CA2401819A1 - Dispositif et procede de preparation d'echantillon pour analyse - Google Patents
Dispositif et procede de preparation d'echantillon pour analyse Download PDFInfo
- Publication number
- CA2401819A1 CA2401819A1 CA002401819A CA2401819A CA2401819A1 CA 2401819 A1 CA2401819 A1 CA 2401819A1 CA 002401819 A CA002401819 A CA 002401819A CA 2401819 A CA2401819 A CA 2401819A CA 2401819 A1 CA2401819 A1 CA 2401819A1
- Authority
- CA
- Canada
- Prior art keywords
- sample
- channels
- valves
- channel
- subsamples
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000034 method Methods 0.000 title claims abstract description 41
- 238000004458 analytical method Methods 0.000 title claims description 19
- 238000010926 purge Methods 0.000 claims abstract description 31
- 238000004891 communication Methods 0.000 claims abstract description 22
- 239000012530 fluid Substances 0.000 claims abstract description 20
- 239000000523 sample Substances 0.000 claims description 130
- 239000007788 liquid Substances 0.000 claims description 21
- 230000002209 hydrophobic effect Effects 0.000 claims description 18
- 238000003752 polymerase chain reaction Methods 0.000 claims description 17
- 238000000151 deposition Methods 0.000 claims description 11
- 239000000758 substrate Substances 0.000 claims description 10
- 239000012472 biological sample Substances 0.000 claims description 8
- 229920000544 Gore-Tex Polymers 0.000 claims description 3
- 230000003213 activating effect Effects 0.000 claims 2
- 238000003825 pressing Methods 0.000 claims 2
- 238000007789 sealing Methods 0.000 claims 1
- 238000005194 fractionation Methods 0.000 abstract description 69
- 238000007600 charging Methods 0.000 abstract description 20
- 238000005119 centrifugation Methods 0.000 abstract description 5
- 239000000463 material Substances 0.000 description 11
- 150000007523 nucleic acids Chemical class 0.000 description 11
- 238000001514 detection method Methods 0.000 description 10
- 238000009826 distribution Methods 0.000 description 10
- 108020004707 nucleic acids Proteins 0.000 description 10
- 102000039446 nucleic acids Human genes 0.000 description 10
- 108020004414 DNA Proteins 0.000 description 9
- 239000012634 fragment Substances 0.000 description 8
- 238000013022 venting Methods 0.000 description 8
- 238000007847 digital PCR Methods 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 6
- 230000001413 cellular effect Effects 0.000 description 5
- 239000012528 membrane Substances 0.000 description 5
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 5
- 239000004810 polytetrafluoroethylene Substances 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 4
- 239000011248 coating agent Substances 0.000 description 4
- 238000000576 coating method Methods 0.000 description 4
- 238000002955 isolation Methods 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 230000008021 deposition Effects 0.000 description 3
- 239000011148 porous material Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 108091093088 Amplicon Proteins 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 239000012807 PCR reagent Substances 0.000 description 2
- 206010036790 Productive cough Diseases 0.000 description 2
- 239000004809 Teflon Substances 0.000 description 2
- 229920006362 Teflon® Polymers 0.000 description 2
- 102000044209 Tumor Suppressor Genes Human genes 0.000 description 2
- 108700025716 Tumor Suppressor Genes Proteins 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 150000002739 metals Chemical class 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 210000003802 sputum Anatomy 0.000 description 2
- 208000024794 sputum Diseases 0.000 description 2
- 238000005309 stochastic process Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 102000043276 Oncogene Human genes 0.000 description 1
- 108700020796 Oncogene Proteins 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000003103 bodily secretion Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- -1 but not limited to Substances 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000013399 early diagnosis Methods 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 238000000608 laser ablation Methods 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 210000002751 lymph Anatomy 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 239000011800 void material Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0241—Drop counters; Drop formers
- B01L3/0265—Drop counters; Drop formers using valves to interrupt or meter fluid flow, e.g. using solenoids or metering valves
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0605—Metering of fluids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00178—Special arrangements of analysers
- G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks
- G01N2035/00247—Microvalves
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Fluid Mechanics (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Sampling And Sample Adjustment (AREA)
- Analysing Materials By The Use Of Radiation (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
L'invention concerne un dispositif et des procédés permettant de diviser un échantillon, notamment un échantillon liquide, en une pluralité de sous-échantillons sans pipetage ni autre opération manuelle. L'invention est particulièrement utile pour isoler des entités dont la présence est soupçonnée dans un échantillon, en vue d'une analyse individuelle.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US51455900A | 2000-02-28 | 2000-02-28 | |
| US09/514,559 | 2000-02-28 | ||
| PCT/US2001/006118 WO2001064950A2 (fr) | 2000-02-28 | 2001-02-27 | Dispositif et procede de preparation d'echantillon pour analyse |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2401819A1 true CA2401819A1 (fr) | 2001-09-07 |
Family
ID=24047724
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002401819A Abandoned CA2401819A1 (fr) | 2000-02-28 | 2001-02-27 | Dispositif et procede de preparation d'echantillon pour analyse |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP1261739A2 (fr) |
| AU (1) | AU2001239886A1 (fr) |
| CA (1) | CA2401819A1 (fr) |
| WO (1) | WO2001064950A2 (fr) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6818404B2 (en) | 1997-10-23 | 2004-11-16 | Exact Sciences Corporation | Methods for detecting hypermethylated nucleic acid in heterogeneous biological samples |
| CA2366778C (fr) | 1999-04-09 | 2008-07-22 | Exact Sciences Corporation | Procedes de detection d'acides nucleiques revelateurs de cancer |
| US6849403B1 (en) | 1999-09-08 | 2005-02-01 | Exact Sciences Corporation | Apparatus and method for drug screening |
| US6919174B1 (en) | 1999-12-07 | 2005-07-19 | Exact Sciences Corporation | Methods for disease detection |
| EP1238113B1 (fr) | 1999-12-07 | 2010-02-24 | EXACT Sciences Corporation | Detection de neoplasmes du poumon dans les selles |
| SE0004351D0 (sv) * | 2000-11-27 | 2000-11-27 | Helen Andersson | System och metod för tidstyrd vätskehantering för reaktioner och processer i ett mikrofluidiskt flödescellsystem |
| US6911308B2 (en) | 2001-01-05 | 2005-06-28 | Exact Sciences Corporation | Methods for detecting, grading or monitoring an H. pylori infection |
| US9109256B2 (en) | 2004-10-27 | 2015-08-18 | Esoterix Genetic Laboratories, Llc | Method for monitoring disease progression or recurrence |
| US9777314B2 (en) | 2005-04-21 | 2017-10-03 | Esoterix Genetic Laboratories, Llc | Analysis of heterogeneous nucleic acid samples |
| US20210387176A1 (en) * | 2020-06-16 | 2021-12-16 | Sani-Tech West, Inc. | Closed fluid receiving and sampling container |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5182082A (en) * | 1991-01-23 | 1993-01-26 | Becton, Dickinson And Company | Multiple aliquot device for distributing a liquid solution into a well |
| US5798215A (en) * | 1993-02-18 | 1998-08-25 | Biocircuits Corporation | Device for use in analyte detection assays |
| US6001307A (en) * | 1996-04-26 | 1999-12-14 | Kyoto Daiichi Kagaku Co., Ltd. | Device for analyzing a sample |
| GB9809943D0 (en) * | 1998-05-08 | 1998-07-08 | Amersham Pharm Biotech Ab | Microfluidic device |
| CA2320296A1 (fr) * | 1998-05-18 | 1999-11-25 | University Of Washington | Cartouche d'analyse liquide |
-
2001
- 2001-02-27 AU AU2001239886A patent/AU2001239886A1/en not_active Abandoned
- 2001-02-27 EP EP01914503A patent/EP1261739A2/fr not_active Withdrawn
- 2001-02-27 CA CA002401819A patent/CA2401819A1/fr not_active Abandoned
- 2001-02-27 WO PCT/US2001/006118 patent/WO2001064950A2/fr not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| AU2001239886A1 (en) | 2001-09-12 |
| WO2001064950A3 (fr) | 2002-01-24 |
| EP1261739A2 (fr) | 2002-12-04 |
| WO2001064950A2 (fr) | 2001-09-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20030049659A1 (en) | Devices and methods for isolating samples into subsamples for analysis | |
| US20050136546A1 (en) | Microtiter plate, system and method for processing samples | |
| US7347976B2 (en) | Methods and devices for removal of organic molecules from biological mixtures using a hydrophilic solid support in a hydrophobic matrix | |
| AU674685B2 (en) | Fluid handling in microfabricated analytical devices | |
| JP2675989B2 (ja) | 核酸増幅のための方法および装置 | |
| US5304487A (en) | Fluid handling in mesoscale analytical devices | |
| EP2058664B1 (fr) | Appareil et procédé de manipulation de fluides pour analyse | |
| KR20070027507A (ko) | 핵산 서열 증폭 및 검출 과정을 수행하기 위한 진단 시스템 | |
| KR20120051709A (ko) | 미세유체 장치 및 이의 용도 | |
| US20070184463A1 (en) | Microfluidic device for purifying a biological component using magnetic beads | |
| US20030073110A1 (en) | Method for isolating nucleic acid and a cartridge for chemical reaction and for nucleic acid isolation | |
| JP2007520693A (ja) | 被検体物のマイクロ流体デバイスへの取り込みならびに収納の方法および装置 | |
| EP1579191A2 (fr) | Methode et appareil pour la separation de particules dans un dispositif microfluidique | |
| US20120100600A1 (en) | Apparatus for polynucleotide detection and quantitation | |
| AU2013350823A1 (en) | Handling liquid samples | |
| CA2401819A1 (fr) | Dispositif et procede de preparation d'echantillon pour analyse | |
| EP1565737A2 (fr) | Isolation de spermatozoides parmi d'autres matieres biologiques au moyen de dispositifs microfabriques et methodes associees | |
| US20250076315A1 (en) | Systems and methods for generating droplets and performing digital analyses | |
| EP3233232A1 (fr) | Procédé et système permettant une séparation flottante | |
| US8012434B2 (en) | Anti-clogging device and method for in-gel digestion applications | |
| WO2010075199A2 (fr) | Dispositif et procédé de manipulation de fluides pour analyse | |
| GB2394912A (en) | Microreactor with fragmentation chamber | |
| US20040120860A1 (en) | Device and method for the transfer of liquid samples | |
| EP1547691A1 (fr) | Plaque de microtitrage, système et méthode pour le traitement d'échantillons | |
| AU2005234646A1 (en) | Nucleic acid preparation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |