CN104815454A - Rosemary natural extract, bacteriostatic soup prepared from rosemary natural extract, and preparation method and use of bacteriostatic soup - Google Patents

Abstract

The invention provides rosemary natural extracts and uses the extracts to prepare bacteriostatic soap and its preparation method and application, so as to overcome the defects of chemically synthesized bacteriostatic agents. The scented soap of the present invention can not only deeply clean the skin, but also has a good antibacterial effect, has a good and long-lasting ability to remove common pathogenic bacteria, and has a certain maintenance effect on the skin, and is especially suitable for children and women, as well as the body People with weak resistance.

Description

Rosemary natural extract and use of the extract to prepare antibacterial soap and its preparation method and application

technical field

The invention relates to a novel scented soap, in particular to a natural extract of rosemary and the preparation method and application of the antibacterial scented soap by using the extract.

Background technique

There are a lot of pathogenic bacteria and fungi in the environment we live in, and we may get infected if we are not careful, and the skin is the main site where these pathogenic bacteria parasite. Using soap with antibacterial effect is one of the good measures to prevent infection. Most of the soaps sold on the market use chemical substances such as triclocarban and triclosan to inhibit bacteria. Their safety needs to be explored, and long-term use of certain chemical substances to inhibit bacterial growth will lead to drug resistance of the bacteria. And some manufacturers use sodium fatty acid and other surfactants as the main raw materials to make soaps, add quality improvers and appearance improvers, and make products after processing and molding. Because these pH values are high, the skin cannot tolerate them for a long time. Therefore, it can only be used to wash clothes. The pH value of plant weak base soap is low, and human skin can accept it for a long time, and various nutrients contained in plants also have the effect of caring for the skin. The natural antibacterial active substances contained in some plant extracts will not cause adverse reactions and side effects to the human body, and will not damage the health of users.

Contents of the invention

In order to solve the deficiencies in the prior art, the present invention provides rosemary natural extracts and uses the extracts to prepare bacteriostatic soap and its preparation method and application, so as to overcome the defects of chemically synthesized bacteriostatic agents.

The technical solution of the present invention is: a natural extract of rosemary, which is prepared according to the following steps: take 50 kilograms of rosemary dry samples, grind the rosemary dry samples into powder, soak 24h with 20 times the amount of 80% ethanol , and then filter with a Buchner funnel to collect the filtrate; repeat this operation until the ethanol soaking solution is colorless, and combine the filtrate; the filtrate is distilled under reduced pressure with a rotary evaporator, and the filtrate is concentrated to obtain a crude extract of rosemary; Adsorbed by adsorption resin to remove impurities such as chlorophyll to obtain about 10 kg of fat-soluble rosemary extract.

Another object of the present invention is to provide an antibacterial soap, the antibacterial activity and aromatic components in the antibacterial soap are the natural extract of rosemary as mentioned above.

As a further improved technical solution, the ingredients of the antibacterial soap include 5-10 parts of rosemary extract, 10-12 parts of coconut oil, 20-23 parts of soybean oil, 20-35 parts of sunflower oil, 8.5-9 parts of sodium hydroxide, 20-25 parts of water.

And another optimized technical scheme, the antibacterial soap is made of 5-10 parts of rosemary extract, 10-12 parts of coconut oil, 20-23 parts of soybean oil, 20-35 parts of sunflower oil, hydrogenated It consists of 8.5-9 parts of sodium and 20-25 parts of water.

The present invention also provides a kind of preparation method of antibacterial soap as above, and its step comprises: the first step: put coconut oil, soybean oil, sunflower seed oil into heat-resistant container in proportion according to weight, heat with water insulation The method is boiled to dissolve, so that the temperature of the oil is maintained at 35 ° C ~ 40 ° C; the second step: according to the required amount of water and sodium hydroxide, put the water into a glass container, and then slowly add sodium hydroxide, while adding Stir quickly with an alkali-resistant stirrer, completely dissolve the sodium hydroxide in water and cool to 35°C to 40°C to obtain an alkali solution; the third step: slowly pour the alkali solution in the second step into the first step and mix it Put the oil in a glass container, while adding it, stir continuously with an alkali-resistant stirrer for about 40 minutes to saponify the two until the two solutions are completely mixed to obtain a soap base, and adjust the pH to 7.5-8; the fourth step: go to the first step Add rosemary extract in proportion to the three-step soap base while stirring, and then stir evenly to obtain a mixed soap liquid; step five: use a brush dipped in a small amount of olive oil to paint the inside of the mold, and then mix the fourth step Pour the soap solution into the mold and cover the mold with a plastic film, and place it in a 35°C incubator for 2 to 3 days to obtain soap; Step 6: Take the soap prepared in Step 5 out of the mold and put it in a ventilated place to let it dry. It is dried naturally to obtain finished soap.

The present invention also provides a kind of antibacterial soap as above, the antibacterial soap is effective in inhibiting Clostridium perfringens, Staphylococcus aureus, Bacillus anthracis, Salmonella, Mycobacterium tuberculosis, Streptococcus A, Streptococcus pneumoniae, Application of Shigella dysenteriae or Shigella sonnei.

Carnosol, rosmarinic acid, rosmanol and other active ingredients contained in rosemary extract are effective against Clostridium perfringens, Staphylococcus aureus, Bacillus anthracis, Salmonella, Mycobacterium tuberculosis, chain A Bacillus, Streptococcus pneumoniae, Shigella dysenteriae, and Shigella sonnei all had different degrees of antibacterial effect.

Advantages of the present invention:

The product provided by the invention has significantly improved antibacterial activity and can effectively inhibit the growth of various pathogenic bacteria.

The production process of the product of the invention is simple and safe, and the antibacterial active substances and soap-based raw materials used are all natural products, and have the advantages of safety, non-toxicity and no side effects. Determine the active ingredient dosage. Because the content of natural antibacterial active ingredients is too large, it will affect the emulsification effect of soap, and if the amount is too small, it will not be able to achieve the antibacterial effect. After a long period of research and analysis of a large number of experimental data, the applicant has determined the appropriate dosage of the active ingredient so that it has a good antibacterial effect without affecting the molding of the soap.

Detailed ways

The present invention will be described in detail below in conjunction with the embodiments.

Example 1:

The preparation steps of rosemary extract: Weigh 50 kg of rosemary dry sample, grind the rosemary dry sample into powder, soak with 20 times the amount of 80% ethanol for 24 hours, then filter with Buchner funnel, and collect the filtrate. Repeat this operation until the ethanol soaking solution is colorless (3 to 5 times), and combine the filtrates. The filtrate was distilled under reduced pressure with a rotary evaporator, and the filtrate was concentrated until viscous. Transfer the concentrate and dry it in a drying oven at 50°C to obtain the crude extract of rosemary. The crude extract is adsorbed by a macroporous adsorption resin, and after being eluted with 40% ethanol, impurities such as chlorophyll can be removed to obtain about 10 kg of fat-soluble rosemary extract.

Embodiment 2: the preparation one of rosemary antibacterial soap

Step 1: Weigh 11 kg of coconut oil, 20 kg of soybean oil, and 30 kg of sunflower oil, put them in a heat-resistant container in turn, and boil them with water-proof heating to keep the temperature of the mixed oil at 35°C-40°C.

Step 2: Weigh 20 kg of water and 9 kg of sodium hydroxide. First put water into a glass container, then slowly add sodium hydroxide, stir rapidly with an alkali-resistant stirrer while adding, completely dissolve sodium hydroxide in water and cool to 35°C to 40°C to obtain an alkali solution.

The third step: Slowly pour the alkali solution in the second step into the glass container containing the mixed oil in the first step, and stir continuously with an alkali-resistant stirrer for about 40 minutes to saponify the two until the two are in solution Mix completely to obtain soap base, and then adjust the pH to 7.5-8.

Step 4: Add 8 kg of rosemary extract to the soap base in the third step while stirring, and then stir evenly to obtain a mixed soap liquid.

Step 5: Brush the inside of the mold with a brush dipped in some olive oil, then pour the soap solution mixed in the fourth step into the mold, cover the mold with a plastic film, and place it in a 35°C incubator for 2 to 3 days. soap.

Step 6: Take out the soap made in step 5 from the mold, put it in a ventilated place and let it dry naturally, about 5 weeks, and get the finished soap.

Embodiment 3: the preparation two of rosemary antibacterial soap

Step 1: Weigh 12 kg of coconut oil, 21 kg of soybean oil, and 25.5 kg of sunflower oil, put them in a heat-resistant container in turn, and boil them with water-proof heating to keep the temperature of the oil at 35°C to 40°C.

Step 2: Weigh 21 kg of water and 9 kg of sodium hydroxide. First put water into a glass container, then slowly add sodium hydroxide, stir rapidly with an alkali-resistant stirrer while adding, completely dissolve sodium hydroxide in water and cool to 35°C to 40°C to obtain an alkali solution.

The third step: Slowly pour the alkali solution in the second step into the glass container containing the mixed oil in the first step, and stir continuously with an alkali-resistant stirrer for about 40 minutes to saponify the two until the two are in solution Mix completely to obtain soap base, and then adjust the pH to 7.5-8.

Step 4: Add 9 kg of rosemary extract to the soap base in the third step while stirring, and then stir evenly to obtain a mixed soap liquid.

Step 5: Brush the inside of the mold with a brush dipped in some olive oil, then pour the soap solution mixed in the fourth step into the mold, cover the mold with a plastic film, and place it in a 35°C incubator for 2 to 3 days. soap.

Step 6: Take out the soap made in step 5 from the mold, put it in a ventilated place and let it dry naturally, about 5 weeks, and get the finished soap.

Example 4: Preparation Three of Rosemary Antibacterial Soap

Step 1: Weigh coconut oil: 10kg, soybean oil 22kg, and sunflower oil 23kg, put them in a heat-resistant container in turn, boil and dissolve with water-proof heating, so that the temperature of the oil is maintained at 35 ° C ~ 40 ° C .

Step 2: Weigh 20 kg of water and 8.8 kg of sodium hydroxide. First put water into a glass container, then slowly add sodium hydroxide, stir rapidly with an alkali-resistant stirrer while adding, completely dissolve sodium hydroxide in water and cool to 35°C to 40°C to obtain an alkali solution.

The third step: Slowly pour the alkali solution in the second step into the glass container containing the mixed oil in the first step, and stir continuously with an alkali-resistant stirrer for about 40 minutes to saponify the two until the two are in solution Mix completely to obtain soap base, and then adjust the pH to 7.5-8.

Step 4: Add 10 kg of rosemary extract to the soap base in the third step while stirring, and then stir evenly to obtain a mixed soap liquid.

Step 5: Brush the inside of the mold with a brush dipped in some olive oil, then pour the soap solution mixed in the fourth step into the mold, cover the mold with a plastic film, and place it in a 35°C incubator for 2 to 3 days. soap.

Step 6: Take out the soap made in step 5 from the mold, put it in a ventilated place and let it dry naturally, about 5 weeks, and get the finished soap.

Example 5: Preparation Four of Rosemary Antibacterial Soap

Step 1: Weigh coconut oil: 12kg, soybean oil 23kg, and sunflower oil 35kg, put them in a heat-resistant container in turn, boil and dissolve with water-proof heating, so that the temperature of the oil is maintained at 35 ℃ ~ 40 ℃ .

Step 2: Weigh 25 kg of water and 9 kg of sodium hydroxide. First put water into a glass container, then slowly add sodium hydroxide, stir rapidly with an alkali-resistant stirrer while adding, completely dissolve sodium hydroxide in water and cool to 35°C to 40°C to obtain an alkali solution.

The third step: Slowly pour the alkali solution in the second step into the glass container containing the mixed oil in the first step, and stir continuously with an alkali-resistant stirrer for about 40 minutes to saponify the two until the two are in solution Mix completely to obtain soap base, and then adjust the pH to 7.5-8.

Step 4: Add 10 kg of rosemary extract to the soap base in the third step while stirring, and then stir evenly to obtain a mixed soap liquid.

Step 5: Brush the inside of the mold with a brush dipped in some olive oil, then pour the soap solution mixed in the fourth step into the mold, cover the mold with a plastic film, and place it in a 35°C incubator for 2 to 3 days. soap.

Step 6: Take out the soap made in step 5 from the mold, put it in a ventilated place and let it dry naturally, about 5 weeks, and get the finished soap.

Embodiment 6: the preparation five of rosemary antibacterial soap

Step 1: Weigh 10 kg of coconut oil, 20 kg of soybean oil, and 20 kg of sunflower oil, put them in a heat-resistant container in turn, and boil them with water-proof heating to keep the temperature of the mixed oil at 35°C to 40°C.

Step 2: Weigh 20 kg of water and 8.5 kg of sodium hydroxide. First put water into a glass container, then slowly add sodium hydroxide, stir rapidly with an alkali-resistant stirrer while adding, completely dissolve sodium hydroxide in water and cool to 35°C to 40°C to obtain an alkali solution.

The third step: Slowly pour the alkali solution in the second step into the glass container containing the mixed oil in the first step, and stir continuously with an alkali-resistant stirrer for about 40 minutes to saponify the two until the two are in solution Mix completely to obtain soap base, and then adjust the pH to 7.5-8.

Step 4: Add 5 kg of rosemary extract to the soap base in the third step while stirring, and then stir evenly to obtain a mixed soap liquid.

Step 5: Brush the inside of the mold with a brush dipped in some olive oil, then pour the soap solution mixed in the fourth step into the mold, cover the mold with a plastic film, and place it in a 35°C incubator for 2 to 3 days. soap.

Step 6: Take out the soap made in step 5 from the mold, put it in a ventilated place and let it dry naturally, about 5 weeks, and get the finished soap.

Embodiment 7:

Carry out antibacterial experiment with the rosemary scented soap of embodiment 4:

(1) Preparation of antibacterial solution: The prepared rosemary soap and conventional soap (non-antibacterial soap) were prepared into a 1:10 solution respectively.

(2) Use 102-type medium-speed qualitative filter paper to make a circular filter paper piece with a diameter of 4mm, place it in a petri dish for sterilization at 121°C for 20 minutes, and after cooling, add 20 μl of the solution prepared in (1) to the filter paper piece quantitatively and dropwise. , dried at 25°C for later use.

(3) The tested strains are: Clostridium perfringens, Staphylococcus aureus, Bacillus anthracis, Salmonella, Mycobacterium tuberculosis, Streptococcus A, Streptococcus pneumoniae, Shigella dysenteriae, and Shigella sonneri. The activated test bacteria were inoculated in MH sausage medium, cultured at 37°C for 24 hours, and diluted with MH medium to obtain a bacterial suspension with a concentration of 10 ⁵ -10 ⁸ CFU/ml.

(4) Add 15ml of MHA medium to a 9cm-diameter petri dish, add 1ml of bacterial suspension with a concentration of 10 ⁵ ~ 10 ⁸ CFU/ml to each dish, spread it evenly on the MHA medium with a spreader, and then add the added The filter paper sheets with the antibacterial solution and the filter paper sheets of the control solution were evenly pasted on the surface of the plate in turn, and 5 parallel tests were done for each bacterium. After culturing at 37°C for 24 hours, observe and record the size of the bacteriostatic zone (transparent zone), and take the average value of the bacteriostatic zone size. The antibacterial results are shown in Table 1.

Table 1: bacteriostatic agent solution to the inhibition test result of several test bacteria

Note: The measured results are mean ± standard error

The results show that rosemary soap has a certain degree of antibacterial effect on the pathogenic bacteria involved in the test, especially the antibacterial effect on Bacillus anthracis, Salmonella, Shigella sonnii and Escherichia coli. Compared with conventional soap, the antibacterial effect of rosemary soap is significantly improved.

The basic principles and main features of the present invention and the advantages of the present invention have been shown and described above. Those skilled in the industry should understand that the present invention is not limited by the above-mentioned embodiments. What are described in the above-mentioned embodiments and the description only illustrate the principle of the present invention. Without departing from the spirit and scope of the present invention, the present invention will also have Variations and improvements are possible, which fall within the scope of the claimed invention. The protection scope of the present invention is defined by the appended claims and their equivalents.

Claims (6)

1. a kind of rosemary natural extract is characterized in that, makes according to the following steps: take by weighing 50 kilograms of rosemary dry sample, rosemary dry sample is ground into powder, soak 24h with 20 times of amount of 80% ethanol, Then use a Buchner funnel to filter and collect the filtrate; repeat this operation until the ethanol soaking solution is colorless, and combine the filtrate; use a rotary evaporator to distill the filtrate under reduced pressure, and concentrate the filtrate to obtain a crude extract of rosemary; the crude extract is absorbed by macropores Resin adsorption, after removing impurities such as chlorophyll, about 10 kilograms of fat-soluble rosemary extracts were obtained. 2. a kind of antibacterial soap, it is characterized in that, antibacterial activity and aromatic component in the described antibacterial soap are rosemary natural extract as claimed in claim 1. 3. A kind of antibacterial soap according to claim 2, is characterized in that, comprises 5-10 parts of rosemary extracts, 10-12 parts of coconut oils, 20-23 parts of soybean oils, sunflower seed oils according to mass parts 20-35 parts, 8.5-9 parts of sodium hydroxide, 20-25 parts of water. 4. a kind of antibacterial scented soap according to claim 2 is characterized in that, according to mass parts by 5-10 parts of rosemary extract, 10-12 parts of coconut oil, 20-23 parts of soybean oil, sunflower seed oil 20-35 parts, 8.5-9 parts of sodium hydroxide, 20-25 parts of water. 5. a preparation method of antibacterial soap as claimed in claim 4, is characterized in that, its step comprises: the first step: coconut oil, soybean oil, sunflower seed oil are put into heat-resistant container in proportion by weight , use water-proof heating method to boil and dissolve, so that the temperature of the oil is maintained at 35 ° C ~ 40 ° C; the second step: according to the required amount of water and sodium hydroxide, put the water into a glass container, and then slowly add hydroxide Sodium, stir quickly with an alkali-resistant stirrer while adding, the sodium hydroxide is completely dissolved in water and cooled to 35°C-40°C to obtain an alkali solution; the third step: slowly pour the alkali solution in the second step into the first In the glass container with mixed oil in the step, while adding it, stir continuously with an alkali-resistant stirrer for about 40 minutes to saponify the two until the two solutions are completely mixed to obtain a soap base, and adjust the pH to 7.5-8; Step 4: Add rosemary extract to the soap base in the third step in proportion while stirring, and then stir evenly to obtain a mixed soap liquid; Step 5: Use a brush dipped in a small amount of olive oil to paint the inside of the mold, and then put The fourth step is to pour the mixed soap liquid into the mold, cover the mold with plastic film, and place it in a 35°C incubator for 2 to 3 days to obtain soap; the sixth step: take out the soap prepared in the fifth step from the mold, Put it in a ventilated place and let it dry naturally to obtain a finished soap. 6. a kind of bacteriostatic soap as claimed in claim 2,3 or 4, is characterized in that, described bacteriostatic soap is in the inhibition Clostridium perfringens, Staphylococcus aureus, Bacillus anthracis, Salmonella, tuberculosis divergence Bacillus, Streptococcus A, Streptococcus pneumoniae, Shigella Shigella or Shigella Sonnei.