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CL2018003601A1 - A process for obtaining the functional allophycocyanin recombinant protein - Google Patents

A process for obtaining the functional allophycocyanin recombinant protein

Info

Publication number
CL2018003601A1
CL2018003601A1 CL2018003601A CL2018003601A CL2018003601A1 CL 2018003601 A1 CL2018003601 A1 CL 2018003601A1 CL 2018003601 A CL2018003601 A CL 2018003601A CL 2018003601 A CL2018003601 A CL 2018003601A CL 2018003601 A1 CL2018003601 A1 CL 2018003601A1
Authority
CL
Chile
Prior art keywords
variant
functional
allophycocyanin
subunit
obtaining
Prior art date
Application number
CL2018003601A
Other languages
Spanish (es)
Inventor
Balocchi Marta Bunster
Leone Jorge Dagnino
Oyanedel José Martínez
Roselló María Victoria Hinrich
López Diego Ortiz
Original Assignee
Univ Concepcion
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Concepcion filed Critical Univ Concepcion
Priority to CL2018003601A priority Critical patent/CL2018003601A1/en
Publication of CL2018003601A1 publication Critical patent/CL2018003601A1/en
Priority to PCT/CL2019/050125 priority patent/WO2020118463A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/405—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente tecnología corresponde a un proceso para la obtención de la proteína recombinante de Aloficocianina funcional, el cual está basado en el empleo de tres vectores bicistronicos que comprenden dos secuencias de interés cada uno, estas secuencias codifican para diferentes variantes de las subunidades necesarias para obtener una proteína funcional. Las variantes obtenidas corresponden a variante de la subunidad alfa de APC; una variante de la subunidad beta de APC; una variante de la subunidad S de la liasa heterodimérica; una variante de la subunidad U de la liasa heterodiméríca; una variante de la fícocianobilina óxido-reductasa; y una variante de hemooxigenasa.The present technology corresponds to a process for obtaining the functional allophycocyanin recombinant protein, which is based on the use of three bicistronic vectors comprising two sequences of interest each, these sequences encode different variants of the subunits necessary to obtain a functional protein The variants obtained correspond to a variant of the alpha subunit of APC; a variant of the beta subunit of APC; a variant of the S subunit of the heterodimeric lyase; a variant of the U subunit of the heterodimeric lyase; a variant of the phycocyanobilin oxide reductase; and a variant of hemoxygenase.

CL2018003601A 2018-12-14 2018-12-14 A process for obtaining the functional allophycocyanin recombinant protein CL2018003601A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CL2018003601A CL2018003601A1 (en) 2018-12-14 2018-12-14 A process for obtaining the functional allophycocyanin recombinant protein
PCT/CL2019/050125 WO2020118463A1 (en) 2018-12-14 2019-12-02 Method for producing the recombinant protein of functional allophycocyanin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CL2018003601A CL2018003601A1 (en) 2018-12-14 2018-12-14 A process for obtaining the functional allophycocyanin recombinant protein

Publications (1)

Publication Number Publication Date
CL2018003601A1 true CL2018003601A1 (en) 2019-05-17

Family

ID=66682762

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2018003601A CL2018003601A1 (en) 2018-12-14 2018-12-14 A process for obtaining the functional allophycocyanin recombinant protein

Country Status (2)

Country Link
CL (1) CL2018003601A1 (en)
WO (1) WO2020118463A1 (en)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102094029B (en) * 2009-12-11 2012-11-21 中国科学院海洋研究所 Method for preparing allophycocyanin tripolymer fluorescent protein
US20130171677A1 (en) * 2010-09-08 2013-07-04 Board Of Supervisors Of The University Of Louisiana System Recombinant phycobiliproteins with enhanced fluorescence and photochemical properties

Also Published As

Publication number Publication date
WO2020118463A1 (en) 2020-06-18

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