CA2630305A1 - A novel gene encoding amorphadiene hydroxylase and a process to produce artemisinic alcohol therewith - Google Patents
A novel gene encoding amorphadiene hydroxylase and a process to produce artemisinic alcohol therewith Download PDFInfo
- Publication number
- CA2630305A1 CA2630305A1 CA002630305A CA2630305A CA2630305A1 CA 2630305 A1 CA2630305 A1 CA 2630305A1 CA 002630305 A CA002630305 A CA 002630305A CA 2630305 A CA2630305 A CA 2630305A CA 2630305 A1 CA2630305 A1 CA 2630305A1
- Authority
- CA
- Canada
- Prior art keywords
- seq
- set forth
- amino acid
- acid sequence
- artemisinic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
- C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/22—Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The isolation and characterization of amorphadiene hydroxylases capable of converting amorpha-4, 1 1 -diene to artemisinic alcohol or artemisinic acid is herein described.
Claims (14)
1. An isolated or purified nucleic acid molecule encoding a peptide having at least 85% identity with an amino acid sequence as set forth in SEQ ID No. 2 or SEQ ID
No. 6, as discussed above.
No. 6, as discussed above.
2. The nucleic acid molecule according to claim 1 wherein the amino acid sequence is as set forth in SEQ ID No. 2.
3. The nucleic acid molecule according to claim 1 wherein the amino acid sequence is as set forth in SEQ ID No. 6.
4. An isolated or purified nucleic acid molecule encoding an amorphadiene hydroxylase peptide having at least 85% identity with an amino acid sequence as set forth in SEQ ID No. 2 or SEQ ID No. 6.
5. The nucleic acid molecule according to claim 4 having an amino acid sequence as set forth in SEQ ID No. 2.
6. The nucleic acid molecule according to claim 4 having an amino acid sequence as set forth in SEQ ID No. 6.
7. A method of producing artemisinic acid comprising:
providing a host organism comprising a vector having a nucleic acid molecule encoding an amorphadiene hydroxylase peptide having at least 85%
identity with an amino acid sequence as set forth in SEQ ID No. 2 or SEQ ID No. 6 operably linked to a suitable host-operable promoter;
incubating said host organism with amorpha-4,11-diene under conditions wherein the amorphadiene hydroxylase peptide is expressed; and recovering artemisinic alcohol or artemisinic acid.
providing a host organism comprising a vector having a nucleic acid molecule encoding an amorphadiene hydroxylase peptide having at least 85%
identity with an amino acid sequence as set forth in SEQ ID No. 2 or SEQ ID No. 6 operably linked to a suitable host-operable promoter;
incubating said host organism with amorpha-4,11-diene under conditions wherein the amorphadiene hydroxylase peptide is expressed; and recovering artemisinic alcohol or artemisinic acid.
8. The method according to claim 7 wherein the host organism is yeast.
9. The method according to claim 7 wherein the amino acid sequence is as set forth in SEQ ID No. 2.
10. The method according to claim 7 wherein the amino acid sequence is as set forth in SEQ ID No. 6.
11. A method of producing artemisinic acid comprising:
providing a host organism comprising a vector having a nucleic acid molecule encoding an amorphadiene hydroxylase peptide having at least 85%
identity with an amino acid sequence as set forth in SEQ ID No. 2 or SEQ ID No. 6 operably linked to a suitable host-operable promoter;
incubating said host organism with an artemisinic acid precursor under conditions wherein the amorphadiene hydroxylase peptide is expressed; and recovering artemisinic alcohol or artemisinic acid.
providing a host organism comprising a vector having a nucleic acid molecule encoding an amorphadiene hydroxylase peptide having at least 85%
identity with an amino acid sequence as set forth in SEQ ID No. 2 or SEQ ID No. 6 operably linked to a suitable host-operable promoter;
incubating said host organism with an artemisinic acid precursor under conditions wherein the amorphadiene hydroxylase peptide is expressed; and recovering artemisinic alcohol or artemisinic acid.
12. The method according to claim 11 wherein the host organism is yeast.
13. The method according to claim 11 wherein the amino acid sequence is as set forth in SEQ ID No. 2.
14. The method according to claim 11 wherein the amino acid sequence is as set forth in SEQ ID No. 6.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US72921005P | 2005-10-24 | 2005-10-24 | |
| US60/729,210 | 2005-10-24 | ||
| PCT/CA2006/001746 WO2007048235A1 (en) | 2005-10-24 | 2006-10-24 | A novel gene encoding amorphadiene hydroxylase and a process to produce artemisinic alcohol therewith |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2630305A1 true CA2630305A1 (en) | 2007-05-03 |
Family
ID=37967373
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002630305A Abandoned CA2630305A1 (en) | 2005-10-24 | 2006-10-24 | A novel gene encoding amorphadiene hydroxylase and a process to produce artemisinic alcohol therewith |
Country Status (2)
| Country | Link |
|---|---|
| CA (1) | CA2630305A1 (en) |
| WO (1) | WO2007048235A1 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AP3827A (en) * | 2005-07-05 | 2016-09-30 | Univ California | Polynucleotides encoding isoprenoid modifying enzymes and methods of use thereof |
| CN119552835B (en) * | 2024-12-05 | 2025-07-01 | 暨南大学 | Novel application of deoxyartemisinin B synthase and isozymes A0A2U1PPI9 thereof |
-
2006
- 2006-10-24 WO PCT/CA2006/001746 patent/WO2007048235A1/en not_active Ceased
- 2006-10-24 CA CA002630305A patent/CA2630305A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2007048235A1 (en) | 2007-05-03 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Dead |
Effective date: 20140702 |