CA2426812A1 - Detection de sequences nucleotidiques specifiques - Google Patents
Detection de sequences nucleotidiques specifiques Download PDFInfo
- Publication number
- CA2426812A1 CA2426812A1 CA002426812A CA2426812A CA2426812A1 CA 2426812 A1 CA2426812 A1 CA 2426812A1 CA 002426812 A CA002426812 A CA 002426812A CA 2426812 A CA2426812 A CA 2426812A CA 2426812 A1 CA2426812 A1 CA 2426812A1
- Authority
- CA
- Canada
- Prior art keywords
- oligonucleotide
- strand
- nucleotide sequence
- extended
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La présente invention se rapporte à des amorces oligonucléotidiques, marquées chacune au moyen d'une paire assurant un transfert d'énergie moléculaire, ladite paire comprenant un donneur d'énergie et un accepteur d'énergie. Lesdites amorces oligonucléotidiques sont utilisées pour détecter la présence d'une ou de plusieurs séquences nucléotidiques d'identification. Deux amorces oligonucléotidiques spécifiques d'une séquence, l'une spécifique d'une première séquence d'identification et l'autre de la deuxième séquence d'identification, ainsi qu'une amorce inverse, sont employées dans un mélange produit par réaction en chaîne de la polymérase (PCR) qui contient une matrice d'acide nucléique. En utilisant seulement sa séquence d'identification complémentaire comme matrice, chaque amorce spécifique d'une séquence dirige l'amplification. Les produits de la PCR qui en résultent, représentant la première et la deuxième séquence d'identification, sont détectés par amplification PCR qui utilise deux amorces marquées par transfert d'énergie moléculaire, chacune étant spécifique du produit de la PCR représentant soit la première, soit la deuxième séquence d'identification, ainsi qu'une autre amorce qui est complémentaire des produits de la PCR représentant les deux séquences d'identification.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US24267200P | 2000-10-24 | 2000-10-24 | |
| US60/242,672 | 2000-10-24 | ||
| PCT/US2001/032630 WO2002034947A2 (fr) | 2000-10-24 | 2001-10-24 | Detection de sequences nucleotidiques specifiques |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2426812A1 true CA2426812A1 (fr) | 2002-05-02 |
Family
ID=22915737
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002426812A Abandoned CA2426812A1 (fr) | 2000-10-24 | 2001-10-24 | Detection de sequences nucleotidiques specifiques |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP1354063A2 (fr) |
| JP (1) | JP2004530410A (fr) |
| AU (1) | AU2002213416A1 (fr) |
| CA (1) | CA2426812A1 (fr) |
| WO (1) | WO2002034947A2 (fr) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030170666A1 (en) * | 2001-08-21 | 2003-09-11 | The Secretary Of State For The Home | Amplification |
| IES20150206A2 (en) | 2014-07-14 | 2016-03-09 | Identigen Ltd | A method for detecting PCR amplification in a sample |
| EP2975136A1 (fr) * | 2014-07-14 | 2016-01-20 | Parlanca Limited | Procédé de détection de l'amplification par PCR dans un échantillon |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2176348C (fr) * | 1993-11-12 | 2004-11-02 | Sanjay Tyagi | Sondes d'hybridation pour la detection d'acides nucleiques, souches universelles, methodes et materiels |
| US6117635A (en) * | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| EP1036201A2 (fr) * | 1997-12-12 | 2000-09-20 | Intergen Company | Methode de detection de l'apoptose a l'aide d'oligonucleotides marques par fret |
-
2001
- 2001-10-24 AU AU2002213416A patent/AU2002213416A1/en not_active Abandoned
- 2001-10-24 WO PCT/US2001/032630 patent/WO2002034947A2/fr not_active Ceased
- 2001-10-24 JP JP2002537915A patent/JP2004530410A/ja active Pending
- 2001-10-24 CA CA002426812A patent/CA2426812A1/fr not_active Abandoned
- 2001-10-24 EP EP01981799A patent/EP1354063A2/fr not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| AU2002213416A1 (en) | 2002-05-06 |
| JP2004530410A (ja) | 2004-10-07 |
| WO2002034947A2 (fr) | 2002-05-02 |
| WO2002034947A3 (fr) | 2003-08-14 |
| EP1354063A2 (fr) | 2003-10-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Dead |