CA2700545A1 - Gene innovant implique dans la biosynthese de l'acide petroselinique et procede de production d'acide petroselinique - Google Patents
Gene innovant implique dans la biosynthese de l'acide petroselinique et procede de production d'acide petroselinique Download PDFInfo
- Publication number
- CA2700545A1 CA2700545A1 CA2700545A CA2700545A CA2700545A1 CA 2700545 A1 CA2700545 A1 CA 2700545A1 CA 2700545 A CA2700545 A CA 2700545A CA 2700545 A CA2700545 A CA 2700545A CA 2700545 A1 CA2700545 A1 CA 2700545A1
- Authority
- CA
- Canada
- Prior art keywords
- acid
- transformed plant
- producing
- petroselinic acid
- gene according
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- CNVZJPUDSLNTQU-SEYXRHQNSA-N petroselinic acid Chemical compound CCCCCCCCCCC\C=C/CCCCC(O)=O CNVZJPUDSLNTQU-SEYXRHQNSA-N 0.000 title claims abstract 18
- 108090000623 proteins and genes Proteins 0.000 title claims abstract 17
- OXEDXHIBHVMDST-UHFFFAOYSA-N 12Z-octadecenoic acid Natural products CCCCCC=CCCCCCCCCCCC(O)=O OXEDXHIBHVMDST-UHFFFAOYSA-N 0.000 title claims abstract 9
- CNVZJPUDSLNTQU-UHFFFAOYSA-N Petroselaidic acid Natural products CCCCCCCCCCCC=CCCCCC(O)=O CNVZJPUDSLNTQU-UHFFFAOYSA-N 0.000 title claims abstract 9
- AQWHMKSIVLSRNY-UHFFFAOYSA-N trans-Octadec-5-ensaeure Natural products CCCCCCCCCCCCC=CCCCC(O)=O AQWHMKSIVLSRNY-UHFFFAOYSA-N 0.000 title claims abstract 9
- 238000004519 manufacturing process Methods 0.000 title claims abstract 8
- 230000015572 biosynthetic process Effects 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 claims abstract 4
- 102000005488 Thioesterase Human genes 0.000 claims abstract 3
- 125000003275 alpha amino acid group Chemical group 0.000 claims abstract 3
- 230000000694 effects Effects 0.000 claims abstract 3
- 108020002982 thioesterase Proteins 0.000 claims abstract 3
- 238000007792 addition Methods 0.000 claims abstract 2
- 150000001413 amino acids Chemical class 0.000 claims abstract 2
- 238000012217 deletion Methods 0.000 claims abstract 2
- 230000037430 deletion Effects 0.000 claims abstract 2
- 238000006467 substitution reaction Methods 0.000 claims abstract 2
- 230000001131 transforming effect Effects 0.000 claims 4
- 150000004671 saturated fatty acids Chemical class 0.000 claims 3
- OXGMPGKZDZPDIF-SEYXRHQNSA-N (4Z)-hexadecenoic acid Chemical compound CCCCCCCCCCC\C=C/CCC(O)=O OXGMPGKZDZPDIF-SEYXRHQNSA-N 0.000 claims 2
- 239000013604 expression vector Substances 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 2
- 125000003729 nucleotide group Chemical group 0.000 claims 2
- 230000000295 complement effect Effects 0.000 claims 1
- 238000009825 accumulation Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
- C12N9/0083—Miscellaneous (1.14.99)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Nutrition Science (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
On présente de nouveaux gènes capables de promouvoir l'accumulation d'acide pétrosélinique ainsi qu'une méthode permettant de produire de l'acide pétrosélinique à l'aide des gènes en question. Ces nouveaux gènes codent une protéine contenant la séquence d'acides aminés indiquée dans la séquence no 4 ou 6, laquelle peut comprendre la délétion, la substitution ou l'addition d'un ou de plusieurs acide(s) aminé(s) et qui possède une activité pétrosélinoyl-ACP thioestérase. On décrit également des méthodes permettant de produire de l'acide pétrosélinique ainsi que des cellules végétales modifiées.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2005-191775 | 2005-06-30 | ||
| JP2005191775 | 2005-06-30 | ||
| CA2614062A CA2614062C (fr) | 2005-06-30 | 2006-06-29 | Gene innovant implique dans la biosynthese de l'acide petroselinique et procede de production d'acide petroselinique |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2614062A Division CA2614062C (fr) | 2005-06-30 | 2006-06-29 | Gene innovant implique dans la biosynthese de l'acide petroselinique et procede de production d'acide petroselinique |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2700545A1 true CA2700545A1 (fr) | 2007-01-11 |
| CA2700545C CA2700545C (fr) | 2012-08-28 |
Family
ID=37604552
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2614062A Active CA2614062C (fr) | 2005-06-30 | 2006-06-29 | Gene innovant implique dans la biosynthese de l'acide petroselinique et procede de production d'acide petroselinique |
| CA2700545A Active CA2700545C (fr) | 2005-06-30 | 2006-06-29 | Gene innovant implique dans la biosynthese de l'acide petroselinique et procede de production d'acide petroselinique |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2614062A Active CA2614062C (fr) | 2005-06-30 | 2006-06-29 | Gene innovant implique dans la biosynthese de l'acide petroselinique et procede de production d'acide petroselinique |
Country Status (5)
| Country | Link |
|---|---|
| JP (1) | JP4840360B2 (fr) |
| CN (1) | CN101213298B (fr) |
| AU (1) | AU2006266760B2 (fr) |
| CA (2) | CA2614062C (fr) |
| WO (1) | WO2007004694A1 (fr) |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5430134A (en) * | 1992-08-07 | 1995-07-04 | Michigan State University | Method for production of petroselinic acid and OMEGA12 hexadecanoic acid in transgenic plants |
| MX9701238A (es) * | 1994-08-31 | 1997-05-31 | Du Pont | Secuencias de nucleotidos de genes de palmitoil-acp tiosterasa de nabina (canola) y soya y uso en la regulacion del contenido de acidos grasos en los acidos de plantas de soya y nabina (canola). |
| US6150512A (en) * | 1995-05-15 | 2000-11-21 | Yuan; Ling | Engineering plant thioesterases and disclosure of plant thioesterases having novel substrate specificity |
| EP0795020A4 (fr) * | 1995-09-29 | 1999-02-24 | Calgene Inc | Sequences de stearoyl-(proteine vectrice d'acyle)-thioesterase vegetales et procedes pour augmenter la teneur en stearate des huiles de graines vegetales |
| US6281375B1 (en) * | 1998-08-03 | 2001-08-28 | Cargill, Incorporated | Biodegradable high oxidative stability oils |
| GB9918022D0 (en) * | 1999-07-30 | 1999-09-29 | Unilever Plc | Skin care composition |
-
2006
- 2006-06-29 CA CA2614062A patent/CA2614062C/fr active Active
- 2006-06-29 WO PCT/JP2006/313449 patent/WO2007004694A1/fr not_active Ceased
- 2006-06-29 JP JP2007523443A patent/JP4840360B2/ja not_active Expired - Fee Related
- 2006-06-29 CA CA2700545A patent/CA2700545C/fr active Active
- 2006-06-29 CN CN2006800241478A patent/CN101213298B/zh not_active Expired - Fee Related
- 2006-06-29 AU AU2006266760A patent/AU2006266760B2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO2007004694A1 (fr) | 2007-01-11 |
| AU2006266760A1 (en) | 2007-01-11 |
| JPWO2007004694A1 (ja) | 2009-01-29 |
| CN101213298B (zh) | 2012-04-18 |
| JP4840360B2 (ja) | 2011-12-21 |
| AU2006266760B2 (en) | 2011-12-15 |
| CA2614062A1 (fr) | 2007-01-11 |
| CA2614062C (fr) | 2011-11-15 |
| CN101213298A (zh) | 2008-07-02 |
| CA2700545C (fr) | 2012-08-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |