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CA2431640A1 - P450rai-2 (p450 cytochrome 26b), codage de molecules d'acides nucleiques et leurs methodes d'utilisation - Google Patents

P450rai-2 (p450 cytochrome 26b), codage de molecules d'acides nucleiques et leurs methodes d'utilisation Download PDF

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Publication number
CA2431640A1
CA2431640A1 CA 2431640 CA2431640A CA2431640A1 CA 2431640 A1 CA2431640 A1 CA 2431640A1 CA 2431640 CA2431640 CA 2431640 CA 2431640 A CA2431640 A CA 2431640A CA 2431640 A1 CA2431640 A1 CA 2431640A1
Authority
CA
Canada
Prior art keywords
p450rai
protein
nucleic acid
cells
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2431640
Other languages
English (en)
Inventor
Jay A. White
P. Martin Petkovich
Glenville Jones
Heather Ramshaw
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytochroma Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/CA2000/001493 external-priority patent/WO2001044443A2/fr
Application filed by Individual filed Critical Individual
Priority to CA 2431640 priority Critical patent/CA2431640A1/fr
Priority claimed from PCT/CA2001/001805 external-priority patent/WO2002048334A2/fr
Publication of CA2431640A1 publication Critical patent/CA2431640A1/fr
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Oncology (AREA)
  • Cell Biology (AREA)
  • Hospice & Palliative Care (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un nouvel acide tout-trans rétinoïque inductible d'acide tout-trans rétinoïque métabolisant le cytochrome P450, appelé P450RAI-2, qui est principalement exprimé dans le cerveau, en particulier dans le cervelet. Il est également exprimé dans les tissus pulmonaires sains ou tumoraux ainsi que dans les cellules du cancer du sein et peut avoir une corrélation avec le cancer des poumons et du sein. Le P450RAI-2 humain présente une homologie d'acides aminés d'au moins 42 % avec le P450RAI-1 humain. Lorsqu'il est transfecté dans des cellules COS-1, il entraîne une conversion rapide de tous les acides tout-trans rétinoïques dans plus de métabolites polaires comprenant les produits inactifs 4-oxo-RA, 4-OH-RA et 18-OH-RA. De plus, P450RAI-2, comme P450RAI-1, est aussi inductible dans certaines lignées de cellules de culture exposées à l'acide tout-trans rétinoïque. Par ailleurs, l'invention concerne des méthodes d'utilisation de ces nouveaux polynucléotide, polypeptide, fragments et inhibiteurs de ceux-ci, notamment dans le traitement de troubles dermatologiques, du cancer et de certains troubles cérébraux.
CA 2431640 2000-12-15 2001-12-17 P450rai-2 (p450 cytochrome 26b), codage de molecules d'acides nucleiques et leurs methodes d'utilisation Abandoned CA2431640A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA 2431640 CA2431640A1 (fr) 2000-12-15 2001-12-17 P450rai-2 (p450 cytochrome 26b), codage de molecules d'acides nucleiques et leurs methodes d'utilisation

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CAPCT/CA00/01493 2000-12-15
PCT/CA2000/001493 WO2001044443A2 (fr) 1999-12-16 2000-12-15 Proteine p450rai-2 et proteines associees
CA 2431640 CA2431640A1 (fr) 2000-12-15 2001-12-17 P450rai-2 (p450 cytochrome 26b), codage de molecules d'acides nucleiques et leurs methodes d'utilisation
PCT/CA2001/001805 WO2002048334A2 (fr) 2000-12-15 2001-12-17 P450rai-2 (p450 cytochrome 26b), codage de molecules d'acides nucleiques et leurs methodes d'utilisation

Publications (1)

Publication Number Publication Date
CA2431640A1 true CA2431640A1 (fr) 2002-06-20

Family

ID=27805845

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2431640 Abandoned CA2431640A1 (fr) 2000-12-15 2001-12-17 P450rai-2 (p450 cytochrome 26b), codage de molecules d'acides nucleiques et leurs methodes d'utilisation

Country Status (1)

Country Link
CA (1) CA2431640A1 (fr)

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued