CA2863635A1 - Systeme de reconnaissance et de quantification base sur des selecteurs et procede pour multiples analytes au cours d'une seule et meme analyse - Google Patents

Systeme de reconnaissance et de quantification base sur des selecteurs et procede pour multiples analytes au cours d'une seule et meme analyse Download PDF

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Publication number: CA2863635A1
Authority: CA; Canada
Prior art keywords: analytes; affinity; dimension; separation; column
Prior art date: 2012-02-02
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Abandoned

Application number

CA2863635A

Other languages

English (en)

Inventor

Fred E. Regnier

Nicholas B. Herold

Kevin W. Meyer

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Perfinity Biosciences Inc

Original Assignee

Perfinity Biosciences Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2012-02-02

Filing date

2013-01-30

Publication date

2013-08-08

2013-01-30 Application filed by Perfinity Biosciences Inc filed Critical Perfinity Biosciences Inc

2013-08-08 Publication of CA2863635A1 publication Critical patent/CA2863635A1/fr

Status Abandoned legal-status Critical Current

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229920001282 polysaccharide Polymers 0.000 description 1
239000005017 polysaccharide Substances 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
150000003141 primary amines Chemical class 0.000 description 1
230000006916 protein interaction Effects 0.000 description 1
229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
239000011541 reaction mixture Substances 0.000 description 1
238000006722 reduction reaction Methods 0.000 description 1
230000004044 response Effects 0.000 description 1
230000000717 retained effect Effects 0.000 description 1
230000007017 scission Effects 0.000 description 1
238000002098 selective ion monitoring Methods 0.000 description 1
239000012279 sodium borohydride Substances 0.000 description 1
229910000033 sodium borohydride Inorganic materials 0.000 description 1
BEOOHQFXGBMRKU-UHFFFAOYSA-N sodium cyanoborohydride Chemical compound [Na+].[B-]C#N BEOOHQFXGBMRKU-UHFFFAOYSA-N 0.000 description 1
230000003595 spectral effect Effects 0.000 description 1
238000001228 spectrum Methods 0.000 description 1
238000010183 spectrum analysis Methods 0.000 description 1
239000008117 stearic acid Substances 0.000 description 1
230000001629 suppression Effects 0.000 description 1
238000002198 surface plasmon resonance spectroscopy Methods 0.000 description 1
238000004885 tandem mass spectrometry Methods 0.000 description 1
238000012360 testing method Methods 0.000 description 1
229960002175 thyroglobulin Drugs 0.000 description 1
230000007704 transition Effects 0.000 description 1
YUYCVXFAYWRXLS-UHFFFAOYSA-N trimethoxysilane Chemical compound CO[SiH](OC)OC YUYCVXFAYWRXLS-UHFFFAOYSA-N 0.000 description 1
230000004304 visual acuity Effects 0.000 description 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase

Landscapes

Health & Medical Sciences (AREA)
Immunology (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Biomedical Technology (AREA)
Chemical & Material Sciences (AREA)
Hematology (AREA)
Urology & Nephrology (AREA)
Food Science & Technology (AREA)
General Physics & Mathematics (AREA)
Cell Biology (AREA)
Biotechnology (AREA)
Medicinal Chemistry (AREA)
Physics & Mathematics (AREA)
Analytical Chemistry (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Microbiology (AREA)
Pathology (AREA)
Peptides Or Proteins (AREA)
Investigating Or Analysing Biological Materials (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Treatment Of Liquids With Adsorbents In General (AREA)
Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Solid-Sorbent Or Filter-Aiding Compositions (AREA)

CA2863635A 2012-02-02 2013-01-30 Systeme de reconnaissance et de quantification base sur des selecteurs et procede pour multiples analytes au cours d'une seule et meme analyse Abandoned CA2863635A1 (fr)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US201261594193P	2012-02-02	2012-02-02
US61/594,193		2012-02-02
PCT/US2013/023727 WO2013116260A1 (fr)	2012-02-02	2013-01-30	Système de reconnaissance et de quantification basé sur des sélecteurs et procédé pour multiples analytes au cours d'une seule et même analyse

Publications (1)

Publication Number	Publication Date
CA2863635A1 true CA2863635A1 (fr)	2013-08-08

Family

ID=48905766

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA2863635A Abandoned CA2863635A1 (fr)	2012-02-02	2013-01-30	Systeme de reconnaissance et de quantification base sur des selecteurs et procede pour multiples analytes au cours d'une seule et meme analyse

Country Status (12)

Country	Link
EP (1)	EP2820425A1 (fr)
JP (1)	JP2015505619A (fr)
KR (1)	KR20140137353A (fr)
CN (1)	CN104160278A (fr)
AU (1)	AU2013215305A1 (fr)
BR (1)	BR112014019134A8 (fr)
CA (1)	CA2863635A1 (fr)
IL (1)	IL233897A0 (fr)
IN (1)	IN2014DN06899A (fr)
RU (1)	RU2014135576A (fr)
SG (1)	SG11201404526PA (fr)
WO (1)	WO2013116260A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO2015084748A1 (fr) *	2013-12-03	2015-06-11	Perfinity Biosciences, Inc	Réacteur unique pour simplifier des flux de travaux de préparation d'échantillons
WO2016118489A1 (fr) *	2015-01-19	2016-07-28	Siscapa Assay Technologies, Inc.	Analyse combinée de petites molécules et de protéines par spectrométrie de masse
CN119881293A (zh) *	2018-03-21	2025-04-25	沃特世科技公司	基于非抗体高亲和力的样品制备、吸附剂、装置和方法
AU2019264349B2 (en) *	2018-04-30	2025-01-16	Phynexus Inc.	Qualitative analysis of proteins
WO2019229058A1 (fr) *	2018-05-29	2019-12-05	Capsenze Biosystems Ab	Procédé et système pour fournir des fragments d'anticorps convenant à un contrôle de qualité en ligne
KR102198342B1 (ko) *	2019-05-22	2021-01-04	연세대학교 산학협력단	항체 선별 방법 및 이를 이용한 항체 선별 시스템
DE102020113317A1 (de) *	2020-05-15	2021-11-18	Endress+Hauser Conducta Gmbh+Co. Kg	Automatische Probennahmevorrichtung und Verfahren zum automatisierten Bereitstellen einer Probe für eine qualitative und/oder quantitative Bestimmung eines Analyten

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5061790A (en) *	1989-07-10	1991-10-29	Molecular Diagnostics, Inc.	Oxidative denaturation of protein analytes
US6303325B1 (en) *	1998-05-29	2001-10-16	Dade Behring Inc.	Method for detecting analytes
CA2461587C (fr) *	2001-09-27	2011-04-26	Purdue Research Foundation	Materiaux et methodes pour controler les effets isotopiques pendant le fractionnement des analytes
JP2011519034A (ja) *	2008-04-23	2011-06-30	ルミネックスコーポレーション	生物学的起原の出発材料中に見出される複数の検体のスタンダードを作成する方法
WO2010132453A2 (fr) *	2009-05-11	2010-11-18	Nexus Dx, Inc.	Procédés et compositions pour une détection d'analyte
US8455202B2 (en) *	2010-03-10	2013-06-04	Perfinity Biosciences, Inc.	Affinity selector based recognition and quantification system and method for multiple analytes in a single analysis

2013
- 2013-01-30 EP EP13742988.2A patent/EP2820425A1/fr not_active Withdrawn
- 2013-01-30 IN IN6899DEN2014 patent/IN2014DN06899A/en unknown
- 2013-01-30 AU AU2013215305A patent/AU2013215305A1/en not_active Abandoned
- 2013-01-30 JP JP2014555635A patent/JP2015505619A/ja active Pending
- 2013-01-30 CN CN201380013470.5A patent/CN104160278A/zh active Pending
- 2013-01-30 WO PCT/US2013/023727 patent/WO2013116260A1/fr not_active Ceased
- 2013-01-30 SG SG11201404526PA patent/SG11201404526PA/en unknown
- 2013-01-30 KR KR20147024464A patent/KR20140137353A/ko not_active Withdrawn
- 2013-01-30 CA CA2863635A patent/CA2863635A1/fr not_active Abandoned
- 2013-01-30 BR BR112014019134A patent/BR112014019134A8/pt not_active IP Right Cessation
- 2013-01-30 RU RU2014135576A patent/RU2014135576A/ru unknown
2014
- 2014-07-31 IL IL233897A patent/IL233897A0/en unknown

Also Published As

Publication number	Publication date
JP2015505619A (ja)	2015-02-23
EP2820425A1 (fr)	2015-01-07
BR112014019134A8 (pt)	2017-07-11
SG11201404526PA (en)	2014-11-27
BR112014019134A2 (fr)	2017-06-20
KR20140137353A (ko)	2014-12-02
IN2014DN06899A (fr)	2015-05-15
IL233897A0 (en)	2014-09-30
WO2013116260A1 (fr)	2013-08-08
AU2013215305A1 (en)	2014-08-28
CN104160278A (zh)	2014-11-19
RU2014135576A (ru)	2016-03-27

Publication	Publication Date	Title
AU2010347764B2 (en)	2013-11-14	Method for recognition and quantification of multiple analytes in a single analysis
US10345293B2 (en)	2019-07-09	High sensitivity quantitation of peptides by mass spectrometry
US8455202B2 (en)	2013-06-04	Affinity selector based recognition and quantification system and method for multiple analytes in a single analysis
CN100558879C (zh)	2009-11-11	用质谱法高灵敏度定量肽
EP0835446B1 (fr)	2003-04-09	Selection et analyse moleculaires multidimensionnelles automatiques et rapides a flux continu
EP2286237B1 (fr)	2013-12-25	Analyse spectrométrique de masse
CA2863635A1 (fr)	2013-08-08	Systeme de reconnaissance et de quantification base sur des selecteurs et procede pour multiples analytes au cours d'une seule et meme analyse
JP7668411B2 (ja)	2025-04-24	無傷のタンパク質レベルでのＬＣ－ＭＳに基づくＨｂＡ１ｃ測定の自動化試料ワークフロー
US20150105280A1 (en)	2015-04-16	Selector based recognition and quantification system and method for multiple analytes in a single analysis
Liu et al.	2007	The use of a quantitative cysteinyl-peptide enrichment technology for high-throughput quantitative proteomics
Ohlson et al.	2016	Emerging technologies for fragment screening
US20080090298A1 (en)	2008-04-17	Method and system for identification of protein-protein interaction
EP1048950A1 (fr)	2000-11-02	Identification de ligands de récepteurs orphelins mettant en jeu un couplage entre des techniques de spectrométrie de masse et de séparation à flux continu
EP1300679A2 (fr)	2003-04-09	Sélection et analyse moléculaire

Legal Events

Date	Code	Title	Description
2016-02-23	FZDE	Discontinued	Effective date: 20151222