CA3198478A1 - Variants de synthase de l'acide tetrahydrocannabinolique (thca), production et utilisation de ceux-ci - Google Patents
Variants de synthase de l'acide tetrahydrocannabinolique (thca), production et utilisation de ceux-ciInfo
- Publication number
- CA3198478A1 CA3198478A1 CA3198478A CA3198478A CA3198478A1 CA 3198478 A1 CA3198478 A1 CA 3198478A1 CA 3198478 A CA3198478 A CA 3198478A CA 3198478 A CA3198478 A CA 3198478A CA 3198478 A1 CA3198478 A1 CA 3198478A1
- Authority
- CA
- Canada
- Prior art keywords
- ggt
- seq
- cat
- gct
- tat
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
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- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y121/00—Oxidoreductases acting on X-H and Y-H to form an X-Y bond (1.21)
- C12Y121/03—Oxidoreductases acting on X-H and Y-H to form an X-Y bond (1.21) with oxygen as acceptor (1.21.3)
- C12Y121/03007—Tetrahydrocannabinolic acid synthase (1.21.3.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
L'invention concerne un vecteur recombinant qui comprend un promoteur, une étiquette de sécrétion, un gène d'intérêt (GOI) et éventuellement une étiquette de purification et/ou de détection. Le GOI comprend une séquence polynucléotidique telle que définie dans l'une quelconque des SEQ ID NO : 2 à 57 ou une séquence polynucléotidique ayant au moins 90 %, 95 %, 98 %, 99 % ou 99,9 % d'identité avec l'une quelconque des SEQ ID NO : 2 à 57. L'invention concerne également les séquences de polypeptides qui résultent de l'expression d'un GOI par un système d'expression hôte et un procédé de production de composés cannabinoïdes.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063113240P | 2020-11-13 | 2020-11-13 | |
| US63/113,240 | 2020-11-13 | ||
| PCT/US2021/058944 WO2022103938A1 (fr) | 2020-11-13 | 2021-11-11 | Variants de synthase de l'acide tétrahydrocannabinolique (thca), production et utilisation de ceux-ci |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3198478A1 true CA3198478A1 (fr) | 2022-05-19 |
Family
ID=81601664
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3198478A Pending CA3198478A1 (fr) | 2020-11-13 | 2021-11-11 | Variants de synthase de l'acide tetrahydrocannabinolique (thca), production et utilisation de ceux-ci |
Country Status (2)
| Country | Link |
|---|---|
| CA (1) | CA3198478A1 (fr) |
| WO (1) | WO2022103938A1 (fr) |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5469786B2 (ja) * | 2006-12-29 | 2014-04-16 | ライフセンサーズ、インク． | タンパク質の発現増強および精製の方法および組成 |
| CN102007214A (zh) * | 2008-02-20 | 2011-04-06 | 塞瑞斯公司 | 赋予植物提高的氮利用效率特性的核苷酸序列和相应的多肽 |
| JP6622793B2 (ja) * | 2014-08-25 | 2019-12-18 | ティーウィノット テクノロジーズ リミテッド | カンナビノイド化合物を同時作製する装置及び方法 |
| CA3019890A1 (fr) * | 2016-04-04 | 2017-10-12 | Teewinot Technologies Limited | Synthese bio-enzymatique de tetrahydrocannabivarine (thc-v), de cannabinol (cbn) et de cannabivarine (cbv) et leur utilisation en tant qu'agents therapeutiques |
| AU2018321260A1 (en) * | 2017-08-25 | 2020-03-05 | Y. Joseph Mo | Compositions and methods of delivery of pharmacological agents |
-
2021
- 2021-11-11 WO PCT/US2021/058944 patent/WO2022103938A1/fr not_active Ceased
- 2021-11-11 CA CA3198478A patent/CA3198478A1/fr active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| WO2022103938A1 (fr) | 2022-05-19 |
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