CA2598602A1 - Quantification par image de la translocation moleculaire - Google Patents
Quantification par image de la translocation moleculaire Download PDFInfo
- Publication number
- CA2598602A1 CA2598602A1 CA002598602A CA2598602A CA2598602A1 CA 2598602 A1 CA2598602 A1 CA 2598602A1 CA 002598602 A CA002598602 A CA 002598602A CA 2598602 A CA2598602 A CA 2598602A CA 2598602 A1 CA2598602 A1 CA 2598602A1
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- Prior art keywords
- cell
- cells
- nuclear
- molecular
- compartment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US55348404P | 2004-03-16 | 2004-03-16 | |
| US60/553,484 | 2004-03-16 | ||
| US57287704P | 2004-05-19 | 2004-05-19 | |
| US60/572,877 | 2004-05-19 | ||
| PCT/US2005/008866 WO2005098430A2 (fr) | 2004-03-16 | 2005-03-16 | Quantification par image de la translocation moleculaire |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2598602A1 true CA2598602A1 (fr) | 2005-10-20 |
Family
ID=34972698
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002598602A Abandoned CA2598602A1 (fr) | 2004-03-16 | 2005-03-16 | Quantification par image de la translocation moleculaire |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US8150136B2 (fr) |
| EP (1) | EP1800124B1 (fr) |
| AT (1) | ATE538138T1 (fr) |
| CA (1) | CA2598602A1 (fr) |
| WO (1) | WO2005098430A2 (fr) |
Families Citing this family (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7450229B2 (en) | 1999-01-25 | 2008-11-11 | Amnis Corporation | Methods for analyzing inter-cellular phenomena |
| US8005314B2 (en) | 2005-12-09 | 2011-08-23 | Amnis Corporation | Extended depth of field imaging for high speed object analysis |
| US8131053B2 (en) * | 1999-01-25 | 2012-03-06 | Amnis Corporation | Detection of circulating tumor cells using imaging flow cytometry |
| US8885913B2 (en) | 1999-01-25 | 2014-11-11 | Amnis Corporation | Detection of circulating tumor cells using imaging flow cytometry |
| US8406498B2 (en) | 1999-01-25 | 2013-03-26 | Amnis Corporation | Blood and cell analysis using an imaging flow cytometer |
| AU2002213157A1 (en) | 2000-10-12 | 2002-04-22 | Amnis Corporation | System and method for high numeric aperture imaging systems |
| US7280207B2 (en) | 2001-07-25 | 2007-10-09 | Applera Corporation | Time-delay integration in a flow cytometry system |
| WO2005090945A1 (fr) | 2004-03-16 | 2005-09-29 | Amnis Corporation | Procede d'imagerie et d'analyse differentielle de cellules |
| US8953866B2 (en) | 2004-03-16 | 2015-02-10 | Amnis Corporation | Method for imaging and differential analysis of cells |
| ATE538138T1 (de) | 2004-03-16 | 2012-01-15 | Amnis Corp | Auf bildlicher darstellung beruhende quantifizierung molekularer translokation |
| US7996188B2 (en) | 2005-08-22 | 2011-08-09 | Accuri Cytometers, Inc. | User interface for a flow cytometer system |
| US8017402B2 (en) | 2006-03-08 | 2011-09-13 | Accuri Cytometers, Inc. | Fluidic system for a flow cytometer |
| US8303894B2 (en) | 2005-10-13 | 2012-11-06 | Accuri Cytometers, Inc. | Detection and fluidic system of a flow cytometer |
| US7780916B2 (en) | 2006-03-08 | 2010-08-24 | Accuri Cytometers, Inc. | Flow cytometer system with unclogging feature |
| US8283177B2 (en) | 2006-03-08 | 2012-10-09 | Accuri Cytometers, Inc. | Fluidic system with washing capabilities for a flow cytometer |
| US8715573B2 (en) | 2006-10-13 | 2014-05-06 | Accuri Cytometers, Inc. | Fluidic system for a flow cytometer with temporal processing |
| WO2008058217A2 (fr) | 2006-11-07 | 2008-05-15 | Accuri Instruments Inc. | Cuve de circulation pour système de cytométrie en flux |
| US7739060B2 (en) * | 2006-12-22 | 2010-06-15 | Accuri Cytometers, Inc. | Detection system and user interface for a flow cytometer system |
| US8432541B2 (en) | 2007-12-17 | 2013-04-30 | Accuri Cytometers, Inc. | Optical system for a flow cytometer with an interrogation zone |
| US8189884B2 (en) * | 2008-03-26 | 2012-05-29 | General Electric Company | Methods for assessing molecular expression of subcellular molecules |
| US8507279B2 (en) | 2009-06-02 | 2013-08-13 | Accuri Cytometers, Inc. | System and method of verification of a prepared sample for a flow cytometer |
| US8451524B2 (en) * | 2009-09-29 | 2013-05-28 | Amnis Corporation | Modifying the output of a laser to achieve a flat top in the laser's Gaussian beam intensity profile |
| US8237786B2 (en) * | 2009-12-23 | 2012-08-07 | Applied Precision, Inc. | System and method for dense-stochastic-sampling imaging |
| US8779387B2 (en) * | 2010-02-23 | 2014-07-15 | Accuri Cytometers, Inc. | Method and system for detecting fluorochromes in a flow cytometer |
| US8817115B1 (en) | 2010-05-05 | 2014-08-26 | Amnis Corporation | Spatial alignment of image data from a multichannel detector using a reference image |
| WO2011159708A1 (fr) | 2010-06-14 | 2011-12-22 | Accuri Cytometers, Inc. | Système et procédé de création d'un réseau de cytomètres de flux |
| CN103168225B (zh) | 2010-10-25 | 2015-11-25 | 阿库里赛托梅特斯公司 | 用于收集流式细胞仪中的数据集的系统和用户接口 |
| WO2014103137A1 (fr) | 2012-12-27 | 2014-07-03 | ソニー株式会社 | Système d'analyse cellulaire, programme d'analyse cellulaire et procédé d'analyse cellulaire |
| US10163203B2 (en) | 2013-11-08 | 2018-12-25 | Sony Corporation | Cell analysis system, cell analysis program and cell analysis method |
| EP3163287B1 (fr) | 2015-10-30 | 2022-03-23 | Sysmex Corporation | Procédé d'obtention d'informations de cellule et appareil d'obtention d'informations de cellule |
| JP7187474B2 (ja) | 2017-03-31 | 2022-12-12 | ライフ テクノロジーズ コーポレーション | フローサイトメトリーを撮像するための装置、システム、および方法 |
Family Cites Families (165)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3555280A (en) | 1966-04-19 | 1971-01-12 | Hycon Mfg Co | Automatic focus sensor and control |
| US3497690A (en) | 1967-09-21 | 1970-02-24 | Bausch & Lomb | Method and apparatus for classifying biological cells by measuring the size and fluorescent response thereof |
| DE1597211B1 (de) | 1967-09-26 | 1970-06-11 | Fernseh Gmbh | Farbfernsehkamera |
| JPS49133042A (fr) | 1973-04-09 | 1974-12-20 | ||
| NL7807532A (nl) | 1978-07-13 | 1980-01-15 | Akzo Nv | Metaal-immunotest. |
| US4414575A (en) | 1980-11-21 | 1983-11-08 | Hitachi Denshi Kabushiki Kaisha | Autofocus system |
| US4677680A (en) | 1982-08-31 | 1987-06-30 | Dai Nippon Insatsu Kabushiki Kaisha | Method and device for inspecting image |
| US4703017C1 (en) | 1984-02-14 | 2001-12-04 | Becton Dickinson Co | Solid phase assay with visual readout |
| JP2531605B2 (ja) | 1984-02-24 | 1996-09-04 | 株式会社東芝 | 画像の位置合せ装置 |
| US5122453A (en) | 1984-03-28 | 1992-06-16 | Technicon Instruments Corporation | Method for discriminating surface stained lymphocytes |
| JPS60258671A (ja) | 1984-06-05 | 1985-12-20 | Nec Corp | プロセツサ |
| US5096807A (en) | 1985-03-06 | 1992-03-17 | Murex Corporation | Imaging immunoassay detection system with background compensation and its use |
| US4662742A (en) | 1985-05-10 | 1987-05-05 | Becton, Dickinson And Company | Scatter/fluorescene beam splitter in a flow cytometry apparatus |
| US4845197A (en) | 1985-09-09 | 1989-07-04 | Agri-Diagnostics Associates | Monoclonal antibodies and methods for fungal pathogen detection |
| US5985549A (en) | 1985-10-22 | 1999-11-16 | University Of Massachusetts | Non-isotopic in-situ hybridization method for detection of nucleic acids |
| US4770992A (en) | 1985-11-27 | 1988-09-13 | Den Engh Gerrit J Van | Detection of specific DNA sequences by flow cytometry |
| US4777525A (en) | 1985-12-23 | 1988-10-11 | Preston Jr Kendall | Apparatus and method for a multi-resolution electro-optical imaging, display and storage/retrieval system |
| CA1338860C (fr) | 1987-04-13 | 1997-01-21 | Peter Parker | Marquage de proteines a l'aide de radio-isotopes |
| US4786165A (en) | 1986-07-10 | 1988-11-22 | Toa Medical Electronics Co., Ltd. | Flow cytometry and apparatus therefor |
| EP0281327B1 (fr) | 1987-02-27 | 1993-06-30 | EASTMAN KODAK COMPANY (a New Jersey corporation) | Réactif immunoréactif, méthode pour sa préparation et son utilisation pour déterminer une espèce immunoréactive |
| EP0280559B1 (fr) | 1987-02-27 | 1993-10-20 | EASTMAN KODAK COMPANY (a New Jersey corporation) | Essai immunologique d'agglutination et trousse de réactifs pour la détermination d'une espèce immunollogique polyvalente utilisant une solution saline de nettoyage tamponnée |
| US4857453A (en) | 1987-04-07 | 1989-08-15 | Syntex (U.S.A.) Inc. | Immunoassay device |
| EP0560410B1 (fr) | 1987-04-27 | 2002-10-02 | Inverness Medical Switzerland GmbH | Dispositif d'analyse pour l'exécution d'essais de liaisons spécifiques |
| US4959302A (en) | 1987-08-20 | 1990-09-25 | Endogen, Inc. | Method of evaluating immune system response to allografts, viral infection and immunosuppressive therapy |
| US5986061A (en) | 1988-10-28 | 1999-11-16 | Pbl Biomedical Laboratories | Phosphorylated fusion proteins |
| IL92124A (en) | 1988-10-28 | 1996-10-31 | Sidney Pestka | Recombinant proteins modified to contain post-phosphorylation that do not occur in nature |
| EP0462221A1 (fr) | 1989-03-07 | 1991-12-27 | Syngene, Inc. | Hybridation in situ en suspension pour la detection ou la separation de cellules |
| US5266486A (en) | 1989-05-12 | 1993-11-30 | Nvl Photronics Corporation | Method and apparatus for detecting biological activities in a specimen |
| US5107522A (en) | 1990-02-05 | 1992-04-21 | Sharp Kabushiki Kaisha | Automatic frequency control circuit |
| JP3181050B2 (ja) | 1990-04-20 | 2001-07-03 | 株式会社日立製作所 | 投影露光方法およびその装置 |
| US5159642A (en) | 1990-07-13 | 1992-10-27 | Toa Medical Electronics Co., Ltd. | Particle image analyzing apparatus |
| US5141609A (en) | 1990-11-16 | 1992-08-25 | The Trustees Of The Leland Stanford Junior University | Method and device employing time-delayed integration for detecting sample components after separation |
| US5257182B1 (en) | 1991-01-29 | 1996-05-07 | Neuromedical Systems Inc | Morphological classification system and method |
| US5817462A (en) | 1995-02-21 | 1998-10-06 | Applied Spectral Imaging | Method for simultaneous detection of multiple fluorophores for in situ hybridization and multicolor chromosome painting and banding |
| US5784162A (en) | 1993-08-18 | 1998-07-21 | Applied Spectral Imaging Ltd. | Spectral bio-imaging methods for biological research, medical diagnostics and therapy |
| JP3084295B2 (ja) | 1991-02-27 | 2000-09-04 | シスメックス株式会社 | フローイメージサイトメータ |
| JP3084296B2 (ja) | 1991-02-27 | 2000-09-04 | シスメックス株式会社 | フローイメージサイトメータ |
| JP3121849B2 (ja) | 1991-02-27 | 2001-01-09 | シスメックス株式会社 | フローイメージサイトメータ |
| JPH0734012B2 (ja) | 1991-02-27 | 1995-04-12 | 東亜医用電子株式会社 | フローイメージサイトメータ |
| US5436144A (en) | 1991-04-08 | 1995-07-25 | Health Research, Inc. | Process for performing PCR in mammalian cells |
| US5568315A (en) | 1991-05-28 | 1996-10-22 | Discovision Associates | Optical beamsplitter |
| US5548395A (en) | 1991-09-20 | 1996-08-20 | Toa Medical Electronics Co., Ltd. | Particle analyzer |
| JP3212647B2 (ja) | 1991-10-24 | 2001-09-25 | シスメックス株式会社 | イメージングフローサイトメータ |
| JP3102935B2 (ja) | 1991-11-20 | 2000-10-23 | シスメックス株式会社 | イメージングフローサイトメータ |
| US5686960A (en) | 1992-01-14 | 1997-11-11 | Michael Sussman | Image input device having optical deflection elements for capturing multiple sub-images |
| US5422712A (en) | 1992-04-01 | 1995-06-06 | Toa Medical Electronics Co., Ltd. | Apparatus for measuring fluorescent spectra of particles in a flow |
| JP3145486B2 (ja) | 1992-06-12 | 2001-03-12 | シスメックス株式会社 | イメージングフローサイトメータ |
| US5351311A (en) | 1992-07-28 | 1994-09-27 | The United States Of America As Represented By The Secretary Of The Navy | Neural network for detection and correction of local boundary misalignments between images |
| US6159686A (en) | 1992-09-14 | 2000-12-12 | Sri International | Up-converting reporters for biological and other assays |
| US5733721A (en) | 1992-11-20 | 1998-03-31 | The Board Of Regents Of The University Of Oklahoma | Cell analysis method using quantitative fluorescence image analysis |
| JPH08506664A (ja) | 1993-02-01 | 1996-07-16 | セック,リミテッド | Dna配列決定の方法および装置 |
| US5547849A (en) | 1993-02-17 | 1996-08-20 | Biometric Imaging, Inc. | Apparatus and method for volumetric capillary cytometry |
| US5596401A (en) | 1993-09-16 | 1997-01-21 | Toa Medical Electronics Co., Ltd. | Particle analyzing apparatus using a coherence lowering device |
| JP3290786B2 (ja) | 1993-11-26 | 2002-06-10 | シスメックス株式会社 | 粒子分析装置 |
| JP3344635B2 (ja) | 1993-12-27 | 2002-11-11 | シャープ株式会社 | カラー液晶表示装置 |
| EP0745217A4 (fr) | 1994-01-21 | 1998-07-29 | Coulter Corp | Sondes de viabilite pour isoler, identifier et/ou analyser des cellules |
| JPH07261149A (ja) | 1994-03-23 | 1995-10-13 | Seiko Instr Inc | 液晶装置 |
| JPH07286953A (ja) | 1994-04-19 | 1995-10-31 | Toa Medical Electronics Co Ltd | イメージングフローサイトメータ |
| JP3156503B2 (ja) | 1994-05-27 | 2001-04-16 | 松下電器産業株式会社 | 固体撮像装置の駆動方法及び固体撮像装置の信号処理回路 |
| US5658744A (en) | 1994-07-22 | 1997-08-19 | The United States Of America As Represented By The Department Of Health And Human Services | Methods of identifying patients having an altered immune status |
| AU3544995A (en) | 1994-09-20 | 1996-04-09 | Neopath, Inc. | Apparatus for identification and integration of multiple cell patterns |
| US5621460A (en) | 1994-10-11 | 1997-04-15 | Lockheed Martin Corporation | Optical differentiation between plants and background utilizing a single CCD camera |
| US5695934A (en) | 1994-10-13 | 1997-12-09 | Lynx Therapeutics, Inc. | Massively parallel sequencing of sorted polynucleotides |
| US5625048A (en) | 1994-11-10 | 1997-04-29 | The Regents Of The University Of California | Modified green fluorescent proteins |
| US5556790A (en) | 1994-12-05 | 1996-09-17 | Pettit; John W. | Method for Automated DNA sequencing |
| JP3481631B2 (ja) | 1995-06-07 | 2003-12-22 | ザ トラスティース オブ コロンビア ユニヴァーシティー イン ザ シティー オブ ニューヨーク | 能動型照明及びデフォーカスに起因する画像中の相対的なぼけを用いる物体の3次元形状を決定する装置及び方法 |
| US5844670A (en) | 1995-07-28 | 1998-12-01 | Ricoh Co., Ltd. | Method of and systems for measuring eccentricity of an aspherical lens surface |
| FI97665C (fi) | 1995-11-21 | 1997-01-27 | Planmed Oy | Menetelmät ja laitteet kohteen kuvantamisessa |
| US6007994A (en) | 1995-12-22 | 1999-12-28 | Yale University | Multiparametric fluorescence in situ hybridization |
| US5874304A (en) | 1996-01-18 | 1999-02-23 | University Of Florida Research Foundation, Inc. | Humanized green fluorescent protein genes and methods |
| US5764792A (en) | 1996-01-19 | 1998-06-09 | Oncor, Inc. | Method and apparatus for processing images |
| JP3640461B2 (ja) | 1996-04-03 | 2005-04-20 | シスメックス株式会社 | 粒子分析装置 |
| US5989835A (en) | 1997-02-27 | 1999-11-23 | Cellomics, Inc. | System for cell-based screening |
| US5959953A (en) | 1996-07-03 | 1999-09-28 | Zen Research Nv | Methods and apparatus for performing cross-talk correction in a multi-track optical disk reader based on magnification error |
| US5929986A (en) | 1996-08-26 | 1999-07-27 | Kaiser Optical Systems, Inc. | Synchronous spectral line imaging methods and apparatus |
| US5760899A (en) | 1996-09-04 | 1998-06-02 | Erim International, Inc. | High-sensitivity multispectral sensor |
| US5939281A (en) | 1996-09-16 | 1999-08-17 | Case Western Reserve University | Detecting alloreactivity |
| US5754291A (en) | 1996-09-19 | 1998-05-19 | Molecular Dynamics, Inc. | Micro-imaging system |
| US5855753A (en) | 1996-11-26 | 1999-01-05 | The Trustees Of Princeton University | Method for electrohydrodynamically assembling patterned colloidal structures |
| JP3683591B2 (ja) | 1997-02-27 | 2005-08-17 | セロミックス インコーポレイテッド | 細胞に基づくスクリーニングシステム |
| US6259807B1 (en) | 1997-05-14 | 2001-07-10 | Applied Imaging Corp. | Identification of objects of interest using multiple illumination schemes and finding overlap of features in corresponding multiple images |
| DE69838067T2 (de) | 1997-05-23 | 2008-03-13 | Bioarray Solutions Ltd. | Farbkodierung und in situ abfrage von matrix-gekoppelten chemischen verbindungen |
| JP4294740B2 (ja) | 1997-05-23 | 2009-07-15 | ソレクサ・インコーポレイテッド | 分析物の系列的プロセシングのためのシステムおよび装置 |
| US20040241759A1 (en) | 1997-06-16 | 2004-12-02 | Eileen Tozer | High throughput screening of libraries |
| US5926283A (en) | 1997-07-12 | 1999-07-20 | Optical Insights, Llc | Multi-spectral two dimensional imaging spectrometer |
| WO1999006941A2 (fr) | 1997-07-31 | 1999-02-11 | University Of California, Berkeley | Dispositif et procedes de traitement d'images et de signaux |
| WO2000006989A2 (fr) | 1998-07-27 | 2000-02-10 | Ljl Biosystems, Inc. | Appareil et procedes d'identification des effets d'attenuation dans des essais de mesure de luminescence |
| US5900942A (en) | 1997-09-26 | 1999-05-04 | The United States Of America As Represented By Administrator Of National Aeronautics And Space Administration | Multi spectral imaging system |
| US6115119A (en) | 1997-10-21 | 2000-09-05 | Bigelow Laboratory For Ocean Science | Device and method for studying particles in a fluid |
| AUPP032897A0 (en) | 1997-11-12 | 1997-12-04 | University Of Queensland, The | Oligomer libraries |
| US6051835A (en) | 1998-01-07 | 2000-04-18 | Bio-Rad Laboratories, Inc. | Spectral imaging apparatus and methodology |
| US6510319B2 (en) | 1998-02-05 | 2003-01-21 | Lucent Technologies Inc. | Method for optimizing forward link power levels during soft handoffs in a wireless telecommunications network |
| JP3747621B2 (ja) | 1998-03-26 | 2006-02-22 | コニカミノルタオプト株式会社 | カラー投影表示装置 |
| JPH11295208A (ja) | 1998-04-13 | 1999-10-29 | Sysmex Corp | 粒子撮像装置 |
| US20010012620A1 (en) | 1998-06-03 | 2001-08-09 | Rich Ivan N. | Method of reducing cell proliferation by inducing apoptosis in differentially selected cell subpopulations |
| US6150176A (en) | 1998-06-09 | 2000-11-21 | The Regents Of The University Of California | Fluorescent protein sensors for measuring the pH of a biological sample |
| JP4533536B2 (ja) | 1998-09-03 | 2010-09-01 | トレリス バイオサイエンス, インコーポレイテッド | 複数色の標識 |
| US6330081B1 (en) | 1998-11-20 | 2001-12-11 | Agfa Corporation | Crosstalk cancellation in a multi-color CCD signal processor |
| JP3871456B2 (ja) | 1998-12-10 | 2007-01-24 | シスメックス株式会社 | 粒子画像分析装置 |
| US6549664B1 (en) | 1998-12-31 | 2003-04-15 | Siros Technologies, Inc. | Sparse modulation codes for holographic data storage |
| US6256096B1 (en) | 1999-01-11 | 2001-07-03 | Softray | Flow cytometry apparatus and method |
| US20010006416A1 (en) | 1999-01-11 | 2001-07-05 | Johnson Paul E. | Ribbon flow cytometry apparatus and methods |
| US7450229B2 (en) | 1999-01-25 | 2008-11-11 | Amnis Corporation | Methods for analyzing inter-cellular phenomena |
| US6580504B1 (en) | 1999-01-25 | 2003-06-17 | Amnis Corporation | Multipass cavity for illumination and excitation of moving objects |
| US20060257884A1 (en) | 2004-05-20 | 2006-11-16 | Amnis Corporation | Methods for preparing and analyzing cells having chromosomal abnormalities |
| US6608682B2 (en) | 1999-01-25 | 2003-08-19 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
| US7057732B2 (en) | 1999-01-25 | 2006-06-06 | Amnis Corporation | Imaging platform for nanoparticle detection applied to SPR biomolecular interaction analysis |
| US6707551B2 (en) | 2000-01-24 | 2004-03-16 | Amnis Corporation | Multipass cavity for illumination and excitation of moving objects |
| US6473176B2 (en) | 1999-01-25 | 2002-10-29 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
| US6671044B2 (en) | 1999-01-25 | 2003-12-30 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells in broad flat flow |
| US6249341B1 (en) | 1999-01-25 | 2001-06-19 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
| US6975400B2 (en) | 1999-01-25 | 2005-12-13 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
| US6150121A (en) | 1999-02-16 | 2000-11-21 | Wisconsin Alumni Research Foundation | Assessing immunological state of transplant recipients |
| EP1151319A1 (fr) | 1999-02-19 | 2001-11-07 | Fox Chase Cancer Center | Techniques de decomposition de donnees complexes |
| US6381363B1 (en) | 1999-03-15 | 2002-04-30 | Grass Valley (U.S.), Inc. | Histogram-based segmentation of images and video via color moments |
| US6330361B1 (en) | 1999-03-16 | 2001-12-11 | Litton Systems, Inc. | Adaptively aligned optical correlator and method |
| US6156465A (en) | 1999-04-12 | 2000-12-05 | Cymbolic Sciences Inc. | Crosstalk correction |
| WO2001011341A2 (fr) * | 1999-08-05 | 2001-02-15 | Cellomics, Inc. | Systeme criblage de cellules |
| US6716588B2 (en) | 1999-12-09 | 2004-04-06 | Cellomics, Inc. | System for cell-based screening |
| EP1240498B1 (fr) | 1999-12-23 | 2008-12-17 | Illumina, Inc. | Decodage de capteurs de reseaux a microspheres |
| US6727066B2 (en) | 2000-07-28 | 2004-04-27 | Incyte Corporation | Genes expressed in treated human C3A liver cell cultures |
| US6934408B2 (en) | 2000-08-25 | 2005-08-23 | Amnis Corporation | Method and apparatus for reading reporter labeled beads |
| US6778263B2 (en) | 2000-08-25 | 2004-08-17 | Amnis Corporation | Methods of calibrating an imaging system using calibration beads |
| US6583865B2 (en) | 2000-08-25 | 2003-06-24 | Amnis Corporation | Alternative detector configuration and mode of operation of a time delay integration particle analyzer |
| US6608680B2 (en) | 2000-08-25 | 2003-08-19 | Amnis Corporation | TDI imaging system for kinetic studies |
| WO2002017219A1 (fr) | 2000-08-25 | 2002-02-28 | Amnis Corporation | Mesure de la vitesse de petits objets mobiles comme les cellules |
| US6875973B2 (en) | 2000-08-25 | 2005-04-05 | Amnis Corporation | Auto focus for a flow imaging system |
| CA2417233A1 (fr) * | 2000-08-29 | 2002-03-07 | Yeda Research & Development Co. Ltd. | Methodes d'isolation de genes codant des proteines a fonction specifique et de criblage d'agents actifs d'un point de vue pharmaceutique |
| WO2002026195A2 (fr) | 2000-09-29 | 2002-04-04 | Clinomics Biosciences, Inc. | Micro-reseaux de tissus tumoraux |
| US6563583B2 (en) | 2000-10-12 | 2003-05-13 | Amnis Corporation | Multipass cavity for illumination and excitation of moving objects |
| CA2426798C (fr) * | 2000-10-27 | 2012-02-21 | Praelux Incorporated | Procede et dispositif de criblage de composes chimiques |
| US7033819B2 (en) | 2000-11-08 | 2006-04-25 | Surface Logix, Inc. | System for monitoring cell motility in real-time |
| US20020132274A1 (en) | 2001-01-17 | 2002-09-19 | Nevalainen Marja T. | Diagnostic and monitorings methods for cancer |
| US6873733B2 (en) | 2001-01-19 | 2005-03-29 | The Regents Of The University Of Colorado | Combined wavefront coding and amplitude contrast imaging systems |
| AU2002324422A1 (en) | 2001-02-21 | 2002-12-23 | Amnis Corporation | Method and apparatus for labeling and analyzing cellular components |
| EP1368653B1 (fr) * | 2001-03-12 | 2013-01-23 | Cellomics, Inc. | Methodes pouvant augmenter la capacite d'essais de criblage cellulaire a grande densite |
| US20020126275A1 (en) | 2001-03-12 | 2002-09-12 | Johnson Paul E. | LED illuminated particle detection apparatus and methods |
| US20030048931A1 (en) | 2001-03-23 | 2003-03-13 | Peter Johnson | Quantification and differentiation of tissue based upon quantitative image analysis |
| US8050868B2 (en) | 2001-03-26 | 2011-11-01 | Cellomics, Inc. | Methods for determining the organization of a cellular component of interest |
| US6519355B2 (en) | 2001-03-28 | 2003-02-11 | Alan C. Nelson | Optical projection imaging system and method for automatically detecting cells having nuclear and cytoplasmic densitometric features associated with disease |
| US7050620B2 (en) | 2001-03-30 | 2006-05-23 | Heckman Carol A | Method of assaying shape and structural features in cells |
| AU2002303234A1 (en) * | 2001-04-02 | 2002-10-15 | Cytoprint, Inc. | Methods and apparatus for discovering, identifying and comparing biological activity mechanisms |
| CA2445044C (fr) | 2001-04-25 | 2011-02-15 | Amnis Corporation | Procede et appareil de correction de la diaphonie et de la resolution spatiale de l'imagerie multicanal |
| US6618140B2 (en) | 2001-06-18 | 2003-09-09 | Amnis Corporation | Spectral deconvolution of fluorescent markers |
| US7190832B2 (en) | 2001-07-17 | 2007-03-13 | Amnis Corporation | Computational methods for the segmentation of images of objects from background in a flow imaging instrument |
| EP1423506A4 (fr) | 2001-08-06 | 2008-08-06 | Univ Vanderbilt | Appareil et procedes permettant de discriminer un agent a l'aide de matiere biologique |
| US20030104439A1 (en) | 2001-11-30 | 2003-06-05 | Finch Rosalynde J. | Methods of identifying cellular target molecules |
| EP1316793A1 (fr) | 2001-12-03 | 2003-06-04 | Christian Leist | Procédé et appareil pour la détermination d'une collection de cellules |
| JP4497923B2 (ja) | 2001-12-05 | 2010-07-07 | ザ ジェイ. デビッド グラッドストーン インスティテューツ | ロボット顕微鏡検査システム |
| JP4249627B2 (ja) | 2001-12-18 | 2009-04-02 | ザ ユニバーシティ オブ ロチェスター | 多重焦点非球面レンズを使用して拡大焦点距離を得る撮像 |
| ES2379252T3 (es) | 2002-04-11 | 2012-04-24 | Cyclex, Inc. | Método para monitorizar la respuesta inmunitaria y predecir los desenlaces clínicos en receptores de trasplantes |
| US6658143B2 (en) * | 2002-04-29 | 2003-12-02 | Amersham Biosciences Corp. | Ray-based image analysis for biological specimens |
| US7378386B2 (en) * | 2002-07-15 | 2008-05-27 | Board Of Regents, The University Of Texas System | Anti-viral treatment methods using phosphatidylethanolamine-binding peptide derivatives |
| AU2003270687B2 (en) | 2002-09-13 | 2008-05-08 | Life Technologies Corporation | Interactive and automated tissue image analysis with global training database and variable-abstraction processing in cytological specimen classification and laser capture microdissection applications |
| US8741557B2 (en) | 2003-02-14 | 2014-06-03 | Beth Israel Deaconess Medical Center, Inc. | Predicting graft rejection |
| JP4585511B2 (ja) | 2003-03-27 | 2010-11-24 | バートロン メディカル イマジング、エルエルシー | 病原体、細菌および異常な細胞を迅速に同定するためのシステムおよび方法 |
| US7180673B2 (en) | 2003-03-28 | 2007-02-20 | Cdm Optics, Inc. | Mechanically-adjustable optical phase filters for modifying depth of field, aberration-tolerance, anti-aliasing in optical systems |
| US7042639B1 (en) | 2003-08-21 | 2006-05-09 | The United States Of America As Represented By The Administrator Of Nasa | Identification of cells with a compact microscope imaging system with intelligent controls |
| US7289205B2 (en) | 2003-09-19 | 2007-10-30 | The General Hospital Corporation | Fluorescence polarization imaging devices and methods |
| WO2005090945A1 (fr) | 2004-03-16 | 2005-09-29 | Amnis Corporation | Procede d'imagerie et d'analyse differentielle de cellules |
| ATE538138T1 (de) | 2004-03-16 | 2012-01-15 | Amnis Corp | Auf bildlicher darstellung beruhende quantifizierung molekularer translokation |
| US7907769B2 (en) * | 2004-05-13 | 2011-03-15 | The Charles Stark Draper Laboratory, Inc. | Image-based methods for measuring global nuclear patterns as epigenetic markers of cell differentiation |
| GB2416043B (en) | 2004-07-06 | 2008-02-06 | Cairn Res Ltd | Optical imaging device |
| US8003333B2 (en) | 2007-09-28 | 2011-08-23 | Mayo Foundation For Medical Education And Research | Serum biomarkers for early detection of acute cellular rejection |
| EP2271373B1 (fr) * | 2008-03-10 | 2018-10-31 | The Medical College Of Wisconsin, Inc. | Peptide contenant 19 acides aminés marqué avec 99mtc pour utilisation en tant que sonde moléculaire se liant à la phosphatidyléthanolamine et produit radiopharmaceutique |
-
2005
- 2005-03-16 AT AT05760334T patent/ATE538138T1/de active
- 2005-03-16 EP EP05760334A patent/EP1800124B1/fr not_active Expired - Lifetime
- 2005-03-16 CA CA002598602A patent/CA2598602A1/fr not_active Abandoned
- 2005-03-16 WO PCT/US2005/008866 patent/WO2005098430A2/fr not_active Ceased
- 2005-03-16 US US10/593,018 patent/US8150136B2/en active Active
-
2012
- 2012-03-30 US US13/436,371 patent/US9528989B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US20090202130A1 (en) | 2009-08-13 |
| EP1800124A2 (fr) | 2007-06-27 |
| WO2005098430A3 (fr) | 2005-12-15 |
| EP1800124B1 (fr) | 2011-12-21 |
| ATE538138T1 (de) | 2012-01-15 |
| US20120244550A1 (en) | 2012-09-27 |
| US9528989B2 (en) | 2016-12-27 |
| WO2005098430A2 (fr) | 2005-10-20 |
| US8150136B2 (en) | 2012-04-03 |
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