CN105238692B - A kind of Multi-function experimental chick embryo culture detection means - Google Patents
A kind of Multi-function experimental chick embryo culture detection means Download PDFInfo
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- CN105238692B CN105238692B CN201510596069.XA CN201510596069A CN105238692B CN 105238692 B CN105238692 B CN 105238692B CN 201510596069 A CN201510596069 A CN 201510596069A CN 105238692 B CN105238692 B CN 105238692B
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- 238000001514 detection method Methods 0.000 title claims abstract description 80
- 210000003837 chick embryo Anatomy 0.000 title claims abstract description 16
- 210000001161 mammalian embryo Anatomy 0.000 claims abstract description 118
- 241000287828 Gallus gallus Species 0.000 claims abstract description 96
- 238000002474 experimental method Methods 0.000 claims abstract description 86
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 29
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 29
- 239000001301 oxygen Substances 0.000 claims abstract description 29
- 230000000694 effects Effects 0.000 claims abstract description 25
- 230000005855 radiation Effects 0.000 claims abstract description 24
- 239000011159 matrix material Substances 0.000 claims abstract description 21
- 238000004140 cleaning Methods 0.000 claims abstract description 17
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000011534 incubation Methods 0.000 claims abstract description 11
- 238000000926 separation method Methods 0.000 claims abstract description 11
- 238000005202 decontamination Methods 0.000 claims abstract description 10
- 230000003588 decontaminative effect Effects 0.000 claims abstract description 10
- 238000007789 sealing Methods 0.000 claims abstract description 10
- 230000001954 sterilising effect Effects 0.000 claims abstract description 10
- 238000012360 testing method Methods 0.000 claims abstract description 10
- 238000003756 stirring Methods 0.000 claims abstract description 8
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 6
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 6
- 230000000153 supplemental effect Effects 0.000 claims abstract description 6
- 230000001360 synchronised effect Effects 0.000 claims abstract description 6
- 210000002257 embryonic structure Anatomy 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims description 18
- 239000002699 waste material Substances 0.000 claims description 15
- 239000012530 fluid Substances 0.000 claims description 11
- 230000035807 sensation Effects 0.000 claims description 10
- 230000033001 locomotion Effects 0.000 claims description 8
- 238000010079 rubber tapping Methods 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 6
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 claims description 6
- 229940033663 thimerosal Drugs 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 230000005484 gravity Effects 0.000 claims description 5
- 238000009423 ventilation Methods 0.000 claims description 5
- 238000005260 corrosion Methods 0.000 claims description 4
- 230000007797 corrosion Effects 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 4
- 238000009434 installation Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 239000000853 adhesive Substances 0.000 claims description 3
- 230000001070 adhesive effect Effects 0.000 claims description 3
- 230000005540 biological transmission Effects 0.000 claims description 3
- 238000004587 chromatography analysis Methods 0.000 claims description 3
- 238000007689 inspection Methods 0.000 claims description 3
- 230000035800 maturation Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 230000003670 easy-to-clean Effects 0.000 claims description 2
- 239000011810 insulating material Substances 0.000 claims description 2
- 238000009413 insulation Methods 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 230000000903 blocking effect Effects 0.000 claims 1
- 238000006073 displacement reaction Methods 0.000 claims 1
- 238000009738 saturating Methods 0.000 claims 1
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 239000007921 spray Substances 0.000 description 4
- 241000700605 Viruses Species 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000007789 gas Substances 0.000 description 2
- 229960005486 vaccine Drugs 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241001269238 Data Species 0.000 description 1
- 241000790917 Dioxys <bee> Species 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 210000003711 chorioallantoic membrane Anatomy 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000003255 drug test Methods 0.000 description 1
- QVGXLLKOCUKJST-BJUDXGSMSA-N oxygen-15 atom Chemical compound [15O] QVGXLLKOCUKJST-BJUDXGSMSA-N 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
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Abstract
The present invention provides a kind of Multi-function experimental chick embryo culture detection means,Including with the culture detection cabin of sealing temperature separation door and computer control cabinet,There is the vertical dial for being driven by rotary tray motor and multiple overhead supports being uniformly housed inside,The perspective retaining frame of fixed multiple experiment chicken embryos is arranged at the bottom of each overhead support,Computer control cabinet automatically controls the carbon dioxide sensor inside culture detection cabin as requested,Oxygen concentration sensor,Temperature Humidity Sensor,Infrared radiation device,Infrared embryo temperature detector surveys matrix,Constant temperature decontamination apparatus,Ultraviolet sunlight irradiator,Automatic turning embryo device,Activity detector and oxygen supply system control component,Temperature and humidity control is carried out to the experiment chicken embryo in culture,Supplemental oxygen,Convert and stir position,Cleaning and sterilizing,It is infrared,Ultraviolet sunlight irradiates,It is automatic in incubation to realize the detection for testing chicken embryo activity and maturity and shown by display,The beneficial effects of the invention are as follows：A kind of Multi-function experimental chick embryo culture detection means is developed, solves the problem that experiment chicken embryo synchronous cultivate and detect automatically.
Description
Technical field
The present invention relates to a kind of Multi-function experimental chick embryo culture detection means.
Background technology
Because more and more more scientific experiments are required for using chick chorioallantoic membrane in the scientific researches such as medical science and biotechnology
To carry out the research of the inoculated and cultured such as virus, vaccine or drug test, therefore just need to cultivate large quantities of chicken embryos, test
The fostering requirement environment temperature of chicken embryo, ambient humidity, intensity of illumination, strict equal, Uniform Ventilation is good, in incubation daily
Flip-flop transition, direction and number must be accurately consistent；Conventional chick embryo culture method is all to observe temperature and humidity using artificial,
Heated, cooled, humidified by hand when not meeting condition of culture, and it is uneven to divulge information, thus bad guarantee chicken embryo
Constant temperature and humidity, manual operation also expend very big time and manpower all the time in incubation, turn out come experiment chicken embryo because
Environment nuance causes development degree different, and the success rate and high-quality rate so cultivated are just very low, does not adapt to thus work as
Preceding high-volume virus scientific experiment and the sample quality requirement increasingly refined, scientific experiment behaviour is have impact on to a certain extent
The quality and safety of work, the deep development of correlative study is hindered, in order to overcome above-mentioned difficulties, fundamentally solve experiment chicken embryo
Culture the problem of, research and develop that a kind of culture environment is exactly the same, and incubation is accurately consistent, and possess automatic detection experiment chicken embryo
Activity, the experimental chicken that the synchronous progress such as incubation, constant temperature cleaning chicken embryo are cultivated and detected automatically can be automatically controlled
Embryo culture detection means is just into inevitable and urgent need.
The content of the invention
To solve the above problems, it is an object of the invention to provide a kind of Multi-function experimental chick embryo culture detection means, use
Automatic detection automatic control technology, realize the automatic culture of experiment chicken embryo and carry out testing the inspection of chicken embryo activity and maturity automatically
Survey, solve the problem that experiment chicken embryo synchronous cultivate and detect automatically.
To achieve the above object, the technical scheme is that providing a kind of Multi-function experimental chick embryo culture detection means,
Including one, the culture with sealing temperature separation door detects cabin, and there is a vertical dial driven by rotary tray motor inside, vertical to turn
Disk can rotate freely motion, and its rotary speed can control, and multiple hangings for remaining balance are housed on vertical dial
Support, the perspective retaining frames that can fix multiple experiment chicken embryos are laid on each overhead support, one by sensor cluster, control
Keyboard processed, the computer control cabinet of display composition can automatically control the dioxy of installation inside culture detection cabin according to requirement of experiment
Change carbon sensor, oxygen concentration sensor, Temperature Humidity Sensor, infrared radiation device, infrared embryo temperature detector survey matrix, constant temperature decontamination apparatus,
Ultraviolet sunlight irradiator, automatic turning embryo device, activity detector and oxygen supply system control component, temperature control is carried out to experiment chicken embryo
Wet, supplemental oxygen is controlled, convert and stirs position, the irradiation of cleaning and sterilizing, infrared, ultraviolet sunlight, and it is automatic real in incubation
Detected referring now to chicken embryo activity and maturity, solve the problem that experiment chicken embryo synchronous cultivate and detect automatically.
The beneficial effects of the invention are as follows：A kind of Multi-function experimental chick embryo culture detection means is developed, using automatic detection
Automatic control technology, is carried out by temperature and humidity control, supplemental oxygen, convert and stirs position, cleaning and sterilizing, infrared, purple for experiment chicken embryo
Outer solar radiation, and automatic realize is detected for chicken embryo activity and maturity in incubation, solves the experiment more work(of chicken embryo
The problem that synchronously can be cultivated and detected automatically；Adapt to current high-volume virus and vaccine scientific experiment and increasingly refine
Sample quality requirement, ensure that scientific experiment operation quality and safety, advance the deep development of correlative study.
Brief description of the drawings
Accompanying drawing is the schematic diagram of structure of the present invention.
In figure：
1st, display 2, control keyboard 3, carbon dioxide sensor 4, oxygen concentration sensor 5, Temperature Humidity Sensor
6th, ultraviolet sunlight irradiator 7, underslung bearing 8, pole wheel 9, overhead support 10, bottom light-metering source
11st, colour filter 12, oxygen control valve 13, oxygen supply system 14, source of oxygen 15, air vent
16th, waste liquid bottle 17, tapping valve 18, infrared embryo temperature detector survey matrix 19, bottom light sensation matrix 20, culture detection cabin
21st, retaining frame 22, activity detector 23, support frame 24, liquid-collecting hopper 25, topping-up pump are had an X-rayed
26th, Moving caster 27, sealing temperature separation door 28, constant temperature decontamination apparatus 29, supplying bottle 30, fluid warmer
31st, rotary tray motor 32, groove push pedal 33, jet head 34, electromagnetic clutch 35, movable push shaft
36th, back-moving spring 37, automatic turning embryo device 38, horizontal push away pole 39, vertical dial 40, sensor cluster
41st, infrared radiation device 42, computer control cabinet.
Embodiment
The present invention is illustrated with reference to drawings and Examples.
As shown in drawings, the present invention provides a kind of Multi-function experimental chick embryo culture detection means, it is characterized in that：Including one
Culture with sealing temperature separation door 27 detects cabin 20, and culture detection cabin 20 is the interior table made by corrosion resistant insulation temperature insulating material
The seal hatch of the smooth sterilization easy to clean in face, sealing temperature separation door 27 is provided with the front in culture detection cabin 20, cabin is detected in culture
Moved on 20 rear wall equipped with one by can the rotating freely of driving of rotary tray motor 31 and can control the vertical of rotary speed
The miscellaneous part of rotating disk 39 and its top, multiple overhead supports 9 for remaining balance are equipped with vertical dial 39, are each hung
A perspective retaining frame 21 that can fix multiple experiment chicken embryos is laid on hanging bracket 9, overhead support 9 is by firm corrosion-resistant rigidity
Material makes bottom L-type, top is cross flexible connection frame, and the bottom L-type of overhead support 9 is a keep level installation
Ventilative latticed perspective retaining frame 21, perspective retaining frame 21 top lays experiment chicken embryo, and each chicken embryo of testing is ventilative latticed
Depending on fixed in retaining frame 21, while the both sides up and down for testing chicken embryo do not have any block to pass freely through single color detection light
Irradiation, the equidistantly distributed of overhead support 9 is arranged on by vertical dial 39 by the underslung bearing 7 at each top of overhead support 9
Edge, because the center of gravity of whole overhead support 9 is in the centre position of following perspective retaining frame 21, so each overhead support 9
With can also be allowed while same trajectories varying cyclically different locus perspective retaining frame 21 remain horizontality not
Become, pole 38 is pushed away with the horizontal of pole wheel 8 at both ends of the top of overhead support 9 equipped with a right-angled intersection, when vertical dial 39
When driving the position of automatic turning embryo device 37 of the overhead support 9 arrival in the culture detection left and right sidewall of cabin 20, automatic turning
Groove push pedal 32 on embryo device 37 groove driving pole wheel 8 and it is horizontal push away pole 38 tilt cause whole overhead support 9 with one
The angle tilt side of setting turns, and the experiment chicken embryo being placed in the perspective retaining frame 21 of the bottom of overhead support 9 is conveyed to regard to inclined side
The effect of upset experiment chicken embryo, when vertical dial 39 drives the overhead support 9 to leave the position of automatic turning embryo device 37, it is placed in
Experiment chicken embryo in the perspective retaining frame 21 of the bottom of overhead support 9 recovers normal horizontality again；On the left side in culture detection cabin 20
One automatic turning embryo device 37 is respectively installed on right side wall to complete experiment chicken embryo in the shift transformation in space and stir, automatic turning embryo device
37 are made up of groove push pedal 32, electromagnetic clutch 34, movable push shaft 35 and back-moving spring 36, are fixed on vertical dial 39 or so
It is the mechanical device for moving left and right and resetting under the driving of electromagnetic clutch 34 on the culture detection bulkhead of cabin 20 of both sides, when
During circuit controling electromagnetism 34 adhesive of clutch, the movable inside movement progress groove push pedal 32 of push shaft 35 overcomes back-moving spring 36 to act on
Power moves to close to the center of vertical dial 39, and the groove of groove push pedal 32 jacks up the pole wheel 8 on the top of overhead support 9 and horizontal pushes away branch
Bar 38 so that whole overhead support 9 supports the angle tilt for causing a setting, and the horizontal pole wheel 8 pushed away on pole 38 is along recessed
Groove yaw motion in groove push pedal 32, the experiment chicken embryo that bottom is placed in perspective retaining frame 21 are conveyed to upset in fact with regard to inclined side
Test the effect of chicken embryo, until pole wheel 8 and it is horizontal push away pole 38 and leave the groove curve in groove push pedal 32 when, be placed in hanging branch
Experiment chicken embryo in the perspective retaining frame 21 of the bottom of frame 9 recovers normal horizontality again, and vertical dial 39 often rotates a circle, all
Overhead support 9 has the chance of the chicken embryo of upset experiment twice, and when that need not overturn, electromagnetic clutch 34 powers off, back-moving spring 36
Act on the movable resilience of push shaft 35 to reset, drive whole groove push pedal 32 to be retracted away from vertical dial 39, the horizontal stroke on overhead support 9
Pushing away pole 38 can not kept being placed under overhead support 9 by the promotion of groove push pedal 32, whole overhead support 9 under gravity's center control
Experiment chicken embryo in the perspective retaining frame 21 in portion is all the time divided by horizontality will not be overturn；It is outside the top in culture detection cabin 20
One computer control cabinet 20, culture detection cabin 20 interior side be provided with carbon dioxide sensor 3, oxygen concentration sensor 4,
Temperature Humidity Sensor 5, one infrared radiation device 41 is installed on the left side of the culture detection inner top of cabin 20 experiment chicken embryo is carried out
Infra-red radiation, it is real that the ultraviolet simulated solar irradiation of sunlight irradiator 6 irradiation is installed on the right of the culture detection inner top of cabin 20
Chicken embryo is tested, has one infrared embryo temperature detector to survey matrix 18 and oxygen supply system 13 on the right of the culture detection inner bottom part of cabin 20,
There is a constant temperature decontamination apparatus 28 on the left side of the culture detection inner bottom part of cabin 20, and constant temperature decontamination apparatus 28 is arranged under culture detection cabin 20
Portion, it is liquid supply and the collection device for carrying out cleaning and sterilizing automatically to the experiment chicken embryo being placed in perspective retaining frame 21, by receiving
Liquid funnel 24, jet head 33, topping-up pump 25, fluid warmer 30, supplying bottle 29, tapping valve 17, waste liquid bottle 16 and infrared radiation
Device 41 forms, and an aperture area is arranged on the bottom surface in culture detection cabin 20 slightly larger than the liquid-collecting hopper 24 of the perspective area of retaining frame 21
On, liquid-collecting hopper 24 is connected in waste liquid bottle 16 by a tapping valve 17, is a hydrojet in the surface of liquid-collecting hopper 24
First 33, supplying bottle 29 is connected to by a topping-up pump 25 and fluid warmer 30, supplying bottle 29 can be changed not as requested
Same cleaning fluid, thimerosal or clear water is sprayed, and has certain temperature installed in the culture detection inner top left side of cabin 20
Infrared radiation device 41 infra-red radiation heating and drying are carried out to experiment chicken embryo, be placed in overhead support when vertical dial 39 drives
When experiment chicken embryo in the perspective retaining frame 21 of 9 bottoms is moved to directly over liquid-collecting hopper 24 between jet head 33, jet head 33
The cleaning fluid with certain temperature, thimerosal or clear water are entered to the experiment chicken embryo immediately below jet head 33 by topping-up pump 25
Row spray, sprays the waste liquid stayed and is collected into liquid-collecting hopper 24 and be sent to by tapping valve 17 in waste liquid bottle 16,
The infrared radiation device 41 on the culture detection inner top left side of cabin 20 carries out infra-red radiation heating and drying to experiment chicken embryo；Examined in culture
Surveying the centre of the inner bottom part of cabin 20 has an activity detector 22, and activity detector 22 is mounted in the culture detection inner bottom part of cabin 20
Between electronic detection circuit device, bottom side light source 10, support frame 23, colour filter 11 and bottom light sensation matrix are included from structure
19 4 parts, the top of support frame 23 are equipped with bottom side light source 10 and colour filter 11, therefore send specific single color detection light,
When having an X-rayed retaining frame 21 by 39 bottommost of vertical dial, single color detection light is to being placed in the experiment chicken embryo having an X-rayed in retaining frame 21
Irradiation, the single color detection light of transmission are detected by bottom light sensation matrix 19, and bottom light sensation matrix 19 is a kind of photosensitive for transmiting
The sensing element of sense, can obtain whether tested experiment chicken embryo survives, survives according to different luminous intensities and chromatography
The degree of number and maturation；Culture detection cabin 20 side wall on be provided with several through holes as oxygen control valve 12, source of oxygen 14,
Waste liquid bottle 16, the pipeline of various sensors and circuit are installed and used, and the bottom in culture detection cabin 20 is easy to equipped with Moving caster 26
It is mobile, also it is provided with the ventilation that air vent 15 ensures nacelle in the side wall in culture detection cabin 20；One by sensor cluster 40, control
The computer control cabinet 42 that keyboard 2, display 1 form can input setting running parameter according to requirement of experiment and automatically control culture inspection
Survey the lambda sensor 3 installed the inside of cabin 20, oxygen concentration sensor 4, Temperature Humidity Sensor 5, infrared radiation device 41, infrared embryo temperature detector
Survey matrix 18, constant temperature decontamination apparatus 28, ultraviolet sunlight irradiator 6, automatic turning embryo device 37, activity detector 22 and oxygen supply system
13 control elements, is carried out by temperature and humidity control, supplemental oxygen, convert and stirs position, cleaning and sterilizing, infrared, ultraviolet for experiment chicken embryo
Solar radiation, and automatic realize is detected for chicken embryo activity and maturity in incubation, and it is multi-functional to solve experiment chicken embryo
The synchronous problem cultivate and detect automatically.
Embodiment：
In use, getting out the large batch of fresh experiment chicken embryo of experiment first, selected according to experiment experiment chicken embryo used
The perspective retaining frame 21 of fixed correspondingly sized size and dimension, correspondingly sized size and shape are selected further according to selected perspective retaining frame 21
The overhead support 9 of shape, supplying bottle 29 is filled cleaning fluid either thimerosal or clear water by experimental requirement, by waste liquid bottle 16
Interior discharging of waste liquid is clean；The positive sealing temperature separation door 27 in culture detection cabin 20 is opened, perspective retaining frame 21 is put into overhead support 9
On, by the fresh top of perspective retaining frame 21 tested chicken embryo and be put on overhead support 9, each chicken embryo of testing is ventilative latticed
Depending on fixed in retaining frame 21, while the both sides up and down for testing chicken embryo do not have any block to pass freely through single color detection light
Irradiation, sealing temperature separation door 27 is closed, is turned on the power switch, computer control cabinet 42 is powered, will according to the experiment of culture experiment chicken embryo
Ask and detection is survived by the input temp of control keyboard 2, humidity, oxygen concentration, illumination, ventilation, the upset of experiment chicken embryo, cleaning, chicken embryo
Deng running parameter, culture detection cabin 20 starts working according to the running parameter of setting, and the display 1 of computer control cabinet 42 is real-time
Total data of the record display experiment chicken embryo 22 in incubation, equidistantly distributed are arranged on the edge of vertical dial 39
Several overhead supports 9 are because the center of gravity of whole overhead support 9 is in the centre position of following perspective retaining frame 21, so each hanging
Support 9 with can also be allowed while same trajectories varying cyclically different locus perspective retaining frame 21 remain horizontality
It is constant, when vertical dial 39 drives overhead support 9 to reach the automatic turning embryo device 37 being arranged in the culture detection left and right sidewall of cabin 20
Position when, the adhesive of circuit controling electromagnetism clutch 34, the movable inside movement progress groove push pedal 32 of push shaft 35 overcomes reset bullet
The active force of spring 36 moves to close to the center of vertical dial 39, and the groove of groove push pedal 32 jacks up the pole wheel 8 on the top of overhead support 9
Pole 38 is pushed away with horizontal so that whole overhead support 9 supports the angle tilt for causing a setting, the horizontal pole wheel pushed away on pole 38
8 along the groove yaw motion in groove push pedal 32, and the experiment chicken embryo that bottom is placed in perspective retaining frame 21 transfers with regard to inclined side
Upset experiment chicken embryo, when pole wheel 8 and it is horizontal push away pole 38 and leave the groove curve in groove push pedal 32 when, the bottom of overhead support 9
Perspective retaining frame 21 in experiment chicken embryo recover normal horizontality again, vertical dial 39 often rotates a circle, all hanging branch
Twice, such as testing chicken embryo need not overturn, and electromagnetic clutch 34 powers off, and back-moving spring 36 acts on for experiment chicken embryo upset on frame 9
Resetted in the movable resilience of push shaft 35, drive whole groove push pedal 32 to be retracted away from vertical dial 39, whole overhead support 9 can be in weight
Heart control keeps the experiment chicken embryo that is placed in the perspective retaining frame 21 of the bottom of overhead support 9 all the time down divided by horizontality will not be turned over
Turn；When vertical dial 39 drives the experiment chicken embryo being placed in the perspective retaining frame 21 of the bottom of overhead support 9 to be moved to liquid-collecting hopper
When directly over 24 between jet head 33, jet head 33 by topping-up pump 25 by the cleaning fluid with certain temperature, thimerosal or
Person's clear water sprays to the experiment chicken embryo immediately below jet head 33, sprays the waste liquid stayed and is collected into liquid-collecting hopper 24
It is sent to by tapping valve 17 in waste liquid bottle 16, the infrared radiation device 41 on the culture detection inner top left side of cabin 20 is to experimental chicken
Embryo carries out infra-red radiation heating and drying；When having an X-rayed retaining frame 21 by 39 bottommost of vertical dial, single color detection light is to peace
The experiment chicken embryo irradiation being placed in perspective retaining frame 21, the single color detection light of transmission are detected by bottom light sensation matrix 19, bottom light
Sense matrix 19 is a kind of sensing element for transmiting photaesthesia, can be tested according to different luminous intensities and chromatography
Experiment chicken embryo whether survive, the degree of the number that survives and maturation；Computer control cabinet 42 passes through carbon dioxide sensor at any time
3rd, oxygen concentration sensor 4, Temperature Humidity Sensor 5, sensor cluster 40, activity detector（22）, infrared embryo temperature detector survey matrix
18th, the sensor such as bottom light sensation matrix 19 detection culture detection cabin 20 inside temperature, humidity, gas concentration lwevel, oxygen concentration,
The real time datas such as illumination, ventilation, the upset of experiment chicken embryo, cleaning are simultaneously analyzed, then automatically control adjustment infrared radiation device 41,
Infrared embryo temperature detector surveys matrix 18, constant temperature decontamination apparatus 28, ultraviolet sunlight irradiator 6, automatic turning embryo device 37, activity detector 22 and oxygen
The control element of gas supply system 13, to experiment chicken embryo carry out temperature and humidity control, supplemental oxygen, convert and stir position, cleaning and sterilizing,
Infrared, ultraviolet sunlight irradiation, and carry out chicken embryo activity and maturity detection automatically in incubation, after the completion of experiment, close
Power switch, the positive sealing temperature separation door 27 in culture detection cabin 20 is opened, by the perspective on the overhead support 9 on vertical dial 39
Retaining frame 21 is fetched into the outside of culture detection cabin 20, then carries out cleaning and sterilizing to the inside of culture detection cabin 20, is closed after drying close
Envelope temperature separation door 27 prepares experiment next time and used, and cultured experiment chicken embryo is delivered into experiment in next step uses.
Claims (6)
1. a kind of Multi-function experimental chick embryo culture detection means, it is characterized in that：Including one with sealing temperature separation door（27）Training
Support detection cabin（20）, inside has one by rotary tray motor（31）The vertical dial of driving（39）, vertical dial（39）Can be free
Rotary motion, its rotary speed can control, in vertical dial（39）It is upper that multiple overhead supports for remaining balance are housed
（9）, each overhead support（9）It is upper to lay a perspective retaining frame that fix multiple experiment chicken embryos（21）, one by sensor
Component（40）, control keyboard（2）, display（1）The computer control cabinet of composition（42）Setting work can be inputted according to requirement of experiment
Make parameter automatically by detection cabin（20）The carbon dioxide sensor of inside installation（3）, oxygen concentration sensor（4）, humiture pass
Sensor（5）, sensor cluster（40）, activity detector（22）, infrared embryo temperature detector survey matrix（18）, bottom light sensation matrix（19）
Sensor controls infrared radiation device（41）, infrared embryo temperature detector survey matrix（18）, constant temperature decontamination apparatus（28）, ultraviolet sunlight irradiator
（6）, automatic turning embryo device（37）, activity detector（22）With oxygen supply system（13）Control element, experiment chicken embryo is controlled
Temperature control is wet, supplemental oxygen, converts and stirs position, the irradiation of cleaning and sterilizing, infrared, ultraviolet sunlight, and automatic in incubation
Realize and detected for chicken embryo activity and maturity solve the difficulty that experiment chicken embryo synchronous cultivate and detect automatically
Topic.
2. a kind of Multi-function experimental chick embryo culture detection means according to claim 1, it is characterized in that：The culture detection
Cabin（20）It is the seal hatch of the smooth sterilization easy to clean of inner surface made by corrosion resistant insulation temperature insulating material, is detected in culture
Cabin（20）Front be provided with sealing temperature separation door（27）, cabin is detected in culture（20）Rear wall on equipped with one by rotary tray motor（31）
Can rotating freely for driving is moved and can control the vertical dial of rotary speed（39）With the miscellaneous part of its top, training
Support detection cabin（20）Left and right sidewall on an automatic turning embryo device is respectively installed（37）Displacement of the experiment chicken embryo in space is completed to become
Change and stir, cabin is detected in culture（20）Top outside be a computer control cabinet（42）, cabin is detected in culture（20）It is interior
Portion side is provided with carbon dioxide sensor（3）, oxygen concentration sensor（4）, Temperature Humidity Sensor（5）, culture detection cabin（20）
The left side of inner top is provided with an infrared radiation device（41）Infra-red radiation is carried out to experiment chicken embryo, cabin is detected in culture（20）It is interior
One ultraviolet sunlight irradiator is installed on the right of top（6）Simulated solar irradiation irradiation experiment chicken embryo, cabin is detected in culture（20）
There is one infrared embryo temperature detector to survey matrix on the right of inner bottom part（18）With oxygen supply system（13）, cabin is detected in culture（20）Interior bottom
There is a constant temperature decontamination apparatus on the left side in portion（28）, cabin is detected in culture（20）There is an activity detector centre of inner bottom part
（22）, culture detection cabin（20）Side wall on be provided with several through holes as oxygen control valve（12）, source of oxygen（14）, waste liquid bottle
（16）, various sensors pipeline and circuit install and use, culture detection cabin（20）Bottom Moving caster is housed（26）It is easy to
It is mobile, culture detection cabin（20）Side wall on be also provided with air vent（15）Ensure the ventilation of nacelle.
3. a kind of Multi-function experimental chick embryo culture detection means according to claim 1, it is characterized in that：The activity detection
Device（22）It is mounted in culture detection cabin（20）Electronic detection circuit device among inner bottom part, includes bottom side light from structure
Source（10）, support frame（23）, colour filter（11）With bottom light sensation matrix（19）Four parts, support frame（23）Top bottom is housed
Side light source（10）And colour filter（11）, therefore specific single color detection light is sent, when perspective retaining frame（21）Pass through vertical dial
（39）During bottommost, single color detection light is to being placed in perspective retaining frame（21）Interior experiment chicken embryo irradiation, the single color inspection of transmission
Light-metering is by bottom light sensation matrix（19）Detection, bottom light sensation matrix（19）It is a kind of sensing element for transmiting photaesthesia, can
With according to different luminous intensities and chromatography obtain tested experiment chicken embryo whether survive, the journey of the number that survives and maturation
Degree.
4. a kind of Multi-function experimental chick embryo culture detection means according to claim 1, it is characterized in that：The overhead support
（9）Be bottom L-type is made by firm corrosion-resistant rigid material, top is cross flexible connection frame, overhead support（9）Under
Portion's L-type is the ventilative latticed perspective retaining frame of a keep level installation（21）, have an X-rayed retaining frame（21）Experiment chicken embryo is laid in top,
Each experiment chicken embryo is in ventilative latticed perspective retaining frame（21）It is interior fixed, while the both sides up and down for testing chicken embryo are not any
Single color detection light irradiation can be passed freely through by blocking, and pass through each overhead support（9）The underslung bearing at top（7）By hanging branch
Frame（9）Equidistantly distributed is arranged on vertical dial（39）Edge, due to whole overhead support（9）Center of gravity following saturating
Depending on retaining frame（21）Centre position, so each overhead support（9）With the different locus of same trajectories varying cyclically
It can also allow simultaneously and have an X-rayed retaining frame（21）Remain that horizontality is constant, in overhead support（9）Top one cross is housed
The both ends band pole of intersection is taken turns（8）Horizontal push away pole（38）, work as vertical dial（39）Drive the overhead support（9）Arrival is arranged on
Culture detection cabin（20）Automatic turning embryo device in left and right sidewall（37）Position when, automatic turning embryo device（37）On groove push pedal
（32）Groove driving pole wheel（8）Pole is pushed away with horizontal（38）Inclination causes whole overhead support（9）With the angle of a setting
Inclined side turns, and is placed in overhead support（9）The perspective retaining frame of bottom（21）Interior experiment chicken embryo is conveyed to upset in fact with regard to inclined side
The effect of chicken embryo is tested, works as vertical dial（39）Drive the overhead support（9）Leave automatic turning embryo device（37）Position when, be placed in
Overhead support（9）The perspective retaining frame of bottom（21）Interior experiment chicken embryo recovers normal horizontality again.
5. a kind of Multi-function experimental chick embryo culture detection means according to claim 1, it is characterized in that：The automatic turning embryo
Device（37）By groove push pedal（32）, electromagnetic clutch（34）, movable push shaft（35）And back-moving spring（36）Composition, is integrally fixed at
Vertical dial（39）The culture detection cabin of the left and right sides（20）In electromagnetic clutch on bulkhead（34）Driving under move left and right simultaneously
The mechanical device of reset, when circuit controling electromagnetism clutch（34）During adhesive, movable push shaft（35）Inside movement progress groove pushes away
Plate（32）Overcome back-moving spring（36）Active force is to close to vertical dial（39）Center is moved, groove push pedal（32）Groove jack up
Overhead support（9）The pole wheel on top（8）Pole is pushed away with horizontal（38）So that whole overhead support（9）Support causes a setting
Angle tilt, it is horizontal to push away pole（38）On pole wheel（8）Along groove push pedal（32）On groove yaw motion, bottom lay
In perspective retaining frame（21）Interior experiment chicken embryo is conveyed to the effect of upset experiment chicken embryo with regard to inclined side, until pole is taken turns（8）And horizontal stroke
Push away pole（38）Leave groove push pedal（32）On groove curve when, be placed in overhead support（9）The perspective retaining frame of bottom（21）
Interior experiment chicken embryo recovers normal horizontality, vertical dial again（39）Often rotate a circle, whole overhead supports（9）Have two
The chance of secondary upset experiment chicken embryo, the electromagnetic clutch when that need not overturn（34）Power-off, back-moving spring（36）Act on activity
Push shaft（35）Resilience resets, and drives whole groove push pedal（32）It is retracted away from vertical dial（39）, overhead support（9）On horizontal push away
Pole（38）Not by groove push pedal（32）Promotion, whole overhead support（9）It can keep being placed in hanging branch under gravity's center control
Frame（9）The perspective retaining frame of bottom（21）Interior experiment chicken embryo is all the time divided by horizontality will not be overturn.
6. a kind of Multi-function experimental chick embryo culture detection means according to claim 1, it is characterized in that：The constant temperature decontamination
Device（28）Cabin is detected installed in culture（20）Bottom, be to being placed in perspective retaining frame（21）Interior experiment chicken embryo carries out clear automatically
The liquid supply of wash disinfection and collection device, by liquid-collecting hopper（24）, jet head（33）, topping-up pump（25）, fluid warmer
（30）, supplying bottle（29）, tapping valve（17）, waste liquid bottle（16）With infrared radiation device（41）Composition, an aperture area are slightly larger than
Have an X-rayed retaining frame（21）The liquid-collecting hopper of area（24）Cabin is detected installed in culture（20）Bottom surface on, liquid-collecting hopper（24）Pass through one
Individual tapping valve（17）It is connected to waste liquid bottle（16）In, in liquid-collecting hopper（24）Surface be a jet head（33）, pass through one
Individual topping-up pump（25）And fluid warmer（30）It is connected to supplying bottle（29）, supplying bottle（29）It can change as requested different
Cleaning fluid, thimerosal or clear water are sprayed, and cabin is detected installed in culture（20）The inner top left side has certain temperature
Infrared radiation device（41）Infra-red radiation heating and drying are carried out to experiment chicken embryo, work as vertical dial（39）Drive is placed in hanging branch
Frame（9）The perspective retaining frame of bottom（21）Interior experiment chicken embryo is moved to liquid-collecting hopper（24）Surface and jet head（33）Between
When, jet head（33）Pass through topping-up pump（25）By the cleaning fluid with certain temperature, thimerosal or clear water to jet head（33）
The experiment chicken embryo of underface is sprayed, and is sprayed the waste liquid stayed and is collected into liquid-collecting hopper（24）In pass through tapping valve（17）
It is sent to waste liquid bottle（16）In, culture detection cabin（20）The infrared radiation device on the inner top left side（41）Experiment chicken embryo is carried out red
External radiation heats and drying.
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| CN106668888A (en) * | 2017-01-19 | 2017-05-17 | 新疆国际旅行卫生保健中心 | Disinfecting device for experimental surgical instruments |
| CN108986066A (en) * | 2018-04-23 | 2018-12-11 | 中国农业科学院特产研究所 | A kind of detection method and system of chicken embryo |
| CA3123314A1 (en) * | 2018-12-21 | 2020-06-25 | Octane Biotech Inc. | Carousel for modular biologic production units |
| CN109618981B (en) * | 2019-01-25 | 2021-07-27 | 天津大学 | A method for detecting the survival state of chicken embryos based on frequency characteristics of absorption spectrum |
| CN114752497A (en) * | 2022-06-16 | 2022-07-15 | 江苏中慧元通生物科技股份有限公司 | Sterile culture device for preparing influenza vaccine |
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