CA2495728A1 - Procede pour identifier des anticorps et/ou des antigenes dans un liquide a tester, notamment lors d'une determination de groupe sanguin - Google Patents
Procede pour identifier des anticorps et/ou des antigenes dans un liquide a tester, notamment lors d'une determination de groupe sanguin Download PDFInfo
- Publication number
- CA2495728A1 CA2495728A1 CA002495728A CA2495728A CA2495728A1 CA 2495728 A1 CA2495728 A1 CA 2495728A1 CA 002495728 A CA002495728 A CA 002495728A CA 2495728 A CA2495728 A CA 2495728A CA 2495728 A1 CA2495728 A1 CA 2495728A1
- Authority
- CA
- Canada
- Prior art keywords
- mixture
- buffer
- reaction
- vessel
- detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract 28
- 238000001514 detection method Methods 0.000 title claims abstract 14
- 239000000427 antigen Substances 0.000 title claims abstract 8
- 102000036639 antigens Human genes 0.000 title claims abstract 8
- 108091007433 antigens Proteins 0.000 title claims abstract 8
- 239000007788 liquid Substances 0.000 title claims abstract 8
- 239000008280 blood Substances 0.000 title 1
- 210000004369 blood Anatomy 0.000 title 1
- 239000000203 mixture Substances 0.000 claims abstract 29
- 239000002245 particle Substances 0.000 claims abstract 13
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims abstract 8
- 238000011534 incubation Methods 0.000 claims abstract 8
- 229920002307 Dextran Polymers 0.000 claims abstract 5
- 239000004471 Glycine Substances 0.000 claims abstract 4
- 239000002202 Polyethylene glycol Substances 0.000 claims abstract 4
- 229920001223 polyethylene glycol Polymers 0.000 claims abstract 4
- 239000001509 sodium citrate Substances 0.000 claims abstract 4
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 claims abstract 4
- 229940038773 trisodium citrate Drugs 0.000 claims abstract 4
- 239000007864 aqueous solution Substances 0.000 claims abstract 3
- 239000012528 membrane Substances 0.000 claims 6
- 235000018102 proteins Nutrition 0.000 claims 6
- 108090000623 proteins and genes Proteins 0.000 claims 6
- 102000004169 proteins and genes Human genes 0.000 claims 6
- 239000011324 bead Substances 0.000 claims 5
- 238000002156 mixing Methods 0.000 claims 3
- 102000009027 Albumins Human genes 0.000 claims 2
- 108010088751 Albumins Proteins 0.000 claims 2
- 108010010803 Gelatin Proteins 0.000 claims 2
- 229920005654 Sephadex Polymers 0.000 claims 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims 2
- 229920001971 elastomer Polymers 0.000 claims 2
- 239000008273 gelatin Substances 0.000 claims 2
- 229920000159 gelatin Polymers 0.000 claims 2
- 235000019322 gelatine Nutrition 0.000 claims 2
- 235000011852 gelatine desserts Nutrition 0.000 claims 2
- 230000000087 stabilizing effect Effects 0.000 claims 2
- 229920000936 Agarose Polymers 0.000 claims 1
- 108010076119 Caseins Proteins 0.000 claims 1
- 229920001917 Ficoll Polymers 0.000 claims 1
- 229920001213 Polysorbate 20 Polymers 0.000 claims 1
- 239000012507 Sephadex™ Substances 0.000 claims 1
- 101710120037 Toxin CcdB Proteins 0.000 claims 1
- 229920004890 Triton X-100 Polymers 0.000 claims 1
- 230000001133 acceleration Effects 0.000 claims 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims 1
- 239000005018 casein Substances 0.000 claims 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims 1
- 235000021240 caseins Nutrition 0.000 claims 1
- 210000003743 erythrocyte Anatomy 0.000 claims 1
- 239000000499 gel Substances 0.000 claims 1
- 208000015181 infectious disease Diseases 0.000 claims 1
- 239000004816 latex Substances 0.000 claims 1
- 229920000126 latex Polymers 0.000 claims 1
- 235000011837 pasties Nutrition 0.000 claims 1
- XNGIFLGASWRNHJ-UHFFFAOYSA-N phthalic acid Chemical class OC(=O)C1=CC=CC=C1C(O)=O XNGIFLGASWRNHJ-UHFFFAOYSA-N 0.000 claims 1
- 239000004033 plastic Substances 0.000 claims 1
- 229920003023 plastic Polymers 0.000 claims 1
- 229920002401 polyacrylamide Polymers 0.000 claims 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 238000004062 sedimentation Methods 0.000 claims 1
- 239000000377 silicon dioxide Substances 0.000 claims 1
- 239000007787 solid Substances 0.000 claims 1
- 230000009870 specific binding Effects 0.000 claims 1
- 238000003756 stirring Methods 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
- 238000012800 visualization Methods 0.000 claims 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
La présente invention concerne un procédé pour identifier des anticorps ou des antigènes dans un liquide à tester, par mise en réaction avec un partenaire de liaison spécifique prédéfini. Les antigènes sont liés à un support et le mélange d'échantillon ainsi établi est mis en incubation, puis est centrifugé à travers un mélange particule-tampon. Une agglutination d'antigène à identifier, support et anticorps se forme en cas de réaction antigène-anticorps positive. Une quantité prédéfinie du mélange d'échantillon incubé est ajoutée à une quantité prédéfinie d'un mélange particule-tampon se trouvant à l'intérieur d'un récipient d'identification. Ce mélange particule-tampon présente un tampon d'incorporation constitué d'une solution aqueuse de dextrane et/ou de polyéthylèneglycol, de glycine et/ou de citrate trisodique.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10239568.3 | 2002-08-23 | ||
| DE2002139568 DE10239568A1 (de) | 2002-08-23 | 2002-08-23 | Verfahren zum Nachweis von Antikörpern und/oder Antigenen in einer Testflüssigkeit sowie zur Blutgruppenbestimmung |
| PCT/EP2003/008995 WO2004019038A2 (fr) | 2002-08-23 | 2003-08-13 | Procede pour identifier des anticorps et/ou des antigenes dans un liquide a tester, notamment lors d'une determination de groupe sanguin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2495728A1 true CA2495728A1 (fr) | 2004-03-04 |
| CA2495728C CA2495728C (fr) | 2012-08-21 |
Family
ID=31197475
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2495728A Expired - Fee Related CA2495728C (fr) | 2002-08-23 | 2003-08-13 | Procede pour identifier des anticorps et/ou des antigenes dans un liquide a tester, notamment lors d'une determination de groupe sanguin |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP1532454A2 (fr) |
| AU (1) | AU2003260413A1 (fr) |
| CA (1) | CA2495728C (fr) |
| DE (1) | DE10239568A1 (fr) |
| WO (1) | WO2004019038A2 (fr) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102445550A (zh) * | 2010-10-09 | 2012-05-09 | 苏州苏大赛尔免疫生物技术有限公司 | ABO、RhD血型定型试剂卡及其制备方法及其采用的抗体稀释液 |
| CN105628940A (zh) * | 2015-12-30 | 2016-06-01 | 合肥天一生物技术研究所 | 一种abo亚型血型检测卡 |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102006024927B4 (de) * | 2006-05-25 | 2008-12-11 | DRK-Blutspendedienst Baden-Württemberg-Hessen gemeinnützige GmbH | Verfahren zum Nachweis von Antikörpern und/oder Antigenen sowie zur Blutgruppenbestimmung in einer Testsubstanz |
| CN103926415B (zh) * | 2014-03-24 | 2015-11-25 | 上海市血液中心 | 人血液中血型抗体联合快速检测体系 |
| DE102017114537A1 (de) * | 2017-06-29 | 2019-01-03 | Endress+Hauser Conducta Gmbh+Co. Kg | Sensormembran, Sensorkappe und optischer Sensor |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5552064A (en) * | 1993-02-26 | 1996-09-03 | Ortho Diagnostic Systems, Inc. | Column agglutination assay and device using biphasic centrifugation |
| US5905028A (en) * | 1994-05-17 | 1999-05-18 | Gamma Biologicals, Inc. | Method and apparatus useful for detecting bloodgroup antigens and antibodies |
| EP0849595B1 (fr) * | 1996-12-18 | 2001-05-09 | Stiftung Für Diagnostische Forschung | Utilisations des particules synthétiques comme réactif d'agglutination |
| ES2126521B1 (es) * | 1997-06-05 | 1999-11-16 | Transfusion De La Comunidad Va | Metodo para la deteccion de antigenos presentes en la membrana de hematies, propios o acoplados y de anticuerpos irregulares en muestras de suero. |
-
2002
- 2002-08-23 DE DE2002139568 patent/DE10239568A1/de not_active Ceased
-
2003
- 2003-08-13 CA CA2495728A patent/CA2495728C/fr not_active Expired - Fee Related
- 2003-08-13 WO PCT/EP2003/008995 patent/WO2004019038A2/fr not_active Ceased
- 2003-08-13 AU AU2003260413A patent/AU2003260413A1/en not_active Abandoned
- 2003-08-13 EP EP03792313A patent/EP1532454A2/fr not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102445550A (zh) * | 2010-10-09 | 2012-05-09 | 苏州苏大赛尔免疫生物技术有限公司 | ABO、RhD血型定型试剂卡及其制备方法及其采用的抗体稀释液 |
| CN102445550B (zh) * | 2010-10-09 | 2014-02-05 | 苏州苏大赛尔免疫生物技术有限公司 | 一种ABO、RhD血型定型试剂卡及其制备方法 |
| CN105628940A (zh) * | 2015-12-30 | 2016-06-01 | 合肥天一生物技术研究所 | 一种abo亚型血型检测卡 |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2003260413A1 (en) | 2004-03-11 |
| DE10239568A1 (de) | 2004-03-04 |
| CA2495728C (fr) | 2012-08-21 |
| EP1532454A2 (fr) | 2005-05-25 |
| WO2004019038A3 (fr) | 2004-04-22 |
| WO2004019038A2 (fr) | 2004-03-04 |
| AU2003260413A8 (en) | 2004-03-11 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |
Effective date: 20160815 |