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CA2406643A1 - Promoteurs naturels destines a l'expression genique et au controle metabolique de l'espece bacillus - Google Patents

Promoteurs naturels destines a l'expression genique et au controle metabolique de l'espece bacillus Download PDF

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Publication number
CA2406643A1
CA2406643A1 CA002406643A CA2406643A CA2406643A1 CA 2406643 A1 CA2406643 A1 CA 2406643A1 CA 002406643 A CA002406643 A CA 002406643A CA 2406643 A CA2406643 A CA 2406643A CA 2406643 A1 CA2406643 A1 CA 2406643A1
Authority
CA
Canada
Prior art keywords
bacillus
gene
genes
nucleic acid
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002406643A
Other languages
English (en)
Inventor
Laura A. Bedzyk
Tao Wang
Rick W. Ye
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2406643A1 publication Critical patent/CA2406643A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus
    • Y10S435/833—Bacillus brevis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention a permis d'identifier des gènes du génome <i>Bacillus</i> réagissant à diverses conditions métaboliques et à diverses modifications du cycle de croissance. Lesdits gènes, du fait de cette nouvelle sensibilité, peuvent donc être utilisés dans l'expression génique régulée de <i>Bacillus</i> sp. et le contrôle de la santé des bioréacteurs.
CA002406643A 2000-06-29 2001-06-29 Promoteurs naturels destines a l'expression genique et au controle metabolique de l'espece bacillus Abandoned CA2406643A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US21496700P 2000-06-29 2000-06-29
US60/214,967 2000-06-29
US26832001P 2001-02-13 2001-02-13
US60/268,320 2001-02-13
PCT/US2001/020873 WO2002002766A2 (fr) 2000-06-29 2001-06-29 Promoteurs naturels destines a l'expression genique et au controle metabolique de l'espece bacillus

Publications (1)

Publication Number Publication Date
CA2406643A1 true CA2406643A1 (fr) 2002-01-10

Family

ID=26909551

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002406643A Abandoned CA2406643A1 (fr) 2000-06-29 2001-06-29 Promoteurs naturels destines a l'expression genique et au controle metabolique de l'espece bacillus

Country Status (5)

Country Link
US (2) US6617148B2 (fr)
EP (1) EP1294909A2 (fr)
JP (1) JP2005503752A (fr)
CA (1) CA2406643A1 (fr)
WO (1) WO2002002766A2 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100803095B1 (ko) 2006-11-10 2008-02-18 제주대학교 산학협력단 바실러스 서브틸리스 ｃｈ２ 유래의 키토산아제를 코딩하는유전자, 이 유전자를 포함하는 발현벡터, 이 발현벡터로형질전환된 형질전환체 및 이로부터 생산되는 단백질의정제방법
JP2011067095A (ja) 2008-01-10 2011-04-07 Ajinomoto Co Inc 発酵法による目的物質の製造法
WO2011015327A1 (fr) * 2009-08-03 2011-02-10 C-Lecta Gmbh Procédé de production de nucléases d'une bactérie gram négative par l'utilisation d'un hôte d'expression gram positif
US20130203055A1 (en) * 2010-06-03 2013-08-08 Joan Aurich-Costa Methods and kits for in situ detection of nucleotide sequences
BR112014014307A2 (pt) 2011-12-12 2019-09-24 Cellay Inc métodos e kits para detecção in situ à temperatura ambiente de um ácido nucleico alvo em uma amostra biológica
DK3089991T3 (da) * 2013-12-31 2019-12-02 Danisco Us Inc Forbedret proteinekspression
JP2018139542A (ja) * 2017-02-28 2018-09-13 株式会社Ｍｉｚｋａｎ Ｈｏｌｄｉｎｇｓ 新規温度感受性納豆菌、及び胞子低含有納豆
WO2023137264A1 (fr) * 2022-01-13 2023-07-20 Danisco Us Inc. Compositions et procédés de production améliorée de protéines dans des cellules bactériennes à gram positif

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988002025A1 (fr) 1986-09-17 1988-03-24 Sri International Vecteurs d'expression de bacilles
US5162207A (en) 1989-07-07 1992-11-10 E. I. Du Pont De Nemours And Company Sucrose inducible expression vectors for bacillus sp.
IT1231016B (it) 1989-07-27 1991-11-08 Eniricerche Spa Espressione termoinducibile di geni eterologhi in bacillus subtilis e mezzi e metodi per il suo ottenimento
WO1992014826A1 (fr) 1991-02-15 1992-09-03 Ciba-Geigy Ag PROMOTEUR DE $i(BACILLUS THURINGIENSIS)

Also Published As

Publication number Publication date
WO2002002766A3 (fr) 2003-01-09
EP1294909A2 (fr) 2003-03-26
WO2002002766A2 (fr) 2002-01-10
US6617148B2 (en) 2003-09-09
JP2005503752A (ja) 2005-02-10
US20020155612A1 (en) 2002-10-24
US20040009556A1 (en) 2004-01-15

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