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CA3093451A1 - Analyse de pathogenes multiplex sur micropuce et ses utilisations - Google Patents

Analyse de pathogenes multiplex sur micropuce et ses utilisations Download PDF

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Publication number
CA3093451A1
CA3093451A1 CA3093451A CA3093451A CA3093451A1 CA 3093451 A1 CA3093451 A1 CA 3093451A1 CA 3093451 A CA3093451 A CA 3093451A CA 3093451 A CA3093451 A CA 3093451A CA 3093451 A1 CA3093451 A1 CA 3093451A1
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CA
Canada
Prior art keywords
seq
dna
specific
nos
amplicons
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3093451A
Other languages
English (en)
Inventor
Michael Edward Hogan
Melissa Rose May
Frederick Henry Eggers
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
PathogenDx Inc
Original Assignee
PathogenDx Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US15/916,062 external-priority patent/US11542498B2/en
Priority claimed from US15/916,036 external-priority patent/US10612075B2/en
Priority claimed from US16/158,276 external-priority patent/US10513745B2/en
Application filed by PathogenDx Inc filed Critical PathogenDx Inc
Publication of CA3093451A1 publication Critical patent/CA3093451A1/fr
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention concerne un système de micropuce à réseau tridimensionnel pour la détection et l'analyse de séquences d'ADN et des kits comprenant des composants de celui-ci. Dans le système, un support solide comporte une pluralité de lieurs polymères bifonctionnels, éventuellement dotés d'une étiquette fluorescente, ayant chacun un domaine supérieur et une extrémité inférieure jointe à celui-ci au niveau de ladite l'extrémité inférieure et une pluralité de sondes d'acide nucléique jointes au domaine supérieur de la pluralité de lieurs polymères bifonctionnels. Un procédé de fabrication du système de micropuce et des procédés pour détecter et identifier des pathogènes dans un échantillon végétal ou pour déterminer le nombre de copies d'un ADN pathogène dans un échantillon d'acide nucléique non purifié à l'aide d'un nombre de copies connu d'ADN synthétique à titre d'étalon interne sont en outre décrits.
CA3093451A 2018-03-08 2019-03-08 Analyse de pathogenes multiplex sur micropuce et ses utilisations Pending CA3093451A1 (fr)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US15/916,036 2018-03-08
US15/916,062 US11542498B2 (en) 2015-12-28 2018-03-08 Microarray based multiplex pathogen analysis and uses thereof
US15/916,036 US10612075B2 (en) 2015-12-28 2018-03-08 Microarray based multiplex pathogen analysis and uses thereof
US15/916,062 2018-03-08
US16/158,276 2018-10-11
US16/158,276 US10513745B2 (en) 2015-12-28 2018-10-11 Microarray based multiplex pathogen analysis and uses thereof
PCT/US2019/021317 WO2019173695A2 (fr) 2018-03-08 2019-03-08 Analyse de pathogènes multiplex sur micropuce et ses utilisations

Publications (1)

Publication Number Publication Date
CA3093451A1 true CA3093451A1 (fr) 2019-09-12

Family

ID=67847534

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3093451A Pending CA3093451A1 (fr) 2018-03-08 2019-03-08 Analyse de pathogenes multiplex sur micropuce et ses utilisations

Country Status (9)

Country Link
EP (1) EP3762508A4 (fr)
JP (1) JP2021516995A (fr)
KR (1) KR20210028142A (fr)
CN (1) CN112154215A (fr)
AU (1) AU2019231794A1 (fr)
CA (1) CA3093451A1 (fr)
MX (1) MX2020009282A (fr)
WO (1) WO2019173695A2 (fr)
ZA (1) ZA202006213B (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20210317540A1 (en) * 2015-12-28 2021-10-14 Pathogendx, Inc. Microarray-Based Multiplex Fungal Pathogen Analysis
WO2022033453A1 (fr) * 2020-08-12 2022-02-17 深圳市真迈生物科技有限公司 Puce modifiée par des amines, son procède de préparation et son utilisation
WO2023027073A1 (fr) * 2021-08-27 2023-03-02 株式会社島津製作所 Procédé de pcr quantitative utilisant une commande interne

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE68928853T2 (de) * 1988-05-20 1999-08-05 Cetus Corp., Emeryville, Calif. Befestigung von sequenzspezifischen proben
AU746737B2 (en) * 1996-11-06 2002-05-02 Sequenom, Inc. Compositions and methods for immobilizing nucleic acids to solid supports
JP2001511361A (ja) * 1997-07-22 2001-08-14 ラピジーン，インコーポレイテッド 核酸アレイ上で行われる増幅および他の酵素反応
US6238869B1 (en) * 1997-12-19 2001-05-29 High Throughput Genomics, Inc. High throughput assay system
EP1112379A4 (fr) * 1998-08-14 2004-12-15 Univ California NOUVEL AMPLICON DE LA REGION 20q13 DU CHROMOSOME HUMAIN 20 ET UTILISATIONS DE CELUI-CI
AU2002318306B2 (en) * 2001-07-11 2008-06-19 Baylor College Of Medicine Methods and devices based upon a novel form of nucleic acid duplex on a surface
AU2002366046A1 (en) * 2001-10-19 2003-06-10 Proligo Llc Nucleic acid probes and methods to detect and/or quantify nucleic acid analytes
CA2604095A1 (fr) * 2005-04-12 2006-10-19 454 Life Sciences Corporation Procedes de determination de variantes de sequence utilisant un sequencage des amplicons
JP5196846B2 (ja) * 2007-05-14 2013-05-15 キヤノン株式会社 プローブセット、プローブ担体、検査方法及びｄｎａ検出用キット
US8691509B2 (en) * 2009-04-02 2014-04-08 Fluidigm Corporation Multi-primer amplification method for barcoding of target nucleic acids
EP2532754A1 (fr) * 2011-06-07 2012-12-12 Koninklijke Philips Electronics N.V. Dispositifs et procédés pour la capture efficace d'acides nucléiques
WO2014185960A2 (fr) * 2013-05-14 2014-11-20 Genomics Usa, Inc. Composition et procédés pour piéger une protéine sur une surface
KR20180008785A (ko) * 2015-06-17 2018-01-24 도요세이칸 그룹 홀딩스 가부시키가이샤 미생물의 검사 방법, 미생물의 검사 키트, 미생물 검사용 마이크로어레이, 곰팡이 검출용 담체, 곰팡이의 검출 방법 및 곰팡이 검출용 키트

Also Published As

Publication number Publication date
MX2020009282A (es) 2021-01-08
ZA202006213B (en) 2022-01-26
EP3762508A4 (fr) 2021-12-15
AU2019231794A1 (en) 2020-10-29
WO2019173695A2 (fr) 2019-09-12
WO2019173695A3 (fr) 2019-12-26
JP2021516995A (ja) 2021-07-15
KR20210028142A (ko) 2021-03-11
CN112154215A (zh) 2020-12-29
EP3762508A2 (fr) 2021-01-13

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