CN104807792A - Negative refractive index photonic crystal bioluminescence detector - Google Patents

Abstract

The invention discloses a negative refractive index photonic crystal type biological fluorescence detector, which comprises a main body box, an optical base and a transparent colloid, and the negative refractive index photonic crystal in the main body box is alternately overlapped by piezoelectric material layers and negative refractive index material layers Forming, the piezoelectric material layer is electrically connected to the control power supply, and the narrow-band transmission characteristics of the negative refractive index photonic crystal are changed. The negative refractive index photonic crystal is attached to the optical substrate, and the first photodetector and the negative refractive index photonic crystal are arranged on the upper surface of the negative refractive index photonic crystal. The second photodetector, the first photodetector and the second photodetector receive the excited fluorescent signal filtered by the negative refractive index photonic crystal, and convert the excited fluorescent signal into an electrical signal, and the electrical signal is sent to the processor for processing . The invention can realize the selection of the excitation laser wavelength and the filtering of the fluorescence wavelength with a single device, and can detect multiple fluorescent tissues at the same time, which solves the quantitative calculation problem caused by different wavelengths, and the fluorescence measurement accuracy reaches 96.8%.

Description

A Negative Refractive Index Photonic Crystal Type Bioluminescence Detector

technical field

The invention relates to the technical field of biological tissue autofluorescence spectrum analysis, in particular to a negative refractive index photonic crystal bioluminescence detector.

Background technique

The fluorescence emission spectrum is the result of the fluorescent substance absorbing radiation and then emitting it. When the fluorescence excitation spectrum refers to the relative efficiency of emitting fluorescence of a certain wavelength caused by the excitation of different wavelengths, the fluorescence intensity is expressed by the integral area of the fluorescence emission spectrum of the fluorescent substance The amount of fluorescence intensity. In recent years, with the emergence of different fluorescent markers, it has become possible to label different organelles in cells or detect different ions in cells. The appropriate excitation light source is selected according to the fluorescence emission characteristics of fluorescent markers. In vivo fluorescence imaging technology has just developed in the past few years. Within a short period of time, it has been widely used in cell tracking, tumor diagnosis and drug development, and has shown great potential in clinical application.

At present, the fluorescence analysis method in biological tissue is favored by analysts due to its high sensitivity, good selectivity, and easy operation. The application of fluorescence analysis method to drug analysis has been used in the analysis and identification of drug active ingredients, pharmacokinetic research, Clinical drug and pharmacodynamic analysis have made great progress, and are widely used in trace analysis in biochemical analysis, biomedicine and other fields.

A number of patents have been published in my country, such as "A Microfluidic System and Method for Detection and Screening of Single-beam Biological Cells" (CN103439242 A), which uses up-conversion luminescent nanomaterials to mark problematic cells in the target to be tested, and uses near-infrared band The method of laser-induced up-conversion luminescence detects problematic cells through laser scanning optical detection means. "A method for rapidly detecting glucose concentration based on gold nanocluster fluorescent probe" (CN103837516 A) and "device and method for detecting fluorophore-labeled biological components" (CN101410708A) then provide a simple A method for labeling biological components with fluorophores in liquid samples for technical analysis. In order to obtain the corresponding fluorescence characteristics, the above-mentioned invention patents all use an excitation light source with a single incident wavelength. This excitation light source cannot be adjusted, has a single function, and cannot identify multiple fluorescent tissues at the same time.

Contents of the invention

In view of the above prior art, the object of the present invention is to provide a negative refractive index photonic crystal bioluminescent detector, which aims to realize the selection of the excitation laser wavelength and the adjustment of the fluorescence excitation wavelength of biological tissue by using a single device, so as to achieve multiple The purpose of detecting two kinds of fluorescent tissues at the same time, and effectively avoid the interference of fluorescent tissues of other wavelengths.

In order to solve the above technical problems, the present invention adopts the following technical solutions:

A negative refractive index photonic crystal bioluminescent detector, comprising an optical base 12 and a transparent colloid 13, the optical base is located on the transparent colloid, and is characterized in that it also includes a main body box 11, and the main body box 11 is placed on the optical base 12 Above, the main body box is mainly composed of laser 14, collimator lens 15, display module 16, laser drive circuit 18, processor, negative refractive index photonic crystal 110, first photodetector 111, second photodetector 113 and control power supply 115 constitutes; the processor is connected to control the laser drive circuit 18, the laser drive circuit 18 drives the laser, and the collimator lens 15 is installed directly below the laser 14; the negative refractive index photonic crystal 110 is composed of a piezoelectric material layer and a negative refractive index material The layers are alternately overlapped and formed, the piezoelectric material layer is electrically connected to the control power supply, and the narrow band of the negative refractive index photonic crystal is changed. The negative refractive index photonic crystal 110 is attached to the optical substrate, and the upper surface of the negative refractive index photonic crystal 110 is provided with the The first photodetector 111 and the second photodetector 113, the first photodetector 111 and the second photodetector 113 are located on both sides below the collimator lens 15; the first photodetector 111 and the second photodetector 113 receive The excited fluorescence signal filtered by the negative refractive index photonic crystal 110 is converted into an electrical signal, and the electrical signal is sent to the processor for processing, and the processing result is displayed on the display module 16 .

In the present invention, the negative refractive index photonic crystal bioluminescence detector further includes a transparent colloid on which the optical base is arranged, and the main body box 11 is located directly above the optical base.

In the present invention, the processor is a DSP processor, an ARM processor or a single-chip microcomputer.

In the present invention, the main body box 11 further includes a light-absorbing coating 112 , which is attached to the optical substrate 12 to absorb excess scattered fluorescence transmitted from the skin and reduce environmental interference signals.

In the present invention, a fluorescence wavelength adjustment button 17 is also arranged on the main box, and the fluorescence wavelength adjustment button is connected with the P processor 19 to control the laser to emit different specific excitation wavelengths.

In the present invention, the laser 14 is a short-pulse adjustable wavelength laser with a wavelength range of 150nm to 460nm.

Further, the short-pulse tunable wavelength laser is an ultraviolet or near-violet pulsed laser with a wavelength of 230nm-345nm.

In the present invention, the negative refractive index photonic crystal 110 is composed of a one-dimensional negative refractive index photonic crystal, and has both narrow-band and broadband filtering characteristics.

In the present invention, the transparent colloid is made of soft polyvinyl chloride sol, has isotropic characteristics, and has a thickness of less than 0.2mm.

In the present invention, the first photodetector 111 and the second photodetector 113 are photodiodes or photomultiplier tubes with a detection range of 860-1500 nm, and an optimal detection range of 930-1150 nm.

Working principle of the present invention is:

After the excitation light enters the collimating lens, it enters the negative refractive index photonic crystal. Since the negative refractive index photonic crystal is composed of negative refractive index material and piezoelectric material, and has both narrow-band and broadband filtering characteristics, the piezoelectric material is affected by Control the power supply so that the narrow band of the negative refractive index photonic crystal moves; if the wavelength of the excitation light is within the narrow range of the negative refractive index photonic crystal, the excitation light enters the skin after passing through the negative refractive index photonic crystal, the optical substrate and the transparent colloid, Thereby forming fluorescence with the corresponding biological tissue, the excited fluorescence is transmitted from the skin, passes through the optical substrate and the negative refractive index photonic crystal in turn, and is received by the photodetector to become an electrical signal, which is sent to the processor for further processing. Processing; when the wavelength of the excitation light does not fall within the narrow band of the negative refractive index photonic crystal, the excitation light is totally reflected by the negative refractive index photonic crystal and cannot enter the skin, thereby avoiding signal interference of other excitation wavelengths.

Compared with the prior art, the present invention has the following beneficial effects:

1. The incident wavelength of the excitation light source is controllable. Adjust the emission wavelength of the laser through the laser drive circuit, and at the same time control the power supply to adjust the refractive index of the piezoelectric material, thereby directly affecting the propagation characteristics of the negative refractive index photonic crystal, to adjust the narrow-band filter characteristics of the excitation light, to match the fluorescence spectrum, and to solve different problems. Quantitative calculation problems caused by wavelength, improve fluorescence measurement accuracy to 96.8%.

2. The selection of the excitation laser wavelength and the filtering of the fluorescence wavelength can be realized by using a single device. The laser can be controlled to emit different specific excitation wavelengths through the fluorescence wavelength adjustment button and the narrow-band filtering characteristics of the negative refractive index photonic crystal can be used to realize a variety of fluorescent tissues. Simultaneous detection, easy to use and convenient to implement.

3. A light-absorbing coating is provided on the optical substrate, and the excitation light is selected for narrow-band filtering. The light-absorbing coating absorbs excess scattered fluorescence transmitted from the skin, which can effectively avoid the influence of other fluorescent tissues.

Description of drawings

Fig. 1 is a structural schematic diagram of Embodiment 1 of the present invention;

2 is a schematic diagram of a periodic structure of a negative refractive index photonic crystal in Embodiment 1 of the present invention;

Fig. 3 is a transmission characteristic diagram of a negative refractive index photonic crystal in Embodiment 1 of the present invention;

FIG. 4 is a characteristic diagram of transmission of a negative refractive index photonic crystal under different modulations in Embodiment 1 of the present invention.

FIG. 5 is a graph of fluorescence energy excited by laser light with different wavelengths in Example 1 of the present invention.

Reference numerals are: 11 is the main body box, 12 is the optical substrate, 13 is the transparent colloid, 14 is the laser, 15 is the collimating lens, 16 is the display module, 17 is the fluorescent wavelength adjustment button, 18 is the laser driving circuit, 19 is DSP processor, 110 is a negative refractive index photonic crystal, 111 is a first photodetector, 112 is a light-absorbing coating, 113 is a second photodetector, 114 is a built-in power supply, and 115 is a control power supply.

Detailed ways

The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

A negative refractive index photonic crystal bioluminescent detector, comprising an optical base 12 and a transparent colloid 13, the optical base is located on the transparent colloid, and is characterized in that it also includes a main body box 11, and the main body box 11 is placed on the optical base 12 Above, the main body box is mainly composed of laser 14, collimating lens 15, display module 16, laser drive circuit 18, DSP processor 19, negative refractive index photonic crystal 110, first photodetector 111, second photodetector 113 and Control power supply 115 forms; DSP processor 19 connects and controls laser drive circuit 18, and laser drive circuit 18 drives laser 14, and described collimating lens 15 is installed directly below laser 14; Described negative refractive index photonic crystal is made of piezoelectric material layer and Negative refractive index material layers are alternately overlapped and formed, and the piezoelectric material layer is electrically connected to the control power source to change the narrow band of the negative refractive index photonic crystal. The negative refractive index photonic crystal 110 is attached to the optical substrate, and the negative refractive index photonic crystal 110 is The first photodetector 111 and the second photodetector 113 are arranged on the surface, and the first photodetector 111 and the second photodetector 113 are located on both sides below the collimating lens 15; The detector 113 receives the excited fluorescent signal filtered by the negative refractive index photonic crystal 110 , and converts the excited fluorescent signal into an electrical signal, which is sent to the DSP processor 19 for processing, and the processing result is displayed on the display module 16 . The present invention adopts a built-in power supply 114 and has good portability.

The formation mechanism of the present invention: the negative refractive index photonic crystal 110 is made of negative refractive index material and piezoelectric material, and has narrow-band and broadband filtering characteristics at the same time, wherein the piezoelectric material is controlled by the control power supply 115, so that the negative refractive index photonic crystal The narrow band of 110 moves; if the wavelength of the excitation light is within the range of the narrow band of the negative refractive index photonic crystal 110, the excitation light enters the skin after passing through the negative refractive index photonic crystal 110, the optical substrate 12 and the transparent colloid 13, thereby matching with the corresponding biological The tissue forms fluorescence, and the excited fluorescence is transmitted from the skin, passes through the optical substrate 12 and the negative refractive index photonic crystal 110 in turn, and is received by the first photodetector and the second photodetector 113, and the received signal is sent to the DSP processor 19 In this case, the excess scattered fluorescence is absorbed by the light-absorbing coating material 112 to reduce environmental interference signals.

Embodiment one:

The structure of a negative refractive index photonic crystal bioluminescence detector in this example is shown in FIG. 1 . The negative refractive index photonic crystal is composed of negative refractive index materials with a refractive index of -0.65 and piezoelectric materials with a refractive index of 2.297 alternately, wherein the jth layer of piezoelectric material is controlled by a control power supply 115, as shown in Figure 2 .

The transmission characteristic diagram of the negative refractive index photonic crystal is shown in Figure 3, which has both narrow-band and broadband filtering characteristics, in which there is a very sharp filtering bandwidth in the short-wavelength region (wavelength range of 324nm~326nm) and long-wavelength region (wavelength The range is 864~1088nm) has a wide filter bandwidth, light can only pass through the negative refractive index photonic crystal 110 at these two wavelengths.

The thickness of the piezoelectric material made of dielectric elastic material can be changed by controlling the applied voltage of the power supply 115, and the empirical formula can be expressed as:

                                         (1)

                                        (2)

Among them, the elastic strain amplitude is related to the applied voltage, is the thickness of the piezoelectric material, is the elastic-optic coefficient of the piezoelectric material, and is the modulation depth. When the modulation depth is , , and the changes of the narrow-band and broadband filtering characteristics of the negative refractive index photonic crystal in three cases, as shown in Figure 4.

At the same time, the fluorescence excited by different short-wavelength lasers is related to the excitation wavelength. In this way, as long as the excitation light is greater than a certain value, it can be excited, but there is a quantitative problem. The energy of photoluminescence obtained by different excitation wavelengths is different, as shown in Figure 5. Show.

When measuring, different specific excitation wavelengths can be selected by the fluorescent wavelength adjustment button 17. After the DSP processor 19 receives the selected wavelength, the emission wavelength of the laser 14 is controlled by the laser drive circuit 18, and the processor 19 sends the preset wavelength simultaneously. The voltage signal is sent to the control power supply 115, and the control power supply 115 controls the piezoelectric material through the voltage, so that the narrow band of the negative refractive index photonic crystal 110 moves, so that the narrow band filtering characteristics can just meet the wavelength of the laser 14, and realize narrow band filtering, then the excitation light passes through the negative The refractive index photonic crystal 110, the optical substrate 12, and the transparent colloid 13 enter the skin, thereby forming fluorescence with the corresponding biological tissue, and the excited fluorescence is transmitted from the skin, passing through the optical substrate 12 and the negative refractive index photonic crystal 110 in turn, and then being absorbed by the first photonic crystal. One photodetector and the second photodetector receive, and the received signal is sent to the DSP processor 19 for post-processing. Since the two detectors can be used for correlation operations, the processing efficiency and accuracy are effectively improved, and the processed result is finally processed. sent to the display module 16. In this way, the trouble of changing the laser light source can be avoided, and the quantitative detection can be carried out. At the same time, the interference from other fluorescence can be avoided, and the measurement accuracy of fluorescence can be improved to 96.8%.

Since the current calculation method of the photonic crystal energy band structure is mature, it is enough to ensure that the required band gap range in the structure is formed through a reasonable combination of materials. forbidden band characteristics.

It should be understood that the above-mentioned embodiments are only used to illustrate the technical solution of the present invention rather than limit it. Those skilled in the art can easily construct a negative refractive index photonic crystal bioluminescence detector different from the first embodiment by designing different photonic crystal band gap features. Accordingly, the present invention is intended to embrace all those alterations, modifications and variations that come within the scope of the appended claims.

Claims (10)

1. a negative index photonic crystal type bioluminescence detector, it is characterized in that, comprise optical substrate (12), main body box (11), described main body box (11) is placed on optical substrate (12), and main body box is primarily of laser instrument (14), collimation lens (15), display module (16), drive circuit for laser (18), processor (19), negative index photonic crystal (110), the first photodetector (111), the second photodetector (113) and control power supply (115) formation; Processor (19) connection control drive circuit for laser (18), drive circuit for laser (18) drive laser, installs described collimation lens (15) immediately below laser instrument (14); Described negative index photonic crystal (110) is by piezoelectric material layer and material with negative refractive index layer is mutual is alternately stackedly formed, piezoelectric material layer is connected with control power electric, change negative index photonic crystal arrowband, described negative index photonic crystal (110) is attached in optical substrate (12), the upper surface of negative index photonic crystal (110) arranges described first photodetector (111) and the second photodetector (113), and the first photodetector (111) and the second photodetector (113) are positioned at both sides, collimation lens (15) below; First photodetector (111) and the second photodetector (113) receive through the filtered fluorescence excitation signal of negative index photonic crystal (110), and convert this fluorescence excitation signal to electric signal, electric signal sends processor to and processes, and result is presented on display module (16).

2. negative index photonic crystal type bioluminescence detector according to claim 1, is characterized in that, also comprise transparent colloid (13), and transparent colloid is arranged described optical substrate (12), described main body box (11) is positioned at directly over optical substrate.

3. negative index photonic crystal type bioluminescence detector according to claim 1, is characterized in that, described processor is dsp processor or arm processor or single-chip microcomputer.

4. negative index photonic crystal type bioluminescence detector according to claim 1, it is characterized in that, described main body box (11) also comprises light-absorbing coating (112), and described light-absorbing coating is attached in optical substrate (12).

5. negative index photonic crystal type bioluminescence detector according to claim 1 and 2, is characterized in that, described main body box is also provided with wavelength of fluorescence adjustment button (17), and described wavelength of fluorescence adjustment button is connected with processor.

6. negative index photonic crystal type bioluminescence detector according to claim 1, is characterized in that, described laser instrument (14) is short pulse tunable laser, and wavelength coverage is 150nm ~ 460nm.

7. negative index photonic crystal type bioluminescence detector according to claim 4, is characterized in that, described short pulse tunable laser is wavelength is the ultraviolet of 230nm ~ 345nm or nearly purple light pulsed laser.

8. negative index photonic crystal type bioluminescence detector according to claim 1, is characterized in that, described negative index photonic crystal (110) is made up of one dimension negative index photonic crystal.

9. negative index photonic crystal type bioluminescence detector according to claim 1, is characterized in that, described transparent colloid (13) is made up of flexible PVC colloidal sol, and thickness is less than 0.2mm.

10. negative index photonic crystal type bioluminescence detector according to claim 1, it is characterized in that, described first photodetector (111) and the second photodetector (113) are photodiode or photomultiplier, and sensing range is 860 ~ 1500nm.