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CA3134848A1 - Structures polymeres tridimensionnelles sur cuves a circulation - Google Patents

Structures polymeres tridimensionnelles sur cuves a circulation Download PDF

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Publication number
CA3134848A1
CA3134848A1 CA3134848A CA3134848A CA3134848A1 CA 3134848 A1 CA3134848 A1 CA 3134848A1 CA 3134848 A CA3134848 A CA 3134848A CA 3134848 A CA3134848 A CA 3134848A CA 3134848 A1 CA3134848 A1 CA 3134848A1
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CA
Canada
Prior art keywords
flow cell
hydrogel
sequencing
dimensional
channel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3134848A
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English (en)
Inventor
Tarun Kumar Khurana
Elisabet ROSAS-CANYELLES
Yir-Shyuan WU
Hayden BLACK
Mathieu LESSARD-VIGER
Max ZIMMERLEY
Sean Ramirez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Inc
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from NL2024527A external-priority patent/NL2024527B1/en
Priority claimed from NL2024528A external-priority patent/NL2024528B1/en
Priority claimed from NL2024596A external-priority patent/NL2024596B1/en
Application filed by Illumina Inc filed Critical Illumina Inc
Priority claimed from PCT/US2020/062163 external-priority patent/WO2021108499A1/fr
Publication of CA3134848A1 publication Critical patent/CA3134848A1/fr
Pending legal-status Critical Current

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    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
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  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Fluid Mechanics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé de fabrication de structures polymères tridimensionnelles sur cuves à circulation qui consiste à charger une solution de précurseur de polymère sur une cuve à circulation. La solution de précurseur de polymère comprend un monomère, un agent de réticulation et un photo-initiateur. La cuve à circulation comprend au moins un canal destiné à recevoir la solution de précurseur de polymère. Ledit canal présente une surface intérieure supérieure et une surface intérieure inférieure. Le procédé consiste en outre à éclairer la solution de précurseur de polymère à travers un photomasque à motifs à l'aide d'une lumière à une longueur d'onde suffisante pour activer le photo-initiateur. L'activation du photo-initiateur polymérise au moins une partie de la solution de précurseur de polymère au-dessous des ouvertures dans le photomasque à motifs et forme des structures polymères tridimensionnelles qui s'étendent de la surface intérieure supérieure à la surface intérieure inférieure dudit canal.
CA3134848A 2019-11-27 2020-11-25 Structures polymeres tridimensionnelles sur cuves a circulation Pending CA3134848A1 (fr)

Applications Claiming Priority (13)

Application Number Priority Date Filing Date Title
US201962941197P 2019-11-27 2019-11-27
US201962941215P 2019-11-27 2019-11-27
US201962941242P 2019-11-27 2019-11-27
US62/941,215 2019-11-27
US62/941,242 2019-11-27
US62/941,197 2019-11-27
NL2024527A NL2024527B1 (en) 2019-11-27 2019-12-20 On-flow cell three-dimensional polymer structures
NL2024528 2019-12-20
NL2024528A NL2024528B1 (en) 2019-11-27 2019-12-20 On-flow cell three-dimensional polymer structures having functionalized surfaces
NL2024527 2019-12-20
NL2024596 2019-12-31
NL2024596A NL2024596B1 (en) 2019-11-27 2019-12-31 On-flow cell three-dimensional sequencing matrices
PCT/US2020/062163 WO2021108499A1 (fr) 2019-11-27 2020-11-25 Structures polymères tridimensionnelles sur cuves à circulation

Publications (1)

Publication Number Publication Date
CA3134848A1 true CA3134848A1 (fr) 2021-06-03

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Application Number Title Priority Date Filing Date
CA3134848A Pending CA3134848A1 (fr) 2019-11-27 2020-11-25 Structures polymeres tridimensionnelles sur cuves a circulation

Country Status (5)

Country Link
EP (1) EP3930888A1 (fr)
CN (1) CN113710364A (fr)
AU (1) AU2020391457A1 (fr)
CA (1) CA3134848A1 (fr)
IL (1) IL286667A (fr)

Cited By (2)

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FR3130844A1 (fr) * 2021-12-22 2023-06-23 Bforcure Composition et procédé d’amplification de séquences d’acides nucléiques
WO2025072571A1 (fr) * 2023-09-27 2025-04-03 Cellanome, Inc. Culture cellulaire dans des structures microfluidiques

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CN114316132B (zh) * 2022-01-28 2023-08-01 赛纳生物科技(北京)有限公司 一种乳液聚合合成功能性聚合物微球的方法
AU2023240153A1 (en) * 2022-03-22 2024-01-18 Illumina, Inc. Chemical planar array
EP4590730A1 (fr) * 2022-09-19 2025-07-30 Illumina, Inc. Particules de nanogel ayant une double fonctionnalité et une réactivité à la température pour le regroupement de particules dans des systèmes de séquençage d'acide nucléique

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AU2003205241A1 (en) * 2002-01-22 2003-09-02 The Penn State Research Foundation Drug candidate screening systems based on micropatterned hydrogels and microfluidic systems
US7713736B2 (en) * 2004-11-24 2010-05-11 Wisconsin Alumni Research Foundation Cell mimic platform and method
JP6464141B2 (ja) * 2013-03-14 2019-02-06 ライフ テクノロジーズ コーポレーション マトリックスアレイ及びそれを製造するための方法
US9937495B2 (en) * 2013-10-28 2018-04-10 Massachusetts Institute Of Technology Hydrogel microstructures with immiscible fluid isolation for small reaction volumes
US20200292944A1 (en) * 2015-11-18 2020-09-17 Massachusetts Institute Of Technology Method of making a patterned hydrogel and kit to make it
NL2019044B1 (en) * 2017-05-11 2018-11-15 Illumina Inc Protective surface coatings for flow cells
FI3662083T3 (fi) * 2017-08-01 2024-11-20 Illumina Inc Geneettisen materiaalin ja kirjastovalmisteen spatiaalinen indeksointi hydrogeelihelmiä ja virtauskennoja käyttämällä

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3130844A1 (fr) * 2021-12-22 2023-06-23 Bforcure Composition et procédé d’amplification de séquences d’acides nucléiques
WO2023118693A1 (fr) * 2021-12-22 2023-06-29 Bforcure Composition et procede d'amplification de sequences d'acides nucleiques
WO2025072571A1 (fr) * 2023-09-27 2025-04-03 Cellanome, Inc. Culture cellulaire dans des structures microfluidiques

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