[go: up one dir, main page]

CA3015204A1 - Procedes et systemes pour la detection d'acides nucleiques cibles - Google Patents

Procedes et systemes pour la detection d'acides nucleiques cibles Download PDF

Info

Publication number
CA3015204A1
CA3015204A1 CA3015204A CA3015204A CA3015204A1 CA 3015204 A1 CA3015204 A1 CA 3015204A1 CA 3015204 A CA3015204 A CA 3015204A CA 3015204 A CA3015204 A CA 3015204A CA 3015204 A1 CA3015204 A1 CA 3015204A1
Authority
CA
Canada
Prior art keywords
probe
chromosome
target
nucleic acid
specific
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA3015204A
Other languages
English (en)
Inventor
Vladimir I. Bashkirov
Theofilos Kotseroglou
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA3015204A1 publication Critical patent/CA3015204A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/112—Disease subtyping, staging or classification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/118—Prognosis of disease development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)

Abstract

La présente invention concerne des procédés et des systèmes pour la détection et l'identification d'acides nucléiques.
CA3015204A 2016-02-20 2017-02-20 Procedes et systemes pour la detection d'acides nucleiques cibles Abandoned CA3015204A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201662297826P 2016-02-20 2016-02-20
US62/297,826 2016-02-20
US201662300623P 2016-02-26 2016-02-26
US62/300,623 2016-02-26
PCT/US2017/018569 WO2017143317A1 (fr) 2016-02-20 2017-02-20 Procédés et systèmes pour la détection d'acides nucléiques cibles

Publications (1)

Publication Number Publication Date
CA3015204A1 true CA3015204A1 (fr) 2017-08-24

Family

ID=59625499

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3015204A Abandoned CA3015204A1 (fr) 2016-02-20 2017-02-20 Procedes et systemes pour la detection d'acides nucleiques cibles

Country Status (7)

Country Link
US (2) US20180030519A1 (fr)
EP (1) EP3417059A4 (fr)
JP (1) JP2019512101A (fr)
CN (1) CN109072225A (fr)
AU (1) AU2017221492A1 (fr)
CA (1) CA3015204A1 (fr)
WO (1) WO2017143317A1 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019075091A1 (fr) * 2017-10-11 2019-04-18 Expansion Technologies Hybridation in situ multiplexée de sections de tissu pour transcriptomique à résolution spatiale avec microscopie à expansion
WO2019103996A1 (fr) 2017-11-21 2019-05-31 Expansion Technologies Hybridation in situ compatible et multiplexée par microscopie à expansion de sections tissulaires enrobées dans de la paraffine et fixées dans la formaline en vue d'une transcriptomique à résolution spatiale
EP4647508A2 (fr) 2018-04-02 2025-11-12 Enumera Molecular, Inc. Procédés, systèmes et compositions pour compter des molécules d'acide nucléique
EP3947718A4 (fr) 2019-04-02 2022-12-21 Enumera Molecular, Inc. Procédés, systèmes et compositions de comptage de molécules d'acide nucléique
KR102261979B1 (ko) * 2019-10-28 2021-06-07 재단법인 대구경북첨단의료산업진흥재단 핵산분해효소 연쇄 반응
CN111088252B (zh) * 2019-12-30 2021-07-13 西安交通大学 一种dna上5-羟甲基尿嘧啶的特异性标记方法
WO2022032195A2 (fr) 2020-08-06 2022-02-10 Singular Genomics Systems, Inc. Séquençage spatial
EP4074839A1 (fr) * 2021-04-16 2022-10-19 Biotype GmbH Sonde tx oligonucléotidique optimisée pour une analyse de multiplexage d'acides nucléiques et méthode de multiplexage
CN113337579B (zh) * 2021-05-13 2022-08-12 厦门先能生物科技有限公司 一种检测样品中一种或多种靶核酸的存在或其水平的方法
CN113897418B (zh) * 2021-06-28 2023-08-22 华中科技大学 检测dna点突变的探针、试剂盒及应用
WO2023077109A2 (fr) * 2021-10-29 2023-05-04 Singular Genomics Systems, Inc. Sondes de partenaire d'oligonucléotide divisé
JP7748093B2 (ja) * 2021-11-10 2025-10-02 国立大学法人広島大学 鋳型ｄｎａの製造方法
WO2024249961A1 (fr) 2023-06-01 2024-12-05 Singular Genomics Systems, Inc. Procédés et sondes pour détecter des séquences polynucléotidiques dans des cellules et des tissus
WO2025205006A1 (fr) * 2024-03-29 2025-10-02 ソニーグループ株式会社 Procédé d'analyse, procédé de classification de l'état de la chromatine, procédé de dépistage du cancer, système d'analyse, système de dépistage du cancer ou de petites lésions résiduelles, et kit d'analyse

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6465193B2 (en) * 1998-12-11 2002-10-15 The Regents Of The University Of California Targeted molecular bar codes and methods for using the same
EP1497455A4 (fr) * 2001-11-09 2005-12-28 Aclara Biosciences Inc Detection de sequences d'acides nucleiques par clivage et separation de structures marquees
US20040121338A1 (en) * 2002-12-19 2004-06-24 Alsmadi Osama A. Real-time detection of rolling circle amplification products
US20110318740A1 (en) * 2005-12-09 2011-12-29 Life Technologies Corporation Reduced Interference from Single Strand Binding Proteins
US8551697B1 (en) * 2005-12-09 2013-10-08 Applied Biosystems, Llc Electrochemical polynucleotide detection comprising ligation
WO2007092538A2 (fr) * 2006-02-07 2007-08-16 President And Fellows Of Harvard College Procédés de confection de sondes nucléotidiques pour séquençage et synthèse
KR100864138B1 (ko) * 2007-09-04 2008-10-16 주식회사 노아바이오텍 정자의 고순도 성 분리방법
US8450063B2 (en) * 2009-01-28 2013-05-28 Fluidigm Corporation Determination of copy number differences by amplification
WO2010135310A1 (fr) * 2009-05-20 2010-11-25 Biosite Incorporated Glycosylase/lyase d'adn et substrats d'endonucléase ap
EP2521796B1 (fr) * 2010-01-04 2015-07-29 Life Technologies Corporation Procédés de séquençage d'adn et détecteurs et systèmes pour les mettre en uvre
AU2016243036B2 (en) * 2015-04-03 2022-02-17 Abbott Laboratories Devices and methods for sample analysis

Also Published As

Publication number Publication date
US20180030519A1 (en) 2018-02-01
US20210040539A1 (en) 2021-02-11
AU2017221492A1 (en) 2018-09-06
CN109072225A (zh) 2018-12-21
EP3417059A1 (fr) 2018-12-26
JP2019512101A (ja) 2019-05-09
WO2017143317A1 (fr) 2017-08-24
EP3417059A4 (fr) 2019-10-30

Similar Documents

Publication Publication Date Title
US20210040539A1 (en) Methods and systems for detecting target nucleic acids
AU2012251027B2 (en) Quantitative nuclease protection Assay (qNPA) and sequencing (qNPS) improvements
EP3967771B1 (fr) Sondes de discrimination et de multiplexage améliorés dans des analyses d'acide nucléique
US10081825B2 (en) Methods for amplification of nucleic acids utilizing a circularized template prepared from a target nucleic acid
US20090061424A1 (en) Universal ligation array for analyzing gene expression or genomic variations
EP2971144B1 (fr) Procédé d'amplification d'acides nucléiques sur un support solide
CN114250278A (zh) 捕获、检测和定量小rna的方法、组合物与试剂盒
EP2971124B1 (fr) Procédés pour l'amplification d'acides nucléiques à l'aide d'oligonucléotides clamp
WO2003012142A1 (fr) Detection d'acides nucleiques par acp en temps reel faisant intervenir des amorces chimeres arn-adn
KR20140123858A (ko) 폴리뉴클레오티드 및 그의 용도
US10590453B2 (en) Methods for amplification of nucleic acids utilizing a circularized template prepared from a target nucleic acid
CN105063190A (zh) 微小rna的固相芯片恒温检测方法
CN108026569B (zh) 用于催化性测定的方法和组合物
WO2016077602A1 (fr) Méthodes de séquençage de nouvelle génération
AU2015203545A1 (en) Quantitative nuclease protection assay (qnpa) and sequencing (qnps) improvements
HK40070845A (en) Probes for improved melt discrimination and multiplexing in nucleic acid assays
HK1254039B (zh) 用於催化性测定的方法和组合物
US20160122810A1 (en) Systems and methods for nucleic acid capture
KR20140073214A (ko) 표적 핵산을 증폭 또는 분석하는 방법 및 표적 핵산을 증폭하기 위한 키트 또는 조성물

Legal Events

Date Code Title Description
FZDE Discontinued

Effective date: 20220823

FZDE Discontinued

Effective date: 20220823