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CA2643744A1 - Cancer imaging and treatment - Google Patents

Cancer imaging and treatment Download PDF

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Publication number
CA2643744A1
CA2643744A1 CA002643744A CA2643744A CA2643744A1 CA 2643744 A1 CA2643744 A1 CA 2643744A1 CA 002643744 A CA002643744 A CA 002643744A CA 2643744 A CA2643744 A CA 2643744A CA 2643744 A1 CA2643744 A1 CA 2643744A1
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Prior art keywords
arg
tyr
orn
nal
gly
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CA002643744A
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French (fr)
Inventor
Hans Jurgen Wester
Norman Koglin
Markus Schwaiger
Horst Kessler
Burkhardt Laufer
Oliver Demmer
Martina Anton
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Technische Universitaet Muenchen
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Priority claimed from GB0603901A external-priority patent/GB0603901D0/en
Priority claimed from GB0610962A external-priority patent/GB0610962D0/en
Application filed by Individual filed Critical Individual
Publication of CA2643744A1 publication Critical patent/CA2643744A1/en
Abandoned legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K7/00Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/64Cyclic peptides containing only normal peptide links
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/04Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
    • A61K38/12Cyclic peptides, e.g. bacitracins; Polymyxins; Gramicidins S, C; Tyrocidins A, B or C
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/02Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
    • A61K51/04Organic compounds
    • A61K51/08Peptides, e.g. proteins, carriers being peptides, polyamino acids, proteins
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K51/00Preparations containing radioactive substances for use in therapy or testing in vivo
    • A61K51/02Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
    • A61K51/04Organic compounds
    • A61K51/08Peptides, e.g. proteins, carriers being peptides, polyamino acids, proteins
    • A61K51/088Peptides, e.g. proteins, carriers being peptides, polyamino acids, proteins conjugates with carriers being peptides, polyamino acids or proteins
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents
    • A61P35/04Antineoplastic agents specific for metastasis
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574Immunoassay; Biospecific binding assay; Materials therefor for cancer

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Epidemiology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Optics & Photonics (AREA)
  • Genetics & Genomics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oncology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Hospice & Palliative Care (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

A compound, or a pharmaceutically acceptable salt or ester thereof, comprises a ligand for the chemokine receptor CXCR4 and a detectable label, the ligand having a binding affinity for the CXCR4 receptor, measured as IC50 in the presence of 125I-CPCR4, of 25OnM or lower, wherein the ligand comprises a cyclic oligopeptide moiety having the motif B-Arg or B- (Me)Arg within the cyclic moiety, and wherein B is a basic amino acid; a derivative thereof, or phenylalanine, provided that the motif is B-Arg when B is a N.alpha.-methyl derivative of a basic amino acid. In preferred embodiments, the cyclic oligopeptide moiety has the sequence: cyclo[D-Tyr/(Me)D-Tyr - B - Arg/(Me)Arg - Z - (Ala)n - X] wherein: B is as defined above; Z is an amino acid containing an aromatic group in its side chain; n is 1 or 0, provided that n is 1 only when the preceding four amino acids in the cyclic moiety sequence are D-Tyr/(Me)D-Tyr-Arg-Arg-Nal, NaI being L-3-(2-naphthyl)alanine; and X is selected from GIy, (Me)GIy, Ala, Dap (diaminopropionic acid), Dap(FP) ((N- fluoropropionyl)-diaminopropionic acid), Dab (diaminobutyiic acid), Dab(FP) ((N- fluoropropionyl)-diaminobutyric acid), Dab(FB) ((N-fluorobenzoyl)-diaminobutyric acid) and Dap(FB) ((N-fluorobenzoyl)-diaminopiOpionic acid). The compounds may be useful for diagnostic imaging and/or therapeutic purposes.

Claims (54)

1. A compound, or a pharmaceutically acceptable salt or ester thereof, comprising a ligand for the chemokine receptor CXCR4 and a detectable label, the ligand having a binding affinity for the CXCR4 receptor, measured as IC50 in the presence of CPCR4, of 250nM or lower, wherein the ligand comprises a cyclic oligopeptide moiety having the motif B-Arg or B-(Me)Arg within the cyclic moiety, and wherein B
is a basic amino acid, a derivative thereof, or phenylalanine, provided that the motif is B-Arg when B is a N .alpha.-methyl derivative of a basic amino acid.
2. A compound according to claim 1, wherein the cyclic oligopeptide moiety has the sequence:

cyclo[D-Tyr/(Me)D-Tyr - B - Arg/(Me)Arg - Z -(Ala)n - X]
wherein:

B is as defined in claim 1;

Z is an amino acid containing an aromatic group in its side chain;

n is 1 or 0, provided that n is 1 only when the preceding four amino acids in the cyclic moiety sequence are D-Tyr/(Me)D-Tyr-Arg-Arg-Nal, Nal being L-3-(2-naphthyl)alanine; and X is selected from Gly, (Me)Gly, Ala, Dap (diaminopropionic acid), Dap(FP) ((N-fluoropropionyl)-diaminopropionic acid), Dab (diaminobutyric acid), Dab(FP) ((N-fluoropropionyl)-diaminobutyric acid), Dab(FB) ((N-fluorobenzoyl)-diaminobutyric acid) and Dap(FB) ((N-fluorobenzoyl)-diaminopropionic acid).
3. A compound according to claim 2 wherein Z is selected from Nal, Dap(FB) or AMS(FB) (an oxime of aminooxy serine and 4-fluorobenzaldehyde).
4. A compound according to any of claims 1 to 3 wherein B is selected from Arg, Orn, D-Orn, Cit and His, or N-substituted derivatives thereof.
5. A compound according to any preceding claim wherein B is N .alpha.-substituted with a Me group.
6. A compound according to any of claims 2 to 5 wherein B is Orn or D-Orn, the ornithine residue being substituted at N .delta. with one or two groups selected from fluolobenzoyl (FB), fluoropropionyl (FP), acetyl (Ac), amido (Am), Me, 1-naphthylmethyl (N1), 2-naphthylmethyl (N2), benzyl (Bz) and acyl spacer moieties, wherein the acyl spacer moiety is an acyl group containing a chain of 1-14 carbons, optionally interrupted by heteroatoms, and having a nucleophilic functional group at its end distal to the ornithine N .delta..
7. A compound according to claim 6 wherein the acyl spacer moiety is selected from aminohexanoyl (Ahx), triethyleneglycolamino acyl (TGAS), (Ahx)2, (Ahx)3, (TGAS)2 and (TGAS)3.
8. A compound according to claim 6 wherein B is D-Orn substituted at N .alpha.
with a Me group.
9. A compound according to claim 6 wherein B is Orn substituted at N .delta.
with FB, FP, Ac, Am, N1, N2, Me and N1, Me and N2, Bz, Bz and FB, Bz and FP, Me and FB, Me and FP, or Me.
10. A compound according to claim 6 wherein B is D-Orn substituted at N
.delta. with FB, FP, Me and FB, or Me and FP, and optionally substituted at N .alpha. with a Me group.
11. A compound according to any of claim 1 to 5, wherein the cyclic oligopeptide moiety has the sequence: cyclo[D-Tyr - B - Arg - Z - X], wherein B, Z and X are as defined in claim 2, provided that not more than one of the residues in the said sequence may be N .alpha.-methylated.
12. A compound according to claim 11, wherein B is Arg.
13. A compound according to any of claim 1 to 4, wherein the cyclic oligopeptide moiety has the sequence: cyclo[D-Tyr/(Me)D-Tyr - B - Arg/(Me)Arg - Z - X], wherein Z
and X are as defined in claim 2 and wherein B is selected from Arg, (Me)Arg, Orn, Cit, Orn(FB), Orn(FP), Orn(Ac), Orn(Am), Orn(N1), Orn(N2), Orn(Me, N1), Orn(Me, N2), Orn(Me), Orn(Bz), Orn(Bz,FB), Orn(Ahx), Orn(Ahx2), Orn(Ahx3), Orn(TGAS), Orn(TGAS2), Orn(TGAS3), Orn(Me,FB), D-Orn(FB), (Me)D-Orn(FB), (Me)D-Orn(Me,FB), His and Phe, provided that not more than one of the residues in the said sequence may be N.alpha.-methylated.
14. A compound according to claim 13, wherein the first residue is D-Tyr.
15. A compound according to claim 13 or claim 14, wherein the third residue is Arg.
16. A compound according to any of claims 11 to 14, wherein Z is Nal.
17. A compound according to any of claims 11 to 15, wherein X is Gly.
18. A compound according to any of claims 1 to 4, wherein the cyclic oligopeptide moiety has a sequence selected from cvclo[D-Tyr - Arg - Arg - Nal - Gly]
cyclo[D-Tyr - (Me)Arg - Arg - Nal - Gly]
cyclo[D-Tyr - Arg - (Me)Arg - Nal - Gly]
cyclo[D-Tyr - Arg - Arg - Nal - (Me)Gly]
cyclo[D-Tyr - Orn - Arg - Nal - Gly]
cyclo[D-Tyr - Cit - Arg - Nal - Gly]
cyclo[D-Tyr - Arg - Arg - Nal -Ala - Gly]
cyclo[D-Tyr - Arg - Arg - Nal -Ala - Ala]
cyclo[D-Tyr - (Me)Arg - Arg - Nal - (Me)Gly]
cyclo[D-Tyr - (Me)Arg - Arg - (Me)Nal -Gly]
cyclo[(Me)D-Tyr - Arg - Arg - Nal -Ala - Gly]
cyclo[(Me)D-Tyr - Arg - Arg - Nal - Gly]
cyclo[D-Tyr - Orn(FB)- Arg - Nal - Gly]
cyclo[D-Tyr - Orn(FP) - Arg - Nal - Gly]
cyclo[D-Tyr - Orn(Ac) - Arg - Nal - Gly]
cyclo[D-Tyr - Orn(Am) - Arg - Nal - Gly]
cyclo[D-Tyr - Arg - Arg - Nal - Dap(FP)]
cyclo[D-Tyr - Orn(N1) - Arg - Nal - Gly]
cyclo[D-Tyr - Orn(N2) - Arg - Nal - Gly]
cyclo[D-Tyr - Orn(Me,N1) - Arg - Nal - Gly]
cyclo[D-Tyr - Orn(Me,N2) - Arg - Nal - Gly]
cyclo[D-Tyr - Orn(Me) - Arg - Nal - Gly]
cyclo[D-Tyr - Orn(Bz) - Arg - Nal - Gly]
cyclo[D-Tyr - Orn(Bz,FB) - Arg - Nal - Gly]
cyclo[D-Tyr - Orn(Ahx) - Arg - Nal - Gly]
cyclo[D-Tyr - Orn(Ahx3) - Arg - Nal - Gly]
cyclo[D-Tyr - Orn(TGAS) - Arg - Nal - Gly]
cyclo[D-Tyr - Orn(TGAS2) - Arg - Nal - Gly]
cyclo[D-Tyr - Orn(TGAS3) - Arg - Nal - Gly]

cyclo[D-Tyr - Orn(Me,FB)- Arg - Nal - Gly]
cyclo[D-Tyr - D-Orn(FB) - Arg - Nal - Gly]
cyclo[D-Tyr - (Me)D-Orn(FB) - Arg - Nal - Gly]
cyclo[D-Tyr - (Me)D-Orn(Me,FB) - Arg - Nal - Gly]
cyclo[D-Tyr - His - Arg - Nal - Gly]
cyclo[D-Tyr - Phe - Arg - Nal - Gly]
19. A compound according to claim 18 wherein the cyclic oligopeptide moiety has a sequence selected from cyclo[D-Tyr - Arg - Arg - Nal - Gly]
cyclo[D-Tyr - (Me)Arg - Arg - Nal - Gly]
cyclo[D-Tyr - Arg - (Me)Arg - Nal - Gly]
cyclo[D-Tyr - Orn - Arg - Nal - Gly]
cyclo[D-Tyr - Cit - Arg - Nal - Gly]
cyclo[D-Tyr - Orn(FB)- Arg - Nal - Gly]
cyclo[D-Tyr - Orn(FP) - Arg - Nal - Gly]
cyclo[D-Tyr - Orn(Ac) - Arg - Nal - Gly]
cyclo[D-Tyr - Orn(Arn) - Arg -Nal - Gly]
cyclo[D-Tyr - Orn(N1) - Arg - Nal - Gly]
cyclo[D-Tyr - Orn(N2) - Arg - Nal - Gly]
cyclo[D-Tyr - Orn(Me,N1) - Arg - Nal - Gly]
cyclo[D-Tyr - Orn(Me,N2) - Arg - Nal - Gly]
cyclo[D-Tyr - (Me)D-Orn(FB) - Arg - Nal - Gly]
cyclo[D-Tyr - His - Arg - Nal - Gly]
20. A compound according to claim 19 wherein the cyclic oligopeptide moiety has a sequence selected from cyclo[D-Tyr - Orn - Arg - Nal - Gly]
cyclo[D-Tyr - Orn(FB)- Arg - Nal - Gly]
cyclo[D-Tyr - (Me)D-Orn(FB) - Arg - Nal - Gly]
21. A compound according to any preceding claim, wherein the ligand consists of the cyclic oligopeptide moiety.
22. A compound according to any preceding claim, wherein the label is a radiolabel.
23. A compound according to claim 22, wherein the compound contains one or more Dap(FB), Dap(FP), FB or FP groups and one of the fluorine substituents is 18F.
24. A compound according to claim 23 wherein the 18F is present on an FB or FP

substituent at N.delta. of Orn or D-Orn.
25. A compound according to claim 22, having a radiolabel selected from 18F, 47Sc, 51Cr, 52Fe, 52Mm, 56Ni, 57Ni, 62Cu, 64Cu, 67Ga, 68Ga, 72As, 75Br, 76Br, 77Br, 82 Br, 89Zr, 94m Tc, 97Ru, 99m Tc, 111In, 123I, 124I, 125I, 131I, 191Pt, 197Hg, 201T1, 203Pb, 110m In, 120I.
26. A compound according to claim 22 having a radiolabel which is attached to the ligand by means of a complex between an organic complexation agent and a radionuclide, the complex being attached to the ligand in such a way as not to destroy its binding properties to the CXCR4 receptor.
27. A compound according to claim 26, wherein the complexation agent is attached to the ligand by means of a spacer group.
28. A compound according to claim 22, wherein the radiolabel is selected from 32P, 67Cu, 77As, 90Y, 99Mo, 103Ru, 105Ph, 109Pd, 111Ag, 114m In, 117m Sn, 121Sn, 127Te, 131I, 140La, 140Nd, 142Pr, 143Pr, 149Tb, 149Pm, 151Pm, 153Sm, 159Gd, 161Tb, 166Ho, 166Dy, 169Er, 169Yb, 172Tm,175Yb, 177Lu, 186Re, 188Re, 198Au, 199Au, 211At, 211Bi, 212Bi, 213Bi , 225Ac.
29. A compound according to claim 22, wherein the radiolabel is selected from 90Y, 188Re and 1311.
30. A compound, or a pharmaceutically acceptable salt or ester thereof, comprising a cytotoxic moiety and a ligand for the chemokine receptor CXCR4, the ligand having a binding affinity for the CXCR4 receptor, measured as IC50 in the presence of CPCR4, of 250nM or lower, wherein the ligand comprises a cyclic oligopeptide moiety having the motif B-Arg or B-(Me)Arg within the cyclic moiety, and wherein B
is a basic amino acid, a derivative thereof, or phenylalanine, provided that the motif is B-Arg when B is a N.alpha.-methyl derivative of a basic amino acid.
31. A compound according to claim 30, wherein the cyclic oligopeptide moiety has the sequence:

cyclo[D-Tyr/(Me)D-Tyr - B - Arg/(Me)Arg - Z-(Ala)n - X]
wherein:

B is a basic amino acid, a derivative thereof, or phenylalanine, provided that the motif is B-Arg when B is a N.alpha.-methyl derivative of a basic amino acid, or a pharmaceutically acceptable salt or ester thereof, Z is an amino acid containing an aromatic group in its side chain;

n is I or 0, provided that n is 1 only when the preceding four amino acids in the cyclic moiety sequence are D-Tyr/(Me)D-Tyr-Arg-Arg-Nal; and X is selected from Gly, (Me)Gly, Ala, Dap (diaminopropionic acid), Dap(FP) ((N-fluoropropionyl)-diaminopropionic acid), Dab (diaminobutyric acid), Dab(FP) ((N-fluoropropionyl)-diaminobutyric acid), Dab(FB) ((N-fluorobenzoyl)-diaminobutyric acid) and Dap(FB) ((N-fluorobenzoyl)-diaminopropionic acid).
32. A compound according to claim 31 wherein Z is selected from Nal (L-3-(2-naphthyl)alanine), Dap(FB) or AMS(FB).
33. A compound, or a pharmaceutically acceptable salt or ester thereof, comprising a ligand for the chemokine receptor CXCR4, the ligand having a binding affinity for the CXCR4 receptor, measured as IC50 in the presence of 125I-CPCR4, of 250nM or lower, wherein the ligand comprises a cyclic oligopeptide moiety having the motif B-Arg or B-(Me)Arg within the cyclic moiety, and wherein B is a basic amino acid, a derivative thereof, or phenylalanine, provided that the motif is B-Arg when B
is a N.alpha.-methyl derivative of a basic amino acid, and provided that the oligopeptide moiety does not have the sequence cyclo[D-Tyr - Arg - Arg - Nal - Gly], nor the sequence cyclo[D-Tyr - Orn - Arg - Nal - Gly].
34. A compound according to claim 33, the ligand comprising a cyclic oligopeptide moiety having the sequence:

cyclo[D-Tyr/(Me)D-Tyr - B - Arg/(Me)Arg - Z - (Ala)n - X]
wherein:

B is as defined in claim 33;

Z is an amino acid containing an aromatic group in its side chain;

n is 1 or 0, provided that n is 1 only when the preceding four amino acids in the cyclic moiety sequence are D-Tyr/(Me)D-Tyr-Arg-Arg-Nal; and X is selected from Gly, (Me)Gly, Ala, Dap (diaminopropionic acid), Dap(FP) ((N-fluoropropionyl)-diaminopropionic acid), Dab (diaminobutyric acid), Dab(FP) ((N-fluoropropionyl)-diaminobutyric acid), Dab(FB) ((N-fluorobenzoyl)-diaminobutyric acid) and Dap(FB) ((N-fluorobenzoyl)-diaminopropionic acid).
35. A compound according to claim 34 wherein Z is selected from Nal (L-3-(2-naphthyl)alanine), Dap(FB) or AMS(FB).
36. A compound according to any preceding claim which has been modified by the attachment of one or more hydrophilic moieties.
37. A pharmaceutical composition comprising a compound according to any preceding claim, together with one or more pharmaceutically acceptable excipients.
38. A composition according to claim 37 suitable for injection.
39. A method of synthesis of a compound according to claim 1, the method comprising treating the ligand with a source of a radionuclide under conditions such that the radionuclide, or a complex between an organic complexation agent and the radionuclide, becomes attached to the ligand.
40. A compound according to any of claims 1 to 36, for use in therapy or diaposis.
41. Use of a compound according to any of claims 1 to 36 in the preparation of a medicament for the treatment of a neoplastic condition.
42. Use of a compound according to any of claims 1 to 29 in the preparation of a medicament for the diagnostic imaging of a neoplastic condition.
43. Use according to claim 41 or claim 42 wherein the neoplasia has, or is suspected of having, metastatic potential.
44. Use according to any of claims 41 to 43 wherein the neoplastic condition is breast or prostate cancer.
45. A method of imaging neoplastic tissue, the method comprising the administration, to a subject having or suspected of having a neoplasia, of a compound according to any of claims 1 to 29, and the detection of the compound following distribution thereof in vivo.
46. A method according to claim 45 including the further step, following the detection step, of generating an image of the detected compound.
47. A method of determining the metastatic potential of cells of a neoplasia, the method comprising exposing the cells to a compound according to any of claims 1 to 29, so as to allow the compound to bind to CXCR4 receptors on the surface of the cells, removing unbound compound from the vicinity of the cells, and determining the presence and/or amount of compound bound to the cells.
48. A method according to claim 47 wherein the cells are removed from the neoplasia and exposed to the compound in vitro.
49. A method according to any of claims 45 to 47 wherein the imaging, or determination of the presence and/or amount of bound compound, is performed using PET or SPECT
when the label comprises a radionuclide.
50 50. A method of treatment of a neoplastic condition in a subject, the neoplasia having, or being suspected of having, metastatic potential, the method comprising the administration to the subject of a compound according to any of claims 1 to 36.
51. A method according to any of claims 45 to 50 wherein the neoplastic condition is breast or prostate cancer.
52. A method of synthesis of a compound according to any of claims 1 to 29, the method comprising treating the ligand with a source of the detectable label under conditions such that the detectable label, or a complex between an organic complexation agent and the label, becomes bound to the ligand.
53. A method of synthesis of a compound according any of claims 30 to 32, the method comprising treating the ligand with a source of the cytotoxic moiety under conditions such that the cytotoxic moiety becomes bound, directly or indirectly, to the ligand.
54. A compound, or a pharmaceutically acceptable salt or ester thereof, comprising a ligand for the chemokine receptor CXCR4, wherein the ligand comprises a cyclic oligopeptide moiety having the sequence:

cyclo[D-Tyr/(Me)D-Tyr - B - Arg/(Me)Arg - Z-(Ala)n - X]
wherein:

B is as defined in claim 1;

Z is an amino acid containing an aromatic group in its side chain;
n is 1 or 0; and X is selected from Gly, (Me)Gly, Ala, Dap (diaminopropionic acid), Dap(FP) ((N-fluoropropionyl)-diaminopropionic acid), Dab (diaminobutyric acid), Dab(FP) ((N-fluoropropionyl)-diaminobutyric acid), Dab(FB) ((N-fluorobenzoyl)-diaminobutyric acid) and Dap(FB) ((N-fluorobenzoyl)-diaminopropionic acid), the compound optionally comprising a detectable label, provided that, when the compound does not comprise a detectable label, the cyclic oligopeptide moiety does not have the sequence cyclo[D-Tyr - Arg - Arg - Nal - Gly], nor the sequence cyclo[D-Tyr - Orn - Arg - Nal -Gly].
CA002643744A 2006-02-27 2007-02-27 Cancer imaging and treatment Abandoned CA2643744A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB0603901.0 2006-02-27
GB0603901A GB0603901D0 (en) 2006-02-27 2006-02-27 Cancer imaging and treatment
GB0610962A GB0610962D0 (en) 2006-06-02 2006-06-02 Cancer imaging and treatment
GB0610962.3 2006-06-02
PCT/GB2007/000684 WO2007096662A2 (en) 2006-02-27 2007-02-27 Cancer imaging and treatment

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US (2) US8628750B2 (en)
EP (1) EP1991562A2 (en)
JP (2) JP5308829B2 (en)
KR (1) KR20090041360A (en)
CA (1) CA2643744A1 (en)
WO (1) WO2007096662A2 (en)

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Publication number Priority date Publication date Assignee Title
CA2643744A1 (en) * 2006-02-27 2007-08-30 Technische Universitaet Muenchen Cancer imaging and treatment
JO2776B1 (en) 2007-05-30 2014-03-15 ايلي ليلي اند كومباني Cyclic Peptide CXCR4 Antagonists
GB0716897D0 (en) 2007-08-30 2007-10-10 Univ Muenchen Tech Cancer imaging and treatment
WO2010053572A2 (en) 2008-11-07 2010-05-14 Massachusetts Institute Of Technology Aminoalcohol lipidoids and uses thereof
EP2380596A1 (en) * 2010-04-20 2011-10-26 Technische Universität München Cyclopentapeptide derivatives and uses thereof
EP2380597A1 (en) * 2010-04-20 2011-10-26 Technische Universität München Cyclopeptide derivatives and uses thereof
JP6145404B2 (en) * 2010-05-07 2017-06-14 エフ・ホフマン−ラ・ロシュ・アクチェンゲゼルシャフト Diagnostic methods for ex vivo cell detection
KR101306157B1 (en) * 2010-09-10 2013-09-10 연세대학교 산학협력단 Compositions for Preventing or Treating Tumors Comprising Cyclopentapeptides
CA2831392C (en) 2011-03-28 2020-04-28 Massachusetts Institute Of Technology Conjugated lipomers and uses thereof
PE20181541A1 (en) 2011-10-27 2018-09-26 Massachusetts Inst Technology DERIVATIVES OF AMINO ACIDS FUNCTIONALIZED IN THE N TERMINAL, CAPABLE OF FORMING DRUG ENCAPSULATING MICROSPHERES
ES2647076T3 (en) * 2012-06-06 2017-12-19 Polyphor Ag Beta fork peptidomimetics
WO2014028487A1 (en) 2012-08-13 2014-02-20 Massachusetts Institute Of Technology Amine-containing lipidoids and uses thereof
HRP20191254T1 (en) 2014-05-21 2019-10-18 Entrada Therapeutics, Inc. PEPTIDES INPETING CELLS AND PROCEDURES FOR THEIR PRODUCTION AND USE
US10815276B2 (en) 2014-05-21 2020-10-27 Entrada Therapeutics, Inc. Cell penetrating peptides and methods of making and using thereof
EP3587409B8 (en) 2014-05-30 2022-07-13 Translate Bio, Inc. Biodegradable lipids for delivery of nucleic acids
CN106573959B (en) 2014-06-06 2022-03-25 慕尼黑工业大学 Modified cyclic pentapeptides and uses thereof
KR102511554B1 (en) 2014-06-24 2023-03-16 샤이어 휴먼 지네틱 테라피즈 인크. Stereochemically enriched compositions for delivery of nucleic acids
WO2016004202A1 (en) 2014-07-02 2016-01-07 Massachusetts Institute Of Technology Polyamine-fatty acid derived lipidoids and uses thereof
EP3185880B1 (en) 2014-08-27 2020-02-12 Ohio State Innovation Foundation Improved peptidyl calcineurin inhibitors
LT3310764T (en) 2015-06-19 2023-06-12 Massachusetts Institute Of Technology Alkenyl substituted 2,5-piperazinediones and their use in compositions for delivering an agent to a subject or cell
US9890187B2 (en) 2015-06-26 2018-02-13 Epos-Iasis Research And Development, Ltd. Prototype systems of theranostic biomarkers for in vivo molecular management of cancer
EP3509612A4 (en) * 2016-09-06 2021-07-21 Mainline Biosciences Cxcr4 antagonists and methods of use
WO2019050564A1 (en) * 2017-09-05 2019-03-14 Mainline Biosciences High affinity cxcr4 selective binding conjugate and method for using the same
US10639379B2 (en) * 2017-09-05 2020-05-05 Mainline Biosciences Llc High affinity CXCR4 selective binding conjugate and method for using the same
US11123437B2 (en) 2017-09-05 2021-09-21 Mainline Biosciences, Inc. Selective CXCR4 binding peptide conjugate and methods for making and using the same
WO2019070962A1 (en) 2017-10-04 2019-04-11 Ohio State Innovation Foundation Bicyclic peptidyl inhibitors
WO2019148194A2 (en) 2018-01-29 2019-08-01 Ohio State Innovation Foundation Peptidyl inhibitors of calcineurin-nfat interaction
US11793884B2 (en) 2018-01-29 2023-10-24 Ohio State Innovation Foundation Cyclic peptidyl inhibitors of CAL-PDZ binding domain
WO2019165183A1 (en) 2018-02-22 2019-08-29 Entrada Therapeutics, Inc. Compositions and methods for treating mitochondrial neurogastrointestinal encephalopathy
EP3790890A4 (en) 2018-05-09 2022-03-02 Ohio State Innovation Foundation CYCLIC, CELL-PENETRATING PEPTIDES WITH ONE OR MORE HYDROPHOBIC RAILS
WO2020053255A1 (en) 2018-09-12 2020-03-19 Technische Universität München Therapeutic and diagnostic agents for cancer
CA3108450A1 (en) 2018-09-12 2020-03-19 Technische Universitat Munchen Cxcr4-targeted diagnostic and therapeutic agents with reduced species selectivity
WO2021150258A1 (en) * 2020-01-26 2021-07-29 Mainline Biosciences Llc Isotopically labelled selective cxcr4 binding peptide conjugate and methods for making and using the same
EP4043041A1 (en) 2021-02-15 2022-08-17 Technische Universität München Cxcr4-ligands for diagnostic and therapeutic use and precursors thereof
WO2024259228A1 (en) * 2023-06-16 2024-12-19 Prime Medicine, Inc. Cyclic peptide lipids for nanoparticle formulations

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4444260A1 (en) * 1994-12-13 1996-06-20 Hoechst Ag Cyclohexapeptides and their mixtures, process for their preparation and their use
WO1999040947A2 (en) * 1998-02-11 1999-08-19 Resolution Pharmaceuticals Inc. Angiogenesis targeting molecules
PL350998A1 (en) 1999-03-24 2003-02-24 Anormed Inc Chemokine recpetor binding heterocyclic compounds
US6319675B1 (en) 1999-11-24 2001-11-20 Millennium Pharmaceuticals, Inc. Methods for detecting and/or identifying agents which bind and/or modulate function of “bonzo” chemokine receptor
ES2747357T3 (en) 2001-03-14 2020-03-10 Dako Denmark As MHC molecule constructs and their uses for diagnosis and therapy
WO2003066830A2 (en) 2002-02-08 2003-08-14 Genetastix Corporation Human monoclonal antibodies against membrane proteins
US20040009149A1 (en) * 2002-02-27 2004-01-15 Altman John D. Multimeric binding complexes
AU2003261723A1 (en) 2002-08-27 2004-03-19 Takeda Chemical Industries, Ltd. Cxcr4 antagonist and use thereof
JP4391123B2 (en) * 2002-10-24 2009-12-24 株式会社オーファンリンク Novel CXCR4 antagonist
JP2006524242A (en) 2003-03-27 2006-10-26 エモリー ユニバーシティー CXCR4 antagonists and methods of their use
EP1620567A1 (en) 2003-04-15 2006-02-01 Hans-Jürgen Thiesen Method for diagnosing rheumatoid arthritis or osteoarthritis
WO2004094465A2 (en) 2003-04-23 2004-11-04 The Board Of Trustees Of The University Of Illinois Office Of Technology Management University Of Illinois At Urbana-Champaign Synthetic molecules that mimic chemokines
NO20035681D0 (en) * 2003-12-18 2003-12-18 Amersham Health As Optical imaging of lung cancer
CA2643744A1 (en) * 2006-02-27 2007-08-30 Technische Universitaet Muenchen Cancer imaging and treatment
GB0716897D0 (en) * 2007-08-30 2007-10-10 Univ Muenchen Tech Cancer imaging and treatment

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