CA2408850A1 - Dosage de fonction plaquettaire et reactif concu a cet effet - Google Patents
Dosage de fonction plaquettaire et reactif concu a cet effet Download PDFInfo
- Publication number
- CA2408850A1 CA2408850A1 CA002408850A CA2408850A CA2408850A1 CA 2408850 A1 CA2408850 A1 CA 2408850A1 CA 002408850 A CA002408850 A CA 002408850A CA 2408850 A CA2408850 A CA 2408850A CA 2408850 A1 CA2408850 A1 CA 2408850A1
- Authority
- CA
- Canada
- Prior art keywords
- ligand
- platelet
- platelet function
- piop
- von willebrand
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000003556 assay Methods 0.000 title claims abstract description 68
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 48
- 210000001772 blood platelet Anatomy 0.000 claims abstract description 142
- 239000003446 ligand Substances 0.000 claims abstract description 69
- 230000005291 magnetic effect Effects 0.000 claims abstract description 56
- 239000006249 magnetic particle Substances 0.000 claims abstract description 41
- 239000000203 mixture Substances 0.000 claims abstract description 27
- 230000003993 interaction Effects 0.000 claims abstract description 25
- 230000002776 aggregation Effects 0.000 claims abstract description 24
- 238000004220 aggregation Methods 0.000 claims abstract description 24
- 239000008280 blood Substances 0.000 claims description 33
- 210000004369 blood Anatomy 0.000 claims description 26
- 238000000034 method Methods 0.000 claims description 23
- 108010047303 von Willebrand Factor Proteins 0.000 claims description 21
- 102100036537 von Willebrand factor Human genes 0.000 claims description 21
- 229960001134 von willebrand factor Drugs 0.000 claims description 20
- 239000012634 fragment Substances 0.000 claims description 18
- 210000002381 plasma Anatomy 0.000 claims description 14
- 108010035532 Collagen Proteins 0.000 claims description 11
- 102000008186 Collagen Human genes 0.000 claims description 11
- 229920001436 collagen Polymers 0.000 claims description 11
- 230000033001 locomotion Effects 0.000 claims description 11
- 108090000190 Thrombin Proteins 0.000 claims description 10
- 230000004044 response Effects 0.000 claims description 10
- 229960004072 thrombin Drugs 0.000 claims description 10
- 238000012544 monitoring process Methods 0.000 claims description 8
- 210000004623 platelet-rich plasma Anatomy 0.000 claims description 8
- 229960005188 collagen Drugs 0.000 claims 5
- 238000012360 testing method Methods 0.000 description 85
- 239000000523 sample Substances 0.000 description 41
- 108020003175 receptors Proteins 0.000 description 40
- 102000005962 receptors Human genes 0.000 description 40
- 238000006243 chemical reaction Methods 0.000 description 37
- 239000004793 Polystyrene Substances 0.000 description 32
- 239000011324 bead Substances 0.000 description 32
- 239000002245 particle Substances 0.000 description 32
- 108010035030 Platelet Membrane Glycoprotein IIb Proteins 0.000 description 26
- 229920002223 polystyrene Polymers 0.000 description 26
- 238000009472 formulation Methods 0.000 description 22
- 229940107685 reopro Drugs 0.000 description 22
- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 22
- 239000007787 solid Substances 0.000 description 15
- 238000002847 impedance measurement Methods 0.000 description 14
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 12
- 229940098773 bovine serum albumin Drugs 0.000 description 12
- 108010049003 Fibrinogen Proteins 0.000 description 11
- 102000008946 Fibrinogen Human genes 0.000 description 11
- 239000003814 drug Substances 0.000 description 11
- 229940012952 fibrinogen Drugs 0.000 description 11
- 238000005259 measurement Methods 0.000 description 11
- 239000000556 agonist Substances 0.000 description 10
- 239000007790 solid phase Substances 0.000 description 10
- 230000004913 activation Effects 0.000 description 9
- 239000000463 material Substances 0.000 description 9
- 229940079593 drug Drugs 0.000 description 8
- 102000009123 Fibrin Human genes 0.000 description 7
- 108010073385 Fibrin Proteins 0.000 description 7
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 7
- 230000008859 change Effects 0.000 description 7
- 238000001514 detection method Methods 0.000 description 7
- 229950003499 fibrin Drugs 0.000 description 7
- BSYNRYMUTXBXSQ-UHFFFAOYSA-N Aspirin Chemical compound CC(=O)OC1=CC=CC=C1C(O)=O BSYNRYMUTXBXSQ-UHFFFAOYSA-N 0.000 description 6
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N Iron oxide Chemical compound [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 description 6
- 239000000872 buffer Substances 0.000 description 6
- 230000015271 coagulation Effects 0.000 description 6
- 238000005345 coagulation Methods 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 229920000669 heparin Polymers 0.000 description 5
- 239000003112 inhibitor Substances 0.000 description 5
- 238000002483 medication Methods 0.000 description 5
- 229910052751 metal Inorganic materials 0.000 description 5
- 239000002184 metal Substances 0.000 description 5
- 125000006850 spacer group Chemical group 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 4
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 4
- 108090000288 Glycoproteins Proteins 0.000 description 4
- 102000003886 Glycoproteins Human genes 0.000 description 4
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 4
- 239000012190 activator Substances 0.000 description 4
- 239000005557 antagonist Substances 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 230000035602 clotting Effects 0.000 description 4
- 230000001419 dependent effect Effects 0.000 description 4
- 210000002744 extracellular matrix Anatomy 0.000 description 4
- 229960002897 heparin Drugs 0.000 description 4
- 238000006116 polymerization reaction Methods 0.000 description 4
- 230000002829 reductive effect Effects 0.000 description 4
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 3
- 206010053567 Coagulopathies Diseases 0.000 description 3
- HEFNNWSXXWATRW-UHFFFAOYSA-N Ibuprofen Chemical compound CC(C)CC1=CC=C(C(C)C(O)=O)C=C1 HEFNNWSXXWATRW-UHFFFAOYSA-N 0.000 description 3
- 239000007983 Tris buffer Substances 0.000 description 3
- 208000024248 Vascular System injury Diseases 0.000 description 3
- 208000012339 Vascular injury Diseases 0.000 description 3
- 229910052782 aluminium Inorganic materials 0.000 description 3
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 3
- 239000003146 anticoagulant agent Substances 0.000 description 3
- 230000023597 hemostasis Effects 0.000 description 3
- 229960001680 ibuprofen Drugs 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 239000004005 microsphere Substances 0.000 description 3
- 230000005298 paramagnetic effect Effects 0.000 description 3
- 230000010118 platelet activation Effects 0.000 description 3
- 230000002035 prolonged effect Effects 0.000 description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 2
- 206010002388 Angina unstable Diseases 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 108010054218 Factor VIII Proteins 0.000 description 2
- 102000001690 Factor VIII Human genes 0.000 description 2
- 208000009292 Hemophilia A Diseases 0.000 description 2
- 108010010336 Platelet Membrane Glycoproteins Proteins 0.000 description 2
- 102000015795 Platelet Membrane Glycoproteins Human genes 0.000 description 2
- 230000010799 Receptor Interactions Effects 0.000 description 2
- 208000007814 Unstable Angina Diseases 0.000 description 2
- UCTWMZQNUQWSLP-UHFFFAOYSA-N adrenaline Chemical compound CNCC(O)C1=CC=C(O)C(O)=C1 UCTWMZQNUQWSLP-UHFFFAOYSA-N 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 229940127219 anticoagulant drug Drugs 0.000 description 2
- YZXBAPSDXZZRGB-DOFZRALJSA-N arachidonic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YZXBAPSDXZZRGB-DOFZRALJSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000002981 blocking agent Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000008602 contraction Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 229960000301 factor viii Drugs 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 238000011049 filling Methods 0.000 description 2
- 201000004332 intermediate coronary syndrome Diseases 0.000 description 2
- SZVJSHCCFOBDDC-UHFFFAOYSA-N iron(II,III) oxide Inorganic materials O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 230000037361 pathway Effects 0.000 description 2
- 230000003389 potentiating effect Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 239000003381 stabilizer Substances 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 238000007704 wet chemistry method Methods 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- ABEXEQSGABRUHS-UHFFFAOYSA-N 16-methylheptadecyl 16-methylheptadecanoate Chemical compound CC(C)CCCCCCCCCCCCCCCOC(=O)CCCCCCCCCCCCCCC(C)C ABEXEQSGABRUHS-UHFFFAOYSA-N 0.000 description 1
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 208000033316 Acquired hemophilia A Diseases 0.000 description 1
- 102100035767 Adrenocortical dysplasia protein homolog Human genes 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 102100026735 Coagulation factor VIII Human genes 0.000 description 1
- 108010056764 Eptifibatide Proteins 0.000 description 1
- 201000003542 Factor VIII deficiency Diseases 0.000 description 1
- 208000031220 Hemophilia Diseases 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 101100433963 Homo sapiens ACD gene Proteins 0.000 description 1
- 101000911390 Homo sapiens Coagulation factor VIII Proteins 0.000 description 1
- 241000764238 Isis Species 0.000 description 1
- KLDXJTOLSGUMSJ-JGWLITMVSA-N Isosorbide Chemical compound O[C@@H]1CO[C@@H]2[C@@H](O)CO[C@@H]21 KLDXJTOLSGUMSJ-JGWLITMVSA-N 0.000 description 1
- CMWTZPSULFXXJA-UHFFFAOYSA-N Naproxen Natural products C1=C(C(C)C(O)=O)C=CC2=CC(OC)=CC=C21 CMWTZPSULFXXJA-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 108010081391 Ristocetin Proteins 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 229960001138 acetylsalicylic acid Drugs 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 206010000891 acute myocardial infarction Diseases 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 230000004520 agglutination Effects 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- 230000003556 anti-epileptic effect Effects 0.000 description 1
- 239000001961 anticonvulsive agent Substances 0.000 description 1
- 229960003965 antiepileptics Drugs 0.000 description 1
- 229940030225 antihemorrhagics Drugs 0.000 description 1
- 229940127218 antiplatelet drug Drugs 0.000 description 1
- 229940114079 arachidonic acid Drugs 0.000 description 1
- 235000021342 arachidonic acid Nutrition 0.000 description 1
- 108010083526 asialo-von Willebrand Factor Proteins 0.000 description 1
- 238000011948 assay development Methods 0.000 description 1
- 238000013096 assay test Methods 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- 208000034158 bleeding Diseases 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 239000004067 bulking agent Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- BGTFCAQCKWKTRL-YDEUACAXSA-N chembl1095986 Chemical compound C1[C@@H](N)[C@@H](O)[C@H](C)O[C@H]1O[C@@H]([C@H]1C(N[C@H](C2=CC(O)=CC(O[C@@H]3[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O)=C2C=2C(O)=CC=C(C=2)[C@@H](NC(=O)[C@@H]2NC(=O)[C@@H]3C=4C=C(C(=C(O)C=4)C)OC=4C(O)=CC=C(C=4)[C@@H](N)C(=O)N[C@@H](C(=O)N3)[C@H](O)C=3C=CC(O4)=CC=3)C(=O)N1)C(O)=O)=O)C(C=C1)=CC=C1OC1=C(O[C@@H]3[C@H]([C@H](O)[C@@H](O)[C@H](CO[C@@H]5[C@H]([C@@H](O)[C@H](O)[C@@H](C)O5)O)O3)O[C@@H]3[C@H]([C@@H](O)[C@H](O)[C@@H](CO)O3)O[C@@H]3[C@H]([C@H](O)[C@@H](CO)O3)O)C4=CC2=C1 BGTFCAQCKWKTRL-YDEUACAXSA-N 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229960004468 eptifibatide Drugs 0.000 description 1
- GLGOPUHVAZCPRB-LROMGURASA-N eptifibatide Chemical compound N1C(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CCCCNC(=N)N)NC(=O)CCSSC[C@@H](C(N)=O)NC(=O)[C@@H]2CCCN2C(=O)[C@@H]1CC1=CN=C2[C]1C=CC=C2 GLGOPUHVAZCPRB-LROMGURASA-N 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000000025 haemostatic effect Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000005417 image-selected in vivo spectroscopy Methods 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000012739 integrated shape imaging system Methods 0.000 description 1
- 230000000302 ischemic effect Effects 0.000 description 1
- 229960002479 isosorbide Drugs 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000000696 magnetic material Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000865 mononuclear phagocyte system Anatomy 0.000 description 1
- 229960002009 naproxen Drugs 0.000 description 1
- CMWTZPSULFXXJA-VIFPVBQESA-N naproxen Chemical compound C1=C([C@H](C)C(O)=O)C=CC2=CC(OC)=CC=C21 CMWTZPSULFXXJA-VIFPVBQESA-N 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 229960005489 paracetamol Drugs 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 238000013146 percutaneous coronary intervention Methods 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 230000003285 pharmacodynamic effect Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000002953 phosphate buffered saline Substances 0.000 description 1
- 230000006461 physiological response Effects 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 239000000106 platelet aggregation inhibitor Substances 0.000 description 1
- -1 polypropylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000007115 recruitment Effects 0.000 description 1
- 238000002310 reflectometry Methods 0.000 description 1
- 229950004257 ristocetin Drugs 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000008227 sterile water for injection Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- VCKUSRYTPJJLNI-UHFFFAOYSA-N terazosin Chemical compound N=1C(N)=C2C=C(OC)C(OC)=CC2=NC=1N(CC1)CCN1C(=O)C1CCCO1 VCKUSRYTPJJLNI-UHFFFAOYSA-N 0.000 description 1
- 229960001693 terazosin Drugs 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 230000002537 thrombolytic effect Effects 0.000 description 1
- 229960003425 tirofiban Drugs 0.000 description 1
- COKMIXFXJJXBQG-NRFANRHFSA-N tirofiban Chemical compound C1=CC(C[C@H](NS(=O)(=O)CCCC)C(O)=O)=CC=C1OCCCCC1CCNCC1 COKMIXFXJJXBQG-NRFANRHFSA-N 0.000 description 1
- 230000014599 transmission of virus Effects 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- 230000003827 upregulation Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
- G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
L'invention concerne un réactif de dosage d'une fonction plaquettaire, destiné à l'exécution de ce dosage et contenant un mélange de particules magnétiques et non magnétiques. Ce réactif est caractérisé en ce que sur la surface extérieure des particules magnétiques se trouve fixée une certaine quantité d'un premier ligand possédant une affinité pour une interaction directe avec les récepteurs de la glycoprotéine Ib (GP-Ib) sur des plaquettes sanguines, et en ce que sur la surface extérieure des particules non magnétiques se trouve fixée une certaine quantité d'un second ligand possédant une affinité pour une interaction directe avec des récepteurs de GP-Ib sur des plaquettes sanguines, de sorte que l'interaction de l'un des ligands -le premier ou le second- avec le récepteur plaquettaire de GP-Ib, provoque l'activation des plaquettes sanguines afin que celles-ci s'agrègent, le premier et le second ligand pouvant être semblables ou différents. L'invention concerne également un dosage mettant en oeuvre un tel réactif et destiné à produire une évaluation rapide, fiable, du point d'intervention de la fonction plaquettaire.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US20263800P | 2000-05-09 | 2000-05-09 | |
| US60/202,638 | 2000-05-09 | ||
| PCT/US2001/011760 WO2001086248A2 (fr) | 2000-05-09 | 2001-05-09 | Dosage de fonction plaquettaire et reactif concu a cet effet |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2408850A1 true CA2408850A1 (fr) | 2001-11-15 |
Family
ID=22750704
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002408850A Abandoned CA2408850A1 (fr) | 2000-05-09 | 2001-05-09 | Dosage de fonction plaquettaire et reactif concu a cet effet |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP1280931A4 (fr) |
| JP (1) | JP2003532891A (fr) |
| KR (1) | KR20030014219A (fr) |
| AU (1) | AU2001261020A1 (fr) |
| BR (1) | BR0110735A (fr) |
| CA (1) | CA2408850A1 (fr) |
| MX (1) | MXPA02011017A (fr) |
| NO (1) | NO20025300L (fr) |
| WO (1) | WO2001086248A2 (fr) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6680177B2 (en) * | 2001-12-07 | 2004-01-20 | Cardiovascular Diagnostics, Inc. | Low molecular weight heparin assay, system and reagent therefor |
| JP4078247B2 (ja) * | 2003-05-02 | 2008-04-23 | キヤノン株式会社 | 磁性体−生体物質複合体型構造体、磁性体に対して結合能を有するアミノ酸配列を有するペプチド断片及びその遺伝子、ならびに磁性体−生体物質複合体型構造体の製造方法 |
| WO2011135397A1 (fr) * | 2010-04-29 | 2011-11-03 | University Of Calcutta | Antiagrégant plaquettaire et procédés d'utilisation de celui-ci |
| KR101276179B1 (ko) * | 2011-08-08 | 2013-06-18 | 고려대학교 산학협력단 | 혈소판 복합기능 검사 장치 및 방법, 이에 구비되는 미세교반칩 |
| US8772040B2 (en) | 2012-08-31 | 2014-07-08 | Korea University Research And Business Foundation | Apparatus and method of platelet multi-function analysis, and micro stirring chip |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4849340A (en) * | 1987-04-03 | 1989-07-18 | Cardiovascular Diagnostics, Inc. | Reaction system element and method for performing prothrombin time assay |
| US5110727A (en) * | 1987-04-03 | 1992-05-05 | Cardiovascular Diagnostics, Inc. | Method for performing coagulation assays accurately, rapidly and simply, using dry chemical reagents and paramagnetic particles |
| TW221493B (fr) * | 1990-07-10 | 1994-03-01 | Cardiovascular Diagnostics Inc | |
| US5616311A (en) * | 1991-01-15 | 1997-04-01 | Hemosphere, Inc. | Non-crosslinked protein particles for therapeutic and diagnostic use |
| US5446132A (en) * | 1992-10-01 | 1995-08-29 | The General Hospital Corporation | Thrombin-activated platelet protein-2 (TAPP-2) |
| US5427913A (en) * | 1992-12-03 | 1995-06-27 | Alberta Cancer Board | Methods for determining platelet function |
| JPH08507148A (ja) * | 1993-02-17 | 1996-07-30 | カーディオヴァスキュラー ダイアグノスティクス，インク． | 固相化学カスケードイムノアッセイおよびアフィニティアッセイ |
| US5686597A (en) * | 1995-06-06 | 1997-11-11 | Incyte Pharmaceuticals, Inc. | Thrombin receptor homolog |
| US5922551A (en) * | 1997-03-20 | 1999-07-13 | Accumetrics, Inc. | Agglutrimetric platelet binding assays in blood |
| US6046051A (en) * | 1997-06-27 | 2000-04-04 | Hemosense, Inc. | Method and device for measuring blood coagulation or lysis by viscosity changes |
| US5932445A (en) * | 1997-11-07 | 1999-08-03 | Incyte Pharmaceuticals, Inc. | Signal peptide-containing proteins |
-
2001
- 2001-05-09 JP JP2001583144A patent/JP2003532891A/ja active Pending
- 2001-05-09 CA CA002408850A patent/CA2408850A1/fr not_active Abandoned
- 2001-05-09 MX MXPA02011017A patent/MXPA02011017A/es unknown
- 2001-05-09 EP EP01934872A patent/EP1280931A4/fr not_active Withdrawn
- 2001-05-09 KR KR1020027014988A patent/KR20030014219A/ko not_active Withdrawn
- 2001-05-09 BR BRPI0110735-6A patent/BR0110735A/pt unknown
- 2001-05-09 WO PCT/US2001/011760 patent/WO2001086248A2/fr not_active Ceased
- 2001-05-09 AU AU2001261020A patent/AU2001261020A1/en not_active Abandoned
-
2002
- 2002-11-05 NO NO20025300A patent/NO20025300L/no not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| BR0110735A (pt) | 2007-05-08 |
| EP1280931A4 (fr) | 2005-02-09 |
| KR20030014219A (ko) | 2003-02-15 |
| NO20025300L (no) | 2003-01-07 |
| NO20025300D0 (no) | 2002-11-05 |
| WO2001086248A3 (fr) | 2002-02-28 |
| AU2001261020A1 (en) | 2001-11-20 |
| EP1280931A2 (fr) | 2003-02-05 |
| JP2003532891A (ja) | 2003-11-05 |
| WO2001086248A2 (fr) | 2001-11-15 |
| MXPA02011017A (es) | 2004-08-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US6586259B1 (en) | Platelet/leukocyte interaction assay and reagent therefor | |
| KR100351602B1 (ko) | 정확,신속및간결한피브리노겐분석을위한개선된방법및분석시스템 | |
| CA2087033C (fr) | Methodes de dosage de la fibrinogene a l'aide de reactifs secs renfermant des particules magnetiques | |
| Laffan et al. | Investigation of haemostasis | |
| Varon et al. | Cone and plate (let) analyzer: monitoring glycoprotein IIb/IIIa antagonists and von Willebrand disease replacement therapy by testing platelet deposition under flow conditions | |
| EP2153235B1 (fr) | Procédés de mesure de l'inhibition de l'agrégation plaquettaire par des antagonistes des récepteurs de la thrombine | |
| US20030044871A1 (en) | Coagulation assay reagents containing lanthanides and a protein C assay using such a lanthanide-containing reagent | |
| US20030211551A1 (en) | Platelet function assay and reagent therefor | |
| CA2408850A1 (fr) | Dosage de fonction plaquettaire et reactif concu a cet effet | |
| Stevens et al. | Evaluation of three methods for plasma fibrinogen determination | |
| JP2004506200A (ja) | ＧＰＩＩｂ／ＩＩＩａレセプターアンタゴニストの簡易薬剤監視法 | |
| JP3095608B2 (ja) | 血液凝固時間測定乾燥試薬 | |
| Laugen et al. | Hereditary dysfibrinogenemia characterized by slow fibrinopeptide release and competitive inhibition of thrombin | |
| Abdullah et al. | Practical Haemostasisfor Medical Personnel | |
| Laffan et al. | 16 Investigation of haemostasis | |
| van der Vorm et al. | Supplementary Methods | |
| Ledford-Kraemer | Von Willebrand Factor (VWF) Testing Von Willebrand Factor (VWF) Testing |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Dead |