CA2401614C - Parametres d'imagerie et d'analyse de petits objets mobiles tels que des cellules - Google Patents
Parametres d'imagerie et d'analyse de petits objets mobiles tels que des cellules Download PDFInfo
- Publication number
- CA2401614C CA2401614C CA002401614A CA2401614A CA2401614C CA 2401614 C CA2401614 C CA 2401614C CA 002401614 A CA002401614 A CA 002401614A CA 2401614 A CA2401614 A CA 2401614A CA 2401614 C CA2401614 C CA 2401614C
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- CA
- Canada
- Prior art keywords
- light
- imaging system
- detector
- tdi detector
- image
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/2803—Investigating the spectrum using photoelectric array detector
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/2823—Imaging spectrometer
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
- G01N15/147—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N21/85—Investigating moving fluids or granular solids
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/10—Beam splitting or combining systems
- G02B27/14—Beam splitting or combining systems operating by reflection only
- G02B27/145—Beam splitting or combining systems operating by reflection only having sequential partially reflecting surfaces
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/10—Beam splitting or combining systems
- G02B27/14—Beam splitting or combining systems operating by reflection only
- G02B27/148—Beam splitting or combining systems operating by reflection only including stacked surfaces having at least one double-pass partially reflecting surface
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B7/00—Mountings, adjusting means, or light-tight connections, for optical elements
- G02B7/28—Systems for automatic generation of focusing signals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/02—Investigating particle size or size distribution
- G01N2015/0294—Particle shape
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
- G01N2015/1472—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle with colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1486—Counting the particles
Landscapes
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Optics & Photonics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Dispersion Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
Selon la présente invention, la lumière provenant d'un objet (24) tel qu'une cellule se déplaçant à travers un système d'imagerie (20) est recueillie et dispersée de sorte qu'elle peut être représentée sur un détecteur de retard et d'intégration (<i>time delay and integration</i>/ TDI) (44). La lumière peut être émise par un objet lumineux ou peut être la lumière provenant d'une source de lumière, qui a été diffusée ou non absorbée par l'objet ou peut comprendre une émission lumineuse provenant d'un ou de plusieurs échantillons à l'intérieur ou sur l'objet. De multiples objets passant à travers le système d'imagerie (20) peuvent faire l'objet de l'imagerie pour obtenir à la fois des images de diffusion et des images de dispersion à différents endroits sur un ou plusieurs détecteurs TDI (44).
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/490,478 | 2000-01-24 | ||
| US09/490,478 US6249341B1 (en) | 1999-01-25 | 2000-01-24 | Imaging and analyzing parameters of small moving objects such as cells |
| US09/538,604 | 2000-03-29 | ||
| US09/538,604 US6211955B1 (en) | 2000-01-24 | 2000-03-29 | Imaging and analyzing parameters of small moving objects such as cells |
| PCT/US2000/028258 WO2001053783A1 (fr) | 2000-01-24 | 2000-10-12 | Parametres d'imagerie et d'analyse de petits objets mobiles tels que des cellules |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2401614A1 CA2401614A1 (fr) | 2001-07-26 |
| CA2401614C true CA2401614C (fr) | 2009-06-02 |
Family
ID=27050070
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002401614A Expired - Lifetime CA2401614C (fr) | 2000-01-24 | 2000-10-12 | Parametres d'imagerie et d'analyse de petits objets mobiles tels que des cellules |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP1272822A4 (fr) |
| AU (2) | AU2001211994B2 (fr) |
| CA (1) | CA2401614C (fr) |
| WO (1) | WO2001053783A1 (fr) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6583865B2 (en) * | 2000-08-25 | 2003-06-24 | Amnis Corporation | Alternative detector configuration and mode of operation of a time delay integration particle analyzer |
| CN101431371B (zh) * | 2007-11-09 | 2011-12-21 | 华为技术有限公司 | 一种光网络设备信道保护倒换的方法及装置 |
| DE202014010499U1 (de) | 2013-12-17 | 2015-10-20 | Kymab Limited | Targeting von humaner PCSK9 zur Cholesterinbehandlung |
| AT516846B1 (de) * | 2015-05-12 | 2016-09-15 | Avl List Gmbh | Vorrichtung und Verfahren zur Zählung und/oder Messung von Partikeln in einem Fluidstrom |
| JP7348730B2 (ja) * | 2019-02-22 | 2023-09-21 | シスメックス株式会社 | 試料測定装置および試料測定方法 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS49133042A (fr) * | 1973-04-09 | 1974-12-20 | ||
| US4284323A (en) * | 1979-03-28 | 1981-08-18 | International Telephone And Telegraph Corporation | Trichroic mirror |
| US4746798A (en) * | 1986-08-18 | 1988-05-24 | Werkzeugmaschinenfabrik | Compact optical wavelength discriminator radiometer |
| US5141609A (en) * | 1990-11-16 | 1992-08-25 | The Trustees Of The Leland Stanford Junior University | Method and device employing time-delayed integration for detecting sample components after separation |
| US5568315A (en) * | 1991-05-28 | 1996-10-22 | Discovision Associates | Optical beamsplitter |
| JP3344635B2 (ja) | 1993-12-27 | 2002-11-11 | シャープ株式会社 | カラー液晶表示装置 |
| US5760899A (en) | 1996-09-04 | 1998-06-02 | Erim International, Inc. | High-sensitivity multispectral sensor |
| US5754291A (en) * | 1996-09-19 | 1998-05-19 | Molecular Dynamics, Inc. | Micro-imaging system |
| US5926283A (en) | 1997-07-12 | 1999-07-20 | Optical Insights, Llc | Multi-spectral two dimensional imaging spectrometer |
| JP3747621B2 (ja) * | 1998-03-26 | 2006-02-22 | コニカミノルタオプト株式会社 | カラー投影表示装置 |
-
2000
- 2000-10-12 CA CA002401614A patent/CA2401614C/fr not_active Expired - Lifetime
- 2000-10-12 WO PCT/US2000/028258 patent/WO2001053783A1/fr not_active Ceased
- 2000-10-12 EP EP00973491A patent/EP1272822A4/fr not_active Withdrawn
- 2000-10-12 AU AU2001211994A patent/AU2001211994B2/en not_active Expired
- 2000-10-12 AU AU1199401A patent/AU1199401A/xx active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP1272822A4 (fr) | 2008-03-12 |
| AU1199401A (en) | 2001-07-31 |
| WO2001053783A1 (fr) | 2001-07-26 |
| AU2001211994B2 (en) | 2004-11-18 |
| EP1272822A1 (fr) | 2003-01-08 |
| CA2401614A1 (fr) | 2001-07-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKEX | Expiry |
Effective date: 20201013 |