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CA2432346A1 - Emplacement et fonction de proteines de liaison a l'adn a l'echelle du genome - Google Patents

Emplacement et fonction de proteines de liaison a l'adn a l'echelle du genome Download PDF

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Publication number
CA2432346A1
CA2432346A1 CA002432346A CA2432346A CA2432346A1 CA 2432346 A1 CA2432346 A1 CA 2432346A1 CA 002432346 A CA002432346 A CA 002432346A CA 2432346 A CA2432346 A CA 2432346A CA 2432346 A1 CA2432346 A1 CA 2432346A1
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CA
Canada
Prior art keywords
dna
cell
protein
genes
interest
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002432346A
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English (en)
Inventor
John Wyrick
Richard A. Young
Bing Ren
Francois Robert
Itamar Simon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Whitehead Institute for Biomedical Research
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Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2432346A1 publication Critical patent/CA2432346A1/fr
Abandoned legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La présente invention porte sur un procédé d'identification d'une région (ou de plusieurs régions) d'un génome d'une cellule à laquelle (auxquelles) est liée une protéine. Selon les procédés décrits, la protéine de liaison à l'ADN d'une cellule est liée (c'est-à-dire réticulée de manière covalente) à l'ADN génomique d'une cellule. L'ADN génomique auquel la protéine de liaison est liée est retiré et combiné, ou mis en contact avec un ADN comprenant une séquence complémentaire à l'ADN génomique de la cellule dans des conditions où se produit l'hybridation entre l'ADN génomique identifié et la séquence complémentaire à l'ADN génomique. La ou les régions d'hybridation constituent la ou les régions du génome de la cellule où se lie la protéine. L'invention concerne également un procédé d'identification d'un ensemble de gènes où la liaison régulatrice de cycle cellulaire est en corrélation avec l'expression génique, ainsi qu'un procédé d'identification de cibles génomiques d'activateurs transcriptionnels de cycle cellulaire de cellules vivantes.
CA002432346A 2000-12-21 2001-12-21 Emplacement et fonction de proteines de liaison a l'adn a l'echelle du genome Abandoned CA2432346A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US25745500P 2000-12-21 2000-12-21
US60/257,455 2000-12-21
US32362001P 2001-09-20 2001-09-20
US60/323,620 2001-09-20
PCT/US2001/050396 WO2002059371A2 (fr) 2000-12-21 2001-12-21 Emplacement et fonction de proteines de liaison a l'adn a l'echelle du genome

Publications (1)

Publication Number Publication Date
CA2432346A1 true CA2432346A1 (fr) 2002-08-01

Family

ID=26945967

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002432346A Abandoned CA2432346A1 (fr) 2000-12-21 2001-12-21 Emplacement et fonction de proteines de liaison a l'adn a l'echelle du genome

Country Status (3)

Country Link
EP (1) EP1381692A2 (fr)
CA (1) CA2432346A1 (fr)
WO (1) WO2002059371A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006078313A (ja) * 2004-09-09 2006-03-23 Hitachi Software Eng Co Ltd タンパク質の結合位置同定方法
US9411930B2 (en) * 2013-02-01 2016-08-09 The Regents Of The University Of California Methods for genome assembly and haplotype phasing

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1220949B1 (fr) * 1999-09-01 2011-03-30 Whitehead Institute For Biomedical Research Analyse s'etendant aux chromosomes des interactions entre une proteine et l'adn

Also Published As

Publication number Publication date
WO2002059371A2 (fr) 2002-08-01
WO2002059371B1 (fr) 2003-12-18
EP1381692A2 (fr) 2004-01-21
WO2002059371A3 (fr) 2003-11-06

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CA2432346A1 (fr) Emplacement et fonction de proteines de liaison a l'adn a l'echelle du genome
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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued