CN104784505A - Dendrobium officinale healthcare product with auxiliary blood glucose reducing function and preparation method thereof - Google Patents
Dendrobium officinale healthcare product with auxiliary blood glucose reducing function and preparation method thereof Download PDFInfo
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- CN104784505A CN104784505A CN201510128319.7A CN201510128319A CN104784505A CN 104784505 A CN104784505 A CN 104784505A CN 201510128319 A CN201510128319 A CN 201510128319A CN 104784505 A CN104784505 A CN 104784505A
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- schisandrae chinensis
- fructus schisandrae
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- radix puerariae
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Abstract
The invention discloses a dendrobium officinale healthcare product with an auxiliary blood glucose reducing function. The dendrobium officinale healthcare product comprises the following raw materials in parts by weight: 35 to 45 parts of fresh dendrobium officinale bars, 1 to 2 parts of American ginseng, 2 to 4 parts of the root of kudzu vine, 2 to 4 parts of radix trichosanthis and 1 to 2 parts of fructus schizandrae. A preparation method for the dendrobium officinale healthcare product with the auxiliary blood glucose reducing function comprises the following steps: weighing the raw materials; processing the fresh dendrobium officinale bars; processing the American ginseng; performing alcohol extracting; performing water extracting; drying; batching. According to the dendrobium officinale healthcare product with the auxiliary blood glucose reducing function disclosed by the invention, the raw material formula is from clinical practice under the guidance of the theory of traditional Chinese medicine; the herbal medicines in a prescription are concise and are high in pertinency; the medicines in a raw material formula can nourish yin, benefit qi, clear heat and promote the secretion of saliva or body fluid; the active ingredients of the medicines are easy to absorb and are favorable to play efficacy; the medicinal materials in the raw material formula have the homology of medicine and food, and have no toxic or side effect.
Description
Technical field
The present invention relates to Dendrobium officinale health product of a kind of auxiliary hyperglycemic and preparation method thereof, belong to field of health care food.
Background technology
The clinical syndrome of diabetes (diabetes mellitus, DM) to be jointly caused by h and E factor one group with carbohydrate metabolism disturbance be clinical manifestation.It is by insulin deficit insulin action obstacle thus cause the metabolism disorder of three large material and water and eletrolytes etc. alone or in combination; Be principal character clinically with chronic hyperglycemia, with polyphagia, polydipsia, polyuria, lose weight etc. as main clinical manifestation.
At present, diabetes have worldwide become the 3rd large chronic disease of serious harm human health after cardiovascular and cerebrovascular disease, tumor.According to Nature magazine recent statistics in 2012, China type 2 diabetes mellitus patient is about 9,000 ten thousand people, and the number dying from diabetic complication every year has 1,300,000, occupy first place, the world.Diabetes are one of world-wide public health hygienic issues, clinically diabetes are mainly divided into amphitypy.Type Ⅰ diabetes mellitus (insulin-dependent) causes hyperglycemia because B cell infringement causes level of insulin secretion extremely low, account for diabetes patient's 10%, at present except giving exogenous insulin and insulin replacement therapy, there is no effective way.Type Ⅱdiabetes mellitus (non-insulin-depending type) is that the unsound and hyperamization sugar level of insulin secretion relative deficiency and insulin action link raises.Along with to the pathogenetic deeply understanding of type Ⅱdiabetes mellitus, developed there is number of mechanisms antidiabetic thing for clinical.Mainly contain insulin secretion stimulators, euglycemic agent, glycosidase inhibitor.The medicine for the treatment of chronic complicating diseases of diabetes mainly contains aldose reductase inhibitor, albumen nonenzymatic glycosylation inhibitor etc.Chemicals has good hypoglycemic activity, but to improving clinical symptoms poor effect, and invalid to diabetic complication; In addition, chemicals majority has certain side effect.
The traditional Chinese medical science claims diabetes to be diabetes, treatment by Chinese herbs diabetes has a long history, there is unique Theories and methods, as far back as B.C. 2nd century, Huangdi's Internal Classics just existing discussion points out that " this person must count the sweet and refreshing and many fertilizer of food also, overeating greasy food bringing about internal heat, over-eating the food with sweet flavor bringing about abdominal distension; its gas overflow of event, transfers to and quenching one's thirst.The traditional Chinese medical science think diabetes be due to congenital examine compose not enough, multiple because of the reason such as disorder of emotion, eating and drinking without temperance cause scorching for basic pathogenesis with the deficiency of YIN, with polyuria, polydipsia, polyphagia, weak, become thin, or urinate pleasantly sweet a kind of disease for typical clinical manifestations.Based on clearing heat and moistening dryness, YIN nourishing and the production of body fluid promoting, there is potential value to treating and preventing diabetes in treatment, therefore, in prevention and corntrol chronic complicating diseases of diabetes, raising quality of life of patients, has great advantage.
Herba Dendrobii (Tiepishihu) DENDROBII OFFICINALIS CAULIS, this product is the dry stem of orchid Herba Dendrobii DendrobiumofficinaieKimuraetMigo.There is reinforcing stomach reg fluid, the effect of nourishing YIN and clearing away heat.For calentura Tianjin wound, xerostomia excessive thirst, deficiency of stomach-Yin, lack of appetite is in vomitting, and deficiency-heat is not moved back after being ill, hyperactivity of fire caused by deficiency of YIN, hectic fever due to YIN-deficiency consumptive fever, poor vision, and muscles and bones flaccidity is soft.
Radix Panacis Quinquefolii (Xiyangshen) PANACIS QUINQUEFOLII RADIX this product is the dry root of Araliaceae Radix Panacis Quinquefolii .PanaxquinquefoliumL..Be all cultivation product, autumn excavates, and cleans, dries or cold drying.There is boosting qi and nourishing yin, the effect of clearing away heat and promoting production of body fluid.Lose for deficiency of vital energy the moon, deficiency-heat is tired of tired, and cough with asthma expectorant blood, interior-heat is quenched one's thirst, dryness of the mouth and throat.
Radix Puerariae (Gengen) PUERARIAE LOBATAE RADIX this product is the dry root of legume pueraria lobata Puerarialobata (Willd.) Ohwi.Practise and claim Herba Gelsemii Elegantis.Autumn, season in winter two excavate, and take advantage of fresh-cut and become sheet or fritter; Dry.There is expelling pathogenic factors from muscles for reducing heat, promoting the production of body fluid to quench thirst, rash, yang invigorating antidiarrheal, dredge the meridian passage, deinebriating effect.For fever caused by exogenous pathogens headache, stiff nape and back, thirsty, quench one's thirst, measles without adequate eruption, hematodiarrhoea, has loose bowels, dizziness and headache, apoplectic hemiplegia, obstruction of qi in the chest and cardialgia, damage of the spleen and stomach caused by alcoholism.
The dry root of Radix Trichosanthis (Tianhuafen) TRICHOSANTHIS RADIX this product TrichosantheskirilowiiMaxim. or trichosanthes rosthornii Harms TrichosanthesrosthorniiHarIlls that has been cucurbitaceous plant Fructus Trichosanthis.Autumn, season in winter two excavate, and clean, removing crust, and cutting or vertical profile become lobe, dry.There is clearing away heat-fire, promoting the production of body fluid to quench thirst, the effect of detumescence and apocenosis.For calentura excessive thirst, lung-heat type cough, interior-heat is quenched one's thirst, sore swollen toxin.
Fructus Schisandrae Chinensis (Wuweizi) SCHISANDRAE CHINENSIS FRUCTUS this product is the dry mature fruit of magnoliaceae schisandra schisandrnfhinensis (Turcz.) Baill., practises and claims " Radix Schisandrae Bicoloris ".Pluck during fruit maturation in autumn, dry or dry after steaming, removing carpopodium and impurity.There is convergence astringent or styptic treatment for spontaneous sweating, supplementing QI for promoting the production of body fluid, the effect of kidney calming.For chronic cough dyspnea due to deficiency, emission, enuresis frequent micturition, incessant chronic diarrhea, spontaneous sweating, Tianjin wound is thirsty, and interior-heat is quenched one's thirst, palpitation and insomnia.
Summary of the invention
The object of this invention is to provide Dendrobium officinale health product of a kind of auxiliary hyperglycemic and preparation method thereof, full side's treating both the principal and secondary aspects of a disease, play Yin-nourishing and body fluid promoting altogether except heat effect, have good prophylactic-therapeutic effect to diabetes.
To achieve these goals, present invention employs following technical scheme:
A Dendrobium officinale health product for auxiliary hyperglycemic, counts by weight, and raw material components is as follows: the fresh bar of Herba Dendrobii 35 ~ 45 parts, Radix Panacis Quinquefolii 1 ~ 2 part, Radix Puerariae 2 ~ 4 parts, Radix Trichosanthis 2 ~ 4 parts and Fructus Schisandrae Chinensis 1 ~ 2 part.
The Dendrobium officinale health product of auxiliary hyperglycemic as claimed in claim 1, is characterized in that, counts by weight, and raw material components is as follows: Herba Dendrobii 40 parts, Radix Panacis Quinquefolii 1 part, Radix Puerariae 3 parts, Radix Trichosanthis 3 parts and Fructus Schisandrae Chinensis 1.5 parts.
A preparation method for the Dendrobium officinale health product of auxiliary hyperglycemic, comprises the steps:
(1) raw material takes: count by weight, takes following raw material components respectively, the fresh bar of Herba Dendrobii 35 ~ 45 parts, Radix Panacis Quinquefolii 1 ~ 2 part, Radix Puerariae 2 ~ 4 parts, Radix Trichosanthis 2 ~ 4 parts and Fructus Schisandrae Chinensis 1 ~ 2 part;
(2) process of the fresh bar of Herba Dendrobii: the fresh bar of Herba Dendrobii step (1) taken is cleaned, drain after, drop in Chinese medicine grinder and be ground into the long Herba Dendrobii segment of 1-2cm; Herba Dendrobii segment is carried out vacuum lyophilization, then carries out micronizing, obtain Herba Dendrobii fine powder;
(3) process of Radix Panacis Quinquefolii: the Radix Panacis Quinquefolii that step (1) takes is placed in the hot air circulation drying oven that temperature is less than 60 DEG C and carries out drying, then carry out micronizing, obtain Radix Panacis Quinquefolii fine powder;
(4) alcohol extraction:
Get Radix Puerariae and Fructus Schisandrae Chinensis that step (1) takes, add 75 ~ 80% ethanol of Radix Puerariae and Fructus Schisandrae Chinensis gross weight 8 ~ 10 times, stirring and refluxing is extracted 2 ~ 3 times, each 1.3 ~ 1.8 hours, backflow left standstill more than 12 hours, Aspirate supernatant, obtain Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear liquid, merge supernatant, become Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear paste for subsequent use Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear liquid concentrating under reduced pressure, Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction medicinal residues are separately preserved;
(5) water extraction:
Get the Radix Trichosanthis that step (1) takes, add the Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction medicinal residues that step (4) obtains, add the water of Radix Trichosanthis and Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction medicinal residues gross weight 8 ~ 10 times again, stirring and refluxing is extracted 2 ~ 3 times, each 1.8 ~ 2.2 hours, backflow left standstill more than 12 hours, Aspirate supernatant, obtain Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction clear liquid, Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction clear liquid concentrating under reduced pressure is become Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction clear paste;
(6) dry:
Be dried to dry cream Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear paste is less than the environment of 60 DEG C in temperature under, then be ground into Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction fine powder;
Be dried to dry cream Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction clear paste is less than the environment of 60 DEG C in temperature under, then be ground into Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction fine powder;
(7) prepare burden:
Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction fine powder 1 ~ 2 part of mix homogeneously that the Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction fine powder 1 ~ 2 part that the Radix Panacis Quinquefolii fine powder 1 ~ 2 part that the Herba Dendrobii fine powder 1 ~ 2 part step (2) obtained, step (3) obtain, step (6) obtain and step (6) obtain, obtains the Dendrobium officinale health product of described auxiliary hyperglycemic.
The relative density of Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear paste 60 DEG C time is 1.15 ~ 1.25.
The relative density of Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction clear paste 60 DEG C time is 1.15 ~ 1.25.
The process conditions of alcohol extraction are: add Radix Puerariae and Fructus Schisandrae Chinensis gross weight 10 times amount 80% ethanol, and stirring and refluxing extracts twice, each 1.5 hours.
The process conditions of water extraction are: the water adding Radix Trichosanthis and Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction medicinal residues gross weight 10 times amount, stirring and refluxing extracts 3 times, each 2 hours.
The process conditions of batching are: Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction fine powder 1 part of mix homogeneously that the Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction fine powder 1 part that the Radix Panacis Quinquefolii fine powder 1 part that the Herba Dendrobii fine powder 1 part step (2) obtained, step (3) obtain, step (6) obtain and step (6) obtain.
The Dendrobium officinale health product of described auxiliary hyperglycemic conveniently technology of preparing is prepared into tablet, granule, hard capsule or soft capsule.
Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction fine powder and Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction fine powder were the fine powder that 80 ~ 140 mesh sieves obtain.
Described Chinese medicine composition is preparing the application in blood sugar lowering class medicine.
75% ethanol described in the present invention herein refers to that volume fraction is the ethanol of 75%, and the percent of other ethanol is identical all therewith.
The Dendrobium officinale health product of auxiliary hyperglycemic of the present invention can make various dosage form, and described dosage form comprises hard capsule for oral administration, soft capsule, tablet or granule.Preferably hard capsule, main manifestations is: cover adverse drug abnormal smells from the patient, rapid-action, good absorbing.
The curative effect of the Dendrobium officinale health product of auxiliary hyperglycemic of the present invention is as follows,
Function: YIN nourishing and the production of body fluid promoting heat extraction.
Cure mainly: auxiliary hyperglycemic, to prevent diabetes.
Disease is seen: type ii diabetes, high blood glucose.
Usage: every day three times, each one.
Fang Xie: the generation of diabetes is not enough relevant to natural endowment, but acquired disposition also plays an important role, and has because of eating and drinking without temperance, the peppery rich thing of polyphagia oil, taste can not transporting, makes raw interior-heat, pents up dryness-transformation, consume impairment of YIN liquid, can not nourish spleen kidney and send out; Have because of Nervous and Mental Factors, stagnated QI transforming into fire, the bright body fluid that disappears forms.Its pathological change, the generally not outer deficiency of YIN and scorching two aspects, and reciprocal causation.Primary disease belongs to Chinese medicine and " to quench one's thirst " category, controls and should adopt Replenishing QI and nourishing YIN, the square medicine of clearing away heat and promoting production of body fluid.We's Herba Dendrobii YIN nourishing and the production of body fluid promoting heat extraction, Radix Panacis Quinquefolii boosting qi and nourishing yin promotes the production of body fluid, and is principal agent altogether.Radix Trichosanthis, Radix Puerariae clearing away heat and promoting production of body fluid are accessory drugs.Fructus Schisandrae Chinensis is sour and astringent, and the arresting sweating that promotes the production of body fluid is adjuvant.Full side's treating both the principal and secondary aspects of a disease, closes and with it, has good preventive and therapeutic effect to disease of quenching one's thirst.
One, know-why of the present invention and technical study:
1. alcohol extraction condition is preferred
1.1 adopt Orthogonal Experiment and Design screening alcohol extraction condition
In the preparation method of the Dendrobium officinale health product of auxiliary hyperglycemic of the present invention, Radix Puerariae and Fructus Schisandrae Chinensis adopt the method for alcohol extraction, now investigate solvent times amount, extraction time, concentration of alcohol, extraction time four factors to the impact of extracting.Take the Radix Puerariae of twice recipe quantity, Fructus Schisandrae Chinensis, extract by following factor level table, factor level table is in table 1.
The quadrature factor water-glass of table 1. alcohol extraction condition
Select puerarin content (mg/g) and dry cream amount (g) to be inspection target, data analysis is carried out to orthogonal test.Orthogonal calendar and the results are shown in Table 2.
The orthogonal arrangement that table 2. puerarin content (mg/g) and dry cream amount (g) are inspection target and result table
9 testing programs are provided in table.If No. 6 test is A
2b
3c
1d
2, i.e. 80% ethanol, 10 times amount reflux, extract, 2 times, each 1h, the rest may be inferred.
With dry cream amount for index, range analysis result D>B>A>C, namely influence factor put in order as extraction time > solvent times amount > concentration of alcohol > extraction time.Best of breed between each factor and level is A
2b
3c
3d
2, with C item for error term, carry out variance analysis further, the results are shown in Table 3.
Table 3. with dry cream heavily for the analysis of variance table of index
Each factor affects equal not statistically significant to experimental result.Extraction process best of breed is extraction twice, each 2h, and concentration of alcohol is 80%, and ethanol consumption is 10 times amount.
Take puerarin content as index, range analysis result B>A>D>C, namely influence factor put in order as solvent times amount > concentration of alcohol > extraction time > extraction time.Best of breed between each factor and level is A
2b
3c
2d
2, with C item for error term, carry out variance analysis further, the results are shown in Table 4.
Table 4. take puerarin content as the analysis of variance table of index
Each factor affects equal not statistically significant to experimental result.Extraction process best of breed is extraction twice, each 1.5h, and concentration of alcohol is 80%, and ethanol consumption is 10 times amount.
For the more orthogonal result of rational evaluation, find optimum extraction process, carry out comprehensive grading to two evaluation indexes, computational methods are as follows: in two indices, choose soprano respectively as 100 points, all the other press formulae discovery: score Y=weight × (100-peak+y
i), to two indices weight coefficient respectively for being 0.4 and 0.6.Pass through intuitive analysis, take comprehensive grading as index, show that each factor is B>A>D>C to extraction Influence on test result order, namely influence factor put in order as solvent times amount > concentration of alcohol > extraction time > extraction time.Best of breed between each factor and level is A
2b
3c
2d
2, with C item for error term, carry out variance analysis the results of analysis of variance further in table 5.
Table 5. take comprehensive grading as the analysis of variance table of index
Result shows, each factor is to experimental result not statistically significant, and extraction process best of breed is extraction twice, each 1.5h, and concentration of alcohol is 80%, and ethanol consumption is 10 times amount.
1.2 alcohol extraction demonstration tests
In order to verify the above results, taking Radix Puerariae and the Fructus Schisandrae Chinensis of twice recipe quantity, selecting A
2b
3c
2d
2condition carries out alcohol extraction demonstration test, the results are shown in Table 6.
Table 6. alcohol extraction demonstration test result
Conclusion: 3 times result of the test confirms: A
2b
3c
2d
2alcohol extraction condition conforms to substantially with orthogonal experiments, the factor such as industrial production cost and preparations shaping technique after considering, therefore by A
2b
3c
2d
2i.e. 80% ethanol 10 times amount, extracts twice, each 1.5 hours, is defined as best alcohol extraction process condition.
2. abstract methods is preferred
2.1 adopt Orthogonal Experiment and Design screening abstract methods
In prescription, Radix Trichosanthis adopts the method for water extraction, now investigates solvent times amount, extraction time, extraction time three factors to the impact of extracting.Take the Radix Trichosanthis of twice recipe quantity and Radix Puerariae, the alcohol extraction medicinal residues of Fructus Schisandrae Chinensis extract by following factor level table, factor level table is in table 7.
The quadrature factor water-glass of table 7. abstract methods
The present invention selects THFPS content (mg/g) and dry cream amount (g) to be inspection target, carries out data analysis to orthogonal test.Orthogonal calendar and the results are shown in Table 8.
Table 8. is with THFPS content (mg/g) and dry cream amount (g) be inspection target orthogonal arrangement and result table
9 testing programs are provided in table.If No. 6 test is A
2b
3c
1, i.e. the water reflux, extract, 1 time of 8 times amount, each 2h, the rest may be inferred.
With dry cream amount for index, range analysis result B>A>C, namely influence factor puts in order as extraction time > solvent times amount > extraction time.Best of breed between each factor and level is A
2b
3c
3, with D item for error term, carry out variance analysis further, the results are shown in Table 9.
The analysis of variance table that table 9. is index with dry cream amount
Each factor affects equal not statistically significant to experimental result.Extraction process best of breed is extraction 3 times, each 2h, and water consumption is 8 times amount.
With THFPS content for index, range analysis result C>A>B, namely influence factor put in order as extraction time > solvent times amount > extraction time.Best of breed between each factor and level is A
3b
3c
3, with D item for error term, carry out variance analysis further, the results are shown in Table 10.
The analysis of variance table that table 10. is index with THFPS content
Each factor affects equal not statistically significant to experimental result.Extraction process best of breed is extraction 3 times, each 2h, and water consumption is 10 times amount.
For the more orthogonal result of rational evaluation, find optimum extraction process, carry out comprehensive grading to two evaluation indexes, computational methods are as follows: in two indices, choose soprano respectively as 100 points, all the other press formulae discovery: score Y=weight × (100-peak+y
i), to two indices weight coefficient respectively for being 0.4 and 0.6.Pass through intuitive analysis, take comprehensive grading as index, show that each factor is C>B>A to extraction Influence on test result order, namely influence factor puts in order as extraction time > extraction time > solvent times amount.Best of breed between each factor and level is A
3b
3c
3, with D item for error term, carry out variance analysis the results of analysis of variance further in table 11.
Table 11. take comprehensive grading as the analysis of variance table of index
Result shows, each factor is to experimental result not statistically significant, and extraction process best of breed is: extract 3 times, each 2h, water consumption is 10 times amount.
2.2 water extraction demonstration tests
In order to verify the above results, taking the Radix Trichosanthis of twice recipe quantity and the alcohol extraction medicinal residues of Radix Puerariae and Fructus Schisandrae Chinensis, selecting A
3b
3c
3condition carries out water extraction demonstration test, the results are shown in Table 12.
Table 12. alcohol extraction demonstration test result
Conclusion: 3 times result of the test confirms: A
3b
3c
3abstract methods conforms to substantially with orthogonal experiments, the factor such as industrial production cost and preparations shaping technique after considering, therefore by A
3b
3c
3namely extract 3 times, each 2 hours, water consumption was 10 times amount, was defined as best extraction process by water condition.
Two, efficacy study:
The multifactorial metabolic disease of diabetes to be one group with hyperglycemia be feature, with inherited genetic factors and bad border factor closely related, the cause of disease is very complicated.The high fatality rate of its hazardness mainly caused by diabetic complication (as diabetes cardiovascular and cerebrovascular disease, diabetic nephropathy and retinopathy etc.).Primary disease is mainly in old people, has a strong impact on quality of life and the work of patient.Diabetes are a kind of lifelong disease at present, still lack radical treatment method.The prescription of these health product is through repeated screening, the Empirical formula of summing up application, and zoopery and human experiment experiment also show to achieve good treatment curative effect to primary disease.This Dendrobium officinale health product is in the experimentally observation of hypoglycemic activity of diabetes rat model and human experiment and research.Be reported as follows:
Test by " health food inspection and assessment technical specification " (version in 2003) auxiliary hyperglycemic function method of inspection.
1. zoopery
1.1 laboratory animals: kind healthy male mice (26 ± 2g) Shanghai, SPF level Kunming western pul-Bi Kai laboratory animal company limited breeds.
1.2 reagent: alloxan (Alloxan Monohydrate) A7413, Beijing Mei Kemei biotechnology development corporation, Ltd.; Metformin hydrochloride 2013010711, the safe Pharmaceutical responsibility company limited in east, Tangyin, White Cloud Mountain; Dendrobium officinale health product (semi-finished product).
1.3 instrument
Easy blood glucose meter (blood sugar detection reagent paper) Johnson Co. is surely selected by Johnson & Johnson
1.4 methods and result
1.4.1 the experiment of modeling fasting glucose is reduced
Select hyperglycemia model animal to divide into groups by the blood sugar level of fasting 3-5h, select 1 model control group at random, 1 positive controls and 3 dosage groups (between group, difference is not more than 1.1mmol/L), often organize mice 12.Dosage group gives variable concentrations given the test agent, be respectively 0.1g/kg, 0.2g/kg, 0.4g/kg, model control group gives same volume solvent, positive controls gives 0.2g/kg metformin hydrochloride, every day gavage 1 time, continuous 30 days, survey fasting blood sugar (fasting is with before experiment), more each treated animal blood glucose value and blood glucose decline percentage rate.Blood glucose value * 100% before blood glucose decline percentage rate=(before experiment the rear blood glucose value of blood glucose value-experiment)/experiment.The results are shown in Table 13.
Table 13. is on the impact of modeling mice fasting glucose
* P < 0.05***P < 0.001 is compared with model control group
Table 13 result shows, the Dendrobium officinale health product of auxiliary hyperglycemic of the present invention has the effect obviously reducing modeling mice fasting glucose.
1.4.2 reduce fasting glucose experiment
Healthy adult animal is selected to divide into groups by the blood sugar level of fasting 3-5h, select 1 matched group at random, 1 positive controls and 1 are by test product group, matched group gives same volume solvent, positive controls gives 0.2g/kg metformin hydrochloride, give high dose given the test agent (0.4g/kg) by test product group, operate the same joint 1.4.1.The results are shown in Table 14.
Table 14. is on the impact of healthy mice fasting glucose
* * * P < 0.001 is compared with matched group
Table 14 result shows, the Dendrobium officinale health product of auxiliary hyperglycemic of the present invention has the effect obviously reducing healthy mice fasting glucose.
1.4.3 carbohydrate tolerance experiment
Hyperglycemia model animal fasting 3-5h, dosage group gives variable concentrations given the test agent, be respectively 0.1g/kg, 0.2g/kg, 0.4g/kg, model control group gives same volume solvent, positive controls gives 0.2g/kg metformin hydrochloride, oral administration of glucose 2.0g/kg after 15-20min, measure to after glucose 0,0.5, the blood glucose value of 2h.The results are shown in Table 15.
Table 15. is on the impact of modeling glucose tolerance in mice
* P < 0.05, * * P < 0.01, * * * P < 0.001 is compared with model control group
Shown by table 15 result, the Dendrobium officinale health product of auxiliary hyperglycemic of the present invention obviously can reduce modeling mice post-prandial glycemia.
2 human experiment experiments
2.1 experimenters select and grouping
Select the state of an illness after diet control or oral antidiabetic drug treatment more stable, do not need to change medicine species and dosage, only take adult type ii diabetes patient 120 example of maintenance dose, wherein man 72 example, female 48 example, age 28-60, is divided into test-meal group and matched group (adopting two kinds of control design between self and group) by random blind, often organizes 60 examples.
2.2 Therapeutic Method
Press sex, age, different labor intensity etc. to every experimenter before experiment, and put somebody on a diet with reference to original living habit, adhere to diet control during test-meal, medicament categories and the dosage for the treatment of diabetes are constant.Test-meal group is on the basis of taking medicine, and every day 3 times, take given the test agent for each 1, matched group takes the placebo of Isodose on the basis of taking medicine, and given the test agent gives 30 days.
2.3 observation index
2.3.1 observation of symptoms
Experiment Patients Before And After observation of symptoms comprises the main clinic symptoms such as thirsty polydipsia, polyorexia, fatigue and weakness, polyuria, by mild symptoms multiple integral, and statistics integrated value, and observe clinical symptoms improvement rate (improving 1 to be divided into effectively).Table 16 is the clinical symptoms table of integrals
The table 16. clinical symptoms table of integrals
Result: test-meal group 93% patient clinical symptom makes moderate progress, matched group 2% patient clinical symptom is improved.
2.3.2 fasting glucose
Before and after test-meal, fasting blood sugar and blood glucose decline percentage rate, the results are shown in Table 17.
Table 17. is on the impact of diabetics fasting glucose
* * * P < 0.001 is compared with matched group
Table 17 result shows, the Dendrobium officinale health product of auxiliary hyperglycemic of the present invention has the effect obviously reducing diabetics fasting glucose.
2.3.3 2h-plasma glucose
2h blood glucose value and blood glucose decline percentage rate after edible 100g fine powder steamed bread after test-meal, the results are shown in Table 18.
Table 18. is on the impact of diabetics post-prandial glycemia
* * * P < 0.001 is compared with matched group
Table 18 result shows, the Dendrobium officinale health product of auxiliary hyperglycemic of the present invention has the effect of obviously falling diabetics post-prandial glycemia.
2.3.4 glucose in urine
Qualitative with empty stomach urina sanguinis, by-, ± ,+, ++, +++, ++++long-pending 0,0.5,1,2,3,4 point respectively, before and after test-meal, add up integrated value.
Result: test-meal group 96% patient on an empty stomach urina sanguinis integration reduces, matched group 1% patient on an empty stomach urina sanguinis integration reduces.
2.4 discuss
All can be illustrated by above zoopery and human experiment experimental result, the Dendrobium officinale health product of auxiliary hyperglycemic of the present invention has obvious hypoglycemic activity, particularly human experiment experiment alleviates patient clinical symptom (polydipsia, polyphagia, polyuria), improves the life quality of patient; And in experimentation, there is not any toxic and side effects and untoward reaction.The each medical material nature and flavor of Dendrobium officinale health product of auxiliary hyperglycemic of the present invention are gentle, preparation method is simple, dosage form taking convenience; Therefore, can be used for auxiliary treatment type ii diabetes.
3 compared with the prior art
3.1
Patent (CN01132049.4) relates to a kind of Chinese medicine preparation for the treatment of diabetes, the pharmaceutical preparation be made up of Fructus Schisandrae Chinensis supercritical extract and the 99.9%-0% pharmaceutic adjuvant of 0.1%-100%.Patent is compared therewith, invention increases Herba Dendrobii, Radix Panacis Quinquefolii, Radix Puerariae, Radix Trichosanthis four Chinese medicine material, wherein Herba Dendrobii, Radix Panacis Quinquefolii are first-class medical material, have the effect of nourishing YIN and clearing away heat, tonifying QI to produce body fluid, have good auxiliary therapeutic action to the scorching diabetes for pathogenesis of the deficiency of YIN; Radix Puerariae is one of " four large panacea " for the treatment of diabetes, and hypoglycemic activity is obvious; Also clear and definite hypoglycemic activity is had according to the THFPS in document Radix Trichosanthis.
3.2
Herba Dendrobii Radix Panacis Quinquefolii buccal tablets that a kind of applicable diabetics of patent (CN201310073256.0) is taken and preparation method thereof, is made up of the fresh bar of Herba Dendrobii, American ginseng fresh root and sugar material.Patent is compared therewith, and in the present invention, Radix Trichosanthis, Radix Puerariae clearing away heat and promoting production of body fluid, have obvious hypoglycemic activity; Fructus Schisandrae Chinensis is sour and astringent promote the production of body fluid arresting sweating, moisturize heat extraction, full side's treating both the principal and secondary aspects of a disease, has good prevention and auxiliary therapeutic action to type ii diabetes; Further, both technique and parameter are compared, in table 20.
Comparing of table 20. patent (CN201310073256.0) and present invention process and parameter
As can be seen from above relatively, except the difference of prescription, this patent and the present invention also have some differences in technique.Mainly mode that is dry and that pulverize is different, this patent flour broken iron Herba Dendrobii and Radix Panacis Quinquefolii adopt nano-pulverization technology, after nano-pulverization, medical material particle diameter 0.1-100nm is less than micronizing medical material particle diameter 10 μm, but the ratio that modern crushing technology reaches tens nanometer is also lower, can reach that nano level to be generally mineral powder more, for fiber-like plant, during operation, temperature rising can cause viscosity to increase, and pulverizes large nanoscale more difficult; In addition, the small-size effect, skin effect, quantum size effect etc. of nanoparticle make it have special physicochemical property, biological activity and biodynamics process, thus produce various potential danger to human body.The present invention adopts Vacuum Freezing & Drying Technology to have the following advantages compared with traditional dry technology to the drying of the fresh bar of Herba Dendrobii: carry out under low temperature; loss is applicable to heat-sensitive substance less; original structure is maintained after drying; concentration phenomena can not be there is; and drying is carried out protecting some oxidizable compositions in a vacuum, and drying thoroughly increases shelf life of products.In sum, the present invention has more advantage in technique.
3.3 side's of tearing open efficacy studies
We split raw material components of the present invention and carry out carbohydrate tolerance experiment, and respectively split the function of reducing blood sugar of prescription further, seven prescriptions of fractionation are respectively:
Prescription 1: Herba Dendrobii fine powder+Radix Panacis Quinquefolii fine powder;
Prescription 2: Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear paste;
Prescription 3: Radix Trichosanthis water extraction clear paste;
Prescription 4: Herba Dendrobii fine powder+Radix Panacis Quinquefolii fine powder+Radix Puerariae alcohol extraction clear paste;
Prescription 5: Herba Dendrobii fine powder+Radix Panacis Quinquefolii fine powder+Radix Trichosanthis water extraction clear paste;
Prescription 6: Herba Dendrobii fine powder+Radix Panacis Quinquefolii fine powder+Radix Puerariae alcohol extraction clear paste+Radix Trichosanthis water extraction clear paste;
Prescription 7: Quan Fang
Experimental procedure is with 1.4.2 carbohydrate tolerance experiment under Efficacy experiments item, and each prescription all gives the given the test agent of 0.2g/kg, and model control group gives same volume solvent, and positive controls gives 0.2g/kg metformin hydrochloride, the results are shown in Table 19.
The each prescription of table 19. is on the impact of modeling glucose tolerance in mice
* P < 0.05, * * P < 0.01, * * * P < 0.001 is compared with matched group
The hypoglycemic effect of prescription 7 is better than other prescription as can be seen from the above table, of great advantage to the treatment of diabetes.
In sum, compared with prior art, the beneficial effect that possesses of the present invention:
1, square medicine
Clinical practice under the next free guidance of traditional Chinese medicine theory of raw material prescription of the Dendrobium officinale health product of auxiliary hyperglycemic of the present invention, prescription drugs monosodium glutamate refines, with strong points.Described raw material prescription Chinese medicine enriching yin boost qi, clearing away heat and promoting production of body fluid, effective ingredient is easy to absorb, and is conducive to playing drug effect; In described raw material prescription, each medical material is integration of edible and medicinal herbs medical material, has no side effect.
The advantage of the Dendrobium officinale health product of auxiliary hyperglycemic of the present invention is: 1, flavour of a drug are few, refining formula; 2, each medical material is integration of edible and medicinal herbs medical material and has no side effect; 3, endangered species of wild fauna and flora is not had; 4, economize on resources; Conveniently prepare dosage form.
The application in the process of prevention and adjuvant therapy of diabetes of the Dendrobium officinale health product of auxiliary hyperglycemic of the present invention, oral every day three times, each one.
2, preparation method
In 2.1 the present invention, Herba Dendrobii adopts Vacuum Freezing & Drying Technology, Freeze Drying Technique is as an important Chinese medicine new technique, it has the irreplaceable advantage of other dry technology: 1. lyophilization is carried out at low temperatures, therefore for the material particularly suitable of much thermal sensitivity.2., time dry at low temperatures, some volatile ingredients loss in material is very little.3., in freezing dry process, the effect of microbial growth and enzyme cannot be carried out, and therefore can keep original character.4. owing to carrying out drying under the state freezed, therefore volume is almost constant, maintains original structure, concentration phenomena can not occur.5. dried material, dissolves rapidly and completely after adding water, almost recovers original character immediately.6. because drying is carried out under vacuo, oxygen is few, and therefore some oxidizable materials obtain protection.7. the dry moisture content getting rid of more than 95-99%, make dry after product to preserve for a long time and unlikely rotten.Because topmost active component is exactly Herba Dendrobii active polysaccharide in Herba Dendrobii, the height of polyoses content directly affects the health-care effect of Herba Dendrobii, adopt Freeze Drying Technique that Herba Dendrobii active polysaccharide can be avoided to lose because of pyrolytic, remain the functional component of Herba Dendrobii better.
Superfine communication technique is adopted: micronizing can be processed into the even nano level micropowder of micron raw material in 2.2 the present invention, medical material after micronizing not only can complete reservation nutritional labeling, improve mouthfeel, and the stripping of effective ingredient in medical material is improved by cell wall breaking, improve medical material utilization rate, also can conveniently adjust and improve the quality of products.In addition also there is following characteristics: 1. control Powder Particle Size scope below 10 μm, powder particle diameter is little, is evenly distributed, and profile is neat, and quality is good, is convenient to application; 2. equipment reflux can be pulverized in the granule auto-returned vortex cavity after selecting crude drugs with winnower again, and pulverize speed fast, the time is short, shortens the production cycle, increases work efficiency; 3. there is evaporation to dewater and cold-hot wind functions/drying, without superheating phenomenon, be conducive to retaining powder bioactive ingredients; 4. there is fresh-keeping effect to the material of thermal sensitivity, armaticity, the effective active of preparation can be significantly improved; 5. multifilament, elasticity, viscous material also can process desired level, improve the dissolution rate of effective ingredient, are conducive to absorbing; 6. there is certain sterilization functions, pollute little, improve product health level, meet GMP requirement.
The Dendrobium officinale health product of auxiliary hyperglycemic of the present invention is with in the long-term practice of Chinese medicine prevention, adjuvant therapy of diabetes, through repeated screening, sums up the Empirical formula of application.Applicating modern times technology, achieves good curative effect.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in further detail.
Embodiment one:
A preparation method for the Dendrobium officinale health product of auxiliary hyperglycemic, comprises the steps:
(1) raw material takes: count by weight, and take following raw material components, raw material components is as follows: Herba Dendrobii 40 parts, Radix Panacis Quinquefolii 1 part, Radix Puerariae 3 parts, Radix Trichosanthis 3 parts and Fructus Schisandrae Chinensis 1.5 parts.
(2) process of the fresh bar of Herba Dendrobii: the fresh bar of Herba Dendrobii step (1) taken is cleaned, drain after, drop in Chinese medicine grinder and be ground into the long Herba Dendrobii segment of 1-2cm; Herba Dendrobii segment is carried out vacuum lyophilization, then carries out micronizing, obtain Herba Dendrobii fine powder;
(3) process of Radix Panacis Quinquefolii: the Radix Panacis Quinquefolii that step (1) takes is placed in the hot air circulation drying oven that temperature is less than 60 DEG C and carries out drying, then carry out micronizing, obtain Radix Panacis Quinquefolii fine powder;
(4) alcohol extraction:
Get Radix Puerariae and Fructus Schisandrae Chinensis that step (1) takes, add Radix Puerariae and Fructus Schisandrae Chinensis gross weight 10 times of 80% ethanol, stirring and refluxing extracts twice, each 1.5 hours, and backflow leaves standstill more than 12 hours, Aspirate supernatant, obtain Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear liquid, merge the alcohol extraction stillness of night, become Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear paste for subsequent use Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear liquid concentrating under reduced pressure, Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction medicinal residues are separately used;
(5) water extraction:
Get the Radix Trichosanthis that step (1) takes, add the Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction medicinal residues that step (3) obtains, add the water of Radix Trichosanthis and Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction medicinal residues gross weight 10 times again, stirring and refluxing extracts 3 times, each 2 hours, backflow left standstill more than 12 hours, Aspirate supernatant, obtain Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction clear liquid, Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction clear liquid concentrating under reduced pressure is become Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction clear paste;
(6) dry:
Be dried to dry cream Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear paste is less than the environment of 60 DEG C in temperature under, then be ground into Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction fine powder;
Be dried to dry cream Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction clear paste is less than the environment of 60 DEG C in temperature under, then be ground into Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction fine powder;
Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction fine powder and Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction fine powder were the fine powder that 80 mesh sieves obtain.
(7) prepare burden:
Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction fine powder 1 part of mix homogeneously that the Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction fine powder 1 part that the Radix Panacis Quinquefolii fine powder 1 part that the Herba Dendrobii fine powder 1 part step (2) obtained, step (3) obtain, step (6) obtain and step (6) obtain.
When making finished product, can also comprise the steps:
(7) microcrystalline Cellulose 0.49 part and magnesium stearate 0.01 part is added again, mix homogeneously.
(8) capsule-filling: get No. 0 capsule, loading amount is 0.45g/ grain, carries out fill.
The relative density of described Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear paste at 60 DEG C is 1.15 ~ 1.25.
The relative density of described RADIX PUERARIAE Fructus Schisandrae Chinensis water extraction clear paste at 60 DEG C is 1.15 ~ 1.25.
Embodiment two:
A preparation method for the Dendrobium officinale health product of auxiliary hyperglycemic, comprises the steps:
(1) raw material takes: count by weight, takes following raw material components, the fresh bar of Herba Dendrobii 35 parts, Radix Panacis Quinquefolii 1 part, Radix Puerariae 2 parts, Radix Trichosanthis 2 parts and Fructus Schisandrae Chinensis 1 part;
(2) process of the fresh bar of Herba Dendrobii: the fresh bar of Herba Dendrobii step (1) taken is cleaned, drain after, drop in Chinese medicine grinder and be ground into the long Herba Dendrobii segment of 1-2cm; Herba Dendrobii segment is carried out vacuum lyophilization, then carries out micronizing, obtain Herba Dendrobii fine powder;
(3) process of Radix Panacis Quinquefolii: the Radix Panacis Quinquefolii that step (1) takes is placed in the hot air circulation drying oven that temperature is less than 60 DEG C and carries out drying, then carry out micronizing, obtain Radix Panacis Quinquefolii fine powder;
(4) alcohol extraction:
Get Radix Puerariae and Fructus Schisandrae Chinensis that step (1) takes, add 75% ethanol of Radix Puerariae and Fructus Schisandrae Chinensis gross weight 8 times, stirring and refluxing extracts 2 times, each 1.3 hours, backflow left standstill more than 12 hours, Aspirate supernatant, obtain Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear liquid, merge alcohol extraction clear liquid, become Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear paste for subsequent use Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear liquid concentrating under reduced pressure, Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction medicinal residues are separately used;
(5) water extraction:
Get the Radix Trichosanthis that step (1) takes, add the Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction medicinal residues that step (3) obtains, add the water of Radix Trichosanthis and Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction medicinal residues gross weight 8 times again, stirring and refluxing extracts 2 times, each 1.8 hours, backflow left standstill more than 12 hours, Aspirate supernatant, obtain Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction clear liquid, Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction clear liquid concentrating under reduced pressure is become Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction clear paste;
(6) dry:
Be dried to dry cream Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear paste is less than the environment of 60 DEG C in temperature under, then be ground into Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction fine powder;
Be dried to dry cream Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction clear paste is less than the environment of 60 DEG C in temperature under, then be ground into Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction fine powder;
Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction fine powder and Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction fine powder were the fine powder that 140 mesh sieves obtain.
(7) prepare burden:
Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction fine powder 1 part of mix homogeneously that the Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction fine powder 2 parts that the Radix Panacis Quinquefolii fine powder 1.5 parts that the Herba Dendrobii fine powder 2 parts step (2) obtained, step (3) obtain, step (6) obtain and step (6) obtain.
When making finished product, can also comprise the steps:
(8) microcrystalline Cellulose 0.49 part and magnesium stearate 0.01 part is added again, mix homogeneously.
(9) tabletting: diameter 10mm punch die, tabletting, the heavy 0.3g/ sheet of sheet;
(10) coating: Opadry film coating and get final product.
Embodiment three:
A preparation method for the Dendrobium officinale health product of auxiliary hyperglycemic, comprises the steps:
(1) raw material takes: count by weight, takes following raw material components, the fresh bar of Herba Dendrobii 45 parts, Radix Panacis Quinquefolii 2 parts, Radix Puerariae 4 parts, Radix Trichosanthis 4 parts and Fructus Schisandrae Chinensis 2 parts;
(2) process of the fresh bar of Herba Dendrobii: the fresh bar of Herba Dendrobii step (1) taken is cleaned, drain after, drop in Chinese medicine grinder and be ground into the long Herba Dendrobii segment of 1-2cm; Herba Dendrobii segment is carried out vacuum lyophilization, then carries out micronizing, obtain Herba Dendrobii fine powder;
(3) process of Radix Panacis Quinquefolii: the Radix Panacis Quinquefolii that step (1) takes is placed in the hot air circulation drying oven that temperature is less than 60 DEG C and carries out drying, then carry out micronizing, obtain Radix Panacis Quinquefolii fine powder;
(4) alcohol extraction:
Get Radix Puerariae and Fructus Schisandrae Chinensis that step (1) takes, add 80% ethanol of Radix Puerariae and Fructus Schisandrae Chinensis gross weight 10 times, stirring and refluxing extracts 3 times, each 1.8 hours, backflow left standstill more than 12 hours, Aspirate supernatant, obtain Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear liquid, merge the alcohol extraction stillness of night, become Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear paste for subsequent use Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear liquid concentrating under reduced pressure, Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction medicinal residues are separately used;
(5) water extraction:
Get the Radix Trichosanthis that step (1) takes, add the Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction medicinal residues that step (3) obtains, add the water of Radix Trichosanthis and Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction medicinal residues gross weight 10 times again, stirring and refluxing extracts 3 times, each 2.2 hours, backflow left standstill more than 12 hours, Aspirate supernatant, obtain Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction clear liquid, Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction clear liquid concentrating under reduced pressure is become Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction clear paste;
(6) dry:
Be dried to dry cream Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear paste is less than the environment of 60 DEG C in temperature under, then be ground into Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction fine powder;
Be dried to dry cream Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction clear paste is less than the environment of 60 DEG C in temperature under, then be ground into Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction fine powder;
Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction fine powder and Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction fine powder were the fine powder that 120 mesh sieves obtain.
(7) prepare burden:
Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction fine powder 1.2 parts of mix homogeneously that the Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction fine powder 1 part that the Radix Panacis Quinquefolii fine powder 2 parts that the Herba Dendrobii fine powder 1.5 parts step (2) obtained, step (3) obtain, step (6) obtain and step (6) obtain.
When making finished product, can also comprise the steps:
(8) 3.99 parts, dextrin, steviosin 0.01 part, soft material made by 80% ethanol, crosses 10 mesh sieves, makes wet granular.
(9) dry, granulate: put≤60 DEG C of dryings, with 12 mesh sieve granulate.
(10) subpackage: use polyethylene composite film subpackage, loading amount is 6g/ bag and get final product.
Embodiment four:
A preparation method for the Dendrobium officinale health product of auxiliary hyperglycemic, comprises the steps:
(1) raw material takes: count by weight, takes following raw material components, the fresh bar of Herba Dendrobii 38 parts, Radix Panacis Quinquefolii 1.2 parts, Radix Puerariae 3 parts, Radix Trichosanthis 2.5 parts and Fructus Schisandrae Chinensis 1.8 parts;
(2) process of the fresh bar of Herba Dendrobii: the fresh bar of Herba Dendrobii step (1) taken is cleaned, drain after, drop in Chinese medicine grinder and be ground into the long Herba Dendrobii segment of 1-2cm; Herba Dendrobii segment is carried out vacuum lyophilization, then carries out micronizing, obtain Herba Dendrobii fine powder;
(3) process of Radix Panacis Quinquefolii: the Radix Panacis Quinquefolii that step (1) takes is placed in the hot air circulation drying oven that temperature is less than 60 DEG C and carries out drying, then carry out micronizing, obtain Radix Panacis Quinquefolii fine powder;
(4) alcohol extraction:
Get Radix Puerariae and Fructus Schisandrae Chinensis that step (1) takes, add 78% ethanol of Radix Puerariae and Fructus Schisandrae Chinensis gross weight 9 times, stirring and refluxing extracts 2 times, each 1.6 hours, backflow left standstill more than 12 hours, Aspirate supernatant, obtain Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear liquid, merge the alcohol extraction stillness of night, become Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear paste for subsequent use Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear liquid concentrating under reduced pressure, Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction medicinal residues are separately used;
(5) water extraction:
Get the Radix Trichosanthis that step (1) takes, add the Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction medicinal residues that step (3) obtains, add the water of Radix Trichosanthis and Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction medicinal residues gross weight 9 times again, stirring and refluxing extracts 3 times, each 1.8 hours, backflow left standstill more than 12 hours, Aspirate supernatant, obtain Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction clear liquid, Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction clear liquid concentrating under reduced pressure is become Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction clear paste;
(6) dry:
Be dried to dry cream Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear paste is less than the environment of 60 DEG C in temperature under, then be ground into Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction fine powder;
Be dried to dry cream Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction clear paste is less than the environment of 60 DEG C in temperature under, then be ground into Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction fine powder;
Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction fine powder and Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction fine powder were the fine powder that 100 mesh sieves obtain.
(7) prepare burden:
Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction fine powder 2 parts of mix homogeneously that the Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction fine powder 1.8 parts that the Radix Panacis Quinquefolii fine powder 1 part that the Herba Dendrobii fine powder 1.5 parts step (2) obtained, step (3) obtain, step (6) obtain and step (6) obtain.
Claims (11)
1. a Dendrobium officinale health product for auxiliary hyperglycemic, is characterized in that, counts by weight, and raw material components is as follows: the fresh bar of Herba Dendrobii 35 ~ 45 parts, Radix Panacis Quinquefolii 1 ~ 2 part, Radix Puerariae 2 ~ 4 parts, Radix Trichosanthis 2 ~ 4 parts and Fructus Schisandrae Chinensis 1 ~ 2 part.
2. the Dendrobium officinale health product of auxiliary hyperglycemic as claimed in claim 1, it is characterized in that, count by weight, raw material components is as follows: Herba Dendrobii 40 parts, Radix Panacis Quinquefolii 1 part, Radix Puerariae 3 parts, Radix Trichosanthis 3 parts and Fructus Schisandrae Chinensis 1.5 parts.
3. a preparation method for the Dendrobium officinale health product of auxiliary hyperglycemic, is characterized in that, comprises the steps:
(1) raw material takes: count by weight, takes following raw material components respectively, the fresh bar of Herba Dendrobii 35 ~ 45 parts, Radix Panacis Quinquefolii 1 ~ 2 part, Radix Puerariae 2 ~ 4 parts, Radix Trichosanthis 2 ~ 4 parts and Fructus Schisandrae Chinensis 1 ~ 2 part;
(2) process of the fresh bar of Herba Dendrobii: the fresh bar of Herba Dendrobii step (1) taken is cleaned, drain after, drop in Chinese medicine grinder and be ground into the long Herba Dendrobii segment of 1-2cm; Herba Dendrobii segment is carried out vacuum lyophilization, then carries out micronizing, obtain Herba Dendrobii fine powder;
(3) process of Radix Panacis Quinquefolii: the Radix Panacis Quinquefolii that step (1) takes is placed in the hot air circulation drying oven that temperature is less than 60 DEG C and carries out drying, then carry out micronizing, obtain Radix Panacis Quinquefolii fine powder;
(4) alcohol extraction:
Get Radix Puerariae and Fructus Schisandrae Chinensis that step (1) takes, add 75 ~ 80% ethanol of Radix Puerariae and Fructus Schisandrae Chinensis gross weight 8 ~ 10 times, stirring and refluxing is extracted 2 ~ 3 times, each 1.3 ~ 1.8 hours, backflow left standstill more than 12 hours, Aspirate supernatant, obtain Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear liquid, merge supernatant, become Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear paste for subsequent use Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear liquid concentrating under reduced pressure, Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction medicinal residues are separately preserved;
(5) water extraction:
Get the Radix Trichosanthis that step (1) takes, add the Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction medicinal residues that step (4) obtains, add the water of Radix Trichosanthis and Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction medicinal residues gross weight 8 ~ 10 times again, stirring and refluxing is extracted 2 ~ 3 times, each 1.8 ~ 2.2 hours, backflow left standstill more than 12 hours, Aspirate supernatant, obtain Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction clear liquid, Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction clear liquid concentrating under reduced pressure is become Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction clear paste;
(6) dry:
Be dried to dry cream Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear paste is less than the environment of 60 DEG C in temperature under, then be ground into Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction fine powder;
Be dried to dry cream Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction clear paste is less than the environment of 60 DEG C in temperature under, then be ground into Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction fine powder;
(7) prepare burden:
Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction fine powder 1 ~ 2 part of mix homogeneously that the Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction fine powder 1 ~ 2 part that the Radix Panacis Quinquefolii fine powder 1 ~ 2 part that the Herba Dendrobii fine powder 1 ~ 2 part step (2) obtained, step (3) obtain, step (6) obtain and step (6) obtain, obtains the Dendrobium officinale health product of described auxiliary hyperglycemic.
4. the preparation method of the Dendrobium officinale health product of auxiliary hyperglycemic as claimed in claim 3, it is characterized in that, the relative density of Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction clear paste 60 DEG C time is 1.15 ~ 1.25.
5. the preparation method of the Dendrobium officinale health product of auxiliary hyperglycemic as claimed in claim 3, it is characterized in that, the relative density of Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction clear paste 60 DEG C time is 1.15 ~ 1.25.
6. the preparation method of the Dendrobium officinale health product of auxiliary hyperglycemic as claimed in claim 3, it is characterized in that, the process conditions of alcohol extraction are: add Radix Puerariae and Fructus Schisandrae Chinensis gross weight 10 times amount 80% ethanol, and stirring and refluxing extracts twice, each 1.5 hours.
7. the preparation method of the Dendrobium officinale health product of auxiliary hyperglycemic as claimed in claim 3, it is characterized in that, the process conditions of water extraction are: the water adding Radix Trichosanthis and Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction medicinal residues gross weight 10 times amount, stirring and refluxing extracts 3 times, each 2 hours.
8. the preparation method of the Dendrobium officinale health product of auxiliary hyperglycemic as claimed in claim 3, it is characterized in that, the process conditions of batching are: Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction fine powder 1 part of mix homogeneously that the Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction fine powder 1 part that the Radix Panacis Quinquefolii fine powder 1 part that the Herba Dendrobii fine powder 1 part step (2) obtained, step (3) obtain, step (6) obtain and step (6) obtain.
9. the preparation method of hypoglycemic Chinese medicine composition as claimed in claim 3, is characterized in that: the Dendrobium officinale health product of described auxiliary hyperglycemic conveniently technology of preparing is prepared into tablet, granule, hard capsule or soft capsule.
10. the preparation method of hypoglycemic Chinese medicine composition as claimed in claim 3, is characterized in that: Radix Puerariae Fructus Schisandrae Chinensis alcohol extraction fine powder and Radix Trichosanthis Radix Puerariae Fructus Schisandrae Chinensis water extraction fine powder were the fine powder that 80 ~ 140 mesh sieves obtain.
The preparation method of 11. a kind of hypoglycemic Chinese medicine compositions as claimed in claim 1 or 2, is characterized in that: described Chinese medicine composition is preparing the application in blood sugar lowering class medicine.
Priority Applications (1)
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105535553A (en) * | 2016-03-10 | 2016-05-04 | 厦门塔斯曼生物工程有限公司 | Formula for regulating blood sugar of human body |
| CN105533389A (en) * | 2016-01-28 | 2016-05-04 | 广西大学 | Dendrobium officinale and carambola fruit solid drink particle and preparation method thereof |
| CN106344808A (en) * | 2016-08-31 | 2017-01-25 | 浙江天方科技股份有限公司 | Compound dendrobium officinale health care product capable of assisting in reducing blood sugar |
| CN107997170A (en) * | 2017-11-30 | 2018-05-08 | 江苏康缘药业股份有限公司 | A kind of composition with hypolipemic function and preparation method thereof |
| CN108096474A (en) * | 2018-01-19 | 2018-06-01 | 郑州思邈堂生物科技有限公司 | Reduce by three high particles and preparation method thereof |
| CN114748582A (en) * | 2021-01-08 | 2022-07-15 | 遵义医科大学 | Traditional Chinese medicine composition for reducing blood sugar and application thereof |
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| CN1371724A (en) * | 2002-03-13 | 2002-10-02 | 于炳信 | Medicine for treating diabetes |
| CN102178848A (en) * | 2011-05-04 | 2011-09-14 | 湖南龙石山铁皮石斛基地有限公司 | Traditional Chinese medicinal dendrobium officinale preparation and preparation method thereof |
| CN103230538A (en) * | 2013-04-25 | 2013-08-07 | 浙江亚林生物科技股份有限公司 | Fresh dendrobium officinale American ginseng particle and preparation method thereof |
| US20140288186A1 (en) * | 2013-03-19 | 2014-09-25 | Tunghai University | Method for lowering blood glucose and method for treating hepatic disease, obesity and diabetes |
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| CN1371724A (en) * | 2002-03-13 | 2002-10-02 | 于炳信 | Medicine for treating diabetes |
| CN102178848A (en) * | 2011-05-04 | 2011-09-14 | 湖南龙石山铁皮石斛基地有限公司 | Traditional Chinese medicinal dendrobium officinale preparation and preparation method thereof |
| US20140288186A1 (en) * | 2013-03-19 | 2014-09-25 | Tunghai University | Method for lowering blood glucose and method for treating hepatic disease, obesity and diabetes |
| CN103230538A (en) * | 2013-04-25 | 2013-08-07 | 浙江亚林生物科技股份有限公司 | Fresh dendrobium officinale American ginseng particle and preparation method thereof |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105533389A (en) * | 2016-01-28 | 2016-05-04 | 广西大学 | Dendrobium officinale and carambola fruit solid drink particle and preparation method thereof |
| CN105535553A (en) * | 2016-03-10 | 2016-05-04 | 厦门塔斯曼生物工程有限公司 | Formula for regulating blood sugar of human body |
| CN106344808A (en) * | 2016-08-31 | 2017-01-25 | 浙江天方科技股份有限公司 | Compound dendrobium officinale health care product capable of assisting in reducing blood sugar |
| CN107997170A (en) * | 2017-11-30 | 2018-05-08 | 江苏康缘药业股份有限公司 | A kind of composition with hypolipemic function and preparation method thereof |
| CN107997170B (en) * | 2017-11-30 | 2021-04-20 | 江苏康缘药业股份有限公司 | A kind of composition with hypolipidemic function and preparation method thereof |
| CN108096474A (en) * | 2018-01-19 | 2018-06-01 | 郑州思邈堂生物科技有限公司 | Reduce by three high particles and preparation method thereof |
| CN114748582A (en) * | 2021-01-08 | 2022-07-15 | 遵义医科大学 | Traditional Chinese medicine composition for reducing blood sugar and application thereof |
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| Publication number | Publication date |
|---|---|
| CN104784505B (en) | 2018-02-16 |
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Effective date of registration: 20180802 Address after: 530228 the Guangxi Zhuang Autonomous Region Nanning Jiangnan District Suwei town agricultural and sideline products wholesale market Patentee after: Guangxi Nong Fengshan Dendrobium officinale Polytron Technologies Inc Address before: 530221 Nanning, Yinhai, the Guangxi Zhuang Autonomous Region, west of the Yinhai Road, 708-1 Shang Shang garden 3 rooms, 306 rooms. Patentee before: Dragon and phoenix mountain, Guangxi biotechnology Co., Ltd |