CN105200008A - Stem Cell Medium - Google Patents
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- CN105200008A CN105200008A CN201510717978.4A CN201510717978A CN105200008A CN 105200008 A CN105200008 A CN 105200008A CN 201510717978 A CN201510717978 A CN 201510717978A CN 105200008 A CN105200008 A CN 105200008A
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Abstract
本发明公开了一种干细胞培养基，包括改良DMEM/F12基础培养基和添加剂，所述添加剂包括碳酸氢钠、氨基酸螯合硒、重组人胰岛素生长因子、重组人碱性成纤维细胞生长因子、重组人乳铁蛋白、抗坏血酸、重组人血小板衍生生长因子、重组人血管内皮细胞生长因子、二十八烷醇、聚乙烯醇、聚乙烯吡咯烷酮、重组人表皮生长因子。本发明基能够快速扩增干细胞同时又不影响干细胞的潜能，干细胞的扩增速度较常规培养基提高3-5倍，而且能用于培养多种组织的干细胞，具有极佳的适用性，所培养的干细胞分化能力强，可分化成多种功能细胞，具有很高的科研和医学应用价值，培养基成分明确，品质稳定，使得培养的干细胞在移植后不容易产生人体排斥反应。The invention discloses a stem cell culture medium, which comprises improved DMEM/F12 basal medium and additives, and the additives include sodium bicarbonate, amino acid chelated selenium, recombinant human insulin growth factor, recombinant human basic fibroblast growth factor, Recombinant human lactoferrin, ascorbic acid, recombinant human platelet-derived growth factor, recombinant human vascular endothelial growth factor, octacosanol, polyvinyl alcohol, polyvinylpyrrolidone, recombinant human epidermal growth factor. The base of the invention can rapidly expand stem cells without affecting the potential of stem cells, and the expansion speed of stem cells is 3-5 times higher than that of conventional medium, and can be used to cultivate stem cells of various tissues, and has excellent applicability. The cultured stem cells have strong differentiation ability and can be differentiated into a variety of functional cells. They have high scientific research and medical application value. The medium composition is clear and the quality is stable, so that the cultured stem cells are not easy to produce human rejection after transplantation.
Description
技术领域technical field
本发明涉及及干细胞培养技术领域，具体涉及一种干细胞培养基。The invention relates to the technical field of stem cell culture, in particular to a stem cell culture medium.
背景技术Background technique
诸多研究表明干细胞不仅可以自我更新，在条件合适的情况下能分化成其他功能细胞，因此干细胞有望成为治疗人类疑难疾病的有效手段。然而，干细胞在正常成体组织中含量甚微，在体外如何快速扩增与培养干细胞培养是研究干细胞的作用机制及探索其在治疗人类疾病治疗方法的重要技术。Many studies have shown that stem cells can not only self-renew, but also differentiate into other functional cells under suitable conditions. Therefore, stem cells are expected to become an effective means of treating difficult human diseases. However, the content of stem cells in normal adult tissues is very small. How to rapidly expand and cultivate stem cells in vitro is an important technology for studying the mechanism of stem cells and exploring their therapeutic methods in the treatment of human diseases.
干细胞尽量含量甚微，但广泛分布于哺乳动物的各个组织器官，这些组织或器官包括但不局限于骨髓、脐带、脂肪组织、脑组织、视网膜、心脏、肝脏、肺以及皮肤等。大量研究表明，这些低含量的干细胞在体外可以通过适当的方法进行进一步扩增，最常规的一种干细胞培养基就是添加有10％胎牛血清的培养基。然而，采用这种培养基培养的干细胞，不仅生长比较缓慢，而且传递次数超过5代之后其分化成功能细胞的潜能大大降低。The content of stem cells is as small as possible, but they are widely distributed in various tissues and organs of mammals, including but not limited to bone marrow, umbilical cord, adipose tissue, brain tissue, retina, heart, liver, lung and skin. A large number of studies have shown that these low-content stem cells can be further expanded in vitro by appropriate methods. The most conventional stem cell culture medium is the medium supplemented with 10% fetal bovine serum. However, stem cells cultured in this medium not only grow slowly, but also have a greatly reduced potential to differentiate into functional cells after more than 5 passages.
发明内容Contents of the invention
为解决上述问题，本发明提供了一种干细胞培养基。To solve the above problems, the present invention provides a stem cell culture medium.
为实现上述目的，本发明采取的技术方案为：In order to achieve the above object, the technical scheme that the present invention takes is:
干细胞培养基，包括改良DMEM/F12基础培养基和添加剂，所述添加剂包括Stem cell culture medium, including improved DMEM/F12 basal medium and additives, said additives include
碳酸氢钠400-600μg/ml、氨基酸螯合硒8-20ng/ml、重组人胰岛素生长因子10-30ng/ml、重组人碱性成纤维细胞生长因子80-120ng/ml、重组人乳铁蛋白7-13μg/ml、抗坏血酸50-70μg/ml、重组人血小板衍生生长因子3-7μg/ml、重组人血管内皮细胞生长因子1-7ng/ml、二十八烷醇0.1-10μg/ml、聚乙烯醇0.1-10μg/ml、聚乙烯吡咯烷酮0.1-10μg/ml、重组人表皮生长因子1-10μg/ml。Sodium bicarbonate 400-600μg/ml, amino acid chelated selenium 8-20ng/ml, recombinant human insulin growth factor 10-30ng/ml, recombinant human basic fibroblast growth factor 80-120ng/ml, recombinant human lactoferrin 7-13μg/ml, ascorbic acid 50-70μg/ml, recombinant human platelet-derived growth factor 3-7μg/ml, recombinant human vascular endothelial growth factor 1-7ng/ml, octacosanol 0.1-10μg/ml, poly Vinyl alcohol 0.1-10 μg/ml, polyvinylpyrrolidone 0.1-10 μg/ml, recombinant human epidermal growth factor 1-10 μg/ml.
优选地，所述改良DMEM/F12基础培养基包括DMEM/F12基础培养、维生素和营养元素。Preferably, the improved DMEM/F12 basal medium includes DMEM/F12 basal culture, vitamins and nutrient elements.
优选地，所述维生素包括维生素C1-50μg/ml，维生素B族30-100μg/ml，维生素E10-100μg/mL。Preferably, the vitamins include 1-50 μg/ml of vitamin C, 30-100 μg/ml of vitamin B family, and 10-100 μg/mL of vitamin E.
优选地，所述营养元素包括L-谷氨酰胺1-5mg/ml，蛋氨酸锰1-10mg/ml，氯化锌1-5mg/ml、氨基酸螯合镁1-5mg/ml、硒蛋氨酸1.5-5.5mg/ml、植酸酶0.5-0.75mg/ml、吡啶酸铬0.055-0.065mg/ml、碘酸钙0.1-0.3mg/ml、赖氨酸1-6mg/ml、三氯化六氨合钴0.05-0.065mg/ml、蛋白锗0.55-0.75mg/ml、碘酵母2-6mg/ml、氯化铜0.1-1mg/ml。Preferably, the nutritional elements include L-glutamine 1-5mg/ml, manganese methionine 1-10mg/ml, zinc chloride 1-5mg/ml, amino acid chelated magnesium 1-5mg/ml, selenomethionine 1.5- 5.5mg/ml, phytase 0.5-0.75mg/ml, chromium picolinate 0.055-0.065mg/ml, calcium iodate 0.1-0.3mg/ml, lysine 1-6mg/ml, hexaamine trichloride Cobalt 0.05-0.065mg/ml, protein germanium 0.55-0.75mg/ml, iodine yeast 2-6mg/ml, copper chloride 0.1-1mg/ml.
优选地，所述添加剂包括碳酸氢钠400μg/ml、氨基酸螯合硒8ng/ml、重组人胰岛素生长因子10ng/ml、重组人碱性成纤维细胞生长因子80ng/ml、重组人乳铁蛋白7μg/ml、抗坏血酸50μg/ml、重组人血小板衍生生长因子3μg/ml、重组人血管内皮细胞生长因子1ng/ml、二十八烷醇0.1μg/ml、聚乙烯醇0.1μg/ml、聚乙烯吡咯烷酮0.1μg/ml、重组人表皮生长因子1μg/ml。Preferably, the additives include sodium bicarbonate 400 μg/ml, amino acid chelated selenium 8 ng/ml, recombinant human insulin growth factor 10 ng/ml, recombinant human basic fibroblast growth factor 80 ng/ml, recombinant human lactoferrin 7 μg /ml, ascorbic acid 50μg/ml, recombinant human platelet-derived growth factor 3μg/ml, recombinant human vascular endothelial growth factor 1ng/ml, octacosanol 0.1μg/ml, polyvinyl alcohol 0.1μg/ml, polyvinylpyrrolidone 0.1 μg/ml, recombinant human epidermal growth factor 1 μg/ml.
优选地，所述添加剂包括碳酸氢钠500μg/ml、氨基酸螯合硒14ng/ml、重组人胰岛素生长因子20ng/ml、重组人碱性成纤维细胞生长因子100ng/ml、重组人乳铁蛋白10μg/ml、抗坏血酸60μg/ml、重组人血小板衍生生长因子5μg/ml、重组人血管内皮细胞生长因子4ng/ml、二十八烷醇5.05μg/ml、聚乙烯醇5.05μg/ml、聚乙烯吡咯烷酮5.05μg/ml、重组人表皮生长因子5.5μg/ml。Preferably, the additives include sodium bicarbonate 500 μg/ml, amino acid chelated selenium 14 ng/ml, recombinant human insulin growth factor 20 ng/ml, recombinant human basic fibroblast growth factor 100 ng/ml, recombinant human lactoferrin 10 μg /ml, ascorbic acid 60μg/ml, recombinant human platelet-derived growth factor 5μg/ml, recombinant human vascular endothelial growth factor 4ng/ml, octacosanol 5.05μg/ml, polyvinyl alcohol 5.05μg/ml, polyvinylpyrrolidone 5.05μg/ml, recombinant human epidermal growth factor 5.5μg/ml.
优选地，所述添加剂包括碳酸氢钠600μg/ml、氨基酸螯合硒20ng/ml、重组人胰岛素生长因子30ng/ml、重组人碱性成纤维细胞生长因子120ng/ml、重组人乳铁蛋白13μg/ml、抗坏血酸70μg/ml、重组人血小板衍生生长因子7μg/ml、重组人血管内皮细胞生长因子7ng/ml、二十八烷醇10μg/ml、聚乙烯醇10μg/ml、聚乙烯吡咯烷酮10μg/ml、重组人表皮生长因10μg/ml。Preferably, the additives include sodium bicarbonate 600 μg/ml, amino acid chelated selenium 20 ng/ml, recombinant human insulin growth factor 30 ng/ml, recombinant human basic fibroblast growth factor 120 ng/ml, recombinant human lactoferrin 13 μg /ml, ascorbic acid 70μg/ml, recombinant human platelet-derived growth factor 7μg/ml, recombinant human vascular endothelial growth factor 7ng/ml, octacosanol 10μg/ml, polyvinyl alcohol 10μg/ml, polyvinylpyrrolidone 10μg/ml ml, recombinant human epidermal growth factor 10 μg/ml.
本发明具有以下有益效果：The present invention has the following beneficial effects:
基能够快速扩增干细胞同时又不影响干细胞的潜能，干细胞的扩增速度较常规培养基提高3-5倍，而且能用于培养多种组织的干细胞，具有极佳的适用性，所培养的干细胞分化能力强，可分化成多种功能细胞，具有很高的科研和医学应用价值，培养基成分明确，品质稳定，使得培养的干细胞在移植后不容易产生人体排斥反应。The medium can quickly expand stem cells without affecting the potential of stem cells. The expansion speed of stem cells is 3-5 times higher than that of conventional medium, and it can be used to cultivate stem cells in various tissues. It has excellent applicability. The cultured Stem cells have strong differentiation ability and can be differentiated into a variety of functional cells. They have high scientific research and medical application value. The medium composition is clear and the quality is stable, so that the cultured stem cells are not easy to produce human rejection after transplantation.
具体实施方式Detailed ways
为了使本发明的目的及优点更加清楚明白，以下结合实施例对本发明进行进一步详细说明。应当理解，此处所描述的具体实施例仅仅用以解释本发明，并不用于限定本发明。In order to make the objects and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
实施例1Example 1
一种干细胞培养基，包括DMEM/F12基础培养、维生素C1μg/ml，维生素B族30μg/ml，维生素E10μg/mL、L-谷氨酰胺1mg/ml，蛋氨酸锰1mg/ml，氯化锌1mg/ml、氨基酸螯合镁1mg/ml、硒蛋氨酸1.5mg/ml、植酸酶0.5mg/ml、吡啶酸铬0.055mg/ml、碘酸钙0.1mg/ml、赖氨酸1mg/ml、三氯化六氨合钴0.05mg/ml、蛋白锗0.55mg/ml、碘酵母2mg/ml、氯化铜0.1mg/ml、碳酸氢钠400μg/ml、氨基酸螯合硒8ng/ml、重组人胰岛素生长因子10ng/ml、重组人碱性成纤维细胞生长因子80ng/ml、重组人乳铁蛋白7μg/ml、抗坏血酸50μg/ml、重组人血小板衍生生长因子3μg/ml、重组人血管内皮细胞生长因子1ng/ml、二十八烷醇0.1μg/ml、聚乙烯醇0.1μg/ml、聚乙烯吡咯烷酮0.1μg/ml、重组人表皮生长因子1μg/ml。A stem cell culture medium, including DMEM/F12 basal culture, vitamin C 1μg/ml, vitamin B family 30μg/ml, vitamin E 10μg/mL, L-glutamine 1mg/ml, manganese methionine 1mg/ml, zinc chloride 1mg/ml ml, amino acid chelated magnesium 1mg/ml, selenomethionine 1.5mg/ml, phytase 0.5mg/ml, chromium picolinate 0.055mg/ml, calcium iodate 0.1mg/ml, lysine 1mg/ml, trichloro Hexaammine cobalt 0.05mg/ml, protein germanium 0.55mg/ml, iodine yeast 2mg/ml, copper chloride 0.1mg/ml, sodium bicarbonate 400μg/ml, amino acid chelated selenium 8ng/ml, recombinant human insulin growth Factor 10ng/ml, recombinant human basic fibroblast growth factor 80ng/ml, recombinant human lactoferrin 7μg/ml, ascorbic acid 50μg/ml, recombinant human platelet-derived growth factor 3μg/ml, recombinant human vascular endothelial growth factor 1ng /ml, octacosanol 0.1 μg/ml, polyvinyl alcohol 0.1 μg/ml, polyvinylpyrrolidone 0.1 μg/ml, recombinant human epidermal growth factor 1 μg/ml.
实施例2Example 2
一种干细胞培养基，包括DMEM/F12基础培养、维生素C50μg/ml，维生素B族100μg/ml，维生素E100μg/mL、L-谷氨酰胺5mg/ml，蛋氨酸锰10mg/ml，氯化锌5mg/ml、氨基酸螯合镁5mg/ml、硒蛋氨酸5.5mg/ml、植酸酶0.75mg/ml、吡啶酸铬0.065mg/ml、碘酸钙0.3mg/ml、赖氨酸6mg/ml、三氯化六氨合钴0.065mg/ml、蛋白锗0.75mg/ml、碘酵母6mg/ml、氯化铜1mg/ml、碳酸氢钠500μg/ml、氨基酸螯合硒14ng/ml、重组人胰岛素生长因子20ng/ml、重组人碱性成纤维细胞生长因子100ng/ml、重组人乳铁蛋白10μg/ml、抗坏血酸60μg/ml、重组人血小板衍生生长因子5μg/ml、重组人血管内皮细胞生长因子4ng/ml、二十八烷醇5.05μg/ml、聚乙烯醇5.05μg/ml、聚乙烯吡咯烷酮5.05μg/ml、重组人表皮生长因子5.5μg/ml。A stem cell culture medium, including DMEM/F12 basal culture, vitamin C50μg/ml, vitamin B family 100μg/ml, vitamin E100μg/mL, L-glutamine 5mg/ml, manganese methionine 10mg/ml, zinc chloride 5mg/ml ml, amino acid chelated magnesium 5mg/ml, selenomethionine 5.5mg/ml, phytase 0.75mg/ml, chromium picolinate 0.065mg/ml, calcium iodate 0.3mg/ml, lysine 6mg/ml, trichlor Hexaammine cobalt 0.065mg/ml, protein germanium 0.75mg/ml, iodine yeast 6mg/ml, copper chloride 1mg/ml, sodium bicarbonate 500μg/ml, amino acid chelated selenium 14ng/ml, recombinant human insulin growth factor 20ng/ml, recombinant human basic fibroblast growth factor 100ng/ml, recombinant human lactoferrin 10μg/ml, ascorbic acid 60μg/ml, recombinant human platelet-derived growth factor 5μg/ml, recombinant human vascular endothelial growth factor 4ng/ml ml, octacosanol 5.05 μg/ml, polyvinyl alcohol 5.05 μg/ml, polyvinylpyrrolidone 5.05 μg/ml, recombinant human epidermal growth factor 5.5 μg/ml.
实施例3Example 3
一种干细胞培养基，包括DMEM/F12基础培养、维生素C25.5μg/ml，维生素B族65μg/ml，维生素E55μg/mL、L-谷氨酰胺3mg/ml，蛋氨酸锰5.5mg/ml，氯化锌3mg/ml、氨基酸螯合镁3mg/ml、硒蛋氨酸3.5mg/ml、植酸酶0.625mg/ml、吡啶酸铬0.06mg/ml、碘酸钙0.2mg/ml、赖氨酸3.5mg/ml、三氯化六氨合钴0.0575mg/ml、蛋白锗0.65mg/ml、碘酵母4mg/ml、氯化铜0.55mg/ml、碳酸氢钠600μg/ml、氨基酸螯合硒20ng/ml、重组人胰岛素生长因子30ng/ml、重组人碱性成纤维细胞生长因子120ng/ml、重组人乳铁蛋白13μg/ml、抗坏血酸70μg/ml、重组人血小板衍生生长因子7μg/ml、重组人血管内皮细胞生长因子7ng/ml、二十八烷醇10μg/ml、聚乙烯醇10μg/ml、聚乙烯吡咯烷酮10μg/ml、重组人表皮生长因10μg/ml。A stem cell medium, including DMEM/F12 basal culture, vitamin C25.5μg/ml, vitamin B family 65μg/ml, vitamin E55μg/ml, L-glutamine 3mg/ml, manganese methionine 5.5mg/ml, chloride Zinc 3mg/ml, amino acid chelated magnesium 3mg/ml, selenomethionine 3.5mg/ml, phytase 0.625mg/ml, chromium picolinate 0.06mg/ml, calcium iodate 0.2mg/ml, lysine 3.5mg/ml ml, hexaammine cobalt trichloride 0.0575mg/ml, protein germanium 0.65mg/ml, iodine yeast 4mg/ml, copper chloride 0.55mg/ml, sodium bicarbonate 600μg/ml, amino acid chelated selenium 20ng/ml, Recombinant human insulin growth factor 30ng/ml, recombinant human basic fibroblast growth factor 120ng/ml, recombinant human lactoferrin 13μg/ml, ascorbic acid 70μg/ml, recombinant human platelet-derived growth factor 7μg/ml, recombinant human vascular endothelium Cell growth factor 7ng/ml, octacosanol 10μg/ml, polyvinyl alcohol 10μg/ml, polyvinylpyrrolidone 10μg/ml, recombinant human epidermal growth factor 10μg/ml.
以上所述仅是本发明的优选实施方式，应当指出，对于本技术领域的普通技术人员来说，在不脱离本发明原理的前提下，还可以作出若干改进和润饰，这些改进和润饰也应视为本发明的保护范围。The above is only a preferred embodiment of the present invention, it should be pointed out that for those of ordinary skill in the art, without departing from the principle of the present invention, some improvements and modifications can also be made, and these improvements and modifications should also be It is regarded as the protection scope of the present invention.
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106148275A (en) * | 2016-08-08 | 2016-11-23 | 安徽惠恩生物科技股份有限公司 | A kind of culture medium improving the adherent property of epidermal stem cells |
| CN106282114A (en) * | 2016-08-08 | 2017-01-04 | 安徽惠恩生物科技股份有限公司 | A kind of fast breeding nerve stem cell culture medium |
| CN106834220A (en) * | 2016-12-24 | 2017-06-13 | 叶宗耀 | A kind of serum-free cultured chondrocytes base and preparation method thereof |
| CN107164325A (en) * | 2017-06-28 | 2017-09-15 | 北京再生生物科技研究院有限公司 | The preparation method and kit of the oligodendroglia in MSCs sources |
| CN107653222A (en) * | 2016-07-25 | 2018-02-02 | 赵玉明 | A kind of collocation method of sterile liquid cell culture medium |
| CN107686827A (en) * | 2017-10-30 | 2018-02-13 | 洛阳轩智生物科技有限公司 | Human adipose-derived stem cell special media |
| CN108699519A (en) * | 2016-01-12 | 2018-10-23 | 康干细胞生物技术有限公司 | The stem cell-derived allochthon containing the raised growth factor |
| CN111206017A (en) * | 2019-04-30 | 2020-05-29 | 浙江大学 | A kind of stem cell serum-free medium and its application |
| CN113166723A (en) * | 2018-12-07 | 2021-07-23 | 关东化学株式会社 | Undifferentiated maintenance medium for pluripotent stem cells |
| JPWO2021220731A1 (en) * | 2020-04-30 | 2021-11-04 | ||
| WO2021227573A1 (en) * | 2020-05-14 | 2021-11-18 | 梦芊科技知识产权有限公司 | Xeno-free culture medium and method for expansion of mesenchymal stem cells by means of using same |
| CN114134108A (en) * | 2021-11-30 | 2022-03-04 | 武汉伯韬生物科技有限公司 | Stem cell bioactive composition and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130071932A1 (en) * | 2010-03-23 | 2013-03-21 | Public University Corporation Yokohama City University | Culture method for causing differentiation of pluripotent mammalian cells |
| CN103243070A (en) * | 2013-04-25 | 2013-08-14 | 刘小青 | Stem cell medium and application thereof |
| CN103952374A (en) * | 2014-05-07 | 2014-07-30 | 北京赛贝生物技术有限公司 | Human pluripotent stem cell culture medium and culture method of human pluripotent stem cells |
| CN104357379A (en) * | 2014-09-30 | 2015-02-18 | 刘兴宇 | Stem cell culture medium |
-
2015
- 2015-10-23 CN CN201510717978.4A patent/CN105200008A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130071932A1 (en) * | 2010-03-23 | 2013-03-21 | Public University Corporation Yokohama City University | Culture method for causing differentiation of pluripotent mammalian cells |
| CN103243070A (en) * | 2013-04-25 | 2013-08-14 | 刘小青 | Stem cell medium and application thereof |
| CN103952374A (en) * | 2014-05-07 | 2014-07-30 | 北京赛贝生物技术有限公司 | Human pluripotent stem cell culture medium and culture method of human pluripotent stem cells |
| CN104357379A (en) * | 2014-09-30 | 2015-02-18 | 刘兴宇 | Stem cell culture medium |
Non-Patent Citations (2)
| Title |
|---|
| O. KILIAN ET.AL.: "EFFECTS OF PLATELET GROWTH FACTORS ON HUMAN MESENCHYMAL STEM CELLS AND HUMAN ENDOTHELIAL CELLS IN VITRO", 《EUROPEAN JOURNAL OF MEDICAL RESEARCH》 * |
| 李丽娜等: "人脐带间充质干细胞条件培养基体外支持造血的功能", 《中国实验血液学杂志》 * |
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108699519A (en) * | 2016-01-12 | 2018-10-23 | 康干细胞生物技术有限公司 | The stem cell-derived allochthon containing the raised growth factor |
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| CN106282114A (en) * | 2016-08-08 | 2017-01-04 | 安徽惠恩生物科技股份有限公司 | A kind of fast breeding nerve stem cell culture medium |
| CN106148275A (en) * | 2016-08-08 | 2016-11-23 | 安徽惠恩生物科技股份有限公司 | A kind of culture medium improving the adherent property of epidermal stem cells |
| CN106834220A (en) * | 2016-12-24 | 2017-06-13 | 叶宗耀 | A kind of serum-free cultured chondrocytes base and preparation method thereof |
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| CN107686827A (en) * | 2017-10-30 | 2018-02-13 | 洛阳轩智生物科技有限公司 | Human adipose-derived stem cell special media |
| CN107686827B (en) * | 2017-10-30 | 2018-11-16 | 集钧（上海）医疗科技发展有限公司 | Human adipose-derived stem cell special media |
| CN113166723B (en) * | 2018-12-07 | 2024-08-30 | 关东化学株式会社 | Undifferentiated maintenance medium for pluripotent stem cells |
| CN113166723A (en) * | 2018-12-07 | 2021-07-23 | 关东化学株式会社 | Undifferentiated maintenance medium for pluripotent stem cells |
| CN111206017A (en) * | 2019-04-30 | 2020-05-29 | 浙江大学 | A kind of stem cell serum-free medium and its application |
| CN111206017B (en) * | 2019-04-30 | 2022-02-18 | 浙江大学 | A kind of stem cell serum-free medium and its application |
| WO2021220731A1 (en) * | 2020-04-30 | 2021-11-04 | オリエンタル酵母工業株式会社 | Stem cell medium and stem cell culturing method |
| JPWO2021220731A1 (en) * | 2020-04-30 | 2021-11-04 | ||
| JP7633998B2 (en) | 2020-04-30 | 2025-02-20 | オリエンタル酵母工業株式会社 | Stem cell culture medium and method for culturing stem cells |
| WO2021227573A1 (en) * | 2020-05-14 | 2021-11-18 | 梦芊科技知识产权有限公司 | Xeno-free culture medium and method for expansion of mesenchymal stem cells by means of using same |
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