CA2401965A1 - Production d'anticorps dans des plastes transgeniques - Google Patents
Production d'anticorps dans des plastes transgeniques Download PDFInfo
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- CA2401965A1 CA2401965A1 CA002401965A CA2401965A CA2401965A1 CA 2401965 A1 CA2401965 A1 CA 2401965A1 CA 002401965 A CA002401965 A CA 002401965A CA 2401965 A CA2401965 A CA 2401965A CA 2401965 A1 CA2401965 A1 CA 2401965A1
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- plastid
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- immunoglobulin
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
- C07K16/1267—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria
- C07K16/1275—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria from Streptococcus (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8214—Plastid transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
- C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/13—Immunoglobulins specific features characterized by their source of isolation or production isolated from plants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Pharmacology & Pharmacy (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
L'invention concerne des compositions et des méthodes de transformation des plastes de cellules végétales avec de multiples gènes, et d'association ou d'assemblage des protéines multimériques hétérologues des plastes des cellules végétales. L'invention concerne également un produit de synthèse plasmidique codant pour tous les composants polypeptidiques individuels de la protéine multimérique. L'intégration stable des séquences de codage hétérologues dans le génome plastidial de la plante cible s'effectue par recombinaison homologue. La présente invention concerne l'assemblage de chaînes lourdes et légères d'immunoglobuline, avec liaison covalente entre les chaînes, en immunoglobulines actives d'un point de vue immunologique dans le chloroplaste.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US18566100P | 2000-02-29 | 2000-02-29 | |
| US60/185,661 | 2000-02-29 | ||
| PCT/US2001/006274 WO2001064929A1 (fr) | 2000-02-29 | 2001-02-28 | Production d'anticorps dans des plastes transgeniques |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2401965A1 true CA2401965A1 (fr) | 2001-09-07 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002401965A Abandoned CA2401965A1 (fr) | 2000-02-29 | 2001-02-28 | Production d'anticorps dans des plastes transgeniques |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP1268832A4 (fr) |
| AU (1) | AU2001245358A1 (fr) |
| CA (1) | CA2401965A1 (fr) |
| WO (1) | WO2001064929A1 (fr) |
Families Citing this family (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2278523C (fr) | 1997-01-17 | 2013-06-18 | The Scripps Research Institute | Proteine de liaison a l'arn et site de liaison utile a l'expression de molecules de recombinaison |
| US20100251425A9 (en) | 1998-05-15 | 2010-09-30 | University Of Central Florida | Expression of human interferon in transgenic chloroplasts |
| CA2402066C (fr) * | 2000-03-01 | 2015-09-29 | Auburn University | Proteines pharmaceutiques, agents therapeutiques humains, albumine serique humaine, insuline, et toxique b de cholera natif soumis a des plastes transgeniques |
| WO2004005521A1 (fr) | 2002-07-03 | 2004-01-15 | University Of Central Florida | Expression de igf-1 humain dans des plastes transgeniques |
| ES2332872T3 (es) | 2000-04-28 | 2010-02-15 | Planet Biotechnology, Inc. | Inmunoadhesina para la prevencion de la infeccion por rinovirus. |
| US20020168367A1 (en) * | 2000-04-28 | 2002-11-14 | Planet Biotechnology Incorporated | Novel immunoadhesins for treating and preventing viral and bacterial diseases |
| EP1576101A4 (fr) * | 2002-04-23 | 2007-10-31 | Scripps Research Inst | Expression de polypeptides dans des chloroplastes, et compositions et procedes permettant de les exprimer |
| AU2008202053B2 (en) * | 2002-04-23 | 2012-11-01 | The Scripps Research Institute | Expression of polypeptides in chloroplasts, and compositions and methods for expressing same |
| SI1523496T1 (sl) | 2002-07-18 | 2011-11-30 | Merus B V | Rekombinantno proizvajanje zmesi protiteles |
| USRE47770E1 (en) | 2002-07-18 | 2019-12-17 | Merus N.V. | Recombinant production of mixtures of antibodies |
| EP2395017A3 (fr) | 2003-05-30 | 2012-12-19 | Merus B.V. | Conception et utilisation de régions variables appariées de molécules de liaison spécifiques |
| US20100069614A1 (en) | 2008-06-27 | 2010-03-18 | Merus B.V. | Antibody producing non-human mammals |
| EP1737971B1 (fr) | 2004-01-20 | 2017-08-16 | Merus N.V. | Mélanges de proteines de liason |
| WO2006066340A1 (fr) * | 2004-12-21 | 2006-06-29 | Grain Biotech Australia Pty Ltd | Procede permettant l'expression elevee d'immunoglobuline dans des vegetaux |
| AU2006309281B2 (en) | 2005-05-27 | 2011-11-03 | University Of Central Florida | Chloroplasts engineered to express pharmaceutical proteins |
| AU2008232543B2 (en) | 2007-03-30 | 2013-01-17 | University Of Central Florida Research Foundation, Inc. | Chloroplasts engineered to express pharmaceutical proteins in edible plants |
| DK2162537T3 (da) | 2007-06-01 | 2012-04-23 | Sapphire Energy Inc | Anvendelse af genetisk modificerede organismer til at frembringe biomasse-nedbrydende enzymer. |
| EP2203542A1 (fr) | 2007-09-11 | 2010-07-07 | Sapphire Energy, Inc. | Procédés de fabrication de produits organiques avec des organismes photosynthétiques, produits obtenus et compositions pour l'obtention de ceux-ci |
| US10689633B2 (en) | 2008-02-29 | 2020-06-23 | The Trustees Of The University Of Pennsylvania | Expression of β-mannanase in chloroplasts and its utilization in lignocellulosic woody biomass hydrolysis |
| CA2780362C (fr) | 2009-11-09 | 2019-12-24 | University Of Central Florida Research Foundation, Inc. | Administration par voie orale d'agents de tolerance exprimes chez les plantes |
| US20130045492A1 (en) | 2010-02-08 | 2013-02-21 | Regeneron Pharmaceuticals, Inc. | Methods For Making Fully Human Bispecific Antibodies Using A Common Light Chain |
| US9796788B2 (en) | 2010-02-08 | 2017-10-24 | Regeneron Pharmaceuticals, Inc. | Mice expressing a limited immunoglobulin light chain repertoire |
| SMT201900372T1 (it) | 2010-02-08 | 2019-09-09 | Regeneron Pharma | Topo con catena leggera comune |
| EP2683735A1 (fr) | 2011-03-10 | 2014-01-15 | HCO Antibody, Inc. | Molécules de type anticorps tricaténaires bispécifiques |
| WO2012122512A1 (fr) | 2011-03-10 | 2012-09-13 | Hco Antibody, Inc. | Production recombinante de mélanges d'anticorps monocaténaires |
| HUE068728T2 (hu) | 2011-08-05 | 2025-01-28 | Regeneron Pharma | Humanizált univerzális könnyûláncú egerek |
| WO2013063049A1 (fr) | 2011-10-24 | 2013-05-02 | University Of Central Florida Research Foundation, Inc. | Sous-motif d'exendine 4 de la toxine cholérique b exprimé dans un plastide administré par voie orale pour le traitement du diabète de type 2 |
| ES2743399T3 (es) | 2012-04-20 | 2020-02-19 | Merus Nv | Métodos y medios para la producción de moléculas heterodiméricas similares a Ig |
| RU2016141307A (ru) | 2014-03-21 | 2018-04-24 | Регенерон Фармасьютикалз, Инк. | Отличные от человека животные, которые вырабатывают однодоменные связывающие белки |
| US11111314B2 (en) | 2015-03-19 | 2021-09-07 | Regeneron Pharmaceuticals, Inc. | Non-human animals that select for light chain variable regions that bind antigen |
| US20230126423A1 (en) * | 2020-03-02 | 2023-04-27 | Ventria Bioscience Inc. | Enhanced expression yield of immunoglobulin a in eukaryotes |
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| US5451513A (en) * | 1990-05-01 | 1995-09-19 | The State University of New Jersey Rutgers | Method for stably transforming plastids of multicellular plants |
| US5877402A (en) * | 1990-05-01 | 1999-03-02 | Rutgers, The State University Of New Jersey | DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein |
| US5576198A (en) * | 1993-12-14 | 1996-11-19 | Calgene, Inc. | Controlled expression of transgenic constructs in plant plastids |
| US5925806A (en) * | 1995-06-06 | 1999-07-20 | Mcbride; Kevin E. | Controlled expression of transgenic constructs in plant plastids |
| CA2278523C (fr) * | 1997-01-17 | 2013-06-18 | The Scripps Research Institute | Proteine de liaison a l'arn et site de liaison utile a l'expression de molecules de recombinaison |
| NZ502742A (en) * | 1997-08-07 | 2004-04-30 | Univ Auburn | Universal chloroplast integration and expression vectors, transformed plants and products thereof |
| AR020090A1 (es) * | 1998-06-15 | 2002-04-10 | John Innes Ct | Celula vegetal monocotiledonea o semilla de ella,planta o parte de una planta, metodo para hacer una celula vegetal monocotiledonea, metodo para hacer unaplanta, uso de una construccion de expresion para producir una celula de planta o semilla monocotiledonea, y uso de una construccion de expresion |
| PT1097211E (pt) * | 1998-07-10 | 2007-07-12 | Calgene Llc | Expressão de péptidos eucarióticos em plastos vegetais |
| CA2344269A1 (fr) * | 1998-10-07 | 2000-04-13 | Syngenta Participations Ag | Proteines vegetales a action therapeutique |
-
2001
- 2001-02-28 CA CA002401965A patent/CA2401965A1/fr not_active Abandoned
- 2001-02-28 EP EP01918262A patent/EP1268832A4/fr not_active Withdrawn
- 2001-02-28 WO PCT/US2001/006274 patent/WO2001064929A1/fr not_active Ceased
- 2001-02-28 AU AU2001245358A patent/AU2001245358A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2001064929A1 (fr) | 2001-09-07 |
| EP1268832A1 (fr) | 2003-01-02 |
| EP1268832A4 (fr) | 2003-04-02 |
| AU2001245358A1 (en) | 2001-09-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |