CA2611071A1 - Hydrolases, acides nucleiques codant pour celles-ci et procedes de fabrication et d'utilisation de celles-ci - Google Patents

Hydrolases, acides nucleiques codant pour celles-ci et procedes de fabrication et d'utilisation de celles-ci Download PDF

Info

Publication number: CA2611071A1
Authority: CA; Canada
Prior art keywords: polypeptide; nucleic acid; hydrolase; sequence; seq
Prior art date: 2005-05-31
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Abandoned

Application number

CA002611071A

Other languages

English (en)

Inventor

Eric Mathur

Walter Callen

Roderick Fielding

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

BASF Enzymes LLC

Original Assignee

Verenium Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2005-05-31

Filing date

2006-05-23

Publication date

2007-05-18

2006-05-23 Application filed by Verenium Corp filed Critical Verenium Corp

2007-05-18 Publication of CA2611071A1 publication Critical patent/CA2611071A1/fr

Status Abandoned legal-status Critical Current

Links

108090000604 Hydrolases Proteins 0.000 title claims abstract description 345
102000004157 Hydrolases Human genes 0.000 title claims abstract description 343
238000000034 method Methods 0.000 title claims abstract description 286
150000007523 nucleic acids Chemical class 0.000 title claims description 356
102000039446 nucleic acids Human genes 0.000 title claims description 307
108020004707 nucleic acids Proteins 0.000 title claims description 307
108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 444
102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 427
229920001184 polypeptide Polymers 0.000 claims abstract description 415
230000000694 effects Effects 0.000 claims abstract description 276
108090001060 Lipase Proteins 0.000 claims abstract description 81
102000004882 Lipase Human genes 0.000 claims abstract description 81
239000004367 Lipase Substances 0.000 claims abstract description 81
235000019421 lipase Nutrition 0.000 claims abstract description 81
150000002148 esters Chemical class 0.000 claims abstract description 79
102000004190 Enzymes Human genes 0.000 claims abstract description 76
108090000790 Enzymes Proteins 0.000 claims abstract description 76
235000014113 dietary fatty acids Nutrition 0.000 claims abstract description 63
229930195729 fatty acid Natural products 0.000 claims abstract description 63
239000000194 fatty acid Substances 0.000 claims abstract description 63
102000015439 Phospholipases Human genes 0.000 claims abstract description 60
108010064785 Phospholipases Proteins 0.000 claims abstract description 60
150000004665 fatty acids Chemical class 0.000 claims abstract description 57
-1 e.g. Proteins 0.000 claims abstract description 51
ZIIUUSVHCHPIQD-UHFFFAOYSA-N 2,4,6-trimethyl-N-[3-(trifluoromethyl)phenyl]benzenesulfonamide Chemical compound CC1=CC(C)=CC(C)=C1S(=O)(=O)NC1=CC=CC(C(F)(F)F)=C1 ZIIUUSVHCHPIQD-UHFFFAOYSA-N 0.000 claims abstract description 50
238000006460 hydrolysis reaction Methods 0.000 claims abstract description 48
108091005804 Peptidases Proteins 0.000 claims abstract description 47
230000007062 hydrolysis Effects 0.000 claims abstract description 47
108090000371 Esterases Proteins 0.000 claims abstract description 45
239000004365 Protease Substances 0.000 claims abstract description 44
150000002632 lipids Chemical class 0.000 claims abstract description 44
238000006243 chemical reaction Methods 0.000 claims abstract description 41
235000019626 lipase activity Nutrition 0.000 claims abstract description 40
150000003626 triacylglycerols Chemical class 0.000 claims abstract description 39
238000003786 synthesis reaction Methods 0.000 claims abstract description 35
102000040430 polynucleotide Human genes 0.000 claims abstract description 33
108091033319 polynucleotide Proteins 0.000 claims abstract description 33
239000002157 polynucleotide Substances 0.000 claims abstract description 33
230000015572 biosynthetic process Effects 0.000 claims abstract description 31
235000021588 free fatty acids Nutrition 0.000 claims abstract description 27
238000004519 manufacturing process Methods 0.000 claims abstract description 18
238000005809 transesterification reaction Methods 0.000 claims abstract description 16
102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract 4
210000004027 cell Anatomy 0.000 claims description 146
239000000203 mixture Substances 0.000 claims description 130
241000196324 Embryophyta Species 0.000 claims description 82
108090000623 proteins and genes Proteins 0.000 claims description 76
239000000758 substrate Substances 0.000 claims description 74
238000002703 mutagenesis Methods 0.000 claims description 72
231100000350 mutagenesis Toxicity 0.000 claims description 72
108020004705 Codon Proteins 0.000 claims description 63
230000014509 gene expression Effects 0.000 claims description 60
235000019198 oils Nutrition 0.000 claims description 60
239000003921 oil Substances 0.000 claims description 59
235000020777 polyunsaturated fatty acids Nutrition 0.000 claims description 57
239000000523 sample Substances 0.000 claims description 57
108091028043 Nucleic acid sequence Proteins 0.000 claims description 50
238000012360 testing method Methods 0.000 claims description 49
102000004169 proteins and genes Human genes 0.000 claims description 35
239000013598 vector Substances 0.000 claims description 35
150000001875 compounds Chemical class 0.000 claims description 34
230000007613 environmental effect Effects 0.000 claims description 34
150000001982 diacylglycerols Chemical class 0.000 claims description 32
DCXXMTOCNZCJGO-UHFFFAOYSA-N tristearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(OC(=O)CCCCCCCCCCCCCCCCC)COC(=O)CCCCCCCCCCCCCCCCC DCXXMTOCNZCJGO-UHFFFAOYSA-N 0.000 claims description 32
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 30
235000019197 fats Nutrition 0.000 claims description 30
150000003384 small molecules Chemical class 0.000 claims description 30
150000001413 amino acids Chemical class 0.000 claims description 28
239000007795 chemical reaction product Substances 0.000 claims description 26
108091034117 Oligonucleotide Proteins 0.000 claims description 25
239000002253 acid Substances 0.000 claims description 23
230000007423 decrease Effects 0.000 claims description 23
XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 claims description 22
235000015112 vegetable and seed oil Nutrition 0.000 claims description 22
239000008158 vegetable oil Substances 0.000 claims description 22
239000004744 fabric Substances 0.000 claims description 21
210000001519 tissue Anatomy 0.000 claims description 21
235000013305 food Nutrition 0.000 claims description 20
108020004414 DNA Proteins 0.000 claims description 19
230000003321 amplification Effects 0.000 claims description 19
239000012634 fragment Substances 0.000 claims description 19
238000003199 nucleic acid amplification method Methods 0.000 claims description 19
230000002210 biocatalytic effect Effects 0.000 claims description 18
230000003301 hydrolyzing effect Effects 0.000 claims description 18
230000009261 transgenic effect Effects 0.000 claims description 18
QXPXMOHHFYONAC-FEKLXSIDSA-N 1-palmitoyl-2-oleoyl-3-stearoyl-sn-glycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCCCCCCCCC)OC(=O)CCCCCCC\C=C/CCCCCCCC QXPXMOHHFYONAC-FEKLXSIDSA-N 0.000 claims description 17
238000004422 calculation algorithm Methods 0.000 claims description 17
QXPXMOHHFYONAC-UHFFFAOYSA-N rac-glyceryl-1-palmitate-2-oleate-3-stearate Natural products CCCCCCCCCCCCCCCCCC(=O)OCC(COC(=O)CCCCCCCCCCCCCCC)OC(=O)CCCCCCCC=CCCCCCCCC QXPXMOHHFYONAC-UHFFFAOYSA-N 0.000 claims description 17
240000008042 Zea mays Species 0.000 claims description 16
235000002017 Zea mays subsp mays Nutrition 0.000 claims description 16
230000027455 binding Effects 0.000 claims description 16
239000000047 product Substances 0.000 claims description 16
108020001507 fusion proteins Proteins 0.000 claims description 15
102000037865 fusion proteins Human genes 0.000 claims description 15
241001465754 Metazoa Species 0.000 claims description 14
238000009396 hybridization Methods 0.000 claims description 14
QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 claims description 14
238000004458 analytical method Methods 0.000 claims description 13
239000000463 material Substances 0.000 claims description 13
235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 12
238000004590 computer program Methods 0.000 claims description 12
235000005822 corn Nutrition 0.000 claims description 12
238000012217 deletion Methods 0.000 claims description 12
230000037430 deletion Effects 0.000 claims description 12
235000007882 dietary composition Nutrition 0.000 claims description 12
230000009368 gene silencing by RNA Effects 0.000 claims description 12
230000004048 modification Effects 0.000 claims description 12
238000012986 modification Methods 0.000 claims description 12
108010076504 Protein Sorting Signals Proteins 0.000 claims description 11
235000019868 cocoa butter Nutrition 0.000 claims description 11
229940110456 cocoa butter Drugs 0.000 claims description 11
239000003599 detergent Substances 0.000 claims description 11
230000002401 inhibitory effect Effects 0.000 claims description 11
239000000314 lubricant Substances 0.000 claims description 11
230000003287 optical effect Effects 0.000 claims description 11
235000019260 propionic acid Nutrition 0.000 claims description 11
238000000746 purification Methods 0.000 claims description 11
IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 claims description 11
229920006395 saturated elastomer Polymers 0.000 claims description 11
235000007164 Oryza sativa Nutrition 0.000 claims description 10
244000061456 Solanum tuberosum Species 0.000 claims description 10
235000021355 Stearic acid Nutrition 0.000 claims description 10
241000209140 Triticum Species 0.000 claims description 10
230000001580 bacterial effect Effects 0.000 claims description 10
235000019875 cocoa butter alternative Nutrition 0.000 claims description 10
230000001965 increasing effect Effects 0.000 claims description 10
239000004816 latex Substances 0.000 claims description 10
229920000126 latex Polymers 0.000 claims description 10
OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 claims description 10
238000002360 preparation method Methods 0.000 claims description 10
235000009566 rice Nutrition 0.000 claims description 10
238000002741 site-directed mutagenesis Methods 0.000 claims description 10
239000008117 stearic acid Substances 0.000 claims description 10
239000000126 substance Substances 0.000 claims description 10
235000004977 Brassica sinapistrum Nutrition 0.000 claims description 9
108091026890 Coding region Proteins 0.000 claims description 9
238000010367 cloning Methods 0.000 claims description 9
239000013604 expression vector Substances 0.000 claims description 9
239000002773 nucleotide Substances 0.000 claims description 9
125000003729 nucleotide group Chemical group 0.000 claims description 9
238000011282 treatment Methods 0.000 claims description 9
235000010469 Glycine max Nutrition 0.000 claims description 8
235000002637 Nicotiana tabacum Nutrition 0.000 claims description 8
235000002595 Solanum tuberosum Nutrition 0.000 claims description 8
235000021307 Triticum Nutrition 0.000 claims description 8
241000700605 Viruses Species 0.000 claims description 8
125000002252 acyl group Chemical group 0.000 claims description 8
238000007792 addition Methods 0.000 claims description 8
230000000295 complement effect Effects 0.000 claims description 8
239000002158 endotoxin Substances 0.000 claims description 8
238000006911 enzymatic reaction Methods 0.000 claims description 8
238000009884 interesterification Methods 0.000 claims description 8
239000007788 liquid Substances 0.000 claims description 8
230000006798 recombination Effects 0.000 claims description 8
238000005215 recombination Methods 0.000 claims description 8
239000003981 vehicle Substances 0.000 claims description 8
238000011179 visual inspection Methods 0.000 claims description 8
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
FDCOHGHEADZEGF-UHFFFAOYSA-N (E)-Glycerol 1,3-dihexadecanoate 2-9-octadecenoate Natural products CCCCCCCCCCCCCCCC(=O)OCC(COC(=O)CCCCCCCCCCCCCCC)OC(=O)CCCCCCCC=CCCCCCCCC FDCOHGHEADZEGF-UHFFFAOYSA-N 0.000 claims description 7
RBLADLVPSYELCA-UHFFFAOYSA-N (Z)-Glycerol 1,3-dioctadecanoate 2-9-octadecenoate Natural products CCCCCCCCCCCCCCCCCC(=O)OCC(OC(=O)CCCCCCCC=CCCCCCCCC)COC(=O)CCCCCCCCCCCCCCCCC RBLADLVPSYELCA-UHFFFAOYSA-N 0.000 claims description 7
RBLADLVPSYELCA-IKPAITLHSA-N 1,3-bis(octadecanoyloxy)propan-2-yl (9z)-octadec-9-enoate Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(OC(=O)CCCCCCC\C=C/CCCCCCCC)COC(=O)CCCCCCCCCCCCCCCCC RBLADLVPSYELCA-IKPAITLHSA-N 0.000 claims description 7
FDCOHGHEADZEGF-QPLCGJKRSA-N 1,3-dipalmitoyl-2-oleoylglycerol Chemical compound CCCCCCCCCCCCCCCC(=O)OCC(COC(=O)CCCCCCCCCCCCCCC)OC(=O)CCCCCCC\C=C/CCCCCCCC FDCOHGHEADZEGF-QPLCGJKRSA-N 0.000 claims description 7
240000002791 Brassica napus Species 0.000 claims description 7
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 7
244000068988 Glycine max Species 0.000 claims description 7
241000238631 Hexapoda Species 0.000 claims description 7
235000007340 Hordeum vulgare Nutrition 0.000 claims description 7
150000007513 acids Chemical class 0.000 claims description 7
230000032050 esterification Effects 0.000 claims description 7
238000005886 esterification reaction Methods 0.000 claims description 7
239000000835 fiber Substances 0.000 claims description 7
239000000499 gel Substances 0.000 claims description 7
210000004962 mammalian cell Anatomy 0.000 claims description 7
239000013612 plasmid Substances 0.000 claims description 7
230000002194 synthesizing effect Effects 0.000 claims description 7
238000012384 transportation and delivery Methods 0.000 claims description 7
UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 claims description 7
RSDIQTNCDWUNDP-VHXPQNKSSA-N 2,3-bis(tetradecanoyloxy)propyl (9z)-octadec-9-enoate Chemical compound CCCCCCCCCCCCCC(=O)OCC(OC(=O)CCCCCCCCCCCCC)COC(=O)CCCCCCC\C=C/CCCCCCCC RSDIQTNCDWUNDP-VHXPQNKSSA-N 0.000 claims description 6
229920000742 Cotton Polymers 0.000 claims description 6
108020004711 Nucleic Acid Probes Proteins 0.000 claims description 6
ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims description 6
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims description 6
150000001298 alcohols Chemical class 0.000 claims description 6
JAZBEHYOTPTENJ-JLNKQSITSA-N all-cis-5,8,11,14,17-icosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O JAZBEHYOTPTENJ-JLNKQSITSA-N 0.000 claims description 6
239000003674 animal food additive Substances 0.000 claims description 6
239000000074 antisense oligonucleotide Substances 0.000 claims description 6
238000012230 antisense oligonucleotides Methods 0.000 claims description 6
YZXBAPSDXZZRGB-DOFZRALJSA-N arachidonic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YZXBAPSDXZZRGB-DOFZRALJSA-N 0.000 claims description 6
210000004436 artificial bacterial chromosome Anatomy 0.000 claims description 6
210000001106 artificial yeast chromosome Anatomy 0.000 claims description 6
238000013500 data storage Methods 0.000 claims description 6
235000020673 eicosapentaenoic acid Nutrition 0.000 claims description 6
JAZBEHYOTPTENJ-UHFFFAOYSA-N eicosapentaenoic acid Natural products CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O JAZBEHYOTPTENJ-UHFFFAOYSA-N 0.000 claims description 6
229960005135 eicosapentaenoic acid Drugs 0.000 claims description 6
230000002538 fungal effect Effects 0.000 claims description 6
230000002068 genetic effect Effects 0.000 claims description 6
125000005456 glyceride group Chemical group 0.000 claims description 6
230000013595 glycosylation Effects 0.000 claims description 6
238000006206 glycosylation reaction Methods 0.000 claims description 6
230000001939 inductive effect Effects 0.000 claims description 6
210000000723 mammalian artificial chromosome Anatomy 0.000 claims description 6
235000013310 margarine Nutrition 0.000 claims description 6
239000011159 matrix material Substances 0.000 claims description 6
230000002503 metabolic effect Effects 0.000 claims description 6
239000002853 nucleic acid probe Substances 0.000 claims description 6
238000013519 translation Methods 0.000 claims description 6
239000013603 viral vector Substances 0.000 claims description 6
210000005253 yeast cell Anatomy 0.000 claims description 6
CMWTZPSULFXXJA-UHFFFAOYSA-N 2-(6-methoxy-2-naphthalenyl)propanoic acid Chemical compound C1=C(C(C)C(O)=O)C=CC2=CC(OC)=CC=C21 CMWTZPSULFXXJA-UHFFFAOYSA-N 0.000 claims description 5
235000014698 Brassica juncea var multisecta Nutrition 0.000 claims description 5
235000006008 Brassica napus var napus Nutrition 0.000 claims description 5
235000006618 Brassica rapa subsp oleifera Nutrition 0.000 claims description 5
244000061176 Nicotiana tabacum Species 0.000 claims description 5
238000012219 cassette mutagenesis Methods 0.000 claims description 5
125000004185 ester group Chemical group 0.000 claims description 5
239000000833 heterodimer Substances 0.000 claims description 5
238000001727 in vivo Methods 0.000 claims description 5
239000000543 intermediate Substances 0.000 claims description 5
239000003264 margarine Substances 0.000 claims description 5
239000008165 rice bran oil Substances 0.000 claims description 5
230000001568 sexual effect Effects 0.000 claims description 5
241001133760 Acoelorraphe Species 0.000 claims description 4
241000251468 Actinopterygii Species 0.000 claims description 4
JIGUQPWFLRLWPJ-UHFFFAOYSA-N Ethyl acrylate Chemical compound CCOC(=O)C=C JIGUQPWFLRLWPJ-UHFFFAOYSA-N 0.000 claims description 4
235000003222 Helianthus annuus Nutrition 0.000 claims description 4
BAPJBEWLBFYGME-UHFFFAOYSA-N Methyl acrylate Chemical compound COC(=O)C=C BAPJBEWLBFYGME-UHFFFAOYSA-N 0.000 claims description 4
229920000297 Rayon Polymers 0.000 claims description 4
235000019774 Rice Bran oil Nutrition 0.000 claims description 4
241000282849 Ruminantia Species 0.000 claims description 4
108700005078 Synthetic Genes Proteins 0.000 claims description 4
NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 4
125000000539 amino acid group Chemical group 0.000 claims description 4
238000007845 assembly PCR Methods 0.000 claims description 4
239000012472 biological sample Substances 0.000 claims description 4
150000001733 carboxylic acid esters Chemical class 0.000 claims description 4
238000012258 culturing Methods 0.000 claims description 4
230000002950 deficient Effects 0.000 claims description 4
235000015872 dietary supplement Nutrition 0.000 claims description 4
FKRCODPIKNYEAC-UHFFFAOYSA-N ethyl propionate Chemical compound CCOC(=O)CC FKRCODPIKNYEAC-UHFFFAOYSA-N 0.000 claims description 4
230000004907 flux Effects 0.000 claims description 4
210000004209 hair Anatomy 0.000 claims description 4
GZQKNULLWNGMCW-PWQABINMSA-N lipid A (E. coli) Chemical compound O1[C@H](CO)[C@@H](OP(O)(O)=O)[C@H](OC(=O)C[C@@H](CCCCCCCCCCC)OC(=O)CCCCCCCCCCCCC)[C@@H](NC(=O)C[C@@H](CCCCCCCCCCC)OC(=O)CCCCCCCCCCC)[C@@H]1OC[C@@H]1[C@@H](O)[C@H](OC(=O)C[C@H](O)CCCCCCCCCCC)[C@@H](NC(=O)C[C@H](O)CCCCCCCCCCC)[C@@H](OP(O)(O)=O)O1 GZQKNULLWNGMCW-PWQABINMSA-N 0.000 claims description 4
229920006008 lipopolysaccharide Polymers 0.000 claims description 4
230000014759 maintenance of location Effects 0.000 claims description 4
229910052751 metal Inorganic materials 0.000 claims description 4
239000002184 metal Substances 0.000 claims description 4
230000005012 migration Effects 0.000 claims description 4
238000013508 migration Methods 0.000 claims description 4
235000013336 milk Nutrition 0.000 claims description 4
239000008267 milk Substances 0.000 claims description 4
210000004080 milk Anatomy 0.000 claims description 4
230000033607 mismatch repair Effects 0.000 claims description 4
239000000178 monomer Substances 0.000 claims description 4
235000016709 nutrition Nutrition 0.000 claims description 4
239000002245 particle Substances 0.000 claims description 4
PNJWIWWMYCMZRO-UHFFFAOYSA-N pent‐4‐en‐2‐one Natural products CC(=O)CC=C PNJWIWWMYCMZRO-UHFFFAOYSA-N 0.000 claims description 4
229920000642 polymer Polymers 0.000 claims description 4
102000054765 polymorphisms of proteins Human genes 0.000 claims description 4
239000011541 reaction mixture Substances 0.000 claims description 4
238000007670 refining Methods 0.000 claims description 4
230000008439 repair process Effects 0.000 claims description 4
239000011347 resin Substances 0.000 claims description 4
229920005989 resin Polymers 0.000 claims description 4
239000007787 solid Substances 0.000 claims description 4
230000000707 stereoselective effect Effects 0.000 claims description 4
230000001131 transforming effect Effects 0.000 claims description 4
238000005406 washing Methods 0.000 claims description 4
DVSZKTAMJJTWFG-SKCDLICFSA-N (2e,4e,6e,8e,10e,12e)-docosa-2,4,6,8,10,12-hexaenoic acid Chemical compound CCCCCCCCC\C=C\C=C\C=C\C=C\C=C\C=C\C(O)=O DVSZKTAMJJTWFG-SKCDLICFSA-N 0.000 claims description 3
AQIHDXGKQHFBNW-ZCFIWIBFSA-N (2r)-2-(4-hydroxyphenoxy)propanoic acid Chemical compound OC(=O)[C@@H](C)OC1=CC=C(O)C=C1 AQIHDXGKQHFBNW-ZCFIWIBFSA-N 0.000 claims description 3
GZJLLYHBALOKEX-UHFFFAOYSA-N 6-Ketone, O18-Me-Ussuriedine Natural products CC=CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O GZJLLYHBALOKEX-UHFFFAOYSA-N 0.000 claims description 3
235000017060 Arachis glabrata Nutrition 0.000 claims description 3
244000105624 Arachis hypogaea Species 0.000 claims description 3
235000010777 Arachis hypogaea Nutrition 0.000 claims description 3
235000018262 Arachis monticola Nutrition 0.000 claims description 3
240000007817 Olea europaea Species 0.000 claims description 3
235000003434 Sesamum indicum Nutrition 0.000 claims description 3
244000040738 Sesamum orientale Species 0.000 claims description 3
238000006136 alcoholysis reaction Methods 0.000 claims description 3
239000010775 animal oil Substances 0.000 claims description 3
235000021342 arachidonic acid Nutrition 0.000 claims description 3
229940114079 arachidonic acid Drugs 0.000 claims description 3
210000004507 artificial chromosome Anatomy 0.000 claims description 3
238000004113 cell culture Methods 0.000 claims description 3
230000003247 decreasing effect Effects 0.000 claims description 3
235000020669 docosahexaenoic acid Nutrition 0.000 claims description 3
KAUVQQXNCKESLC-UHFFFAOYSA-N docosahexaenoic acid (DHA) Natural products COC(=O)C(C)NOCC1=CC=CC=C1 KAUVQQXNCKESLC-UHFFFAOYSA-N 0.000 claims description 3
239000000834 fixative Substances 0.000 claims description 3
239000002778 food additive Substances 0.000 claims description 3
230000028996 humoral immune response Effects 0.000 claims description 3
239000010720 hydraulic oil Substances 0.000 claims description 3
DKYWVDODHFEZIM-UHFFFAOYSA-N ketoprofen Chemical compound OC(=O)C(C)C1=CC=CC(C(=O)C=2C=CC=CC=2)=C1 DKYWVDODHFEZIM-UHFFFAOYSA-N 0.000 claims description 3
229960000991 ketoprofen Drugs 0.000 claims description 3
239000002502 liposome Substances 0.000 claims description 3
235000020978 long-chain polyunsaturated fatty acids Nutrition 0.000 claims description 3
231100000219 mutagenic Toxicity 0.000 claims description 3
230000003505 mutagenic effect Effects 0.000 claims description 3
230000002018 overexpression Effects 0.000 claims description 3
230000036961 partial effect Effects 0.000 claims description 3
235000020232 peanut Nutrition 0.000 claims description 3
239000008188 pellet Substances 0.000 claims description 3
239000008194 pharmaceutical composition Substances 0.000 claims description 3
239000000843 powder Substances 0.000 claims description 3
210000001938 protoplast Anatomy 0.000 claims description 3
239000002002 slurry Substances 0.000 claims description 3
241001515965 unidentified phage Species 0.000 claims description 3
229940035893 uracil Drugs 0.000 claims description 3
SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 claims description 2
JAHNSTQSQJOJLO-UHFFFAOYSA-N 2-(3-fluorophenyl)-1h-imidazole Chemical compound FC1=CC=CC(C=2NC=CN=2)=C1 JAHNSTQSQJOJLO-UHFFFAOYSA-N 0.000 claims description 2
240000008564 Boehmeria nivea Species 0.000 claims description 2
108700010070 Codon Usage Proteins 0.000 claims description 2
230000004544 DNA amplification Effects 0.000 claims description 2
241001057636 Dracaena deremensis Species 0.000 claims description 2
241000702191 Escherichia virus P1 Species 0.000 claims description 2
235000004431 Linum usitatissimum Nutrition 0.000 claims description 2
240000006240 Linum usitatissimum Species 0.000 claims description 2
229920000433 Lyocell Polymers 0.000 claims description 2
CERQOIWHTDAKMF-UHFFFAOYSA-N Methacrylic acid Chemical compound CC(=C)C(O)=O CERQOIWHTDAKMF-UHFFFAOYSA-N 0.000 claims description 2
230000004988 N-glycosylation Effects 0.000 claims description 2
239000004952 Polyamide Substances 0.000 claims description 2
230000006578 abscission Effects 0.000 claims description 2
238000010521 absorption reaction Methods 0.000 claims description 2
150000001252 acrylic acid derivatives Chemical class 0.000 claims description 2
229920006397 acrylic thermoplastic Polymers 0.000 claims description 2
239000012190 activator Substances 0.000 claims description 2
239000000443 aerosol Substances 0.000 claims description 2
239000011324 bead Substances 0.000 claims description 2
239000003833 bile salt Substances 0.000 claims description 2
230000001851 biosynthetic effect Effects 0.000 claims description 2
CQEYYJKEWSMYFG-UHFFFAOYSA-N butyl acrylate Chemical compound CCCCOC(=O)C=C CQEYYJKEWSMYFG-UHFFFAOYSA-N 0.000 claims description 2
239000000919 ceramic Substances 0.000 claims description 2
230000008859 change Effects 0.000 claims description 2
235000020247 cow milk Nutrition 0.000 claims description 2
LDHQCZJRKDOVOX-NSCUHMNNSA-N crotonic acid Chemical compound C\C=C\C(O)=O LDHQCZJRKDOVOX-NSCUHMNNSA-N 0.000 claims description 2
230000009849 deactivation Effects 0.000 claims description 2
238000004925 denaturation Methods 0.000 claims description 2
230000036425 denaturation Effects 0.000 claims description 2
238000004043 dyeing Methods 0.000 claims description 2
238000004520 electroporation Methods 0.000 claims description 2
238000001914 filtration Methods 0.000 claims description 2
239000011521 glass Substances 0.000 claims description 2
230000001279 glycosylating effect Effects 0.000 claims description 2
230000012010 growth Effects 0.000 claims description 2
210000004408 hybridoma Anatomy 0.000 claims description 2
230000006872 improvement Effects 0.000 claims description 2
239000003112 inhibitor Substances 0.000 claims description 2
239000003456 ion exchange resin Substances 0.000 claims description 2
229920003303 ion-exchange polymer Polymers 0.000 claims description 2
150000004668 long chain fatty acids Chemical class 0.000 claims description 2
238000005259 measurement Methods 0.000 claims description 2
230000001404 mediated effect Effects 0.000 claims description 2
LVHBHZANLOWSRM-UHFFFAOYSA-N methylenebutanedioic acid Natural products OC(=O)CC(=C)C(O)=O LVHBHZANLOWSRM-UHFFFAOYSA-N 0.000 claims description 2
238000000520 microinjection Methods 0.000 claims description 2
238000012544 monitoring process Methods 0.000 claims description 2
229920003229 poly(methyl methacrylate) Polymers 0.000 claims description 2
229920002647 polyamide Polymers 0.000 claims description 2
229920000728 polyester Polymers 0.000 claims description 2
230000001737 promoting effect Effects 0.000 claims description 2
PNXMTCDJUBJHQJ-UHFFFAOYSA-N propyl prop-2-enoate Chemical compound CCCOC(=O)C=C PNXMTCDJUBJHQJ-UHFFFAOYSA-N 0.000 claims description 2
239000002964 rayon Substances 0.000 claims description 2
230000001177 retroviral effect Effects 0.000 claims description 2
239000002689 soil Substances 0.000 claims description 2
235000020238 sunflower seed Nutrition 0.000 claims description 2
239000004094 surface-active agent Substances 0.000 claims description 2
ISXSCDLOGDJUNJ-UHFFFAOYSA-N tert-butyl prop-2-enoate Chemical compound CC(C)(C)OC(=O)C=C ISXSCDLOGDJUNJ-UHFFFAOYSA-N 0.000 claims description 2
231100000419 toxicity Toxicity 0.000 claims description 2
230000001988 toxicity Effects 0.000 claims description 2
LDHQCZJRKDOVOX-UHFFFAOYSA-N trans-crotonic acid Natural products CC=CC(O)=O LDHQCZJRKDOVOX-UHFFFAOYSA-N 0.000 claims description 2
241000701161 unidentified adenovirus Species 0.000 claims description 2
235000013311 vegetables Nutrition 0.000 claims description 2
125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 claims description 2
229920002554 vinyl polymer Polymers 0.000 claims description 2
210000002268 wool Anatomy 0.000 claims description 2
150000002759 monoacylglycerols Chemical class 0.000 claims 28
108091030071 RNAI Proteins 0.000 claims 7
240000007594 Oryza sativa Species 0.000 claims 6
240000005979 Hordeum vulgare Species 0.000 claims 5
241000589155 Agrobacterium tumefaciens Species 0.000 claims 2
125000001531 monoacylglycerol group Chemical group 0.000 claims 2
125000003203 triacylglycerol group Chemical group 0.000 claims 2
244000188595 Brassica sinapistrum Species 0.000 claims 1
244000025254 Cannabis sativa Species 0.000 claims 1
244000020551 Helianthus annuus Species 0.000 claims 1
240000003768 Solanum lycopersicum Species 0.000 claims 1
240000003829 Sorghum propinquum Species 0.000 claims 1
125000005250 alkyl acrylate group Chemical group 0.000 claims 1
239000007891 compressed tablet Substances 0.000 claims 1
235000013350 formula milk Nutrition 0.000 claims 1
239000000710 homodimer Substances 0.000 claims 1
239000000017 hydrogel Substances 0.000 claims 1
235000021374 legumes Nutrition 0.000 claims 1
108700023418 Amidases Proteins 0.000 abstract description 20
102000005922 amidase Human genes 0.000 abstract description 20
101710091688 Patatin Proteins 0.000 abstract description 10
239000002537 cosmetic Substances 0.000 abstract description 6
239000002417 nutraceutical Substances 0.000 abstract description 5
235000021436 nutraceutical agent Nutrition 0.000 abstract description 5
229940088598 enzyme Drugs 0.000 description 57
102000035195 Peptidases Human genes 0.000 description 43
229940040461 lipase Drugs 0.000 description 35
235000019419 proteases Nutrition 0.000 description 34
235000018102 proteins Nutrition 0.000 description 27
235000001014 amino acid Nutrition 0.000 description 22
229940024606 amino acid Drugs 0.000 description 20
239000003925 fat Substances 0.000 description 19
239000002585 base Substances 0.000 description 16
150000003904 phospholipids Chemical class 0.000 description 15
230000008569 process Effects 0.000 description 14
238000013518 transcription Methods 0.000 description 13
230000035897 transcription Effects 0.000 description 13
102000014384 Type C Phospholipases Human genes 0.000 description 12
108010079194 Type C Phospholipases Proteins 0.000 description 12
125000003275 alpha amino acid group Chemical group 0.000 description 11
102100037883 Phospholipase B1, membrane-associated Human genes 0.000 description 10
235000021323 fish oil Nutrition 0.000 description 10
239000013615 primer Substances 0.000 description 10
108091032973 (ribonucleotides)n+m Proteins 0.000 description 9
239000003814 drug Substances 0.000 description 9
108010058864 Phospholipases A2 Proteins 0.000 description 8
150000003905 phosphatidylinositols Chemical class 0.000 description 8
239000002243 precursor Substances 0.000 description 8
241000209094 Oryza Species 0.000 description 7
108090000553 Phospholipase D Proteins 0.000 description 7
102000011420 Phospholipase D Human genes 0.000 description 7
235000011187 glycerol Nutrition 0.000 description 7
238000006467 substitution reaction Methods 0.000 description 7
WTJKGGKOPKCXLL-RRHRGVEJSA-N phosphatidylcholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCC=CCCCCCCCC WTJKGGKOPKCXLL-RRHRGVEJSA-N 0.000 description 6
150000008104 phosphatidylethanolamines Chemical class 0.000 description 6
238000012545 processing Methods 0.000 description 6
TZCPCKNHXULUIY-RGULYWFUSA-N 1,2-distearoyl-sn-glycero-3-phosphoserine Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC[C@H](COP(O)(=O)OC[C@H](N)C(O)=O)OC(=O)CCCCCCCCCCCCCCCCC TZCPCKNHXULUIY-RGULYWFUSA-N 0.000 description 5
CKLJMWTZIZZHCS-UWTATZPHSA-N D-aspartic acid Chemical compound OC(=O)[C@H](N)CC(O)=O CKLJMWTZIZZHCS-UWTATZPHSA-N 0.000 description 5
JZNWSCPGTDBMEW-UHFFFAOYSA-N Glycerophosphorylethanolamin Natural products NCCOP(O)(=O)OCC(O)CO JZNWSCPGTDBMEW-UHFFFAOYSA-N 0.000 description 5
ZWZWYGMENQVNFU-UHFFFAOYSA-N Glycerophosphorylserin Natural products OC(=O)C(N)COP(O)(=O)OCC(O)CO ZWZWYGMENQVNFU-UHFFFAOYSA-N 0.000 description 5
238000012228 RNA interference-mediated gene silencing Methods 0.000 description 5
108020004459 Small interfering RNA Proteins 0.000 description 5
230000001105 regulatory effect Effects 0.000 description 5
PORPENFLTBBHSG-MGBGTMOVSA-N 1,2-dihexadecanoyl-sn-glycerol-3-phosphate Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP(O)(O)=O)OC(=O)CCCCCCCCCCCCCCC PORPENFLTBBHSG-MGBGTMOVSA-N 0.000 description 4
240000000385 Brassica napus var. napus Species 0.000 description 4
ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 4
241000209219 Hordeum Species 0.000 description 4
235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 4
238000003556 assay Methods 0.000 description 4
238000010586 diagram Methods 0.000 description 4
239000003623 enhancer Substances 0.000 description 4
IPCSVZSSVZVIGE-UHFFFAOYSA-M hexadecanoate Chemical compound CCCCCCCCCCCCCCCC([O-])=O IPCSVZSSVZVIGE-UHFFFAOYSA-M 0.000 description 4
235000009973 maize Nutrition 0.000 description 4
239000012528 membrane Substances 0.000 description 4
230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 4
230000014616 translation Effects 0.000 description 4
239000002699 waste material Substances 0.000 description 4
241000894006 Bacteria Species 0.000 description 3
241000701489 Cauliflower mosaic virus Species 0.000 description 3
241000208818 Helianthus Species 0.000 description 3
108091092195 Intron Proteins 0.000 description 3
241000208125 Nicotiana Species 0.000 description 3
229910019142 PO4 Inorganic materials 0.000 description 3
101000942681 Rattus norvegicus Clusterin Proteins 0.000 description 3
230000000692 anti-sense effect Effects 0.000 description 3
230000004071 biological effect Effects 0.000 description 3
239000000828 canola oil Substances 0.000 description 3
239000002775 capsule Substances 0.000 description 3
239000003795 chemical substances by application Substances 0.000 description 3
238000003776 cleavage reaction Methods 0.000 description 3
239000002299 complementary DNA Substances 0.000 description 3
239000002285 corn oil Substances 0.000 description 3
235000019688 fish Nutrition 0.000 description 3
150000002596 lactones Chemical group 0.000 description 3
108091070501 miRNA Proteins 0.000 description 3
239000002679 microRNA Substances 0.000 description 3
150000007524 organic acids Chemical class 0.000 description 3
235000005985 organic acids Nutrition 0.000 description 3
239000000575 pesticide Substances 0.000 description 3
239000010452 phosphate Substances 0.000 description 3
235000021317 phosphate Nutrition 0.000 description 3
150000003839 salts Chemical class 0.000 description 3
230000007017 scission Effects 0.000 description 3
239000003549 soybean oil Substances 0.000 description 3
239000003826 tablet Substances 0.000 description 3
230000003612 virological effect Effects 0.000 description 3
JTTHKOPSMAVJFE-SECBINFHSA-N (2r)-2-azaniumyl-4-phenylbutanoate Chemical compound [O-]C(=O)[C@H]([NH3+])CCC1=CC=CC=C1 JTTHKOPSMAVJFE-SECBINFHSA-N 0.000 description 2
ODIGIKRIUKFKHP-UHFFFAOYSA-N (n-propan-2-yloxycarbonylanilino) acetate Chemical compound CC(C)OC(=O)N(OC(C)=O)C1=CC=CC=C1 ODIGIKRIUKFKHP-UHFFFAOYSA-N 0.000 description 2
241000219194 Arabidopsis Species 0.000 description 2
101001065065 Aspergillus awamori Feruloyl esterase A Proteins 0.000 description 2
241001515826 Cassava vein mosaic virus Species 0.000 description 2
108700024394 Exon Proteins 0.000 description 2
102000003886 Glycoproteins Human genes 0.000 description 2
108090000288 Glycoproteins Proteins 0.000 description 2
108020002496 Lysophospholipase Proteins 0.000 description 2
241000124008 Mammalia Species 0.000 description 2
101000969137 Mus musculus Metallothionein-1 Proteins 0.000 description 2
102000004316 Oxidoreductases Human genes 0.000 description 2
108090000854 Oxidoreductases Proteins 0.000 description 2
108091093037 Peptide nucleic acid Proteins 0.000 description 2
108010069394 Phosphatidate Phosphatase Proteins 0.000 description 2
102000001107 Phosphatidate Phosphatase Human genes 0.000 description 2
102000006486 Phosphoinositide Phospholipase C Human genes 0.000 description 2
108010044302 Phosphoinositide phospholipase C Proteins 0.000 description 2
NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
108010059820 Polygalacturonase Proteins 0.000 description 2
241000701507 Rice tungro bacilliform virus Species 0.000 description 2
235000019486 Sunflower oil Nutrition 0.000 description 2
230000002378 acidificating effect Effects 0.000 description 2
239000008346 aqueous phase Substances 0.000 description 2
239000007864 aqueous solution Substances 0.000 description 2
238000003491 array Methods 0.000 description 2
235000003704 aspartic acid Nutrition 0.000 description 2
OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 2
150000001732 carboxylic acid derivatives Chemical class 0.000 description 2
230000015556 catabolic process Effects 0.000 description 2
238000006555 catalytic reaction Methods 0.000 description 2
230000024245 cell differentiation Effects 0.000 description 2
235000013351 cheese Nutrition 0.000 description 2
235000019877 cocoa butter equivalent Nutrition 0.000 description 2
238000010276 construction Methods 0.000 description 2
239000006071 cream Substances 0.000 description 2
239000013078 crystal Substances 0.000 description 2
238000006731 degradation reaction Methods 0.000 description 2
238000001514 detection method Methods 0.000 description 2
238000011161 development Methods 0.000 description 2
230000018109 developmental process Effects 0.000 description 2
230000001079 digestive effect Effects 0.000 description 2
238000009826 distribution Methods 0.000 description 2
239000003995 emulsifying agent Substances 0.000 description 2
230000002255 enzymatic effect Effects 0.000 description 2
238000001952 enzyme assay Methods 0.000 description 2
230000006870 function Effects 0.000 description 2
125000000487 histidyl group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 description 2
238000003780 insertion Methods 0.000 description 2
230000037431 insertion Effects 0.000 description 2
238000002372 labelling Methods 0.000 description 2
101150066555 lacZ gene Proteins 0.000 description 2
239000002960 lipid emulsion Substances 0.000 description 2
108020004999 messenger RNA Proteins 0.000 description 2
230000004060 metabolic process Effects 0.000 description 2
TWXDDNPPQUTEOV-FVGYRXGTSA-N methamphetamine hydrochloride Chemical compound Cl.CN[C@@H](C)CC1=CC=CC=C1 TWXDDNPPQUTEOV-FVGYRXGTSA-N 0.000 description 2
235000014593 oils and fats Nutrition 0.000 description 2
229940049964 oleate Drugs 0.000 description 2
ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 2
238000005580 one pot reaction Methods 0.000 description 2
210000000056 organ Anatomy 0.000 description 2
239000003960 organic solvent Substances 0.000 description 2
238000005192 partition Methods 0.000 description 2
239000012071 phase Substances 0.000 description 2
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 2
239000006187 pill Substances 0.000 description 2
235000019833 protease Nutrition 0.000 description 2
239000000243 solution Substances 0.000 description 2
239000007858 starting material Substances 0.000 description 2
239000002600 sunflower oil Substances 0.000 description 2
239000004753 textile Substances 0.000 description 2
230000005026 transcription initiation Effects 0.000 description 2
230000002103 transcriptional effect Effects 0.000 description 2
241001430294 unidentified retrovirus Species 0.000 description 2
GZCWLCBFPRFLKL-UHFFFAOYSA-N 1-prop-2-ynoxypropan-2-ol Chemical compound CC(O)COCC#C GZCWLCBFPRFLKL-UHFFFAOYSA-N 0.000 description 1
LRYZPFWEZHSTHD-HEFFAWAOSA-O 2-[[(e,2s,3r)-2-formamido-3-hydroxyoctadec-4-enoxy]-hydroxyphosphoryl]oxyethyl-trimethylazanium Chemical class CCCCCCCCCCCCC\C=C\[C@@H](O)[C@@H](NC=O)COP(O)(=O)OCC[N+](C)(C)C LRYZPFWEZHSTHD-HEFFAWAOSA-O 0.000 description 1
OSJPPGNTCRNQQC-UWTATZPHSA-N 3-phospho-D-glyceric acid Chemical compound OC(=O)[C@H](O)COP(O)(O)=O OSJPPGNTCRNQQC-UWTATZPHSA-N 0.000 description 1
101000818108 Acholeplasma phage L2 Uncharacterized 81.3 kDa protein Proteins 0.000 description 1
108010051457 Acid Phosphatase Proteins 0.000 description 1
102000013563 Acid Phosphatase Human genes 0.000 description 1
NIXOWILDQLNWCW-UHFFFAOYSA-M Acrylate Chemical compound [O-]C(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-M 0.000 description 1
241000589158 Agrobacterium Species 0.000 description 1
102000004400 Aminopeptidases Human genes 0.000 description 1
108090000915 Aminopeptidases Proteins 0.000 description 1
244000144725 Amygdalus communis Species 0.000 description 1
108010065511 Amylases Proteins 0.000 description 1
102000013142 Amylases Human genes 0.000 description 1
108700040922 Arabidopsis LFY Proteins 0.000 description 1
101710152845 Arabinogalactan endo-beta-1,4-galactanase Proteins 0.000 description 1
239000004475 Arginine Substances 0.000 description 1
DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
102000035101 Aspartic proteases Human genes 0.000 description 1
108091005502 Aspartic proteases Proteins 0.000 description 1
108700038091 Beta-glucanases Proteins 0.000 description 1
102100032487 Beta-mannosidase Human genes 0.000 description 1
239000002028 Biomass Substances 0.000 description 1
241000283690 Bos taurus Species 0.000 description 1
235000011293 Brassica napus Nutrition 0.000 description 1
FERIUCNNQQJTOY-UHFFFAOYSA-M Butyrate Chemical compound CCCC([O-])=O FERIUCNNQQJTOY-UHFFFAOYSA-M 0.000 description 1
FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Natural products CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 1
102000005367 Carboxypeptidases Human genes 0.000 description 1
108010006303 Carboxypeptidases Proteins 0.000 description 1
206010007559 Cardiac failure congestive Diseases 0.000 description 1
102000011632 Caseins Human genes 0.000 description 1
108010076119 Caseins Proteins 0.000 description 1
108010084185 Cellulases Proteins 0.000 description 1
102000005575 Cellulases Human genes 0.000 description 1
108010008885 Cellulose 1,4-beta-Cellobiosidase Proteins 0.000 description 1
108090000317 Chymotrypsin Proteins 0.000 description 1
241000218631 Coniferophyta Species 0.000 description 1
108010005843 Cysteine Proteases Proteins 0.000 description 1
102000005927 Cysteine Proteases Human genes 0.000 description 1
108090000695 Cytokines Proteins 0.000 description 1
102000004127 Cytokines Human genes 0.000 description 1
150000008574 D-amino acids Chemical class 0.000 description 1
239000003155 DNA primer Substances 0.000 description 1
108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
102000003779 Dipeptidyl-peptidases and tripeptidyl-peptidases Human genes 0.000 description 1
108090000194 Dipeptidyl-peptidases and tripeptidyl-peptidases Proteins 0.000 description 1
108010061435 Enalapril Proteins 0.000 description 1
101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 1
101710147028 Endo-beta-1,4-galactanase Proteins 0.000 description 1
101710111935 Endo-beta-1,4-glucanase Proteins 0.000 description 1
108010013369 Enteropeptidase Proteins 0.000 description 1
102100029727 Enteropeptidase Human genes 0.000 description 1
241000588724 Escherichia coli Species 0.000 description 1
108091029865 Exogenous DNA Proteins 0.000 description 1
102000018389 Exopeptidases Human genes 0.000 description 1
108010091443 Exopeptidases Proteins 0.000 description 1
108010074860 Factor Xa Proteins 0.000 description 1
108010010803 Gelatin Proteins 0.000 description 1
108700039691 Genetic Promoter Regions Proteins 0.000 description 1
102100022624 Glucoamylase Human genes 0.000 description 1
108050008938 Glucoamylases Proteins 0.000 description 1
WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
UXDDRFCJKNROTO-UHFFFAOYSA-N Glycerol 1,2-diacetate Chemical compound CC(=O)OCC(CO)OC(C)=O UXDDRFCJKNROTO-UHFFFAOYSA-N 0.000 description 1
241000219146 Gossypium Species 0.000 description 1
240000002024 Gossypium herbaceum Species 0.000 description 1
235000004341 Gossypium herbaceum Nutrition 0.000 description 1
101150094780 Gpc2 gene Proteins 0.000 description 1
101000912350 Haemophilus phage HP1 (strain HP1c1) DNA N-6-adenine-methyltransferase Proteins 0.000 description 1
206010019280 Heart failures Diseases 0.000 description 1
241000735445 Hetrodes Species 0.000 description 1
101001096022 Homo sapiens Phospholipase B1, membrane-associated Proteins 0.000 description 1
206010020772 Hypertension Diseases 0.000 description 1
208000001953 Hypotension Diseases 0.000 description 1
108060003951 Immunoglobulin Proteins 0.000 description 1
108060005987 Kallikrein Proteins 0.000 description 1
102000001399 Kallikrein Human genes 0.000 description 1
241000235058 Komagataella pastoris Species 0.000 description 1
DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
108010029541 Laccase Proteins 0.000 description 1
108091026898 Leader sequence (mRNA) Proteins 0.000 description 1
ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
108010007859 Lisinopril Proteins 0.000 description 1
241000227653 Lycopersicon Species 0.000 description 1
KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
239000004472 Lysine Substances 0.000 description 1
101710170142 Lysophospholipase 2 Proteins 0.000 description 1
241000218922 Magnoliophyta Species 0.000 description 1
102000005741 Metalloproteases Human genes 0.000 description 1
108010006035 Metalloproteases Proteins 0.000 description 1
108700011259 MicroRNAs Proteins 0.000 description 1
241000699666 Mus <mouse, genus> Species 0.000 description 1
108091061960 Naked DNA Proteins 0.000 description 1
108091005461 Nucleic proteins Proteins 0.000 description 1
108010038807 Oligopeptides Proteins 0.000 description 1
102000015636 Oligopeptides Human genes 0.000 description 1
108700026244 Open Reading Frames Proteins 0.000 description 1
235000011464 Pachycereus pringlei Nutrition 0.000 description 1
235000011466 Pachycereus weberi Nutrition 0.000 description 1
240000006939 Pachycereus weberi Species 0.000 description 1
235000019482 Palm oil Nutrition 0.000 description 1
108010067372 Pancreatic elastase Proteins 0.000 description 1
102000016387 Pancreatic elastase Human genes 0.000 description 1
108010029182 Pectin lyase Proteins 0.000 description 1
108700020962 Peroxidase Proteins 0.000 description 1
102000003992 Peroxidases Human genes 0.000 description 1
102100032983 Phospholipase D2 Human genes 0.000 description 1
OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
108091000080 Phosphotransferase Proteins 0.000 description 1
108700001094 Plant Genes Proteins 0.000 description 1
208000020584 Polyploidy Diseases 0.000 description 1
101710118538 Protease Proteins 0.000 description 1
230000006819 RNA synthesis Effects 0.000 description 1
108020004511 Recombinant DNA Proteins 0.000 description 1
108091007187 Reductases Proteins 0.000 description 1
108700008625 Reporter Genes Proteins 0.000 description 1
102000004389 Ribonucleoproteins Human genes 0.000 description 1
108010081734 Ribonucleoproteins Proteins 0.000 description 1
101150090744 SPL3 gene Proteins 0.000 description 1
240000004808 Saccharomyces cerevisiae Species 0.000 description 1
206010040047 Sepsis Diseases 0.000 description 1
102000012479 Serine Proteases Human genes 0.000 description 1
108010022999 Serine Proteases Proteins 0.000 description 1
BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
101150019148 Slc7a3 gene Proteins 0.000 description 1
229920002472 Starch Polymers 0.000 description 1
108091027544 Subgenomic mRNA Proteins 0.000 description 1
108090000787 Subtilisin Proteins 0.000 description 1
206010042602 Supraventricular extrasystoles Diseases 0.000 description 1
102000002933 Thioredoxin Human genes 0.000 description 1
108091036066 Three prime untranslated region Proteins 0.000 description 1
102000006601 Thymidine Kinase Human genes 0.000 description 1
108020004440 Thymidine kinase Proteins 0.000 description 1
241000723848 Tobamovirus Species 0.000 description 1
108091023040 Transcription factor Proteins 0.000 description 1
102000040945 Transcription factor Human genes 0.000 description 1
108020004566 Transfer RNA Proteins 0.000 description 1
108060008539 Transglutaminase Proteins 0.000 description 1
108090000631 Trypsin Proteins 0.000 description 1
102000004142 Trypsin Human genes 0.000 description 1
102000003425 Tyrosinase Human genes 0.000 description 1
108060008724 Tyrosinase Proteins 0.000 description 1
KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
108010036419 acyl-(acyl-carrier-protein)desaturase Proteins 0.000 description 1
239000000654 additive Substances 0.000 description 1
238000001261 affinity purification Methods 0.000 description 1
108090000637 alpha-Amylases Proteins 0.000 description 1
229910000147 aluminium phosphate Inorganic materials 0.000 description 1
238000007098 aminolysis reaction Methods 0.000 description 1
235000019418 amylase Nutrition 0.000 description 1
229940025131 amylases Drugs 0.000 description 1
235000019730 animal feed additive Nutrition 0.000 description 1
239000002260 anti-inflammatory agent Substances 0.000 description 1
230000003110 anti-inflammatory effect Effects 0.000 description 1
230000001741 anti-phlogistic effect Effects 0.000 description 1
239000004599 antimicrobial Substances 0.000 description 1
ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
125000000637 arginyl group Chemical class N[C@@H](CCCNC(N)=N)C(=O)* 0.000 description 1
235000009582 asparagine Nutrition 0.000 description 1
229960001230 asparagine Drugs 0.000 description 1
210000003719 b-lymphocyte Anatomy 0.000 description 1
OGBUMNBNEWYMNJ-UHFFFAOYSA-N batilol Chemical class CCCCCCCCCCCCCCCCCCOCC(O)CO OGBUMNBNEWYMNJ-UHFFFAOYSA-N 0.000 description 1
230000008901 benefit Effects 0.000 description 1
108010055059 beta-Mannosidase Proteins 0.000 description 1
235000013361 beverage Nutrition 0.000 description 1
230000033228 biological regulation Effects 0.000 description 1
210000000988 bone and bone Anatomy 0.000 description 1
235000014121 butter Nutrition 0.000 description 1
235000019519 canola oil Nutrition 0.000 description 1
150000001720 carbohydrates Chemical class 0.000 description 1
235000014633 carbohydrates Nutrition 0.000 description 1
229910052799 carbon Inorganic materials 0.000 description 1
239000005018 casein Substances 0.000 description 1
BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
235000021240 caseins Nutrition 0.000 description 1
239000003054 catalyst Substances 0.000 description 1
230000003197 catalytic effect Effects 0.000 description 1
230000003915 cell function Effects 0.000 description 1
210000000170 cell membrane Anatomy 0.000 description 1
230000001413 cellular effect Effects 0.000 description 1
230000005754 cellular signaling Effects 0.000 description 1
238000005119 centrifugation Methods 0.000 description 1
235000013339 cereals Nutrition 0.000 description 1
238000010028 chemical finishing Methods 0.000 description 1
239000003153 chemical reaction reagent Substances 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
230000002759 chromosomal effect Effects 0.000 description 1
210000000349 chromosome Anatomy 0.000 description 1
229960002376 chymotrypsin Drugs 0.000 description 1
230000001010 compromised effect Effects 0.000 description 1
108091036078 conserved sequence Proteins 0.000 description 1
230000001276 controlling effect Effects 0.000 description 1
235000005687 corn oil Nutrition 0.000 description 1
108010005400 cutinase Proteins 0.000 description 1
238000005520 cutting process Methods 0.000 description 1
235000013365 dairy product Nutrition 0.000 description 1
230000007812 deficiency Effects 0.000 description 1
229940124447 delivery agent Drugs 0.000 description 1
210000003298 dental enamel Anatomy 0.000 description 1
239000000645 desinfectant Substances 0.000 description 1
235000005911 diet Nutrition 0.000 description 1
230000000378 dietary effect Effects 0.000 description 1
235000004879 dioscorea Nutrition 0.000 description 1
238000004821 distillation Methods 0.000 description 1
POULHZVOKOAJMA-UHFFFAOYSA-M dodecanoate Chemical compound CCCCCCCCCCCC([O-])=O POULHZVOKOAJMA-UHFFFAOYSA-M 0.000 description 1
229940079593 drug Drugs 0.000 description 1
238000012377 drug delivery Methods 0.000 description 1
235000013399 edible fruits Nutrition 0.000 description 1
239000000839 emulsion Substances 0.000 description 1
GBXSMTUPTTWBMN-XIRDDKMYSA-N enalapril Chemical compound C([C@@H](C(=O)OCC)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(O)=O)CC1=CC=CC=C1 GBXSMTUPTTWBMN-XIRDDKMYSA-N 0.000 description 1
229960000873 enalapril Drugs 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
239000002532 enzyme inhibitor Substances 0.000 description 1
210000003527 eukaryotic cell Anatomy 0.000 description 1
108010093305 exopolygalacturonase Proteins 0.000 description 1
239000013613 expression plasmid Substances 0.000 description 1
238000000605 extraction Methods 0.000 description 1
235000019211 fat replacer Nutrition 0.000 description 1
239000010685 fatty oil Substances 0.000 description 1
230000035558 fertility Effects 0.000 description 1
239000000796 flavoring agent Substances 0.000 description 1
235000019634 flavors Nutrition 0.000 description 1
235000013373 food additive Nutrition 0.000 description 1
235000003599 food sweetener Nutrition 0.000 description 1
238000009472 formulation Methods 0.000 description 1
229920000159 gelatin Polymers 0.000 description 1
239000008273 gelatin Substances 0.000 description 1
235000019322 gelatine Nutrition 0.000 description 1
235000011852 gelatine desserts Nutrition 0.000 description 1
235000013922 glutamic acid Nutrition 0.000 description 1
239000004220 glutamic acid Substances 0.000 description 1
ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
150000002314 glycerols Chemical class 0.000 description 1
230000002414 glycolytic effect Effects 0.000 description 1
239000008187 granular material Substances 0.000 description 1
108010002430 hemicellulase Proteins 0.000 description 1
239000005556 hormone Substances 0.000 description 1
229940088597 hormone Drugs 0.000 description 1
210000000688 human artificial chromosome Anatomy 0.000 description 1
239000010903 husk Substances 0.000 description 1
230000002209 hydrophobic effect Effects 0.000 description 1
230000036543 hypotension Effects 0.000 description 1
230000028993 immune response Effects 0.000 description 1
102000018358 immunoglobulin Human genes 0.000 description 1
230000001976 improved effect Effects 0.000 description 1
238000000338 in vitro Methods 0.000 description 1
230000005764 inhibitory process Effects 0.000 description 1
239000002917 insecticide Substances 0.000 description 1
230000010354 integration Effects 0.000 description 1
230000002452 interceptive effect Effects 0.000 description 1
230000003834 intracellular effect Effects 0.000 description 1
AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
229960000310 isoleucine Drugs 0.000 description 1
238000006317 isomerization reaction Methods 0.000 description 1
238000011005 laboratory method Methods 0.000 description 1
101150109249 lacI gene Proteins 0.000 description 1
229940070765 laurate Drugs 0.000 description 1
239000010985 leather Substances 0.000 description 1
108010062085 ligninase Proteins 0.000 description 1
235000020778 linoleic acid Nutrition 0.000 description 1
OYHQOLUKZRVURQ-HZJYTTRNSA-N linoleic acid group Chemical group C(CCCCCCC\C=C/C\C=C/CCCCC)(=O)O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 1
RLAWWYSOJDYHDC-BZSNNMDCSA-N lisinopril Chemical compound C([C@H](N[C@@H](CCCCN)C(=O)N1[C@@H](CCC1)C(O)=O)C(O)=O)CC1=CC=CC=C1 RLAWWYSOJDYHDC-BZSNNMDCSA-N 0.000 description 1
229960002394 lisinopril Drugs 0.000 description 1
210000001161 mammalian embryo Anatomy 0.000 description 1
238000007726 management method Methods 0.000 description 1
239000002609 medium Substances 0.000 description 1
210000004779 membrane envelope Anatomy 0.000 description 1
210000000473 mesophyll cell Anatomy 0.000 description 1
150000002739 metals Chemical class 0.000 description 1
229930182817 methionine Natural products 0.000 description 1
244000005700 microbiome Species 0.000 description 1
230000011278 mitosis Effects 0.000 description 1
ZAHQPTJLOCWVPG-UHFFFAOYSA-N mitoxantrone dihydrochloride Chemical compound Cl.Cl.O=C1C2=C(O)C=CC(O)=C2C(=O)C2=C1C(NCCNCCO)=CC=C2NCCNCCO ZAHQPTJLOCWVPG-UHFFFAOYSA-N 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
230000035772 mutation Effects 0.000 description 1
235000019645 odor Nutrition 0.000 description 1
150000002482 oligosaccharides Polymers 0.000 description 1
239000004006 olive oil Substances 0.000 description 1
230000003647 oxidation Effects 0.000 description 1
238000007254 oxidation reaction Methods 0.000 description 1
239000002540 palm oil Substances 0.000 description 1
239000006072 paste Substances 0.000 description 1
108010087558 pectate lyase Proteins 0.000 description 1
108010072638 pectinacetylesterase Proteins 0.000 description 1
102000004251 pectinacetylesterase Human genes 0.000 description 1
108020004410 pectinesterase Proteins 0.000 description 1
238000010647 peptide synthesis reaction Methods 0.000 description 1
239000000816 peptidomimetic Substances 0.000 description 1
150000008105 phosphatidylcholines Chemical class 0.000 description 1
229940067605 phosphatidylethanolamines Drugs 0.000 description 1
150000008106 phosphatidylserines Chemical class 0.000 description 1
108010002267 phospholipase D2 Proteins 0.000 description 1
235000011007 phosphoric acid Nutrition 0.000 description 1
229910052698 phosphorus Inorganic materials 0.000 description 1
239000011574 phosphorus Substances 0.000 description 1
102000020233 phosphotransferase Human genes 0.000 description 1
230000000704 physical effect Effects 0.000 description 1
229920002704 polyhistidine Polymers 0.000 description 1
238000006116 polymerization reaction Methods 0.000 description 1
229920001282 polysaccharide Polymers 0.000 description 1
239000005017 polysaccharide Substances 0.000 description 1
150000004804 polysaccharides Chemical class 0.000 description 1
210000001236 prokaryotic cell Anatomy 0.000 description 1
125000006239 protecting group Chemical group 0.000 description 1
229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
JSDRRTOADPPCHY-HSQYWUDLSA-N quinapril Chemical compound C([C@@H](C(=O)OCC)N[C@@H](C)C(=O)N1[C@@H](CC2=CC=CC=C2C1)C(O)=O)CC1=CC=CC=C1 JSDRRTOADPPCHY-HSQYWUDLSA-N 0.000 description 1
229960001455 quinapril Drugs 0.000 description 1
239000002994 raw material Substances 0.000 description 1
238000003259 recombinant expression Methods 0.000 description 1
238000011084 recovery Methods 0.000 description 1
230000008521 reorganization Effects 0.000 description 1
230000010076 replication Effects 0.000 description 1
230000003362 replicative effect Effects 0.000 description 1
238000011160 research Methods 0.000 description 1
108010035322 rhamnogalacturonan acetylesterase Proteins 0.000 description 1
230000005070 ripening Effects 0.000 description 1
230000028327 secretion Effects 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
238000012163 sequencing technique Methods 0.000 description 1
210000004974 shell Anatomy 0.000 description 1
230000035939 shock Effects 0.000 description 1
229910052709 silver Inorganic materials 0.000 description 1
239000004332 silver Substances 0.000 description 1
210000003491 skin Anatomy 0.000 description 1
239000004055 small Interfering RNA Substances 0.000 description 1
235000012424 soybean oil Nutrition 0.000 description 1
238000012289 standard assay Methods 0.000 description 1
239000008107 starch Substances 0.000 description 1
235000019698 starch Nutrition 0.000 description 1
229940114926 stearate Drugs 0.000 description 1
239000000725 suspension Substances 0.000 description 1
239000003765 sweetening agent Substances 0.000 description 1
238000001308 synthesis method Methods 0.000 description 1
239000006188 syrup Substances 0.000 description 1
235000020357 syrup Nutrition 0.000 description 1
230000009885 systemic effect Effects 0.000 description 1
239000003760 tallow Substances 0.000 description 1
235000019640 taste Nutrition 0.000 description 1
108060008226 thioredoxin Proteins 0.000 description 1
229940094937 thioredoxin Drugs 0.000 description 1
230000009466 transformation Effects 0.000 description 1
102000003601 transglutaminase Human genes 0.000 description 1
239000012588 trypsin Substances 0.000 description 1
238000011144 upstream manufacturing Methods 0.000 description 1
239000004474 valine Substances 0.000 description 1
230000002792 vascular Effects 0.000 description 1
230000000007 visual effect Effects 0.000 description 1
238000001238 wet grinding Methods 0.000 description 1
108010083879 xyloglucan endo(1-4)-beta-D-glucanase Proteins 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)

Landscapes

Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
Microbiology (AREA)
Biotechnology (AREA)
Biomedical Technology (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Medicinal Chemistry (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Enzymes And Modification Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Cosmetics (AREA)

CA002611071A 2005-05-31 2006-05-23 Hydrolases, acides nucleiques codant pour celles-ci et procedes de fabrication et d'utilisation de celles-ci Abandoned CA2611071A1 (fr)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US68602005P	2005-05-31	2005-05-31
US60/686,020		2005-05-31
PCT/US2006/020169 WO2007055735A2 (fr)	2005-05-31	2006-05-23	Hydrolases, acides nucleiques codant pour celles-ci et procedes de fabrication et d'utilisation de celles-ci

Publications (1)

Publication Number	Publication Date
CA2611071A1 true CA2611071A1 (fr)	2007-05-18

Family

ID=38023721

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA002611071A Abandoned CA2611071A1 (fr)	2005-05-31	2006-05-23	Hydrolases, acides nucleiques codant pour celles-ci et procedes de fabrication et d'utilisation de celles-ci

Country Status (3)

Country	Link
EP (1)	EP1910530A2 (fr)
CA (1)	CA2611071A1 (fr)
WO (1)	WO2007055735A2 (fr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
ES2665879T3 (es)	2007-09-12	2018-04-30	Dsm Ip Assets B.V.	Método para producir aceite biológico usando un fermentador no estéril
CN102844418B (zh)	2010-03-12	2015-11-25	杜邦营养生物科学有限公司	方法
WO2011158203A1 (fr)	2010-06-17	2011-12-22	Danisco A/S	Procédé
CA2825632C (fr)	2011-02-23	2020-07-07	Dupont Nutrition Biosciences Aps	Procede de traitement d'huile vegetale
AR085251A1 (es)	2011-02-23	2013-09-18	Danisco	Proceso para tratar aceite vegetal
US8268305B1 (en)	2011-09-23	2012-09-18	Bio-Cat, Inc.	Method and compositions to reduce serum levels of triacylglycerides in human beings using a fungal lipase
WO2013104660A1 (fr)	2012-01-13	2013-07-18	Dupont Nutrition Biosciences Aps	Procédé de traitement d'une huile végétale comprenant l'hydrolyse de chlorophylle ou d'un dérivé de chlorophylle et une neutralisation caustique partielle
WO2013160372A1 (fr)	2012-04-27	2013-10-31	Dupont Nutrition Biosciences Aps	Procédé de traitement d'huile végétale comprenant l'ajout de doses successives de chlorophylle ou d'une enzyme de dégradation de dérivé de chlorophylle
US9796989B2 (en)	2014-07-03	2017-10-24	Sustainable Bioproducts Holdings, Llc	Acidophilic fusarium oxysporum strains, methods of their production and methods of their use
CN104715320B (zh) *	2015-01-22	2017-02-22	青岛大学	一种综合评价纺纱原料品质的方法
SG10201911178QA (en)	2016-03-01	2020-01-30	Sustainable Bioproducts Inc	Filamentous fungal biomats, methods of their production and methods of their use
CN119014534A (zh)	2017-08-30	2024-11-26	芬德集团公司	具有丝状真菌的食用组合物和用于其培养的生物反应器系统
EA202192352A1 (ru)	2019-02-27	2022-01-26	Дзе Финдер Груп, Инк.	Пищевые материалы, содержащие частицы нитевидных грибов, и дизайн мембранного биореактора
CN118599149A (zh)	2019-06-18	2024-09-06	芬德集团公司	真菌织物材料和皮革类似物
CN116024097B (zh) *	2022-08-30	2023-11-21	四川农业大学	一株戴尔根霉菌sicau-z1及其应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EA010903B1 (ru) *	2002-04-19	2008-12-30	Дайверса Корпорейшн	Фосфолипазы, нуклеиновые кислоты, кодирующие их, и способы их получения и применения
MX295545B (es) *	2003-03-07	2012-02-03	Diversa Corp	Hidrolasas, acidos nucleicos que las codifican, y metodos para hacerlas y usarlas.

2006
- 2006-05-23 CA CA002611071A patent/CA2611071A1/fr not_active Abandoned
- 2006-05-23 WO PCT/US2006/020169 patent/WO2007055735A2/fr not_active Ceased
- 2006-05-23 EP EP06844108A patent/EP1910530A2/fr not_active Withdrawn

Also Published As

Publication number	Publication date
WO2007055735A3 (fr)	2008-10-02
EP1910530A2 (fr)	2008-04-16
WO2007055735A2 (fr)	2007-05-18

Publication	Publication Date	Title
AU2004277368B2 (en)	2011-01-20	Hydrolases, nucleic acids encoding them and mehods for making and using them
US9499844B2 (en)	2016-11-22	Compositions and methods for making and modifying oils
US20090297495A1 (en)	2009-12-03	Hydrolases, nucleic acids encoding them and methods for improving paper strength
EP2216403A2 (fr)	2010-08-11	Estérases et acides nucléiques associés et procédés
EP2329032B1 (fr)	2014-04-30	Procédés utilisant des polypeptides ayant une activité hydrolase
US9238804B2 (en)	2016-01-19	Hydrolases, nucleic acids encoding them and methods for making and using them
CA2611071A1 (fr)	2007-05-18	Hydrolases, acides nucleiques codant pour celles-ci et procedes de fabrication et d'utilisation de celles-ci
US20100129882A1 (en)	2010-05-27	Hydrolases, nucleic acids encoding them and methods for making and using them

Legal Events

Date	Code	Title	Description
2010-05-25	FZDE	Discontinued