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CA3138949A1 - Systeme et procede de recuperation de materiau cible a partir de micropuits - Google Patents

Systeme et procede de recuperation de materiau cible a partir de micropuits Download PDF

Info

Publication number
CA3138949A1
CA3138949A1 CA3138949A CA3138949A CA3138949A1 CA 3138949 A1 CA3138949 A1 CA 3138949A1 CA 3138949 A CA3138949 A CA 3138949A CA 3138949 A CA3138949 A CA 3138949A CA 3138949 A1 CA3138949 A1 CA 3138949A1
Authority
CA
Canada
Prior art keywords
adaptor
region
target material
magnet
capture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3138949A
Other languages
English (en)
Inventor
Kalyan Handique
Swati Ranade
Vishal Sharma
Austin Payne
Sam Tuck
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio Rad Laboratories Inc
Original Assignee
Bio Rad Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US16/867,235 external-priority patent/US10900032B2/en
Application filed by Bio Rad Laboratories Inc filed Critical Bio Rad Laboratories Inc
Publication of CA3138949A1 publication Critical patent/CA3138949A1/fr
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • B01L9/527—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for microfluidic devices, e.g. used for lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1048—General features of the devices using the transfer device for another function
    • G01N2035/1053—General features of the devices using the transfer device for another function for separating part of the liquid, e.g. filters, extraction phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • General Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Fluid Mechanics (AREA)
  • Hematology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un système et un procédé de récupération et de traitement de matériau cible, le système comprenant : un adaptateur conçu pour s'interfacer avec une région de capture d'un substrat de capture pour capturer des particules dans un format de particule unique à l'intérieur d'un ensemble de puits, l'adaptateur comprenant une première région conçue pour s'interfacer avec la région de capture, une seconde région, et une cavité s'étendant de la première région à la seconde région ; et une structure de support reliée à l'adaptateur et fournissant un ensemble de modes de fonctionnement pour le déplacement de l'adaptateur par rapport au substrat de capture. Le système active des procédés magnétiques et/ou d'autres procédés de récupération de matériau cible basés sur la force (par exemple, dérivés de cellules uniques).
CA3138949A 2019-06-26 2020-06-19 Systeme et procede de recuperation de materiau cible a partir de micropuits Pending CA3138949A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962866726P 2019-06-26 2019-06-26
US62/866,726 2019-06-26
US16/867,235 US10900032B2 (en) 2019-05-07 2020-05-05 System and method for automated single cell processing
US16/867,235 2020-05-05
PCT/US2020/038647 WO2020263700A1 (fr) 2019-06-26 2020-06-19 Système et procédé de récupération de matériau cible à partir de micropuits

Publications (1)

Publication Number Publication Date
CA3138949A1 true CA3138949A1 (fr) 2020-12-30

Family

ID=74062083

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3138949A Pending CA3138949A1 (fr) 2019-06-26 2020-06-19 Systeme et procede de recuperation de materiau cible a partir de micropuits

Country Status (8)

Country Link
EP (1) EP3990926A4 (fr)
JP (1) JP2022539739A (fr)
KR (1) KR20220024734A (fr)
CN (1) CN113924494A (fr)
AU (1) AU2020304355B2 (fr)
CA (1) CA3138949A1 (fr)
SG (1) SG11202112150RA (fr)
WO (1) WO2020263700A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20250271415A1 (en) * 2022-03-29 2025-08-28 Sony Group Corporation Bioparticle analysis system, information processing device, and bioparticle analysis method
KR102785997B1 (ko) * 2022-12-27 2025-03-26 주식회사 아이노클 단일세포 시료 전처리 장치

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI102906B1 (fi) * 1998-02-23 1999-03-15 Bio Nobile Oy Menetelmä ja väline aineen siirtämiseksi
US20050106667A1 (en) * 2003-08-01 2005-05-19 Genentech, Inc Binding polypeptides with restricted diversity sequences
CN101370622B (zh) * 2006-01-18 2013-07-31 阿戈斯治疗公司 用于处理封闭容器中的样品的系统和方法以及相关装置
US8071395B2 (en) * 2007-12-12 2011-12-06 The Board Of Trustees Of The Leland Stanford Junior University Methods and apparatus for magnetic separation of cells
AU2010232972B2 (en) * 2009-04-01 2016-10-27 Dxterity Diagnostics Incorporated Chemical ligation dependent probe amplification (CLPA)
JP5292267B2 (ja) * 2009-12-16 2013-09-18 株式会社日立ハイテクノロジーズ 試料処理装置、試料処理方法及びこれらに使用する反応容器
JP2011234671A (ja) * 2010-05-11 2011-11-24 Hitachi High-Technologies Corp 核酸抽出装置
JP5802205B2 (ja) * 2010-06-22 2015-10-28 ユニバーサル・バイオ・リサーチ株式会社 生体関連物質の捕捉装置および生体関連物質の採取システム
US9174216B2 (en) * 2013-03-13 2015-11-03 DeNovo Science, Inc. System for capturing and analyzing cells
CN104254595A (zh) * 2012-02-13 2014-12-31 纽莫德克斯莫勒库拉尔公司 用于处理和检测核酸的微流体盒
EP2871460B1 (fr) * 2012-07-06 2016-09-21 Hitachi High-Technologies Corporation Kit d'extraction d'acides nucléiques, et extracteur d'acides nucléiques
US9707562B2 (en) * 2013-03-13 2017-07-18 Denovo Sciences, Inc. System for capturing and analyzing cells
US9518903B2 (en) * 2015-01-13 2016-12-13 Gilson, Inc. Adapter for sliding magnetic particle separation
CA3006985C (fr) * 2015-12-01 2021-11-09 Illumina, Inc. Systeme microfluidique numerique pour l'isolement de cellules uniques et la caracterisation d'analytes
AU2018323449B2 (en) * 2017-08-29 2020-09-03 Bio-Rad Laboratories, Inc. System and method for isolating and analyzing cells

Also Published As

Publication number Publication date
WO2020263700A1 (fr) 2020-12-30
SG11202112150RA (en) 2021-12-30
KR20220024734A (ko) 2022-03-03
CN113924494A (zh) 2022-01-11
AU2020304355B2 (en) 2023-04-06
EP3990926A4 (fr) 2023-09-13
AU2020304355A1 (en) 2021-11-25
EP3990926A1 (fr) 2022-05-04
JP2022539739A (ja) 2022-09-13

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