; 2. Upper filter bracket; 3. Fat injection port; 4. Cleaning liquid injection port; 5. Air outlet; 6. Fat output port; 7. Lower sealing ring; 8. Lower filter bracket; 9. Upper annular hole; 10, waste storage cavity; 12, upper sealing ring; 13, upper filter membrane; 14, lower annular hole; 15, lower filter membrane.

detailed description

The technical solution of the present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments, and the following embodiments do not constitute a limitation of the present invention.

As shown in Figures 1 and 2, the present embodiment is a device for extracting stromal vascular components from autologous adipose tissue. It is an opening, and the lower end is a narrowed fat output port 6. The upper filter bracket 2 is sealed at the upper opening of the fat container tube 1, and the lower filter bracket 8 is located at the bottom of the fat container tube 1.

The upper filter bracket 2 is provided with a fat injection port 3 and a cleaning solution injection port 4, an upper filter membrane 13 is arranged between the fat injection port 3 and the bottom surface of the upper filter bracket 2, and an upper filter membrane 13 is arranged between the upper filter membrane 13 and the fat injection port 3. The waste storage cavity 10 ; the lower filter membrane 15 is arranged between the lower filter holder 8 and the fat output port 6 .

The autologous fat taken out of the human body is injected into the fat container tube 1 through the upper filter membrane 13 through the fat injection port 3 for processing, and the impurities in the fat will be filtered out by the upper filter membrane 13 and left in the upper part of the upper filter membrane 13 for waste storage cavity 10. Then, the cleaning solution meeting the GMP standard is injected into the fat container tube 1 through the cleaning solution injection port 4 to clean and purify the fat. After the fat is processed, the waste liquid is extracted from the fat outlet 6, and the upper part of the fat outlet 6 is provided with a lower filter membrane 15 to prevent the purified fat from losing. Finally, a syringe is used to connect the lower filter membrane 15 to draw out the extracted stromal blood vessel components from the fat output port 6 .

Steel balls are placed inside the fat container tube 1 of this embodiment, the bottom surface of the upper filter bracket 2 corresponding to the position of the upper filter membrane 13 is set as a mesh upper annular hole 9, and the position of the lower filter bracket 8 corresponding to the lower filter membrane 15 is set as a mesh Lower annular hole 14.

Put several steel balls in the fat container tube 1 in advance, after injecting fat and cleaning solution into the fat container tube 1, manually shake the fat container tube 1 up and down quickly for a period of time, the steel balls in the fat container tube 1 will also be cleaned together with the fat The liquid mixture vibrates up and down together, and the collision of steel balls achieves the effect of crushing and homogenizing the fat in the fat container tube 1. Setting the upper annular hole 9 and the lower annular hole 14 not only prevents the steel ball from rushing out of the fat container tube 1, but also facilitates the homogenization of the fat by hitting the annular hole, thereby cleaning, purifying, crushing and homogenizing the fat. If it is not set as a mesh annular hole, the steel ball will easily cause damage to the upper and lower filter membranes, and the fat cannot be effectively crushed and homogenized.

In this embodiment, an upper sealing ring 12 is arranged between the upper filter bracket 2 and the inner wall of the fat container tube 1, and a lower sealing ring 7 is arranged between the lower filter bracket 8 and the inner wall of the fat container tube 1 to increase the airtightness and prevent the shaking process spillage of liquid.

In this embodiment, the edge of the upper opening of the fat container tube 1 extends outwards, and the top of the upper filter bracket 2 extends outwards correspondingly to the edge of the upper end opening of the fat container tube 1. When installing, the bottom of the upper filter bracket 2 extends into the inside of the fat container tube 1, and the top of the upper filter bracket 2 is sealed on the edge of the fat container tube 1.

In this embodiment, the filter bracket 2 is also provided with an air outlet 5. The air outlet 5 enables free air exchange between the inside and outside of the device, keeps the fat in a normal pH environment, and simultaneously facilitates the extraction of waste liquid and fat. It is also possible not to set the air outlet 5, and directly use the cleaning liquid injection port 4 as the air outlet.

It should be noted that the fat injection port 3, the cleaning liquid injection port 4 and the air outlet port 5 are also provided with one-way valves, which can only be injected but not flowed out, so as to prevent the liquid from flowing out when shaking.

The fat container tube 1, the upper filter support 2 and the lower filter support 8 of the present invention are made of polymer materials, which are convenient for one-time use and recycling, and are economical and environmentally friendly.

In this embodiment, the operation steps of the device for extracting stromal vascular components from autologous adipose tissue are as follows:

1. Unscrew the upper filter bracket 2 on the upper end of the fat container tube 1 as a whole, put several steel balls into the fat container tube 1, and then screw the upper filter bracket 2 into the upper end of the fat container tube 1 as a whole;

2. Use a syringe to inject human fat tissue through the fat injection port 3, and then inject an appropriate amount of cleaning solution from the cleaning solution injection port 4;

3. Manually shake the fat container tube 1 up and down quickly for a period of time, extract the waste liquid from the fat output port 6, re-inject an appropriate amount of clean and sterile cleaning solution, and perform the second manual shaking operation.

4. Repeat step 3. After the fat extraction and purification of the stromal vascular components are completed, a syringe is used to connect the fat output port 6, and the treated stromal vascular components are extracted for clinical application.

The device of the present invention completes the manual extraction and purification of stromal vascular components from autologous adipose tissue, can obtain more pure stromal vascular components, and can uniformly mix various components in adipose tissue. It has the advantage of delicate structure and easy operation The device is simple, has good applicability, is safe and reliable, and provides a new device for extracting and purifying stromal blood vessel components from autologous adipose tissue in the field of biomedical engineering.

The above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Without departing from the spirit and essence of the present invention, those skilled in the art can make various corresponding changes and changes according to the present invention. deformation, but these corresponding changes and deformations should belong to the scope of protection of the appended claims of the present invention.

Claims (8)

1. A device for extracting stromal blood vessel components from autologous adipose tissue, characterized in that the device comprises a fat container tube (1), the upper end of the fat container tube (1) is an opening, and the lower end is narrowed fat output port (6), the device also includes an upper filter support (2) capped at the opening of the upper end of the fat container tube (1), and a lower filter support (8) positioned at the bottom of the fat container tube (1), the The upper filter bracket (2) is provided with a fat injection port (3) and a cleaning solution injection port (4), and an upper filter membrane (13) is arranged between the fat injection port (3) and the bottom surface of the upper filter bracket (2) , a lower filter membrane (15) is arranged between the lower filter bracket (8) and the fat outlet (6). 2. The device for extracting stromal vascular components according to claim 1, characterized in that a waste storage cavity (10) is provided between the upper filter membrane (13) and the fat injection port (3). 3. The device for extracting stromal vascular components according to claim 1, characterized in that steel balls are placed inside the fat container tube (1). 4. The device for extracting stromal blood vessel components according to claim 3, characterized in that, the position corresponding to the upper filter membrane (13) on the bottom surface of the upper filter holder (2) is set as a mesh upper annular hole (9) , the position of the lower filter support (8) corresponding to the lower filter membrane (15) is set as a mesh lower annular hole (14). 5. The device for extracting stromal blood vessel components according to claim 1, characterized in that, an upper sealing ring (12) is arranged between the upper filter support (2) and the inner wall of the fat container tube (1), the A lower sealing ring (7) is arranged between the lower filter support (8) and the inner wall of the fat container tube (1). 6. The device for extracting stromal blood vessel components according to claim 1, characterized in that, the edge of the upper opening of the fat container tube (1) extends outwards, and the top of the upper filter bracket (2) extends outwards correspondingly Adapt to the edge of the upper opening of the fat container tube (1), the lower part of the upper filter bracket (2) is adapted to the diameter of the fat container tube (1), and when installed, the lower part of the upper filter bracket (2) extends into the fat container Inside the tube (1), the upper part of the upper filter holder (2) is capped on the edge of the fat container tube (1). 7. The device for extracting stromal vascular components according to claim 1, characterized in that an air outlet (5) is also provided on the upper filter holder (2). 8. The device for extracting stromal vascular components according to claim 7, characterized in that, the fat injection port (3), cleaning solution injection port (4) and air outlet (5) are also provided with one-way valves.