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CA3054593A1 - Milieu de perfusion - Google Patents

Milieu de perfusion Download PDF

Info

Publication number
CA3054593A1
CA3054593A1 CA3054593A CA3054593A CA3054593A1 CA 3054593 A1 CA3054593 A1 CA 3054593A1 CA 3054593 A CA3054593 A CA 3054593A CA 3054593 A CA3054593 A CA 3054593A CA 3054593 A1 CA3054593 A1 CA 3054593A1
Authority
CA
Canada
Prior art keywords
cell
perfusion
potassium
medium
serum
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3054593A
Other languages
English (en)
Inventor
Samantha WANG
Henry Lin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim International GmbH
Original Assignee
Boehringer Ingelheim International GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim International GmbH filed Critical Boehringer Ingelheim International GmbH
Publication of CA3054593A1 publication Critical patent/CA3054593A1/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

L'invention concerne un procédé de mise en culture de cellules mammaliennes exprimant une protéine hétérologue dans une culture de cellules de perfusion, comprenant l'augmentation de la concentration de potassium et la réduction du rapport molaire du sodium au potassium pour réduire le prélèvement de cellules inutiles et augmenter la production de protéines. L'invention concerne en outre un milieu de perfusion sans sérum comprenant une forte concentration d'ions potassium et un faible rapport molaire sodium/potassium, et l'utilisation de ce milieu pour une utilisation dans la culture de cellules dans une culture de perfusion pendant la phase de production ou pour réduire le volume de purge de cellules pendant la phase de production.
CA3054593A 2017-03-31 2018-03-27 Milieu de perfusion Pending CA3054593A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762479422P 2017-03-31 2017-03-31
US62/479422 2017-03-31
PCT/EP2018/057757 WO2018178069A1 (fr) 2017-03-31 2018-03-27 Milieu de perfusion

Publications (1)

Publication Number Publication Date
CA3054593A1 true CA3054593A1 (fr) 2018-10-04

Family

ID=61911547

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3054593A Pending CA3054593A1 (fr) 2017-03-31 2018-03-27 Milieu de perfusion

Country Status (9)

Country Link
US (2) US11702628B2 (fr)
EP (1) EP3601586A1 (fr)
JP (2) JP6943972B2 (fr)
KR (1) KR102604988B1 (fr)
CN (1) CN110520538B (fr)
AU (1) AU2018241849B2 (fr)
CA (1) CA3054593A1 (fr)
SG (1) SG11201909087PA (fr)
WO (1) WO2018178069A1 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2015369809B2 (en) 2014-12-22 2022-03-17 Genzyme Corporation Methods of culturing a mammalian cell
WO2019229764A1 (fr) * 2018-06-01 2019-12-05 Gennova Biopharmaceuticals Limited Procédé de production de tnk-tpa recombinant par un système de perfusion à lit à garnissage
WO2020086408A1 (fr) * 2018-10-26 2020-04-30 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Bioprocédé d'expression génique transitoire basé sur une perfusion à haut rendement
US20220364034A1 (en) * 2018-11-02 2022-11-17 WuXi Biologics Ireland Limited Cell culture process by intensified perfusion with continuous harvest and without cell bleeding
WO2021058713A1 (fr) * 2019-09-27 2021-04-01 Boehringer Ingelheim International Gmbh Milieu de perfusion concentré
CN110760481B (zh) * 2019-10-30 2021-04-02 武汉科技大学 一种改良rpmi1640培养基及其应用
US20220403316A1 (en) * 2019-11-15 2022-12-22 Lonza Ltd Process and system for producing an inoculum
WO2021165302A1 (fr) * 2020-02-18 2021-08-26 Boehringer Ingelheim International Gmbh Procédés de culture de cellules de mammifère
CN115768879A (zh) 2020-04-28 2023-03-07 莱尔免疫制药公司 用于培养细胞的方法
CN111690057B (zh) * 2020-06-24 2021-04-23 哈尔滨元亨生物药业有限公司 一种重组犬细小病毒单克隆抗体生产方法
WO2022203051A1 (fr) * 2021-03-25 2022-09-29 株式会社カネカ Procédé de production d'une population de cellules souches pluripotentes
IL307715A (en) * 2021-04-14 2023-12-01 Genzyme Corp Perfusion methods for growing a mammalian cell culture
KR20240154572A (ko) 2022-02-21 2024-10-25 아지노모토 가부시키가이샤 관류 배양 방법
CN115651874B (zh) * 2022-11-21 2023-04-28 清华大学 一种培养嗜盐微生物的培养基和培养方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0787799B2 (ja) 1988-03-30 1995-09-27 森永製菓株式会社 モノクローナル抗体生産増強法
WO1997048808A1 (fr) * 1996-06-19 1997-12-24 Chiron Corporation Production bacterienne d'interferon-beta dans un milieu a faible concentration d'ions de sodium et de potassium
EP2500413A1 (fr) * 2006-09-13 2012-09-19 Abbott Laboratories Améliorations de culture cellulaire
ES2533074T3 (es) * 2010-04-26 2015-04-07 Novartis Ag Medio de cultivo celular mejorado
HUE033279T2 (en) * 2011-07-01 2017-11-28 Amgen Inc Mammalian cell culture
EP3227454B1 (fr) * 2014-12-01 2020-01-29 Amgen Inc. Procédé de manipulation du taux de contenu de glycane d'une glycoprotéine

Also Published As

Publication number Publication date
AU2018241849A1 (en) 2019-09-12
JP2021192628A (ja) 2021-12-23
US11702628B2 (en) 2023-07-18
CN110520538B (zh) 2024-02-20
SG11201909087PA (en) 2019-10-30
WO2018178069A1 (fr) 2018-10-04
KR20190130028A (ko) 2019-11-20
US20200377849A1 (en) 2020-12-03
JP6943972B2 (ja) 2021-10-06
US20230313125A1 (en) 2023-10-05
EP3601586A1 (fr) 2020-02-05
CN110520538A (zh) 2019-11-29
KR102604988B1 (ko) 2023-11-24
JP2020511978A (ja) 2020-04-23
AU2018241849B2 (en) 2023-06-22
US12371656B2 (en) 2025-07-29

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