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CA2407714A1 - Expression de la proteine de fixation d'heparine dans des cellules mammiferes de recombinaison - Google Patents

Expression de la proteine de fixation d'heparine dans des cellules mammiferes de recombinaison Download PDF

Info

Publication number
CA2407714A1
CA2407714A1 CA002407714A CA2407714A CA2407714A1 CA 2407714 A1 CA2407714 A1 CA 2407714A1 CA 002407714 A CA002407714 A CA 002407714A CA 2407714 A CA2407714 A CA 2407714A CA 2407714 A1 CA2407714 A1 CA 2407714A1
Authority
CA
Canada
Prior art keywords
arg
leu
gly
hbp
pro
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002407714A
Other languages
English (en)
Inventor
Hans Jakob Flodgaard
Poul Baad Rasmussen
Soren Bjorn
Ivan Svendsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Leukotech AS
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2407714A1 publication Critical patent/CA2407714A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4723—Cationic antimicrobial peptides, e.g. defensins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention concerne un procédé servant à produire une protéine mammifère de fixation d'héparine (HBP) dans une cellule mammifère qu'on peut cultiver dans des conditions anaérobies après avoir introduit un acide nucléique codant une protéine de fixation d'héparine dans ladite cellule, ce qui consiste à : (a) introduire dans ladite cellule mammifère un acide nucléique codant ladite protéine de fixation d'héparine ; (b) cultiver la cellule de l'étape (a) dans des conditions favorables au déclenchement de l'expression de ladite HBP ; (c) extraire ladite HBP du milieu de culture. Elle concerne, de plus, la cellule mammifère de recombinaison mise en application dans ce procédé.
CA002407714A 1999-04-29 2000-04-28 Expression de la proteine de fixation d'heparine dans des cellules mammiferes de recombinaison Abandoned CA2407714A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US13157499P 1999-04-29 1999-04-29
US60/131,574 1999-04-29
DKPA199900612 1999-05-06
DKPA199900612 1999-05-06
PCT/DK2000/000216 WO2000066627A1 (fr) 1999-04-29 2000-04-28 Expression de la proteine de fixation d'heparine dans des cellules mammiferes de recombinaison

Publications (1)

Publication Number Publication Date
CA2407714A1 true CA2407714A1 (fr) 2000-11-09

Family

ID=26064312

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002407714A Abandoned CA2407714A1 (fr) 1999-04-29 2000-04-28 Expression de la proteine de fixation d'heparine dans des cellules mammiferes de recombinaison

Country Status (13)

Country Link
EP (1) EP1179017A1 (fr)
JP (1) JP2002542813A (fr)
KR (1) KR20020034073A (fr)
CN (1) CN1357007A (fr)
AU (1) AU4286100A (fr)
CA (1) CA2407714A1 (fr)
EA (1) EA200101093A1 (fr)
HK (1) HK1046005A1 (fr)
IL (1) IL146128A0 (fr)
NO (1) NO20015181L (fr)
NZ (1) NZ515762A (fr)
PL (1) PL351499A1 (fr)
WO (1) WO2000066627A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003080660A2 (fr) * 2002-03-27 2003-10-02 Leukotech A/S Procede de preparation d'une proteine de liaison a l'heparine (hbp) mammifere recombinee
EP1739179A1 (fr) * 2005-06-30 2007-01-03 Octapharma AG Transfection stable exempt de sérum et production des protéines recombinantes humaines dans des lignées cellulaires humaines
GB201102108D0 (en) * 2011-02-07 2011-03-23 Hansa Medical Ab Diagnostic method
CN110643586B (zh) * 2019-10-15 2022-06-10 山东大学 一种肝素骨架合酶及其编码基因与应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989008666A1 (fr) * 1988-03-17 1989-09-21 Novo-Nordisk A/S Proteines liant l'heparine, adn codant pour ces proteines, leurs procedes de production ainsi que des preparations therapeutiques les contenant
FI964227L (fi) * 1994-04-21 1996-10-21 Novo Nordisk As Hepariinia sitova proteiini verenmyrkytyksen hoitoon ja menetelmät sen valmistamiseksi
WO1999000417A1 (fr) * 1997-06-25 1999-01-07 Novo Nordisk A/S Procede d'obtention de proteines fixant l'heparine dans des cellules de mammiferes

Also Published As

Publication number Publication date
EA200101093A1 (ru) 2002-04-25
NO20015181D0 (no) 2001-10-23
WO2000066627A1 (fr) 2000-11-09
EP1179017A1 (fr) 2002-02-13
JP2002542813A (ja) 2002-12-17
HK1046005A1 (zh) 2002-12-20
PL351499A1 (en) 2003-04-22
NZ515762A (en) 2002-11-26
CN1357007A (zh) 2002-07-03
NO20015181L (no) 2001-12-20
KR20020034073A (ko) 2002-05-08
AU4286100A (en) 2000-11-17
IL146128A0 (en) 2002-07-25

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Legal Events

Date Code Title Description
FZDE Discontinued