CN105368899A - Method for extracting water-soluble beta glucan from pleurotus ferulae lenzi sporocarp - Google Patents
Method for extracting water-soluble beta glucan from pleurotus ferulae lenzi sporocarp Download PDFInfo
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- 229920002498 Beta-glucan Polymers 0.000 title claims abstract description 31
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 24
- 235000006521 Pleurotus eryngii var ferulae Nutrition 0.000 title abstract description 12
- 244000088486 Pleurotus eryngii var. ferulae Species 0.000 title abstract description 12
- 150000004676 glycans Chemical class 0.000 claims abstract description 53
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 53
- 239000005017 polysaccharide Substances 0.000 claims abstract description 53
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 27
- 239000003513 alkali Substances 0.000 claims abstract description 23
- 230000007062 hydrolysis Effects 0.000 claims abstract description 21
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 21
- 238000000605 extraction Methods 0.000 claims abstract description 16
- 238000003756 stirring Methods 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000000843 powder Substances 0.000 claims abstract description 15
- 238000001035 drying Methods 0.000 claims abstract description 14
- 238000000926 separation method Methods 0.000 claims abstract description 12
- 102000004139 alpha-Amylases Human genes 0.000 claims abstract description 11
- 108090000637 alpha-Amylases Proteins 0.000 claims abstract description 11
- 229940024171 alpha-amylase Drugs 0.000 claims abstract description 11
- 238000010438 heat treatment Methods 0.000 claims abstract description 7
- 238000001914 filtration Methods 0.000 claims abstract description 5
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 76
- 239000000706 filtrate Substances 0.000 claims description 25
- 238000001556 precipitation Methods 0.000 claims description 21
- 229920002307 Dextran Polymers 0.000 claims description 17
- 239000004744 fabric Substances 0.000 claims description 15
- 239000002253 acid Substances 0.000 claims description 13
- 229960000935 dehydrated alcohol Drugs 0.000 claims description 7
- 229960004756 ethanol Drugs 0.000 claims description 6
- 239000000284 extract Substances 0.000 claims description 3
- 239000007788 liquid Substances 0.000 abstract description 7
- 238000002360 preparation method Methods 0.000 abstract description 5
- 238000012356 Product development Methods 0.000 abstract description 3
- 238000010521 absorption reaction Methods 0.000 abstract description 3
- 235000019441 ethanol Nutrition 0.000 abstract 2
- 239000013049 sediment Substances 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 1
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 62
- 239000011148 porous material Substances 0.000 description 12
- 239000000047 product Substances 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- 229940088598 enzyme Drugs 0.000 description 5
- 238000002329 infrared spectrum Methods 0.000 description 4
- CTYRPMDGLDAWRQ-UHFFFAOYSA-N phenyl hydrogen sulfate Chemical compound OS(=O)(=O)OC1=CC=CC=C1 CTYRPMDGLDAWRQ-UHFFFAOYSA-N 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 241000233866 Fungi Species 0.000 description 3
- 150000001298 alcohols Chemical class 0.000 description 3
- 230000002538 fungal effect Effects 0.000 description 3
- 238000005227 gel permeation chromatography Methods 0.000 description 3
- 239000007791 liquid phase Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000010298 pulverizing process Methods 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 238000005903 acid hydrolysis reaction Methods 0.000 description 2
- 230000001093 anti-cancer Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 150000007530 organic bases Chemical class 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 2
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 2
- UGZADUVQMDAIAO-UHFFFAOYSA-L zinc hydroxide Chemical compound [OH-].[OH-].[Zn+2] UGZADUVQMDAIAO-UHFFFAOYSA-L 0.000 description 2
- 229910021511 zinc hydroxide Inorganic materials 0.000 description 2
- 229940007718 zinc hydroxide Drugs 0.000 description 2
- 241000222485 Agaricales Species 0.000 description 1
- 241000222382 Agaricomycotina Species 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 241000222336 Ganoderma Species 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- XUYPXLNMDZIRQH-LURJTMIESA-N N-acetyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC(C)=O XUYPXLNMDZIRQH-LURJTMIESA-N 0.000 description 1
- 208000001132 Osteoporosis Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241001492261 Pleurotaceae Species 0.000 description 1
- 241000222350 Pleurotus Species 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 208000037386 Typhoid Diseases 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000002605 anti-dotal effect Effects 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000011651 chromium Substances 0.000 description 1
- 229910052804 chromium Inorganic materials 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 229910052732 germanium Inorganic materials 0.000 description 1
- GNPVGFCGXDBREM-UHFFFAOYSA-N germanium atom Chemical compound [Ge] GNPVGFCGXDBREM-UHFFFAOYSA-N 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 208000007442 rickets Diseases 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 201000008297 typhoid fever Diseases 0.000 description 1
- 238000004260 weight control Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
- C12P19/08—Dextran
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- Engineering & Computer Science (AREA)
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- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
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- Polysaccharides And Polysaccharide Derivatives (AREA)
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Abstract
The invention provides a method for extracting water-soluble beta glucan from pleurotus ferulae lenzi sporocarp. The method includes the following steps: 1, preparation of pleurotus ferulae lenzi coarse polysaccharide, wherein pleurotus ferulae lenzi sporocarp is picked and crushed, powder is obtained, the powder is extracted with alkali liquor, filtration is conducted to remove residues, ethyl alcohol is added to the filter liquor, the filter liquor stands still and is filtered, sediment is obtained, and the pleurotus ferulae lenzi coarse polysaccharide is obtained; 2, preparation of pleurotus ferulae lenzi beta sporocarp, wherein the pleurotus ferulae lenzi coarse polysaccharide is picked and dried, then water is added to the dried pleurotus ferulae lenzi coarse polysaccharide, stirring is conducted, alpha amylase is added to the mixture for hydrolysis, the hydrolysis liquid is filtered, filter liquor is removed, residues are obtained, and the pleurotus ferulae lenzi beta sporocarp is obtained; 3, preparation of the water-soluble beta glucan, wherein the pleurotus ferulae lenzi beta sporocarp is picked, alkali liquor is added, stirring and extraction are conducted, the pH value of the extraction liquor is adjusted to 3.5-6.8, heating and hydrolysis are conducted, the hydrolysis liquid is filtered, ethyl alcohol is added to the filter liquor, the liquor stands still, separation, sediment and drying are conducted, and the water-soluble beta glucan is obtained. By means of the method, the problem of water solubility of the beta glucan is solved, and absorption, utilization and product development of the pleurotus ferulae lenzi beta sporocarp are more facilitated.
Description
Technical field
the activeconstituents that the present invention relates to Chinese medicine extracts field, particularly a kind of method extracting water-soluble beta dextran from Resina Ferulae mushroom sporophore.
Background technology
resina Ferulae mushroom has another name called Pleurotus ferulae Lanzi, belong to Basidiomycotina, Hymenomycetes, Agaricales, Pleurotaceae, pleurotus is the edible mushrooms of the medicinal and edible dual-purpose that a kind of aridity grass land grows, be described as good names such as " the white glossy ganoderma in Western Paradise " " boletes on grassland ", be mainly distributed in the ground such as India, France, Xinjiang, China.Nineteen eighty-three China scientific worker carries out artificial training cultivation to wild edible Resina Ferulae mushroom and obtains successfully.At present, started in Barkol county to promote, and obtained good economic and social benefit.
the sporophore of Resina Ferulae mushroom is as white as polished jade, fine and tender taste, plump crisp cunning, people attacked by giving off a strong fragrance, unique flavor, detect through state food quality surveillance inspection center and show: Resina Ferulae mushroom contains the trace elements such as very high fungus polysaccharide, selenium, germanium, chromium, Vc, Ve, Vd, Va unsaturated fatty acids, and 17 seed amino acids of needed by human.Modern pharmacology experiment proves, fungus polysaccharide has enhancing body immunity, physiological equilibrium, anticancer, the effect of anti-cancer, unsaturated fatty acids, SOD has prevention of arterial to harden, lowering blood pressure, antidotal effect, Methionin, arginine has protection liver, strengthen effect of children ' s intelligence development, Vd, Va can promote human calcium, phosphorus absorbs, prevent children rachitis, the choice drug of richets and middle-aged and old osteoporosis, stomach trouble is being treated when the Resina Ferulae mushroom that utilizes among the people on ground, typhoid fever, the various diseases such as reducing blood-fat what, especially the blackbone Chicken cooking is edible, to pregnant woman, postpartum what blood, the large benefit effects such as tonifying Qi is empty.
along with the application of Resina Ferulae mushroom is more and more extensive, the activeconstituents of Resina Ferulae mushroom is extracted in order to a more popular problem.Resina Ferulae mushroom polysaccharide is one of main active ingredient of Resina Ferulae mushroom, and the extracting solution for Resina Ferulae mushroom polysaccharide has had some to report.But these documents are all the extraction to Resina Ferulae mushroom total polysaccharides, Resina Ferulae mushroom polysaccharide coexists with α and β two kinds of configurations in Resina Ferulae mushroom, the separation of these two kinds of configurations all do not mentioned by the current document for the technical study of Resina Ferulae mushroom total polysaccharides, only start with from extraction total polysaccharides itself, the total polysaccharides extracted is further purified, is separated the polysaccharide all one obtaining a certain configuration.
polysaccharide molecular weight from several thousand to millions of not etc., molecular weight is larger, and it is water-soluble poorer, and Resina Ferulae mushroom polysaccharide is no exception.Resina Ferulae mushroom polysaccharide macro-molecular amount part aqueous is poor, makees solvent be difficult to extract completely in separation and Extraction with water, even if use alkali lye to extract, although extraction yield is high, is still not dissolved in water when obtaining polysaccharide product application after drying.Human body absorbs bad for this macromole Resina Ferulae mushroom polysaccharide.
Summary of the invention
the object of the present invention is to provide a kind of method extracting water-soluble beta dextran from Resina Ferulae mushroom sporophore, solve the water solubility problems of beta glucan, more be conducive to absorbing and product development of Resina Ferulae mushroom beta glucan, improve the yield of Resina Ferulae mushroom polysaccharide, add water-soluble, be more conducive to absorption of human body.
object of the present invention is achieved through the following technical solutions:
from Resina Ferulae mushroom sporophore, extract a method for water-soluble beta dextran, comprise the following steps:
step 1, prepare Resina Ferulae mushroom Crude polysaccharides:
get Resina Ferulae mushroom sporophore, pulverize and obtain powder, powder alkali lye lixiviate, cross and filter residue, add ethanol in filtrate, leave standstill, filtration is precipitated, and obtains Resina Ferulae mushroom Crude polysaccharides;
step 2, prepare Resina Ferulae mushroom beta glucan:
get Resina Ferulae mushroom Crude polysaccharides, add water after drying stirring, and add α-amylase hydrolysis, hydrolyzed solution filters, and discards filtrate, obtains residue, obtain Resina Ferulae mushroom beta glucan;
step 3, prepare water-soluble beta dextran:
get Resina Ferulae mushroom beta glucan, add alkali lye and stir extraction, extracting solution acid adjustment basicity is to pH3.5-6.8, and heating hydrolysis, hydrolyzed solution filters, and adds ethanol in filtrate, leaves standstill, and precipitation separation is also dry, obtains water-soluble beta dextran.
in described step 1, cross 40 object sieves after Resina Ferulae mushroom sporophore being pulverized, obtain powder.
in described step 1,1-10 hour is got in the alkali lye lixiviate adding 5-20 times of weight in powder, and the concentration of alkali lye used is preferably 0.1-1.0 mol/L.
in described step 1, filter and adopt filter cloth to filter, wherein filter cloth aperture is 0.5-5 micron.
in described step 1 or step 3, the dehydrated alcohol adding 1-4 times of volume in filtrate leaves standstill 2 hours.
in described step 2, the water adding 5-10 times of weight after the drying of Resina Ferulae mushroom Crude polysaccharides stirs, and fully dissolves.
in described step 3, concentration of lye is 0.1-0.4 mol/L; Described heating hydrolysis is preferably heated to 80-100 DEG C, and hydrolysis time is 1-4 hour.
beneficial effect of the present invention:
1, the suitability for industrialized production problem extracting beta glucan in Resina Ferulae mushroom sporophore is solved.What prior art extracted extraction usually is total polysaccharides, and method of the present invention extraction obtains beta glucan, and can be applicable to industrial production.
2, solve the water solubility problems of beta glucan, be more conducive to absorbing and product development of Resina Ferulae mushroom beta glucan.The water insoluble the present invention of the dextran that molecular weight is large utilizes acid hydrolysis technology to reduce the molecular weight of macromolecule Resina Ferulae mushroom polysaccharide.Improve the yield of Resina Ferulae mushroom polysaccharide, add water-soluble, be more conducive to absorption of human body.
Embodiment
embodiment 1
take Resina Ferulae mushroom sporophore 1 kilogram pulverizing, cross 50 objects sieves, powder adds sodium hydroxide solution 5 liters of lixiviates 1 hour of 0.1 mol/L, and the plate basket of 0.5 micron pore size filter cloth is crossed and filtered residue, obtains filtrate 4.7 liters.Filtrate adds the dehydrated alcohol of 18.8 liters, leaves standstill after 2 hours and filters to obtain precipitation with the plate basket of 0.5 micron pore size cloth, weigh totally 150 grams after precipitation drying.Precipitation adds 750 ml water stirring at room temperature 10 minutes, adds 1 gram of Fungal Alpha amylase liquid (Shandong Jie Nuo biological enzyme company limited), and with the salt acid for adjusting pH value to 5.5 of 6 mol/L, 50 degrees Celsius are incubated 24 hours.Then filter with the plate basket of 0.5 micron pore size cloth, discard filtrate, residue adds 0.8 liter, 1.0 mol/L sodium hydroxide and stirs extraction 2 hours, extracting solution acid adjustment basicity is to pH2.0, in 90 degrees Centigrade sealing hydrolysis 1 hour, the plate basket of hydrolyzed solution 0.5 micron pore size cloth filters, filtrate adds 3.2 liters of dehydrated alcohols, leave standstill precipitation separation after 2 hours and drying is weighed as 110 grams of infrared spectras shows that this product is beta glucan, it is 45% that Phenol-sulphate acid method surveys polysaccharide content, and it is 2060 dalton that high performance liquid phase gel chromatography records molecular-weight average; After testing, obtain beta glucan and have good water-soluble.
embodiment 2
take Resina Ferulae mushroom sporophore 1 kilogram pulverizing, cross 50 objects sieves, powder adds sodium hydroxide solution 10 liters of lixiviates 5 hours of 1 mol/L, and the plate basket of 0.5 micron pore size filter cloth is crossed and filtered residue, obtains filtrate 7.2 liters.Filtrate adds the dehydrated alcohol of 28.8 liters, leaves standstill after 2 hours and filters to obtain precipitation with the plate basket of 0.5 micron pore size cloth, weigh totally 142 grams after precipitation drying.Precipitation adds 1.4 premium on currency stirring at room temperature 10 minutes, adds 0.6 gram of Fungal Alpha amylase liquid (purchased from Cheng Xing bio tech ltd, Wuxi), and with the salt acid for adjusting pH value to 5.8 of 2 mol/L, 50 degrees Celsius are incubated 24 hours.Then filter with the plate basket of 0.5 micron pore size cloth, discard filtrate, residue adds 0.8 liter, 1.0 mol/L sodium hydroxide and stirs extraction 2 hours, extracting solution acid adjustment basicity is to pH6.8, in 100 degrees Centigrade sealing hydrolysis 4 hours, the plate basket of hydrolyzed solution 0.5 micron pore size cloth filtered, and filtrate adds 3.2 liters of dehydrated alcohols, leave standstill precipitation separation drying is weighed as 102 grams after 2 hours, be thing of the present invention.Infrared spectra shows that this product is beta glucan, and it is 48% that Phenol-sulphate acid method surveys polysaccharide content, and it is 198056 dalton that high performance liquid phase gel chromatography records molecular-weight average; After testing, obtain beta glucan and have good water-soluble.
embodiment 3
take Resina Ferulae mushroom sporophore 1 kilogram pulverizing, cross 50 objects sieves, powder adds sodium hydroxide solution 20 liters of lixiviates 10 hours of 0.5 mol/L, and the plate basket of 2 micron pore size filter clothes is crossed and filtered residue, obtains filtrate 19.5 liters.Filtrate adds the dehydrated alcohol of 78 liters, leaves standstill after 2 hours and filters to obtain precipitation with the plate basket of 2 micron pore size cloth, weigh totally 143 grams after precipitation drying.Precipitation adds 1 premium on currency stirring at room temperature 10 minutes, adds 1 gram of Fungal Alpha amylase liquid (Shandong Jie Nuo biological enzyme company limited), pH value to 5.5, and 50 degrees Celsius are incubated 24 hours.Then filter with the plate basket of 4 micron pore size cloth, discard filtrate, residue adds 0.8 liter, 0.6 mol/L sodium hydroxide and stirs extraction 2 hours, extracting solution acid adjustment basicity is to pH5.8, in 80 degrees Centigrade sealing hydrolysis 2 hours, the plate basket of hydrolyzed solution 2 micron pore size cloth filtered, and filtrate adds 3.2 liters of dehydrated alcohols, leave standstill precipitation separation drying is weighed as 101 grams after 2 hours, be thing of the present invention.Infrared spectra shows that this product is beta glucan, and it is 40% that Phenol-sulphate acid method surveys polysaccharide content, and it is 40460 dalton that high performance liquid phase gel chromatography records molecular-weight average; After testing, obtain beta glucan and have good water-soluble.
embodiment 4
the specific descriptions of extracting method of the present invention:
a, Resina Ferulae mushroom sporophore are pulverized and are obtained powder, powder alkali lye lixiviate, and filter cleaner, adds ethanol in filtrate, and leave standstill, filtration is precipitated; This precipitation and Resina Ferulae mushroom Crude polysaccharides.Resina Ferulae mushroom beta glucan is a part for Resina Ferulae mushroom Crude polysaccharides, its quite a few be positioned on the cell walls of fungi, in order to extract Resina Ferulae mushroom beta glucan to greatest extent, the present invention selects alkali lye to extract, alkali lye dosage is 5-20 times of raw material weight, the concentration of alkali lye chooses arbitrary concentration in 0.1-1.0 mol/L, extraction time 1-10 hour.Alkali lye can use sodium hydroxide, potassium hydroxide, zinc hydroxide, and the preparations such as organic bases, consider Cost Problems, and alkali lye generally chooses sodium hydroxide solution.Extracting liquid filtering removing residue, filtrate adds the dehydrated alcohol of 1-4 times of volume, leaves standstill and filters to obtain precipitation and Resina Ferulae mushroom raw sugar after 2 hours.
add water after b, precipitation drying stirring, and then add α-amylase hydrolysis, hydrolyzed solution filters, and discards filtrate and obtains residue.
the residue of this step and Resina Ferulae mushroom beta glucan but water-soluble poor.This step adopts the precipitation (Resina Ferulae mushroom Crude polysaccharides) in previous step to be raw material, add α-amylase (because different manufacturers product enzyme activity is different, dosage difference is huge, and those skilled in the art can determine according to the vigor of practical situation and enzyme the consumption adding α-amylase; Consumption is more or a little lessly do not affect effect of the present invention) undissolved for water α configuration polysaccharide hydrolysis is become solvable, discard hydrolyzed solution, simultaneously hydrolyzed solution has also dissolved most of residual monosaccharide in Crude polysaccharides and oligosaccharides, play the effect of purifying, although also a small amount of beta glucan may be there is in hydrolyzed solution, but consider cost recovery, therefore cast out.Only being for further processing to a large amount of beta glucans in residue makes it become solubilized.α-amylase chooses commercially available various models.Hydrolysising condition, enzyme dosage is according to the α-amylase specification sheets of each producer different model.
c, residue add alkali lye and stir extraction, and extracting solution acid adjustment degree is to pH2.0-6.8, and heating hydrolysis, hydrolyzed solution filters, and adds ethanol in filtrate, leaves standstill, and precipitation separation is also dry, to obtain final product.
the residue adopting step b) is raw material, add alkali lye to extract, alkali lye dosage is 5-20 times of raw material weight, extracting solution acid adjustment alkali is to pH2.0-6.8,80-100 DEG C of heating hydrolysis 1-4 hour, hydrolyzed solution filters, and the dehydrated alcohol that filtrate adds 1-4 times of volume precipitates, precipitation separation drying is product of the present invention, namely Resina Ferulae mushroom mushroom water-soluble beta dextran afterwards.
alkali alkali lye can use sodium hydroxide, potassium hydroxide, zinc hydroxide, the preparations such as organic bases, preferred sodium hydroxide, and liquid concentration chooses arbitrary concentration in 0.1-1.0 mol/L.PH can use hydrochloric acid, acetic acid, sulfuric acid, acetic acid etc.Any one value in pH2.0-6.8, just different pH, hydrolysis intensity is different, and after hydrolysis, gained final product weight-average molecular weight is different.
carry out being separated and being further purified obtaining high purity polysaccharide to a certain configuration of Resina Ferulae mushroom mushroom although have in prior art, but its separation method and the present invention have essential distinction, at method many uses column chromatography existing after the water extract-alcohol precipitation of routine, Crude polysaccharides is separated, clip a certain specific part in Crude polysaccharides and obtain highly purified Resina Ferulae mushroom polysaccharide for scientific research, this and industrial method of the present invention have remarkable difference, and the present invention is except carrying out except separation and Extraction to the beta comfiguration of Resina Ferulae mushroom polysaccharide, also water-soluble by improve it to the molecular weight control of Resina Ferulae mushroom polysaccharide.The present invention is by acid hydrolysis, and the molecular weight that the method that water redissolves controls Resina Ferulae mushroom polysaccharide enhances the water-soluble of Resina Ferulae mushroom polysaccharide within the specific limits, improves the yield of Resina Ferulae mushroom polysaccharide macro-molecular amount section, is conducive to suitability for industrialized production Resina Ferulae mushroom β water-soluble polysaccharide.
extracting method of the present invention to obtain Resina Ferulae mushroom fruitbody polysaccharide be the beta glucan eliminating α configuration polysaccharide, and plant beta glucan and have good water-soluble.It is beta glucan that infrared spectra shows product of the present invention, and Phenol-sulphate acid method detects polysaccharide content and is greater than 40%, and polysaccharide molecular-weight average 2000-200000 dalton is not etc.
the foregoing is only preferred embodiment of the present invention, is only illustrative for the present invention, and nonrestrictive.Those skilled in the art understand, and can carry out many changes in the scope of the spirit limited in the claims in the present invention to it, amendment, even equivalence, but all will fall within the scope of protection of the present invention.
Claims (7)
1. from Resina Ferulae mushroom sporophore, extract a method for water-soluble beta dextran, it is characterized in that: comprise the following steps:
Step 1, prepare Resina Ferulae mushroom Crude polysaccharides:
Get Resina Ferulae mushroom sporophore, pulverize and obtain powder, powder alkali lye lixiviate, cross and filter residue, add ethanol in filtrate, leave standstill, filtration is precipitated, and obtains Resina Ferulae mushroom Crude polysaccharides;
Step 2, prepare Resina Ferulae mushroom beta glucan:
Get Resina Ferulae mushroom Crude polysaccharides, add water after drying stirring, and add α-amylase hydrolysis, hydrolyzed solution filters, and discards filtrate, obtains residue, obtain Resina Ferulae mushroom beta glucan;
Step 3, prepare water-soluble beta dextran:
Get Resina Ferulae mushroom beta glucan, add alkali lye and stir extraction, extracting solution acid adjustment basicity is to pH3.5-6.8, and heating hydrolysis, hydrolyzed solution filters, and adds ethanol in filtrate, leaves standstill, and precipitation separation is also dry, obtains water-soluble beta dextran.
2. a kind of method extracting water-soluble beta dextran from Resina Ferulae mushroom sporophore according to claim 1, is characterized in that: in described step 1, crosses 40 object sieves, obtain powder after Resina Ferulae mushroom sporophore being pulverized.
3. a kind of method extracting water-soluble beta dextran from Resina Ferulae mushroom sporophore according to claim 1, it is characterized in that: in described step 1,1-10 hour is got in the alkali lye lixiviate adding 5-20 times of weight in powder, and the concentration of alkali lye used is preferably 0.1-1.0 mol/L.
4. a kind of method extracting water-soluble beta dextran from Resina Ferulae mushroom sporophore according to claim 1, is characterized in that: in described step 1, and filter and adopt filter cloth to filter, wherein filter cloth aperture is 0.5-5 micron.
5. a kind of method extracting water-soluble beta dextran from Resina Ferulae mushroom sporophore according to claim 1, is characterized in that: in described step 1 or step 3, and the dehydrated alcohol adding 1-4 times of volume in filtrate leaves standstill 2 hours.
6. a kind of method extracting water-soluble beta dextran from Resina Ferulae mushroom sporophore according to claim 1, is characterized in that: in described step 2, and the water adding 5-10 times of weight after the drying of Resina Ferulae mushroom Crude polysaccharides stirs, and fully dissolves.
7. a kind of method extracting water-soluble beta dextran from Resina Ferulae mushroom sporophore according to claim 1, is characterized in that: in described step 3, and concentration of lye is 0.1-0.4 mol/L; Described heating hydrolysis is preferably heated to 80-100 DEG C, and hydrolysis time is 1-4 hour.
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