CA2646779A1 - Bioscience ix - Google Patents

Abstract

Description

Description The primary objectives of this invention is to mass produce (gametes) eggs (especially)/sperm for initiation of stem cells sources and artificial fertilization and in vitro surrogate implantation.
This technology can be used an any and all life forms (sometimes using cross sources of hormones and or hormone analogs)...

The reason we want to produce these substances externally, outside of the body is because it is invasive to remove (eg. oocytes/eggs) from the female's ovaries versus mass production of multiple external ovaries.

We need ovarian and testicular tissue. And we need to mature the tissue.

1. We could remove a sample of ovarian and testicular tissue and regenerate to its full normal size by exposing it to HOX and ECM (Extra Cellular Matrix) .

2. We could take an ovaries and testes from recently deceased donors and wash with detergent and use the Extra Cellular Matrix Skeleton/Scaffold and repopulate with stem cells and converted stem cells into differentiated cells that normally belong in the specific places. ECM and Hyaluronic Acid and Hyaluronan and hyaluronate (Hasl, Has2, Has3) and its fibronectin-type (collagen and also laminin) components together with HOX can be used to regenerate and grow any and all life forms.
Hyaluronic also supports virulence and could be used for further augmenting the sexual maturity and successful fertilization of healthy in vitro any and all progeny...

3. Alternate method of proliferating any and all life. External cues originating outside the cell, (eg epidermal growth factor (EGF)), induce proliferative signaling via beta-catenin, an inside the cell protein is necessary in cell adhesion at the plasma membrane and transcription of cell cycle genes in the nucleus (especially epithelial cells). The factor Writ 3a, is an agonist of beta-catenin transcriptional activity, that induces greater signaling than either of the ligands (EGF and Writ 3a) alone.
However each uses a different sub-cellular pool of beta-catenin. WNT signaling with either Wnt3a, LiCI (causes the accumulation of beta-catenin and also is a dopamine antagonist, see Re: Induced Spawning - section below - GnRH, Gonadotrophin, and other hormones...), or the constitutively active S33Y
mutant of 13-catenin. WNT factors and (3-catenin also affect lymphocyte progenitor fate as well as stem cell self-renewal Stimulation with LiCl markedly increased the amounts of total and nonphosphorylated R-catenin in the myeloma cell lines tested.

4. Another option is with antigenic peptide p33 and a weaker agonist A4Y
ligand and mixed with LiCI or KCI, and pulsed with [ H]thymidine to proliferate all life (including human stem cells). Also IL-2 production had a large increase because of the lithium, as measured by proliferation of the IL-2-dependent, it has been postulated that GSK-3 regulates the T lymphocyte responses. GSK-3 phosphorylates NF-Atc (nuclear factor of activated T cells (NFAT)), which promotes cytoplasmic localization and also export from the nucleus. Therefore, the presence of LiCI should inactivate GSK-3, resulting in prolonged dephosphorylation of NF-ATc and sustained nuclear localization. GSK-3 plays an important role in antigen-specific T cell activation by regulating NF-ATc localization.

5. Once the stem cells or any other tissue proliferated using this method is ready to be grafted back into a patient's body (eg. after regenerating the body part), we can use a beta-catenin antagonist protein E-cadherin to treat the cells to avert cancer...

6. Both 1. and 2. and 3. and 4. are grown outside the body - external ovaries/testicular organs (the result is a regenerated external (once fully regenerated, mature ovary/testes), they can be used as a sort of egg and sperm mass production factories... these can be grown in Gerard Voon's patented bioreactor that uses synthetic blood to bring oxygen and remove CO2 from the tissue/body part and either dialysis and/or functional external from the body kidney that filters waste that is also via the bioreactor filters the blood and uses it for its own survival as well; at the other end nutrients and vitamins, growth hormones and supplements are added based on the stage of growth.. .of the best recipe. In addition to ovarian and testes tissue the above techniques can be used for any and all body parts (from healthy pancreas cells (reinjected - possibly differentiated from stem precusor cells) to full regenerated pancreas to naturally produce insulin for transplant back into the patient's body (Dad)), also immune cells... glands, organs and tissues. Other uses include regenerating and culturing swallows salivary glands and solving the cues, environmental and/or hormonal cues... any and all cues that trigger swallows to make bird's nest soup... also we could regenerate Mako shark's fins separate from the body, possibly from stem cells... In the case (of growing/differentiating ovarian/testes tissue from stem cells) we need to mature the ovaries/testicles... There are both environmental and internal factors (for water bourne creatures) that are involved and even necessary for maturation.. .there are environmental factors include: (eg. simulate annual seasons - including monsoon season); most important is photoperiod (melatonin might be used to alternate use during winter and non-use during the longer lighting summer) length of daylight and (also variation in intensity and/or light pulses) relative to the length of darkness), proper stimulation olfactory organs, FEO (food entrained oscillator... proper stimulation of taste buds, food availability; water temperature; water flow rate (activity - possible impacts on metabolism )(velocity m/sec - flooding and water currents); pH/DH (water hardness); Dissolved Oxygen (mg/I); Free carbon-dioxide (mg/I); Total alkalinity (mg/I); Chlorides (mg/I); TDS (mg/I); DOM (mg/I);
Specific conductivity (mhos)/25C, some believe in the cycles of the moon, atmospheric pressure, availability and desired quality types based on the fish type of spawning substrate, availability of aquatic plants, sticks, gravel, and safe nooks and crannies, nutrition, and the presence of other fish...The effects of these external factors on the blood concentrations of substances (eg. exercise and activity and changes in PH/DH... and/or melatonin antagonists) could be used together with GnRH, gonadotrophin, vitamin A (beta carotene), retinoic acid, for maturing the gametes which is absolutely necessary for spawning/mating/sexual intercourse/breeding to result in healthy embryos (mitosis/meiosis)...

7. Responses by gonadotrophin induced cycles, for females (vary) (some ovulating 70 eggs), we need alleles to induce the follicles to mature, it seems follicular fluid from large follicles (I-pFF), to mature oocyte cytoplasmic and nuclear maturation... It has been hypothesized that Alleles contributing to induction of follicle maturation and steriodogenesis, that (whereby) the maturation process is subject to gonadotropin inducing cycles, contribute to under environmental factors, to mature follicles, to produce estrogens to create and LH surge and thus ovulating.

8. We could also use ovarian tissue and/or full ovaries to produce eggs such as sturgeon (caviar) and turtle and/or crab eggs... this technology could be used to produce any and all eggs...

We can also use synthetic meiosis-activating sterol, derived from follicular fluid (FF-MAS).
Re: Inducing Maturity of Gametes from Ovarian and Testicular Tissue Vitamin A in the form of beta-carotene (BC) an also retinol ester, is supplied to oocytes and cumulus-granulosa via the ovarian follicles (by binding with molecules).
Vitamin A, retinoic acid are involved artificial insemination, and in artificial reproductive techniques (ART), eg.
superovulation, ovum pick up, and in vitro maturation culture. RA is possibly involved in affecting cytoplasmic maturation through gene expression of gonadotrophin receptors, midkine, cyclooxygenase-2, and nitric oxide synthase in cumulus-granulosa cells, oocytes, blastocyst, follicles, gametes (any and all ovarian and/or testes cells).

Tissues needed for the hormonal cascade are the hypothalamus, pituitary gland, and gonads...
The cascade of hormones are gonadotropin releasing hormones (GnRH), which travel from the hypothalamus to the pituitary gland... The pituitary regulates growth and reproduction (we could also use it to further enhance stem cell proliferation). Specialized pituitary cells are signalled by GnRH to release gonadotropic hormones. These gonadotropic hormones affect the gonads, that then produce steroids that regulates final maturation of the gametes.

Maturation of the egg includes vitellogenesis, in this process the yolk proteins (we could grow fully complete livers (deceased donor, detergent and ECM matrix with HOX
and/or stem cells and/or specialized cells per portion of belong on the matrix) outside the body and produce (in Gerard Voon's patented bioreactors)) externally produced yolk protein (mass production) are produced in the liver, transport it to the ovary, to produce much egg size.
The yolk is necessary for the embryo growth.

We are studying the induction and its contribution of Germinal vesicle migration and germinal vesicle breakdown (GVBD) to the gametes development.

Re: Next Stage After Gametes have Matured At this stage (when the gametes have matured) prostaglandins are produced.
They induce ovulation, rupturing of the follicle cells holding the egg. The egg makes its way to the body cavity or ovarian lumen... Interestingly after ovulation, the health of eggs can decrease.

Re: Induced Spawning In addition to viagra, cialis, water melon rind, Kapok (Ceiba pentandra) bark We can induce spawning by extracting hCG from the urine of pregnant (large sources that are easy to grow and breed) - any and all sources eg.
cows/pigs/horses/dogs/cats...

Major methods include injection of a GnRH analogue; agonist (Leutinizing Hormone Releasing Hormone, LHRHa) with dopamine possible antagonist:

Dopamine blockers and depletors include:
= metoclopramide = pimozide (Orap) = haloperidol (Haldol) = olanzapine (Zyprexa) = tetrabenazine = chlorpromazine hydrochloride (Thorazine) = domperidone = droperidol (Inapsine) = fluphenazine hydrochloride (Prolixin) = metoclopramide (Reglan) = perphenazine (Trilafon) = prochiorperazine (Compazine) = thiethylperazine (Torecan) = trimethobenzamide (Tigan) = reserpine (Serpalan) - the fish may be reacting to a change in serotonin level and injection of gonadotropin and hCG (Human Chorionic Gonadotropin), Ovopel, Ovaprim-C, Ovatide, Ovaplant, CPE
(Carp Pituitary Extract) - we also plan to use the above hormones to proliferate stem cells and/or specialized cell regeneration).

Genetic (chromosomal modification)...to induce polyploidy, gynogenesis and androgenesis can be used for all life (aiming to control sex, inhibit sexual maturation and rapid inbreeding -Thorgaard, 1986). Methods to include using temperature sock, high hydrostatic pressure and/or chemicals... on fertilized eggs. Usage of these methods at the final stages of meiosis will retain a second polar body resulting in triploids, furthermore later usage of such methods avoids first cleavage resulting in tetraploidy...

Irradiating the sperm results in gynogenic progeny (progeny having only mother's genes)... Irradiating pre fertilized eggs and then fertilizing results in androgenic progeny (progeny having only the father's genes)... Preventing first cell cleavage restores diploidy.
Methods used can obtain all-female population and/or all0female triploid populations. Start with gynogenic progeny... hormones are used to reverse the sex of the population to phenotypes of males but genetically female. These quasi-males fertilize normal female eggs, that produce all female progeny, the goal is get rid of inbreeding via gynogenic males are prevented.

These methods can be used to produce (via sample from live donor to regenerate - including stem cells - into full matured) ovarian and testicular tissue (and/or collect gametes directly from recently deceased donors) to use in in vitro fertilization.

Re: Artificial Water Bourne creatures' Gills By growing (via stem cells and/or regeneration/cloning of the body parts) or removing gills from the most suitable fish... we could filter out oxygen and release carbon dioxide for divers, submarines, underwater stations and/or use the carbon dioxide for any and all algae and plant growth.

Re: Melatonin Antagonist for growth and/or sexual maturity Bright light therapy is a popular method which prevents the conversion of serotonin to melatonin. The same results from more time in natural sunlight.

Since extending photoperiod is the primary means of inducing fish to mature and spawn (life cycles), and melatonin (produced by the pineal gland during darkness in the environment - the result of our visual receptors) is known to reduce rate of growth and sexual maturity therefore we are using melatonin antagonists including: muscarinic cholinergic antagonist;
mecamylamine, a nicotinic cholinergic antagonist; atenolol, a [3-adrenergic antagonist;
phentolamine, a nonselective a-adrenergic antagonist; prazosin, a selective a-adrenergic antagonist; haloperidol, a dopaminergic antagonist; naloxone, an opioid antagonist; and ibuprofen, a cyclooxygenase inhibitor. The following are theorized that hormones... are facilitated (some how involved) affected by the acetylcholine, dopamine, and/or prostaglandin.
Using (S20098: N-[2-(7-methoxy-1-naphthyl)ethyl]acetamide), and a Putative Antagonist (S20928: N-[2-(1-naphthyl)ethyl]cyclobutyl carboxamide to Block the Effects of Melatonin S20098, an agonist working on melatonin receptors. S20928 have both agonist/antagonist effects, at low applications is an antagonist on melatonin receptors in the suprachiasmatic nucleus and intergeniculate leaflet.

Further Melatonin Agonists and/or Antagonists that we are using to Pharmacologically Take the place of Photoperiod Inducing Growth and Sexual Maturity PREVIOUS NAMES Mel I a Mel1 b ML2 MLIA MLIB

STRUCTURAL INFORMATION 350 as (human) 363 as (human) FULL AGONISTS Melatonin (M 5250) Melatonin (M 5250) 2-lodomelatonin (1 1899) 2-lodomelatonin (1 1899) 2-lodomelatonin (11899) 6-Chloromelatonin (C 0331) N-Propionyl melatonin N-Propionyl melatonin Melatonin (M 5250) N-Butanoyl melatonin N-Butanoyl melatonin N-Acetylserotonin (A 1824) 6-Chloromelatonin (C 0331) 6-Chloromelatonin (C 0331) 5-MCA-NAT
2-Methyl-6,7-dichloromelatonin 2-Methyl-6,7-dichloromelatonin (-)-AMMTC (-)-AMMTC
11K7 (I 5531) PARTIAL AGONISTS 5-Methoxyluzindole 5-Methoxyluzindole Not known N-Acetyltryptamine (A 7342) N-Acetyltryptamine (A 7342) ANTAGONISTS Luzindole (L 2407) Luzindole (L 2407) Luzindole (L 2407) S20928 S20928 Prazosin (P 7791) 4P-PDOT N-Acetyltryptamine (A 7342) K185 (K 1888) SIGNAL TRANSDUCTION MECHANISMS Gi (cAMP modulation) Gi (CAMP modulation) Gq/11 (increase IP3/DAG) Gq/1 1 (increase IP3/DAG) cGMP modulation RADIOLIGANDS OF CHOICE 2-[1251]-lodomelatonin 2-[1251]-lodomelatonin 2-[1251]-lodomelatonin [3H]-Melatonin [3H]-Melatonin 2-[1251]-MCA-NAT
ABBREVIATIONS
4P-ADOT: 4-Phenyl-2-acetamidotetralin (-)-AMMTC: N-Acetyl-4-aminomethyl-6-methoxy-9-methyl-1,2,3,4-tetrahydrocarbazole 4P-CADOT: 4-Phenyl-2-chloroacetamidotetralin GR 196429: N-[2-[2,3,7,8-tetrahydro-1 H-furo(2,3-g)indol-1-yl]ethyl]acetamide I IK7: N-Butanoyl-2-(2-methoxy-6H-isoindolo[2,1-a]indole-l 1-yl)ethanamine K185: N-Butanoyl-2-(5,6,7-trihydro-11-methoxybenzo[3,4]cyclohept[2,1 -a]indol-yl)ethanamine Luzindole: 2-Benzyl-N-acetyltryptamine 5-MCA-NAT: 5-Methoxycarbonylamino-N-acetyltryptamine Melatonin: 5-Methoxy-N-acetyltryptamine 4P-PDOT: 4-Phenyl-2-propionamidotetralin 8M-PDOT: 8-Methoxy-2-propionamidotetralin S20098: N-[2-(7-Methoxy-1-naphthalenyl)ethyl]acetamide S20928: N-[2-Naphth-1-yl-ethyl]-cyclobutyl carboxamide Melatonin Receptors Re: Fish Blocking Ca2+ As Well, Vitamin D's Role in Ca2+ absorption Calcium was blocked by diluting water, removing calcium from the diet or by long term feeding of vitamin D-deficient diet. Melatonin. production is affected by plasma Ca2+.
Another way to influence the effects of melatonin is via iontophoresis of the Ca2+ ionophore A-23187, prevents the inhibitory effect of both melatonin or Ca2+ antagonists...

Transcription factors called clock genes, which include Clock, period genes (pert, per2, and per3), cryptochromes (cryl and cry2), and Bmall, regulate hypothalamic suprachiasmatic nucleus (SCN) and in peripheral tissues. The CLOCK and BMALI proteins initiate transcription of the period (per) and cryptochrome (cry) genes, and PER and CRY regulate self transcription through an interaction with the CLOCK/BMAL1 heterodimer to regulate cellular rhythms.

The SCN regylates rhythm in a several major body parts, including brain, pineal gland, adrenal liver, heart, kidney, muscle, pancreas, lung, and oviduct, through neural networks and hormonal excretion via the cerebrospinal fluid. It is clear that the same genes that are responsible for SCN rhythmicity are rhythmically expressed throughout the body.

Anyone who has night time cravings for food must wonder, there seems to be a clinging bond between the time, darkness (lack of activity) and other cues that motivates (possibly metabolism since an early dinner), you such as your rhythm has entrained you to crave food in the same daily rhythm ... This could be the cause of FEO (food entrained oscillator)... The FEO

is an alternate circadian clock acting on any and all living organisms, in addition to suprachiasmatic nuclei (SCN).

The necessary nutrients, include metabolites, ions, vitamins, nucleotides, and amino acids can also traverse from cumulus cells to the oocyte via GJC. Variations in these nutrients inputs can impact positively and/or negatively the developments from stage to stage needs of an ovary tissue into an embryo...

Measurement of carbohydrate concentrations can be taken via Glucose, pyruvate and lactate concentrations in FF, plasma and maturation medium.

Amino acid profiles (concentrations) can be Measured In follicular fluid (FF), and/or plasma... for each oestrous stage.

Measurement of osmolality can be measured using pooled FF, independent of oestrous stage.
Amino acids EAA
L-Arginine HCI
L-Cystine L-Glutamine L-Histidine HCIIH
L-Isoleucine L-Leucine L-Lysine-HCI
L-Methionine L-Phenylalanine L-Threonine L-Tryptophan L-Tyrosine L-Valine L-Glutamine NEAR
L-Alanine L-AsparagineHO
L-Aspartic acid L-Glutamic acid Glycine L-Proline L-Serine Minimal Essential Medium (MEM) essential amino acid solution (no.
320-1130; Gibco).
b MEM nonessential amino acid solution (no. 320-1140; Gibco).

Specifically (stage by stage) Amino acids affected by oestrous stage included:
glutamate, asparagine, histidine, glutamine, threonine, arginine, taurine, alanine, tyrosine, tryptophan, methionine, valine, phenylalanine, leucine and lysine. The Stage I FF amino acid profile was very similar to that of plasma, particularly as regards aspartate, glutamate, serine, glycine, threonine, arginine, taurine, phenylalanine and isoleucine. By contrast, Stages Il-IV profiles were significantly lower than plasma for aspartate, glutamate, asparagine (Stage Ill only), serine, taurine (except Stage II), alanine (Stage II only), methionine (except Stage II), valine (except Stage II), leucine (Stage III only) and lysine (except Stage III).
Overall, plasma levels were markedly higher than those of FF for glutamate, serine and glutamine and, to a lesser extent, for asparagine, threonine, taurine, tyrosine, methionine, phenylalanine and lysine. By contrast, FF valine concentration was significantly higher than in plasma.

It is well believed that amino acids are uptaken into the oocytes by the gap junctions (gap junctional communication (GJC)).
Addtionally the GSH (glutathione) content of an oocyte is much dependent nextdoor cumulus cells, GJC is believed to contribute to GSH content in the oocyte Via passage/uptake of GSH or its substrates, cysteine and glutamine from the cumulus cells to an oocyte. The way cumulus cells regulate MPN (male pronuclei) formation is not understood.
The basic medium used for the maturation of oocytes was BSA-free Whitten's medium NaCl, KC1, KH2PO 4, MgSO 4, NaHCO 3, glucose, sodium lactate, sodium pyruvate, hemicalcium lactate, potassium penicillin, streptomycin sulfate, phenol red and supplemented with PFF (pig/cow/human) follicular fluid, TCM-199 containing hCG, eCG, glutamine, fetal calf serum...

Also, large accumulation of cumulus cells (eg. Artificial Insemination with an external ovary produced oocyte - more broadly the cumulus are responsible for Routing and Penetration of spermatoza) affects the success of penetration of spermatozoa And the large cumulus cell mass induces acrosome reaction and assists penetration into the oocyte.
(Jason 0.5%) (DLD 0.25%) (GP HW 0.25%) We can use Proton nuclear magnetic resonance ((1)H NMR) to chemically dissect any and all life fluids, including stem cell developments (proliferation stages), regeneration, meiosis, mitosis, different cells and the functions, liver and kidney filteration cells the mechanism whereby waste enters one end and is separated and the clean blood diverted back into the system, the mechanism of lung cells (including fish gills) how the absorb oxygen and release carbon dioxide and the membrane? And how this membrane performs this function... (each and every cell type and their symbiotic relationship with each and every organ and glands...
development of ovaries to oocyte, the changing make up of Newt/Salamander's (any and all amphibians (GP) regenerating tails, starfishes, jellyfish, sea cucumber, ECM/HOX changes in response to amino acids, vitamins, ions... changes, mammals/ fishesturtles (near extinct species), Focusing on follicular growth and maturation, (1)H NMR is utilized to dissect intrafollicular and circulating glycoconjugates (sugar chains and N-acetyl groups), lipoproteins (CH(3) and CH(2) groups), glucose metabolites (trimethylamines, acetate and lactate), amino acids (including the above and more specifically: (glutamine/glutamate and alanine), creatine/creatinine and polyamines). Follicular fluids is measured for oestradiol and progesterone. The intrafollicular alanine and lipoproteins (CH(3) groups) went down in the dominant follicle (the future oocyte) during growth stage, while progesterone and oestradiol showed a large increase. As detailed above gonadotrophin is used to induce ovulation;
follicular maturation is noted by lessening of glycoconjugates (sugar chains), trimethylamines and acetate, less oestradiol, also additional CH(3) groups of lipoproteins and progesterone.
Re: The Idea of Varying levels of Factors for Different Phases in Mitosis for any and all Proliferation Promoting Substances has been Previously Patented by Gerard Voon, but Below is one Example of one such Substance (Ca2+) Ca2+ ions needed for mitogenesis and increase in cytosolic Ca 2+ acts as a physiological trigger in cell proliferation, the different phases of the cell cycle present different dosage needs for Ca2+. Ryanodine and NiCl2 eliminated cytosolic Ca + and did not prevent PDGF-induced development into the Go into G, phase. Furthermore progression of cells into the S phase and the further mitosis were arrested, perhaps because S phase needs Ca 2+ and further proliferation promoting factors is regulated by Ca2+dosages and perhaps sharp changes and maintenance levels. G,/S are arrested when there is not enough Ca2+. Emptying of Ca2+ pools during Go/G, and S arrest growth while plating positive affect on G2 and M
cell cycle phases.
PDGF-induced (any and all) cells' proliferation requires the activity of SERCA
pumps to uptake Ca2+ pools the entry into the S phase.

Ca2+ timing (in correlation with the mitotic phases) seems to involve Ca2+
intracellular pools drainage and alternating usage of Ca2+ membrane to uptake the exogenous Ca2+
dependent (are necessary for) on the mitotic phase. PDGF (Platelet-derived growth factor) and possibly any and all other factors (especially for proliferation) binding to receptors at the surface of cellular membrane and are intertwined with changes in cytosolic Ca + dosages that involves a surge the a lesser steady-state elevation, involving both the drainage of Ca +
pools and uptake of Ca 2+ from the extracellular (external to the cell).

Re: Reactive Oxygen Species (ROS), reactive nitrogen species, and calcium (Ca2+) It has been suggested that plant cells sense ROS via at least three different mechanisms: (i) unidentified receptor proteins; (ii) redox-sensitive transcription factors, such as NPRI or Heat Shock Factors; and (iii) direct inhibition of phosphatases by ROS. There is a possiblilty of interactions of ROS, reactive nitrogen species, and calcium signalling.

All three are involved in control and regulation of biological processes, such as growth, cell cycle, programmed cell death, hormone signaling, biotic and abiotic stress responses and development... and any and all phenotypic responses.

ROS, reactive nitrogen species, and calcium (Ca2+) signalling are generated at a number of intracellular locations, including mitochondria, chioroplasts, peroxisomes, and at the extracellular side of the plasma membrane. These locations signal transduction events, such as mitogen-activated protein kinase cascades, eliciting specific cellular responses. The impacts of these ROS, reactive nitrogen species, and calcium signalling on cellular processes is mediated by both the perpetuation of their production and their amelioration by scavenging enzymes such as superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT). We need further study location, amplitude, and duration of ROS, reactive nitrogen species, and calcium Functioning like Ca2+ signaling ROS (And possibly nitrogen species) is controlled by the spatial and temporal nature of its pools and drainage.

Re: Some of the Fish we Plan to Apply the Above Spawning Methods and Techniques (From External Ovaries Factories to ROS... ) Scientific name Common name Acanthopagrus latus Yellowtn sea bream A. berda Picnic sea bream A. schiegeli Black sea bream A. sivicolus Southern black sea bream Anthias disper Red fish Boleophthalmus pectinirostris Pond or mud skipper Chanos chanos Milkfish Choerodon schoenleinii Black spot tusk fish Cromileptes altivelis Highfin grouper Eleutheronema tetradactylum Four finger threadfin Epinephelus akaara Red grouper E. amblycephalus* White-spotted green grouper E. awoora Yellow grouper E. coioides / suillus Red-spotted grouper E. fario Black-saddled grouper E. fuscoguttatus Tiger grouper E. lanceolatus Giant or King grouper E: malabaricus I salmonides Malabar grouper E. quoyaqnus Long-finned grouper E. tauvina Green grouper E. tukula Potato grouper E. trimaculatus Brown marbled grouper Evynnis cardinalis Golden-skinned porgy Girella melanichthys Smaliscale blackfish Girella puncta* Largescale blackfish Glossogobius giuris Flathead goby Gnathanodon speciosus Kingfish Hapalogenys nitens Beard grunt Kyphosus lembus Shortfin rudderfish Lateolabrax japonicus Japanese sea bass Lates calcarifer Asian sea bass Lethrinus nebulosus Green snapper Liza macrolepis Largescale liza Lutjanus argentimaculatus Mangrove red snapper L. erythropterus Pink snapper L. johnii John's snapper L. malabaricus Firespot snapper L. monostigma Onespot snapper L. russelli Russell's snapper L. sebae Emperor snapper L. stellatus Spotted snapper Miichthys miiuy Nibe or brown croaker Mugil cephalus Grey mullet Nibea diacanthus Speckled drum Oplegnathus punctatus Spotted knifejaw Pagrus major Red sea bream Platax orbicularis Narrow-banded batfish Plectorhynchus cinctus Three-banded grunt P. pictus Three-lipped grunt Plectropomus leopardus Coral trout Polynemus plebejus Common threadfin P. sexfilis Six threadfin Pomadasys kaakan Lined silver grunter Psettodes erumei Big-mouthed flounder Pseudosciaena crocea Large yellow croaker Rachycentron canadum Cobia or Sergeantfish Scatophagus argus Spotted scat Sciaenops ocellatus Red drum Seriola dumerilli Greater yellowtail Siganus fuscescens Dusky spinefoot S. guttaus Speckled spinefoot S. oramin Yellow-spotted spinefoot Sillago sihama Sand borer S. vermiculatus Reticulated rabbiffish Sparus sarba Silver sea bream Takifu rubripes Tiger puffer Terapon jarbua Three stripe tigerfish Trachinotus blochii Pompano Re: The Drugs Successfully Used for Aquaculture (below), we plan to test their efficacy for Any And All Life Table 1. Drugs Approved for Use in Aquatic Species*
Drug Species Indication Formalin Finfish Control of certain external protozoa and monogenetic trematodes Finfish eggs Control of fungi of the family Saprolegniaceae Penaeid shrimp Control of certain external parasites Oxytetracycline Pacific salmon Marking of skeletal tissue via medicated feed Finfish Marking of skeletal tissue via immersion Salmonids Control of ulcer disease caused by Hemophilus pisicium, furunculosis caused by Aeromonas salmonicida, bacterial hemorrhagic septicemia caused by A liquefaciens and pseudomonas disease.
Catfish Control of bacterial hemorrhagic septicemia caused by A liquefaciens and pseudomonas disease Lobster Control of gaffkemia caused by Aerococcus viridans Sulfadimethoxine/ Salmonids Control of furunculosis caused by Aeromonas ormetoprim salmonicida Catfish Control of enteric septicemia caused by Edwardsiella ictaluri Sulfamerazinet Rainbow, brook, Control of furunculosis and brown trout Tricaine Fish, amphibians, Temporary immobilization as an aid in handling Methanesulfonate and other poikilotherms Chorionic Finfish Spawning aid Gonadotropin *Data from FDA Center for Veterinary Medicine Web site.7 tNot currently marketed.

Re: Sites and Some Hormones that Achieve our Desire to Mature Sperm and Eggs and Induce Spawning Induction of spawning by the injection of 8,11,14-eicosatrienoic acid into the coelomic cavity, shows that the natural sperm maturation factor and spawning hormone.
Alternately females, injected with female prostomial homogenate into the coelomic cavity induces oocyte maturation and subsequent spawning. Oocytes, on the other hand, although maturing in the presence of CMF (Coelomic Maturation Factor), do not mature in the presence of prostomial homogenate.
Oocyte maturation and subsequent spawning is under the control of 2 hormones:
(1) a substance from the prostomium, Prostomal Maturation Hormone (PMH), which induces the production of (2) CMF which acts on the oocyte. PMH is required for oocyte maturation...

Re: Other Chemicals to be Used on any and all Life for Sexual Maturation/Proliferation and/or Breeding/Spawning (On Both Males and Females) Tetracaine, procaine, propranolol, oxprenolol, lanthanum chloride, ionophore A23187, DL-dithiothreitol, arachidonic acid and ammonium ions (ammonium sulphate) were also all tested for their ability to induce maturation... Also possibly injection with 5-hydroxytryptamine (5-HT) into the gonads... serotonin (and any and all psychiatric drugs including viagra and/or cialis) can be applied to induce spawning/breeding... (We could possibly use Antho-RFamide, a neuropeptide situated in ciliated neurons within follicle epithelia, also causes exfoliation of the follicle epithelium from spawned follicles). Photoperiod/intensity augments the potency of Antho-RFamide. The actin-binding toxin phalloidin substantially reduced the incidence of Antho-Rfamide.

Re: Other Alternate Serotonin Drugs that may help Induce Spawning Serotonin levels increase with of tryptophan. Increasing intake foods high in tryptophan, do affect sretonin levels due to competition with other amino acids. Stamina and aerobic exercise improves mood, by increasing in serotonin (endorphins?) levels. Endorphins (endogenous opioid polypeptide compounds). Produced by the pituitary gland and the hypothalamus in vertebrates during strenuous exercise, excitement,, and orgasm; and they resemble the opiates in their abilities to produce analgesia and a sense of well-being. Endorphins work as "natural fever relievers", whose effects may be enhanced by other medications. We could increase water flow rate and velocity and or exercise/activity that is enjoyable to the life form then extract the pituitary and/or hypothalamus from the exercising life form (eg.
pigs/cows/gold fish creatures that are easy, fast and large volume...) also the sources of endorphins, GnRH, Pituitary and Hypothalumus... extracts from the glands of pigs/cows/gold fish could be prepared for extraction via Gerard Voon's patented Neural Links (12% GP) to put the subject of extract in a serotonin/endorphin inducing mood... and inject to make other life (aquaculture and/or any and all life stock farming - even as a viagra and/or feel good supplement) mate... In fact we could use Neural Links directly on the life forms to induce them spawn/breed/mate/have sex (11 %)...

It is believed that a diet rich in whole grain carbohydrates and low in protein will increase serotonin by secreting insulin, which helps in amino acid competition. The danger is that increasing insulin for a long period of time can lead to insulin resistance, which are factors for some causes of obesity, type 2 diabetes, and even lower serotonin levels.
Furthermore that muscles use many amino acids but not tryptophan, allowing men to have more serotonin absorption than women.

Re: Drugs that treat the Serotonin/5-HT system include - AntiDepressants = Ambilify (Aripiprazole) = Sertraline (Zoloft) = Escitalopram (Lexapro) = Fluoxetine (Prozac) = Bupropion (Welibutrin, Zyban) = Paroxetine (Paxil) = Venlafaxine (Effexor) = Trazodone (Desyrel) = Amitriptyline (Elavil) = Citalopram (Celexa) = Duloxetine (Cymbalta) = Mirtazapine (Remeron) = Nortriptyline (Pamelor) = Imipramine (Tofranil) Also (concentrated extracts of) hypericum perforatum (St John's Wort) First Generation Antipsychotics Butyrophenones = Haloperidol (Haldol) Phenothiazines = Chlorpromazine (Thorazine) = Fluphenazine (Prolixin) - Available in decanoate (long-acting) form = Perphenazine (Trilafon) = Prochlorperazine (Compazine) = Thioridazine (Mellaril) = Trifluoperazine (Stelazine) = Mesoridazine = Promazine = Trrflupromazine (Vesprin) = Levomepromazine (Nozinan) = Promethazine (Phenergan) Thioxanthenes = Chlorprothixene = Flupenthixol (Depixol and Fluanxol) = Thiothixene (Navane) = Zuclopenthixol (Clopixol & Acuphase) Second generation antipsychotics = Clozapine (Clozaril) - treats schizophrenia, acute manic episodes, and maintenance of bipolar disorder.
= Risperidone (Risperdal) - treats Tourette Syndrome or Anxiety Disorder.
= Quetiapine (Seroquel) - Used primarily to treat bipolar disorder and schizophrenia, and "off-label" to treat chronic insomnia and restless legs syndrome; it is a powerful sedative = Ziprasidone (Geodon) - Now (2006) approved to treat bipolar disorder. Dosing 20 mg twice daily initially up to 80 mg twice daily. Prolonged QT interval a concern; watch closely with patients that have heart disease; when used with other drugs that prolong QT interval potentially life-threatening.
= Amisulpride (Solian) - Selective dopamine antagonist. Higher doses (greater than 400 mg) act upon post-synaptic dopamine receptors resulting in a reduction in the positive symptoms of schizophrenia, such as psychosis. Lower doses, however, act upon dopamine autoreceptors, resulting in increased dopamine transmission, improving the negative symptoms of schizophrenia. Lower doses of amisulpride have also been shown to have anti-depressant and anxiolytic effects in non-schizophrenic patients, leading to its use in dysthymia and social anxiety disorder.
= Asenapine is a 5-HT2A- and D2-receptor antagonist under development for the treatment of schizophrenia and acute mania associated with bipolar disorder.
= Paliperidone (Invega) - Derivative of risperidone.
Third generation antipsychotics = Aripiprazole (Abilify) - reduce susceptibility to metabolic symptoms seen in some other atypical antipsychotics.[41 = Dopamine partial agonists:
= Under clinical development - Bifeprunox; norclozapine (ACP-104).
Other options = Tetrabenazine (Nitoman in Canada and Xenazine in New Zealand and some parts of Europe) is similar in function to antipsychotic drugs, though is not, in general, considered an antipsychotic itself. This is likely due to its main usefulness being the treatment of hyperkinetic movement disorders such as Huntington's Disease and Tourette syndrome, rather than for conditions such as schizophrenia. Also, rather than having the potential to cause tardive dyskinesia, which most antipsychotics have, tetrabenazine can actually be an effective treatment for the condition.
= Cannabidiol One of the main psychoactive components of cannabis. A recent study has shown cannabidiol to be as effective as atypical antipsychotics in treating schizophrenia.

Metabotropic glutamate receptor 2 agonism has been seen as a promissing strategy in the development of novel antipsychotics. The active metabolite of this prodrug targets the brain glutamate receptors mGIuR2/3 rather than dopamine receptors.

Re: Natural Humane Sources of Serotonin Serotonin are in mushrooms and plants, including fruits and vegetables.
Particularily high contents are present in nuts of the walnut (Juglans) and hickory (Carya) genuses. Serotonin are been present in plantain, pineapple, banana, kiwifruit, plums, and tomatoes. Moderate contents are present in a wide range of tested vegetables. It should be noted that serotonin, unlike its precursors 5-HTP and tryptophan, does not cross the blood-brain barrier. There are plants containing serotonin together with a family of related tryptamines that are methylated at the amino (NH2) and hydroxy (OH) groups, are N-oxides, or miss the OH group eg.
Anadenanthera genus that are used in the hallucinogen.

Re: Treatment For Anxiety that may help Spawning/Breeding/Mating/Sex and also Time of Stress for eg. Aquaculture including Transport and/or Disease and/or Majoring Cleaning of the Tank, Any time the Creatures Become Spooked:
Time to Elimination Common Peak Half-Life (h)** Approximate Drug Name IR Brand (Onset of [active Primary Effects 1 Equivalent Names* H action in Dose*** -1 hours) pi metabolite]
Xanax, Alprazolam Xanor, Tafil, 1-2 6-12 hours anxiolytic 0.5 mg Alprox, Frontal(Brazil) Lexotanil, Lexotan, Bromazepam Lexomil, 1-3 10-20 hours anxiolytic 5-6 mg Somalium, Bromam Librium, 5-30 hours Chlordiazepoxide Tropium, 1.5-4 [36-200 anxiolytic 40 mg Risolid, hours]
Klopoxid Cinolazepam Gerodorm .5-2 9 h hypnotic 40 mg Klonopin, Clonazepam Klonapin, 1-4 18-50 hours anxiolytic, 0.5 mg Rivotril, anticonvulsant lktorivil Cloxazolam Olcadil 2-5 (?) 18-50 hours anxiolytic, I mg (Brazil) anticonvulsant Clorazepate Tranxene Variable [36-100 anxiolytic, hours] anticonvulsant 15 mg Valium, Apzepam, Stesolid, 20-100 hours anxiolytic, Diazepam Vival, 1-2 [36-200] anticonvulsant, muscle 10 mg Apozepam, relaxant Hexalid, Valaxona Estazolam ProSom .5-5 10-24 h hypnotic 1-2 mg Rohypnol, 18-26 hours Flunitrazepam Fluscand, .5-3 [36-200 hypnotic 1 mg Flunipam, hours]
Ronal.

Rohydorm (Brazil) Dalmadorm, [40-250 Flurazepam Dalmane 1-1.5 hours] hypnotic 20-25 mg Flutoprazepam Restas .5-9 60-90 hours hypnotic 2-3 mg Halazepam Paxipam 1-3 [30-100 anxiolytic 20-40 mg hours]

Ketazolam Anxon 2.5-3 30-100 hours anxiolytic 15-30 mg [36-200]
Loprazolam Dormonoct .5-4 6-12 hours hypnotic 1-2 mg Ativan, Lorazepam Temesta, 2-4 10-20 hours anxiolytic,anticonvulsant 1 mg Lorabenz Loramet, Lormetazepam Noctamid, .5-2 10-12 hours hypnotic 1-2 mg Pronoctan Medazepam Nobrium ? 36-200 hours anxiolytic 10 mg Dormicum, Versed, 3 hours (1.8-6 Midazolam Hypnovel, .5-1 hours) hypnotic 5-15 mg Dormonid (Brazil) Nimetazepam Erimin .5-3 14-30 hours hypnotic 5 mg Mogadon, Nitrazepam Alodorm, .5-7 15-38 hours hypnotic 10 mg Pacisyn, Dumolid Nordazepam Madar, Stilny ? 50-120 hours anxiolytic 10 mg Seresta, Serax, Serenid, Serepax, Oxazepam Sobril, 3-4 4-15 hours anxiolytic 30 mg Oxascand, Alopam, Oxabenz, Oxapax Phenazepam ? 1.5-4 60 hours anxiolytic, 1 mg anticonvulsant Pinazepam Domar ? 40-100 hours anxiolytic 20 mg Prazepam Lysanxia, 2-6 36-200 hours anxiolytic 10-20 mg Centrax Premazepam 1 2-6 10-13 hours anxiolytic 3.75 mg Quazepam Doral 1-5 39-120 hours hypnotic 20 mg Restoril, Temazepam Normison, .5-3 8-22 hours hypnotic 20 mg Euhypnos Tetrazepam Mylostan 1-3 3-26 hours Skeletal muscle 50mg relaxant Triazolam Halcion, .5-2 2 hours hypnotic 0.25 mg Rilamir [edit] Atypical benzodiazepine receptor ligands Common Brand Elimination Half- Approximate Drug Name Life (h)** [active Primary Effects Equivalent Names*
metabolite] Dose***
Clobazam Frisium, Urbanol 8-60 hours anxiolytic, 20 mg anticonvulsant DMCM ? ? anxiogenic, N/A, not used convulsant therapeutically Flumazenil Anexate, Lanexat, 1 hour antidote N/A, typical dose Mazicon, Romazicon 0.2 - 0.6 mg Eszopiclone**** Lunesta 6 hours hypnotic 3 mg Zaleplon**** Sonata, Stamoc 2 hours hypnotic 20 mg Zolpidem**** Nytamel, Stilnoct, 2 hours hypnotic 20 mg Stilnox, Zoldem, Zolnod Imovane, Rhovane, Zopiclone**** Zileze; Zimoclone; 2 hours hypnotic 15 mg Zimovane; Zopitan;
Zorclone, Nonbenzodiazepine = Imidazopyridines o zolpidem (Ambien) o alpidem o saripidem o necopidem = Pyrazolopyrimidines o zaleplon (Sonata) o indiplon o ocinaplon = Cyclopyrrolones o eszopiclone (Lunesta) o zopiclone (Imovane) o pagoclone o suriclone o pazinaclone o suproclone = Other structural families o Etffoxine o Panadiplon o CL-218,872 o SX-3228 o L-838,417 o RWJ-51204 o Y-23684 Growth hormone (GH) also has been identified as an oocyte survival factor acting secondarily to the IGF-I pathway. Gonadotropins or growth hormones may act to promote follicle survival, in part, by altering follicular steroidogenesis (eg. human preovulatory follicles cultured in the presence of IGF-I produced more estrogen and less progesterone.

Studies conducted on preovulatory follicles suggest that, without supportive hormones such as human chorionic gonadotropin (hCG), insulin-like growth factor-I (IGF-I), and human follicle stimulating hormone (hFSH), cultured follicle cells will undergo apoptosis (previous studies have shown). Growth hormone (GH) also has been identified as an oocyte survival factor acting secondarily to the IGF-I pathway (previous studies). In some cases, there is evidence that the various gonadotropins or growth hormones may act to promote follicle survival, in part, by altering follicular steroidogenesis. For example, human preovulatory follicles cultured in the presence of IGF-I produced more estrogen and less progesterone (shown by previous studies).
In agreement with my belief that timing/concentration (leading to - response with sexual stimulation; intensity - due the levels of (various environmental cues) change in one direction and durations versus the cue's levels of change opposite in the other direction to create cycles (any and all lengths; seasonal; annual), that the fish (any and all life), naturally take in their sexual response via genetics and/or entrainment to these environmental cues (including maturation (annual cycles and the duration and rate and levels of environmental cues - some hormonal factors such hCG, IGF-I, hFSH, GnRH) - melatonin inhibitor) and seasonal-hormonal responses impact on the willingness of fish and any and all life to spawn/mate/breed/have sex) in response to environmental cues that cause late-cycle high of plasma E2 and testosterone highs may be the cause in feedback to the brain (hypothalamus, and pituitary glands), inducing synthesis and secretion of LH at the expense of FSH production. It is believed that (estradiol-17) E2 is produced in the granulosa cells by the action of the aromatase enzyme and converts testosterone to E2.
Circulating E2 and testosterone levels rise early in final maturation, while the oocyte germinal vesicle is still migrating; peak testosterone levels respond less behind peak E2 levels.
Androgen and estrogen levels both fall coincident with the peak in circulating E2 that is concurrent with GVBD (shown by previous studies) artificial induction can be accomplished using synthetic analogue of gonadotropinreleasing hormone (GnRHa). Noted that changes in circulating steroid hormones rise of internal LH in the blood plasma is induced by GnRHa (past studies).
These observations further suggest that rises in androgen and estrogen levels...
Fish have shown cyclic pattern of oogenesis and endocrine correlative cyclic pattern.
Responding to such environmental cues (eg. photothermal - diurnal and seasonal changes in water temperature and day length) and therefore reproducing in timing cycle that is entrained. Deposition of yolk granules in the growing oocytes is triggered by decreasing day length and water temperature (eg. monsoons and/or rainy season and other environmental factors caused by such seasons and the cues that belong to such seasonal changes that the fish type and entraining that they respond to). Furthermore these seasonal related cues come with a distinct rise in concentration of estradiol-17:1 (E2) and testosterone, as well as Vg.
Exogenous E2 may induce hepatic vitellogenesis suggesting its role as hormone controlling this process.

Oocyte growth begins at deposition of lipid droplets (made of wax esters, which are made from circulating triglyceride precursors) /yolk protein and yolk granules in the ooplasm. Past studies have shown that oocyte growth is correlated to yolk protein deposition in the yolk granule.

Ovarian follicles and vitellogenesis:

1. Theca is the outermost layer derived from stromal connective tissue and is made up of collagen fibers, fibroblasts, steroidogenic cells, and capillaries or other vascular components (according to past studies).
2. Germinal epithelium-derived follicle cells then flatten to form a singular granulosa cell layer under the basal lamina and on top of the cellular chorion or ZR.
3. Junctional complexes between the follicle cells allow for the extracellular passage of Vg, through the theca, basement membrane, granulosa cell layer, and ultimately to the oocyte surface.
4. The ZR then takes on a perforated form by a process known as microvillar, which emanate from both the oocyte and granulosa cells (also based on past studies).
Re: Krill (Zoo Plankton) Farming In addition to any and all oily fish sued to produce healthy fish oils...
especially we are mass producing krill and shrimp...

For their omega-3 fats, eicosapentanoic acid (EPA) and docosahexanoic acid (DHA), but hooked together in a different form...

Fish oil is extracted from, omega-3 fatty acids are found in the triglyceride form, while krill/shrimp oil are hooked up in a double chain phospholipid structure.

The EPA leg phospholipid has a molecule known as astaxanthin, which is a very potent anti-oxidant. The phospholipid EPA and DHA in krill oil are more absorbable. Also Krill/shrimp oil enters more easily into the mitochondria and the nucleus membrane. Furthermore EPA and DHA from krill oil's phospholipid have more complex profile such as phosphatidylcholine, whose effect is reductive-stress-reducing choline, and also a natural emulsifier.

Krill oil has vitamin E, vitamin A, vitamin D and canthaxanthin, like astaxanthin. The anti-oxidant potency of krill oil is 48 times more potent than fish oil. Aparently it has 10 times more antioxidant activity than beta-carotene and as much as 1,000 times more than vitamin E.
Interestingly it is a source of alpha-tocopherol (vitamin E) and a derivative, called marine-derived tocopherol, that has antioxidant properties than alpha-tocopherol. It is postulated that tocopherols enable krill survive in the cold waters. As well they have the flavonoid luteolin. If this theory is correct it may be the only non-plant version of the substance... One claim of omega-3 of krill oil is that the phospholipid content of krill oil plays a role in omega-3 passing through the blood-brain barrier.

If we can mass produce the krill/shrimp cheaply enough they are a rich fertilizer, like fish fertilizer...

Astaxanthin is used as a feed supplement for salmon, crabs, shrimp, chickens and egg production. Regardless of the source, astaxanthin provides some important benefits beyond coloration. It also has been found to be essential for proper growth and survival.

Vitamin A
Krill Oil Phospholipids, Omega-3 rich Eicosapentaenoic Acid (EPA) Docosahexaenoic Acid (DHA) Omega-6 Fatty Acids Astaxanthin Transfat Malacostraca include:

crabs, krill, pill bugs, shrimp, and relatives Kingdom: Animalia Phylum: Arthropoda Class: Malacostraca Members of this Class UV before and/or after freezing, thaw in water prior to injection and/or freeze dry.

A number of studies have shown that krill oil is very effective in: reducing LDL-cholesterol, raising HDL-cholesterol therefore cardiovascular disease; Lowering blood sugar threfore diabetes; Treating the pain; Inflammation from rheumatoid arthritis (therefore see list of inflamatory diseases pages 23 to 25) and Aches and pains (therefore as an pain releiver eg.
substitite for tylenol/aspirin...), PMS and dysmenorrhea, adult ADHD; Healthy brain and nervous system neurodegenerative diseases and It also crosses the blood-brain barrier, which makes it available to the eye, brain and central nervous system to alleviate oxidative stress that contributes to ocular, and neurodegenerative diseases such as glaucoma and Alzheimer's;
Healthy liver function; Improved immune function; Concentration and memory;
Healthy joints.
Astaxanthin is potent antioxidant, it may be beneficial in cardiovascular, immune, inflammatory and neurodegenerative diseases. Research supports the assumption that it protects body tissues from oxidative damage.

Cod Liver versus Fish Oils are: More efficiently and more easily absorbed;
Superior antioxidant protection; Can be renewable; Does not oxidize.

Re: Any and All Shrimp especially Krill Maturation and Spawning We plan to use all the aquaculture (fish, crayfish/crawdads - arthropods, amphbians, any and all life) environmental (eg. page 1 point 6. and Re: Growth and Maturation and Re; Spawning) including temperature/PH/DH and any and all environmental conditions shock, filtration of seawater and/or (eg. simulate annual seasons - including monsoon season); most important is photoperiod (melatonin might be used to alternate use during winter and non-use during the longer lighting summer) length of daylight and (also variation in intensity and/or light pulses) relative to the length of darkness), proper stimulation olfactory organs, FEO
(food entrained oscillator... proper stimulation of taste buds, food availability; water temperature; water flow rate (activity - possible impacts on metabolism )(velocity m/sec - flooding and water currents);
pH/DH (water hardness); Dissolved Oxygen (mg/I); Free carbon-dioxide (mg/I);
Total alkalinity (mg/i); Chlorides (mg/I); TDS (mg/I); DOM (mg/I); Specific conductivity (mhos)/25C, some believe in the cycles of the moon, atmospheric pressure, availability and desired quality types based on the fish type of spawning substrate, availability of aquatic plants, sticks, gravel, and safe nooks and crannies, nutrition, and the presence of other fish.. .The effects of these external factors on the blood concentrations of substances (eg. exercise and activity and changes in PH/DH ...and/or melatonin antagonists) could be used together with GnRH, gonadotrophin, vitamin A (beta carotene), retinoic acid, for maturing the gametes which is absolutely necessary for spawning/mating/sexual intercourse/breeding to result in healthy embryos (mitosis/meiosis)...

We plan to mass produce the krill using the above breeding/spawning techniques.

One spawning method is shrimp eyestalk ablation.

We could try to grow (regenerate) the bioreactors (proliferate via ECM and/or HOX), we could even slice the krill to a survivable cellular colonies (maybe with a threshing machine with very small and fine and sharp blades) and regrow them via regeneration ECM/HOX and other technologies Gerard Voon has patented, whereby the cells are constantly renewed as an altenative artificial maturation and spawning techniques covered in this patent. (Jeff 30 of 35%) Krill can be harvested and processed for their protein (40% or more of dry weight) and lipids (about 20% in E. superba). Their exoskeleton for chitin, hydrolytic enzymes such as proteases, carbohydrases, nucleases and phospholipases, found in their digestive gland in the cephalothorax of the krill; these enzymes might be usable in producing biomass, bio fuel and bio crops ethanol...

Other uses include krill pastes or processed krill as food additives, e.g. in the form of krill oil gel capsules.

Krill are used as pastes, for food addictives oil gel in capsules enzymes, necrotic tissue and chemonucleolytic agents.

Their oils can be used as biofuel...
Algae Algae belong to a group of micro organisms known as protists. Crypthecodinium cohnil and Schizochytrium are two strains/varieties of algae currently employed for supplements of omega-3. They are proliferated in fermenters under specific environmental conditions. When ready the algae are dried and oil separated. By creating a closed system oil from the algae is contaminants free and monoculture.

Fish oil or krill oil, both have EPA and DHA, algae has the DHA. The organisms that feed on the algae retroconvert some DHA back to EPA, although more direct EPA is often needed...
However, research suggests that DHA may be the more critical of the two omega-3s for certain health benefits, especially for infants.

DHA from algae was more positive in its effects on the growth of colon cancer cells in mice than EPA, 90 percent less tumor growth. Reducing platelet aggregation or lowering blood cholesterol as combinations of EPA and DHA from fish oils. Depression may also be associated with reduced blood levels of omega-3 fatty acids and or Omega-6...
Phytoplankton Krill probably react (spawn/sexually triggered/mate/breed in response regulated to the environmental sensitivities in height and duration to the developing cycle of) the Pacific upwelling of cool waters in spring that supports the (especially sensitive to availability - eg.
feeding food frenzy of clouds of phytoplankton) and nutritional and/or odour and/or taste and/or energy associated with phytoplankton bloom. Therefore the timing (entrained, genetically versus glandular recall) bloom is linked with the hatching of krill eggs.

Re: Micro organisms Including Diatoms And Phytoplankton Have the Following Benefits to the Life that Feed on It (Shrimp and/or Krill above Especially feed on Phytoplanktoon).
= immune system enhancement = general nutrition - provide ultra-potent lipids to enhance brain function = energy - increases energy and vitality = promotes better sleep - more restful and restorative = antioxidant protection from cancers and degenerative diseases = cardiovascular health - supports a healthy heart = blood pressure control = cholesterol reduction = liver health - supports a healthy liver = neurological support - mental alertness, ADHD, Parkinsons, and general dementia = alkalizing - the balancing of body pH away from unhealthy excess acidicity = anti-inflammatory effects on membranes - promotes relief from joint pain = cell wall improvement through increased permeability and flexibility = detoxification and cleansing - supports removal of toxins from cells and organs = skin care - such as acne, psoriasis, dermatitis = better vision - more effective than Lutein = blood sugars - stabilizes blood sugar levels (aids those who are diabetic or hyperglycemic) = supports weight loss Re: Factors that Diatoms/Phytoplankton/Plankyton/Zooplankyton/Micro Organisms that are Needed for them to Thrive Most needed seems to be iron... others include temperature, salanity, transmissivity (suspended particulates), oxygen, nitrate, phosphate, silicate, CO2 partial pressure, PH, iron binding ligands...All plants benefit from active nitrate reductase, and the ability for nitrogen fixation makes them good to fertilize hydroponic waters...

The most proliferate are the heterotrophic bacteria, ciliates, and flagellates... We are studying their genes 1. to determine the genes responsible for their rapid proliferation, to insert these genes into cow/pig/chicken... via ovary/testes precusor and gamete cells to mass produce their meat, and/or we could insert these genes responsible for rapid proliferating into micro organisms that have strong enzymes (eg. from termites) for ethanol production, perhaps cutting the processing time and making the process easier. On the other hand we could insert DNA
and/or plasmid into and either pre genetically altered such as enucleated to be compatible with human DNA/plasmid (so these micro organisms: heterotrophic bacteria, ciliates, and flagellates) can reproduce with eg. human DNA, for future extarction into stem cells seed DNA
and/or any other uses for any and all life DNA... These procedures with these micro organisms, can also be used with nitrogen fixing with healthy fertility increasing microorganisms in soil, compost and/or mushrooms, any and all life (especially cells) that we want to mass produce rapidly (harnesssing the rapid rate of proliferation of these heterotrophic bacteria, ciliates, and flagellates)...

Diatoms Include = Diatom A cont. C cont.

A = Attheya longicornis = Chaetocerotaceae = Attheya septentrionalis = Clepsydra (genus) = Asterionella = Auxospore = Coscinodiscineae = Attheya = Attheya arenicola B D
= Attheya armata = Attheya decora = Bacteriastrum = Didymosphenia geminata = Attheya flexuosa = Biddulphiineae = Attheya gaussii F
C
= Frustule = Centrales = Chaetoceros P
= Chaetoceros diadema = Chaetoceros furcellatus = Pennales = Phaeodactylum tricornutum Pinnularia T

= Thalassiosira pseudonana We can collect diatoms resting spores shallower floors/beds of water...
scooping the resting spores versus trying to reach them in deeper waters. We will grow them including krill by putting silicon, nitrogen, phosphorus and iron and adjusting temperatures - we could grow them with hay fusion, filamentous algae, cladophora, vaucheria ... also dphania and brine shrimp. When it comes time for the Krill to spawn we control the the conditional factors including providing an abundance of food and we move them to a deeper pen...

Re: Easy to Grow Sources of Protein Supplements 1. Proteins extracted from Earth Worms and/or grubs - freeze dried/powdered.
2. Proteins extracted from ants, crushed/pulverized (their exoskeleton perhaps dissolved in acid and/or enzymes as long as the method of removing the exoskeleton - if need be at all does devalue the protein desired) frozen and/or freeze dried.
3. These ants and eartheworms can be fed sugar and/or restaurant waste (shredded).
4. They can all be treated to UV then (eg. flash frozen) frozen and then UV
again then thawed and fed as protein.
5. We could add algae taste and/or smell to make the above sources of food more appetizing (pellets).

Since these sources of protein are so cheap, as an alternate for human supplements/additive (pills/capsules/powder), for animal feed and/or any and all fish feed.
And or pwdered and fed to animal meat in bioreactors to mass produce mass amounts of meat cheaply, or as fertilizer and also to gro fat cells (from eg. seals, whales and walruses) to produce biofuel and also a delicacy (GP 0.5%).

Re: Micro organisms Lacto Bacillius and/or Actinomycetes, diatoms... both of which are photosynthetic bacterias -which can be used for pre digestion of feed for any and all creatures - making the food more nutritional. Lacto Bacillus also produces lactic acid from sugars, carbohydrates and yeast (which sterilizes - we could produce antibacterial hand washes/antibacterial wet tissues/gels... lactic acid could also possibly mixed with chitin and/or furanone - anti agent used against harmful organisms), lactic acid also ferments and decomposes lignin and cellulose, which can be used for any and all biocrops to turn into ethanol.

Re: Biofiims/Quorum Sensing - believed to be sensed via pheromone and/or autoinducers by secreting signal molecules; cell to cell signaling We could possibly use genes that govern high virulence (eg. E.Coli)and genetically Modify into cows/pigs/chickens/fish meats such that we can clone fast growing life forms but also the meats for food. Also we could use biofilm/quorum sensing for yeast, bacteria in cow' s mouth (that produces methane - for fuel), micro organisms found in a cow's/horses'/
gut for cellulose (eg. hay) processing - fermentation (and their enzymes), micro organisms for compost, sewage, manure, restaurant waste, possibly garbage waste; by adding sugar we turn soils fertile ... these materials can be (thoroughly/evenly/strategically) mixed with sugar/carbohydrates/starch is such a way that the clusters of waste that are easier to breakdown need less sugar, while the clusters of waste that are harder to breakdown need the supplement of higher concentration of sugar/carbohydrates/starch to give the micro organisms energy and vitality together with adequate biofilm/quorum sensing for the micro organisms to flourish.
The following (below) suggests that there are many factors affecting micro organism in the blood stream or occupying infected cells, actually some similarity to fish (any and all life) and their responses to contents (and concentration) and conditions in the water (medium)...We are using melatonin antagonist for micro organisms that are light sensitive, we also will control any and all environment factors (the same as in fish maturation) in cycles (tall rise and fall of and timing that cause cycle and durational changes), as well as the hypothalamus and pituitary gland hormones (and all their analogues) as well as LH... We could also try viagra, cialis, LHRHa (with dopamine agonist), Beta Carotene, (Vitamin A), retinoic acid, water melon rind, Kapok (Ceiba pentandra) bark....

Re: Regulation of virulence (Cited Prof. Dr. Hubert Hilbi, SSO4) - Most of which is well known and widely available in other Studies = DNA structure and transcription factors = Environment - temperature, pH, ions, osmolarity, oxygen, nutrients = Host cells - contact - intracellular environment = Cell density (quorum sensing) - autoinducer systems - processes (bioluminescence, biofilm, virulence) = Genome structure and topology = Environment - temperature, growth phase, pH, ions, osmolarity, oxygen, nutrients = Host cells - secretion-dependent regulation - intracellular environment (macrophages, amoebae) = Cell density (quorum sensing) - autoinducer systems - processes (bioluminescence, virulence, biofilm) Ceiba pentandra bark is believed to be diuretic, aphrodisiac, and to treat headache, as well as type II diabetes. We could test the effectiveness and isolate the active ingredient then commercially make the active ingredient(s) available.

Re: Photoperiod and the Natural Production and Injested Vitamin D

Tissues in the body convert Vitamin (via receptors and enzymes), from Vitamin D; 25-hydroxyvitamin D and/or 25(OH) into active ; 1,25-dihydroxyvitamin D.

Vitamin D interacts with 200 genes.

We are particularity interested in smooth muscle growth (as a perhaps cyclical response in coordination with the seasonal light and dark photoperiods) - thus the level, timing of change, steadyness (not too volatile versus times when is needs to be volatile to induce the eg. fish (any and all life)), of rate of change the duration it prolongs the high and lows in mimicing or even find a cyclical combination that works even better for sexual maturation and/or spawning/breeding/sex/mating ... if the postulation in the paragraph below is correct than it might help any and all tissue not only including the testes and the ovaries but all life and their body parts, including stem cells in Gerard Voon's bioreactor ... Perhaps the ability of Vitamin D
to help those with dysfunctioning Seasonal Affective Disorder (SAD) may help growth, sexual maturation and sex/mating/breeding/spawning...

An interesting postulation is that Vitamin D may be released naturally into the organism during Photoperiod (response to sun light), and therefore may play an opposite role than melatonin (released in darkness). Thus Vitamin D may play a role in sexual maturity and growth...

We testing the effectiveness of Vitamin D on any and all (anti inflammatory) of the below diseases:

Cardiovascular Blood Pressure Multiple Sclerosis (MS) Rheumatoid Arthritis (RA) Inflammatory Bowel Disease (IBD) Interstitial Cystitis (IC) Fibromyalgia (FM) Autonomic nervous dysfunction (AND neural-mediated hypotension);
Pyoderma Gangrenosum (PG) Chronic Fatigue (CF) and Chronic Fatigue Syndrome (CFS).
Chronic hepatitis Systemic lupus erythematosus Arthritis Thyroidosis Scleroderma Diabetes mellitus Graves' disease Beschet's disease and Graft versus host disease (graft rejection).
Chronic inflammatory pathologies such as aneurysms Hemorrhoids Sarcoidosis Chronic inflammatory bowel disease Ulcerative colitis Crohn's disease and vascular inflammatory pathologies Disseminated intravascular coagulation Atherosclerosis Kawasaki's pathology Coronary artery disease Hypertension Stroke Asthma Chronic hepatitis Multiple sclerosis Peripheral neuropathy Chronic or recurrent sore throat Laryngitis Tracheobronchitis Chronic vascular headaches (including migraines Cluster headaches and tension headaches) and pneumonia Neurodegenerative diseases including Demyelinating diseasessuch as multiple sclerosis and acute transverse myelitis;
Extrapyramidal and cerebellar disorders such as lesions of the corticospinal system;
Disorders of the basal ganglia or cerebellar disorders;
Hyperkinetic movement disorders such as Huntington's Chorea and senile chorea;
Drug-induced movement disorders such as those induced by drugs which block CNS
dopamine receptors;
Hypokinetic movement disorders such as Parkinson's disease;
Progressive supranucleo palsy;
Cerebellar and Spinocerebellar Disorders such as astructural lesions of the cerebellum;
Spinocerebellar degenerations (spinal ataxia) Friedreich's ataxia Cerebellar cortical degenerations Multiple systems degenerations (MencelDejerine-Thomas Shi-Drager and Machado Joseph)); and systemic disorders (Refsum's disease Abetalipoprotemia, ataxia telangiectasia and mitochondrial multi-system disorder);
Demyelinating core disorders such as:
Multiple sclerosis Acute transverse myelitis;
Disorders of the motor unit such as neurogenic muscular atrophies (anterior horn cell degeneration) such as Amyotrophic lateral sclerosis Infantile spinal muscular atrophy and juvenile spinal muscular atrophy);
Alzheimer's disease;
Down's Syndrome in middle age;
Diffuse Lewy body disease; Senile Dementia of Lewy body type;
Wemicke-Korsakoff syndrome;
Chronic alcoholism;
Creutzfeldt-Jakob disease;
Subacute sclerosing panencephalitis Hallerrorden-Spatz disease; and Dementia pugilistica Malignant pathologies involving tumors or other malignancies such as:
Leukemias (acute chronic myelocytic chronic lymphocytic and/or myelodyspastic syndrome);
Lymphomas (Hodgkin's and non-Hodgkin's lymphomas such as malignant lymphomas (Burkitt's lymphoma or Mycosis fungoides));
Carcinomas (such as colon carcinoma) and metastases thereof;
Cancer-related angiogenesis;
Infantile hemangiomas;
Alcohol-induced hepatitis.
Ocular neovascularization Psoriasis Duodenal ulcers Other diseases that we are testing for effectiveness include parkinsons, diabetes, osteoporosis, multiple sclerosis, breast cancer, colon cancer, prostate cancer, autoimmune disease, cardiovascular (blood clotting - posssibly in gel, powder or imbedded in gauze (perhaps with antibacterials such as furanone and/or chitin) to an open wound to prevent bleeding to death and then apply a layer of ECM/HOX for regeneration assuming that the wound is not too severe - in which case it may be better to grow an entire new limb using the ECM scaffolding and stem cells of a recently deceased body part donor disease), any and all sexually transmitted diseases, herpes... hepatitus, skin problems eg. serious acne, any and all skin conditions, depression, schizophrenia, seasonal affective disorder, peripheral artery disease, tuberculosis, cancer, periodontal disease, multiple sclerosis,...

Using all our Gerard Voon's patented inventions (Methods and Techniques, Models) Titled:
BioScience and all the above: Fish liver oils, including cod liver oil, Fatty fish species, Herring, Catfish, Salmon, cooked, Mackerel, Sardines, canned in oil, Tuna, canned in oil, Eel, One whole egg (perhaps more duck eggs because of their oiliness).

EPA healthy heart - healthy body Promotes a healthy heart and circulatory system Supports healthy homocysteine levels Supports proper immune function Promotes good mood and emotional well-being Supports joint flexibility DHA healthy mood - healthy mind Essential for memory, cognitive function, 'learning and focus Supports a healthy pregnancy Promotes good mood and emotional well-being Reduces harmful effects of stress Supports the visual and brain development of fetus' and infants GIA healthy skin & hair - healthy hormones Promotes beneficial prostaglandins that maintain hormonal balance Provides a "feel good" effect, improves mood Nourishes hair and skin Supports joint flexibility Promotes normal body fat metabolism Vitamin D Receptor will be used to help us in cell proliferation and/or differentiation (from stem cells to any and all life as well as micro organisms). We will also use its properties via Gerard Voon's patented stem cells to immune cells (adjuvant and immune cells priming as a serum for diseses with antigens or unchanging intracellular proteins - for harmful diseases that mutate their cellular antigens on their membranes; or just fortify the immune response during disease out break), Vitamin D is also involved in white blood cells eg. monocytes and activated T and B
cells.

Re: Waterboume mirco organisms (eg. diatoms) could be added with sugar (in its various forms).. .white rice... to Hydronics ... and any and all mediums.

The waterbourne micro organisms (eg. diatoms), can break down ammonia/nitrates/urea/sugars/carbohydrates/sewage/detritus/hay fusion/compost/tea manure... in the hydroponic media... to make the water fertile for plants and algae...

Re: Drawing 1. - Tall Greenhouse Solar and Mirror Invention 1. Solar Panels on the roof of the Greenhouse.
2. Mirrors on the bottom ceiling to catch reflected light from angled mirrors surrounding th greenhouse.
3. The greenhouse plants space.
4. The mirrors angled to aim sun's light at the mirrors in the ceiling (possibly parabolic).
The reason the greenhouse is so tall is to allow surrounding mirrors further out to also aim and reach the ceiling...

Re: Cure for Baldness and Fur Industry We take a newly deceased donor, we take off the scalp (and follicular skin below) hair and/or fur and all and treat its raw side with ECM/HOX (Jeff 20% of 35%) any and all proliferating factors and/or flush the skin/flesh under the hair/fur with deterregent and until only the ECM
sccafold remains add stem cells from the own patient (to avoid rejection) (to grow a whole new scalp with hair/fur or in the case of fur, we could cut off the top skin for fur/skin... (also aligators/crocodiles and/or eels...) while leaving the bottom layer of follicle base behind that we expose ECM/HOX (Jeff 10% of 35%) and/or any and all proliferation factors to regenerate the fur for human bald patients we then operate on the patient to either descalp and/or dermabrasion the patient's upper layer of then attach the new scalp to the bald patient.

Claims (4)

Claims The primary objectives of this invention is to mass produce (gametes) eggs (especially)/sperm for initiation of stem cells sources and artificial fertilization and in vitro surrogate implantation.
This technology can be used an any and all life forms (sometimes using cross sources of hormones and or hormone analogs)...

The reason we want to produce these substances extemally, outside of the body is because it is invasive to remove (eg. oocytes/eggs) from the female's ovaries versus mass production of multiple external ovaries.

We need ovarian and testicular tissue. And we need to mature the tissue.

1. We could remove a sample of ovarian and testicular tissue and regenerate to its full normal size by exposing it to HOX and ECM (Extra Cellular Matrix) .
2. We could take an ovaries and testes from recently deceased donors and wash with detergent and use the Extra Cellular Matrix Skeleton/Scaffold and repopulate with stem cells and converted stem cells into differentiated cells that normally belong in the specific places. ECM and Hyaluronic Acid and Hyaluronan and hyaluronate (Has1, Has2, Has3) and its fibronectin-type (collagen and also laminin) components together with HOX can be used to regenerate and grow any and all life forms. Hyaluronic also supports virulence and could be used for further augmenting the sexual maturity and successful fertilization of healthy in vitro any and all progeny...
3. Alternate method of proliferating any and all life. External cues originating outside the cell, (eg epidermal growth factor (EGF)), induce proliferative signaling via beta-catenin, an inside the cell protein is necessary in cell adhesion at the plasma membrane and transcription of cell cycle genes in the nucleus (especially epithelial cells). The factor Wnt 3a, is an agonist of beta-catenin transcriptional activity, that induces greater signaling than either of the ligands (EGF and Wnt 3a) alone. However each uses a different sub-cellular pool of beta-catenin. WNT signaling with either Wnt3a, LiCl (causes the accumulation of beta-catenin and also is a dopamine antagonist, see Re:
Induced Spawning - section below - GnRH, Gonadotrophin, and other hormones...
), or the constitutively active S33Y mutant of .beta.-catenin. WNT factors and .beta.-catenin also affect lymphocyte progenitor fate as well as stem cell self-renewal Stimulation with LiCl markedly increased the amounts of total and nonphosphoryiated .beta.-catenin in the myeloma cell lines tested.
4. Another option is with antigenic peptide p33 and a weaker agonist A4Y
ligand and mixed with LiCI or KCI, and pulsed with [ H]thymidine to proliferate all life (including human stem cells). Also IL-2 production had a large increase because of the lithium, as measured by proliferation of the IL-2-dependent, it has been postulated that regulates the T lymphocyte responses. GSK-3 phosphorylates NF-Atc (nuclear factor of activated T cells (NFAT)), which promotes cytoplasmic localization and also export from the nucleus. Therefore, the presence of LiCi should inactivate GSK-3, resulting in prolonged dephosphorylation of NF-ATc and sustained nuclear localization. GSK-plays an important role in antigen-specific T cell activation by regulating NF-ATc localization.
5. Once the stem cells or any other tissue proliferated using this method is ready to be grafted back into a patient's body (eg. after regenerating the body part), we can use a beta-catenin antagonist protein E-cadherin to treat the cells to avert cancer...
6. Both 1. and 2. and 3. and 4. are grown outside the body - extemal ovaries/testicular organs (the result is a regenerated extemal (once fully regenerated, mature ovary/testes), they can be used as a sort of egg and sperm mass production factories ... these can be grown in Gerard Voon's patented bioreactor that uses synthetic blood to bring oxygen and remove CO2 from the tissue/body part and either dialysis and/or functional external from the body kidney that filters waste that is also via the bioreactor filters the blood and uses it for its own survival as well; at the other end nutrients and vitamins, growth hormones and supplements are added based on the stage of growth...of the best recipe. In addition to ovarian and testes tissue the above techniques can be used for any and all body parts (from healthy pancreas cells (reinjected - possibly differentiated from stem precusor cells) to full regenerated pancreas to naturally produce insulin for transplant back into the patient's body (Dad)), also immune cells... glands, organs and tissues. Other uses include regenerating and culturing swallows salivary glands and solving the cues, environmental and/or hormonal cues...any and all cues that trigger swallows to make bird's nest soup...also we could regenerate Mako shark's fins separate from the body, possibly from stem cells... In the case (of growing/differentiating ovarian/testes tissue from stem cells) we need to mature the ovaries/testicles ... There are both environmental and internal factors (for water bourne creatures) that are involved and even necessary for maturation ... there are environmental factors include: (eg. simulate annual seasons - including monsoon season); most important is photoperiod (melatonin might be used to alternate use during winter and non-use during the longer lighting summer) length of daylight and (also variation in intensity and/or light pulses) relative to the length of darkness), proper stimulation olfactory organs, FEO (food entrained oscillator... proper stimulation of taste buds, food availability; water temperature; water flow rate (activity -possible impacts on metabolism )(velocity m/sec - flooding and water currents); pH/DH (water hardness);
Dissolved Oxygen (mg/l); Free carbon-dioxide (mg/l); Total alkalinity (mg/l);
Chlorides (mg/l); TDS (mg/l); DOM (mg/l); Specific conductivity (mhos)/25C, some believe in the cycles of the moon, atmospheric pressure, availability and desired quality types based on the fish type of spawning substrate, availability of aquatic plants, sticks, gravel, and safe nooks and crannies, nutrition, and the presence of other fish...The effects of these external factors on the blood concentrations of substances (eg. exercise and activity and changes in PH/DH ... and/or melatonin antagonists) could be used together with GnRH, gonadotrophin, vitamin A (beta carotene), retinoic acid, for maturing the gametes which is absolutely necessary for spawning/mating/sexual intercourse/breeding to result in healthy embryos (mitosis/meiosis)...
7. Responses by gonadotrophin induced cycles, for females (vary) (some ovulating 70 eggs), we need alleles to induce the follicles to mature, it seems follicular fluid from large follicles (1-pFF), to mature oocyte cytoplasmic and nuclear maturation... It has been hypothesized that Alleles contributing to induction of follicle maturation and steriodogenesis, that (whereby) the maturation process is subject to gonadotropin inducing cycles, contribute to under environmental factors, to mature follicles, to produce estrogens to create and LH surge and thus ovulating.
8. We could also use ovarian tissue and/or full ovaries to produce eggs such as sturgeon (caviar) and turtle and/or crab eggs...this technology could be used to produce any and all eggs...

We can also use synthetic meiosis-activating sterol, derived from follicular fluid (FF-MAS).
Re: Inducing Maturity of Gametes from Ovarian and Testicular Tissue Vitamin A in the form of beta-carotene (BC) an also retinol ester, is supplied to oocytes and cumulus-granulosa via the ovarian follicles (by binding with molecules).
Vitamin A, retinoic acid are involved artificial insemination, and in artificial reproductive techniques (ART), eg.
superovulation, ovum pick up, and in vitro maturation culture. RA is possibly involved in affecting cytoplasmic maturation through gene expression of gonadotrophin receptors, midkine, cyclooxygenase-2, and nitric oxide synthase in cumulus-granulosa cells, oocytes, blastocyst, follicles, gametes (any and all ovarian and/or testes cells).

Tissues needed for the hormonal cascade are the hypothalamus, pituitary gland, and gonads...
The cascade of hormones are gonadotropin releasing hormones (GnRH), which travel from the hypothalamus to the pituitary gland...The pituitary regulates growth and reproduction (we could also use it to further enhance stem cell proliferation). Specialized pituitary cells are signalled by GnRH to release gonadotropic hormones. These gonadotropic hormones affect the gonads, that then produce steroids that regulates final maturation of the gametes.

Maturation of the egg includes vitellogenesis, in this process the yolk proteins (we could grow fully complete livers (deceased donor, detergent and ECM matrix with HOX
and/or stem cells and/or specialized cells per portion of belong on the matrix) outside the body and produce (in Gerard Voon's patented bioreactors)) externally produced yolk protein (mass production) are produced in the liver, transport it to the ovary, to produce much egg size.
The yolk is necessary for the embryo growth.

We are studying the induction and its contribution of Germinal vesicle migration and germinal vesicle breakdown (GVBD) to the gametes development.

Re: Next Stage After Gametes have Matured At this stage (when the gametes have matured) prostaglandins are produced.
They induce ovulation, rupturing of the follicle cells holding the egg. The egg makes its way to the body cavity or ovarian lumen... Interestingly after ovulation, the health of eggs can decrease.

Re: Induced Spawning In addition to viagra, cialis, water melon rind, Kapok (Ceiba pentandra) bark We can induce spawning by extracting hCG from the urine of pregnant (large sources that are easy to grow and breed) - any and all sources eg.
cows/pigs/horses/dogs/cats...

Major methods include injection of a GnRH analogue; agonist (Leutinizing Hormone Releasing Hormone, LHRHa) with dopamine possible antagonist:

Dopamine blockers and depletors include:
.cndot. metoclopramide .cndot. pimozide (Orap) .cndot. haloperidol (Haldol) .cndot. olanzapine (Zyprexa) .cndot. tetrabenazine .cndot. chlorpromazine hydrochloride (Thorazine) .cndot. domperidone .cndot. droperidol (Inapsine) .cndot. fluphenazine hydrochloride (Prolixin) .cndot. metoclopramide (Regian) .cndot. perphenazine (Trilafon) .cndot. prochlorperazine (Compazine) .cndot. thiethylperazine (Torecan) .cndot. trimethobenzamide (Tigan) .cndot. reserpine (Serpalan) - the fish may be reacting to a change in serotonin level and injection of gonadotropin and hCG (Human Chorionic Gonadotropin), Ovopel, Ovaprim-C, Ovatide, Ovaplant, CPE
(Carp Pituitary Extract) - we also plan to use the above hormones to proliferate stem cells and/or specialized cell regeneration).

Genetic (chromosomal modification)...to induce polyploidy, gynogenesis and androgenesis can be used for all life (aiming to control sex, inhibit sexual maturation and rapid inbreeding -Thorgaard, 1986). Methods to include using temperature sock, high hydrostatic pressure and/or chemicals... on fertilized eggs. Usage of these methods at the final stages of meiosis will retain a second polar body resulting in triploids, furthermore later usage of such methods avoids first cleavage resulting in tetraploidy...

Irradiating the sperm results in gynogenic progeny (progeny having only mother's genes) ... Irradiating pre fertilized eggs and then fertilizing results in androgenic progeny (progeny having only the father's genes)... Preventing first cell cleavage restores diploidy.
Methods used can obtain all-female population and/or all0female triploid populations. Start with gynogenic progeny...hormones are used to reverse the sex of the population to phenotypes of males but genetically female. These quasi-males fertilize normal female eggs, that produce all female progeny, the goal is get rid of inbreeding via gynogenic males are prevented.

These methods can be used to produce (via sample from live donor to regenerate - including stem cells - into full matured) ovarian and testicular tissue (and/or collect gametes directly from recently deceased donors) to use in in vitro fertilization.

Re: Artificial Water Bourne creatures' Gills By growing (via stem cells and/or regeneration/cloning of the body parts) or removing gills from the most suitable fish...we could filter out oxygen and release carbon dioxide for divers, submarines, underwater stations and/or use the carbon dioxide for any and all algae and plant growth.

Re: Melatonin Antagonist for growth and/or sexual maturity Bright light therapy is a popular method which prevents the conversion of serotonin to melatonin. The same results from more time in natural sunlight.

Since extending photoperiod is the primary means of inducing fish to mature and spawn (life cycles), and melatonin (produced by the pineal gland during darkness in the environment - the result of our visual receptors) is known to reduce rate of growth and sexual maturity therefore we are using melatonin antagonists including: muscarinic cholinergic antagonist;
mecamylamine, a nicotinic cholinergic antagonist; atenolol, a .beta.-adrenergic antagonist;
phentolamine, a nonselective a-adrenergic antagonist; prazosin, a selective a-adrenergic antagonist; haloperidol, a dopaminergic antagonist; naloxone, an opioid antagonist; and ibuprofen, a cyclooxygenase inhibitor. The following are theorized that hormones... are facilitated (some how involved) affected by the acetylcholine, dopamine, and/or prostagiandin.
Using (S20098: N-[2-(7-methoxy-l-naphthyl)ethyl]acetamide), and a Putative Antagonist (S20928: N-[2-(1-naphthyl)ethyl]cyclobutyl carboxamide to Block the Effects of Melatonin S20098, an agonist working on melatonin receptors. S20928 have both agonist/antagonist effects, at low applications is an antagonist on melatonin receptors in the suprachiasmatic nucleus and intergeniculate leaflet.

Further Melatonin Agonists and/or Antagonists that we are using to Pharmacologically Take the place of Photoperiod Inducing Growth and Sexual Maturity PREVIOUS NAMES Mel1 a Mel1b ML2 STRUCTURAL INFORMATION 350 aa (human) 363 aa (human) FULL AGONISTS Melatonin (M 5250) Melatonin (M 5250) 2-lodomelatonin (11899) 2-lodomelatonin (11899) 2-lodomelatonin (11899) 6-Chloromelatonin (C 0331) N-Propionyl melatonin N-Propionyl melatonin Melatonin (M 5250) N-Butanoyl melatonin N-Butanoyl melatonin N-Acetylserotonin (A 1824) 6-Chloromelatonin (C 0331) 6-Chloromelatonin (C 0331) 5-MCA-NAT
2-Methyl-6,7-dichloromelatonin 2-Methyl-6,7-dichloromelatonin (-)-AMMTC (-)-AMMTC
IIK7 (I 5531) PARTIAL AGONISTS 5-Methoxyluzindole 5-Methoxyluzindole Not known N-Acetyltryptamine (A 7342) N-Acetyltryptamine (A 7342) ANTAGONISTS Luzindole (L 2407) Luzindole (L 2407) Luzindole (L 2407) S20928 S20928 Prazosin (P 7791) 4P-PDOT N-Acetyltryptamine (A 7342) K185 (K 1888) SIGNAL TRANSDUCTION MECHANISMS Gi (cAMP modulation) Gi (cAMP modulation) Gq/11 (increase IP3/DAG) Gq/11 (increase IP3/DAG) cGMP modulation RADIOLIGANDS OF CHOICE 2-[1251]-lodomelatonin 2-[1251]-lodomelatonin 2-[1251]-lodomelatonin [3H]-Melatonin [3H]-Melatonin 2-[1251]-MCA-NAT
ABBREVIATIONS
4P-ADOT: 4-Phenyl-2-acetamidotetralin (-)-AMMTC: N-Acetyl-4-aminomethyl-6-methoxy-9-methyl-1,2,3,4-tetrahydrocarbazole 4P-CADOT: 4-Phenyl-2-chloroacetamidotetralin GR 196429: N-[2-[2,3,7,8-tetrahydro-1 H-furo(2,3-g)indol-1-yl]ethyl]acetamide IIK7: N-Butanoyl-2-(2-methoxy-6H-isoindolo[2,1-a]indole-11-yl)ethanamine K185: N-Butanoyl-2-(5,6,7-trihydro-11-methoxybenzo[3,4]cyclohept[2,1-a]indol-l3-yl)ethanamine Luzindole: 2-Benzyl-N-acetyltryptamine 5-MCA-NAT: 5-Methoxycarbonylamino-N-acetyltryptamine Melatonin: 5-Methoxy-N-acetyltryptamine 4P-PDOT: 4-Phenyl-2-propionamidotetralin 8M-PDOT: 8-Methoxy-2-propionamidotetralin S20098: N-[2-(7-Methoxy-l-naphthalenyl)ethyl]acetamide S20928: N-[2-Naphth-1-yl-ethyl]-cyclobutyl carboxamide Melatonin Receptors Re: Fish Blocking Ca2+ As Well, Vitamin D's Role in Ca2+ absorption Calcium was blocked by diluting water, removing calcium from the diet or by long term feeding of vitamin D-deficient diet. Melatonin production is affected by plasma Ca2+.
Another way to influence the effects of melatonin is via iontophoresis of the Ca2+ ionophore A-23187, prevents the inhibitory effect of both melatonin or Ca2+ antagonists...

Transcription factors called clock genes, which include Clock, period genes (per1, per2, and per3), cryptochromes (cry1 and cry2), and Bmall, regulate hypothalamic suprachiasmatic nucleus (SCN) and in peripheral tissues. The CLOCK and BMAL1 proteins initiate transcription of the period (per) and cryptochrome (cry) genes, and PER and CRY regulate self transcription through an interaction with the CLOCK/BMAL1 heterodimer to regulate cellular rhythms.

The SCN regylates rhythm in a several major body parts, including brain, pineal gland, adrenal liver, heart, kidney, muscle, pancreas, lung, and oviduct, through neural networks and hormonal excretion via the cerebrospinal fluid. It is clear that the same genes that are responsible for SCN rhythmicity are rhythmically expressed throughout the body.

Anyone who has night time cravings for food must wonder, there seems to be a clinging bond between the time, darkness (lack of activity) and other cues that motivates (possibly metabolism since an early dinner), you such as your rhythm has entrained you to crave food in the same daily rhythm ... This could be the cause of FEO (food entrained oscillator)...The FEO
is an alternate circadian clock acting on any and all living organisms, in addition to suprachiasmatic nuclei (SCN).

The necessary nutrients, include metabolites, ions, vitamins, nucleotides, and amino acids can also traverse from cumulus cells to the oocyte via GJC. Variations in these nutrients inputs can impact positively and/or negatively the developments from stage to stage needs of an ovary tissue into an embryo...

Measurement of carbohydrate concentrations can be taken via Glucose, pyruvate and lactate concentrations in FF, plasma and maturation medium.

Amino acid profiles (concentrations) can be Measured In follicular fluid (FF), and/or plasma...for each oestrous stage.

Measurement of osmolality can be measured using pooled FF, independent of oestrous stage.
Amino acids EAA
L-Arginine HCl L-Cystine L-Glutamine L-Histidine HCllH
L-Isoleucine L-Leucine L-Lysine-HCl L-Methionine L-Phenylalanine L-Threonine L-Tryptophan L-Tyrosine L-Valine L-Glutamine NEAA
L-Alanine L-AsparagineHO
L-Aspartic acid L-Glutamic acid Glycine L-Proline L-Serine Minimal Essential Medium (MEM) essential amino acid solution (no.
320-1130; Gibco).
b MEM nonessential amino acid solution (no. 320-1140; Gibco).

Specifically (stage by stage) Amino acids affected by oestrous stage included:
glutamate, asparagine, histidine, glutamine, threonine, arginine, taurine, alanine, tyrosine, tryptophan, methionine, valine, phenylalanine, leucine and lysine. The Stage I FF amino acid profile was very similar to that of plasma, particularly as regards aspartate, glutamate, serine, glycine, threonine, arginine, taurine, phenylaianine and isoleucine. By contrast, Stages ll-IV profiles were significantly lower than plasma for aspartate, glutamate, asparagine (Stage III only), serine, taurine (except Stage II), alanine (Stage II only), methionine (except Stage II), valine (except Stage II), leucine (Stage III only) and lysine (except Stage III).
Overall, plasma levels were markedly higher than those of FF for glutamate, serine and glutamine and, to a lesser extent, for asparagine, threonine, taurine, tyrosine, methionine, phenylalanine and lysine. By contrast, FF valine concentration was significantly higher than in plasma.

It is well believed that amino acids are uptaken into the oocytes by the gap junctions (gap junctional communication (GJC)).
Addtionally the GSH (glutathione) content of an oocyte is much dependent nextdoor cumulus cells, GJC is believed to contribute to GSH content in the oocyte Via passage/uptake of GSH or its substrates, cysteine and glutamine from the cumulus cells to an oocyte. The way cumulus cells regulate MPN (male pronuclei) formation is not understood.
The basic medium used for the maturation of oocytes was BSA-free Whitten's medium NaCI, KCI, KH2PO 4, MgSO 4, NaHCO 3, glucose, sodium lactate, sodium pyruvate, hemicalcium lactate, potassium penicillin, streptomycin sulfate, phenol red and supplemented with PFF (pig/cow/human) follicular fluid, TCM-199 containing hCG, eCG, glutamine, fetal calf serum...

Also, large accumulation of cumulus cells (eg. Artificial Insemination with an external ovary produced oocyte - more broadly the cumulus are responsible for Routing and Penetration of spermatoza) affects the success of penetration of spermatozoa And the large cumulus cell mass induces acrosome reaction and assists penetration into the oocyte.
(Jason 0.5%) (DLD 0.25%) (GP HW 0.25%) We can use Proton nuclear magnetic resonance ((1)H NMR) to chemically dissect any and all life fluids, including stem cell developments (proliferation stages), regeneration, meiosis, mitosis, different cells and the functions, liver and kidney filteration cells the mechanism whereby waste enters one end and is separated and the clean blood diverted back into the system, the mechanism of lung cells (including fish gills) how the absorb oxygen and release carbon dioxide and the membrane? And how this membrane performs this function... (each and every cell type and their symbiotic relationship with each and every organ and glands...
development of ovaries to oocyte, the changing make up of Newt/Salamander's (any and all amphibians (GP)regenerating tails, starfishes, jellyfish, sea cucumber, ECM/HOX changes in response to amino acids, vitamins, ions...changes, mammals/ fishesturties (near extinct species), Focusing on follicular growth and maturation, (1)H NMR is utilized to dissect intrafollicular and circulating glycoconjugates (sugar chains and N-acetyl groups), lipoproteins (CH(3) and CH(2) groups), glucose metabolites (trimethylamines, acetate and lactate), amino acids (including the above and more specifically: (glutamine/glutamate and alanine), creatine/creatinine and polyamines). Follicular fluids is measured for oestradiol and progesterone. The intrafollicular alanine and lipoproteins (CH(3) groups) went down in the dominant follicle (the future oocyte) during growth stage, while progesterone and oestradiol showed a large increase. As detailed above gonadotrophin is used to induce ovulation;

follicular maturation is noted by lessening of glycoconjugates (sugar chains), trimethylamines and acetate, less oestradiol, also additional CH(3) groups of lipoproteins and progesterone.
Re: The Idea of Varying levels of Factors for Different Phases in Mitosis for any and all Proliferation Promoting Substances has been Previously Patented by Gerard Voon, but Below is one Example of one such Substance (Ca2+) Ca2+ ions needed for mitogenesis and increase in cytosolic Ca2+ acts as a physiological trigger in cell proliferation, the different phases of the cell cycle present different dosage needs for Ca2+. Ryanodine and NiCl2 eliminated cytosolic Ca2+ and did not prevent PDGF-induced development into the G0 into G1 phase. Furthermore progression of cells into the S phase and the further mitosis were arrested, perhaps because S phase needs Ca2+ and further proliferation promoting factors is regulated by Ca2+dosages and perhaps sharp changes and maintenance levels. G1/S are arrested when there is not enough Ca2+. Emptying of Ca2+ pools during G0/G1 and S arrest growth while plating positive affect on G2 and M
cell cycle phases.
PDGF-induced (any and all) cells' proliferation requires the activity of SERCA
pumps to uptake Ca2+ pools the entry into the S phase.

Ca2+ timing (in correlation with the mitotic phases) seems to involve Ca2+
intracellular pools drainage and alternating usage of Ca2+ membrane to uptake the exogenous Ca2+
dependent (are necessary for) on the mitotic phase. PDGF (Platelet-derived growth factor) and possibly any and all other factors (especially for proliferation) binding to receptors at the surface of cellular membrane and are intertwined with changes in cytosolic Ca2+ dosages that involves a surge the a lesser steady-state elevation, involving both the drainage of Ca2+
pools and uptake of Ca2+ from the extracellular (external to the cell).

Re: Reactive Oxygen Species (ROS), reactive nitrogen species, and calcium (Ca2+) It has been suggested that plant cells sense ROS via at least three different mechanisms: (i) unidentified receptor proteins; (ii) redox-sensitive transcription factors, such as NPR1 or Heat Shock Factors; and (iii) direct inhibition of phosphatases by ROS. There is a possiblilty of interactions of ROS, reactive nitrogen species, and calcium signalling.

All three are involved in control and regulation of biological processes, such as growth, cell cycle, programmed cell death, hormone signaling, biotic and abiotic stress responses and development...and any and all phenotypic responses.

ROS, reactive nitrogen species, and calcium (Ca2+) signalling are generated at a number of intracellular locations, including mitochondria, chloroplasts, peroxisomes, and at the extracellular side of the plasma membrane. These locations signal transduction events, such as mitogen-activated protein kinase cascades, eliciting specific cellular responses. The impacts of these ROS, reactive nitrogen species, and calcium signalling on cellular processes is mediated by both the perpetuation of their production and their amelioration by scavenging enzymes such as superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT). We need further study location, amplitude, and duration of ROS, reactive nitrogen species, and calcium Functioning like Ca2+ signaling ROS (And possibly nitrogen species) is controlled by the spatial and temporal nature of its pools and drainage.

Re: Some of the Fish we Plan to Apply the Above Spawning Methods and Techniques (From External Ovaries Factories to ROS...) Scientific name Common name Acanthopagrus latus Yellowfin sea bream A. berda Picnic sea bream A. schlegeli Black sea bream A. sivicolus Southern black sea bream Anthias disper Red fish Boleophthalmus pectinirostris Pond or mud skipper Chanos chanos Milkfish Choerodon schoenleinii Black spot tusk fish Cromileptes altivelis Highfin grouper Eleutheronema tetradactylum Four finger threadfin Epinephelus akaara Red grouper E. amblycephalus* White-spotted green grouper E. awoora Yellow grouper E. coioides / suillus Red-spotted grouper E. fario Black-saddled grouper E. fuscoguttatus Tiger grouper E. lanceolatus Giant or King grouper E. malabaricus / salmonides Malabar grouper E. quoyaqnus Long-finned grouper E. tauvina Green grouper E. tukula Potato grouper E. trimaculatus Brown marbled grouper Evynnis cardinalis Golden-skinned porgy Girella melanichthys Smallscale blackfish Girella puncta* Largescale blackfish Glossogobius giuris Flathead goby Gnathanodon speciosus Kingfish Hapalogenys nitens Beard grunt Kyphosus lembus Shortfin rudderfish Lateolabrax japonicus Japanese sea bass Lates calcarffer Asian sea bass Lethrinus nebulosus Green snapper Liza macrolepis Largescale liza Lutjanus argentimaculatus Mangrove red snapper L. erythropterus Pink snapper L. johnii John's snapper L. malabaricus Firespot snapper L. monostigma Onespot snapper L. russelli Russell's snapper L. sebae Emperor snapper L. stellatus Spotted snapper Miichthys miiuy Nibe or brown croaker Mugil cephalus Grey mullet Nibea diacanthus Speckled drum Oplegnathus punctatus Spotted knifejaw Pagrus major Red sea bream Platax orbicularis Narrow-banded batfish Plectorhynchus cinctus Three-banded grunt P. pictus Three-lipped grunt Plectropomus leopardus Coral trout Polynemus plebejus Common threadfin P. sexfilis Six threadfin Pomadasys kaakan Lined silver grunter Psettodes erumei Big-mouthed flounder Pseudosciaena crocea Large yellow croaker Rachycentron canadum Cobia or Sergeantfish Scatophagus argus Spotted scat Sciaenops ocellatus Red drum Seriola dumerilli Greater yellowtail Siganus fuscescens Dusky spinefoot S. guttaus Speckled spinefoot S. oramin Yellow-spotted spinefoot Sillago sihama Sand borer S. vermiculatus Reticulated rabbitfish Sparus sarba Silver sea bream Takifu rubripes Tiger puffer Terapon jarbua Three stripe tigerfish Trachinotus blochii Pompano Re: The Drugs Successfully Used for Aquaculture (below), we plan to test their efficacy for Any And All Life Table 1. Drugs Approved for Use in Aquatic Species*

Drug Species Indication Formalin Finfish Control of certain external protozoa and monogenetic trematodes Finfish eggs Control of fungi of the family Saprolegniaceae Penaeid shrimp Control of certain external parasites Oxytetracycline Pacific salmon Marking of skeletal tissue via medicated feed Finfish Marking of skeletal tissue via immersion Salmonids Control of ulcer disease caused by Hemophilus pisicium, furunculosis caused by Aeromonas salmonicida, bacterial hemorrhagic septicemia caused by A liquefaciens and pseudomonas disease.
Catfish Control of bacterial hemorrhagic septicemia caused by A liquefaciens and pseudomonas disease Lobster Control of gaffkemia caused by Aerococcus viridans Sulfadimethoxine/ Salmonids Control of furunculosis caused by Aeromonas ormetoprim salmonicida Catfish Control of enteric septicemia caused by Edwardsiella ictaluri Sulfamerazine~ Rainbow, brook, Control of furunculosis and brown trout Tricaine Fish, amphibians, Temporary immobilization as an aid in handling Methanesulfonate and other poikilotherms Chorionic Finfish Spawning aid Gonadotropin *Data from FDA Center for Veterinary Medicine Web site.7 ~Not currently marketed.

Re: Sites and Some Hormones that Achieve our Desire to Mature Sperm and Eggs and Induce Spawning Induction of spawning by the injection of 8,11,14-eicosatrienoic acid into the coelomic cavity, shows that the natural sperm maturation factor and spawning hormone.
Alternately females, injected with female prostomial homogenate into the coelomic cavity induces oocyte maturation and subsequent spawning. Oocytes, on the other hand, although maturing in the presence of CMF (Coelomic Maturation Factor), do not mature in the presence of prostomial homogenate.
Oocyte maturation and subsequent spawning is under the control of 2 hormones:
(1) a substance from the prostomium, Prostomal Maturation Hormone (PMH), which induces the production of (2) CMF which acts on the oocyte. PMH is required for oocyte maturation...

Re: Other Chemicals to be Used on any and all Life for Sexual Maturation/Proliferation and/or Breeding/Spawning (On Both Males and Females) Tetracaine, procaine, propranolol, oxprenolol, lanthanum chloride, ionophore A23187, DL-dithiothreitol, arachidonic acid and ammonium ions (ammonium sulphate) were also all tested for their ability to induce maturation... Also possibly injection with 5-hydroxytryptamine (5-HT) into the gonads... serotonin (and any and all psychiatric drugs including viagra and/or cialis) can be applied to induce spawning/breeding... (We could possibly use Antho-RFamide, a neuropeptide situated in ciliated neurons within follicle epithelia, also causes exfoliation of the follicle epithelium from spawned follicles). Photoperiod/intensity augments the potency of Antho-RFamide. The actin-binding toxin phalloidin substantially reduced the incidence of Antho-Rfamide.

Re: Other Alternate Serotonin Drugs that may help Induce Spawning Serotonin levels increase with of tryptophan. Increasing intake foods high in tryptophan, do affect sretonin levels due to competition with other amino acids. Stamina and aerobic exercise improves mood, by increasing in serotonin (endorphins?) levels. Endorphins (endogenous opioid polypeptide compounds). Produced by the pituitary gland and the hypothalamus in vertebrates during strenuous exercise, excitement, and orgasm; and they resemble the opiates in their abilities to produce analgesia and a sense of well-being. Endorphins work as "natural fever relievers", whose effects may be enhanced by other medications. We could increase water flow rate and velocity and or exercise/activity that is enjoyable to the life form then extract the pituitary and/or hypothalamus from the exercising life form (eg.
pigs/cows/gold fish creatures that are easy, fast and large volume...) also the sources of endorphins, GnRH, Pituitary and Hypothalumus... extracts from the glands of pigs/cows/gold fish could be prepared for extraction via Gerard Voon's patented Neural Links (12% GP) to put the subject of extract in a serotonin/endorphin inducing mood... and inject to make other life (aquaculture and/or any and all life stock farming - even as a viagra and/or feel good supplement) mate... In fact we could use Neural Links directly on the life forms to induce them spawn/breed/mate/have sex (11%)...

It is believed that a diet rich in whole grain carbohydrates and low in protein will increase serotonin by secreting insulin, which helps in amino acid competition. The danger is that increasing insulin for a long period of time can lead to insulin resistance, which are factors for some causes of obesity, type 2 diabetes, and even lower serotonin levels.
Furthermore that muscles use many amino acids but not tryptophan, allowing men to have more serotonin absorption than women.

Re: Drugs that treat the Serotonin/5-HT system include - AntiDepressants .cndot. Ambilify (Aripiprazole) .cndot. Sertraline (Zoloft) .cndot. Escitalopram (Lexapro) .cndot. Fluoxetine (Prozac) .cndot. Bupropion (Welibutrin, Zyban) .cndot. Paroxetine (Paxil) .cndot. Venlafaxine (Effexor) .cndot. Trazodone (Desyrel) .cndot. Amitriptyline (Elavil) .cndot. Citalopram (Celexa) .cndot. Duloxetine (Cymbalta) .cndot. Mirtazapine (Remeron) .cndot. Nortriptyline (Pamelor) .cndot. Imipramine (Tofranil) Also (concentrated extracts of) hypericum perforatum (St John's Wort) First Generation Antipsychotics Butyrophenones .cndot. Haloperidol (Haldol) Phenothiazines .cndot. Chlorpromazine (Thorazine) .cndot. Fluphenazine (Prolixin) - Available in decanoate (long-acting) form .cndot. Perphenazine (Trilafon) .cndot. Prochiorperazine (Compazine) .cndot. Thioridazine (Mellaril) .cndot. Trifluoperazine (Stelazine) .cndot. Mesoridazine .cndot. Promazine .cndot. Triflupromazine (Vesprin) .cndot. Levomepromazine (Nozinan) .cndot. Promethazine (Phenergan) Thioxanthenes .cndot. Chlorprothixene .cndot. Flupenthixol (Depixol and Fluanxol) .cndot. Thiothixene (Navane) .cndot. Zuclopenthixol (Clopixol & Acuphase) Second generation antipsychotics .cndot. Clozapine (Clozaril) - treats schizophrenia, acute manic episodes, and maintenance of bipolar disorder.
.cndot. Risperidone (Risperdal) - treats Tourette Syndrome or Anxiety Disorder.
.cndot. Quetiapine (Seroquel) - Used primarily to treat bipolar disorder and schizophrenia, and "off-label" to treat chronic insomnia and restless legs syndrome; it is a powerful sedative .cndot. Ziprasidone (Geodon) - Now (2006) approved to treat bipolar disorder.
Dosing 20 mg twice daily initially up to 80 mg twice daily. Prolonged QT interval a concern; watch closely with patients that have heart disease; when used with other drugs that prolong QT interval potentially life-threatening.
.cndot. Amisuipride (Solian) - Selective dopamine antagonist. Higher doses (greater than 400 mg) act upon post-synaptic dopamine receptors resulting in a reduction in the positive symptoms of schizophrenia, such as psychosis. Lower doses, however, act upon dopamine autoreceptors, resulting in increased dopamine transmission, improving the negative symptoms of schizophrenia. Lower doses of amisulpride have also been shown to have anti-depressant and anxiolytic effects in non-schizophrenic patients, leading to its use in dysthymia and social anxiety disorder.
.cndot. Asenapine is a 5-HT2A- and D2-receptor antagonist under development for the treatment of schizophrenia and acute mania associated with bipolar disorder.
.cndot. Paliperidone (Invega) - Derivative of risperidone.
Third generation antipsychotics .cndot. Aripiprazole (Abilify) - reduce susceptibility to metabolic symptoms seen in some other atypical antipsychotics.[4) .cndot. Dopamine partial agonists:
.cndot. Under clinical development - Bifeprunox; nordozapine (ACP-104).
Other options .cndot. Tetrabenazine (Nitoman in Canada and Xenazine in New Zealand and some parts of Europe) is similar in function to antipsychotic drugs, though is not, in general, considered an antipsychotic itself. This is likely due to its main usefulness being the treatment of hyperkinetic movement disorders such as Huntington's Disease and Tourette syndrome, rather than for conditions such as schizophrenia. Also, rather than having the potential to cause tardive dyskinesia, which most antipsychotics have, tetrabenazine can actually be an effective treatment for the condition.
.cndot. Cannabidiol One of the main psychoactive components of cannabis. A
recent study has shown cannabidiol to be as effective as atypical antipsychotics in treating schizophrenia.

Metabotropic glutamate receptor 2 agonism has been seen as a promissing strategy in the development of novel antipsychotics. The active metabolite of this prodrug targets the brain glutamate receptors mGluR2/3 rather than dopamine receptors.

Re: Natural Humane Sources of Serotonin Serotonin are in mushrooms and plants, including fruits and vegetables.
Particularily high contents are present in nuts of the walnut (Juglans) and hickory (Carya) genuses. Serotonin are been present in plantain, pineapple, banana, kiwifruit, plums, and tomatoes. Moderate contents are present in a wide range of tested vegetables. It should be noted that serotonin, unlike its precursors 5-HTP and tryptophan, does not cross the blood-brain barrier. There are plants containing serotonin together with a family of related tryptamines that are methylated at the amino (NH2) and hydroxy (OH) groups, are N-oxides, or miss the OH group eg.
Anadenanthera genus that are used in the hallucinogen.

Re: Treatment For Anxiety that may help Spawning/Breeding/Mating/Sex and also Time of Stress for eg. Aquaculture including Transport and/or Disease and/or Majoring Cleaning of the Tank, Any time the Creatures Become Spooked:
.cndot. Imidazopyridines ~ zolpidem (Ambien) ~ alpidem ~ saripidem ~ necopidem .cndot. Pyrazolopyrimidines ~ zaleplon (Sonata) ~ indiplon ~ ocinapion .cndot. Cyclopyrrolones ~ eszopicione (Lunesta) ~ zopiclone (Imovane) ~ pagocione ~ suricione ~ pazinacione ~ suproclone .cndot. other structural families ~ Etifoxine ~ Panadiplon ~ CL-218,872 ~ SX-3228 ~ L-838,417 ~ RWJ-51204 ~ Y-23684 Growth hormone (GH) also has been identified as an oocyte survival factor acting secondarily to the IGF-I pathway. Gonadotropins or growth hormones may act to promote follicle survival, in part, by altering follicular steroidogenesis (eg. human preovulatory follicles cultured in the presence of IGF-I produced more estrogen and less progesterone.

Studies conducted on preovulatory follicles suggest that, without supportive hormones such as human chorionic gonadotropin (hCG), insulin-like growth factor-I (IGF-1), and human follicle stimulating hormone (hFSH), cultured follicle cells will undergo apoptosis (previous studies have shown). Growth hormone (GH) also has been identified as an oocyte survival factor acting secondarily to the IGF-I pathway (previous studies). In some cases, there is evidence that the various gonadotropins or growth hormones may act to promote follicle survival, in part, by altering follicular steroidogenesis. For example, human preovulatory follicles cultured in the presence of IGF-I produced more estrogen and less progesterone (shown by previous studies).
In agreement with my belief that timing/concentration (leading to - response with sexual stimulation; intensity - due the levels of (various environmental cues) change in one direction and durations versus the cue's levels of change opposite in the other direction to create cycles (any and all lengths; seasonal; annual), that the fish (any and all life), naturally take in their sexual response via genetics and/or entrainment to these environmental cues (including maturation (annual cycles and the duration and rate and levels of environmental cues - some hormonal factors such hCG, IGF-I, hFSH, GnRH) - melatonin inhibitor) and seasonal-hormonal responses impact on the willingness of fish and any and all life to spawn/mate/breed/have sex) in response to environmental cues that cause late-cycle high of plasma E2 and testosterone highs may be the cause in feedback to the brain (hypothalamus, and pituitary glands), inducing synthesis and secretion of LH at the expense of FSH production. It is believed that (estradiol-17) E2 is produced in the granulosa cells by the action of the aromatase enzyme and converts testosterone to E2.
Circulating E2 and testosterone levels rise early in final maturation, while the oocyte germinal vesicle is still migrating; peak testosterone levels respond less behind peak E2 levels.
Androgen and estrogen levels both fall coincident with the peak in circulating E2 that is concurrent with GVBD (shown by previous studies) artificial induction can be accomplished using synthetic analogue of gonadotropinreleasing hormone (GnRHa). Noted that changes in circulating steroid hormones rise of internal LH in the blood plasma is induced by GnRHa (past studies).
These observations further suggest that rises in androgen and estrogen levels...
Fish have shown cyclic pattern of oogenesis and endocrine correlative cyclic pattern.
Responding to such environmental cues (eg. photothermal - diurnal and seasonal changes in water temperature and day length) and therefore reproducing in timing cycle that is entrained. Deposition of yolk granules in the growing oocytes is triggered by decreasing day length and water temperature (eg. monsoons and/or rainy season and other environmental factors caused by such seasons and the cues that belong to such seasonal changes that the fish type and entraining that they respond to). Furthermore these seasonal related cues come with a distinct rise in concentration of estradiol-17 - ' -,(E2) and testosterone, as well as Vg.
Exogenous E2 may induce hepatic vitellogenesis suggesting its role as hormone controlling this process.

Oocyte growth begins at deposition of lipid droplets (made of wax esters, which are made from circulating triglyceride precursors) /yolk protein and yolk granules in the ooplasm. Past studies have shown that oocyte growth is correlated to yolk protein deposition in the yolk granule.

Ovarian follicles and vitellogenesis:

1. Theca is the outermost layer derived from stromal connective tissue and is made up of collagen fibers, fibroblasts, steroidogenic cells, and capillaries or other vascular components (according to past studies).
2. Germinal epithelium-derived follicle cells then flatten to form a singular granulosa cell layer under the basal lamina and on top of the cellular chorion or ZR.
3. Junctional complexes between the follicle cells allow for the extracellular passage of Vg, through the theca, basement membrane, granulosa cell layer, and ultimately to the oocyte surface.
4. The ZR then takes on a perforated form by a process known as microvillar, which emanate from both the oocyte and granulosa cells (also based on past studies).

Re: Krill (Zoo Plankton) Farming In addition to any and all oily fish sued to produce healthy fish oils...
especially we are mass producing krill and shrimp...

For their omega-3 fats, eicosapentanoic acid (EPA) and docosahexanoic acid (DHA), but hooked together in a different form...

Fish oil is extracted from, omega-3 fatty acids are found in the triglyceride form, while krill/shrimp oil are hooked up in a double chain phospholipid structure.

The EPA leg phospholipid has a molecule known as astaxanthin, which is a very potent anti-oxidant. The phospholipid EPA and DHA in krill oil are more absorbable. Also Krill/shrimp oil enters more easily into the mitochondria and the nucleus membrane. Furthermore EPA and DHA from krill oil's phospholipid have more complex profile such as phosphatidylcholine, whose effect is reductive-stress-reducing choline, and also a natural emulsifier.

Krill oil has vitamin E, vitamin A, vitamin D and canthaxanthin, like astaxanthin. The anti-oxidant potency of krill oil is 48 times more potent than fish oil. Aparently it has 10 times more antioxidant activity than beta-carotene and as much as 1,000 times more than vitamin E.
Interestingly it is a source of alpha-tocopherol (vitamin E) and a derivative, called marine-derived tocopherol, that has antioxidant properties than alpha-tocopherol. It is postulated that tocopherols enable krill survive in the cold waters. As well they have the flavonoid luteolin. If this theory is correct it may be the only non-plant version of the substance... One claim of omega-3 of krill oil is that the phospholipid content of krill oil plays a role in omega-3 passing through the blood-brain barrier.

If we can mass produce the krill/shrimp cheaply enough they are a rich fertilizer, like fish fertilizer...

Astaxanthin is used as a feed supplement for salmon, crabs, shrimp, chickens and egg production. Regardless of the source, astaxanthin provides some important benefits beyond coloration. It also has been found to be essential for proper growth and survival.

Vitamin A
Krill Oil Phospholipids, Omega-3 rich Eicosapentaenoic Acid (EPA) Docosahexaenoic Acid (DHA) Omega-6 Fatty Acids Astaxanthin Transfat Malacostraca include:

crabs, krill, pill bugs, shrimp, and relatives Kingdom: Animalia Phylum: Arthropoda Class: Malacostraca Members of this Class UV before and/or after freezing, thaw in water prior to injection and/or freeze dry.

A number of studies have shown that krill oil is very effective in: reducing LDL-cholesterol, raising HDL-cholesterol therefore cardiovascular disease; Lowering blood sugar threfore diabetes; Treating the pain; Inflammation from rheumatoid arthritis (therefore see list of inflamatory diseases pages 23 to 25) and Aches and pains (therefore as an pain releiver eg.
substitite for tylenol/aspirin...), PMS and dysmenorrhea, adult ADHD; Healthy brain and nervous system neurodegenerative diseases and It also crosses the blood-brain barrier, which makes it available to the eye, brain and central nervous system to alleviate oxidative stress that contributes to ocular, and neurodegenerative diseases such as glaucoma and Alzheimer's;
Healthy liver function; Improved immune function; Concentration and memory;
Healthy joints.
Astaxanthin is potent antioxidant, it may be beneficial in cardiovascular, immune, inflammatory and neurodegenerative diseases. Research supports the assumption that it protects body tissues from oxidative damage.

Cod Liver versus Fish Oils are: More efficiently and more easily absorbed;
Superior antioxidant protection; Can be renewable; Does not oxidize.

Re: Any and All Shrimp especially Krill Maturation and Spawning We plan to use all the aquaculture (fish, crayfish/crawdads - arthropods, amphbians, any and all life) environmental (eg. page 1 point 6. and Re: Growth and Maturation and Re; Spawning) including temperature/PH/DH and any and all environmental conditions shock, filtration of seawater and/or (eg. simulate annual seasons - including monsoon season); most important is photoperiod (melatonin might be used to alternate use during winter and non-use during the longer lighting summer) length of daylight and (also variation in intensity and/or light pulses) relative to the length of darkness), proper stimulation olfactory organs, FEO
(food entrained oscillator... proper stimulation of taste buds, food availability; water temperature; water flow rate (activity - possible impacts on metabolism )(velocity m/sec - flooding and water currents);
pH/DH (water hardness); Dissolved Oxygen (mg/I); Free carbon-dioxide (mg/I);
Total alkalinity (mg/I); Chlorides (mg/I); TDS (mg/l); DOM (mg/I); Specific conductivity (mhos)/25C, some believe in the cycles of the moon, atmospheric pressure, availability and desired quality types based on the fish type of spawning substrate, availability of aquatic plants, sticks, gravel, and safe nooks and crannies, nutrition, and the presence of other fish...The effects of these external factors on the blood concentrations of substances (eg. exercise and activity and changes in PH/DH ... and/or melatonin antagonists) could be used together with GnRH, gonadotrophin, vitamin A (beta carotene), retinoic acid, for maturing the gametes which is absolutely necessary for spawning/mating/sexual intercourse/breeding to result in healthy embryos (mitosis/meiosis)...

We plan to mass produce the krill using the above breeding/spawning techniques.

One spawning method is shrimp eyestalk ablation.

We could try to grow (regenerate) the bioreactors (proliferate via ECM and/or HOX), we could even slice the krill to a survivable cellular colonies (maybe with a threshing machine with very small and fine and sharp blades) and regrow them via regeneration ECM/HOX and other technologies Gerard Voon has patented, whereby the cells are constantly renewed as an altenative artificial maturation and spawning techniques covered in this patent. (Jeff 30 of 35%) Krill can be harvested and processed for their protein (40% or more of dry weight) and lipids (about 20% in E. superba). Their exoskeleton for chitin, hydrolytic enzymes such as proteases, carbohydrases, nucleases and phospholipases, found in their digestive gland in the cephalothorax of the krill; these enzymes might be usable in producing biomass, bio fuel and bio crops ethanol...

Other uses include krill pastes or processed krill as food additives, e.g. in the form of krill oil gel capsules.

Krill are used as pastes, for food addictives oil gel in capsules enzymes, necrotic tissue and chemonucleolytic agents.

Their oils can be used as biofuel...
Algae Algae belong to a group of micro organisms known as protists. Crypthecodinium cohnil and Schizochytrium are two strains/varieties of algae currently employed for supplements of omega-3. They are proliferated in fermenters under specific environmental conditions. When ready the algae are dried and oil separated. By creating a closed system oil from the algae is contaminants free and monoculture.

Fish oil or krill oil, both have EPA and DHA, algae has the DHA. The organisms that feed on the algae retroconvert some DHA back to EPA, although more direct EPA is often needed...
However, research suggests that DHA may be the more critical of the two omega-3s for certain health benefits, especially for infants.

DHA from algae was more positive in its effects on the growth of colon cancer cells in mice than EPA, 90 percent less tumor growth. Reducing platelet aggregation or lowering blood cholesterol as combinations of EPA and DHA from fish oils. Depression may also be associated with reduced blood levels of omega-3 fatty acids and or Omega-6...
Phytoplankton Krill probably react (spawn/sexually triggered/mate/breed in response regulated to the environmental sensitivities in height and duration to the developing cycle of) the Pacific upwelling of cool waters in spring that supports the (especially sensitive to availability - eg.
feeding food frenzy of clouds of phytoplankton) and nutritional and/or odour and/or taste and/or energy associated with phytoplankton bloom. Therefore the timing (entrained, genetically versus glandular recall) bloom is linked with the hatching of krill eggs.

Re: Micro organisms Including Diatoms And Phytoplankton Have the Following Benefits to the Life that Feed on It (Shrimp and/or Krill above Especially feed on Phytoplanktoon).
.cndot. immune system enhancement .cndot. general nutrition - provide ultra-potent lipids to enhance brain function .cndot. energy - increases energy and vitality .cndot. promotes better sleep - more restful and restorative .cndot. antioxidant protection from cancers and degenerative diseases .cndot. cardiovascular health - supports a healthy heart .cndot. blood pressure control .cndot. cholesterol reduction .cndot. liver health - supports a healthy liver .cndot. neurological support - mental alertness, ADHD, Parkinsons, and general dementia .cndot. alkalizing - the balancing of body pH away from unhealthy excess acidicity .cndot. anti-inflammatory effects on membranes - promotes relief from joint pain .cndot. cell wall improvement through increased permeability and flexibility .cndot. detoxification and cleansing - supports removal of toxins from cells and organs .cndot. skin care - such as acne, psoriasis, dermatitis .cndot. better vision - more effective than Lutein .cndot. blood sugars - stabilizes blood sugar levels (aids those who are diabetic or hyperglycemic) .cndot. supports weight loss Re: Factors that Diatoms/Phytoplankton/Plankyton/Zooplankyton/Micro Organisms that are Needed for them to Thrive Most needed seems to be iron ... others include temperature, salanity, transmissivity (suspended particulates), oxygen, nitrate, phosphate, silicate, C02 partial pressure, PH, iron binding ligands...All plants benefit from active nitrate reductase, and the ability for nitrogen fixation makes them good to fertilize hydroponic waters...

The most proliferate are the heterotrophic bacteria, ciliates, and flagellates... We are studying their genes 1. to determine the genes responsible for their rapid proliferation, to insert these genes into cow/pig/chicken ... via ovary/testes precusor and gamete cells to mass produce their meat, and/or we could insert these genes responsible for rapid proliferating into micro organisms that have strong enzymes (eg. from termites) for ethanol production, perhaps cutting the processing time and making the process easier. On the other hand we could insert DNA
and/or plasmid into and either pre genetically altered such as enucleated to be compatible with human DNA/plasmid (so these micro organisms: heterotrophic bacteria, ciliates, and flagellates) can reproduce with eg. human DNA, for future extarction into stem cells seed DNA
and/or any other uses for any and all life DNA... These procedures with these micro organisms, can also be used with nitrogen fixing with healthy fertility increasing microorganisms in soil, compost and/or mushrooms, any and all life (especially cells) that we want to mass produce rapidly (harnesssing the rapid rate of proliferation of these heterotrophic bacteria, ciliates, and flagellates)...

Diatoms Include .cndot. Diatom A cont. C cont.

A .cndot. Attheya longicornis .cndot. Chaetocerotaceae .cndot. Attheya septentrionalis .cndot. Clepsydra (genus) .cndot. Asterionella .cndot. Auxospore .cndot. Coscinodiscineae .cndot. Attheya .cndot. Attheya arenicola B D
.cndot. Attheya armata .cndot. Attheya decora .cndot. Bacteriastrum .cndot. Didymosphenia geminata .cndot. Attheya flexuosa .cndot. Biddulphiineae .cndot. Attheya gaussii F
C
.cndot. Frustule .cndot. Centrales .cndot. Chaetoceros p .cndot. Chaetoceros diadema .cndot. Chaetoceros furcellatus .cndot. Pennales .cndot. Phaeodactylum tricornutum .cndot. Pinnularia T

.cndot. Thalassiosira pseudonana We can collect diatoms resting spores shallower floors/beds of water...scooping the resting spores versus trying to reach them in deeper waters. We will grow them including krill by putting silicon, nitrogen, phosphorus and iron and adjusting temperatures - we could grow them with hay fusion, filamentous algae, cladophora, vaucheria... also dphania and brine shrimp. When it comes time for the Krill to spawn we control the the conditional factors including providing an abundance of food and we move them to a deeper pen...

Re: Easy to Grow Sources of Protein Supplements 1. Proteins extracted from Earth Worms and/or grubs - freeze dried/powdered.
2. Proteins extracted from ants, crushed/pulverized (their exoskeleton perhaps dissolved in acid and/or enzymes as long as the method of removing the exoskeleton - if need be at all does devalue the protein desired) frozen and/or freeze dried.
3. These ants and eartheworms can be fed sugar and/or restaurant waste (shredded).

4. They can all be treated to UV then (eg. flash frozen) frozen and then UV
again then thawed and fed as protein.
5. We could add algae taste and/or smell to make the above sources of food more appetizing (pellets).

Since these sources of protein are so cheap, as an alternate for human supplements/additive (pills/capsules/powder), for animal feed and/or any and all fish feed.
And or pwdered and fed to animal meat in bioreactors to mass produce mass amounts of meat cheaply, or as fertilizer and also to gro fat cells (from eg. seals, whales and walruses) to produce biofuel and also a delicacy (GP 0.5%).

Re: Micro organisms Lacto Bacillius and/or Actinomycetes, diatoms... both of which are photosynthetic bacterias -which can be used for pre digestion of feed for any and all creatures - making the food more nutritional. Lacto Bacillus also produces lactic acid from sugars, carbohydrates and yeast (which sterilizes - we could produce antibacterial hand washes/antibacterial wet tissues/gels... lactic acid could also possibly mixed with chitin and/or furanone - anti agent used against harmful organisms), lactic acid also ferments and decomposes lignin and cellulose, which can be used for any and all biocrops to turn into ethanol.

Re: Biofilms/Quorum Sensing - believed to be sensed via pheromone and/or autoinducers by secreting signal molecules; cell to cell signaling We could possibly use genes that govern high virulence (eg. E.Coli)and genetically Modify into cows/pigs/chickens/fish meats such that we can clone fast growing life forms but also the meats for food. Also we could use biofilm/quorum sensing for yeast, bacteria in cow' s mouth (that produces methane - for fuel), micro organisms found in a cow's/horses'/
gut for cellulose (eg. hay) processing - fermentation (and their enzymes), micro organisms for compost, sewage, manure, restaurant waste, possibly garbage waste; by adding sugar we turn soils fertile...these materials can be (thoroughly/evenly/strategically) mixed with sugar/carbohydrates/starch is such a way that the clusters of waste that are easier to breakdown need less sugar, while the clusters of waste that are harder to breakdown need the supplement of higher concentration of sugar/carbohydrates/starch to give the micro organisms energy and vitality together with adequate biofilm/quorum sensing for the micro organisms to flourish.

The following (below) suggests that there are many factors affecting micro organism in the blood stream or occupying infected cells, actually some similarity to fish (any and all life) and their responses to contents (and concentration) and conditions in the water (medium)... We are using melatonin antagonist for micro organisms that are light sensitive, we also will control any and all environment factors (the same as in fish maturation) in cycles (tall rise and fall of and timing that cause cycle and durational changes), as well as the hypothalamus and pituitary gland hormones (and all their analogues) as well as LH...We could also try viagra, cialis, LHRHa (with dopamine agonist), Beta Carotene, (Vitamin A), retinoic acid, water melon rind, Kapok (Ceiba pentandra) bark.. .

Re: Regulation of virulence (Cited Prof. Dr. Hubert Hilbi, SS04) - Most of which is well known and widely available in other Studies .cndot. DNA structure and transcription factors .cndot. Environment - temperature, pH, ions, osmolarity, oxygen, nutrients .cndot. Host cells - contact - intracellular environment .cndot. Cell density (quorum sensing) - autoinducer systems - processes (bioluminescence, biofilm, virulence) .cndot. Genome structure and topology .cndot. Environment - temperature, growth phase, pH, ions, osmolarity, oxygen, nutrients .cndot. Host cells - secretion-dependent regulation - intracellular environment (macrophages, amoebae) .cndot. Cell density (quorum sensing) - autoinducer systems - processes (bioluminescence, virulence, biofilm) Ceiba pentandra bark is believed to be diuretic, aphrodisiac, and to treat headache, as well as type II diabetes. We could test the effectiveness and isolate the active ingredient then commercially make the active ingredient(s) available.

Re: Photoperiod and the Natural Production and Injested Vitamin D

Tissues in the body convert Vitamin (via receptors and enzymes), from Vitamin D; 25-hydroxyvitamin D and/or 25(OH) into active ; 1,25-dihydroxyvitamin D.

Vitamin D interacts with 200 genes.

We are particularily interested in smooth muscle growth (as a perhaps cyclical response in coordination with the seasonal light and dark photoperiods) - thus the level, timing of change, steadyness (not too volatile versus times when is needs to be volatile to induce the eg. fish (any and all life)), of rate of change the duration it prolongs the high and lows in mimicing or even find a cyclical combination that works even better for sexual maturation and/or spawning/breeding/sex/mating ... if the postulation in the paragraph below is correct than it might help any and all tissue not only including the testes and the ovaries but all life and their body parts, including stem cells in Gerard Voon's bioreactor... Perhaps the ability of Vitamin D
to help those with dysfunctioning Seasonal Affective Disorder (SAD) may help growth, sexual maturation and sex/mating/breeding/spawning...

An interesting postulation is that Vitamin D may be released naturally into the organism during Photoperiod (response to sun light), and therefore may play an opposite role than melatonin (released in darkness). Thus Vitamin D may play a role in sexual maturity and growth...

We testing the effectiveness of Vitamin D on any and all (anti inflammatory) of the below diseases:

Cardiovascular Blood Pressure Multiple Sclerosis (MS) Rheumatoid Arthritis (RA) Inflammatory Bowel Disease (IBD) Interstitial Cystitis (IC) Fibromyalgia (FM) Autonomic nervous dysfunction (AND neural-mediated hypotension);
Pyoderma Gangrenosum (PG) Chronic Fatigue (CF) and Chronic Fatigue Syndrome (CFS).
Chronic hepatitis Systemic lupus erythematosus Arthritis Thyroidosis Scieroderma Diabetes mellitus Graves' disease Beschet's disease and Graft versus host disease (graft rejection).
Chronic inflammatory pathologies such as aneurysms Hemorrhoids Sarcoidosis Chronic inflammatory bowel disease Ulcerative colitis Crohn's disease and vascular inflammatory pathologies Disseminated intravascular coagulation Atherosclerosis Kawasaki's pathology Coronary artery disease Hypertension Stroke Asthma Chronic hepatitis Multiple sclerosis Peripheral neuropathy Chronic or recurrent sore throat Laryngitis Tracheobronchitis Chronic vascular headaches (including migraines Cluster headaches and tension headaches) and pneumonia Neurodegenerative diseases including Demyelinating diseasessuch as multiple sclerosis and acute transverse myelitis;
Extrapyramidal and cerebellar disorders such as lesions of the corticospinal system;
Disorders of the basal ganglia or cerebellar disorders;
Hyperkinetic movement disorders such as Huntington's Chorea and senile chorea;

Drug-induced movement disorders such as those induced by drugs which block CNS

dopamine receptors;
Hypokinetic movement disorders such as Parkinson's disease;
Progressive supranucleo palsy;
Cerebellar and Spinocerebellar Disorders such as astructural lesions of the cerebellum;
Spinocerebellar degenerations (spinal ataxia) Friedreich's ataxia Cerebellar cortical degenerations Multiple systems degenerations (MencelDejerine-Thomas Shi-Drager and Machado Joseph)); and systemic disorders (Refsum's disease Abetalipoprotemia, ataxia telangiectasia and mitochondrial multi-system disorder);
Demyelinating core disorders such as:
Multiple sclerosis Acute transverse myelitis;
Disorders of the motor unit such as neurogenic muscular atrophies (anterior horn cell degeneration) such as Amyotrophic lateral sclerosis Infantile spinal muscular atrophy and juvenile spinal muscular atrophy);
Alzheimer's disease;
Down's Syndrome in middle age;
Diffuse Lewy body disease; Senile Dementia of Lewy body type;
Wernicke-Korsakoff syndrome;
Chronic alcoholism;
Creutzfeldt-Jakob disease;
Subacute sclerosing panencephalitis Hallerrorden-Spatz disease; and Dementia pugilistica Malignant pathologies involving tumors or other malignancies such as:
Leukemias (acute chronic myelocytic chronic lymphocytic and/or myelodyspastic syndrome);
Lymphomas (Hodgkin's and non-Hodgkin's lymphomas such as malignant lymphomas (Burkitt's lymphoma or Mycosis fungoides));
Carcinomas (such as colon carcinoma) and metastases thereof;
Cancer-related angiogenesis;
Infantile hemangiomas;
Alcohol-induced hepatitis.
Ocular neovascularization Psoriasis Duodenal ulcers Other diseases that we are testing for effectiveness include parkinsons, diabetes, osteoporosis, multiple sclerosis, breast cancer, colon cancer, prostate cancer, autoimmune disease, cardiovascular (blood clotting - posssibly in gel, powder or imbedded in gauze (perhaps with antibacterials such as furanone and/or chitin) to an open wound to prevent bleeding to death and then apply a layer of ECM/HOX for regeneration assuming that the wound is not too severe - in which case it may be better to grow an entire new limb using the ECM scaffolding and stem cells of a recently deceased body part donor disease), any and all sexually transmitted diseases, herpes...hepatitus, skin problems eg. serious acne, any and all skin conditions, depression, schizophrenia, seasonal affective disorder, peripheral artery disease, tuberculosis, cancer, periodontal disease, multiple sclerosis,...

Using all our Gerard Voon's patented inventions (Methods and Techniques, Models) Titled:
BioScience and all the above: Fish liver oils, including cod liver oil, Fatty fish species, Herring, Catfish, Salmon, cooked, Mackerel, Sardines, canned in oil, Tuna, canned in oil, Eel, One whole egg (perhaps more duck eggs because of their oiliness).

EPA healthy heart - healthy body Promotes a healthy heart and circulatory system Supports healthy homocysteine levels Supports proper immune function Promotes good mood and emotional well-being Supports joint flexibility DHA healthy mood - healthy mind Essential for memory, cognitive function, learning and focus Supports a healthy pregnancy Promotes good mood and emotional well-being Reduces harmful effects of stress Supports the visual and brain development of fetus' and infants GIA healthy skin & hair - healthy hormones Promotes beneficial prostaglandins that maintain hormonal balance Provides a "feel good" effect, improves mood Nourishes hair and skin Supports joint flexibility Promotes normal body fat metabolism Vitamin D Receptor will be used to help us in cell proliferation and/or differentiation (from stem cells to any and all life as well as micro organisms). We will also use its properties via Gerard Voon's patented stem cells to immune cells (adjuvant and immune cells priming as a serum for diseses with antigens or unchanging intracellular proteins - for harmful diseases that mutate their cellular antigens on their membranes; or just fortify the immune response during disease out break), Vitamin D is also involved in white blood cells eg. monocytes and activated T and B
cells.

Re: Waterbourne mirco organisms (eg. diatoms) could be added with sugar (in its various forms)...white rice...to Hydronics...and any and all mediums.

The waterbourne micro organisms (eg. diatoms), can break down ammonia/nitrates/urea/sugars/carbohydrates/sewage/detritus/hay fusion/compost/tea manure.. in the hydroponic media...to make the water fertile for plants and algae...
Re: Drawing 1. - Tall Greenhouse Solar and Mirror Invention

1. Solar Panels on the roof of the Greenhouse.

2. Mirrors on the bottom ceiling to catch reflected light from angled mirrors surrounding th greenhouse.

3. The greenhouse plants space.

4. The mirrors angled to aim sun's light at the mirrors in the ceiling (possibly parabolic).
The reason the greenhouse is so tall is to allow surrounding mirrors further out to also aim and reach the ceiling...

Re: Cure for Baldness and Fur Industry We take a newly deceased donor, we take off the scalp (and follicular skin below) hair and/or fur and all and treat its raw side with ECM/HOX (Jeff 20% of 35%) any and all proliferating factors and/or flush the skin/flesh under the hair/fur with deterregent and until only the ECM

sccafold remains add stem cells from the own patient (to avoid rejection) (to grow a whole new scalp with hair/fur or in the case of fur, we could cut off the top skin for fur/skin...(also aligators/crocodiles and/or eels...) while leaving the bottom layer of follicle base behind that we expose ECM/HOX (Jeff 10% of 35%) and/or any and all proliferation factors to regenerate the fur for human bald patients we then operate on the patient to either descalp and/or dermabrasion the patient's upper layer of then attach the new scalp to the bald patient.

(Jeff 2.5%)