[go: up one dir, main page]

CA2861270C - Systemes de bioreacteur de perfusion comprenant un piege a agregat cellulaire et procede d'exploitation de ces systemes - Google Patents

Systemes de bioreacteur de perfusion comprenant un piege a agregat cellulaire et procede d'exploitation de ces systemes Download PDF

Info

Publication number
CA2861270C
CA2861270C CA2861270A CA2861270A CA2861270C CA 2861270 C CA2861270 C CA 2861270C CA 2861270 A CA2861270 A CA 2861270A CA 2861270 A CA2861270 A CA 2861270A CA 2861270 C CA2861270 C CA 2861270C
Authority
CA
Canada
Prior art keywords
cells
cell
trap
tissue culture
culture fluid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2861270A
Other languages
English (en)
Other versions
CA2861270A1 (fr
Inventor
Medhi Saghafi
Ricaredo Matanguihan
Chetan Goudar
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Healthcare LLC
Original Assignee
Bayer Healthcare LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Healthcare LLC filed Critical Bayer Healthcare LLC
Publication of CA2861270A1 publication Critical patent/CA2861270A1/fr
Application granted granted Critical
Publication of CA2861270C publication Critical patent/CA2861270C/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/22—Settling tanks; Sedimentation by gravity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10—Separation or concentration of fermentation products

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne un système de bioréacteur de perfusion comprenant un piège à agrégat cellulaire. Le piège à agrégat cellulaire est configuré pour retirer des agrégats de cellules (par exemple, des amas ou des agrégats supérieurs à environ 10 cellules) du système de bioréacteur à perfusion. Dans certains modes de réalisation, le piège à agrégat cellulaire a une chambre de sédimentation et une chambre d'écoulement latérale permettant à des agrégats de cellules de se séparer, d'être retirés et jetés. Il est prévu des procédés pour faire fonctionner le système de bioréacteur de perfusion ayant un piège à agrégat cellulaire, ainsi que de nombreux autres modes de réalisation.
CA2861270A 2012-01-18 2013-01-15 Systemes de bioreacteur de perfusion comprenant un piege a agregat cellulaire et procede d'exploitation de ces systemes Active CA2861270C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261587940P 2012-01-18 2012-01-18
US61/587,940 2012-01-18
PCT/US2013/021533 WO2013109520A1 (fr) 2012-01-18 2013-01-15 Systèmes de bioréacteur de perfusion et procédés d'exploitation de ces systèmes

Publications (2)

Publication Number Publication Date
CA2861270A1 CA2861270A1 (fr) 2013-07-25
CA2861270C true CA2861270C (fr) 2021-08-03

Family

ID=47605793

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2861270A Active CA2861270C (fr) 2012-01-18 2013-01-15 Systemes de bioreacteur de perfusion comprenant un piege a agregat cellulaire et procede d'exploitation de ces systemes

Country Status (5)

Country Link
EP (1) EP2804943A1 (fr)
JP (1) JP6227562B2 (fr)
CN (1) CN104160013B (fr)
CA (1) CA2861270C (fr)
WO (1) WO2013109520A1 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HUE055888T2 (hu) * 2013-09-16 2022-01-28 Genzyme Corp Eljárások és összeállítások sejttenyészet feldolgozására
JP6896242B2 (ja) * 2013-12-30 2021-06-30 グローバル・ライフ・サイエンシズ・ソリューションズ・ユーエスエー・エルエルシー 細胞培養のための装置
JP2017527299A (ja) * 2014-09-17 2017-09-21 マサチューセッツ インスティテュート オブ テクノロジー 灌流バイオリアクター細胞保持のためのマイクロ流体システム及び方法
US9944894B2 (en) * 2015-01-16 2018-04-17 General Electric Company Pluripotent stem cell expansion and passage using a rocking platform bioreactor
WO2017162467A1 (fr) * 2016-03-21 2017-09-28 General Electric Company Expansion et repiquage de cellules souches pluripotentes au moyen d'un bioréacteur à plateforme basculante
CN107541464B (zh) * 2017-10-31 2021-04-30 山东亦度生物技术有限公司 增强型的细胞微载体灌流培养的截留方法
US20210163868A1 (en) * 2018-05-22 2021-06-03 Nantkwest, Inc. Methods and systems for cell bed formation during bioprocessing
JP7087704B2 (ja) * 2018-06-13 2022-06-21 株式会社Ihi 細胞培養システム及び細胞培養方法
US11028359B2 (en) 2018-09-11 2021-06-08 Global Life Sciences Solutions Usa Llc Separation devices, associated methods, and systems
GB201904083D0 (en) * 2019-03-25 2019-05-08 Ge Healthcare Bio Sciences Ab A bioreactor system
EP4055136A1 (fr) * 2019-11-07 2022-09-14 Merck Patent GmbH Procédés et systèmes pour réaliser une culture de cellules de perfusion
EP3835403A1 (fr) * 2019-12-11 2021-06-16 Sartorius Stedim Biotech GmbH Stratégie d'échange automatique de support pour cellules de suspension
JP7406205B2 (ja) * 2020-03-24 2023-12-27 横河電機株式会社 メタン生成装置
EP4608965A1 (fr) * 2022-10-26 2025-09-03 National Resilience, Inc. Systèmes de bioréacteur et leurs procédés de fonctionnement

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05153973A (ja) * 1991-12-03 1993-06-22 Hitachi Ltd 細胞培養方法及び装置
US6338964B1 (en) 1999-05-07 2002-01-15 Bayer Corporation Process and medium for mammalian cell culture under low dissolved carbon dioxide concentration
CN1112442C (zh) * 2000-06-14 2003-06-25 华东理工大学 细胞灌注培养过程中的细胞截留分离方法及其装置
WO2003020919A2 (fr) * 2001-08-31 2003-03-13 Bayer Healthcare Ag Unite et procede de fermentation a haute densite cellulaire
DK1807101T3 (en) 2004-09-30 2016-05-23 Bayer Healthcare Llc Devices and methods for integrated continuous production of biological molecules
WO2007071072A1 (fr) * 2005-12-22 2007-06-28 Corporation De L'ecole Polytechnique De Montreal Bioréacteur de perfusion à haut rendement
US8512566B2 (en) * 2009-12-11 2013-08-20 General Electric Company Disposable fluid path systems and methods for processing complex biological materials

Also Published As

Publication number Publication date
JP6227562B2 (ja) 2017-11-08
HK1199056A1 (en) 2015-06-19
CA2861270A1 (fr) 2013-07-25
WO2013109520A1 (fr) 2013-07-25
CN104160013A (zh) 2014-11-19
EP2804943A1 (fr) 2014-11-26
CN104160013B (zh) 2017-07-28
JP2015503929A (ja) 2015-02-05

Similar Documents

Publication Publication Date Title
CA2861270C (fr) Systemes de bioreacteur de perfusion comprenant un piege a agregat cellulaire et procede d'exploitation de ces systemes
JP7102391B2 (ja) 交互タンジェンシャルフローによる高速収穫
Birch et al. Suspension culture of mammalian cells
EA016451B1 (ru) Способ суспензионного культивирования клеток
Coronel et al. Application of an inclined settler for cell culture-based influenza A virus production in perfusion mode
JP6282467B2 (ja) タンパク質を生産するための方法
CN103773741B (zh) 笼式通气搅拌生物反应器制备流感疫苗的方法
AU2019201507A1 (en) A high cell density fill and draw fermentation process
IL229058A (en) A method for increasing the productivity of eukaryotic cells in the production of recombinant fviii
JP5096920B2 (ja) ウイルス物質の製造方法
CN114317392A (zh) 优化细胞培养的方法
HK1199056B (en) Perfusion bioreactor systems and methods of operating the same
WO2010003759A2 (fr) Procédé de culture de cellules
Ahmed Application of hydrocyclone for cell separation in mammalian cell perfusion cultures
CN116162602A (zh) 一种新型的从微载体收获产物的方法
ARATHOON JOHN R. BIRCH
CN119365583A (zh) 用于细胞培养的系统、设备和方法
HK1102608B (en) Method for preparing viral material

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20180111