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CA2435631A1 - Procedes et compositions pour l'expression de polynucleotides specifiquement dans des cellules de muscle lisse in vivo - Google Patents

Procedes et compositions pour l'expression de polynucleotides specifiquement dans des cellules de muscle lisse in vivo Download PDF

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Publication number
CA2435631A1
CA2435631A1 CA002435631A CA2435631A CA2435631A1 CA 2435631 A1 CA2435631 A1 CA 2435631A1 CA 002435631 A CA002435631 A CA 002435631A CA 2435631 A CA2435631 A CA 2435631A CA 2435631 A1 CA2435631 A1 CA 2435631A1
Authority
CA
Canada
Prior art keywords
mhc
promoter
smooth muscle
seq
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002435631A
Other languages
English (en)
Inventor
Gary K. Owens
Ichiro Manabe
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Setagon Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2435631A1 publication Critical patent/CA2435631A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

L'invention concerne en règle générale des promoteurs, des amplificateurs et autres éléments régulateurs de cellules de muscle lisse. L'invention concerne plus particulièrement des procédés pour l'inactivation ou la surexpression ciblée de gènes visés, dans les cellules de muscle lisse ou dans un sous-type de ces cellules. L'invention concerne également des procédés pour l'expression de polynucléotides in vivo spécifiquement dans les cellules de muscle lisse ou dans des sous-types de ces cellules.
CA002435631A 2001-01-24 2002-01-24 Procedes et compositions pour l'expression de polynucleotides specifiquement dans des cellules de muscle lisse in vivo Abandoned CA2435631A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US26381101P 2001-01-24 2001-01-24
US60/263,811 2001-01-24
PCT/US2002/002016 WO2002059270A2 (fr) 2001-01-24 2002-01-24 Procedes et compositions pour l'expression de polynucleotides specifiquement dans des cellules de muscle lisse in vivo

Publications (1)

Publication Number Publication Date
CA2435631A1 true CA2435631A1 (fr) 2002-08-01

Family

ID=23003314

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002435631A Abandoned CA2435631A1 (fr) 2001-01-24 2002-01-24 Procedes et compositions pour l'expression de polynucleotides specifiquement dans des cellules de muscle lisse in vivo

Country Status (4)

Country Link
EP (1) EP1360191A4 (fr)
AU (1) AU2002237928A1 (fr)
CA (1) CA2435631A1 (fr)
WO (1) WO2002059270A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1379643A4 (fr) * 2001-03-20 2004-07-21 Univ Virginia Procedes d'identification et de purification de cellules souches de muscle lisse
CN102676527B (zh) * 2012-05-29 2013-09-25 山东农业大学 一种骨骼肌特异性MYHC-IIx启动子及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9305091D0 (en) * 1993-03-12 1993-04-28 Thrombosis Res Inst Eukaryotic expression vectors driven by a novel strong mammalian cell promoter
DE19620308A1 (de) * 1996-05-10 1997-11-13 Franz Wolfgang M Dr Vektor-System zur spezifischen in vivo Genexpression in glatten Gefäßmuskelzellen
AU2325099A (en) * 1998-01-16 1999-08-02 University Of Virginia Patent Foundation Identification of a smooth muscle cell (smc) specific smooth muscle myosin heavychain (sm-mhc) promoter/enhancer

Also Published As

Publication number Publication date
WO2002059270A8 (fr) 2003-11-06
WO2002059270A2 (fr) 2002-08-01
AU2002237928A1 (en) 2002-08-06
EP1360191A4 (fr) 2004-08-25
EP1360191A2 (fr) 2003-11-12
WO2002059270A3 (fr) 2003-03-27

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Legal Events

Date Code Title Description
FZDE Discontinued