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CA2712426A1 - Sequencage d'extremites par paire - Google Patents

Sequencage d'extremites par paire Download PDF

Info

Publication number
CA2712426A1
CA2712426A1 CA2712426A CA2712426A CA2712426A1 CA 2712426 A1 CA2712426 A1 CA 2712426A1 CA 2712426 A CA2712426 A CA 2712426A CA 2712426 A CA2712426 A CA 2712426A CA 2712426 A1 CA2712426 A1 CA 2712426A1
Authority
CA
Canada
Prior art keywords
nucleic acid
dna
adaptor
target nucleic
sequencing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2712426A
Other languages
English (en)
Inventor
Zhoutao Chen
Brian Christopher Godwin
Gianni Calogero Ferreri
David Roderick Riches
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2712426A1 publication Critical patent/CA2712426A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2712426A 2008-02-05 2009-02-04 Sequencage d'extremites par paire Abandoned CA2712426A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US2631908P 2008-02-05 2008-02-05
US61/026,319 2008-02-05
PCT/EP2009/000741 WO2009098037A1 (fr) 2008-02-05 2009-02-04 Séquençage d'extrémités par paire

Publications (1)

Publication Number Publication Date
CA2712426A1 true CA2712426A1 (fr) 2009-08-13

Family

ID=40577731

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2712426A Abandoned CA2712426A1 (fr) 2008-02-05 2009-02-04 Sequencage d'extremites par paire

Country Status (5)

Country Link
EP (1) EP2242855A1 (fr)
JP (1) JP2011510669A (fr)
CN (1) CN102027130A (fr)
CA (1) CA2712426A1 (fr)
WO (1) WO2009098037A1 (fr)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
US9334531B2 (en) 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
WO2012029577A1 (fr) * 2010-09-02 2012-03-08 学校法人 久留米大学 Procédé de production d'adn circulaire à partir d'adn monomoléculaire
CN118086471A (zh) 2010-12-17 2024-05-28 生命技术公司 用于核酸扩增的方法、组合物、系统、仪器和试剂盒
EP2752486B1 (fr) * 2011-08-31 2016-12-28 Kurume University Procédé de sélection exclusive de l'adn circularisé à partir d'un adn monomoléculaire lors de la circularisation de molécules d'adn
JP6093498B2 (ja) * 2011-12-13 2017-03-08 株式会社日立ハイテクノロジーズ 核酸増幅方法
JP2015526092A (ja) * 2012-08-24 2015-09-10 ライフ テクノロジーズ コーポレーション 核酸ペアエンド配列決定のための方法、組成物、システム、装置、およびキット
JP6422193B2 (ja) * 2013-09-30 2018-11-14 キアゲン ゲーエムベーハー Ｄｎａライブラリーの調製のためのｄｎａアダプター分子およびその生成法および使用
CN106029962A (zh) * 2014-02-11 2016-10-12 豪夫迈·罗氏有限公司 靶向测序和uid过滤
GB201416106D0 (en) * 2014-09-11 2014-10-29 Illumina Cambridge Ltd Paired-end sequencing by attachment of the complementary strand
JP2018509928A (ja) * 2015-04-02 2018-04-12 ザ ジャクソン ラボラトリー 環状化メイトペアライブラリーおよびショットガン配列決定を用いて、ゲノム変異を検出するための方法
EP3378975B1 (fr) * 2015-11-17 2021-01-27 Zhejiang Annoroad Bio-Technology Co., Ltd. Procédé pour construire une bibliothèque d'adn pour le séquençage
EP3491147B1 (fr) 2016-08-01 2020-06-17 H. Hoffnabb-La Roche Ag Procédés de suppression de dimères d'adaptateurs dans des préparations de séquençage d'acides nucléiques
JP6931540B2 (ja) * 2017-02-27 2021-09-08 シスメックス株式会社 検体処理チップを用いた送液方法、検体処理チップの送液装置
EP3913053A1 (fr) * 2017-04-23 2021-11-24 Illumina Cambridge Limited Compositions et procédés permettant d'améliorer l'identification d'échantillons dans des bibliothèques d'acides nucléiques indexés
WO2018200386A1 (fr) 2017-04-23 2018-11-01 Illumina, Inc. Compositions et procédés pour améliorer l'identification d'échantillons dans des bibliothèques d'acides nucléiques indexés
WO2019032762A1 (fr) * 2017-08-10 2019-02-14 Rootpath Genomics, Inc. Procédés pour améliorer le séquençage de polynucléotides à l'aide de codes-barres en utilisant une circularisation et une troncature de matrice
EP3704059A4 (fr) * 2017-11-03 2022-06-29 The University of Washington Amplification d'acide nucléique numérique à l'aide de particules codées
WO2020058389A1 (fr) * 2018-09-21 2020-03-26 F. Hoffmann-La Roche Ag Système et procédé de préparation d'échantillon d'acide nucléique modulaire et combinatoire pour séquençage

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7517688B2 (en) * 2000-02-25 2009-04-14 Stratagene California Method for ligating nucleic acids and molecular cloning
CA2615323A1 (fr) * 2005-06-06 2007-12-21 454 Life Sciences Corporation Sequencage d'extremites appariees

Also Published As

Publication number Publication date
CN102027130A (zh) 2011-04-20
EP2242855A1 (fr) 2010-10-27
JP2011510669A (ja) 2011-04-07
WO2009098037A1 (fr) 2009-08-13

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued

Effective date: 20130204