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CA3081320A1 - Edition de genes de cellules primitives - Google Patents

Edition de genes de cellules primitives Download PDF

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Publication number
CA3081320A1
CA3081320A1 CA3081320A CA3081320A CA3081320A1 CA 3081320 A1 CA3081320 A1 CA 3081320A1 CA 3081320 A CA3081320 A CA 3081320A CA 3081320 A CA3081320 A CA 3081320A CA 3081320 A1 CA3081320 A1 CA 3081320A1
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CA
Canada
Prior art keywords
tcr
cell
nucleotide sequence
cells
nucleotide
Prior art date
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Pending
Application number
CA3081320A
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English (en)
Inventor
Kyle Martin Jacoby
Alexis FRANZUSOFF
Stefanie MANDL-CASHMAN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Adoc SSF LLC
Original Assignee
Pact Pharma Inc
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Filing date
Publication date
Application filed by Pact Pharma Inc filed Critical Pact Pharma Inc
Publication of CA3081320A1 publication Critical patent/CA3081320A1/fr
Pending legal-status Critical Current

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14—Blood; Artificial blood
    • A61K35/17—Lymphocytes; B-cells; T-cells; Natural killer cells; Interferon-activated or cytokine-activated lymphocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/10—Cellular immunotherapy characterised by the cell type used
    • A61K40/11—T-cells, e.g. tumour infiltrating lymphocytes [TIL] or regulatory T [Treg] cells; Lymphokine-activated killer [LAK] cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/30—Cellular immunotherapy characterised by the recombinant expression of specific molecules in the cells of the immune system
    • A61K40/32—T-cell receptors [TCR]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A61K40/41—Vertebrate antigens
    • A61K40/42—Cancer antigens
    • A61K40/4201—Neoantigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A61K40/41—Vertebrate antigens
    • A61K40/42—Cancer antigens
    • A61K40/4271—Melanoma antigens
    • A61K40/4272—Melan-A/MART
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/62—DNA sequences coding for fusion proteins
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0646—Natural killers cells [NK], NKT cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K40/00
    • A61K2239/38—Indexing codes associated with cellular immunotherapy of group A61K40/00 characterised by the dose, timing or administration schedule
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K40/00
    • A61K2239/46—Indexing codes associated with cellular immunotherapy of group A61K40/00 characterised by the cancer treated
    • A61K2239/48—Blood cells, e.g. leukemia or lymphoma
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
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    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2310/00—Structure or type of the nucleic acid
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  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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Abstract

La présente invention concerne des méthodes et des compositions pour l'édition de gènes de cellules primitives à médiation par des nucléases sans l'utilisation d'une administration à médiation virale. L'invention concerne également des méthodes de traitement faisant appel aux cellules primitives dont le génome a été édité.
CA3081320A 2017-10-30 2018-10-30 Edition de genes de cellules primitives Pending CA3081320A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201762579113P 2017-10-30 2017-10-30
US201762579114P 2017-10-30 2017-10-30
US62/579,113 2017-10-30
US62/579,114 2017-10-30
PCT/US2018/058230 WO2019089610A1 (fr) 2017-10-30 2018-10-30 Édition de gènes de cellules primitives

Publications (1)

Publication Number Publication Date
CA3081320A1 true CA3081320A1 (fr) 2019-05-09

Family

ID=66332290

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3081320A Pending CA3081320A1 (fr) 2017-10-30 2018-10-30 Edition de genes de cellules primitives

Country Status (13)

Country Link
US (7) US10584357B2 (fr)
EP (1) EP3703712A4 (fr)
JP (2) JP7397791B2 (fr)
CN (2) CN111918659B (fr)
AU (1) AU2018357861B2 (fr)
BR (1) BR112020008704A2 (fr)
CA (1) CA3081320A1 (fr)
IL (1) IL274351B2 (fr)
MX (1) MX2020004541A (fr)
RU (1) RU2020116585A (fr)
SG (1) SG11202004026QA (fr)
TW (1) TWI716758B (fr)
WO (1) WO2019089610A1 (fr)

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US20200362355A1 (en) 2017-06-15 2020-11-19 The Regents Of The University Of California Targeted non-viral dna insertions
IL274179B2 (en) 2017-10-27 2024-02-01 Univ California Targeted replacement of endogenous T cell receptors
BR112020008704A2 (pt) * 2017-10-30 2020-11-10 Pact Pharma, Inc. edição de genes de células primárias
KR20200138334A (ko) 2018-04-02 2020-12-09 팩트 파마, 인크. 펩티드-MHC comPACT
SG11202103571XA (en) * 2018-10-16 2021-05-28 Intellia Therapeutics Inc Compositions and methods for immunotherapy
SG11202111532SA (en) 2019-05-01 2021-11-29 Pact Pharma Inc Compositions and methods for the treatment of cancer using a cdb engineered t cell therapy
EP4010463A4 (fr) * 2019-08-05 2024-05-08 Cartherics Pty. Ltd. Cellules immunitaires exprimant des récepteurs cellulaires modifiés et leurs procédés de fabrication
WO2021041519A1 (fr) 2019-08-26 2021-03-04 Pact Pharma, Inc. Méthodes de réalisation de séquençage guide sur des lymphocytes t humains primaires
JP2022552819A (ja) * 2019-10-08 2022-12-20 パクト ファーマ インコーポレイテッド 遺伝子改変自己ｔ細胞免疫療法を使用する治療方法
JP2022552197A (ja) * 2019-10-10 2022-12-15 パクト ファーマ インコーポレイテッド 自己細胞療法で免疫療法の非応答者を治療する方法
EP4110356A4 (fr) * 2020-02-28 2024-03-27 Wisconsin Alumni Research Foundation Génération non virale de lymphocytes t récepteurs d'antigènes chimériques obtenus par édition génique
IL297539A (en) * 2020-04-24 2022-12-01 Pact Pharma Inc Methods for determining the efficiency of gene editing in cells
EP4165190A4 (fr) * 2020-06-12 2024-09-25 Adoc Ssf, Llc Compositions et procédés de fabrication de thérapies de lymphocytes t autologues
CA3191148A1 (fr) * 2020-08-28 2022-03-03 Aaron Cooper Cellules immunitaires modifiees avec des recepteurs d'amorcage
EP4196490A4 (fr) * 2020-10-27 2025-02-26 Adoc Ssf, Llc Compositions et méthodes pour le traitement du cancer au moyen d'une thérapie cellulaire nouvelle génération à base de lymphocytes t modifiés
AU2022245323A1 (en) * 2021-03-24 2023-09-28 Genentech, Inc. Efficient tcr gene editing in t lymphocytes
JP2024519895A (ja) * 2021-05-18 2024-05-21 賽斯尓▲チン▼生物技術（上海）有限公司 細胞を修飾する方法
MX2023014745A (es) 2021-06-11 2024-02-16 Pact Pharma Inc Metodos para valorar productos de celulas.
AU2024228388A1 (en) 2023-02-27 2025-10-02 Adaptive Biotechnologies Corp. Therapeutic t cell receptors targeting kras g12d

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US20200165634A1 (en) 2020-05-28
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JP7397791B2 (ja) 2023-12-13
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IL274351B2 (en) 2025-10-01
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CN111918659A (zh) 2020-11-10
BR112020008704A2 (pt) 2020-11-10
US20200181650A1 (en) 2020-06-11
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